Capillarys 2 Technical Manual

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Capillarys 2 Technical Manual

December 2008

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SUMMARY Page

Section 1 What’s new on the mechanics? ........................................................ 3

Section 2 What’s new on the hydraulics? ......................................................... 3

Section 3 What’s new on the optics? ................................................................ 4

Section 4 What’s new on the electronics? ........................................................ 7

Section 5 What’s new on the main menu? ........................................................ 10

Section 6 What’s new on the service menu? .................................................... 11

Section 7 Other system calibrations ................................................................. 21

Section 8 Where are saved all the calibrations ................................................ 27

Section 9 New error codes and known errors on Capillarys 2 ........................ 29

Section 10 Capillarys error log files .................................................................... 30

Section 11 Firmware update ................................................................................. 34

Section 12 Cycle timing on Capillarys 1 & Capillarys 2 ..................................... 36

Section 13 Precautions when dismantling the system ...................................... 36

Section 14 Recommended spare parts ............................................................... 37

Section 15 Capillarys principle (reminder).......................................................... 38

Section 16 Electronics, hydraulics & optics bloc diagrams and schematics .. 40

Section 17 Troubleshooting the system, warning & error codes ..................... 52

Section 18 Installation, operator maintenance & PM check-lists ...................... 54

Section 19 Instrument calibration sheet ............................................................. 65

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What’s new on Capillarys 2 compared to Capillarys 1?

4 main objectives: more flexibility to run the new assays, simplify the maintenance, improve the accessibility and dismantling of the different parts, improve the reliability. 1) What’s new on the mechanics? Smooth cover surface dark grey and silver grey, Interlock for the introduction of the sample racks with a shutter, 2 vertical pins

on the inlet conveyor and a “hand presence” sensor (capacitive level sensor) on the front cover to prevent any jam. The presence of the hand close to the sample rack entry preventing the rotation of the inlet conveyor belt,

Retroactive adjustment of the lateral play on the sample rack holder as soon as it has been loaded,

Inlet and outlet conveyor without grooves for the belts (easier to clean and better gap for the sample racks moving on the conveyor),

Better access for the adjustment of the belt tension on the lateral movement (motor 9). The adjustment being easily accessible on the left instead of the right underneath the tank 2,

New encoder wheel (same as the Hydraplus) for the motor 9, Improvement of the sample probe X & Y movements: new endless screw on

the vertical movement (more torque), flat on the 2 vertical axis guides, magnetic encoder wheel on the vertical movement, new mounting of the horizontal endless screw,

New hinge and reinforcement of the front cover and new shape of the interlock to close the cover easily,

No more hinge on the rear cover, Reagent compartment lid attached to the right cover, Top cover of the tank 2 made of epoxy (to make it more rigid), New fastening of the thermal bridge on the capillaries (quick coupling), Double noise insulation for the pump with rubber shock absorbers, New wiring underneath the conveyor, New fixing of the anode on the high voltage and new fixing of the 4-way

valves.

2) What’s new on the hydraulics? New hydraulics more simple thanks to the use of three 4-way valves and

1manifold: 6 meters of tubing instead of 18, Only 1 pump for pressure and vacuum and 1 reservoir (pressure) instead of 2, Use of 2 ceramic 4-way valves (+ 1 optional) to reduce the number of valves.

Note that the 4-way valve on Capillarys 1 is a Teflon type, New air and liquid valve models with connectors, New tank 2 design with only one reagent entry to prevent any buffer

contamination by the wash and/or the water, Better injection: injection vacuum made by the syringe and controlled by the

vacuum sensor (Capt. 2), little over pressure before injection to reduce the false injection by capillarity due to the vertical movement of the dilution segment under the capillaries,

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New Teflon coated and not shielded sample probe like the one on Hydraplus (consequently there is no more floating ground for the sample probe assembly),

New washing block with an O-ring to drive the needle vertically and valve (EV17) directly attached on the block,

Liquid valves mounted directly on the tank 1 port bracket (no more valve board),

New pressure reservoir combined to a manifold and an adjustable pressure regulator for the reagent containers,

Optional 4-way valve (not connected) for multiple buffer connection, Gutter to evacuate the liquid in case of leak, Overflow of the tank 2 through the valve EV 21 and the gutter (not into the air

circuit), Draining of the pressure reservoir through the tank 2 and the valve EV 21, Purge of the pressure reservoir through the safety valve located at the very

bottom when shutting down the system, New design of the tank 1 to let the liquid escape to the gutter in case of

overflow,

tank 1 with a notch on the side Control of the tank 1 and 2 draining through the same opto without any acrylic

(Plexiglass) adapter for the tubing (no more adjustment by polishing the inner part of the tubing adapter to get the right amount of light on the opto).

3) What’s new on the optics? Better signal-to-noise ratio, Use of a monochromator to work at different wavelengths from 190 to 600 nm, Definition A monochromator is an optical subassembly that is used to isolate narrow portions of the spectrum. It accepts polychromatic input from a lamp or laser and outputs monochromatic light.

On Capillarys, the polychromatic light enters via the 1 mm slit of the deuterium lamp. Inside the device, the dispersive element, a grating here, (it could be also a crystal prism or a mirror) diffracts the light into its spectrum. The grating being rotated by a motor. The angle at which the grating is rotated determines the wavelength of the output monochromatic light, and determines the specific color of the light.

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unpolished (frosted) UV glass surface + emission fiber optics

Deuterium lamp (with a 1 mm slit) Motor (stepper motor #14)

Unpolished UV glass surface + emission fiber optics

deuterium lamp (lamp 24W with a slit of 1mm) + lamp shutter (motor #15) WARNING: never touch or clean the diffraction grating. Even if it is a little bit dusty, it won’t have any effect on the results.

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1 - Deuterium lamp, 2 - Lamp shutter 3 positions (closed, open, UV cut-off), 3 – Lamp shutter motor, 4 - Monochromator, 5 - Home position opto sensor, 6 - Monochromator motor, 7 - Unpolished (frosted) UV glass surface, 8 - Input fiber optics bundle, 9 - Reference fiber optics, 11- Capillary, 12- Reference fiber optics coupling, 13- Output fiber optics bundle, 14- Linear image sensor 256 pixels (CCD)

15- UV filter (glass window for wavelengths over 350 nm used when running hemoglobin samples at 415 nm).

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New lamp shutter with 3 positions: closed, open and a third one with a glass window to block the UV under 350 nm. It is used when running Hb samples at 415 nm,

Glass fiber optics without quarter-turn fastening and reference fiber optics without attenuator (no more adjustment). It means that all the fibers are now the same,

Use of an unpolished (frosted) UV glass surface on the common point of the emission fiber optics to have a better light homogeneity on the 9 fibers.

4) What’s new on the electronics? New CCD barcode reader with a better resolution (same as the one used on

few Hydraplus and Hydraplus 2 with a resolution of 0.1 mm.). Note that the barcode setting can be changed by scanning configuration barcodes (not from the configuration screen on the Capillarys software),

Better control of the temperature with the use of a new temperature probe having a resolution of 0.1°C. Consequently, the Distributor board is a little bit

different: the resistors R2 and R3 have been removed (10 K previously) and

R11= 0 (instead of 56.2 K ), Note that the new temperature probe can be installed on Capillarys 1 with an upgraded Distributor board,

Reset of the 3 major boards (CCD, Passeur/Sampler and Distributor) at 4.5V instead of 4.75V to prevent some intermittent false errors 060x encountered on Capillarys 1,

All the flat connectors are keyed with the indication of the board, the connector and also the name of the sensor (i.e. CAP.17 and X-Y. J7) for the vertical home position of the sample probe),

Opto sensors with a LED and a connector (no more flat cable to make them interchangeable what ever the distance between the sensor and the board),

Liquid sensor for the filling of the tank 2 (photo transistor Capt..4) and for the draining of the tank 1 and 2 (opto #5) work now in modulated light (better immunity against parasite light such as the sun light of artificial light in the room),

High voltage power supply with a removable flat cable on the small interface board,

CCD power supply is grounded, Electronic board overview:

Existing boards on Capillarys 1 CCD board (CCD.): unchanged except a capacitor on the quartz output for the CE mark, has also a new reset threshold at 4.5V instead of 4.75V. Sampler/Passeur board (PAS.): unchanged except a capacitor on the quartz output for the CE mark, has also a new reset threshold at 4.5V instead of 4.75V and is grounded to the chassis. Distributor board (DIS.): for the new temperature probe, the resistors R2 and

R3 have been removed (10 K previously), R11 is equal to 0 (instead of

56.2 K ), has also a capacitor on the quartz output for the CE mark, a new reset threshold at 4.5V instead of 4.75V and is grounded to the chassis.

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X-Y sample probe motor board (X-Y.): unchanged except connector J1 which can be disconnected. Sampler (and barcode reader) interface board (CHA.): unchanged except the connector J1 which can be disconnected. Syringe board (SER.): unchanged except the connector J1 which can be disconnected. Inlet conveyor board (ENT.): unchanged except the connector J1 which can be disconnected. Note that the board is located now on the outlet conveyor side. Boards which are not existing anymore Safety board with the buzzer, Capillaries board underneath the tank 2, Valve board with the air and liquid valves, Outlet conveyor board. New boards Rack introduction board (INT.): it has the same function as the Outlet conveyor board on Capillarys 1 but has also to manage the interlock system (motor 16 and its opto Capt.10) for the introduction of the sample rack with the “hand presence” sensor (capacitive sensor Capt. 39). It is connected to the 24V and the I2C bus. Tank board (CUV.): interface board for the liquid valves EV11, EV12, EV13, EV15, EV21, the Peltier fans, the Peltier temperature probe, the front cover interlock, the common draining sensor for the tank 1 and 2 (Capt.5), the filling sensor for the tank 2 (Capt. 4), the 4-way valve EV16 (motor 13 and its opto Capt.7), the lamp shutter (motor 15) and the monochromator (motor 14 and its opto Capt.9). It is connected to the Distributor board and the I2C bus. Note that it is the board with the buzzer and that the lamp shutter motor does no have a home position opto. The shutter motor being a stepper motor with only 20 steps to cover the opening angle for the 3 positions, it is then supplied with 20 steps counter clockwise to close the light (home position) when starting the system. Reagent container board (BID.): it is in charge of the 4 container liquid level sensors to warn the operator when they have to be replaced: blue light when the level is fine, red when the reagent container is empty or full for the waste. It is connected to the 24V and the Distributor board. The mid level sensor being used to readjust the instrument calculated level (as of rel. 5.15).

