CELLULOSE ACETATE • REGENERATED CELLULOSE • PVDF • PTFE • POLYPROPYLENE • PES • NYLON

3 Mundells Industrial Centre,Welwyn Garden City,Herts, AL7 1EW

PHONE: +44 (0)1707 394 949 FAX: +44 (0)1707 391311 enqu i r ies@chromaco l .com

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CHROMACOL SYRINGE FILTERS (continued)

PRE-FILTERCATALOG # MEMBRANE DIAMETER PORE SIZE 1.0 µM GLASS

F2504-7 Regenerated Cellulose 4 mm 0.45 µm No

F2504-8 Regenerated Cellulose 4 mm 0.2 µm No

F2513-7 Regenerated Cellulose 17 mm 0.45 µm No

F2513-8 Regenerated Cellulose 17 mm 0.2 µm No

F2500-7 Regenerated Cellulose 30 mm 0.45 µm No

F2500-8 Regenerated Cellulose 30 mm 0.2 µm Yes

NOW CERTIFIED FOR ION CHROMATOGRAPHY

F2513-14 PES (Polyethersulfone) 17 mm 0.45 µm No

F2513-17 PES (Polyethersulfone) 17 mm 0.2 µm No

F2500-14 PES (Polyethersulfone) 30 mm 0.45 µm No

F2500-17 PES (Polyethersulfone) 30 mm 0.2 µm No

F2500-18 GMF (Glass MicroFiber) 30 mm 0.7 µm No

F2500-19 GMF (Glass MicroFiber) 30 mm 1.2 µm No

F2500-20 GMF (Glass MicroFiber) 30 mm 3.1 µm No

F2504-9 Polypropylene 4 mm 0.45 µm No

F2504-10 Polypropylene 4 mm 0.2 µm No

F2513-9 Polypropylene 17 mm 0.45 µm No

F2513-10 Polypropylene 17 mm 0.2 µm No

F2500-9 Polypropylene 30 mm 0.45 µm No

F2500-10 Polypropylene 30 mm 0.2 µm No

F2502-9 Polypropylene 30 mm 0.45 µm Yes

F2504-15 Cellulose Acetate 4 mm 0.45 µm No

F2504-16 Cellulose Acetate 4 mm 0.2 µm No

F2513-15 Cellulose Acetate 17 mm 0.45 µm No

F2513-16 Cellulose Acetate 17 mm 0.2 µm No

F2500-15 Cellulose Acetate 30 mm 0.45 µm No

F2500-16 Cellulose Acetate 30 mm 0.2 µm No

CHROMACOL SYRINGE FILTERS• Assured quality—each lot independently tested for physical properties &

membrane tested for UV extractables• Secure Luer Lok® inlet• Solvent resistant, low extractable Polypropylene Housing• Select Membranes available with 1.0 µm glass pre-filter for

hard-to-filter samples • All Chromacol Syringe filters are packed either 100 to a pack or

500 to a case.

PRE-FILTERCATALOG # MEMBRANE DIAMETER PORE SIZE 1.0 µM GLASS

F2504-1 Nylon 4 mm 0.45 µm No

F2504-2 Nylon 4 mm 0.2 µm No

F2513-1 Nylon 17 mm 0.45 µm No

F2513-2 Nylon 17 mm 0.2 µm No

F2500-1 Nylon 30 mm 0.45 µm No

F2500-2 Nylon 30 mm 0.2 µm No

F2500-12 Nylon 30 mm 1.5 µm No

F2500-50 Nylon 30 mm 5.0 µm No

F2502-1 Nylon 30 mm 0.45 µm Yes

F2502-2 Nylon 30 mm 0.2 µm Yes

F2504-3 PTFE 4 mm 0.45 µm No

F2504-4 PTFE 4 mm 0.2 µm No

F2513-3 PTFE 17 mm 0.45 µm No

F2513-4 PTFE 17 mm 0.2 µm No

F2500-3 PTFE 30 mm 0.45 µm No

F2500-4 PTFE 30 mm 0.2 µm No

F2500-13 PTFE 30 mm 1.0 µm No

F2500-11 PTFE 30 mm 5.0 µm No

F2502-3 PTFE 30 mm 0.45 µm Yes

F2504-5 PVDF 4 mm 0.45 µm No

F2504-6 PVDF 4 mm 0.2 µm No

F2513-5 PVDF 17 mm 0.45 µm No

F2513-6 PVDF 17 mm 0.2 µm No

F2500-5 PVDF 30 mm 0.45 µm No

F2500-6 PVDF 30 mm 0.2 µm No

CHROMACOL HPLC Syringe Filters

Throughput Volume Comparison 30mmChromacol Syringe FiltersOur new filter permits more sample to be processedbefore filter overload. This is a considerable improvementin throughput volume compared to our first generationTarget filters or competitive products.

