CellEase® II SeriesFor fast and simple DNA extraction

Blood

Plant

MeatTissue

Bacteria

BloBBBlBlolo

Features and Advantages

　● Rapid and efficient DNA extraction　● Easy and simple protocol to prepare PCR grade template DNA　● Low cost　● Use for research and laboratory food industry

① 100 μl of CellEase® mixtureReagent A 20 μlReagent B 20 μlDistilled water 60 μl

② 2~10 mg sample

Mix CellEase® mixture and sample

Incubate at specified temperatures72℃, 6 min94℃, 3 min

Use 1~10 μl of extracted DNA as a template for PCR(Total 50 μl reaction volume)

Applicable Samples:Tissue, Bacteria, Meat, Blood and plants.

R grade template DNA

ustry

CellEase Mix

Distilled water

PCR

Phenolreagent

Centrifugalseparation

Ethanolprecipitation

Alkalinereagent

ConventionalCellEase

Generalmethod

Sample

CellEase® II Mix

Centrifugalseparation

Only 1 step +

CellEase® II Series For fast and simple DNA extraction

DNA Extraction Examples

Ordering Information

DNA Extraction Examples

DNA extraction from mouse tail

M:Marker (100 bp ladder)1: DNA extract by using CellEase® TissueⅡ conc.2: DNA extract by using CellEase® TissueⅡ conc.×103: DNA extract by using CellEase® TissueⅡ conc.×1004: DNA extract by using conventional CellEase® conc.5: DNA extract by using conventional CellEase® conc.×106: DNA extract by using conventional CellEase® conc.×100

DNA extraction from tomato leaf

M: Marker（500 bp ladder）1: + CellEase®, Without dilution Add 5 μl to PCR2: + CellEase®, ×10 dilution Add 5 μl to PCR3: + CellEase®, × 100 dilution Add 5 μl to PCR4: ‐ CellEase®, Without dilution Add 5 μl to PCR5: ‐ CellEase®, ×10 dilution Add 5 μl to PCR6: ‐ CellEase®, × 100 dilution Add 5 μl to PCR7: + CellEase®, Without dilution Add 6 μl to PCR8: + CellEase®, ×10 dilution Add 6 μl to PCR9: + CellEase®, × 100dilution Add 6 μl to PCR

10: ‐ CellEase®, Without dilution Add 6 μl to PCR11: ‐ CellEase®,×10 dilution Add 6 μl to PCR12: ‐ CellEase®,× 100 dilution Add 6 μl to PCR13: + CellEase®, Without dilution Add 7 μl to PCR14: + CellEase®, ×10 dilution Add 7 μl to PCR15: + CellEase®, × 100 dilution Add 7 μl to PCR16: ‐ CellEase®, Without dilution Add 7 μl to PCR17: ‐ CellEase®, ×10 dilution Add 7 μl to PCR18: ‐ CellEase®, × 100 dilution Add 7 μl to PCR

DNA extraction from Staphylococcus. aureus

M Marker (500 bp ladder)1: Undiluted Sample Add 5 μl of DNA extract to PCR2: x 10 dilution3: x 102 dilution4: x 103 dilution5: x 104 dilution6: Undiluted Sample Add 6 μl of DNA extract to PCR7: x 10 dilution8: x 102 dilution9: x 103 dilution10: x 104 dilution

DNA extraction from whole blood

Sample: Whole blood (EDTA)M: Marker (100 bp ladder)1: CellEase® Blood2: CellEase® Blood 3: CellEase® Blood 4: Extraction by water

DNA extraction from fish (Tuna) samples

M: Marker（100 bp ladder）1: Sample 6 mg, Add 5 μl of DNA extract to PCR2: Sample 6 mg, Add 6 μl of DNA extract to PCR3: Sample 6 mg, Add 7 μl of DNA extract to PCR4: Sample 9 mg, Add 5 μl of DNA extract to PCR5: Sample 9 mg, Add 6 μl of DNA extract to PCR6: Sample 9 mg, Add 7 μl of DNA extract to PCR7: Sample 18 mg, Add 5 μl of DNA extract to PCR8: Sample 18 mg Add 6 μl of DNA extract to PCR9: Sample 18 mg Add 7 μl of DNA extract to PCR

Product Name Catalog Number Size Storage

CellEase® Tissue II BIC-BCR10-00001

CellEase® Bacteria II BIC-BCR10-00002

CellEase® Meat II BIC-BCR10-00003 50 Extractions 4℃

CellEase® Blood BIC-BCR11-00001

CellEase® Plant BIC-BCR11-00002

10080For research use only, Not for diagnostic use.　　　 www.cosmobio.com

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