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Energy Transformation: Cellular Respiration Outline
1. Energy and carbon sources in living cells 2. Sources of cellular ATP 3. Turning chemical energy of covalent bonds between C-C into
energy for cellular work (ATP) 4. Importance of electrons and H atoms in oxidation-reduction
reactions in biological systems 5. Cellular mechanisms of ATP generation 6. The four major central metabolic pathways
Glycolysis- Fermentation Transition step Krebs Cycle Electron Transport chain and oxidative phosphorylation
4. Poisons of cellular respiration and their mechanisms of action. 5. Use of biomolecules other than glucose as carbon skeletons &
energy sources
http://health.howstuffworks.com/sports-physiology3.htm
A cell has enough ATP to last for about three seconds
Necessary ATP is supplied to muscle cells by
1. Phosphagen system (8 to 10 seconds.) 2. Glycogen-lactic acid system (90 seconds)- glucose 3. Aerobic Respiration (unlimited)- glucose
Phosphagen system (muscle fibers)
(*) Arezki Azzi et al. Protein Sci 2004; 13: 575-585
• Creatine phosphate contains (a high-energy phosphate compound).
• Creatine kinase transfers the phosphate to ADP to form ATP.
Creatine-Phosphate (tri)
*
Creatine-Phosphate + ADP (Tri)
Creatine-Phosphate + ATP (Di)
Creatine Kinase
During cellular respiration the potential energy in the chemical bonds holding C atoms in organic molecules in turned into ATP.
In presence of oxygen complete breakdown
C-C-C-C-C-C + O2……………… CO2 + H2O + ATP large amount
In absence of oxygen
incomplete breakdown C-C-C-C-C-C ……………… 2 C-C-C + ATP
small amount
Cellular respiration: ATP is produced aerobically, in the presence of oxygen
Fermentation: ATP is produced anaerobically, in the absence of oxygen
Chemical bonds of organic molecules
Chemical bonds involve electrons of atoms Electrons have energy Chemical bonds are forms of potential energy If a molecule loses an electron does it lose energy? When a chemical bond is broken sometimes electrons can be released
• Oxidation: removal of electrons. • Reduction: gain of electrons. • Redox reaction: oxidation reaction paired with a
reduction reaction.
Oxidation-Reduction
The electrons associated with hydrogen atoms. Biological oxidations are often dehydrogenations
Soluble electron carriers NAD+, and FAD (ATP generation) NADP+ (biosynthetic reactions)
Electron Carriers in Biological Systems
Nicotinamide Adenine Dinucleotide (NAD+) a co-enzyme that acts as an electron shuttle (Niacin)
NAD+
Nicotinamide (oxidized form)
Dehydrogenase
2 e– + 2 H+
2 e– + H+
NADH H+
H+
Nicotinamide (reduced form)
+ 2[H] (from food)
+
Flavin Adenine Dinucleotide (FAD+) is another co-enzyme that acts as an electron shuttle (Vitamin B2)
In cells, energy is harvested from an energy source (organic molecule) by a series of coupled oxidation/reduction reactions (redox) known as the central metabolic pathways
becomes oxidized
becomes reduced
Dehydrogenase
An overview of the central metabolic pathways • Glycolysis • Transition step • Tricarboxylic acid cycle (TCA or Krebs cycle) • The Electron Transport Chain and oxidative
phosphorylation
2. Oxidative phosphorylation/ Chemiosmosis
Mechanisms of ATP Generation during cellular respiration
• ADP and inorganic PO-4
• ATP synthase • Cellular respiration in mitochondria • Electron Transport Chain
Glycolysis
• Multi – step breakdown of glucose into intermediates
• Turns one glucose (6-carbon) into two pyruvate (3-carbon) molecules
• Generates a small amount of ATP (substrate- level phosphorylation)
• Generates reducing power - NADH + H+
1. Substrate-level phosphorylation
Generation of ATP
-Transfer of a high-energy PO4 from an organic molecule to ADP - Different enzymes - During glycolysis and the TCA or Krebs cycle
-
Fermentation
• An extension of glycolysis • Takes place in the absence of oxygen (anaerobic) • Regenerates NAD+ from NADH + H +
• ATP generated by substrate - level phosphorylation during glycolysis
• Does not use the Krebs cycle or ETC (no oxidative - phosphorylation)
• A diversity of end products produced (i.e. lactic acid, alcohols, etc.)
