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Chaire en Biothérapie de l’Association Canadienne du Médicament Générique (ACMG) et Biosimilaires Canada

Davide Brambilla, PhD

November 23rd 2018

Davide Brambilla2018-11-21

Overview on 2017-2018

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The laboratory

Dr. Mahdi Roonikhan (Co-mentoring): Chemical Engineer, McGill Sam Babity: Chemist, ConcordiaElise Lazslo (Co-mentoring): Physico-Chemist, LorraineCarolina Penaloza: Biologist, Molecular biology, BogotàZineb Benkhachan: Pharmacy, Geneva2 summer students

www.brambillaudem.com

Fluorescence stereomicroscope, HPLC, Texture Analyser,Fluorescence plate reader, centrifuges, ovens, small equipment…

Overview 2017-2018

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Granted funding proposals:1. Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grants program (140 k$)2. Canada Foundation for Innovation (New Opportunities Fund) (302 k$)3. Réseau québécois de recherche sur les médicaments (RQRM) (Collaboration UdeM) (18 k$)4. Fonds de recherche du Québec - Société et culture (FRQSC) Audace (Collaboration, UdeM, Polytechnique, ICM) (100 k$)5. Fonds de recherche du Québec - Nature et technologies (FRQNT) New university researchers (96 k$)6. TransMedTech Project (Collaboration, UdeM, Polytechnique, ICM)

Funding proposals, under revision: 1. Canadian Institutes of Health Research (CIHR) (ULaval), Project Grant, (885 k$) 2. Alzheimer’s Society Canada (225 k$) 3. FRQS; Chercheur Bourier (200 k$) 4. SiliCycle Inc. (Collaboration 3 more profs) (308 k$)5. Research Tools and Instruments Grants Program, NSERC, (Collaboration 3 more profs) (138 k$)6. Bourse du Cercle du doyen (Faculté de pharmacie) (15 k$)

PDF fellowships:1 Alzhimer’s society2 TransMedTech1 FRQ-nt1 CIHR

PhD/Maitrise: 2 Faculté de Pharmacie1 Faculté de Pharmacie1 NSERC

Overview on 2017-2018

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Accepted publications: July 2017 – November 2018

1. Poly(Ethylene Glycol)-Alendronate Coated Nanoparticles for Magnetic Resonance Imaging of Lymph Nodes. Bisso S., DeGrassi A. Brambilla D. Leroux JC. J. DrugTargeting. 2018. Accepted.

2. Advances in the design of transdermal microneedles for diagnostic and monitoring applications. Babity S., Roohnikan M., Brambilla D. Small. 2018 Accepted.

3. 3D printing of pharmaceuticals – a disruptor or an enabler? Liang K., Brambilla D.*, Leroux JC*. *Co-corresponding authors. Adv. Mat., 2018. Accepted.

4. 3D printing of a wearable personalized oral delivery device: a first-in-human study. Liang K., Carmone S., Brambilla D., Leroux JC. Sci. Adv., 4 (5), eaat2544 2018.

5. Pharmacokinetics of Lipid-Drug Conjugates Loaded into Liposomes. Signorell D.R., Luciani P., Brambilla D. & Leroux JC. Eur. J. Pharm. Biopharm., 2018. 128, 188-199.

6. Antibody-Functionalized Polymer Nanoparticle Leading to Memory Recovery in Alzheimer's Disease-like Transgenic Mouse Model. Carradori D; Balducci C.; Brambilla D.; Re F.; Le Droumaguet B.; Flores O.; Gaudin A.; Mura S.; Forloni G.; Masserini M.; Nicolas J.*; Couvreur P.; Andrieux K.*; Nanomedicine:NBM. 2017, 14 (2), 609-618.

7. Poly(2-oxazoline)-Pterostilbene Block-Copolymer Nanoparticles for Dual-Anticancer Drug Delivery. Romio M., Morgese G., Trachsel L., Babity S., Paradisi C.,Brambilla D.*, Benetti EM.* Biomacromolecules. 2018 19 (1), 103-111. *Co-corresponding authors.

8. Drug Discovery, Development and Delivery in Alzheimer's Disease. Brambilla D. Pharm. Res. 2017, 35 (1), 3.

9. Preparation of PEGylated liposomes incorporating lipophilic lomeguatrib derivatives for the sensitization of chemo-resistant gliomas. Signorell, RD;Papachristodoulou, A.; Xiao, J.; Arpagaus B.; Casalini, T.; Grandjean, J.; Thamm, J.; Steiniger, F.; Luciani P.; Brambilla, D.; Werner, B.; Martin, E.; Weller, M.; Roth, P.;Leroux, JC. Int J Pharm. 2017, 536 (1), 388-396.

10. Sensitization of glioblastoma to alkylating chemotherapy by ultrasound-mediated delivery of therapeutic liposomes. P Roth, A Papachristodoulou, D Brambilla, RDSignorell, B Werner, Journal of Clinical Oncology 36, e14061-e14061

Overview on 2017-2018

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Editorial activity: Guest editor for Special Issue (Alzheimer’s disease) of Pharmaceutical Research. As of July 2018 allcontributors have been published

Peer-revision: Revision of over 15 original articles and reviews for international journals (J. Control Release, EJPB, Small,ACS Applied Nano Materials, Biomaterials, IJP)

Invited seminars: INTERNATIONAL PHARMACEUTICAL TECHNOLOGY SYMPOSIUM Sept. 17-19th 2018, Antalya, Turkey;International Seminar Series, Faculty of Medicine, June 5th 2018, University of Milano-Bicocca. Several presentation fromstudents or as co-author.

External event organization: representative of the CRS Canadian Chapter in the International Local Committee, CRS Canada Chapter,Chair of Biomaterials and drug delivery session CRS/CSPS joint meeting (21-24 May 2019, Vancouver).

Committe member: FRQ Excellence Fellowships selection, Expert reviewer Strategic Partnership Grants competition (NSERC)

Media highlights: Un embout buccal pour faciliter la prise de médicaments, LaPresse, RadioCanada

Projects directly supported by this Chaire

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Targeting NeutrophilExtracellular Traps

Transdermal peptide deliveryformulation

PJ 1 PJ 2

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The goal

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Project 1: Experimental Plan

1. Generate NET in the lab (Started)

2. Develop NET characterization protocol

3. NET targeted particles (flow condition)

4. DNA loaded particle

5. NET targeting and degradation studies (in vitro and in vivo)

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Project 2. Peptide delivery

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Chronic diseases,Daily s.c. adminsitration, 300 mg/kg

The platform

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People:Mahdi (PDF)Giorgio Sala (March 2019 – March 2020) MilanoGeneva (July – December 2019)

Study plan:- Develop analytical method (HPLC, LC-MS)- Design microneedles formulation- Test formulation in vitro (agarose gels)- Test formulation in vivo (bioavailability, PK)

Microneedles

Kim YC et al. Adv Drug Del Rev, 2012