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Instrument SN 401 to 700 waste container full sensor (photo diode) mid level sensor

blue and red lights (LEDs) reagent container empty sensor

(photo diode) Valve interface board (ELE.): interface board for the air valves EV1, EV2, EV3, EV 5, EV7, the pressure and vacuum sensors Capt.1 and Capt.2. It is connected to the new Reagent container board. Opto sensors The opto sensors are the same as the ones used on Capillarys 1 but the current has been increased of 20% in the infra red diode to get a better detection.

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5) What’s new on the main menu?

Enter the service password 46448630, click on the new button and select Capillarys 2 Note: It is possible to switch from Capillarys 1 to Capillarys 2 as of the software version 5.17. The versions 5.11, 5.12 and 5.15 were dedicated only to Capillarys 2.

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6) What’s new on the service menu?

Distributor - Passeur/Sampler tests

Check the box of the part you want to control to refresh the data

New motors

Note: the motor 16 being a DC motor and not a stepper motor. It is better to check it with Start rack introduction (M16) The motor 15 (Deuterium lamp filter selection) can be checked as well from Open-Close Beam source (10 steps to reach the open position and 10 more

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steps to reach the glass filter position, 20 steps backward to return to the home position).

Click on page 2 to see the new sensors Note: the sensors Capt.33, 34 and 35 do not exist anymore, Capt.32 does not exist as well like on Capillarys 1.

CCD - optical setting

This dialogue window is used to check the optics and align the reception fiber optics on the CCD like on Capillarys 1 (there is no more alignment of the emission fiber optics) by clicking on Open Beam Source and setting the integration time. It is also used to calibrate the monochromator when required only.

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Check the box RESPONSE CURVE, click on START Scan to get the response spectrum of the optics (deuterium lamp + monochromator + fiber optics + CCD) and calibrate the monochromator stepper motor (motor 14). The deuterium lamp having some special characteristics, we use the visible Balmer line at 656.3 nm it to calibrate the monochromator. The deuterium being an atom of the hydrogen, see hereafter the hydrogen spectrum with the visible Balmer lines.

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Hydrogen Spectrum

Peak used to calibrate the monochromator at 656.3 nm. The home mechanical position of the monochromator is given by the sensor #9 (close to 700 nm). Warning: The gear box on the monochromator motor having a ratio of 500, it is very easy to break that„s why it is not recommended to play with the Start, Stop and Direction commands of the motor 14. A new calibration has to be performed only when required. Set the integration time to have enough signal and prevent too much saturation (around 12 ms). The amplitude of the small peak at 656 nm should be around 0.2 to 0.3 V to have a good detection of the peak (not a spike) and then a good calibration of the monochromator motor. Starting the rotation from

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the home position at approximately 700nm when starting the scan, it is the first peak detected by the CCD board.

number of steps of the monochromator motor

The system will display successively the number of steps of the monochromator motor and the wavelength in nm.

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wavelength An error in nm is displayed for the 3 typical wavelengths (0, 200 and 415 nm) according to the theoretical curve of the monochromator. The 0 nm (zero) being the middle of the saturated peak on the right of the spectrum curve when the grating position is like a mirror returning all the wavelengths of the lamp. From the 3 errors, a correction (calibration) of the monochromator motor steps is calculated and saved on the EEPROM of the Distributor board. Note that this calibration is not visible in the customized parameters (modified parameters.ini file). Note: The response curve of the optics which is not the calibration of the monochromator can be stored on the PC (into C:\Program file\Phoresis\Capillarys\Log folder) with the Save button. A saved curve can be displayed as well with the Load button.

WARNING: Never touch or clean the diffraction grating. Even if it is a little bit dusty, it won’t have any effect on the results.

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Cycle tests

New test cycles C28 and C29.

Note: The cycles C17, C18, C24 and C25 are not applicable on Capillarys 2 (only on Capillarys 1) and the cycle C20 is the same as before on Capillarys 1, it has just been renamed as Capillary clogged test with pressure. The cycle C26 is used only in the factory.

Cycle test C16 - Dilution cycle 3.0

The test has been improved to check the capacitive level detection on the sample probe (DCN board). The 8 dilutions can be checked by clicking on the corresponding box on the left.

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It shows the signal (rising edge) on the DCN board when the needle senses the liquid for the 8 dilutions.

Cycle test C28 - Calibrate rack introduction card (hand sensor) It is fully automatic, there is nothing to do. Avoid putting your hand or your body close to the conveyor cover to prevent any false calibration. It consists of calibrating the hand presence sensor (capacitive sensor with the metal plate) located on the cover. Note: The expected value (threshold) is around 4000 to 8000 (it could be higher as the soon as there is a rising edge) and is saved on the Rack introduction board. In case of wrong calibration, the sample rack won‟t move on the inlet conveyor to be processed. The purpose of the calibration consisting to prevent any jam on the inlet conveyor by blocking the rotation of the belts until the hand of the operator has been removed from the loading entrance of the compartment.

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Note: The threshold value is displayed on the Distributeur-Passeur test window:

Cycle test C29 - Calibrate container card Fill the 4 containers, close the covers and follow the instructions.

Warning: Don’t forget to drain the waste bottle when the calibration has been performed.

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Note: The container board is used to warn the operator when a container has to be replaced. In that case the container is red. It is used also to correct the calculated volume by the system with the mid sensor on the 4 containers (as of version 5.15). The calibration is saved on the Container board.

The threshold values are displayed on the Distributeur-Passeur test window to be compared with the values corresponding to the current level inside the containers:

waste water

wash buffer

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7) Other system calibration DCN board calibration (unchanged)

Eppendhorf tube filled with 100µl of H2O a) Place an empty sample tube of 5 ml in position 1 on a sample rack with

an Eppendhorf tube filled with 100µl of H2O at the top of the tube as shown on the picture,

b) Select Cycle 27 - Calibrate DCN card from the service menu when the

system is Ready (immediately after priming). Start the cycle and insert the sample rack,

c) When the sample rack is introduced, click on Sensor calibration. The

calibration taking approximately 1 min.

d) When it is finished, the system displays the calibration values (see hereafter the accepted range for the Gain PGA, the Threshold and the Offset),

e) Check the values and click on Save parameters if there are within the

range,

f) If the values are not correct, make a new calibration. If after 5 trials, there are still outside of the range, check the tube, clean up the sample probe extremity, check the sample probe connections, the good insulation between the chassis and syringe assembly, the vertical movement of the probe, the washing block…

DCN Calibration values

Gain Gain PGA usually 4 Dephasage Threshold within 400 (800 before) and 4000 Offset within 800 and 8000 Coefficient

Important: it is highly recommended to make a cleaning cycle of the needle with Capiclean and the sample rack #100 before performing this calibration. Same thing as well with a brand new needle. The DCN calibration is saved on the DCN board.

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Motor 5 calibration (unchanged) Home position of the probe: adjust the sample probe extremity in the middle of the washing block like on Capillarys 1 make the adjustment with the opto-sensor or the needle itself. In the dilution segment: on the software versions previous to the 5.17, customize the parameter P70 to have approximately 2 to 3 mm of the needle extremity into the liquid when it fills the cup up to 200 µl. sample probe washing block

sample probe dilution segment

2mm 200 µl inside the cups Since the software version 5.17, the calibration is fully automatic with the sample rack #100 and an IT segment in place with 380 µl of liquid in the first cup (double cup). Select the option Calibration of the needle, the calibration consists for the system to sense the liquid with the needle extremity to determine the height of the liquid then no need anylonger to customize the parameter P70.

Motor 6 (forward/backward movement of the needle) calibration (unchanged) Customize the parameter P69 to center the needle in the dilution segment cups (10 motor steps corresponding to approximately 1 mm). To save the new setting, it is important to customize it from the Ready mode.

Note: The calibration is saved on the Distributor board and indicated on its cover (P69

setting).

Motor 11 (sample rack ) calibration (unchanged)

Customize the parameter P43 to center the needle in the dilution segment cups. To save the new setting, it is important to customize it from the Ready mode.

Note: The calibration is saved on the Distributor board and indicated on its cover (P43

setting).

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Barcode reader alignment and setting The light beam being wider on the sample rack and tubes with the new CCD barcode reader, there is theoretically no alignment. In case of bad reading of the sample rack barcode, customize the parameter P50 from the Ready mode. It is the same principle as the parameters P49, 51 and 52 when calibrating the motor 9 (10 motor steps corresponding to approximately 1 mm). Regarding the barcode reader setting itself: changing the check digit control for instance, contact Sebia France to get a configuration sheet to print and scan manually from the service menu (barcode reader On). In that case, the parameter P53 has to be customized first from 1 to 0 to prevent any reset of the barcode reader to the default factory setting.

Note: The code 39 setting can not be changed because it is the one used on the sample racks (# 0 to 100 + Sebia rack). For the other types, it is possible since the software revision 5.21.

Reagent container pressure calibration

From the Distributor/Passeur test screen, fill the pressure reservoir to 2000 mb then open the air valve EV 3. Measure the pressure with a manometer after the pressure regulator and set the pressure to 400 mb/4.8 psi by pulling first the knob to the left to turn it. Regarding the safety valve, it is set to 1 bar in the factory. In case of wrong setting over 400 mb, you will have to restart from the atmospheric pressure to increase it slowly up to 400mb.

pressure manometer to be plugged on the output of the pressure regulator

4- way valve (EV16, EV 18 & EV 19) adjustment after dismantling The valve opto sensor can be adjusted a little bit to get a better flow (for instance on the valve EV16 to fill the tank 2 with a new reagent filter).

Note: The home position of the valve is the port facing the flat on the motor axis.

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There are 180 motor steps between 2 ports (90°) on EV 18 and EV 19 (optional) and 360 on EV 16 (half-step command).

Volume in the tank 1 (unchanged). Adjust the electrode of the capacitive level probe to have 5 ml into the tank 1: Fill the tank with H20 from the test cycle C08 and measure the volume with a plastic syringe.

Empty the tank 1 with a plastic syringe to measure the volume

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Adjust the electrode of the capacitive level sensor vertically if necessary to get 5 ml.

Renew the test if not correct until getting the right adjustment. In case of bad adjustment, the calculated volume of buffer and H2O consumption will be wrong.

Note: There is no adjustment at all for the tank 2., the level being detected by the photo detector Capt. 4.

Motor 9 alignment (unchanged)

Click on Mechanical settings to control or calibrate the 3 positions of the motor 9 (sample rack holder) after any shipment or dismantling of the system:

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Note: The 3 calibrations are saved on the Distributor board and indicated on its cover (P49,

51 and 52 setting).

Optical alignment

Click on CCD - Optical settings to center the reception fibers on the CCD after dismantling or replacement of the CCD board or reception fiber optics

8) Where are saved all the calibrations

Calibration Location

Monochromator

Distributor board only

DCN (sample probe capacitive detection)

DCN board

Reagent container

Reagent container (bidon) board

Rack introduction (hand sensor)

Rack introduction (portoir) board

Motor 6 (P69) Distributor board on Capillarys and into Machine customized parameters (modified parameters.ini) on PC

Motor 9 (P49, P51, P52) and P50 for the barcode reader

Distributor board on Capillarys and into Machine customized parameters (modified parameters.ini) on PC

Motor 11 (P43) Distributor board on Capillarys and into Machine customized parameters (modified parameters.ini) on PC

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Machine customized parameters (from Capillarys\Configuration\System configuration…\Technical parameters)

Modified parameters.ini file (from C:\Program files\Phoresis\Capillarys) [MODIFIED_PARAMETERS_LIST] P20= P43=540 (5 means + +40 motor steps) P49=54 (5 means + +4 motor steps) P51=62 (6 means - -2 motor steps) P52=55 (5 means + +5 motor steps) P69=56 (5 means + +6 motor steps) Note: When there is no calibration or something different between the calibration saved on the Distributor board and the PC, the system will display one of the 3 following warning messages: 0x0527=Capillarys does not have any customized parameters. For security, we highly recommend to check the customized parameters as soon as Capillarys will be in the state 'Ready'. 0x0528=The serial number of Capillarys connected to your computer has changed since last connection. For security, we highly recommend to check the customized parameters as soon as Capillarys will be in the state 'Ready'.

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0x0529=Your PC does not have any customized (modified) parameters to send to Capillarys. For security, we highly recommend to check the customized parameters as soon as Capillarys will be in the state 'Ready'. 9) New error codes on Capillarys 2 (see Section 17) and Known errors on (May 2005)

0X0195=SAMPLER - Motor 5: Absence of CDT diluent.

0X01a9=SAMPLER - Motor 9: No detection of carrier movement (shift) from the home position sensor.

0x0020=CCD - Entered integration time too long for calibration with the monochromator.

0x0021=CCD - No detection of the peak at 655 nm.

0x0054=CCD - I2C link buffer saturated.

0x0070=CCD - No detection of the monochromator home position.

0x0071=CCD – Movement Timeout from one of the Tank board motors.

0x0072=CCD - Unknown error from the Tank board.

0x0073=CCD - Tank board motors not initialized.

0x0074=CCD - One of the Tank board motors is already in progress.

0X0278=Error in the instrument customized parameters.

0X0280=Unknown I2C link request.

0x8281=DISTRIBUTOR - Tank board initialization incorrect.

0x8282=DISTRIBUTOR - No detection of the reagent 4-way valve home position (EV 16 connected to the tank 2).

0x8283=DISTRIBUTOR - Motor movement impossible on the Tank board, another motor being already in progress.

0x8284=DISTRIBUTOR - Tank board motor not connected during a motor movement request (motor or 4-way valve EV16).

0x8285=DISTRIBUTOR - Motor movement impossible on the Tank board during a motor movement request (motor or 4-way valve EV16).

0x8286=DISTRIBUTOR - Time out to stop the Tank board motor on home position sensor.

0x8287=DISTRIBUTOR - Time out motor step movement on the Tank board.

0x8288=DISTRIBUTOR - Unknown error from the Tank board.

0x0290=DISTRIBUTOR - Wrong reception of the I2C link response.

0x0301=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 after clearing of the home position with the motor 16.

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0x0302=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 when trying to stop the motor 16.

0x0303=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 when trying to stop the motor 16 on home position.

0x8289=Sound file error from the Tank board.

0x8193=SAMPLER - Sound file request error for the Tank board buzzer.

0x81a5=The sample probe height calibration for the IT dilution has failed (no liquid detected or detection out of range).

0x81b5=The sample probe height has not been calibrated for the IT dilution. The height will be set to the default position given by the parameter #136.

0x81c5=Register reading error of the motor 5 encoder steps.

Known errors on Capillarys 2 (May 2005) Stop on the 7th dilution or at anytime: click on the Abort button in the status

window to return to Ready. 0x0210=DISTRIBUTOR - Pressure: Rising time too long check the reagent

containers (air leak). 0x0239=DISTRIBUTOR - Tank 1 full detected by the sensor 3 after draining

request of the tank check the waste container (air leak), 0x023A=DISTRIBUTOR - Tank 2 full detected by the sensor 4 after draining

request of the tank check the waste container (air leak). Errors during the shutdown: the system goes back to the Ready mode when

the error has been fixed and validated. Intermittent and curious errors with the detection of the home position on the

motors (motor blocked, home position not detected) After investigation, it is due to the opto which is not sensitive enough to detect the home position of the motor. It is even more curious since we are using exactly the same optos as the ones used on Capillarys 1 and such problem has never been reported. The solution consists to increase a little bit the current into the opto emission diode (infrared diode) of the faulty sensor by changing the resistor R1 from 470 or 390 ohms to 330 ohms (see attached file). Note that all the opto sensor boards have been modified since the S/N 449.

0x0054=CCD - I2C link buffer saturated encountered on the versions 5.14 and 5.15 after a standby of the instrument or after switching from one technique to another. Turn off the system and restart. Note that this error has been fixed on the version 5.17.

Note: The Self-diagnostic test in the Special cycles menu is not validated on Capillarys 2 (Capillarys 1 only). It gives the error 0x0218=DISTRIBUTOR - Vacuum sensor error.

10) Capillarys error log files

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Select respectively Capillarys\Service…\View log file, enter the password SebiaLogFile, then open the current log file or another log file.

See hereafter an example from the day 26 in 2007 (Log_day_26). The log files are saved into C:\Program files\Phoresis\Capillarys\Logs. It is very useful to see all the errors displayed in red and make sure that the system has been shutdown properly at the end of the day.

Type Date Time Message

info 01/26/07 14:26:35 Capillarys shutdown done

info 01/26/07 14:13:32

Phase MP_Shutdown: OUT level = 56456 µl (5%) H2O level = 393094 µl (56%)

NaOH level = 252000 µl (36%) BUFFER level = 441174 µl (63%)

info 01/26/07 14:13:32 Shutting down Capillarys ...

info 01/26/07 14:13:32 Inactivity period : 2:13:06

info 01/26/07 12:00:25 ** Ready **

info 01/26/07 11:58:05 Black calibration done (duration :

0:00:09)

info 01/26/07 11:57:55 Starting black calibration ...

info 01/26/07 11:57:55 Calibration done (duration : 0:00:34)

info 01/26/07 11:57:20 Starting calibration ...

info 01/26/07 11:57:20 Priming done (duration : 0:05:11)

info 01/26/07 11:52:09 Starting Priming ...

info 01/26/07 11:52:09 Capillarys initialization done

info 01/26/07 11:52:03

Phase MP_Initialise: OUT level = 10500 µl (1%) H2O level = 427000 µl (61%)

NaOH level = 252000 µl (36%) BUFFER level = 448000 µl (64%)

info 01/26/07 11:52:03 Initializing Capillarys ...

info 01/26/07 11:48:47 Opening communication port ...

error 01/26/07 11:37:41 Error during instrument status request{

(Code = 0x1102) Time out on Capillarys

received message.}


error 01/26/07 11:37:41 STOP IN COM ERROR AFTER MAX RETRY :

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Timeout on Capillarys receveid message.

info 01/26/07 11:31:15 ** Ready **


debug 01/26/07 11:31:13 Capillarys DLL Initializing ...

error 01/26/07 11:30:43 STOP IN COM ERROR AFTER MAX RETRY :

User stopped the connexion with

Capillarys.


info 01/26/07 11:30:27 ** Ready **




info 01/26/07 10:26:56 ** Ready **


0:00:09)




info 01/26/07 09:56:23

Changed parameters : - [P154] : 8 -> 6 (Temps d'intégration

Min.) - [P169] : 7 -> 8 (Définition tampon

utilisé)

- [P240] : 300 -> 360 (Nb points

courbe) - [P242] : 415 -> 200 (Longueur d'onde

de travail)

- [P028] : 5 -> 0 (Temps d'application

de la H.T. à l'init) - [P102] : 0 -> 10 (Temps de pré-

rinçage Rec1 avec tampon)

- [P182] : 1 -> 2 (Fréquence Changement

tampon Réc2 en Lecture) - [P188] : 0 -> 1 (Nombre de cycles de

remplissage Rec1 lors du remplacement

du tampon par de l'eau) - [P259] : 1 -> 4 (Fréquence sol.

lavage en cadence rapide)

- [P457] : 20 -> 0 (Temps lavage H2O

après remplacement tampon Hémoglobine

par H2O)

- [P469] : 0 -> 2 (Validation du

remplacement du tampon de REc 1 par de

l'eau en cas d'absencede portoirs) - [P029] : 800 -> 1727 (Volume de

dilution P35+P39+P106) - [P034] : 12 -> 25 (Pas de M5 pour

expulser la dilution du fond de la

33


cupule) - [P037] : 60 -> 120 (Temps d'attente

avant remontée de l'aiguille après

dépôt fond cupule)

- [P111] : 15 -> 40 (Nombre de pas M5

pour dégagement cellule) - [P118] : 2740 -> 0 (Nombre de pas M3

pour Volume de solution hémolysante

prélevé pour la dilution hémoglobine) - [P119] : 150 -> 0 (Nombre de pas M3

pour bulle d'air avant melange après

dilution) - [P170] : 1 -> 0 (Type de réactif

utilisé pour les lavages et dilutions) - [P174] : 4 -> 1 (Nb Rempl/Vid Mini-

puit cupule mélange I.T) - [P228] : 26 -> 0 (Descente ascenseur

pour prelevement nouvelle cupule IT)

- [P281] : 37 -> 0 (Plongée après

detection pour diluant en pas M5) - [P283] : 200 -> 0 (Pas M5 descente

decontamin. Ext. Aiguille Hb/Urine)

- [P284] : 150 -> 0 (Pas M3 goutte dé

decontamin. Ext. aiguille HB/Urine) - [P285] : 1 -> 0 (Validation rinçage

aiguille entre les dilutions) - [P286] : 1 -> 0 (Validation du

mélange après chaque dilution) - [P287] : 2 -> 0 (Nb lavage externe

l'aiguille en HB/Urine) - [P288] : 30 -> 0 (Nombre de pas M3

pour volume non rééjecté à la fin d'un

mélange) - [P608] : 127 -> 255 (Nombre et choix

des code-barres tubes à lire) - [P094] : 240 -> 81 (Acquisition

delay) - [P001] : 340 -> 355 (Starting up

Temperature)

