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Chapter 7
Membrane Structure and Function
• In 1935, Hugh Davson and James Danielli proposed a sandwich model in which the phospholipid bilayer lies between two layers of globular proteins
• Later studies found problems with this model, particularly the placement of membrane proteins, which have hydrophilic and hydrophobic regions
• In 1972, J. Singer and G. Nicolson proposed that the membrane is a mosaic of proteins dispersed within the bilayer, with only the hydrophilic regions exposed to water
Fig. 7-2
Hydrophilichead
WATER
Hydrophobictail
WATER
amphipathic molecules
Fig. 7-3
Phospholipidbilayer
Hydrophobic regionsof protein
Hydrophilicregions of protein
Fig. 7-4
TECHNIQUE
Extracellularlayer
KnifeProteins Inside of extracellular layer
RESULTS
Inside of cytoplasmic layer
Cytoplasmic layerPlasma membrane
Freeze fracture
Fig. 7-5
Lateral movement(~107 times per second)
Flip-flop(~ once per month)
(a) Movement of phospholipids
(b) Membrane fluidity
Fluid Viscous
Unsaturated hydrocarbontails with kinks
Saturated hydro-carbon tails
(c) Cholesterol within the animal cell membrane
Cholesterol
The fluidity of membrane
• The steroid cholesterol has different effects on membrane fluidity at different temperatures
• At warm temperatures (such as 37°C), cholesterol restrains movement of phospholipids
• At cool temperatures, it maintains fluidity by preventing tight packing
Cholesterol
Fig. 7-6
RESULTS
Membrane proteins
Mouse cellHuman cell
Hybrid cell
Mixed proteinsafter 1 hour
Fig. 7-7
Fibers ofextracellularmatrix (ECM)
Glyco-protein
Microfilamentsof cytoskeleton
Cholesterol
Peripheralproteins
Integralprotein
CYTOPLASMIC SIDEOF MEMBRANE
GlycolipidEXTRACELLULARSIDE OFMEMBRANE
Carbohydrate
Fig. 7-8
N-terminus
C-terminus
HelixCYTOPLASMICSIDE
EXTRACELLULARSIDE
Fig. 7-9
(a) Transport
ATP
(b) Enzymatic activity
Enzymes
(c) Signal transduction
Signal transduction
Signaling molecule
Receptor
(d) Cell-cell recognition
Glyco-protein
(e) Intercellular joining (f) Attachment to the cytoskeleton and extracellular matrix (ECM)
•Six major functions of membrane proteins:
Synthesis and sideness of membranes
Fig. 7-10
ER1
Transmembraneglycoproteins
Secretoryprotein
Glycolipid
2Golgiapparatus
Vesicle
3
4
Secretedprotein
Transmembraneglycoprotein
Plasma membrane:
Cytoplasmic face
Extracellular face
Membrane glycolipid
• Concept 7.2: Membrane structure results in selective permeability
Fig. 7-11Molecules of dye Membrane (cross section)
WATER
Net diffusion Net diffusion Equilibrium
(a) Diffusion of one solute
Net diffusion
Net diffusion
Net diffusion
Net diffusion
Equilibrium
Equilibrium
(b) Diffusion of two solutes
Lowerconcentrationof solute (sugar)
Fig. 7-12
H2O
Higher concentrationof sugar
Selectivelypermeablemembrane
Same concentrationof sugar
Osmosis
Osmosis is the diffusion of water
across a selectively permeable membrane
Hypotonic solution
(a) Animal cell
(b) Plant
cell
H2O
Lysed
H2O
Turgid (normal)
H2O
H2O
H2O
H2O
Normal
Isotonic solution
Flaccid
H2O
H2O
Shriveled
Plasmolyzed
Hypertonic solution
Fig. 7-13
Tonicity is the ability of a solution to cause a cell to gain or lose water
Fig. 7-14 Osmoregulation
Filling vacuole 50 µm
(a) A contractile vacuole fills with fluid that enters from a system of canals radiating throughout the cytoplasm.
Contracting vacuole
(b) When full, the vacuole and canals contract, expelling fluid from the cell.
