CATÁLOGO MICROGEN MICROBIOLOGÍA 2013, AKRALAB

Pulsar Aquí

1.- BACILLUS- ID

2.- C. DIFFICILE

3.- CAMPYLOBACTER

CATÁLOGO MICROGEN MICROBIOLOGÍA 2013, AKRALAB

4.- E. COLI 0157

5.- LEGIONELLA RAPID

6.- LISTERIA

7.- SALMONELLA

8.- STAPH

9.- STREP

10.- GN A + B - ID

11.- LISTERIA ID

12.- STAPH ID

13.- STREP ID

14.- PATH-CHEK HYGIENE PATHOGEN SYSTEM

15.- PATH-CHEK HYGIENE PROTEIN SWAB

16.- ROTAVIRUS SCREENING

17.- ADENOVIRUSS SCREENING

18.- HELICOSCREEN

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Protecting Food and Health

MICROGEN BIOPRODUCTS LTD

MICROGEN® BACILLUS-IDAN IDENTIFICATION SYSTEM FOR THE MESOPHILIC BACILLUS

SPECIES AND RELATED GENERA ASSOCIATED WITH FOOD POISONING AND FOOD SPOILAGE

PRODUCT CODE: MID-66 (20 TEST KIT)

♦ Substrates used are based on conventional methods and fully validated inscientific literature

♦ Simple and easy-to-use 24 reaction system♦ Results in 48 hours♦ Results interpreted using new Microgen ID system software (registered

users are entitled to free database updates)♦ System designed specifically for Bacillus

Phone: +44 1276 600081 Fax: +44 1276 600151 E-mail: [email protected] www.microgenbioproducts.com

1 Admiralty Way Camberley Surrey GU15 3DT United Kingdom


Improved substrates: stronger, clearer

reactions

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Mesophilic Bacillus spp. identified:

Bacillus species B. cereus group (includes B. cereus, B. thuringiensis B. mycoides and B. weihenstephanensis)

B. firmus B. megaterium B. pumilus B. licheniformis B. badius B. laevolacticus Virgibacillus species B. coagulans V. pantothenticus B. lentus B. amyloliquefaciens Paenibacillus species B. subtilis P. alvei B. circulans P. polymyxa B. insolitus P. macerans B. thiaminolyticus B. freudenreichii Brevibacillus species B. globisporus Br. brevis B. sphaericus Br. laterosporus

Additional reagents available: • MID61A Nitrate A reagent • MID61B Nitrate B reagent • MID61C VP I reagent • MID61D VP II reagent • MID61E TDA reagent • MID61F Indole Kovacs reagent • MID61G Oxidase Strips • MID61H Mineral oil • MID61K PYR reagent

Other Identification Systems available: • MID-64 Microgen GN-ID A • MID-65 Microgen GN-ID B • MID-67 Microgen Listeria-ID • MID-69 Microgen Staph-ID

As with all Microgen Bioproducts biochemical ID systems Microgen Bacillus-ID is supported by the simplest-to-use and most feature-packed Computer Identification System Software (MID60) available, delivering the most comprehen-sive and up-to-date databases which registered users can update free-of-charge from www.microgenbioproducts.com

MicrogenTM Bacillus-ID has been developed for the identification of mesophilic Bacillus spp. and related genera isolated from food, food ingredients and beverages either as food spoilage or food poisoning organisms. B. cereus and to a lesser extentB. licheniformis are well known as causative agents of food poisoning. Numerous other species and related genera including B. pumilus, B. thuringiensis, B. brevis,B. mycoides, Paenibacillus alvei, B. circulans and B. sphaericus have also been reported as causing food poisoning.

The identification of these species has been difficult due to a combination of factors including:

• Confusion over the taxonomy ofBacillus spp.

• Variable performance in conventionalbiochemical test media

• Extensive range of conventionalbiochemical media required

• Reliance on microscopic endosporemorphology

Microgen™ Bacillus-ID overcomes all of these problems with a simple and convenient identification system presented in a convenient microwell format.

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MICROGEN® C. DIFFICILE RAPID TEST

A UNIQUE RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF CLOSTRIDIUM DIFFICILE CULTURED ON SELECTIVE SOLID MEDIA FROM FAECAL SAMPLES.

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE MICROGEN BIOPRODUCTS C. DIFFICILE CULTURE CONFIRMATION ASSAY OFFER:

RESULTS IN TWO MINUTES INCREASED EFFICIENCY HIGH SENSITIVITY

HIGH SPECIFICITY ACCURATE & RELIABLE RESULTS

Phone: +44 (0)1276 600081 Fax:: +44 (0)1276 600151 E-mail: [email protected] www.microgenbioproducts.com


M ICROGEN B IOPRODUCTS LTD

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Microgen C. difficile rapid test is part of a range of rapid tests which includes;

Microgen Salmonella (M42CE) Microgen Staph (M43CE & M433CE) Microgen E. coli 0157 (M44CE) Microgen Legionella (M45CE) Microgen Campylobacter (M46CE) Microgen Strep (M47CE)

Code M41CE Description Microgen™ C. difficile Size 50 tests Storage 2 - 8°C

Clostridium difficile has a well documented role in pseudomembraneous colitis and diarrhoea associated with antibiotics. Evidence has also implicated C. difficile in colitis not induced by antibiotics, exacerbations of chronic inflammatory bowel disease and postoperative diarrhoea. Many laboratories rely on the detection of C. difficile cytotoxin to diagnose infection. However, investigations have shown that the detection of faecal cytotoxin is not always consistent with active infection. This, plus the fact that some laboratories do not have the specialised facilities that are required to detect faecal cytotoxin, has highlighted the need for a test that is easy to use and gives rapid results for the identification of C. difficile.