- [P002] : 340 -> 355 (Reading

Temperature) - [P003] : 400 -> 355 (Activation

Temperature)

- [P015] : 8 -> 20 (Injection vacuum) - [P016] : 6 -> 1 (Injection time) - [P020] : 9800 -> 7800 (Migration

voltage)

- [P086] : 495 -> 238 (High voltage

migr. time)

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- [P093] : 20 -> 50 (Current minimum) - [P123] : 340 -> 355 (Ready state

Temperature) - [P126] : 340 -> 355 (Shutdown

Temperature) - [P039] : 800 -> 1482 (Steps M3 for

volume in Dilution segment cup)

- [P081] : 21 -> 9 (Pas codeur M5 pour

pénétration dans sérum) - [P096] : 6 -> 5 (Dilution ratio

200µl)

- [P138] : 90 -> 20 (Volume mélangée

dans cupule simple en IT)




info 01/26/07 09:55:48

Phase MP_Initialise: OUT level = 52500 µl (5%)

H2O level = 462000 µl (66%) NaOH level = 259000 µl (37%) BUFFER level = 469000 µl (67%)




error 01/26/07 09:51:55

STOP IN COM ERROR AFTER MAX RETRY :

User stopped the connexion with

Capillarys.




info 01/26/07 09:15:42

Phase MP_Barcode: OUT level = 51151 µl (4%)



info 01/26/07 09:15:06 Exiting MP_Start state ...

info 01/26/07 09:15:03 Entering MP_Start state ...


info 01/26/07 09:11:39 ** Ready **


0:00:09)





info 01/26/07 08:51:41 Automatic level updating : (phase

35


MP_WaitInitialise) H2O level = 497000 (71%)



info 01/26/07 08:49:56

Phase MP_Initialise: OUT level = 273000 µl (26%)





Exemple of error message displayed in red

error 06/20/05 14:44:09

DISTRIBUTOR - Tank 2 filling time out

with the buffer solution: check the

buffer container level or replace

filter.

11) Firmware update

New dialogue window for the 3 additional cards + the DCN card:

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Tank card Container card Rack introduction card

The new cards are loaded after the 3 main cards (CCD, Passeur/Sampler and Distributor).

Order and required time to update the boot and firmware on the versions 5.29 or 5.40 with the new microcontrollers 89C669 in place on the CCD, Passeur/Sampler and Distributor cards: CCD card 7‟, Passeur/Sampler card 7‟, Distributor card 7‟, DCN + Cuve/Tank + Bidon/Container + Portoir/Rack introduction cards + parameter upload. 3‟ : total 25‟ approx. It takes 15‟ on the versions 5.21 (and previous ones) with the microcontrollers 89C664 (µC with 64KB instead of 96 KB of memory).

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12) Cycle timing on Capillarys 1 & Capillarys 2 (rel. 6.xx)

CYCLE CAPILLARYS 1 CAPILLARYS 2

Normal Startup 20 min 20 min

HR Startup 25 min 25 min

Normal shutdown (Protein 6, IT) 13 min 13 min

HR & Hb shutdown 21 min 21 min

Restart after a warning error (8xxx error) 8 min 1 min

Restart after a major error (0xxx error) 8 min 8 min

Capillary activation 28 min 28 min

Capiclean cycle (capillaries + sample probe) 30 min 45 min

Sample probe cleaning (Hypochloride or CDT wash solution)

6 min 6 min

Technique change (PROTEIN 6 or B1-B2+ ↔ CDT) 45 min 30 min

Technique change (PROTEIN 6 or B1-B2+ ↔ HR) 45 min 30 min

Technique change (PROTEIN 6 or B1-B2+ ↔ IT) 7 to 9 min 1 min

Technique change (PROTEIN 5 → Any techniques) 45 min 30 min

Technique change (Any techniques → PROTEIN 5) ~ 2H 30 min

Test solution #2 (change from Protein 5, 6 or B1-B2+)

8 min 8 min

Test solution #2 (analysis cycle) 13 min 13 min

Test solution #2 (Return to PROTEIN 5, 6 or B1-B2+)

8 min 8 min

Note: times measured with new reagent filters in place. 13) Precautions when dismantling the system

Refer to the Capillarys 2 technical CD-ROM (Pictures\Instrument dismantling)

Precautions when dismantling something on the fluidics Air valves do not over tight the 2 screws on the manifold and pay attention to the square seal and the position of the valve because some of them are mounted upside down (see schematic).

Liquid valves do not over tight the 2 screws and pay attention to the 2 little O-rings and the position of the valve because some of them are mounted upside down.

4-way valves do not over tight the thin tubing on the 4-way valve, the syringe and the cathodic tank. More tight it is and smaller is the diameter in the tube (tube too constricted or shrunk) to let the liquid pass through the fitting. When it is the case, filling errors on the tank 2, bad dilutions and also bad injection can occur.

Manifold do not over tight the pressure quick couplings for the blue and pink tubing on the manifold and the screws used to fix the air valves.

Pressure safety valve the pressure reservoir is purged with an over pressure above 3.5 bars to open the safety valve during few seconds when starting up and

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shutting down the system and then let escape the water condensation. So if the system does not "whistle" for few seconds during the startup and shutdown, the ball with the spring on the safety valve could be blocked. It can happen when the system has not been used for a while. It can be unblocked pretty easily from the opening in the chassis underneath the pressure reservoir by hitting the safety valve with a screw driver or passing something through the output aperture.

Precautions when dismantling the conveyor on the units SN 401 to 700

Be careful when dismantling the conveyor on Capillarys 2 because it is pretty easy to break the opto sensor (capt.22) of the motor 11 (motor used to stop the sample rack for the dilution segment detection) as shown on hereafter on the pictures.

A new bracket is in place since the SN 701 to prevent such kind of mistake. Note that a spare opto (which is not used) is available on the 4 way-valve EV 19 for the additional buffers!

14) Recommended spare parts

Quantity

Description

Capillarys 1 compatibility

Part number

1 Maintenance Kit Yes P/N 10046710

1 Deuterium Lamp Yes P/N 10046252

10 Capillary Cartridge Yes P/N 10046286

1 CCD PCB Yes P/N 10051283

1 Front Electrode Assembly (anode) No P/N 10051289

1 Sampler/Passeur PCB Yes P/N 10051290

1 Distributor PCB* See note P/N 10051291

1 Valve PCB No P/N 10051292

1 Syringe Ceramic Piston Yes P/N 10045334

2 Test Solution No.2 (10 ml) Yes P/N 38000004

1 Teflon 4-Way Valve #16-18-19 No P/N 10051902

1 Syringe O-ring Yes P/N 40000262

5 Reagent Filter Yes P/N 40000410

1 Air Filter Yes P/N 40000411

4 Air Valve #1-2-3-5-7 No P/N 45360033

2 Liquid Valve #11-12-13-15-17-21 No P/N 45360034

2 Pump Driver IFR 540 Yes P/N 45732004

1 Sensor #14 No P/N 10051C14

2 Sensor #10-16-17-19-21 No P/N 10051C10

1 Sensor #07-08-09-15-18-22-27 No P/N 10051C07

1 Sensor #29 No P/N 10051C29

1 Sample probe Yes P/N 10051217

1 Set of Microcontrolers (ACEX) No P/N 495014-3

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(*) Distributor PCB: for the new temperature probe on Capillarys 2, the resistors R2 and R3

have been removed (10 K previously) and R11 is equal to 0 (instead of 56.2 K on Capillarys 1). It means that the temperature probe has to be replaced by the new one on Capillarys 1 to use the same board.

15) Capillarys principle (reminder) Capillary electrophoresis has been used in research for many types of separations (single capillary). Recent advances in technology have allowed the methodology to more easily be adapted for routine clinical use: - Multi-channel - Fast separations - Positive sample ID - Complete automation

• The technique is based on an electrokinetic separation in a capillary filled with electrolyte buffer.

• The combination of:

small capillary internal diameter (<100 µm). Note that it is 25 µm on Capillarys, very high voltage,

tight temperature control, Insuring: fast, highly efficient separation of proteins, excellent resolution and reproducibility: sample-to-sample, cap-to-cap, run-to-run.

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41

Physical parameters influencing the analysis

• Temperature (injected volume, migration speed, protein solubility, capillary overheating, protein denaturation)

• Voltage (resolution, migration time, capillary overheating)

• Injection (resolution, sensitivity)

• Capillary (length, internal diameter)

• Wave length detection

Chemical parameters influencing the analysis

• pH (electro-osmotic flow, charge, protein solubility)

• Ionic strength (electro-osmotic flow, protein solubility)

• Buffer composition (buffer capacity, interactions, conductivity and absorbance)

• Sample diluent composition

• Additives

16) Electronics and hydraulics bloc diagrams and schematics

See next pages.

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43

44

45

46

47

48

49

50

51

52

53

17) Troubleshooting the system 17.1) Migration problems observed on the curves

The more frequent migration problems are listed hereafter:

OBSERVATION PROBABLE CAUSES CORRECTIVE ACTIONS

Baseline observed on one capilllary

No sample, cap on the sample tube or insufficient volume in the tube Clogged capillary

Check the corresponding sample tube (position 1 to 8) and volume (50 µl min.), If volume too small, transfer the sample in a microtube. Run a capillary activation cycle and run new sample-racks. If the problem still persists, replace the corresponding capillary. .

Baseline observed on all capillaries

No sample, cap on the sample tubes or insufficient volume in the tube Lamp shutter is blocked Deuterium lamp is burned out Bad dilution, dilution level too low in the dilution segment Capacitive level sensor of the sample probe faulty

Check the sample tubes and volumes (50 µl min.).), If volume too small, transfer the samples in microtubes. Check the shutter from the service menu. Replace the deuterium lamp and launch a new priming. Check the lamp power supply output. Check the buffer container (container empty, tighten the cap, replace the filter, replace the container, check the quick coupling…). Check the syringe Check the capacitive level sensor adjustment with the oscilloscope and the probe out of the washing block.

Too long migration or delayed migration observed on one capilllary

Clogged capillary

Run a new sample-rack to confirm the problem, then launch the shutdown cycle and restart Capillarys. If the error remains, replace the corresponding capillary.

