CHINA LISTED

LIGHTENING

TESTED SKIN-LIGHTENING EFFECT FROM PLANT EXTRACTS

lightening activity.

lightening

5

4. Saija A, et al., in vitro antioxidant activity and in vivo photoprotective effect of a red orange extract. Int J Cosmet Sci 20: 331-342 (1998).

lightening

Opuntia ficus-indica stem extract

1. Di Lorenzo et al., The polysaccharide and low molecular weight components of Opuntia ficus indica cladodes: Structure and skin repairing properties.

Carbohydrate Polymers 157 (2017) 128–1362. Bionap report. Opuntia ficus indica extract in skin hydrationand repair.

OPUNTIA BIOCOMPLEX SH, contains compounds with different chemical nature and molecular weight (MW) distribution.The high MW components of Opuntia mucilage have been identified as a linear galactan polymer and a highly branched xyloarabinan. The low MW components have been attributed to lactic acid, mannitol and phenolic compounds such as piscid acid and eucomic acid (1). Skin-repairing and hydrating effects of OPUNTIA BIOCOMPLEX SH have been proven by in vitro model and clinical evaluation (1,2). A new in vitro model (Time Lapse Microscopy) on keratinocyte cells was used to evaluate the effect of OPUNTIA BIOCOMPLEX SH on skin repair and protection, simulating in vivo conditions (1). Results have proven that Opuntia cladode extract induced a wound healing effect (WH) of 80% at 20 h, with a total wound closure within 30 h, whereas a significantly higher healing time was required in the control for the same effect (80% WH at 28 h and total closure at 50 h). The skin-moisturizing effect of OPUNTIA BIOCOMPLEX SH has been evaluated in a randomized, double blind and placebo controlled clinical trial, carried out on 40 healthy subjects (2). The topical effect of OPUNTIA BIOCOMPLEX SH at 2% w/w on skin hydration was evaluated by corneometer over a period of 30 days and at short and long-term time points (1 hour, 15 days and 30 days). Results have showed that OPUNTIA BIOCOMPLEX SH is able to induce a significant increase of water content on skin in a short and long- term applications. In detail, 11.4%, 25.3% and 24.8% of change of skin hydration was respectively recorded at 1 h (short term), 15 days and 30 days (long term) for OPUNTIA BIOCOMPLEX SH at 2%, whereas 8.51%, 7.73% and 8.84% was observed in the control (Fig.1).

Moreover, data obtained from the Visioscan technique have confirmed the moisturizing action. SEsc (scaliness) and SEsm (smoothness) parameters showed a significant improvement vs placebo after 15 days of treatment. OPUNTIA BIOCOMPLEX SH reduced the scaliness of more than 18% vs 4,9% of placebo and increased smoothness of more than 7% vs 1,7% of placebo. Finally, cutometer investigations on R0 (firmness), R6 (visco-elasticity) and R7 (biological elasticity) parameters have suggested that the activity of OPUNTIA BIOCOMPLEX SH can be attributed to increase in water content, not only in the superficial, but also in deeper skin layers. The Opuntia extract, promoting skin hydration, counteracts the superficial skin flaking and dryness, and leads to a significant improvement of skin smoothness.

Fig 1 Corneometric measurement: percentage change variation of skin water content vs baseline in subjects treated with formulation containing tested ingredient (Opuntia Biocomplex SH 2% w/w) or not (Control).

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Opuntia Biocomplex SH Control

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Opuntia ficus-indica stem extract

1-4

4. Bonina F, et al., Biofenoli dell'ulivo: attività antiradicalica e fotoprotettiva in vivo dell'oleuropeina e dell'idrossitirosolo. Cosmetic Technology, 2:18-22 (1999)

Bionap report. Photo-protective effects of OLEA- HT 10

(2013).

Salucci S., et al., Antioxidants in the prevention of UVB-inducedkeratinocyte apoptosis. J of Photochemistry and Photobiology B: Biology 141, 1-9 (2014)

Salucci S., et al., Tyrosol prevents apoptosis in irradiated kera-tinocytes. Journal of Dermatological Science 80:61-68 (2015).Bonina F., et al., Biofenoli dell'ulivo: attività antiradicalica e foto-rotettiva in vivo dell'oleuropeina e dell'idrossitirosolo.