Use of Microgen® C. difficile allows identification of C. difficile from selective agar, within two minutes.

Traditional methods for identification of C. difficile includes GLC and cytotoxin detection. However, GLC is expensive in terms of time, equipment and cost per test and the link between presence of cytotoxin and symptoms is inconsistent. The test latex particles in Microgen® C. difficile are coated with antibodies specific for C. difficile somatic antigens, resulting in a highly specific test with a high predictive negative value. The flexibility of the test means that it can be used to test isolates directly form selective agar plates, such as CCFA.

The kit, which is simple-to-use and gives clear cut results, contains a positive control, has a long shelf-life, and offers the confidence of high sensitivity and specificity.

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MICROGEN® CAMPYLOBACTER RAPID TEST

A RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF ENTEROPATHOGENIC, THERMOPHILIC CAMPYLOBACTER GROWN ON SELECTIVE MEDIA

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE MICROGEN BIOPRODUCTS CAMPYLOBACTER CULTURE CONFIRMATION ASSAY OFFER:


HIGH SPECIFICITY ACCURATE & RELIABLE RESULTS




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2006-09

Microgen Campylobacter Rapid Test detects:

• C. jejuni• C. jejuni subsp. Doylei• C. coli• C. upsaliensis• C. laridis• C. fetusMicrogen Campylobacter rapid test is part of a range of rapid tests which includes;

Microgen C. difficile (M41CE) Microgen Salmonella (M42CE) Microgen Staph (M43CE & M433CE) Microgen E. coli 0157 (M44CE) Microgen Legionella (M45CE) Microgen Campylobacter (M46CE) Microgen Strep (M47CE)

All rapid tests use latex agglutination technology. Code M46CE Description Microgen®

Campylobacter Size 50 tests Storage 2 - 8°C

Campylobacter gastro-enteritis is most prevalent in young children, but outbreaks are also common in adults. In temperate climates the incidence of Campylobacter gastro-enteritis peaks in summer and early autumn, whereas in tropical climates peak incidences may occur in the cooler months. Campylobacter infection may be effectively treated with antibiotics but in the majority of cases, due to the length of time required by traditional detection methods, the acute phase has passed by the time a laboratory diagnosis is communicated to the physician. Microgen® Campylobacter is a rapid, easy-to-use test which can be reliably performed on selective agar colonies. Microgen® Campylobacter can therefore reduce the time taken to produce a laboratory diagnosis and improve patient prognosis.

Microgen Campylobacter... • Detects Campylobacter from solid selective media.

• Permits the early detection of Campylobacter gastro-enteritis enablingappropriate therapy to commence immediately, thereby improving patientprognosis.

• Is highly specific so that a presumptive identification can be reliably formed onthe basis of the Microgen® test alone.

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MICROGEN® E. COLI 0157 RAPID TEST

A RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF E. COLI SEROGROUP 0157 CULTURED ON SELECTIVE SOLID MEDIA

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE

MICROGEN BIOPRODUCTS E. COLI 0157 CULTURE CONFIRMATION ASSAY OFFER: RESULTS IN TWO MINUTES INCREASED EFFICIENCY HIGH SENSITIVITY HIGH SPECIFICITY ACCURATE & RELIABLE RESULTS




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Code M44CE Description Microgen® E. coli 0157 Size 50 tests Storage 2 - 8°C

Much of the rapidly expanding interest in E. coli 0157 results from the increasing capability to isolate and identify this organism. E. coli 0157 is a verotoxin (VT) producing strain responsible for Haemorrhagic colitis (HC), Haemolytic Uraemic Syndrome (HUS) and community acquired diarrhoea. Until recently it was thought that E. coli 0157 was only isolated from samples of bloody diarrhoea as this is classically seen with verotoxin producing strains. However, it is now increasingly being isolated from non-bloody specimens. Sorbitol MacConkey medium is the recommended screening agar for E. coli 0157, but, although this technique shows 100% sensitivity it is only 85% specific. E. coli 0157 does not ferment sorbitol. However, other sorbitol negative Gram negative rods also grow on sorbitol MacConkey agar. As a result of this, a specific rapid identification method is required. Microgen® E. coli 0157 is an easy-to-use one step, latex identification kit which gives results in under two minutes and specifically identifies E. coli 0157 directly from the selective plate, even in mixed culture.

The conventional method for identification of E. coli 0157:H7 utilises specific antisera in a standard agglutination test. However, this method and other commercially available latex tests have been shown to suffer from cross reactions with other enteric organisms such as E. hermanii, which antigenically and biochemically resembles E. coli 0157, resulting in false positive results. Microgen® E. coli 0157 contains a test latex which has been sensitised with E. coli 0157 specific antibodies, that are prepared by absorption with potentially cross reacting strains. Microgen® E. coli 0157 has demonstrated no cross reactions when tested against a panel of 161 E. coli 0157 ‘O’ serotypes and 56 ‘H’ serotypes, and shows strong specific agglutination. Microgen® E. coli 0157 gives strong and specific results within two minutes and comes complete with a control latex which can be easily compared with test results to confirm non-specific reactions.

Microgen E. coli 0157 rapid test is part of a range of rapid tests which includes; Microgen C. difficile (M41CE) Microgen Salmonella (M42CE) Microgen Staph (M43CE & M433CE) Microgen E. coli 0157 (M44CE) Microgen Legionella (M45CE) Microgen Campylobacter (M46CE) Microgen Strep (M47CE) All rapid tests use latex agglutination technology.

