USP <232>/<233>

Method Note Compendium

Updated April 6, 2018

ContentsMARSTM 6

Consumer Products

Suppository (Capsule) 2

Vaseline 3

Wax (Candle) 4

Nutraceuticals

Biotin 6

Ginko (Ground) 7

Ginko (Whole Pill) 8

Kelp 9

Whey Powder 10

Pharmaceutical & Biotech

(4-Fluorophenyl)diphenylsulfonium Triflate 12

Allergy Pill (Ground) 13

Allergy Pill (Whole Pill) 14

Antioxidant (Ground) 15

Antioxidant (Whole Pill) 16

APIs with Aromatic Ring Structures 17

Ascorbic Acid 18

Aspirin (Ground) 19

Aspirin (Whole) 20

Beta Carotene 21

Biotin 22

Calcium Carbonate 23

Calpan Pantothenic Acid 24

Cephalexin 25

Chromium Chelate 26

Cold and Flu Medicine (Liquid) 27

Cupric Sulfate 28

Diclofenac K 29

Empty Capsule (gel) 30

Fish Oil (No Capsule) 31

Folic Acid 32

Ginko (Ground) 33

Ginko (Whole Pill) 34

Kelp 35

Losataran K 36

Magnesium Carbonate 37

Magnesium Oxide 38

Manganese Carbonate 39

Metaformin (ground) 40

Multivitamin (Ground) 41

Multivitamin (Whole) 42

Niacinamide 43

Pantothenic Acid 44

Potassium Chelate 45

Pyridoxine 46

Riboflavin 47

Selenium Chelate 48

Stearic Acid 49

Thiamine 50

Trixie Phosphate in iPrep 51

USP 232/233 (Pharmaceuticals) 52

Vitamin B-12 53

Vitamin C (ground) 54

Vitamin C (whole) 55

Vitamin D 56

Vitamin D3 57

Vitamin E 58

Vitamins 59

Zinc Sulfate 60

Plastics, Polymers, & Oils

Mineral Oil 62

Discover® SP-D 80

Consumer Products

Suppository 64

Vaseline 65

Wax (candle) 66

Nutraceuticals

Ginko (Ground) 68

Ginko (Whole Pill) 69

Kelp 70

Pharmaceutical & Biotech

Allergy Pill (ground) 72

Allergy Pill (whole pill) 73

Antioxidant (Ground) 74

Antioxidant (whole pill) 75

Aspirin (Ground) 76

Aspirin (Whole) 77

Calpan Pantothenic Acid 78

Cephalexin 79

Chromium Chelate 80

Cold and Flu Medicine (liquid) 81

Diclofenac K 82

Empty Capsule 83

Fish Oil (No Capsule) 84

Folic Acid 85

Ginko (Ground) 86

Ginko (Whole Pill) 87

Kelp 88

Losataran K 89

Magnesium Oxide 90

Manganese Carbonate 91

Metaformin 92

Multivitamin (ground) 93

Multivitamin (whole) 94

Stearic Acid 95

Thiamine 96

USP 232/233 97

Vitamin C (ground) 98

Vitamin C (whole) 99

Vitamin D3 100

Zinc Sulfate 101

Consumer Products

MARS™ 6

2

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Suppository (Capsule)

Weigh 1 Capsule (approx. 1.0 g) into the digestion vessel. Add 12 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

Ensure that the capsule is completely covered with reagent before sealing the vessel.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

1 Capsule (approx 1 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofSuppository (Capsule)

3

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vaseline

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The use of HF may be required to digest any silicates found in the sample.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVaseline

4

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Wax (Candle)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofWax (Candle)

Nutraceuticals

MARS™ 6

6

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Biotin

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofBiotin

7

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Ginko (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofGinko (Ground)

8

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Ginko (Whole Pill)

Weigh 1 pill (approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3H2O2

1 pill (Approx 1.0 g) Organic Ramp to Temperature One Touch

210 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofGinko (Whole Pill)

9

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Kelp

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofKelp

10

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Whey Powder

Weigh 0.5 g of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.5 g Organic Ramp to Temperature One Touch

210 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofWhey Powder

Pharmaceutical & Biotech

MARS™ 6

12

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

(4-Fluorophenyl)diphenylsulfonium Triflate

Add 0.25 g of the sample into the digestion vessel. Add 9 mL of HNO3, and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

This application can only be run in the iPrep vessel.