Energy investment phase
Glucose
2 ATP used 2 ADP + 2 P
4 ADP + 4 P 4 ATP formed
2 NAD+ + 4 e– + 4 H+
Energy payoff phase
2 NADH + 2 H+
2 Pyruvate + 2 H2O
Net
2 Pyruvate + 2 H2O
2 ATP
2 NADH + 2 H+
Glucose
4 ATP formed – 2 ATP used
2 NAD+ + 4 e– + 4 H+
Glycolysis Citric acid cycle
Oxidative phosphorylation
ATP ATP ATP
The energy input and output of glycolysis
Transition step
• Under aerobic conditions, the pyruvate produced during glycolysis is directed to the mitochondrion.
• A multi-enzyme complex, spanning the 2 mitochondrial membranes, turns pyruvate (3-carbon) into Acetyl-CoA (2-carbon) and releases one CO2 molecule
• Uses coenzyme A (Vit B derivative) • Generates reducing power NADH + H+
Figure 9.10 Conversion of pyruvate to acetyl CoA, the junction between glycolysis and the Krebs cycle
Krebs or TCA Cycle
• A cyclical pathway • Accepts – acetyl – CoA • Generates 2 – CO2 molecules (for every acetyl-CoA • Generates reducing power NADH + H+ and FADH2
• Generates a small amount of ATP (substrate-level phosphorylation)
Electron Transport Chain and ATP generation
Electron transport chain: • membrane-embedded or
bound electron carriers • Mostly proteins with non-
protein groups. • NADH vs. FADH2
• No direct formation of ATP • Pumps H+ into the inter-
membrane space
2. Oxidative phosphorylation/ Chemiosmosis
ATP Generation during aerobic cellular respiration
• ADP and inorganic PO-4
• ATP synthase • Cellular respiration in mitochondria • Electron Transport Chain
Oxidative Phosphorylation and ATP generation
ATP Generation : • Membrane-embedded protein complex , ATP
synthase • Proton-motive force • Direct formation of ATP by Chemiosmosis Chemiosmosis: the coupling of the transport of
H+ across membrane by facilitated diffusion with chemical reaction producing ATP
Electron Transport chains and oxidative phosphorylation http://highered.mcgraw-hill.com/sites/0072437316/student_view0/chapter9/animations.html# ATP Synthase Gradient: The Movie http://vcell.ndsu.edu/animations/at pgradient/movie.htm
Certain poisons interrupt critical events in cellular respiration • Block the movement of electrons • Block the flow of H+ through ATP synthase • Allow H+ to leak through the membrane
H+
H+
H+
H+
H+
H+ H+ H+ H+
+ 2 H+
H+
H+ H+
1 O2 2
H O 2 ADP + P ATP
NADH FADH2
NAD+
FAD
Rotenone Cyanide, carbon monoxide
Oligomycin
DNP
ATP Synthase
Electron Transport Chain Chemiosmosis
Energy yield: How many ATP molecules are generated during cellular respiration of a single glucose molecule?
Metabolic Disorders
http://www.merckmanuals.com/professional/pediatrics/inherited-disorders-of-metabolism/overview-of-carbohydrate-metabolism-disorders
Find out the Specific defect Symptoms
1. Pyruvate dehydrogenase deficiency (Transition Step) 2. Mitochondrial Oxidative Phosphorylation Disorders
Sources of cellular glucose
• glucose from food in the intestine • glycogen supplies in the muscles • breakdown of the Liver's glycogen into
glucose
The Catabolism of various food molecules
Beta oxidation
http://www.brookscole. com/chemistry_d/templ ates/student_resources /shared_resources/ani mations/carnitine/carni tine1.html
• Triglycerides consist of glycerol and fatty acids • Fatty acids broken down into through beta
oxidation
Simple lipids
Proteins
Protein Amino acids Extracellular proteases
Krebs cycle and glycolysis Transition step
Deamination, decarboxylation, dehydrogenation Organic acid
Feedback control of cellular respiration
Phosphofructokinase
Allosteric enzyme
Activity modulated by inhibitors and activators
Adjustment of cellular respiration in response to demand
OXIDATIVE PHOSPHORYLATION (Electron Transport and Chemiosmosis)
Food, such as peanuts
Carbohydrates Fats Proteins
Sugars Glycerol Fatty acids Amino acids
Amino groups
Glucose G3P Pyruvate Acetyl CoA
CITRIC ACID CYCLE
ATP
GLYCOLYSIS