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Serving society through science
Padappai – 601 301, Kancheepuram District,
Tamil Nadu, India
INTRODUCTION IIBAT was created with the following objectives;
To serve as a link between Industry and Regulatory System in providing GLP based non-clinical health and environmental safety data on crop care/protection actives/formulations, biocides, chemicals for control of household nuisance pests and for control of pests of public health importance, industrial chemicals, herbal/phyto molecules, plant growth regulators, drugs and pharmaceuticals meant for use in clinical and veterinary medicine, transgenic crop/food, r-DNA, biopharmaceuticals and biotech based products, to facilitate risk assessment. Recent initiatives are focused on toxicity and hazard identification of engineered nano materials of industrial and therapeutic application.
To undertake basic research in above areas, in collaboration with national and international scientific community.
To train manpower, including providing opportunities for Doctoral studies, under the affiliation of local universities.
To provide employment to local communities.
To function with no profit motive.
Legal Status IIBAT is a non-commercial Research Institute. It is not controlled by
any commercial company, nor there are any share holders. IIBAT is
exempted from tax payment by the Tax authority, in view of non-
commercial operations. Further, donations to IIBAT attract tax rebates
u/s 35(i)(ii) of Tax Act 1961. IIBAT holds FCNR certificate issued by
Finance Ministry to receive donations from overseas.
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RESEARCH FACILITIES The Institution is ensconced in an area of 65 acres. The laboratory
facilities are established in a modern building GLP compliance in
more than 100,000 sq.ft., for carrying out various research studies.
The animals employed in the study are husbanded as per the
regulations of the Ministry of Forest and Environment,
Government of India (No.31/1999/CPCSEA) under Breeding and
Experiments on Animals (Control and Supervision) Rules, 1998.
Current research studies are aimed at drawing physico chemical
characterization, impurity analysis, residue, dissipation,
degradation, e-fate, stability, short and long term mammalian
toxicity, genotoxicity, reproductive and developmental toxicity,
chronic toxicity and oncogenicity/carcinogenicity, and ecotoxicity
of chemicals.
GLP Features
OECD principles of GLP are followed for the conduct of various
studies. The Institution employs highly competent scientific personnel,
proficient in basic sciences, agriculture, chemistry, genetics,
biochemistry, pharmacology, pathology, veterinary sciences and
paramedical faculties, with hands-on experience in their respective
faculty, most of them being Post Graduates and Doctorates. Studies are
supervised by qualified Study Directors and other Study Personnel for
the proper conduct of studies as per the OECD/EU principles of GLP.
All SOPs are meticulously prepared and each Study plan is
scrupulously designed and executed as per the required guidelines such
as OECD, USEPA, USFDA, EU, FAO/CIPAC, AOAC, SANCO,
RCGM-DBT-India, CIB-RC-India, DCG-India, and WHO and
others.
IIBAT is certified for GLP compliance by German GLP Authority-BfR
(until March 2011 and Indian GLP Authority of DST (since 2004).
Test Substance Controller (TSC)
TSC is controlled by LMS through LAN
system with high security for receipt,
coding, product identity, dispersal, return of
TS, TS balance, disposal and archiving.
Archive
The archive is designed for long term
retention of paper records, electronic data
and specimens with complete traceability
and security as per the regulatory
requirements.
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PRODUCTS IIBAT handled the following test substances in the research;
Organic pesticides-actives and formulated Products
Biocides
Microbial Pest Controlling Agents
Phytochemicals, semiochemicals, herbal products and
essential oils and others
Stem cells of animal origin
Engineered nanoparticles/tubes etc.,
Drugs and pharma products
R-DNA vaccines
Biopharmaceuticals/stimulants
Genetically Modified Crop/feed/proteins
TEST GUIDELINES IIBAT scientists routinely employ the following guidelines in their research;
OECD US-EPA/OPPTS/SANCO EU US-FDA ICH MAFF-UK Chinese Guidelines DCGI, Schedule Y, CIB-RC, RCGM/DBT
CURRENT SERVICES The following studies are undertaken; DEPARTMENT OF TOXICOLOGY
Six pack acute tox, short-term, sub-acute and chronic toxicity in rodents
Carcinogenicity/Teratogenicity/Developmental and Reproduction Toxicity - Generation studies
Acute, delayed and developmental Neurotoxicity
Inhalation Toxicity-head and nose exposure
Pathology (Gross pathology, Histopathology and Clinical Pathology)
LLNA – Local Lymph Node Assay
Acute tox studies are done by oral gavage, IV, SC, IM routes. Sub acute and chronic studies are done by oral gavage/dietary feeding/drinking water/head-nose exposure routes,
In vitro Toxicology
In vitro Skin
In vitro Eye
Neurotox laboratory
Responder X V 5.07
Grip Strength meter
Motor Activity cage
Open field arena
Physitemp
FOB is undertaken as a part of short-term or chronic tox studies in rodents.
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Inhalation Toxicity laboratory
Equipments imported from CH Technology, USA is available to
measure toxicity in head/nose alone exposure effect of aerosol and
dusting agents in rodents. On line analysis of particle size and
analytical dose verification is possible.