This chemical compound is also know as Sudan Orange.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes.

MARS 6 iWave iPrep HNO3HCl

0.25 g Organic Ramp to Temperature One Touch

250 30:00 25:00 N/A 1800 Off

Sample was clear, gold colored, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion of(4-Fluorophenyl)diphenylsulfonium Triflate

13

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Allergy Pill (Ground)

Weigh 0.25 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.25 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAllergy Pill (Ground)

14

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Allergy Pill (Whole Pill)

Weigh 1 pill (Approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

H2 O2 (30%)

1 pill (Approx 1.0 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAllergy Pill (Whole Pill)

15

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Antioxidant (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAntioxidant (Ground)

16

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Antioxidant (Whole Pill)

Weigh 1 pill (approx 1.4 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

H2O2 (30%)

1 pill (Approx 1.4 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAntioxidant (Whole Pill)

17

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

APIs with Aromatic Ring Structures

Weigh 0.1 g - 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3 and 1 ml of HCl. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

This application can only be run in the iPrep vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6 iWave iPrep HNO3HCl

0.1 g - 0.5 g Organic Ramp to Temperature One Touch

250 30:00 25:00 N/A 1800 N/A

Sample was clear, colorless, and particle free upon dilution to 50 ml. If the sample contains precious metals the diluted sample may be the color associatedwith the metals.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAPIs with Aromatic Ring Structures

18

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Ascorbic Acid

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpress PlusXpress

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAscorbic Acid

19

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Aspirin (Ground)

Weigh 0.25 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.25 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAspirin (Ground)

20

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Aspirin (Whole)

Weigh 1 pill (Approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

1 pill (Approx 1.0 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofAspirin (Whole)

21

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Beta Carotene

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6 iWaveMARS 6

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofBeta Carotene

22

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Biotin

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofBiotin

23

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Calcium Carbonate

Weigh 0.5 g of the sample into the digestion vessel. Slowly add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

Allow initial reaction to subside before sealing vessel.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofCalcium Carbonate

24

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Calpan Pantothenic Acid

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofCalpan Pantothenic Acid

25

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Cephalexin

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofCephalexin

26

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Chromium Chelate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofChromium Chelate

27

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Cold and Flu Medicine (Liquid)

Add 0.75 mL of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.75 mL Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofCold and Flu Medicine (Liquid)

28

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Cupric Sulfate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, blue in color, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofCupric Sulfate

29

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Diclofenac K

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

H2O2 (30%)

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofDiclofenac K

30

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Empty Capsule (gel)

Weigh 1 capsule (Approx 0.1 g) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

1 capsule (Approx 0.1 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofEmpty Capsule (gel)

31

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Fish Oil (No Capsule)

Weigh 0.25 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.25 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofFish Oil (No Capsule)

32

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Folic Acid

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofFolic Acid

33

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Ginko (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofGinko (Ground)

34

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Ginko (Whole Pill)

Weigh 1 pill (approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3H2O2

1 pill (Approx 1.0 g) Organic Ramp to Temperature One Touch

210 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofGinko (Whole Pill)

35

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Kelp

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofKelp

36

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Losataran K

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3H2O2 (30%)

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofLosataran K

37

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Magnesium Carbonate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

Allow any reaction to subside before sealing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofMagnesium Carbonate

38

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Magnesium Oxide

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofMagnesium Oxide

39

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Manganese Carbonate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofManganese Carbonate

40

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Metaformin (ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofMetaformin (ground)

41

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Multivitamin (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofMultivitamin (Ground)

42

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Multivitamin (Whole)

Weigh 1 pill (Approx 2.5 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep Plus

HNO3

HCl

1 pill (Approx 2.5 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofMultivitamin (Whole)

43

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Niacinamide

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofNiacinamide

44

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Pantothenic Acid

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofPantothenic Acid

45

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Potassium Chelate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofPotassium Chelate

46

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Pyridoxine

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofPyridoxine

47

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Riboflavin

Weigh 0.25 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep Plus

HNO3HCl

0.25 g Organic Ramp to Temperature One Touch

230 20:00 30:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofRiboflavin

48

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Selenium Chelate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofSelenium Chelate

49

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Stearic Acid

Weigh 0.25 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep Plus

HNO3

0.25 g Organic Ramp to Temperature One Touch

210 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofStearic Acid

50

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Thiamine

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofThiamine

51

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Trixie Phosphate in iPrep

Weigh 0.1 g of the sample into the digestion vessel. Add 4 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes to pre-digest before closing the vessel.

