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Cloning vector pUC19, complete sequenceGenBank: M77789.2

FASTA Graphics Go to: 

LOCUS SYNPUC19V 2686 bp DNA circular SYN 07-AUG-2008DEFINITION Cloning vector pUC19, complete sequence.ACCESSION M77789 M11662VERSION M77789.2 GI:6691170KEYWORDS .SOURCE Cloning vector pUC19ORGANISM Cloning vector pUC19 

other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 2686)AUTHORS Norrander,J., Kempe,T. and Messing,J.TITLE Construction of improved M13 vectors using

oligodeoxynucleotide-directed mutagenesisJOURNAL Gene 26 (1), 101-106 (1983)PUBMED 6323249 

REFERENCE 2 (bases 1 to 2686)AUTHORS Yanisch-Perron,C., Vieira,J. and Messing,J.TITLE Improved M13 phage cloning vectors and host strains:

nucleotidesequences of the M13mp18 and pUC19 vectors

JOURNAL Gene 33 (1), 103-119 (1985)PUBMED 2985470 REMARK Erratum:[Gene. 1992 May 1;114(1):81-3. PMID: 1375182]

REFERENCE 3 (bases 1 to 2686)AUTHORS Messing,J.TITLE Direct SubmissionJOURNAL Submitted (27-SEP-1991) Waksman Institute, Rutgers State

Univ., 190Frelinghuysen Road, Piscataway, NJ 08854, USA

REFERENCE 4 (bases 1 to 2686)AUTHORS Messing,J.TITLE Direct SubmissionJOURNAL Submitted (12-JAN-2000) Waksman Institute, Rutgers State

Univ., 190Frelinghuysen Road, Piscataway, NJ 08854, USA

REMARK Sequence update by submitterCOMMENT On Jan 12, 2000 this sequence version replaced gi:209214.FEATURES Location/Qualifiers

source 1..2686/organism="Cloning vector pUC19"/mol_type="other DNA"/strain="ATCC 37254"/culture_collection="ATCC:37254"/db_xref="taxon:31851"

misc_feature  1..447/note="M13mp19"

misc_feature  1..230/note="Lac-operon"

misc_feature  233..289/note="polylinker of M13mp19;

http://www.ncbi.nlm.nih.gov/nuccore/6691170?report=fastahttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170#goto6691170_0http://www.ncbi.nlm.nih.gov/nuccore/6691170#goto6691170_0http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=31851http://www.ncbi.nlm.nih.gov/pubmed/6323249http://www.ncbi.nlm.nih.gov/pubmed/2985470http://www.ncbi.nlm.nih.gov/nuccore/209214http://www.ncbi.nlm.nih.gov/nuccore/209214http://www.atcc.org/Products/All/37254.aspxhttp://www.atcc.org/Products/All/37254.aspxhttp://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=31851http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=31851http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=1&to=447&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=1&to=230&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=233&to=289&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=233&to=289&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=1&to=230&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=1&to=447&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=31851http://www.atcc.org/Products/All/37254.aspxhttp://www.ncbi.nlm.nih.gov/nuccore/209214http://www.ncbi.nlm.nih.gov/pubmed/2985470http://www.ncbi.nlm.nih.gov/pubmed/6323249http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=31851http://www.ncbi.nlm.nih.gov/nuccore/6691170#goto6691170_0http://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=fasta

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  HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI"

misc_feature  290..447/note="Lac-Operon"

misc_feature  complement(448..547)/note="pBR322"

misc_feature  complement(548..684)

/note="pBR322"misc_feature  complement(685..2686)

/note="pBR322"ORIGIN

1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaatgcagctggca

61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatgtgagttagct

121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgttgtgtggaat

181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacgccaagcttgc

241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca

ctggccgtcg301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc

cttgcagcac361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc

ccttcccaac421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt

acgcatctgt481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat

gccgcatagt541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct

tgtctgctcc601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt

cagaggtttt661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta

tttttatagg721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg

ggaaatgtgc781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg

ctcatgagac841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt

attcaacatt901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt

gctcacccag961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg

ggttacatcg1021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa

cgttttccaa

1081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtattgacgccgggc

1141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgagtactcaccag

1201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagtgctgccataa

1261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggaggaccgaaggagc

1321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgttgggaaccgg

http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=290&to=447&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=448&to=547&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=548&to=684&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=685&to=2686&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=685&to=2686&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=548&to=684&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=448&to=547&sat=4&sat_key=24004809http://www.ncbi.nlm.nih.gov/nuccore/6691170?from=290&to=447&sat=4&sat_key=24004809

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  1381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgtagcaatggcaa

1441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccggcaacaattaa

1501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcccttccggctg

1561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt

atcattgcag1621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg

gggagtcagg1681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg

attaagcatt1741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa

cttcattttt1801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa

atcccttaac1861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga

tcttcttgag1921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg

ctaccagcgg

1981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaactggcttcagca

2041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccaccacttcaaga

2101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtggctgctgcca

2161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccggataaggcgc

2221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcgaacgacctaca

2281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttcccgaagggagaa

2341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacgagggagcttc

2401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctctgacttgagc

2461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgccagcaacgcgg

2521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttctttcctgcgttat

2581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgataccgctcgccgca

2641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga//

Cloning vector pUC19, complete sequenceGenBank: M77789.2

GenBank Graphics >gi|6691170|gb|M77789.2|SYNPUC19V Cloning vector pUC19, complete sequenceGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAAC

http://www.ncbi.nlm.nih.gov/nuccore/6691170?report=genbankhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=graphhttp://www.ncbi.nlm.nih.gov/nuccore/6691170?report=genbank

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AGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC

CCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTG

AAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA