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CECT 434 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Other culture collections: ATCC 25922; CCM 3954; CCRC 14902; CCTM La 2184; CCUG 7736; CCUG 17620; CCUG 21456; CIP 76.24; CNCTC Ec 327/73; DSM 1103; FDA Seattle 1946; GISK 240533; HER 1176; IFO 15034; JCM 5491; LMG 8223; NCIMB 12210; PCM 2057; WDCM 00013 History: CECT, 1978 < ATCC < FDA < F. Schoenknecht Isolation source: Clinical isolate Applications: Reference Strain recommended to be used for UNE-CEN ISO/TS 11133, EN ISO 6888 and ISO 15214 Risk group: 2 Culture conditions (medium, incubation temperature, incubation time): 1, 37ºC, 24h CHARACTERIZATION Miniaturized biochemical test API 20E Growth on selective/differential culture media CECT Certified Strains have been selected as typical representatives of species commonly used as reference material after testing that their biochemical profile and their colony appearance on selective/ differential media conform to the expected results. ENDO AGAR Red colonies, red medium, golden sheen TERGITOL AGAR Yellow colonies with yellow zone. Sometimes with rust coloured centre + - + + - - - - + - - + + - + + - + - + - 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 ONPG ADH LDC ODC CIT H 2 S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX 5 1 4 4 5 5 2 Identification: 99,9% ID Escherichia coli C E R T I F I E D CERTIFICADA 20121024 TBX (Tryptone Bile X- glucuronide) AGAR Blue-green colonies Page 1 of 2

CECT 434 Escherichia coli · 2013. 11. 5. · CECT 434 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Growth on selective/differential culture media C E R T I F I E D

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Page 1: CECT 434 Escherichia coli · 2013. 11. 5. · CECT 434 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Growth on selective/differential culture media C E R T I F I E D

CECT 434 Escherichia coli(Migula 1895) Castellani and Chalmers 1919

Other culture collections: ATCC 25922; CCM 3954; CCRC 14902; CCTM La 2184; CCUG 7736; CCUG 17620; CCUG 21456; CIP 76.24; CNCTC Ec 327/73; DSM 1103; FDA Seattle 1946; GISK 240533; HER 1176; IFO 15034; JCM 5491; LMG 8223; NCIMB 12210; PCM 2057; WDCM 00013

History: CECT, 1978 < ATCC < FDA < F. SchoenknechtIsolation source: Clinical isolate

Applications: Reference Strain recommended to be used for UNE-CEN ISO/TS 11133, EN ISO 6888 and ISO 15214

Risk group: 2Culture conditions (medium, incubation temperature, incubation time): 1, 37ºC, 24h

CHARACTERIZATION

Miniaturized biochemical test API 20E

Growth on selective/differential culture media

CECT Certified Strains have been selected as typical representatives of species commonly used as reference material after testing that their biochemical profile and their colony appearance on selective/ differential media conform to the expected results.

ENDO AGAR

Red colonies, red medium, golden sheen

TERGITOL AGAR

Yellow colonies with yellow zone. Sometimes with rust coloured centre

+ - + + - - - - + - - + + - + + - + - + -

1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4

ONPG ADH LDC ODC CIT H2S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX

5 1 4 4 5 5 2

Identification: 99,9% ID Escherichia coli

C E R T I F I E D

C E R T I F I C A D A

20121024

TBX (Tryptone Bile X-glucuronide) AGARBlue-green colonies

Page 1 of 2

Page 2: CECT 434 Escherichia coli · 2013. 11. 5. · CECT 434 Escherichia coli (Migula 1895) Castellani and Chalmers 1919 Growth on selective/differential culture media C E R T I F I E D

CECT 434 Escherichia coli(Migula 1895) Castellani and Chalmers 1919

Growth on selective/differential culture media

C E R T I F I E D

C E R T I F I C A D A

CTSM (Sorbitol MacConkeyagar with Cefixime Tellurite) AGARPink colonies

VRBL (Violet Red Bile Lactose Agar) AGAR

Red-purple colonies with or without halos

VRBGA (Violet Red Bile Glucose Agar) AGAR

Red-purple colonies surrounded by purple haloes

20121024Page 2 of 2

MYP (Mannitol Egg YolkPolymyxin) AGARGrowth inhibition

Left: Brilliant Green Bile Lactose BrothRight: Laurylsulfate Tryptose BrothBoth: Turbidity with gas production

EC Broth

Turbidity with gas production