This application can only be run in the iPrep vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6 iWave iPrep HNO3HCl

0.1 - 0.5 g Organic Ramp to Temperature Classic

260 30:00 25:00 N/A 1800 Off

Sample was clear, colorless and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on yoursample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofTrixie Phosphate in iPrep

52

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

USP 232/233 (Pharmaceuticals)

Weigh 0.2 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml HCl. Gently swirl the mixture before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpress PlusEasyPrepEasyPrep Plus

HNO3HCl

0.2 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofUSP 232/233 (Pharmaceuticals)

53

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vitamin B-12

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVitamin B-12

54

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vitamin C (ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVitamin C (ground)

55

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vitamin C (whole)

Weigh 1 pill (Approx 1.4 g) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

1 pill (Approx 1.4 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVitamin C (whole)

56

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vitamin D

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

210 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVitamin D

57

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vitamin D3

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVitamin D3

58

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vitamin E

Weigh 0.25 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep Plus

HNO3HCl

0.25 g Organic Ramp to Temperature One Touch

230 20:00 30:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVitamin E

59

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Vitamins

Weigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 9 ml of HNO3, 1 ml HCl, and 1 ml of HF. Gently swirl themixture and wait approximately 15 minutes before closing the vessel.

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additionalcomplexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl willvary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

MARS 6MARS 6 iWave

MARSXpressMARSXpress PlusEasyPrepEasyPrep Plus

HNO3

HFHCl

0.5 g Organic Ramp to Temperature One Touch

210 15:00 30:00 800 900-1050 Off

Sample was clear, colorless and particle free upon dilution to 50 ml.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results onyour sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofVitamins

60

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Zinc Sulfate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3HCl

0.5 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofZinc Sulfate

Plastics, Polymers, & Oils

MARS™ 6

62

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Mineral Oil

Weigh 0.25 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

HNO3

0.25 g Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofMineral Oil

Discover® SP-D 80

Consumer Products

64

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Suppository

Weigh 1 Capsule (approx. 1 g) of the sample into the digestion vessel. Add 12 mL of HNO3. Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

The use of HF may be required to digest any silicates found in the sample.

Discover SP-D 80 mL QuartzQuartz with Teflon liner

HNO3

1 capsule Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) If using HF follow restictions listed in HF addendum.

Microwave Digestion ofSuppository

65

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Vaseline

Weigh 0.5 g of the sample into the digestion vessel. Add 8 mL of HNO3 and 2 mL of HF. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additionalcomplexation step with boric acid should be used. This procedure can be found in the method note entitled “Boric Acid HF Neutralization”.

Discover SP-D 80 mL Quartz with Teflon liner HNO3HF

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) If using HF follow restictions listed in HF addendum.

Microwave Digestion ofVaseline

66

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Wax (candle)

Weigh 0.5 g of the sample into the digestion vessel. Add 8 mL of HNO3 and 2 mL of HF. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additionalcomplexation step with boric acid should be used. This procedure can be found in the method note entitled “Boric Acid HF Neutralization”.

Discover SP-D 80 mL Quartz with Teflon liner HNO3HF

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) If using HF follow restictions listed in HF addendum.

Microwave Digestion ofWax (candle)

Nutraceuticals

Discover® SP-D 80

68

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Ginko (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15 minutes beforecapping the vessel.

