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1
Inferential StatisticsInferential StatisticsDetails of t-test & chi-
square test
2
INTRODUCTION
T-TEST- Numerical Data- Comparing the Mean of
two groups
CHI-SQUARE- Categorical Data
BOTH TESTS ARE USED FOR UNPAIRED OBSERVATION
3
T - TEST
Also referred as Student t-testSuppose we have taken a sample of 100 women and
found mean Hb as 11.4 gm. Now the question is whether this sample is from a population whose
standard deviation is 2 gm and mean Hb is 12 or not?
What should we be looking at? Is there a difference b/w
population mean and sample mean?
In this case difference is there Again the question is; Is this
difference significant ? Or it is due to chance alone
4
Of course the difference should be large enough to be called significant
Now the difference can be written as Sample Mean – population mean
What other factor can effect the importance of the difference ?
If there is great variation in the population (Standard deviation with small sample size) the difference would not be of that much importance
If there is small variation (standard deviation with large sample size) a slight degree of difference can become important
5
So we should add this factor as well to
decide about the importance of difference
The formula becomes Sample Mean – Population
mean Standard deviation/√N
And this is given the name of t test
6
Hence the t test =
T= Sample Mean – population mean
Standard deviation/√N Putting the values in the formula t = 11.4 -12 = -0.6 2/√100 2/10
Calculated value of t = - 0.6/0.2 = - 3.0Critical value (cut off) = ± 2.00What should be the conclusionReject Ho or not As the calculated value is more than critical we
reject chance explanation and conclude that the sample is not from a population whose mean Hb level is 12 gm.
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Example 2 :In a certain province the proportion of women who are delivered through caesarian section is very high. A study is, therefore, conducted to discover why this is the case. As small height is known to be one of the risk factors related to difficult deliveries, the researcher may want to find out if there is a difference between the mean height of women in this province who had normal deliveries and of those who had Caesarian sections. No. of women
in study Height in cms
Standard deviation
Normal delivery
60 156 3.1
CS 52 154 2.8
1. Calculating the T-Value2. Using a T-Table3. Interpreting the Result
8
USING THE T-TABLETo determine if null hypothesis is
rejected or not Significance level (p-value) - 0.05 Degree of freedom
- measure derived form the sample size
- student t-test the number of degree of freedom is calculated as the sum of two sample sizes minus 2
d.f. = 60 + 52 - 2 = 110
t-value belonging to the p-value and the degree of freedom is located in the table- t-value belonging to p -0.05 and d.f. = 120 and we find it is 1.98
9
INTERPRETING THE RESULT Decision Rule Calculated t-value is larger than the value
derived from the table. p is smaller than the value indicated at the top of the column. We than reject the null hypothesis and conclude a statistical significant difference b/w the two means.
Calculated t-value is smaller, p is larger than the value indicated, we accept null hypothesis and conclude observed difference is not statistically significance.
10
INTERPRETING THE RESULT
t-value calculated in step 1 is 3.6 larger than t-value derived in step 2 is (1.98)
p is smaller than 0.05, we reject the null hypothesis
Observed difference of two cms b/w mean heights of women with normal del. and CS is statistically significant difference.
11
CHI-SQUARE (x2) TESTSuppose you are presented with two
vaccines A and B for the prevention of measles. Both the manufacturer claim that their vaccine is better. How would you resolve this issue?
If we want to decide the credibility of vaccine objectively. You would ask both the manufacture to provide some vaccine to conduct a clinical trial. 100 randomly selected children were given vaccine A and same number of children were vaccinated with B. Out of those vaccinated by A fifteen (15) developed infection 20 children vaccinated by B developed infection.
Which vaccine is better?
12
If Vaccine A Then Producer of vaccine B may claim that this difference is by chance.
However the producer of vaccine A will call this difference as significant.
The dispute will not be resolved even if the difference is large enough until a cut off value is decided by some neutral person and that neutral umpire is test of significance.