Too long migrations or delayed migrations observed on all capillaries (continued)

Bad washing of the capillaries Inversion of the reagent containers Too high injection or bad dilution (too concentrated) Migration temperature regulation problem (temperature too low) Brand new capillaries

Change all the reagents (H2O, wash and buffer solution), and run new sample-racks. If the problem remains, run a capillary activation cycle and run new sample-racks. Check the position of the containers (color codes): if there is an inversion, put them back in their right position, launch a shutdown cycle and restart Capillarys. Check the reagent containers (containers empty, tighten the cap, replace the reagent filters). Check the air valves 4,5 and 21 Check the temperature (35°C) Run a capillary activation cycle.

54

OBSERVATION PROBABLE CAUSES CORRECTIVE ACTIONS

Low OD on one capillary and/or weak resolution

Insufficient volume in the sample tube Viscous serum Clogged capillary

Check the volume of sample in the corresponding primary tube: if it is too small, transfer the sample in a microtube. Run a capillary activation cycle and run new sample-racks. If the problem still persists, replace the corresponding capillary.

Low OD and/or weak resolution on all capillaries

Bad dilution, dilution level too low in the dilution segment Clogged capillaries Lamp energy too weak

Check the buffer container (container empty, tighten the cap, replace the filter…). Run a capillary activation cycle and run new sample-racks. Replace the deuterium lamp.

17.2) Capillarys status messages (rel. 5.21 & above) They inform the operator of the cycle in progress and the status of Capillarys.

0x0501=Communication port check... 0x0502=Instrument connection check... 0x0503=Firmware upload in progress... 0x0504=Parameters upload in progress... 0x0505=Start initialization 0x0506=Priming... 0x0507=Calibration... 0x0508=Priming... 0x0509=Ready. 0x050A=Shutdown command 0x050B=Shutdown in progress 0x050C=Ready for Shutdown. 0x050D=Start 0x050E=Barcode reading... 0x050F=Wait for barcode check... 0x0510=Wait for barcode answer... 0x0511=Abort 0x0512=Migration in progress... 0x0513=Read in progress... 0x0514=Background calibration... 0x0515=Activation cycle... 0x0516=Ready for reagents change 0x0517=Check reagent... 0x0518=Change reagents request 0x0519=Test mode request... 0x051A=TEST MODE 0x051B=Dilution in progress... 0x051C=Waiting for rack... 0x051D=Erasing FLASH 0x051E=Upload processor

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0x051F=Upload FPGA 0x0520=First-wash in progress... 0x0521=First-wash completed. Please restart the instrument normally. 0x0522=Wash with CAPICLEAN in progress... 0x0523=The Capillarys customized parameters (on Distributor PCB) are different from the ones saved on the PC. Do you want to keep the current instrument setting? If NO, the parameters saved on the PC will be loaded on Capillarys and will have to be checked. 0x0524=Cleaning of the sample probe in progress... 0x0525=Upload boot 0x0526=Erasing boot 0x0527=Capillarys does not have any customized parameters. For security, we highly recommend to check the customized parameters as soon as Capillarys will be in the state 'Ready'. 0x0528=The Capillarys serial number connected to your computer has changed since last connection. For security, we highly recommend to check the customized parameters as soon as Capillarys will be in 'Ready' mode. 0x0529=Your PC does not have any customized (modified) parameters to send to Capillarys. For security, we highly recommend to check the customized parameters as soon as Capillarys will be in 'Ready' mode. 0x052A=Closing of instrument connection. 0x05FE=Warning 0x05FF=Error

17.3) Error messages (rel. 5.21 & above) The error messages that can appear on the screen are listed hereafter, they are divided in 6 categories:

Errors from the serial link between Capillarys and PC,

Errors from the CCD board and optical module,

Errors from the sampler/passeur module,

Errors from the distributor module,

Errors from the rack introduction module,

Miscellaneous. When an error appears, a warning window with the error message is displayed on the screen. It ask either to wait (i.e. Inlet conveyor full!), to act (Outlet conveyor full! or Waste tank full!).

Errors from the serial link between Capillarys and PC 0x0601=Impossible to open communication port 0x0602=No answer from instrument 0x0603=Firmware upload error 0x0604=Parameters upload error 0x0605=Initialization command error 0x0606=Error during instrument status request 0x0607=Error during calibration process, restart the system. 0x0608=Error during Shutdown request 0x0609=Error during Shutdown process 0x060A=Error during Start request 0x060B=Error during Barcode reading 0x060C=Error during Barcode process 0x060D=Error during Barcode decision answer

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0x060E=Error during Abort request 0x060F=Error during migration... 0x0610=Error during reading... 0x0611=Waste tank full! 0x0612=Outlet conveyor full! 0x0613=Inlet conveyor full! 0x0614=Error during the reagent change request 0x0615=Error during the reagent validation request 0x0616=Error during the test mode request 0x0617=Error during the sensor test request 0x0618=Customized parameters upload error 0x0619=Error during the error status request 0x061A=Error during the quick stop request 0x061B=Error during the instrument serial number request 0x061C=Error during the technique change final status request 0x061D=Error during the technique change process 0x061E=Error during the Running In process 0x061F=Error during a flash memory access 0x0620=Error during an eeprom memory access 0x0621=Error during TEST MODE 0x0622=Error during reagent lighting commands 0x0623=Error during the flash, eeprom or firmware version check

Corrective actions:

Wait (i.e. Inlet conveyor full!), open the outlet conveyor lid to remove the run sample-racks (Outlet conveyor full!), drain the waste container (Waste tank full!) and click on Yes to continue. For the other errors, make sure that Capillarys is switched on, check the serial link port (COM) and cable between Capillarys and the PC, shutdown and restart Capillarys and PC.

Uploading error 0x1000=CCD board upload: no error 0x1021=CCD board FPGA upload: nStatus FPGA detected to 1 during init 0x1022=CCD board FPGA upload: nStatus FPGA detected to 0 during upload 0x1023=CCD board FPGA upload: ConfDone FPGA detected to 0 at the end of the upload 0x1024=CCD board FPGA upload: wrong writing into external RAM 0x1067=CCD board firmware upload: writing problem into flash memory 0x1065=CCD board firmware upload: wrong checksum of the firmware 0x1010=Unknown reception order during CCD board upload 0x2000=Sampler board upload: no error 0x2021=Sampler board FPGA upload: nStatus FPGA detected to 1 during init 0x2022=Sampler board FPGA upload: nStatus FPGA detected to 0 during upload 0x2023=Sampler board FPGA upload: ConfDone FPGA detected to 0 at the end of the upload 0x2024=Sampler board FPGA upload: false writing into external RAM 0x2067=Sampler board firmware upload: writing problem into flash memory 0x2065=Sampler board firmware upload: wrong checksum of the firmware 0x202e=Sampler board FPGA upload: no I2C response from the Sampler board 0x204e=Sampler board boot upload: no I2C response from the Sampler board 0x205e=Sampler board firmware upload: no I2C response from the Sampler board

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0x202f=Sampler board FPGA upload: I2C dialogue error 0x204f=Sampler board boot upload: I2C dialogue error 0x205f=Sampler board firmware upload: I2C dialogue error 0x2010=Unknown reception order during Sampler board upload 0x3000=Distributor board upload: no error 0x3021=Distributor board FPGA upload: nStatus FPGA detected to 1 during init 0x3022=Distributor board FPGA upload: nStatus FPGA detected to 0 during upload 0x3023=Distributor board FPGA upload: ConfDone FPGA detected to 0 at the end of the upload 0x3024=Distributor board FPGA upload: false writing into external RAM 0x3067=Distributor board firmware upload: writing problem into flash memory 0x3065=Distributor board firmware upload: wrong checksum of the firmware 0x302e=Distributor board FPGA upload: no I2C response from the Sampler board 0x304e=Distributor board boot upload: no I2C response from the Sampler board 0x305e=Distributor board firmware upload: no I2C response from the Sampler board 0x302f=Distributor board FPGA upload: I2C dialogue error 0x304f=Distributor board boot upload: I2C dialogue error 0x305f=Distributor board firmware upload: I2C dialogue error 0x3010= Unknown reception order during Distributor board upload 0x4000=DCN board upload: no error 0x404e=DCN board boot upload: no I2C response from the Sampler board 0x405e=DCN board firmware upload: no I2C response from the Sampler board 0x404f=DCN board boot upload: I2C dialogue error 0x405f=DCN board firmware upload: I2C dialogue error 0x5000=Tank board upload: no error 0x504e=Tank board boot upload: no I2C response from the Sampler board 0x505e=Tank board firmware upload: no I2C response from the Sampler board 0x504f=Tank board boot upload: I2C dialogue error 0x505f=Tank board firmware upload: I2C dialogue error 0x6000=Reagent container board upload: no error 0x604e=Reagent container board boot upload: no I2C response from the Sampler board 0x605e=Reagent container board firmware upload: no I2C response from the Sampler board 0x604f=Reagent container board boot upload: I2C dialogue error 0x605f=Reagent container board firmware upload: I2C dialogue error 0x7000=Rack introduction board upload: no error 0x704e=Rack introduction board boot upload: no I2C response from the Sampler board 0x705e=Rack introduction board firmware upload: no I2C response from the Sampler board 0x704f=Rack introduction board boot upload: I2C dialogue error 0x705f=Rack introduction board firmware upload: I2C dialogue error

Corrective actions:

Check the I2C bus cable into the system: it could be disconnected, scratched or pinched somewhere. Update boot and firmware on corresponding board, If it does not help, replace the microcontroller on the board (CCD, Sampler or Distributor) or replace board itself (Tank, DCN, Reagent container or Rack introduction).

Errors from the CCD board 0x0001=CCD - Fifo faulty 0x0002=CCD - Calibration impossible: Saturation. 0x0003=CCD - Calibration impossible: Peak level(s) too weak. 0x0004=CCD - Calibration impossible: Peak level(s) too high.