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MICROGEN® LEGIONELLA RAPID

A RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF LEGIONELLA PNEUMOPHILA SEROGROUP 1AND SEROGROUP 2-15 AND NON-L. PNEUMOPHILA SPECIES OF CLINICAL

SIGNIFICANCE GROWN ON SELECTIVE MEDIA

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE MICROGEN BIOPRODUCTS LEGIONELLA CULTURE CONFIRMATION ASSAY OFFER:


HIGH SPECIFICITY(NO NEED FOR A CONTROL LATEX) ACCURATE & RELIABLE RESULTS EXCELLENT TECHNICAL SERVICE DETECTION OF L. FEELEI DETECTS SEROGROUP 15 RELIABLE SUPPLY

METHOD FOR MUCCOID SAMPLES FLEXIBLE TESTING




NOW INCLUDES LEGIONELLA

SPECIES REAGENT

SEPARATE REAGENTS NOW

AVAILABLE

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Code M45CE Description Microgen® Legionella Size 50 tests Storage 2 - 8°C

L. pneumophila is the most common cause of Legionnaires Disease. At present 16 serogroups exist, of which L.. pneumophila serogroup 1 accounts for the majority of cases. In addition, a range of other species of Legionella have also been implicated as the causative agent of Legionnaires disease. Clinical and environmental awareness, and testing for Legionella, is rapidly increasing assisted by the introduction of several commercially available selective culture media. However, none of the media are specific for L.. pneumophila serogroup 1 and not everything that grows on them is Legionella. Therefore a specific test is needed to quickly confirm the identification of isolates so that appropriate action can be taken. Microgen® Legionella is an easy-to-use latex agglutination test for L. pneumophila serogroups 1 and 2-15 and now a range of non-L. pneumophila species which have been implicated as causes of diseases in humans. The kit provides specific results in under two minutes directly from a selective medium.

Conventional methods for identification of Legionella utilise specific antisera in standard agglutination or immunofluorescence tests. However, these methods lack sensitivity, are time consuming and can require specialised training and expensive equipment. The test latexes used in Microgen® Legionella are coated with immunoglobulins which have been specially processed to minimise cross reactions with potentially cross reacting organisms, eliminating the need for a control latex, simplifying the procedure and increasing efficiency. Microgen® Legionella has recently been improved by the addition of a new latex reagent which identifies a range of non-L. pneumophila species of clinical significance with a high specificity and no cross-reactions with L. pneumophila. Microgen® Legionella contains test latexes for both L. pneumophila serogroup 1 and 2-15, and now a latex reagent for the following additional species; L. micdadei, L. bozemanii 1 & II, L. dumoffii, L. longbeachae 1 & II, L. jordanis, L. gormanii, L. anisa and L. feelei in a convenient 50 test pack with a long shelf life to offer the most rapid, specific and sensitive test for Legionella currently available.

For convenience the Mirogen® Legionella Rapid Test can be supplied as individual reagents:

Reagent Product Code Detects Quantity

Reagent 1 M45ACE L. pneumophila

serogroup 1

2.5mL

Reagent 2 M45BCE L. pneumophila

serogroup 2-15

2.5mL

Positive Control M45CCE Positive control,

reactive with test

reagents 1, 2-15 and

species.

1.0mL

Reagent Species M45DCE 10 commonly isolated

clinically significant

species

2.5mL

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MICROGEN® LISTERIA RAPID TEST

A UNIQUE RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF LISTERIA spp. FROM COLONIES ON AGAR PLATE MEDIA .

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE MICROGEN BIOPRODUCTS LISTERIA CULTURE CONFIRMATION ASSAY OFFER: RESULTS IN TWO MINUTES INCREASED EFFICIENCY HIGH SENSITIVITY HIGH SPECIFICITY ACCURATE & RELIABLE RESULTS

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Code F48 Description Microgen® Listeria Size 50 Test Storage 2 - 8°C

Listeria spp. are commonly found in soil and are transferred onto vegetable matter. Foods either derived from vegetables or which are derived from grazing animals and which are exposed to minimal processing i.e. dairy products, are the most common vector for the transmission of Listeria spp. to humans.

A total of 6 species have been recognised to date including Listeria monocytogenes which has been associated with human disease. L. monocytogenes is an opportunistic pathogen in that it will only cause disease in people with certain predisposing factors such as the immunosuppressed and pregnant women.

Once colonies have been isolated on agar plates, the next stage requires that any colonies resembling Listeria spp. are tested to confirm that they belong to that genus. Other organisms may be isolated from food samples which may resemble Listeria spp. To confirm the presence of Listeria spp. a number of tests are normally used including aesculin hydrolysis, oxidase, catalase, motility (differential at 25°C and 37°C).

Microgen® Listeria latex was developed as an alternative to this battery of tests. Although individually these tests are inexpensive, they are time consuming to perform. The use of a simple latex agglutination test provides a real-time 2 minute test for the confirmation to genus level of Listeria spp.

Conventional culture methods for Listeria spp. rely on a combination of enrichment and selective media to achieve satisfactory growth of the organism These conventional methods are time consuming and result in unnecessary delays in releasing product from quarantine. Microgen® Listeria can be used to obtain rapid, presumptive-positive results from selective media thus giving an early indication of a failure in the production process. Where standard methods require it, Microgen® Listeria can be used to screen colonies before full biochemical identification and can replace traditional agglutinating antibody methods.