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By putting information from the data following 2x2 table can be filled
Type of vaccine Infection No infection Total
A 100
B 100
Total 200
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By putting information from the data following 2x2 table can be filled
Type of vaccine Infection No infection Total
A 15 100
B 20 100
Total 200
15
By putting information from the data following 2x2 table can be filled
Type of vaccine Infection No infection Total
A 15 85 100
B 20 80 100
Total 200
16
By putting information from the data following 2x2 table can be filled
Type of vaccine Infection No infection Total
A 15 85 100
B 20 80 100
Total 35 165 200
17
According to null Hypothesis If both vaccine had same effect then we should expect the same number of infection in both the groups. Accordingly the following table shows the shape of the table we should expect if null hypothesis is true
18
If both vaccine same then half of infection will be in vaccine A and Half in vaccine B and the table will look like this
Type of vaccine Infection No infection Total
A 100
B 100
Total 35 165 200
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Alternatively we can determine the expected value in cell a by the principle of unit. i.e.Out of 200 the number of infection are 35Out of 1 the number of infection will be 35/200Out of 100 the number of infection will be 35 x 100 = 17.5 200
Type of vaccine Infection No infection Total
A 17.5 100
B 100
Total 35 165 200
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If you look care fully then you will observe that in the first step we have divided the coloumn total of cell a with grand total and then multiplied it with row total of cell a. So this two step procedure for expected value can be written as a single step
Expected value(EV) of cell a = Column total x Row total
Grand Total
Thus we can use this formula to calculate expected value of any cell
Now we fill the table with all expected values
21
According to null hypothesis we should not find any difference in observed value in cells a, b, c, and d. and expected value. Here we find some difference. This difference may be small (insignificant) or large (significant). Hence the next logical step should be that we should subtract expected value from observed value to find the difference. We would get a table like this
Type of vaccine Infection No infection Total
A 17.5 82.5 100
B 17.5 82.5 100
Total 35 165 200
Cell Observed
Value
O
Expected
Value
E
O – E (O – E)2 (O – E)2/ E Total
a 15
b 85
c 20
d 80
Total
Prof. Dr. M. I. Siddiqui – Clinision Workshop
Cell Observed
Value
O
Expected
Value
E
O – E (O – E)2 (O – E)2/ E Total
a 15 17.5
b 85 82.5
c 20 17.5
d 80 82.5
Total
Prof. Dr. M. I. Siddiqui – Clinision Workshop
Cell Observed
Value
O
Expected
Value
E
O – E (O – E)2 (O – E)2/ E Total
a 15 17.5 -2.5
b 85 82.5 2.5
c 20 17.5 2.5
d 80 82.5 -2.5
Total
Prof. Dr. M. I. Siddiqui – Clinision Workshop
Cell Observed
Value
O
Expected
Value
E
O – E (O – E)2 (O – E)2/ E Total
a 15 17.5 -2.5 6.25
b 85 82.5 2.5 6.25
c 20 17.5 2.5 6.25
d 80 82.5 -2.5 6.25
Total
Prof. Dr. M. I. Siddiqui – Clinision Workshop
Cell Observed
Value
O
Expected
Value
E
O – E (O – E)2 (O – E)2/ E Total
a 15 17.5 -2.5 6.25 6.25/ 17.5
b 85 82.5 2.5 6.25 6.25/ 82.5
c 20 17.5 2.5 6.25 6.25/ 17.5
d 80 82.5 -2.5 6.25 6.25/82.5
Total
Prof. Dr. M. I. Siddiqui – Clinision Workshop
Cell Observed
Value
O
Expected
Value
E
O – E (O – E)2 (O – E)2/ E Total
a 15 17.5 -2.5 6.25 6.25/ 17.5 0.36
b 85 82.5 2.5 6.25 6.25/ 82.5 0.07
c 20 17.5 2.5 6.25 6.25/ 17.5 0.36
d 80 82.5 -2.5 6.25 6.25/82.5 0.07
Total = 0.86
Prof. Dr. M. I. Siddiqui – Clinision Workshop
28
Table 4.12
D.F .0.50 .10 .050. 020 .010 .0030 .001
1 0.45 2.74 3.84 5.41 6.04 7.68 10.832 1.39 4.61 5.99 7.82 9.21 10 13.623 2.37 6.25 7.82 9.84 11.34 12.84 16.274 3.36 7.78 9.49 11.67 13.28 14.36 18.475 4.35 9.24 11.07 13.39 15.09 16.75 20.316 5.35 10.65 12.59 15.03 16.81 18.55 22.467 6.35 12.02 14.07 10.62 18.48 20.28 24.328 7.34 13.36 15.51 18.17 20.09 21.96 26.139 8.34 14.68 16.92 19.68 21.67 23.59 27.8810 9.34 15.99 18.31 21.16 23.21 25.19 29.59
( Source. JE Park K. Park, Text Book of Preventive and social Medicine)
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Calculated value of difference = 0.86
Critical value = 3.84 from the table of Chi
square at 5% level of significance
Decision ?
As the difference is less than the minimum difference
to be called significant. We conclude that there is
no significant difference between two vaccine and
we fail to reject null hypothesis.
In simple English both the vaccine are of same
efficacy and the difference is by chance and we
can decide to buy the one which is cheaper.
30
CHI-SQUARE (x2) TESTExample:
Suppose that a study of the factors affecting the utilization of antenatal clinics you found that 51 women out of 80 who lived within 10 km of the clinic came for antenatal care, compared to only 35 out of 75 of those who lived more than 10 km away. This suggests that antenatal care (ANC) is used more often by women who live close to the clinics.
Distance from ANC Used ANC Did not use ANC Total
Less than 10 km 51 (64%) 29 (36%) 80 (100%)
10 km or > 35 (47%) 40(53%) 75 (100%)
Total 86 69 155
1) Calculate the x2 value2) Using a x2 Table3) Interpreting the Result
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Distance from ANC
Used ANC
Did not use ANC
Total
Less than 10 km
80 (100%)
10 km or > 75 (100%)
Total 86 69 155
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By putting expected value of cell from our calculation
Distance from ANC
Used ANC
Did not use ANC
Total
Less than 10 km
44.4 35.6 80 (100%)
10 km or > 41.6 33.4 75 (100%)
Total 86 69 155
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If there are 155 user then the ANC was used by 86
If there was 1 user then ANC was used by 86/155
If there were 80 people then the ANC was used by 80X86/155 (equation 1)
44.4We can write this expression as 80 = row total (RT)86 = coloumn Total (CT)155 = Grand total (GT)So we can write equation one as follow
EV= RTXCT/GTHence we can calculate expected value of
any cell with this expression or formula
34
CALCULATE X2 VALUE
Expected frequency (E) for each cell.E = row total x column total / grand (overall) total
Each cell, subtract the expected frequency from the observed frequency (O)
O - E For each cell square the result of (O-E)
and divide by expected frequency E. Add the result of the above step for all
the cells
Cont…
35
CALCULATE X2 VALUE Formula for calculating chi-
square value:x2 = ( 0 - E)2 / E
O is the observe frequency (indicated in the table)E is the expected frequency to be calculated (the sum of) directs you to add together the products
of (O-E)2 for all the cell of the table
For two by two table (which contain 4 cells) the formula isx2 = [(01 - E1)2 / E1] + [(02 - E2)2 / E2] + [(03 -
E3)2 / E3] + [(04 - E4)2 / E4]
36
USING X2 TABLE
Decide a p-value example 0.05 Degree of freedom
df = (r-1) x (c-1)
for a 2 by 2 table the no. of d.f. is 1 (i.e. d.f. = (2-1) x (2-1) = 1)
37
INTERPRETING THE RESULT
Step 1 (a)
expected frequency for each cell
E1 = 86 x 80 / 155 = 44.4Distance from ANC Used ANC Did not use ANC Total
Less than 10 km O1 = 51 E1 = 44.44 O2 = 29 E2 = 35.6 80
10 km or > O3 = 35 E3 = 41.6 O4 = 40 E4 = 33.4 75
Total 86 69 155
null hypothesis is rejected if < 0.05 which is the case if x2 is larger than theoretical x2 in table
Cont…
38
INTERPRETING THE RESULT
Step 1(b) to (1d)
x2 = (51- 44.4)2 / 44.4 + …… + …… = 0.98 + 1.22 + 1.05 + 1.30 = 4.55
Step 2- (d.f.) is 1
- table of chi-square decided p-value = 0.05
- d.f. is 1, we look along row in the column where p=0.05. This gives us value of 3.84. Our value of 4.55 is > 3.84, which means that the p-value is < 0.05
Contd..
39
INTERPRETING THE RESULT
Step 3
We can now conclude that the women living within the distance of 10 km from the clinic used antenatal care significantly more often than women living more than 10 km away.
40
INTERPRETATION OF EXAMPLE
64% of the women living within a distance of 10 km from the clinic used antenatal care during pregnancy, compared to only 47% of women living 10 km or further away from the nearest clinic. This difference is statistically significant (x2 = 4.55; - < 0.05)
41
NOTE• x2 test applied if the sample is large enough
• General rule total should be at least 40 and the expected frequency in each of the cells should be at least 5.
• x2 test can be used to compare more than two groups
• A table with 3 or more rows or columns would be designed rather than a two by two tables
• In above example < 5 km, 5 - 10 km, > 10 km data would be put in 2 x 3 tables.
• d.f. would be (3 -1) x (2 - 1) = 2
42
QUICK FORMULA For two-by-two tables there is a quick method for
calculating the chi-square value, which can replace step 1 described above.
If the various numbers in the costs table are represented by the following letters
The quick formula for calculating the Chi-square value is
x2 = n (ad - bc)2 / efgh
Condition Total
+ -
Exposure a b e
Yes c d f
No g h n
43
Objectives
By the end of session the participant will be able to
Define test of significance
Name test of significance according to type of data
Able to calculate T test
Able to compute X2
Interpret the result
Interpret statistical results in simple English language
TutorialExercise for test of significanceA surgeon wants to compare the two surgical procedure for hysterectomy, Abdominal Hysterectomy (AH) and Vaginal Hysterectomy (VH) with a research question of infection rate between two surgical procedures. She operates on 400 patient with VH approach and 40 developed infection. Out of 100 AH cases 20 developed infection
Answer the following questions
a) What should be the title of research for publication in a journal?
b) Frame Null hypothesis in this case?c) What level of significance you will set?.d) What test of significance you will apply and why? e) Calculate test statisticsf) Interpret your result in statistical languageg) Describe the result in simple English
45
Home Assignment
Home Assignment
Do exercise 12.1, 12.2 , 12.3 12.10, 12.13, 12.19