CHROMACOLI M P R O V E D P E R F O R M A N C E …

Chromacol Syringe Filters havebeen improved for even greaterperformance and samplethroughput. National Scientifics’Continuous ImprovementProgram has resulted in ournew re-designed ChromacolFilters, that now provide industryleading levels in sample loading andfilter efficiency.

Chromacol’s new 30mm and 17mm filter formats providesuperior throughput and sample loading over standard,competitive 25mm and 13mm filters. Now you can processup to 50% more sample before reaching maximum sampleloading for our new 30mm filters.

The new sample distribution rings promote uniform applicationof the sample across the membrane area. This featuremaximizes the available filtration area, speed and reducesbackpressure when filtering highly particulate samples. Ournew, automated, manufacturing process ensures uniformityand reliability more than ever before. Our new methodseliminate variable results through controlled manufacturingconsistency from batch to batch, filter to filter.

Performance Filtration AssuranceImproved Chromacol Filters assure product performance andreproducibility through a vigorous quality testing program thatchallenges each type of filter to provide interference-freeresults. Every production lot is quality monitored:

• All critical filter performance specifications confirmed;bubble point, burst pressure, membrane retention, flow rate.

• HPLC performance and reproducibility; actual NIST-traceable standard run confirmed for baseline, peakarea/shape, retention time.

• Actual filter efficiencies for sample;standard sample spectrophotometrically tested for efficiencyand performance.

• Retention samples maintained for six months; youractual filter lot used to answer application orperformance inquiries.

H P L C Syringe Filters

5

4.5

4

3.5

3

2.5

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Filter Type Volume

Improved Chromacol Nylon, 0.45µm, 30mm, with prefilter 4.71 ± 0.9mLOlder version Chromacol Nylon, 0.45µm, 25mm, prefilter 2.50 ± 0.2mLCompetitive 25mm Nylon, 0.45µm filter 0.49 ± 0.1mL

80

60

40

20

0

1 2 3 4 5

Column: Alltech Altima C18, 4.6 x 250mmTemperature: 25°CFlow Rate: 1.0 mL/min.Detection: 254nmMobile Phase: ACN/H2O (70:30)

Validated HPLC Performance-Caffeine (4µg/mL)

Chromacol’s quality assurance program utilizes a NISTtraceable standard to ensure chromatographic performance.Our enhanced testing goes far beyond industry testing forquiet baselines. Each lot of Chromacol filters is tested forpeak area, retention time, and peak shape of ourtraceable standard. Now you can be assured of industryleading and confirmed chromatographic performance andreproducibility lot to lot and filter to filter.

120

100

80

60

40

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BINDING CHARACTERISTICS of VARIOUS MEMBRANES(Binding in µg/cm2 of G-Globulin)

Flow Rate StudyChromacol’s Syringe Filter’s increased diametermakes quick work of your filtration procedures. Ourimproved diameter permits faster flow rates andminimizes back pressures. Now you can filter fasterand easier than ever before... even faster thanpopular, competitive syringe filters.

Test was performed using aconstant pressure and flowrate pump. Flow pressurewas 25 psi of distilled H20.All filters were Nylon 0.45µmwith prefilters. Collectionvolumes were done postfilter against time expired.Each filter was run four times.

Enhanced quality control and clean room assembly

Chromacol filters are now assembled under clean roomconditions. These new manufacturing controls lessenany airborne product contaminants, humidity andtemperature changes that could adversely affect filterperformance. With our automated procedures, humanhandling is kept to extreme minimums. These newprocedures mean a cleaner, better and more compliantfilter for even the most exacting filtration demands.

Protein Binding of Biological SamplesChromacol’s Regenerated Cellulose syringe filters possesssuperior characteristics for the filtration of biologicalsamples or high binding analytes. Our RegeneratedCellulose filters have low membrane zeta potentials toprovide ultra-low biological based binding coefficients, tomaximize sample yield after filtration. No binders, surfactantsor wetting agents are used, eliminating a source ofcontamination. Hydrophilic Regenerated Cellulose alsoexhibits excellent chemical resistance and high flow rateswhen compared to Polysulfone and Cellulose Acetate filtersnormally used in biological assay filtration.

Flow Rate StudyBrand Filter Dia. Flow RateChromacol 30mm 30mm 186 ml/min

Old style Chromacol 25mm 147ml/min

Competitor G 25mm 170ml/min

no filter in line 235ml/min

Specifications:Membrane: Hydrophilic PolypropyleneHousing: Medical Grade, Virgin Polypropylene

Connections: Enhanced Female Luer-Lok Inlet Male Slip Outlet

Max. Operating Temp. 110°CMax. Operating Pressure: 17mm – 115 psi

30mm – 90 psiRetention Volumes: 17mm – <29 µL

30mm – <137 µL

Polypropylene Specifications Regenerated Cellulose Specifications

Specifications:Membrane: HPLC Certified Regenerated CelluloseHousing: Medical Grade, Virgin PolypropyleneProtein Binding: <5µg/cm2

Connections: Enhanced Female Luer-Lok Inlet Male Slip Outlet

Max. Operating Temp. 110°CMax. Operating Pressure: 4mm – 75psi

17mm – 115 psi30mm – 90 psi

Retention Volumes: 4mm – <15 µL17mm – <29 µL30mm – <137 µL

Autoclave: Sterilize by dry heat at 121°C for 15 min

PVDF Specifications

Specifications:Membrane: HPLC Certified PVDFHousing: Medical Grade, Virgin PolypropylenePrefilter: Binder-Free Glass Microfiber Prefilter

Connections: Enhanced Female Luer-Lok Inlet Male Luer Slip Outlet

Max. Operating Temp. 110°CMax. Operating Pressure: 4mm – 75psi

17mm – 115 psi30mm – 90 psi

Retention Volumes: 4mm – <15 µL17mm – <29 µL30mm – <137 µL

Autoclave: Sterilize by dry heat at 121°C for 15 min

Specifications:Membrane: Binder-free glass microfiberHousing: Medical Grade, Virgin Polypropylene

Connections: Enhanced Female Luer-Lok Inlet Male Luer Slip Outlet

Max. Operating Temp. 110°CMax. Operating Pressure: 30mm – 90 psiRetention Volumes: 30mm – <137 µL

Glass Microfiber (GMF) Specifications

PTFE Specifications

Specifications:Membrane: HPLC Certified PTFE, w/polypropylene supportHousing: Medical Grade, Virgin PolypropylenePrefilter: Binder Free Glass Microfiber

Connections: Enhanced Female Luer-Lok Inlet Male Luer Slip Outlet

Max. Operating Temp. 100°CMax. Operating Pressure: 4mm – 75psi

17mm – 115 psi30mm – 90 psi

Retention Volumes: 4mm – <15 µL17mm – <29 µL30mm – <137 µL

Autoclave: Sterilize by dry heat at 121°C for 15 min

Nylon Specifications

Specifications:Membrane: HPLC Certified NylonHousing: Medical Grade, Virgin PolypropylenePrefilter: Binder Free Glass Microfiber

Connections: Enhanced Female Luer-Lok Inlet Male Slip Outlet

Max. Operating Temp. 100°CMax. Operating Pressure: 4mm – 75psi

17mm – 115 psi30mm – 90 psi

Retention Volumes: 4mm – <15 µL17mm – <29 µL30mm – <137 µL

Autoclave: Sterilize by dry heat at 121°C for 15 min

PES Specifications

Specifications:Membrane: ICP Certified PES (PolyEtherSulfone)Housing: Medical Grade, Virgin PolypropyleneConnections: Enhanced Female Luer-Lok Inlet

Male Slip OutletMax. Operating Temp. 100°CMax. Operating Pressure: 4mm – 75psi

17mm – 115 psi30mm – 90 psi

Retention Volumes: 4mm – <15 µL17mm – <29 µL30mm – <137 µL

Autoclave: Sterilize by dry heat at 121°C for 15 min

Cellulose Acetate Specifications

Specifications:Membrane: HPLC Certified Cellulose AcetateHousing: Medical Grade, Virgin PolypropyleneProtein Binding: <24µg/cm2

Connections: Enhanced Female Luer-Lok Inlet Male Luer Slip Outlet

Max. Operating Temp. 110°CMax. Operating Pressure: 4mm – 75psi

17mm – 115 psi30mm – 90 psi

Retention Volumes: 4mm – <15 µL17mm – <29 µL30mm – <137 µL

Autoclave: Sterilize by dry heat at 121°C for 15 min

SampleDistribution ringsdistribute samplefor maximumspeed and uniformfiltration acrossmembrane surface

Membranetype andporosity clearlyidentified oneach filter

Chemically resistantpolypropylenehousing minimizesextractables

Enhanced female Luer-Lok®

design assures safe andsecure connections

Comprehensive quality systemsensure interference free results

U R L : w w w . c h r o m a c o l . c o m

3 Mundells Industrial Centre,Welwyn Garden City,Herts, AL7 1EW

PHONE:+44 (0)1707 394 949 FAX: +44 (0)1707 391311enqu i r ies@chromaco l .com