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MICROGEN® SALMONELLA RAPID TEST

A RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF PRESUMPTIVE SALMONELLA COLONIES FROM SELECTIVE

AGAR PLATES

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE MICRO-GEN BIOPRODUCTS SALMONELLA CULTURE CONFIRMATION ASSAY OFFER: RESULTS IN TWO MINUTES INCREASED EFFICIENCY HIGH SENSITIVITY HIGH SPECIFICITY ACCURATE & RELIABLE RESULTS




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Microgen Salmonella rapid test is part of a range of rapid tests which includes; Microgen C. difficile (M41CE) Microgen Salmonella (M42CE) Microgen Staph (M43CE & M433CE) Microgen E. coli 0157 (M44CE) Microgen Legionella (M45CE) Microgen Campylobacter (M46CE) Microgen Strep (M47CE) All rapid tests use latex agglutination technology.

Microgen Salmonella is a latex slide agglutination test for the confirmatory identification of presumptive Salmonella colonies from selective agar plates. The genus Salmonella consists of over 2,000 serotypes which can cause acute infectious gastroenteritis frequently associated with food poisoning. Since many Salmonella spp. infect domestic animals, either clinically or sub-clinically, cases of Salmonella food poisoning usually originate from animal sources. The rapid identification of Salmonella is not only vital for appropriate patient management but also for food manufacturers where prompt results minimise the delay associated with routine testing for Salmonella contamination. The detection and identification of Salmonella spp., using traditional techniques, usually requires identification by serological and biochemical techniques. Microgen® Salmonella offers a rapid alternative to the traditional identification methods and detects Salmonella spp. including S. typhimurium and S. enteritidis.

Code M42CE Description Microgen® Salmonella Size 50 tests Storage 2 - 8°C

Microgen Salmonella…. • Has a very high specificity and a high positive predictive value making it ideal for

testing food, clinical, environmental and veterinary samples. • Detects motile and non-motile strains. • Produces test results in 2 minutes. • Obtained 100% sensitivity in a food sample trial that compared Microgen®

Salmonella with conventional identification techniques thus confirming highly reliable detection of Salmonella.

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MICROGEN® STAPH RAPID TEST

A RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF PRESUMPTIVE STAPHYLOCOCCUS AUREUS COLONIES FROM PRIMARY PLATE CULTURE

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE MICROGEN BIOPRODUCTS STAPH CULTURE CONFIRMATION ASSAY OFFER: RESULTS IN TWO MINUTES INCREASED EFFICIENCY HIGH SENSITIVITY HIGH SPECIFICITY ACCURATE & RELIABLE RESULTS

LATEX CONTROL REAGENT AVAILABLE SEPARATELY




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Code M43CE & M433CE Description Microgen® Staph Size 100 tests/500 test Storage 2 - 8°C

Staphylococcus spp. occur as commensal organisms on the mucous membranes and skin of humans. However, Staphylococcus aureus can be responsible for a wide range of pyogenic infections including superficial suppurant lesions, food poisoning and toxic shock, as well as a variety of other conditions. It is therefore one of the most important pathogens regularly encountered in the laboratory. Due to the frequency and importance of Staphylococcal infection a rapid and accurate diagnosis is needed for correct patient management, especially as the bacterium can show resistance to numerous common antibiotics. Food manufacturers find Staphylococcal detection to be a good indicator of the general level of microbial contamination. In these circumstances a highly specific test is needed to identify S. aureus, often from heavily contaminated samples. Traditional identification methods, and a number of commercially available products, may suffer from false positive results due to non-specific reactions, and false negative results due to an inability to detect certain strains of S. aureus. Microgen® Staph is a sensitive and specific test for the identification of S. aureus from primary plate culture. Also available separately is the Staph latex control reagent (product code M43CCE), helping to reinforce the accuracy and reliability of the Microgen Staph rapid test. Microgen Staph rapid test is part of a range of rapid tests which includes; Microgen C. difficile (M41CE) Microgen Salmonella (M42CE) Microgen Staph (M43CE & M433CE) Microgen E. coli 0157 (M44CE) Microgen Legionella (M45CE) Microgen Campylobacter (M46CE) Microgen Strep (M47CE) All rapid tests use latex agglutination technology.

Two of the traditional diagnostic tests for S. aureus, the tube and slide coagulase tests, only detect the activity of a characteristic coagulase enzyme. However, these tests can be difficult to interpret and are liable to suffer from false positive results associated with non-specific factors frequently found in plasma. Non-specific coagulation due to other bacterial enzymes may also occur. However, 99% of S. aureus isolates also produce detectable levels of protein A on their surface, a characteristic which can be used to differentiate S. aureus from other species of Staphylococci in which this protein is rare. Microgen® Staph is a test for the identification of S. aureus which overcomes the problems associated with the traditional tests by detecting both coagulase and protein A offering rapid and accurate identification of S. aureus in 2 minutes. Microgen® Staph contains all ancillary reagents needed to complete the test and is suitable for clinical, food and environmental laboratories.

Code M43CCE Description Microgen® Staph Control reagent Size 5 ml Storage 2 - 8°C

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MICROGEN® STREP RAPID TEST

A RAPID TEST FOR THE CONFIRMATORY IDENTIFICATION OF STREPTOCOCCI OF LANCEFIELD GROUPS A,B,C,D,F AND G FROM CULTURE PLATES

BASED ON PROVEN AND RELIABLE LATEX AGGLUTINATION TECHNOLOGY, THE

MICROGEN BIOPRODUCTS STREP GROUPING CULTURE CONFIRMATION ASSAY OFFER: RESULTS IN ONE MINUTE AFTER EXTRACTION INCREASED EFFICIENCY HIGH SENSITIVITY HIGH SPECIFICITY ACCURATE & RELIABLE RESULTS




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Microgen Strep rapid test is part of a range of rapid tests which includes; Microgen C. difficile (M41CE) Microgen Salmonella (M42CE) Microgen Staph (M43CE & M433CE) Microgen E. coli 0157 (M44CE) Microgen Legionella (M45CE) Microgen Campylobacter (M46CE) Microgen Strep (M47CE) All Microgen rapid tests use latex agglutination technology.