58

0x0005=CCD - Calibration impossible: Values out of range. 0x0006=CCD - Calibration impossible: Incoherent data. 0x0007=CCD - Emptying RAM in acquisition. 0x0008=CCD - Calibration impossible: Deuterium lamp off. 0x0009=CCD - Peak level(s) lower than minimum threshold (P157). 0x000A=CCD - Peak level(s) higher than maximum threshold (P156). 0x000B=CCD - Background level too high (>0.5 V) or lamp shutter blocked. 0x000C=CCD - Signal level higher in migration (P162 too high). 0x000D=CCD - Error connection with EEPROM I2C. 0x000E=CCD - Fifo empty in clear phase. 0x000F=CCD - Integration time lower than minimum threshold (< 10 ms). 0x0010=CCD - Gaussian area too low, no light on the CCD. Restart the system. 0x0011=CCD - Error with the management of the instrument customized parameters. 0x0012=CCD - No light: lamp off or lamp shutter blocked. 0x0013=CCD - PC/Capillarys no connection after time out (P139). 0x0014=CCD - Migration time too long according to integration time max. entered. 0x0015=CCD - CCD calibration data incoherent: re-start the system 0x0020=CCD - Entered integration time too long for calibration with the monochromator. 0x0021=CCD - No detection of the peak at 656 nm. No light, lamp shutter blocked, integration time too low… 0x0050=CCD - Serial command (RS-232) unknown. 0x0051=CCD - I2C destination address unknown. 0x0052=CCD - I2C error during the reading task. 0x0053=CCD - I2C error during the standby task. 0x0054=CCD - I2C link buffer saturated. 0x0057=CCD - Dialogue I2C impossible with the Container detection board. 0x0058=CCD - Dialogue I2C impossible with the Rack introduction board. 0x0060=CCD - No reception of I2C response from the Distributor board 0x0061=CCD - No reception of I2C response from the Sampler board 0x0062=CCD - I2C dialogue error with the Distributor board 0x0063=CCD - I2C dialogue error with the Sampler board 0x0064=CCD - No reception of I2C response from the Tank board 0x0065=CCD - I2C dialogue error with the Tank board 0x0070=CCD - No detection of the monochromator home position. 0x0071=CCD - Movement Time out from one of the Tank board motors. 0x0072=CCD - Unknown error from the Tank board. 0x0073=CCD - Tank board motors not initialized. 0x0074=CCD - One of the Tank board motors is already in progress.

Corrective actions:

Check the CCD calibration: from the main menu, select respectively Capillarys/Service/CCD calibration data, then check the Integration time, this one should be lower than 100 ms otherwise replace the deuterium lamp and launch a new starting up. Check the CCD board connection.

Errors from the SAMPLER/PASSEUR module

0x0112=SAMPLER - Barcode reader communication error. 0x0101=SAMPLER - Wash detection status not finished on Distributor board. 0x0102=SAMPLER - Carousel tray in wrong position for sample rack transfer from inlet conveyor.

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0x0111=SAMPLER - Carousel tray in wrong position for sample rack transfer to outlet conveyor. 0x0113=SAMPLER - Motor 3: Always in home position after clearing of opto sensor 14. 0x0143=SAMPLER - Motor 3: Motor blocked. 0x0153=SAMPLER - Motor 3: Syringe in full position before filling request. 0x0163=SAMPLER - Motor 3: Syringe empty before emptying request. 0x0170=SAMPLER - Leak in the waste circuit. 0x0171=SAMPLER - Introduction of a second sample rack in running in mode. 0x0173=SAMPLER - Motor 3: Too much play in the syringe (>200 steps or 27 µl). 0x0104=SAMPLER - Motor 4: Return to home position not detected. 0x0114=SAMPLER - Motor 4: Always in home position after clearing of opto sensor 15. 0x0124=SAMPLER - Motor 4: Motor blocked. 0x0134=SAMPLER - Motor 4: 4-way valve (EV18) is not in position 1 (home position) before rotation. 0x0144=SAMPLER - Motor 4: 4-way valve (EV18) is already in position 1 (home position) before rotation request to this position. 0x0105=SAMPLER - Motor 5: Return to home position not detected. 0x0115=SAMPLER - Motor 5: Always in home position after clearing of opto sensor 16. 0x0125=SAMPLER - Motor 5: Empty tube detected by the motor encoder 0x0155=SAMPLER - Motor 5: Reversed rotation movement of the sample probe motor detected 0x0165=SAMPLER - Motor 5: Empty tube detected by the end of the motor 5 movement given by the parameter P223 setting (motor steps max.) 0x0175=SAMPLER - Motor 5: Liquid detected before pick-up inside sample tube. 0x0145=SAMPLER - Motor 5: Absence of CDT diluent or hemolysing solution for the Hb (depending of the technique used). 0x0106=SAMPLER - Motor 6: Return to home position not detected. 0x0116=SAMPLER - Motor 6: Always in home position after clearing of opto sensor 17. 0x0126=SAMPLER - Motor 6: Motor blocked. 0x0137=SAMPLER - Motor 7: Motor blocked. 0x0147=SAMPLER - Motor 7: Sample rack already in up position before up movement request. 0x0157=SAMPLER - Motor 7: Problem with the dilution segment presence sensor 20. 0x0108=SAMPLER - Motor 8: Motor blocked. 0x0118=SAMPLER - Motor 8: Sample rack jam on the outlet conveyor belts in running in mode. 0x0128=SAMPLER - Unloading problem of the sample rack on the outlet conveyor detected by the sensor 28. 0x0109=SAMPLER - Motor 9: Return to home position not detected. 0x0119=SAMPLER - Motor 9: Motor blocked. 0x0129=SAMPLER - Motor 9: Reading error of the coder register. 0x0139=SAMPLER - Motor 9: Incorrect positioning. 0x0149=SAMPLER - Motor 9: Motor blocked (blockage detected by encoder M9). 0x0159=SAMPLER - Motor 9: Motor blocked. 0x0169=SAMPLER - Motor 9: Mismatch between the motor and encoder steps on the motor M9. 0x0179=SAMPLER - Motor 9: Wrong position of the sample rack before dilution. 0x01a9=SAMPLER - Motor 9: No detection of carrier movement (shift) from the home position sensor. 0x011A=SAMPLER - Motor 10: Motor blocked. 0x012A=SAMPLER - Motor 10: Incorrect positioning. 0x013A=SAMPLER - Motor 10: Sample rack not picked-up after up movement. 0x014A=SAMPLER - Motor 10: Sample rack in loading position before lateral movement of motor M9 carrier (problem with sensor 29). 0x011B=SAMPLER - Motor 11: Motor blocked.

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0x012B=SAMPLER - Motor 11: Sample rack blocker already in down position before descent request. 0x013B=SAMPLER - Motor 11: Sample rack blocker detected in low position with the opto sensor 22 after rising request. 0x011C=SAMPLER - Motor M12 blocked. 0x0135=SAMPLER - Dilution segment cup full before filling. 0x0180=SAMPLER - Air leak in the pressure circuit (tighten the reagent container caps). 0x0181=SAMPLER - Customized parameters treatment error. 0x0182=SAMPLER - Unknown I2C request. 0x0183=SAMPLER - Motor 3: Time out 0x0184=SAMPLER - Motor 4: Time out 0x0185=SAMPLER - Motor 5: Time out 0x0186=SAMPLER - Motor 6: Time out 0x0187=SAMPLER - Motor 7: Time out 0x0188=SAMPLER - Motor 8: Time out 0x0189=SAMPLER - Motor 9: Time out 0x018A=SAMPLER - Motor 10: Time out 0x018B=SAMPLER - Motor 11: Time out 0x0190=SAMPLER - No vacuum detected in the waste container by the sensor 34 after vacuum request. 0x0191=SAMPLER - Opening problem of the sample probe washing block draining valve EV17. 0x0192=SAMPLER - Sample rack detected on the inlet conveyor with the opto sensor 24 before detection of its introduction (problem with sensor 23). 0x0193=SAMPLER - Dialogue error with the I2C EEPROM. 0x0199=SAMPLER - Reading problem of the encoder/motor step registers for the motor 9. 0x0132=SAMPLER - Dialogue I2C impossible with CCD board 0x0136=SAMPLER - Dialogue I2C impossible with Distributor board 0x0154=SAMPLER - Dialogue I2C impossible with DCN board 0x0180=SAMPLER - Dialogue I2C impossible with Reagent container board 0x01C2=SAMPLER - Dialogue I2C impossible with Rack introduction board 0x0122=SAMPLER - Dialogue I2C impossible with Tank board 0x01A0=SAMPLER - Dialogue I2C impossible when writing into the bottom part of the EEPROM 0x01A1=SAMPLER - Dialogue I2C impossible when writing into the top part of the EEPROM 0x01A2=SAMPLER - Dialogue I2C impossible when reading the bottom part of the EEPROM 0x01A3=SAMPLER - Dialogue I2C impossible when reading the top part of the EEPROM 0x01A4=SAMPLER - I2C buffers full on Sampler board 0x01A5=SAMPLER - No I2C response from Distributor board

Corrective actions:

Open the sampler lid to check if there is no mistake with the involve motor: jam in line with the sample-racks on the inlet/outlet conveyor, motor or opto sensor partially disconnected. Perform the test of the corresponding motor and opto from the service menu.

Errors from the DISTRIBUTOR module 0x0201=DISTRIBUTOR - Motor 1: Return to home position not detected. 0x0202=DISTRIBUTOR - Motor 2: Return to home position not detected. 0x0203=DISTRIBUTOR - Motor 1: Motor blocked. 0x0204=DISTRIBUTOR - Motor 2: Motor blocked.

