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Buffy Coat Method
Platelet Poor Plasma
Step 1: Whole Blood is collected into a top and bottom pack and is centrifuged (hard spin) to produce platelet poor plasma, red blood cells and a buffy coat
Buffy Coat Layer
Red Cell Concentrate
Red Cell Additive
Images courtesy of Canadian Blood Services – Hospital Education Presentation 2004
Step 2Extraction
Plasma
Red blood cell
Buffy Coat
Buffy Coat Method cont.
Step 3: Pooled Buffy Coats are first pooled, then sterile docked to the platelet storage bags, followed by centrifugation at low speed
Buffy Coat Method cont.
Buffy Coats steriledocked together
Plasma
Pooling (Train Method)
Buffy Coat Method cont.
Platelet storage bag sterile docked to the Buff Coat “train”
• Buffy Coat Pool is loaded into buffy coat bag
• Supernatant (PRP) pressed through LR filter into storage bag
Buffy Coat Method cont
• Pooled Platelet Concentrate, LR is Similar in physical size & dosage to an apheresis platelet concentrate
Buffy Coat Method cont.