Fig. 7-15
EXTRACELLULAR FLUID
Channel protein
(a) A channel protein
Solute CYTOPLASM
Solute Carrier protein
(b) A carrier protein (Shape change)
Some diseases are caused by malfunctions in specific transport systems, for example the
kidney disease cystinuria胱胺酸尿症 (#)
• Cystinuria is characterized by the inadequate reabsorption of cystine during the filtering process in the kidneys, thus resulting in an excessive concentration of this amino acid. Cystine may precipitate out of the urine, if the urine is neutral or acidic, and form crystals or stones in the kidneys, ureters, or bladder.
• Cystine?
2
Fig. 7-16-7 Active transportEXTRACELLULAR
FLUID [Na+] high [K+] low
[Na+] low
[K+] high
Na+
Na+
Na+
Na+
Na+
Na+
CYTOPLASM ATP
ADP P
Na+ Na+
Na+
P 3
K+
K+ 6
K+
K+
5 4
K+
K+
P P
1(Phosphoryla
tion)
(dephosphorylation)
Fig. 7-17Passive transport
Diffusion Facilitated diffusion
Active transport
ATP
How Ion Pumps Maintain Membrane Potential
• Membrane potential is the voltage difference across a membrane
• Voltage is created by differences in the distribution of positive and negative ions
• Two combined forces, collectively called the electrochemical gradient, drive the diffusion of ions across a membrane:– A chemical force (the ion’s concentration gradient)
– An electrical force (the effect of the membrane potential on the ion’s movement)
•An electrogenic pump is a transport protein that generates voltage across a membrane•The sodium-potassium pump is the major electrogenic pump of animal cells
• The main electrogenic pump of plants, fungi, and bacteria is a proton pump
Fig. 7-18
EXTRACELLULARFLUID
H+
H+
H+
H+
Proton pump
+
+
+
H+
H+
+
+
H+
–
–
–
–
ATP
CYTOPLASM
–
Cotransport: Coupled Transport by a Membrane Protein
• Cotransport occurs when active transport of a solute indirectly drives transport of another solute
• Plants commonly use the gradient of hydrogen ions generated by proton pumps to drive active transport of nutrients into the cell
Fig. 7-19
Proton pump
–
–
–
–
–
–
+
+
+
+
+
+
ATP
H+
H+
H+
H+
H+
H+
H+
H+
Diffusionof H+
Sucrose-H+
cotransporter
Sucrose
Sucrose
Concept 7.5: Bulk transport across the plasma membrane occurs by exocytosis and
endocytosis• In exocytosis, transport vesicles migrate to the membrane, fuse with it, and release their contents– Many secretory cells use exocytosis to export their products
• In endocytosis, the cell takes in macromolecules by forming vesicles from the plasma membrane
– There are three types of endocytosis:– Phagocytosis (“cellular eating”)– Pinocytosis (“cellular drinking”)– Receptor-mediated endocytosis
Fig. 7-20PHAGOCYTOSIS
EXTRACELLULARFLUID
CYTOPLASM
Pseudopodium
“Food”orother particle
Foodvacuole
PINOCYTOSIS
1 µm
Pseudopodiumof amoeba
Bacterium
Food vacuole
An amoeba engulfing a bacteriumvia phagocytosis (TEM)
Plasmamembrane
Vesicle
0.5 µm
Pinocytosis vesiclesforming (arrows) ina cell lining a smallblood vessel (TEM)
RECEPTOR-MEDIATED ENDOCYTOSIS
Receptor Coat protein
Coatedvesicle
Coatedpit
Ligand
Coatprotein
Plasmamembrane
A coated pitand a coatedvesicle formedduringreceptor-mediatedendocytosis(TEMs)
0.25 µm
Fig. 7-UN3
Environment:0.01 M sucrose
0.01 M glucose
0.01 M fructose
“Cell”
0.03 M sucrose
0.02 M glucose
Fig. 7-UN4
You should now be able to:1. Define the following terms: amphipathic
molecules, aquaporins, diffusion2. Explain how membrane fluidity is
influenced by temperature and membrane composition
3. Distinguish between the following pairs or sets of terms: peripheral and integral membrane proteins; channel and carrier proteins; osmosis, facilitated diffusion, and active transport; hypertonic, hypotonic, and isotonic solutions
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
4. Explain how transport proteins facilitate diffusion
5. Explain how an electrogenic pump creates voltage across a membrane, and name two electrogenic pumps
6. Explain how large molecules are transported across a cell membrane
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings