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Chapter 15 Genetic Engineering

Chapter 15 Genetic Engineering. Chapter Mystery A case of mistaken identity Page 417 Hypothesis: How did the police know they had the wrong suspect?

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Chapter 15 Genetic Engineering Slide 2 Chapter Mystery A case of mistaken identity Page 417 Hypothesis: How did the police know they had the wrong suspect? Slide 3 Section 15.1 Selective Breeding Objectives: What is selective breeding used for ? How do people increase genetic variation? Define: Selective breeding Hybridization Inbreeding biotechnology Slide 4 I. Selective Breeding Dogs = Chihuahua Great Dane Humans bred dogs for 1000s of years looking to produce better hunters, retrievers, companions Selective breeding allowing only those animals with wanted characteristics to produce the next generation Humans use selective breeding, which takes advantage of naturally occurring genetic variation, to pass wanted traits on to the next generation of organisms Produced new varieties of cultivated plants and all domestic animals (horses, cats, cows) by selectively breeding for particular traits Native Americans selectively bred teosinte (wild grass) to produce corn = more nutritious and productive Slide 5 A. Hybridization Luther Burbank (1849 1926) developed >800 varieties of plants Hybridization crossing dissimilar individuals to bring together best of both organisms Hybrids individuals produced by crosses Often hardier than either of parents Burbanks crosses combined disease resistance of one plant w/ food-producing capacity of another Results in new line of plants that had traits farmers needed to increase food production Slide 6 B. Inbreeding Inbreeding continued breeding of individuals with similar characteristics Used to maintain desirable characteristics in a line of organisms Helps ensure that characteristics that make each breed unique are preserved Can be risky Most members of a breed = genetically similar Increases chance that a cross b/w 2 individuals will bring together 2 recessive alleles for genetic defect Slide 7 II. Increasing Variation Breeders can increase the genetic variation in a population by introducing mutations, which are the ultimate source of biological diversity Biotechnology application of a technological process, invention, or method Used when manipulate genetic makeup of an organism Selective breeding = one form (important in agriculture and medicine) Slide 8 A. Bacterial Mutations Mutations heritable changes in DNA Occur spontaneously Can be increased by breeders by using radiation or chemicals Many harmful to organism With luck and perseverance breeders can produce a few mutants w/ useful characteristics not found in original population Useful in bacteria Small Millions can be treated w/ radiation/chemicals at same time = increases chance of producing a useful mutant Scientists have produced 100s of useful bacteria Some consume oil (oil spills) Working on bacteria that can clean up radioactive substances and metal pollution in environment Slide 9 B. Polyploid Plants Drugs that prevent separation of chromosomes during meiosis = useful in plant breeding Produce cells that have many times normal number of chromosomes Plants grown from these cells = polyploid b/c they have many sets of chromosomes Polyploidy usually fatal in animals; plants much better at tolerating extra sets of chromosomes Can quickly produce new species of plants: larger and stronger than diploid relatives Bananas, citrus fruits Slide 10 Section 15.2 Recombinant DNA Objectives: How do scientists copy the DNA of living organism? How is recombinant DNA used? How can genes from one organism be inserted into another organism? Define: Polymerase chain reaction Recombinant DNA Plasmid Genetic marker Transgenic Clone Slide 11 I. Copying DNA In the past mutations unpredictable Today genetic engineers can transfer certain genes at will from one organism to another = new living things DNA extracted from cells cut into fragments separated according to size find DNA of single gene among 3 million fragments!!!! Slide 12 Mystery Clue Page 421 How could restriction enzymes be used to analyze the DNA evidence found on the suspect? Slide 13 A. Finding Genes 1987 Douglas Prasher jellyfish wanted to find gene that codes for green fluorescent protein GFP absorbs energy from light & makes jellyfish parts glow Wanted to link GFP to when a protein was being made in a cell To find the gene using mRNA and radioactive probes to bind to complementary base sequences Slide 14 B. Polymerase Reaction Chain Heat piece of DNA separates strands As DNA cools, primer binds to single strands DNA polymerase starts copying region b/w primers Copies serve as templates to make more copies Slide 15 II. Changing DNA Wondered how to change DNA of living cell Griffith answered transformation: cell takes DNA from outside cell, added DNA becomes component of cells own genome Heat-killed bacteria contained DNA fragments When mixed w/ live bacteria some took up fragments transformed bacteria (changed characteristics Slide 16 A. Combining DNA Fragments Today can build custom DNA molecules with genes you like & insert them into living cells Recombinant DNA technology joining together DNA from 2 or more sources Makes it possible to change the genetic composition of living organisms Can investigate the structure and function of genes Slide 17 B. Plasmids and Genetic Markers Scientists join recombinant DNA to another piece of DNA containing a replication start signal whenever cell copies its own DNA, it copies recombinant DNA too Plasmid small circular DNA molecule Join DNA to plasmid use recombinant plasmid to transform bacteria results in replication of newly added DNA w/ rest of cells genome Genetic marker gene that makes it possible to distinguish bacteria that carry the plasmid from those that dont Used to locate transformed cells Slide 18 III. Transgenic Organisms Transgenic contain genes from other species Transgenic organisms can be produced by the insertion of recombinant DNA into the genome of a host organism 1980s perfected using mice Now plants, animals, microorganisms Contributes to understanding gene regulation and expression Slide 19 A. Transgenic Plants Slide 20 B. Transgenic Animals Slide 21 C. Cloning Clone member of a population of genetically identical cells produced from a single cell Use single cell from adult organism to grow an entirely new individual that is genetically identical to organism from which cell was taken 1952 1 st clone of animals amphibian tadpoles 1997 Ian Wilmut cloned