Code M47CE Description Microgen® Strep Size 50 tests Storage 2 - 8°C

Most strains of Streptococci which have been isolated from human infections possess serological group specific antigens. Identification of the organisms includes extraction and characterisation of these antigens from organism grown in culture. The classical method of grouping Streptococci has been the Lancefield method; however, it is very time consuming and requires considerable technical expertise. This has restricted its use to only a very few reference laboratories. The use of Latex Technology coupled with high quality, specific antisera and a specific enzyme extraction protocol has brought Streptococcal grouping within the reach of most laboratories. Microgen® Strep utilises latex particles that have been sensitised with high quality group specific antisera. Cell wall antigens are extracted by rapid enzymic digestion and then tested by a panel of latex reagents. A positive reaction is denoted by a strong agglutination reaction. The negatives remain smooth thus ensuring confidence in the final result.

Microgen Strep Features

• Rapid grouping of Groups ABCDFG

• Enzymic extraction of all groups

• Easy-to-read agglutination

• Minimal cross-reactions between groups

• High sensitivity to group D Rapid testing of Streptococcal antigens using latex reagents is a well established procedure. Release of the antigen from the cell wall can be accomplished by enzyme or chemical degradation. The enzyme reagent used in Microgen® Strep is unique.

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Microgen® GN A + B - ID

AN IDENTIFICATION SYSTEM FOR ALL CURRENTLY RECOGNISED ENTEROBACTERIACEAE AND AN EXTENSIVE

RANGE OF OXIDASE-POSITIVE BACILLI

• Convenient 12 substrate identification system for the commonly encountered Enterobacteriaceae

• Results in 48 hours • Simple and easy-to-use • Results interpreted using new Microgen-ID System Software (registered users are

entitled to free database updates)

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The Microgen® GNA-ID System is for the identification of the commonly encountered Enterobacterceae from food or medical samples including urinary samples and common wound isolates. For wider clinical applications and for veterinary and industrial samples, all currently recognised species of Enterobacterceae and an extensive range of oxidase-positive Gram Negative Bacilli can be identified using the combination of Microgen® GN A + GN B identification systems. The Microgen® GNA-ID comprises of only 12 substrates which are specifically selected to optimise the identification of the most commonly encountered oxidase negative Bacilli including the family Enterobacteriaceae and Acinetobacter spp. Other systems use 20 to 30 substrates to achieve the same levels of identification making the Microgen® GNA-ID a faster and clearer system. As with all Microgen Bioproducts biochemical ID systems, Microgen GNA + B-ID systems are supported by the simplest-to-use and most feature-packed Computer Identification System Software (MID60) available, de-livering the most comprehensive and up-to-date databases which registered users can update free-of-charge. The following organisms are included in the GNA database within the Microgen® Identification System Software:

Acinetobacter baumannii Morganella morganii Acinetobacter lwoffii Proteus mirabilis Acinetobacter haemolyticus Proteus vulgaris

Citrobacter freundii Providencia rettgeri Citrobacter diversus Providencia stuartii

Edwardsiella tarda Providencia alcalifaciens Enterobacter aerogenes Salmonella Group I Enterobacter cloacae Salmonella typhi

Enterobacter agglomerans Salmonella cholerae-suis Enterobacter gergoviae Salmonella paratyphi A Enterobacter sakazakii Salmonella gallinarum Escherichia coli Salmonella pullorum Escherichia coli - inactive Salmonella Group II Shigella dysenteriae (Group A Salmonella Group Illa Shigella flexneri (Group B) Salmonella Group Illb Shigella boydii (Group C) Salmonella Group IV Shigella sonnei (Group D) Salmonella Group V Hafnia alvei Salmonella Group VI Klebsiella pneumoniae Serratia marcescens Klebsiella oxytoca Serratia liquefaciens Klebsiella ozaenae Serratia rubidaea

Klebsiella rhinoscleromatis Yersinia enterocolitica

All of the currently recognised species of Enterobacteriaceae and an extensive range of Oxidase Positive Gram Negative Bacilli can be identified using the combination of the Microgen® GNA + GNB identification panels i.e. 24 substrates. Additional reagents available: • MID61A Nitrate A reagent • MID61B Nitrate B reagent • MID61C VP I reagent • MID61D VP II reagent • MID61E TDA reagent • MID61F Indole Kovacs reagent • MID61G Oxidase Strips • MID61H Mineral oil • MID61K PYR reagent • MID61L Ninhydrin reagent

Identification Systems available: • MID-62 Microgen Strep-ID • MID-64 Microgen GN-ID A • MID-65 Microgen GN-ID B • MID-66 Microgen Bacillus-ID • MID-67 Microgen Listeria-ID • MID-69 Microgen Staph-ID

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200608



MICROGEN® LISTERIA-ID

AN IDENTIFICATION SYSTEM FOR LISTERIA SPECIES. PRODUCT CODE MID-67 (20 TEST KIT)

♦ A complete identification system, no additional materials required ♦ Substrates included conform to all international standards ♦ Enhanced built in haemolysis test—CAMP test not required ♦ Validated for chromogenic media (BBL/AES) ♦ AFNOR validated with AES ALOA medium ♦ Inoculated directly from selective media (no need for sub-culturing before

inoculation ♦ AOAC—RI validated ♦ CMMAS Assessed and Approved



MICROGEN B IOPRODUCTS LTD

Now included in Marks and Spencer Laboratory Manual.

Improved haemolysis reaction!!