Cosmetic Technology, 2:18-22 (1999).p

According to Bionap’s phylosophy, OLEA-HT 10 is obtained from a by-product of the sicilian olive oil production. The ingredient is a light-colored powder containing hydroxytyrosol and tyrosol (10-12% HPLC).

What it does• Several in vitro and clinical studies have proved the radical scavenging and antioxidant activity of hydroxytyrosol and tyrosol (1-6). These activities are impor-tant to protect proteins such as collagen and elastin that influence skin elasticity and turgor.• OLEA-HT 10 is able to counteract skin photoaging signs (wrinkles, hyperpigmen-tation, skin sensitivity) and UV-induced DNA damage.

How to useOLEA-HT 10 is a key ingredient for face and body formulations (emulsions, lotions, gels, cleaners, etc) specially aimed at preventing photo-aging and skin spots.

OLEA-HT 10 %(w/w)

10-12Hydroxytyrosol and tyrosol

(HPLC method)

Bionap report. Photo-protective effects of OLEA- HT 10

(2013).

Salucci S., et al., Antioxidants in the prevention of UVB-inducedkeratinocyte apoptosis. J of Photochemistry and Photobiology B: Biology 141, 1-9 (2014)

Salucci S., et al., Tyrosol prevents apoptosis in irradiated kera-tinocytes. Journal of Dermatological Science 80:61-68 (2015).Bonina F., et al., Biofenoli dell'ulivo: attività antiradicalica e foto-rotettiva in vivo dell'oleuropeina e dell'idrossitirosolo.

Cosmetic Technology, 2:18-22 (1999).p

According to Bionap’s phylosophy, OLEA-HT 10 is obtained from a by-product of the sicilian olive oil production. The ingredient is a light-colored powder containing hydroxytyrosol and tyrosol (10-12% HPLC).

What it does• Several in vitro and clinical studies have proved the radical scavenging and antioxidant activity of hydroxytyrosol and tyrosol (1-6). These activities are impor-tant to protect proteins such as collagen and elastin that influence skin elasticity and turgor.• OLEA-HT 10 is able to counteract skin photoaging signs (wrinkles, hyperpigmen-tation, skin sensitivity) and UV-induced DNA damage.

How to useOLEA-HT 10 is a key ingredient for face and body formulations (emulsions, lotions, gels, cleaners, etc) specially aimed at preventing photo-aging and skin spots.

OLEA-HT 10 %(w/w)

10-12Hydroxytyrosol and tyrosol

(HPLC method)

1. Rizza L. et al., Comparative in vivo study of the efficacy and tolerance of exfoliating agents using reflectance spectrophotometric methods. J Cosmet Sci. 61(3):247-58 (2010).

2. Babilas P., et al., Cosmetic and dermatologic use of alpha hydroxy acids. JDDG. 10: 488-491 (2012).

sicilian white grapes

1. Rizza L. et al., Comparative in vivo study of the efficacy and tolerance of exfoliating agents using reflectance spectrophotometric methods. J Cosmet Sci. 61(3):247-58 (2010).

2. Babilas P., et al., Cosmetic and dermatologic use of alpha hydroxy acids. JDDG. 10: 488-491 (2012).

(1-3)

Bionap report. Effect of bergamot flavonoids extract on adipocytes cells (2014).

levels in mesenchymal stem cells from human adipose tissue. PPAR Research, vol. 2016 - 9pages (2016)

2. Lo Furno D., et al., A Citrus bergamia extract decreases adipogenesis and increases lipolysis by modulating PPAR

(1-3)

Bionap report. Effect of bergamot flavonoids extract on adipocytes cells (2014).

levels in mesenchymal stem cells from human adipose tissue. PPAR Research, vol. 2016 - 9pages (2016)

2. Lo Furno D., et al., A Citrus bergamia extract decreases adipogenesis and increases lipolysis by modulating PPAR

2

Opuntia ficus-indica stem extract

2

1. Compositions for the treatment of hemorrhoids and related diseases - PCT/EP2010/005346 (patent).

2. Rizza L,et al., Caco-2 cell line as a model to evaluate mucoprotective properties. Int J Pharm 422 (1-2): 318-322 (2012).

REV. MAR 2017