sheep Dolly Since = cloned cows, pigs, mice, cats Slide 22 Section 15.3 Applications of Genetic Engineering Objectives: How can genetic engineering benefit agriculture and industry? How can recombinant-DNA technology improve human health? How is DNA used to identify individuals? Define: Gene therapy DNA microarray DNA fingerprinting forensics Slide 23 I. Agriculture and Industry Everything we eat and much of what we wear come from living organisms Use genetic engineering to try to improve products from plants and animals Ideally, genetic modification could lead to better, less expensive, and more nutritious food as well as less-harmful manufacturing processes. Slide 24 A. GM Crops Introduced 1996 2007 GM crops = 92% soybeans; 86% cotton; 80% corn grown in US Use bacterial genes that produce protein (Bt toxin) -Harmless to humans -Enzyme in insects converts Bt toxin into a form that kills the insect -No pesticides needed & produce higher yield of crops Resistance to herbicides (chemicals that destroy weeds) Resistance to viral infections Produce foods resistant to rot and spoilage (soon) Produce plastics for manufacturing industry (soon) Slide 25 B. GM Animals Food supply 30% milk from cows injected w/ hormones made by recombinant-DNA techniques to increase milk production Pigs produce more lean meat or high levels of healthy omega-3 acids Salmon growth hormone = grow more quickly (grown in captivity) Spider genes into goats = manufacture silk w/ milk extracted & woven into thread = light, tough, flexible material (military uniforms, medical sutures, tennis racket strings) Human genes + goat milk = antibacterial goat milk Hope to clone transgenic animals Increase food supply Save endangered species 2008 US govt approved sale of meat and milk from cloned animals Slide 26 II. Health and Medicine Biotechnology = part of medicine Early physicians extracted substances from plants and animals to cure patients 20 th century vaccination saved countless lives Today recombinant-DNA technology source of some of most important advances in prevention and treatment of disease Slide 27 A. Preventing Disease Golden rice incr. provitamin A + beta- carotene Hope to help prevent health problems (infant blindness) Transgenic plants/animals to make human antibodies Future transgenic animals may supply us w/ human proteins used in disease prevention Already produced in milk of some transgenic sheep and pigs Slide 28 B. Medical Research Transgenic animals used as test subjects Simulate human diseases caused by defective genes Use models based on simulations to follow onset and progression of diseases & test new drugs that may treat disease Ex: Alzheimers & arthritis Slide 29 C. Treating Disease Recombinant-DNA technology make important proteins that could prolong or save human lives Human growth hormone used to treat pituitary dwarfism now widely available b/c mass produced in recombinant bacteria Insulin treat diabetes Blood-clotting factors hemophilia Interleukin-2 & interferon cancer (future) Slide 30 Gene therapy process of changing a gene to treat a medical disease/disorder Absent or faulty gene is replaced by a normal, working gene Allows body to make the protein/enzyme needed eliminates cause of disorder Human Genome Project How it works: Engineer virus that cannot reproduce or cause harmful effects Put DNA w/ therapeutic gene into modified virus Infect patients cells w/ virus In theory: virus will insert healthy gene into target cell and correct defect Challenge: deliver gene that works correctly over long term High risk & experimental procedure To become accepted treatment need more reliable ways to insert working genes and ensure DNA used does no harm Slide 31 D. Genetic Testing Genetic tests use specific DNA sequences that detect complementary base sequences found in disease-causing alleles Other tests search for changes in cutting sites of restriction enzymes Some use tests to detect differences b/w length of normal and abnormal alleles Available for diagnosing hundreds of disorders Slide 32 E. Examining Active Genes All cells in human body contain identical genetic material Same genes not active in every cell DNA microarray technology used to study 100s/1000s of genes at once to understand levels of gene activity Slide 33 III. Personal Identification Complexity of human genome = no individual is exactly like any other genetically DNA fingerprinting analyzes sections of DNA that may have little or no function but that vary widely from one individual to another DNA samples from blood, sperm, tissue, hair w/ root Slide 34 A. Forensic Science Used since 1980s Precise & reliable Forensics scientific study of crime scene evidence DNA fingerprinting solve crimes, convict criminals, overturn wrongful convictions Wildlife conservation identify herds from which black-market ivory was taken (elephants in Africa) Slide 35 B. Establishing Relationships DNA fingerprinting disputed paternity: alleles in child not carried by mother must come from father Y chromosome never crosses over=few changes (father to child) miDNA (mitochondrial)- small so few changes (mother to child) Slide 36 Mystery Clue Page 434 What kind of evidence do you think investigators collected at the crime scene? What kinds of tests would they have to run on this evidence? What would the tests have to show before the suspect was released? Slide 37 Section 15.4 Ethics and Impacts of Biotechnology Objectives: What privacy issues does biotechnology raise? Are GM foods safe? Should genetic modifications to humans and other organisms be closely regulated? Assignment 2-column Chart & Essay: Write both viewpoints for each issue in this section to help you create a view point for each question above Write your opinion answer for each question above use support from the reading that is now in your chart Slide 38 I. Profits and Privacy Slide 39 II. Safety of Transgenics Slide 40 Mystery Clue Page 437 What privacy considerations, if any, should investigators have taken into account when obtaining the DNA evidence? Slide 41 III. Ethics of the New Biology Slide 42 Solve the Mystery Page 443 1.How did the investigators determine that the person they took into custody was not guilty of this crime? 2.Did the DNA evidence from the bloodstains come from the red blood cells, white blood cells, or both? EXPLAIN. 3.What if the initial suspect was related to the victim? Would that have changed the result? Why or why not? 4.What might have happened if this crime were committed before DNA fingerprinting was discovered? Describe the series of events that might have taken place after police took in the first suspect.