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0x0205=DISTRIBUTOR - Carousel tray in wrong position for phase 1 (low pos. for Filling/Injection). 0x0206=DISTRIBUTOR - Carousel tray in wrong position for phase 2-4 (high pos. for Filling/Injection). 0x0207=DISTRIBUTOR - Carousel tray in wrong position for phase 3 (high pos. for Washing/Migration). 0x0208=DISTRIBUTOR - Carousel tray in wrong position for intermediate phase 2-3 or 3-1 (low pos. for Washing/Migration). 0x0209=DISTRIBUTOR - Motor 1: Time out 0x020A=DISTRIBUTOR - Motor 2: Time out 0x020B=DISTRIBUTOR - Motor 2: Problem in the FPGA registers status. 0x0210=DISTRIBUTOR - Pressure: Rising time too long. The reagent containers are not engaged properly or the caps are not well tightened. Check the containers and click on Retry. 0x0211=DISTRIBUTOR - Pressure: Measurement error (High threshold reached before pressure regulation). 0x0212=DISTRIBUTOR - Pressure: Measurement error (Low pressure measurement reached immediately after application of the high threshold). 0x0213=DISTRIBUTOR - Pressure: Threshold value higher than the sensor accepted range. 0x0214=DISTRIBUTOR - Vacuum time out for injection. 0x0215=DISTRIBUTOR - Vacuum high threshold reached before applying vacuum (sensor 2). 0x0216=DISTRIBUTOR - Vacuum threshold value higher than the sensor accepted range. 0x0217=DISTRIBUTOR - Vacuum sensor offset higher than 40 mBars. 0x0218=DISTRIBUTOR - Vacuum sensor error. 0x0219=DISTRIBUTOR - Pressure sensor offset higher than 400 mBars. 0x021A=DISTRIBUTOR - Detection error from the pressure sensor. 0x021B=DISTRIBUTOR - Air emptying time out (EV7-EV21). 0x021C=DISTRIBUTOR - Overpressure time out in the tank 2 (cathode). 0x021D=DISTRIBUTOR - No release of the safety valve at the bottom of the pressure reservoir. 0x0220=DISTRIBUTOR - Tank 1 draining time out. 0x0221=DISTRIBUTOR - Tank 2 draining time out. 0x0222=DISTRIBUTOR - No liquid detected by sensor 5 after time out (P84) at the beginning of the tank 1 draining. 0x0223=DISTRIBUTOR - No liquid detected by sensor 5 after time out (P19) at the beginning of the tank 2 draining. 0x0225=DISTRIBUTOR - Opening problem of EV7 when injecting the pressure in the tank 2. 0x0230=DISTRIBUTOR - Tank 1 filling time out with the water: check the water container level or replace filter. 0x0231=DISTRIBUTOR - Tank 2 filling time out with the water: check the water container level or replace filter. 0x0232=DISTRIBUTOR - Liquid detected in tank 1 before filling (sensor 3 activated). 0x0233=DISTRIBUTOR - Liquid detected in tank 2 before filling (sensor 4 activated). 0x0234=DISTRIBUTOR - Tank 1 filling time out with the wash solution: check the wash container level or replace filter. 0x0235=DISTRIBUTOR - Tank 2 filling time out with the wash solution: check the wash container level or replace filter. 0x0236=DISTRIBUTOR - Tank 1 filling time out with the buffer solution: check the buffer container level or replace filter. 0x0237=DISTRIBUTOR - Tank 2 filling time out with the buffer solution: check the buffer container level or replace filter. 0x0238=DISTRIBUTOR - Tank 2 fill level too high. 0x0239=DISTRIBUTOR - Tank 1 full detected by the sensor 3 after draining request of the tank.

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0x023A=DISTRIBUTOR - Tank 2 full detected by the sensor 4 after draining request of the tank. 0x0240=DISTRIBUTOR - Analog to digital converter busy before reading. 0x0241=DISTRIBUTOR - Analog to digital conversion time too long. 0x0250=DISTRIBUTOR - Output min. of the high voltage power supply incorrect at the beginning of the migration. 0x0251=DISTRIBUTOR - High voltage power supply output incorrect during the rising phase by steps. 0x0252=DISTRIBUTOR - High voltage power supply output incorrect during the migration phase. 0x0253=DISTRIBUTOR - High voltage power supply current too high at the beginning of the migration. 0x0254=DISTRIBUTOR - High voltage power supply current too high during the rising phase by steps. 0x0255=DISTRIBUTOR - High voltage power supply current too high during the migration phase. 0x0256=DISTRIBUTOR - High voltage power supply current = 0. 0x0257=DISTRIBUTOR - High voltage current too low (lower than P93 setting). 0x0258=DISTRIBUTOR – Time out for shutting down high voltage power supply. 0x0260=DISTRIBUTOR - Lamp faulty. 0x0261=DISTRIBUTOR - Temperature impossible to reach (Threshold too high). 0x0262=DISTRIBUTOR - Temperature impossible to reach (Threshold too low). 0x0263=DISTRIBUTOR - No response from the I2C Peltier power supply. 0x0264=DISTRIBUTOR - Peltier power supply current = 0. 0x0265=DISTRIBUTOR - Peltier fan(s) faulty. 0x0266=DISTRIBUTOR - Temperature regulation time out on the Peltier. 0x0267=DISTRIBUTOR - Gap between 2 temperature measurements too high. 0x0268=DISTRIBUTOR - Carousel tray in wrong position for the injection phase after I2C request. 0x0269=DISTRIBUTOR - Carousel tray always in injection phase position after I2C withdrawal request. 0x0270=DISTRIBUTOR - Air leak in the vacuum circuit of the waste container (tighten the container cap). 0x0271=DISTRIBUTOR - Air leak in the pressure circuit of the reagent containers (tighten the container caps). 0x0273=DISTRIBUTOR - Error pump (time out). 0x0274=DISTRIBUTOR - Dialogue error with the I2C EEPROM. 0x0275=DISTRIBUTOR - Saturation of I2C Buffer 0x0276=DISTRIBUTOR - I2C Connection error with SAMPLER board. 0x0277=DISTRIBUTOR - The sampler does not send the end of the CAPICLEAN cycle. 0x0278=DISTRIBUTOR - Error in the instrument customized parameters. 0x0280=DISTRIBUTOR - Unknown I2C link request. 0x0281=DISTRIBUTOR - Tank board initialization incorrect. 0x02a0=DISTRIBUTOR - Dialogue I2C impossible with CCD board 0x02a1=DISTRIBUTOR - Dialogue I2C impossible with Sampler board 0x02a2=DISTRIBUTOR - Dialogue I2C impossible with Tank board 0x02a3=DISTRIBUTOR - Dialogue I2C impossible with Rack introduction board 0x02a4=DISTRIBUTOR - Dialogue I2C impossible with Reagent container board 0x02a5=DISTRIBUTOR - Dialogue I2C impossible with electronic converter 0x02a6=DISTRIBUTOR - Dialogue I2C impossible with Peltier power supply 0x02a7=DISTRIBUTOR - Dialogue I2C impossible when writing into the bottom part of the EEPROM 0x02a8=DISTRIBUTOR - Dialogue I2C impossible when writing into the top part of the EEPROM

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0x02a9=DISTRIBUTOR - Dialogue I2C impossible when reading the bottom part of the EEPROM 0x02aa=DISTRIBUTOR - Dialogue I2C impossible when reading the top part of the EEPROM 0x8282=DISTRIBUTOR - No detection of the reagent 4-way valve home position (EV16 connected to the tank 2). 0x8283=DISTRIBUTOR - Motor movement impossible on the Tank board, another motor being already in progress. 0x0284=DISTRIBUTOR - Tank board motor not connected during a motor movement request(motor or 4-way valve EV16). 0x8285=DISTRIBUTOR - Motor movement impossible on the tank board during a motor movement request(motor or 4-way valve EV16). 0x8286=DISTRIBUTOR - Time out to stop the Tank board motor on the home position sensor. 0x8287=DISTRIBUTOR - Time out motor step movement on the Tank board. 0x0288=DISTRIBUTOR - Unknown error from the tank board. 0x028A=DISTRIBUTOR - Filling time out of one of the reagents in the tank 2 (cathode). 0x028B=DISTRIBUTOR - Clogged reagent filter detected when filling the tank 2 (cathode). 0x0290=DISTRIBUTOR - Wrong reception of the I2C link response. 0x0291=DISTRIBUTOR - Position error of the reagent 4-way valve for the tank 2 (cathode). 0x0292=DISTRIBUTOR - Filling time control of the tank 2 (cathode) not initialized. 0x0293=DISTRIBUTOR - Filling of the tank 2 in progress. 0x0294=DISTRIBUTOR - Response time out from the Tank board when filling the tank 2 (cathode). 0x0295=DISTRIBUTOR - Filling time of the tank 2 (cathode) incorrect. 0x0296=DISTRIBUTOR - Motor movement time out during filling. 0x0297=DISTRIBUTOR - The Reagent container board didn‟t inform the system of the pressure time out 0x0298=DISTRIBUTOR - The Reagent container board didn‟t inform the system of the vacuum time out 0x0299=DISTRIBUTOR - Measured vacuum offset too high

Errors from the CONTAINER BOARD (BIDON) 0x0709=CONTAINER BOARD - Waste container not in place.

Errors from the RACK INTRODUCTION (PORTOIR) module 0x0401=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 after clearing of the home position with the motor 16. 0x0402=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 when trying to stop the motor 16. 0x0403=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 when trying to stop the motor 16 on the home position.

Machine temporary error message 0x8117=Sample rack not placed correctly on the inlet conveyor belts. Please unlock the main cover to open it and remove the faulty sample rack. 0x8118=The main cover is open... 0x8270=Air leak, the waste container is not engaged properly or the cap is not well tighten. Check the container and click on Retry.

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0x8271=Air leak, the reagent containers are not engaged properly or the caps are not well tighten. Check the containers and click on Retry. 0x8300=CCD calibration too low: the indicated capillary position(s) on the sample rack cannot be used to run samples. Place a tube with water in the following positions:

Machine warning/info message 0x9055=I2C dialogue error between the CCD and Container board. The dialogue between the 2 boards is disabled. 0x9056=I2C dialogue error between the CCD and Rack introduction boards. Response time out. 0x9125=SAMPLER - Motor 5: Empty tube detected by the motor encoder 0x9165=SAMPLER - Motor 5: Empty tube detected by the end of the motor 5 movement given by the parameter P223 setting (motor steps max.) 0x9169=SAMPLER - Motor 9: Mismatch between the motor and encoder steps on the motor 9. 0x9192=Sample rack detected on the inlet conveyor with the opto sensor 24 before detection of its introduction (problem with sensor 23). 0x9193=SAMPLER - Sound file request error for the Tank board buzzer. 0x9145=SAMPLER - Motor 5: Absence of CDT diluent or hemolysing solution for the Hb (depending of the technique). 0x9196=Waste full... Empty the waste container to continue. 0x9195=Empty sample tube detection problem or not installed (encoder M5). When running incomplete sample racks, place a tube with water in the empty positions. 0x91a5=The sample probe height calibration with the IT segment has failed (no liquid detected or detection out of range). 0x91b5=The sample probe height has not been calibrated with the IT segment. The height will be set to the default position given by the parameter P136. 0x91c5=Register reading error of the motor 5 encoder steps. 0x9257=DISTRIBUTOR - High voltage current too low (lower than P93 setting). 0x9289=Sound file error from the Tank board. 0x9290=I2C dialogue error between the Distributor and Container board. The dialogue between the 2 boards is disabled. 0x9291=I2C dialogue error between the Distributor and Container board. Expected order -> Pressure and vacuum reading 0x9292=I2C dialogue error between the Distributor and Container board. Expected order -> Container level reading 0x9293=I2C dialogue error between the Distributor and Container board. Expected order -> Vacuum level request 0x9294=I2C dialogue error between the Distributor and Container board. Expected order -> Pressure level request 0x9295=I2C dialogue error between the Distributor and Container board. Expected order -> Unknown order 0x9242=Filling time of the tank 2 incorrect with water (> +/- 25% of the measured mean time). 0x9243=Filling time of the tank 2 with wash incorrect (> +/- 25% of the measured mean time). 0x9244=Filling time of the tank 2 with buffer incorrect (> +/- 25% of the measured mean time). 0x923B=Water container filter almost clogged. Select the button Replace containers in the instrument status window to replace the water filter after having clicked on Continue. 0x923C=Wash container filter almost clogged. Select the button Replace containers in the instrument status window to replace the wash filter after having clicked on Continue. 0x923D=Buffer container filter almost clogged. Select the button Replace containers in the instrument status window to replace the buffer filter after having clicked on Continue.