-ve Haemolysis reaction +ve Haemolysis reaction

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Listeria -ID: 12 substrates: A complete identification panel for the identification of all Listeria spp. (6 species) Code: MID-67 Description: Microgen Listeria-ID Size: 20 Test Storage: 2 –8 °C Other Identification Systems available: • MID-62 Microgen Strep-ID • MID-64 Microgen GN-ID A • MID-65 Microgen GN-ID B • MID-66 Microgen Bacillus-ID • MID-69 Microgen Staph-ID As with all Microgen Bioproducts biochemical ID systems Microgen Listeria-ID is supported by the simplest-to-use and most feature-packed Computer Identification System Software (MID60) available, delivering the most comprehensive and up-to-date databases which registered users can update free-of-charge from www.microgenbioproducts.com

Microgen Listeria-ID employs 12 standardised microwell substrates combined with the Microgen Identification System Software to identify members of the genus Listeria:

♦ Listeria monocytogenes

♦ Listeria welshimeri

♦ Listeria ivanovii

♦ Listeria innocua

♦ Listeria grayi

♦ Listeria seeligeri

The above organisms can be identified from selective agar or non-selective agar using Microgen Listeria-ID. Identification is achieved using all the tests recommended in international standard methods for the identification of Listeria spp. without the need for additional confirmatory tests.

The AOAC-RI validated Microgen Listeria-ID has optimised the differentiation of haemolytic and non-haemolytic Listeria spp. by improving the readability of the micro-haemolysis reaction, which eliminates the need to sub-culture from selective agar or perform a CAMP test. The readability of a negative haemolysis reaction has been improved; if an organism does not produce haemolysin, the red blood cells will remain intact and form a distinct red pellet. If an organism does produce haemolysin, the red cells will rapidly be lysed and cellular contents released as a red/brown solution, indicating a positive result.

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200608



MICROGEN® STAPH-ID

IDENTIFICATION SYSTEM FOR STAPHYLOCOCCI OF CLINICAL, ANIMAL HEALTH AND ENVIRONMENTAL IMPORTANCE

PRODUCT CODE: MID-69CE (20 TEST KIT) ♦ 12 test system minimises handling and manipulation ♦ Suitable for use with clinical and veterinary samples ♦ Can be used to identify isolates from food and environmental sources ♦ Substrates selected specifically for Staphylococcus and related species ♦ Simple and easy-to-use ♦ Results in 24 hours ♦ Results Interpreted using new Microgen-ID system software (registered

users are entitled to free database updates)




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Additional reagents available: • MID61A Nitrate A reagent • MID61B Nitrate B reagent • MID61C VP I reagent • MID61D VP II reagent • MID61E TDA reagent • MID61F Indole Kovacs reagent • MID61G Oxidase strips • MID61H Mineral oil • MID61K PYR reagent • MID61L Ninhydrin reagent Other Identification Systems available: • MID-62 MIcrogen Strep-ID • MID-64 Microgen GN-ID A • MID-65 Microgen GN-ID B • MID-67 Microgen Listeria-ID • MID-66 Microgen Bacillus-ID

MicrogenTM Staph-ID has been developed for the identification of commonly encountered Staphylococcus spp. isolated from clinical and veterinary samples. These bacteria, although commensal, are recognised as potential human pathogens e.g. S. aureus and S. epidermidis. Coagulase negative Staphylococcus spp. are common colonisers of prosthetic valves and in-dwelling catheters.

Gram stain (positive), catalase (positive) and latex agglutination / coagulase tests are performed as pre-tests on the isolate. Colony pigmentation is recorded and then 1-2 colonies are inoculated into the suspending medium. The suspension is inoculated into the 12 well test strip and incubated at 37°C. After 24 hours incubation the strips are read and the Nitrate and PYR reagents added. The re-sulting 5 digit numerical code is entered in to the software and an identification re-turned.

Software Features include:

• Data entry fields can be configured to suit all laboratory situations.

• Guidance in the interpretation of results.

• References to original citations and taxonomic changes.

• Results storage with full editing capabilities

• Extensive and fully maintained databases

Species Identified:

Staphylococcus aureus subsp. aureus Staphylococcus aureus subsp. anaerobius

Staphylococcus epidermidis Staphylococcus saprophyticus

Staphylococcus cohnii subsp. cohnii Staphylococcus cohnii subsp. urealyticum

Staphylococcus xylosus Staphylococcus sciuri

Staphylococcus haemolyticus Staphylococcus simulans

Staphylococcus hominis subsp hominis Staphylococcus hominis subsp

novobiosepticus, Staphylococcus warneri Staphylococcus capitis subsp capitis, Staphylococcus capitis subsp ureolyticus, Staphylococcus hyicus, Staphylococcus chromogenes, Staphylococcus intermedius, Staphylococcus auricularis, Staphylococcus

lentus, Staphylococcus carnosus, Staphylococcus schleiferi subsp schleiferi, Staphylococcus schleiferi subsp coagulans, Staphylococcus lugdenensis, Staphylococcus caprae, Micrococcus luteus, Micrococcus

lylae, Kocuria rosea, Kocuria varians, Kocuria

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11/07/2007



MICROGEN®

STREP-ID

AN IDENTIFICATION SYSTEM FOR STREPTOCOCCI AND ENTEROCOCCI FROM CLINICAL, VETERINARY, FOOD AND

ENVIRONMENTAL SOURCES.

