China Status Report

De-Zhu Li5 July 2013

iFlora conceptual product

iBOL International Scientific Collaboration Committee Meeting

Myers Myers et alet al. 2000. . 2000. Nature,Nature, 403: 853-858 403: 853-858

China and Southeast Asia are among the bio- diversity hotspots

There are 31 000 species of vascular plants in China

China joined iBOL and became a central node in April 2008

China-Canada meeting, Oct 2008

2008 年 10 月 14日

China National Committee for Barcode of Life

established in June 2008

China-Canada joint meeting on Barcode of Life in

Beijing, October 2008

CBOL Beijing meeting, May 2009

CBOL Beijing meeting, May 2009

China plant DNA barcoding meeting, 11 July 2009,

Beijing

China plant DNA barcoding pilot meeting, 3 May 2009, Beijing

Germplasm Bank of Wild Species (GWOBS) passed national acceptance in Nov 2009

57618 accessions of seeds of >8141 species, and 7000 DNA samples by Dec 2012;Good base for plant DNA barcodingVoucher specimens for some seed collections with only vegetative characters;Big challenges for species identificationThe rapid, efficient and accurate species identification with DNA barcodes for these collections is one of the key tasks for GBOWS.

Kick-off meeting for the project 14th Aug., 2009

Project: Plant DNA barcoding of China based on Germplasm Bank of Wild Species

Research period: July 2009-Dec. 2012Project number: 2009-LSF-GBOWS-01Participant: More than 50 botanists from

22 organizations

Main research contents

To construct standardized methods and

protocols for DNA barcoding To evaluate DNA barcodes for Plant DNA

barcoding To generated DNA barcodes for the seeds in

GBOWS To generate the standardized DNA barcodes

for key and represented taxa To construct DNA barcodes database system.

Project task and objectives

First stage (2009-2012) to generate 72 000 barcodes for 4 500

species, which include 3 600 DNA barcodes for 2 000- 3000 species from GBOWS .

Second stage (2013-2016) to obtain 14 400- 150 000 DNA barcodes

for 9 000 -10 000 spp, which will facility the rapid and accurate species identification for more than one third of Chinese vascular plants.

Project progress

Prepare the methods and protocols for plant DNA barcoding Two training workshops in January and August 2010 in

Kunming

Plant DNA barcoding technique trainingPlant DNA barcoding technique training

Jan. 5, 2010

Discussed the developing strategy of plant DNA barcoding in China after the Mexico meeting.

Attained more DNA barcodes data in a short period;

Evaluation the four DNA barcodes;

To publish ‘high- impact’ papers.

Planning meetings

Jan. 2010, Field expedition24 people, 9 organizationsSoutheastern YunnanSeed plant:1379 records; 3 specimens and 2 DNA samples for each individualBryophyte:426 records,60 % to species

Two papers favored ITS2 published by a group lead

by Chen Shi-Lin of the Institute of Medicinal Plant

Development (IMPLAD), Beijing in 2010

DNA barcodes evaluation based on a large datasetDNA barcodes evaluation based on a large dataset

A total of 6286 individuals of 1757 species (1675 angiosperms and 82 gymnosperms) were used for universality analyses, and a total of 21673 sequences were obtained (5826 for rbcL, 5471 for matK, 5566 for trnH-psbA, and 4810 for ITS);

ITS was suggested to be part of the core barcode for plants

Community phylogeney of Dianhushan 20 ha forest dynamic plot with DNA barcoding

More accurate community phylogenetic tree with DNA barcodes,

To reveal the real coexistent and assemblage mechanism of

community. Pei et al. 2011. PLoS One 6: e21273

183 speciesrbcL, trnH-psbA, and matK：1–2 individuals

Phylogenetic clustering : valley and low-slope habitatsPhylogenetic over-dispersed: high-slope, ridge-top and high-gully habitats

13 articles of plant DNA barcoding published in this special issue;

All of them were supported by the grant from the Large-Scale Scientific Facilities of the Chinese Academy of Sciences (No. 2009-LSF-GBOWS-01).

Other projects

Animal DNA Barcodes and Cryptic Diversity, an NSFC key-note

project (10 million CN Yuan, Jan.2011 – Dec. 2014), PIs: Ya-Ping

Zhang, Da-Wei Huang, Jing Che and Shun-Ping He

Technical Standards and Information System for DNA barcoding in

China (10 million CN Yuan, July. 2011 – June 2015), PI: Ge-Xia Qiao

DNA barcoding in Some Natural Reserves (10 million CN Yuan, July

2012 – June 2016), PI: Douglas Yu

Digitalization of the Flora Reipublicae Popularis Sinicae and DNA

barcoding (10 million CN Yuan, July 2013 – June 2017), Pis: Xian-

Chun Zhang & Hong Wang

Barriers and threats DNA barcoding is becoming a fashion in many areas

in China but some do not understand the principles

of DNA barcoding

China is a centralized country but with dicentralized

funding and different funding agencies have different

criteria

Lack of qualified taxonomists and many young

students play DNA barcoding without a proper

training in taxonomy

Problems in getting data from other parties

China signs iBOL MOU to consolidate its

central node status 31 May 2012

The 5th International Barcode of Life Conference Kunming, China

27- 31 Oct. 2013

http://www.dnabarcodes2013.org

Call for papers, symposium and

workshop proposal

Thank You！Thank You！