Discover SP-D 80 mL Quartz HNO3

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofGinko (Ground)

69

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Ginko (Whole Pill)

Weigh 1 pill (approx 1 g) of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

1 pill Organic Ramp to Temperature

210 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofGinko (Whole Pill)

70

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Kelp

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofKelp

Pharmaceutical & Biotech

Discover® SP-D 80

72

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Allergy Pill (ground)

Weigh 0.25 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL H2O2 (30%). Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

0.25 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofAllergy Pill (ground)

73

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Allergy Pill (whole pill)

Weigh 1 pill (approx 1 g) into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

1 pill Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofAllergy Pill (whole pill)

74

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Antioxidant (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HClwill vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3 HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofAntioxidant (Ground)

75

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Antioxidant (whole pill)

Weigh 1 pill (approx1.4 g) of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

1 pill Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofAntioxidant (whole pill)

76

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Aspirin (Ground)

Weigh 0.25 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL H2O2 (30%). Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

0.25 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofAspirin (Ground)

77

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Aspirin (Whole)

Weigh 1 pill (approx 1 g) of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

1 pill Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofAspirin (Whole)

78

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Calpan Pantothenic Acid

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofCalpan Pantothenic Acid

79

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Cephalexin

Add1 mL of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15 minutes beforecapping the vessel.

Discover SP-D 80 mL Quartz HNO3

1 mL Organic Ramp to Temperature

210 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofCephalexin

80

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Chromium Chelate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofChromium Chelate

81

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Cold and Flu Medicine (liquid)

Add 0.75 mL of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL H2O2 (30%) Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

0.75 mL Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofCold and Flu Medicine (liquid)

82

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Diclofenac K

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%) Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofDiclofenac K

83

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Empty Capsule

Weigh 0.1 g of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15 minutes beforecapping the vessel.

Discover SP-D 80 mL Quartz HNO3

0.1 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofEmpty Capsule

84

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Fish Oil (No Capsule)

Weigh 0.25 g of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15 minutes beforecapping the vessel.

Discover SP-D 80 mL Quartz HNO3

0.25 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofFish Oil (No Capsule)

85

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Folic Acid

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HClwill vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofFolic Acid

86

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Ginko (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15 minutes beforecapping the vessel.

Discover SP-D 80 mL Quartz HNO3

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofGinko (Ground)

87

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Ginko (Whole Pill)

Weigh 1 pill (approx 1 g) of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

1 pill Organic Ramp to Temperature

210 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofGinko (Whole Pill)

88

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Kelp

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofKelp

89

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Losataran K

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofLosataran K

90

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Magnesium Oxide

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofMagnesium Oxide

91

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Manganese Carbonate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofManganese Carbonate

92

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Metaformin

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofMetaformin

93

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Multivitamin (ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and wait approximately15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofMultivitamin (ground)

94

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Multivitamin (whole)

Weigh 1 pill (approx 2.5 g) of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and waitapproximately 30 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

1 pill Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofMultivitamin (whole)

95

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Stearic Acid

Weigh 0.25 g of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15 minutes beforecapping the vessel.

Discover SP-D 80 mL Quartz HNO3

0.25 g Organic Ramp to Temperature

210 10:00 10:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofStearic Acid

96

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Thiamine

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofThiamine

97

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

USP 232/233

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl . Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofUSP 232/233

98

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Vitamin C (ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO3. Gently swirl the mixture and wait approximately 15 minutes beforecapping the vessel.

Discover SP-D 80 mL Quartz HNO3

0.5 g Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofVitamin C (ground)

99

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Vitamin C (whole)

Weigh 1 pill (approx1.4 g) of the sample into the digestion vessel. Add 10 mL HNO3 and 2 mL H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before capping the vessel.

Discover SP-D 80 mL Quartz HNO3

H2O2 (30%)

1 pill Organic Ramp to Temperature

200 5:00 3:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofVitamin C (whole)

100

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Vitamin D3

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofVitamin D3

101

Procedure

Notes

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

1

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type

Reagents

Max Sample Weight Sample Type / Vent Program

www.cem.com Form prepared by:

Discover® SP-D 80TM

Method Note

Results

General Precautions

Zinc Sulfate

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before capping the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl \will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties ofanalysis.

Discover SP-D 80 mL Quartz HNO3

HCl

0.5 g Organic Ramp to Temperature

200 5:00 5:00 400 300 Med

Sample was clear, colorless, and particle free upon dilution to 50 mL.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.

Microwave Digestion ofZinc Sulfate

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