PRODUCT CODE: MID-62 (20 TEST KIT)

♦ Substrates selected specifically for Streptococcus, Enterococcus and related species

♦ Simple and easy-to-use ♦ Results in 24 hours ♦ Results interpreted using new Microgen ID system software (registered

users are entitled to free database updates) ♦ Excellent differentiation of Enterococci spp. especially E. casseliflavus,

E. mundtii and E. gallinarum




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Microgen® Strep-ID is a biochemical test system which utilises a 12 well (12 test) microwell test strip and 3 off-strip tests; hippurate hydrolysis (provided), alpha-haemolysis and beta-haemolysis for the identification of Streptococcal and Enterococcal species important to clinical, veterinary, food and environmental laboratories. Performance Characteristics: Microgen® Strep-ID has been evaluated in comparision with a well-established commercially available product for identification of cultured bacterial isolates. A total of 69 cultures, comprising cultures from recognised culture collections and clinical isolates were examined.

The Microgen® Strep-ID correctly identified 70 (93%) and the competitor product correctly identified 52 (74%) of the isolates tested. The superior specification of Microgen® Strep-ID is complimented by an excellent differentiation of Entercocci spp. and a reduced test system minimises handling and manipulation whilst reducing procedural error and time.

As with all Microgen Bioproducts biochemical identification systems Microgen Strep-ID is supported by the simplest-to-use and most feature-packed computer identification system (product code: MID60) available, delivering the most comprehensive and up-to-date databases which registered users can update free-of-charge from www.microgenbioproducts.com Microgen® Strep-ID: Additional Reagents Available: Code: MID-62 MID61A Nitrate A reagent Description: Microgen® Strep-ID MID61B Nitrate B reagent Size: 20 Test MID61C VP I reagent Storage: 2-8°C MID61D VP II reagent MID61F Indole Kovacs reagent MID61G Oxidase Strips MID61H Mineral oil MID61K PYR reagent MID61L Ninhydrin reagent Other Microgen® Identification Systems Available: MID-64 Microgen® GN A –ID MID-65 Microgen® GN B - ID MID-66 Microgen® Bacillus - ID MID-67 Microgen® Listeria - ID MID-69 Microgen® Staph – ID

Number

T ested M ID -62 Competito r

E. avium 1 1 1

E. durans 2 2 1

E. faecalis 10 10 10

E. faecium 5 5 5

E. gallinarum 1 1 0

E. hirae 1 1 0

G. haemolysans 1 1 1

S. acidominimus 1 1 0

S. agalactiae 5 5 5

S. anginosus 3 3 2

S. bovis 1 1 1

S. constellatus 3 2 3

S. dysgalactiae ssp. equi 1 1 1

S. equi subsp. equi 2 2 2

S. equi subsp.zooepidemicus 1 1 1

S. gordonii 1 1 0

S. intermedius 1 1 0

S. mitis 9 9 5

S. mutans 4 3 4

S. parasanguinis 1 1 0

S. pneumoniae 5 4 2

S. pyogenes 3 3 3

S. salivarius 4 4 3

S. sanguinis 2 0 2

S. uberis 1 1 1

S. vestibularius 1 1 0

TOTAL 70 65 53

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PATH-CHEK HYGIENE PATHOGEN

SYSTEM

RAPID TESTS FOR THE DETECTION OF PATHOGENIC BACTERIA FROM WORK

SURFACES OR PROCESSING EQUIPMENT

• HIGH LEVELS OF SENSITIVITY (<1 CFU PER 10CM2) AND SPECIFICITY

• COMPLIANT WITH THE REQUIREMENTS OF ISO 18593:2004(E)

• MEETS THE REQUIREMENTS OF USDA/FSIS AND US FDA TESTING METHODS

• PRE-MOISTENED SWABS INCREASE RECOVERY FROM WET AND DRY SURFACES, NEUTRALISES RESIDUAL DETERGENTS AND SANITISING AGENTS AND PRESERVES ORGANISMS.

• SPECIFIC PATHOGEN DETECTION BROTH AVAILABLE FOR LISTERIA, COLIFORMS AMD SALMONELLA

• CONVENIENT AND EASY-TO-USE

• NO NEED TO TAKE INCUBATION MEDIA INTO PRODUCTION AREA

Phone: +44 (0)1276 600081 Fax: +44 (0)1276 600151 E-mail: [email protected] www.microgenbioproducts.com



Salmonella Listeria Coliforms

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As food manufacturers and processors realise the importance of microbiological environmental hygiene monitoring as part of a good manufacturing practices in the food industry, the need for a cost-effective, easy-to-use, rapid and accurate method for environmental sampling has become increasingly more important. Microgen Bioproducts Ltd has met this need by developing a new range of environmental pathogen detection systems: Path-Chek Hygiene Pathogen Systems. These systems meet all of the requirements of ISO Standards for environmental sampling (ISO 18593:2004(E)) through the use of pre-moistened swabs for the sampling of surfaces, combined with organism specific chromogenic detection broth into which the swab is placed and incubated at the appropriate temperature.

The range of Path-Chek Hygiene Pathogen Systems consist of tests for 3 organism types; Listeria, Coliforms and Salmonella.

All three systems utilise an identical test principle. Bacteria are recovered from the surface of food manufacturing or processing equipment using a pre-moistened swab, which is capable of neutralising commonly employed detergents and sanitisers. The sample swab is then transferred into an organism specific media (swabs have a conveniently located break point so that they can easily be broken off into the growth media). The test media are then incubated at 35-37°C for 18-24 hours for Coliforms and Salmonella, and at 28-30°C for 24 -48 hours for Listeria (If Path-Chek Hygiene Listeria is incubated at 35-37°C, there will, in certain circumstances, be an increased risk of false positives). If the specific target bacteria are present on the sample swabs, bacterial growth will cause the colour of the media to change.

