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BRUCELLOSIS
byHASUTJI ENDAH NARUMI
DEPARTMENT OF MICROBIOLOGY; LAB OF BACTERIOLOGYFACULTY OF VETERINARY MEDICINE
AIRLANGGA UNIVERSITY
Brucellosis = Abortus Infections Disease
PRIMER attack to cow, sheep and pig
SECUNDAIR attack to other animalsand human being
COW Bang’s disease
HUMAN undulans fever (malta fever)
» Abortus» Give birth to meal» Reproduction disturbance
infertility» Milk product x» Low mortality» Treatment value» Zoonosis (important)
ECONOMIC COST
◙ - Stick- Gram (-)- Non motile- Non spora
◙ Intra seluler◙ Three species which attack to livestock
- B. militensis- B. abortus- B. suis
ETIOLOGY
Susceptible animal
EPIZOOTIOLOGY
» Cow and dog B. abortus infections abortus
» B. abortus attack horses, pigs
» B. suis pigs, dogs
» B. melitensis sheeps
Disseminating cow milk in JavaRescue institute of animal disease Bekasi, Bogor, Kediri & Jakarta Health office human which work at Denpasar
The transmission of disease
Through
Gastrointestinal tract Genitalia tract Skin, mucosa
PATHOGENESIS
Clinical symptom» Infectious abortus temporary / permanent of 5 -
8 months» Occur revers to normal» milk product» Health produce infectious vaginal liquor» Mindness of foetal liquor» Pituitary gland ≠ symptom, other side milk
contaminated againts bacteri nipple zoonosis» Epidydimitis (Male Epidydimitis) & orchitis
SYMPTOM OF THE DISEASE
‡ Thickening of placenta‡ Spreads over the chorions layers‡ Mindness of foetal of liquor, brown -
yellow, mixed with pus
2. POST MORTEM
Removal and Materials Delivery Put into ice (suggestion) Delivery to the lab Put on preserved solution
» Materials :- Milk : all nipple / not all nipples contaminated
with bacteria- Vol 20 ml < washing with alc sol 70%- 1st, 2nd out pouring should be throw among - In the cold condition
Male : Testis Inppurate Necrose
Using wire, with cotton point (absorb a thread) put the cotton in to the glass jar
Blood cultivate (from V. Jugularis)
- This area should be wash and rubbed this area using cotton talk 70%.
- Put 10 ml with spuit + heparin (anti coagulant)
Blood serologic test
Vagina : removal on 6 weeks
post partum / abortus
Placenta : infections cotyledone smears slide fictations using methanol sol
Fetus abortus :- Disposal of gatric- Pulmo- Lien- Fetal disposal- Take with sterile pasteur pipet
Carcass :- The predict of bact is different among the species
animals- Cow lgl supramamaria, lien, nipple, uterus
- Material samples- Base on :
» Clinical symptom» PA» Laboratory :
- Isolated and bacteria identification- Serologic test, blood serum thrid of mucosa vagina / milk, sperm
DIAGNOSIS
Abortus : infection camphylobacterfoetus and trichomonas foctos
C. Foetus abortus usually happenevery time
T. Foetus abortus happen earhide Brucella abortus happen after
6 months
DIFFRENTIAL DIAGNOSA
Route of administration :» to classified among
» report the sample
» to give the certificate for free Brucellosis
» to give a tag for each sample post vacc & reactor
PREVENTIVE OF THE DISEASE
Sanitation :Sanitation :» To burn the infectious material» To avoid a matting which suffer from a disease» Avoid for young animals from mother which
suffer from a disease to give milk» Crages should be disinfected to avoid suffer
from a disease» To refreshment a animals should be free from
brucellosis» To monitor the mobilitation or animals
» Standardization Dx / for Brucell (methods and reagent)
» To point out infect and healthy area from Brucellosis
» To point out infections cattles and healthy area from brucella
» To point a policy for use a vaccination» To give a certificate of health for a healthy care
ERADICATION
» Attacks animals and human
» Cause of spirochaeta bact, genus of leptospira
» The change subclinic of infectious were : abortion, hepatitis and nephritis death
Leptospirosis = Weil’s disease
F o r m : Long filament Gram (-) Aquatic saprofite apparently
ETHIOLOGY
» Resistant inside the water (1 month)» Inside the sea water, urine + rapidly
Incident in Indonesia
EPIZOOTIOLOGI
Isolation from human, dog, pig and micer / rat Serolog reactor + cow, buffalo and pig Pig at RPH Bogor, which contain bacteria
inside the ren At middle & East Java economic cost
at the cattle of pig and cow
◙ Leptospira canicola dog ◙ Leptospira harjo cow◙ Leptospira ictero haemorrhagie mice / rat◙ Human infection through water, soil, flora which
contaminated of animal urine◙ Incubation periods 4 - 19 days
The Familiar Of Several Leptospira
» Bact body through mucosal layer multiple especially in : liver, ren, mammary gland, brain layer
» Leptospiremia 1st week post infect» Bact produce toxin and hemolysin
blood capilary disturbance tissue disturbances
» Bact grow at tubulus renalis release via urine» Bact still alive in the urine several hour at 150C (several
days at 40C)» Lethal because of septichemia, anemia, liver disorder and
uremia» State of infect depend on : age, species, bact reservoir
cause of infect.
PATOGENESIS
The Route of Disease Mechanisms
Bact inside the ren come out from urine
Cow (acute, subacute and chronic)Acute : » fever, anemia, T after 2 - 4 days
» Milk prod become normal after 2 weeks » Milk produce mixed with pus
Pig : » abortus» retentio secundinarum» + neonatal» bact vehicle for cow & human
Human Infected From Contaiminated Animals (Rats)
CLINICAL SYMPTOM
Cow : liver abses, ren coloredblack dark
Calf : icterus, liver absces, dark ren, petechial haemorrhagie,
VU contain clean red urine Chronic :absces ren, if slices contained
of liquid
POSTMORTEM DISORDER
Live animal under fever condition :- Urin without preserve- Aseptic blood + citrate- Aseptic milk and not preservative
From tetal animal- Urine, liver slices, ren, lien and blood cor
From healthy animals- Urine and serum
DIAGNOSIS
Material of Sample
Bacterial Isolation Inject animal
DIFFERENSIAL DIAGNOSIS
» Babesiosis with blood strike not effective againt of leptospirosis
» Because the symtomp is abortus until confused with the cause of the other abortus
» Clinical symptom, Hb uria, cause of clostridum, haemolyticum
Death of animal - which bring the leptospira especially rodentia mild
R/ Antibiotics (Penicilline) L. canicola- Streptomycine, Aureomycine
PREVENTION