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Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
RESEARCH ACTIVITIES
Antibiotic Resistance, Antiviral
Resistance, New Tropical
Diseases, Multiresistan
Mycobacteria etc…
Results are published in open
scientific literature
Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
INTERNATIONAL PARTNERSHIPS AND PROJECTS
Capacity – Building
Gap Analysis
Training (E-learning / In-presence) and Sharing Experience
Hands-on courses (BSL2; BSL3 and BSL4) tailored to countries local needs
Workshops and seminars
Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
INTERNATIONAL PARTNERSHIPS
AND PROJECTS – ONGOING
1.The Master Euro-Africain: Biosécurité et
Biosûreté
2.Biorisk Assessment Mission, Tiblisi
3.CBRN CoE Initiative (P3, P6, P7, P13 and
P18)
4.Instrument for Stability
Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
Georgia, Tbilisi
Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
International conference “Biosecurity and Biosafety: future trends and solutions”
Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
Develop a holistic and multidisciplinary approach on bio-related issues:
bio-threats analysis, biosafety, biosecurity, biorisk assessment and management and
biohazardous waste containment
Support and foster a risk mitigation culture
Support the harmonization of international bio-safety and bio-security standards among
the participating countries
Strengthen regional and international collaboration and cooperation so as knowledge
sharing and lessons learned exchange
Enhance networking and awareness raising on bio-related issues and and support
synergies among scientific communities and institutions.
Meeting of Experts - Palais des Nations
Geneva 4 - 8 August 2014
CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY
BSL4 FACILITY
L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY)
Prof. Maria Rita Gismondo
Prof. Maria Rita Gismondo, MD
[email protected] Address: Via G. B. Grassi, 74, 20157 Milan
(Italy)
Phone: +39 02 39042239 / +39 02
50319831
Fax: +39 02 50319832
Dott. Francesca Cerutti
“TRAINING ON FIELD”
A practical training for the isolation of
Bacillus anthracis from environmental samples in
high-risk areas
Meeting of Experts
Palais des Nations, Geneva
4 - 8 August 2014.
Antonio Fasanella
Istituto Zooprofilattico Sperimentale di Puglia e Basilicata
Foggia, Italy
NETWORK OF ISTITUTI ZOOPROFILATTICI
SPERIMENTALI
• Control of zoonosis
• Food security programs
• Management of emergency ( brucellosis, tuberculosis,
avian flu, anthrax, blue tongue )
• Research on animal infectious diseases
• Development of vaccines for veterinary use
• Cooperation with national and international scientific
organizations
Anthrax Reference
Institute of Italy
Major tasks of the
Anthrax Reference Institute of Italy
• Anthrax vaccine production
• Molecular epidemiology and genotype database
• Disease control
• Bioterrorism emergency ( Italian Laboratory Reference)
• Animal model
• Research
Anthrax in Italy
The low diversity of B. anthracis genotypes in
Italy suggests a single, dominant historical
introduction, followed by localized
differentiation
In Italy, anthrax is a sporadic
disease. Every year, few
outbreaks occur in the known
risk areas of central-southern
Italy and in the major islands.
Pollino National Park ( Basilicata
Region – South Italy) epidemic map,
with the representation of the single
outbreaks identified by GIS
information. Foggia
During the 2004 summer in the
Pollino National Park in
Basilicata it was registered an
anthrax epidemic that involved
41 farms of 13 towns in an area
of about 900 Km2 in which there
were about 7.000 cattle and
33.000 sheep and goats.
In 4 weeks 81 cattle died, as well as 15 sheep, 9 goats,11 horses and 8 deer.
Epidemic outbreak of 2004
Bovine
Carcass burial Horse
Red Deer
Epidemic outbreak of 2004
More than 17% of the population of deer in the park died during the outbreak of
anthrax
(Fasanella A. et al. 2007 Anthrax in Red Deer (Cervus elaphus), Italy. Emerg Infect Dis. 13(7): 1118–1119)
Epidemic outbreak of 2004
Epidemic outbreak of 2004
Two human suspect cases
Outdoor laboratory
In some restricted areas of the park, for several time were buried animals died of anthrax
Vaccination programs ensure the control of the
disease in animals that graze in that area.
These areas represent a valuable environment for the study of the ecology of
Bacillus anthracis
Since 2010, the Anthrax Reference
Institute of Italy use this outdoor
laboratory for trainings direct to
researchers (from foreign countries)
regarding the technique of isolation of
spores from contaminated environments.
Outdoor laboratory
These training activities have been
continued in the framework of the
subsequent Joint Action QUANDHIP. For
further information on QUANDHIP, please,
see website on next slide.
The “training on field” was included in
the EU funded project EQADeBa, as an
educational activity.
http://www.quandhip.eu/
Training on field: Program
• •First day: – Arrival at the airport of Bari and departure to Pollino National Park . Overnight
• •Second day: – Sampling of soils in areas of National Park. Return to Foggia and overnight
• •Third day: – Processing of collected samples at the BL3 laboratory of the National Reference Centre
for anthrax. Isolation of Bacillus anthracis using GABRI or WHO method
• •Fourth day: – Biomolecular analysis of suspect colonies and final meeting
• • Fifth day: – Departure from Bari airport
Training on field
Processing of collected samples
Training on field
Biomolecular analysis of suspect colonies: reading of plates
List of Institutes that participated in the training
1. Österreichische Agentur für Gesundheit und Ernährungssicherheit (AGES) –
Austria
2. Friedrich-Loeffler-Institut / Bundesforschungsinstitut für Tiergesundheit (FLI) –
Germany
3. FoHM: Folkhälsomyndigheten – Public Healt Agency of Sweden – Sweden
4. Public Healt England – England
5. Polish National Institute of Hygiene (PZH) – Poland
6. Infectiology Centre of Latvia (LIC) – Lithuania
7. Federal Office for Civil Protection (FOCP), Spiez Laboratory – Switzerland
8. Robert Koch Institute – Germany
9. Basurto University Hospital, Bilbao – Spain
10. ANSES – France
11. Faculty of Veterinary Medicine of Tirana – Albania
12. Faculty of Medicine, Mu’tah University, Al-karak – Jordan
13. Biotechnology Institute of a National Academy of Sciences of the Kyrgyz
Republic - Kyrgyzstan
Positive contribution
– Many researchers have the opportunity to observe B. anthracis in its natural environment
– Exchange of opinions and experiences
– Incentive to improve the technique of isolation
– Start of new collaborations
• The aim of the training is to prepare the researchers in the techniques of collection of contaminated soil samples.
• This training aims to verify the contamination level of environment (natural or with suspect of a deliberate release).
• The training is free and is directed to researchers prior approval of the Italian government authorities
Contacts:
Antonio FASANELLA
Angela ACETI
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata
WEB SITE: www.izsfg.it