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0x9401=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 after clearing of the home position with the motor 16. 0x9402=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 when trying to stop the motor 16. 0x9403=RACK BOARD - Blockage of the rack introduction detected by the opto sensor 10 when trying to stop the motor 16 on the home position. 0x9404=RACK BOARD - The hand presence sensor 39 does not work. A new calibration of the sensor should be performed (test cycle C28). 0x9405=RACK BOARD - Motor 16 already in home position before order to return to this position 0x9701=CONTAINER BOARD - The threshold of the overflow sensor on the waste container is too low. 0x9702=CONTAINER BOARD - The threshold of the empty sensor on the waste container is too low. 0x9703=CONTAINER BOARD - The threshold of the full sensor on the water container is too low. 0x9704=CONTAINER BOARD - The threshold of the empty sensor on the water container is too low. 0x9705=CONTAINER BOARD - The threshold of the full sensor on the wash container is too low. 0x9706=CONTAINER BOARD - The threshold of the empty sensor on the wash container is too low. 0x9707=CONTAINER BOARD - The threshold of the full sensor on the buffer container is too low. 0x9708=CONTAINER BOARD - The threshold of the empty sensor on the water container is too low.

COM error 0x1100=No data received from Capillarys. 0x1101=Invalid received message from Capillarys. 0x1102=Time out on Capillarys received message. 0x1103=Checksum error on Capillarys received message. 0x1104=Too many data received from Capillarys. 0x1105=Too many NACK received from Capillarys. 0x1106=Message length received from Capillarys is different from expected one. 0x1107=Message sending error to Capillarys. 0x1108=Parity error in message received from Capillarys. 0x1109=Connection with Capillarys stopped by the operator. 0x110A=I2C problem detected. 0x110B=Capillarys buffers are full. 0x110C=Unknown error??? 0x110D=Caution: The CCD firmware file version to upload is not compatible with this Capillarys model. 0x110E=Caution: The Sampler firmware file version to upload is not compatible with this Capillarys model. 0x110F=Caution: The Distributor firmware file version to upload is not compatible with this Capillarys model.

Upload error 0x1200=Error while Uploading FPGA CCD 0x1201=Error while Uploading Boot CCD

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0x1202=Error while Uploading Bin CCD 0x1203=Error while Uploading FPGA Sampler 0x1204=Error while Uploading Boot Sampler 0x1205=Error while Uploading Bin Sampler 0x1206=Error while Uploading FPGA Distributor 0x1207=Error while Uploading Boot Distributor 0x1208=Error while Uploading Bin Distributor 0x1209=Error while Uploading Bin DCN 0x120A=Error while Uploading Boot Tank 0x120B=Error while Uploading Bin Tank 0x120C=Error while Uploading Boot Tank 0x120D=Error while Uploading Bin Tank 0x120E=Error while Uploading Boot Rack 0x120F=Error while Uploading Bin Rack

18) Installation, operator maintenance & PM check-lists

Refer to Capillarys 2 Technical CD-ROM to print the installation and maintenance log sheet document and fill them according to instructions. Same thing to print the preventive maintenance (PM) check-list document and follow the instructions.

19) Instrument calibration sheet See next 2 pages.

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-------------- Capillarys 2 Calibrations --------------

Cycle test C27 - Calibrate DCN card (Sample probe capacitive sensor)

Cycle test C28 - Calibrate rack introduction card (hand sensor)

Cycle test C29 - Calibrate container card (Reagent compartment detection levels) Fill the 4 containers, close the covers and follow the instructions.

DCN Calibration values Gain Gain PGA usually 4 Dephasage

Threshold within 400 and 4000 Offset within 800 and 8000 Coefficient

Note: The container board is used to warn the operator when a container has to be replaced. In that case the container is red. It is used also to correct the calculated volume by the system with the mid sensor on the 43containers. The calibration is saved on the Container board. The threshold values are displayed on the Distributeur-Passeur test window to be compared with the values corresponding to the current level inside the containers.

The cycles C17 and C18 are not implemented yet. The cycles C24 and C25 are not applicable on Capillarys 2 and the cycle C20 is the same as before, it has just been renamed as Capillary clogged test with pressure. The cycle C26 is used only in the

factory.

The DCN calibration is saved on the DCN board. Important: Make a Capiclean cycle with the sample rack #100 before performing this calibration. Same thing as well with a brand new needle in place.

a) Place an empty sample tube of 5 ml in position 1 on a sample rack with an Eppendhorf tube filled with 100µl of H2O at the top of the tube as shown on the picture. b) Select Cycle 27 - Calibrate DCN card from the service menu when the system is Ready (immediately after priming). Start the cycle and insert the sample rack, c) When the sample rack is introduced, click on Sensor calibration. The calibration taking approximately 1 min. d) When it is finished, the system displays the calibration values (see hereafter the accepted range for the Gain PGA, the Threshold and the Offset), e) Check the values and click on Save parameters if there are within the range, If the values are not correct, make a new calibration. If after 5 trials, there are still outside of the range, check the tube, clean up the sample probe extremity, and check the sample probe connections, the good insulation between the chassis and syringe assembly, the vertical movement of the probe, the washing block…

It is fully automatic, there is nothing to do. Avoid putting your hand or your body close to the conveyor cover to prevent any false calibration. It consists of calibrating the hand presence sensor (capacitive sensor with the metal plate) located on the cover.

Note: The expected value (threshold) is between 4000 and 8000 and is saved on the Rack introduction board. In case of

wrong calibration, the sample rack won‟t move on the inlet conveyor to be processed. The purpose of the calibration consisting to prevent any jam on the inlet conveyor by blocking the rotation of the belts until the hand of the operator has been removed from the loading entrance of the compartment.

Warning: Don’t forget to drain the waste bottle when the

calibration has been performed. been performed.

Test Cycles Mechanical settings

CCD Optical Settings

Dialogue windows used to control or calibrate the 3 positions of the motor 9 (sample rack holder) after

any shipment or dismantling of the

system.

Dialogue window used to check the optics, align the reception fiber optics on the CCD

and calibrate the Monochromator. CCD calibration data

Eppendhorf tube filled with 100µl of H2O

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Monochromator calibration (motor 14 rotation calibration at 200 and 415 nm)

Enter 12ms [a], Click on Open [b] and check the box RESPONSE CURVE [c], Click on START scan [d] to get the response spectrum of the optics (Deuterium lamp + monochromator + fiber optics + CCD).

Calibrations

CALIBRATION FROM SAVED ON

Monochromator CCD Optical setting / Response curve Distributor P.C.B

DCN (Sample probe capacitive detection)

Test Cycle C27 DCN P.C.B

Rack Introduction (Hand Sensor) Test Cycle C28 Rack Introduction (portoir) P.C.B

Reagent container Test Cycle C29 Reagent container (bidon) P.C.B

Motor 6 (P69) Forward/backward movement of the needle.

Customize the parameter from the Ready mode and Capillarys / configuration / system configuration / Technical parameters /machine customized param.. To center the needle in the dilution segment cups: (1mm corresponding to approximately 10 motor steps).

Distributor P.C.B on Capillarys and into Machine customized parameters, (modified parameters.ini) on PC

Motor 9 (P49, P51, P52) Left/right movement of the sample rack bracket

Mechanical settings Check the 3 positions of the motor 9 (sample rack holder) after any shipment or dismantling of the system.


Motor 11 (P43) Blockage of the sample rack on inlet conveyor

Customize the parameter P43 from the Ready mode and Capillarys / configuration / system configuration / Technical parameters /machine customized param.. , to have a good blockage of the sample rack on the inlet conveyor in order to detect the dilution segment with the light barrier: (1mm corresponding to approximately 10 motor steps).


Motor 5 Calibration of the system to sense the liquid with the needle extremity to determine the height of the liquid

Use the sample rack #100 and an IT segment in place with 380 µl of water in the first cup (double cup). Select the option Height calibration of the needle


Barcode reader alignment (P50) Left/right movement of the sample rack bracket (Motor 9) calibration

Customize the parameter P50 from the Ready mode and Capillarys / configuration / system configuration / Technical parameters /machine customized param..In case of bad reading of the sample rack barcode (Sample rack rejected).


Volume in the tank 1 Vertical adjustment of the (sensor 3) to have 5 ml into the tank 1.

Fill the tank 1 with H20 from the test cycle C08 and measure the volume with a plastic syringe.

Mechanical adjustment

Home position of the sample probe washing block

Adjust the sample probe extremity in the middle of the wash station with the help of the 2 little Allen screws on the top of the sample probe. After any adjustment, perform a new calibration of the motor 5 as indicated above: Use the sample rack #100 and an IT segment in place with 380 µl of water in the first cup (double cup). Select the option Height calibration of the needle.

Mechanical adjustment

The system will display successively the number of steps of the monochromator motor (motor 14) and the wavelength in nm. An error in nm is displayed for the 3 typical wavelengths (0, 200 and 415 nm) according to the theoretical curve of the monochromator. The 0 nm (zero) being the middle of the saturated peak on the right of the spectrum curve when the grating position is like a mirror returning all the wavelengths of the lamp. From the 3 errors, a correction (calibration) of the monochromator motor steps is calculated and saved on the EEPROM of the Distributor board. Note that this calibration is not visible in the customized parameters (modified parameters.ini file). Note: The response curve of the optics which is not the calibration of the monochromator can be stored on the PC (into C:\Program file\Phoresis\Capillarys\Log folder) with the Save button. A saved curve can be displayed as well with the Load button.

WARNING: Never touch or clean the diffraction grating. Even if it is a little bit dusty, it won’t have any

effect on the results.

a

b

c

d

January 2007

* * Set the integration time to have enough signal

and prevent too much saturation. The amplitude of the small peak at 656 nm should be around

0.2 to 0.3 V to have a good detection of the peak (not a spike) and then a good calibration of the

monochromator motor. Starting the rotation from the home position at approximately 700nm

when starting the scan, it is the first peak detected by the CCD.

Documents

Capillarys 2 Technical Manual