Test reactions:

Coliform Results: Positive Negative Salmonella Result: Positive Negative Listeria Result: Positive Negative

Microgen Path-Chek Hygiene Swabs Pack Size 100 swabs Storage Room temperature

Microgen Path-Chek Hygiene Pathogen Detection Broth Pack Size 100 Storage 2-8°C Listeria PC080 Salmonella PC020 Coliforms PC010

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PATH-CHEK™ HYGIENE

PROTEIN SWAB

SIMPLE AND RAPID TEST FOR THE

DETERMINATION OF THE LEVEL OF

SURFACE PROTEIN IN FOOD HANDLING

ENVIRONMENTS

Phone: +44 (0)1276 600081 Fax: +44 (0)1276 600151 E-mail: [email protected] www.microgenbioproducts.com



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The increased awareness of the need for high levels of hygiene in food handling and processing environments combined with the widespread implementation of HACCP Programs has resulted in the need for simple and effective methods for the rapid assessment of hygiene and the effectiveness of cleaning programs. PathchekTM Hygiene is a chemical test based on the detection of protein, a common component of most food types. This test is not targeted specifically at bacteria. When cleaning procedures are not effective or have been performed poorly, both food residues and bacteria will be present and will result in a positive reaction. PathchekTM Hygiene is provided in a simple peel open plastic pouch which contains a pre-moistened swab for sampling test surfaces. After sampling, the swab is returned to the pouch and pushed down into the developing reagent. If positive (indicating the presence of food residues and bacteria), a colour reaction (green to blue) will develop within 5 seconds.

Path Chek Procedure:

Code PC005-100

Description PathchekTM Hygiene

Size 100 tests

Storage Below 30°C

Peel open

the plastic

pouch

Remove

the mois-

tened swab

Swab the

surface to

be tested

Return the

swab to the

pouch

Push the

swab down

into the

developing

reagent

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ROTAVIRUS SCREENING

Phone: +44 (0)1276 600081 F ax: +44 (0)1276 600151 www.microgenbioproducts.com

1 Admiralty way, Camberley, Surrey GU15 3DT


M480CE Rotascreen® EIA, 96T • Detects the presence of rotavirus in under 1 hour. • Can be used in parallel with Adenoscreen® EIA. M80CE Rotascreen® Latex, 50T • Sensitive and specific, with a result in 5 minutes. • No special equipment required. •Can be used in parallel with Adenoscreen latex using same faecal sample preparation.

M580CE - Rotascreen® Dipstick 25T New, improved Rotascreen Dipsticks! • Fast results in 5 minutes • Superior monoclonal antibodies • Improved lateral flow technology M585CE - Rota/Adenoscreen Duo Cassettes 25T • Fast results in 5 minutes • Cassette format improves simplicity of test • Sample preparation unit eliminates need for centrifugation or filtration of sample.

Improved Rota/Adenoscreen dual dipsticks now available in cassette

format!

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ADENOVIRUS SCREENING




M481CE Adenoscreen® EIA, 96T. • Detects presence of adenovirus in under 1 hour.

• Can be used in parallel with Rotascreen® EIA

M81CE Adenoscreen® latex, 50T • Results can be obtained within 5 minutes. • Based on detection of hexon antigen allows Adenoscreen® to detect all types of Adeno virus

• Can be used alongside Rotascreen® latex using the

same faecal sample preparation.

M583CE - Rota/Adenoscreen Dual Dipstick 25T New, improved Rota/Adenoscreen Dipsticks! • Fast results in 5 minutes • Superior monoclonal antibodies • Improved lateral flow technology M585CE - Rota/Adenoscreen Duo Cassettes 25T • Fast results in 5 minutes • Cassette format improves simplicity of test • Sample preparation unit eliminates need for centrifugation or filtration of sample.

Improved Rota/Adenoscreen dual dipsticks now available in

cassette format!!

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HELICOSCREEN




A rapid lateral flow immunoassay for the detection of H. pylori antigen in faeces

Helicoscreen Rapid Cassette detects H. pylori antigen from faecal

samples: • Simply: cassette format and one-step procedure. • Rapidly: results within minutes. • Controls: each cassette comes with a built in control line. • Ease of Reading: clear distinct reaction lines with a clean

background. • Reliably: high sensitivity (98.5%) and specificity (98.1%).

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Helicoscreen is a sensitive and specific rapid cassette test which detects H. pylori antigen in faecal samples within minutes. Helicoscreen complements the range of rapid cassettes for the detection of infectious agents responsible for gastro-enteric diseases from Microgen Bioproducts. The company’s range includes rapid tests for the leading causes of gastro-enteritis; Rotavirus, Adenovirus and Norovirus, allowing for a laboratory to test in-patients exhibiting symptoms of diarrhoea and vomiting rapidly with sensitive and specific tests, allowing for the correct treatment to be implemented as well as patient isolation policy. Helicoscreen is an easy-to-use cassette which includes a sample preparation device:

1. Remove sample probe from collection device and coat liberally with sample. 2. Replace probe and shake to disperse material. 3. Snap top from collection device and invert vertically. 4. Add 3 drops (120 µL) to the sample well of the cassette. 5. Leave at room temperature and read the results after 10 minutes. Strong

positive results will be observed sooner. Performance Data:

Sensitivity = 98.5% (94.6%-99.8%) Positive Predictive Value = 97.7% Specificity = 98.1% (94.2%-99.6%) Negative Predictive Value = 98.7% Overall agreement = 98.3% (96.0% -99.4%)

Product Code Description Kit Size

M586CE Noroscreen Cassette 10

M585CE Rota/Adenoscreen Cassette 25

M587CE Helicoscreen Cassette 25

Endoscopy

+ - Total

H. pylori antigen raid test

+ 130 3 133

- 2 151 153

132 154 286

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Documents

CATÁLOGO MICROGEN MICROBIOLOGÍA 2013, AKRALAB