Animal Welfare
All studies utilizing animals are approved by an Institutional Animal
Ethics Committee (IAEC). Staffs are trained to handle animals with
humane care to avoid pain. Enrichment is provided.
Toxicology-Special Features
Rodents imported from Charles River laboratories, USA/UK.
Standard Gamma irradiated and extruded pellet feed to rodents
Reverse osmosis water for animals
Health monitoring of animals
Analysis of feed and water for nutrition and contaminants
Historical control data
Peer review of pathology report-all studies of 28 days and above
Enrichment
Online, continuous data capture on temperature, relative humidity in animal test rooms.
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DEPARTMENT OF ENTOMOLOGY (Crop Science)
Culturing of major agricultural crop pests viz., Helicoverpa armigera, Spodoptera litura, Plutella xylostella and Achaea janata.
Toxicity to insect natural enemies (predator & parasites) as per EPPO guideline (field study).
Phytotoxicity study of agrochemicals on various crops.
Compatibility evaluation of agrochemicals against various crop pests.
Evaluation of agrochemicals/nematicides against major crop pests under lab and field conditions.
Bioefficacy and crop injury studies of new insecticide formulations against crop pests under field/greenhouse condition.
Assessment of insect natural enemies toxicity of various agrochemicals under field condition.
Laboratory bioassay of agrochemicals, botanicals and microbial
insecticides against major crop pests viz, Helicoverpa armigera, Plutella xylostella and Spodoptera litura & estimation of LC50, potency of the same.
DEPT. OF WEED SCIENCE Evaluation of herbicides for efficacy, effect on succeeding crops,
compatibility, phytotoxicity, residue, dissipation and physico-chemical
and biological studies in soil under field conditions.
DEPT. OF AGRONOMY
Organizing field trials in IIBAT to conduct bioefficacy, compatibility, phytotoxicity, residue and dissipation studies of agrochemicals for the Department of Agronomy, Entomology, Plant Pathology and Weed science.
Evaluation of plant growth regulators/organic manures for efficacy, residue and dissipation studies under field conditions.
Maintenance of Automatic Weather Recording station..
Maintenance of medicinal plant garden and providing training to small farmers for cultivation.
Studies are undertaken in green house to evaluate seed
germination, non-target plant toxicity and vegetative vigor test as per OECD guidelines.
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DEPT. OF PLANT PATHOLOGY
Primary culture collections and maintenance of biopesticides viz., Trichoderma viride, Pseudomonas fluorescens, Beauveria bassiana, Verticillium lecanii, Metarhizium anisopliae with relevant toxicological data.
In vitro studies of biopesticides for efficacy (Biofungicides),
analytical test report, container content compatibility and shelf life.
Evaluation of fungicides for efficacy, compatibility,
phytotoxicity, residue and dissipation studies under field conditions.
Evaluation of biocontrol agents (Plant disease control) for
efficacy under field conditions.
Production of oyster mushroom (Pleurotus florida) and milky mushroom (Calocybe indica) spawn.
DEPT. ECOTOXICOLOGY
Earthworm (Eisenia fetida)
Evaluation of agrochemicals toxicity (acute and subacute) to earthworm as per OECD and ISO Guidelines
Natural enemies
Evaluation of plant protection compounds toxicity to Parasitic wasp, Aphidius rhophalosiphi and Predatory mite, Typhlodromus pyri as per IOBC, BART & ESCORT Guidelines.
Silkworm (Bombyx mori)
Evaluation of crop care compounds toxicity to silkworm assessment as per JMAFF and Chinese guidelines
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Alga (Pseudokirchneriella subcapitata) Toxicity evaluation of crop care compounds to green alga as per OECD and OPPTS Guidelines. Lemna (Lemna gibba) Toxicity evaluation of crop care compounds to Duckweed as per OECD and OPPTS Guidelines
DEPT. OF ECOTOXICOLOGY Aquatic (Danio rerio/Poecilia reticulata/
Cyprinus carpio/Oncorhynchus mykiss) – OECD Guideline
Evaluation of toxicity to fish as per OECD Guideline Daphnia (Daphnia magna) Evaluation of toxicity to daphnia as per OECD Guideline Avian: (Japanese quails, Chicken, Pigeon) OECD - Japanese quails (Coturnix conturnix japonica) CIB - Chicken (Gallus gallus domesticus) - Pigeon (Columba livia)
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DEPT. OF ECOTOXICOLOGY Honey bee (Apis cerana indica and Apis mellifera) Evaluation of oral and contact toxicity to Honey bee as per OECD Guideline. DEPT. OF ENTOMOLOGY (Household Insecticide Division)
Culturing household pests (Mosquito species viz., Culex
quinquefasciatus, Aedes aegypti and Anopheles stephensi,
Cockroach species viz., Periplaneta americana and Blattella
germanica, House fly, Musca domestica & Dust mite,
Dermatophagoides pteronyssinus).
Efficacy of household insecticides
Surface persistency of household insecticides
Efficacy and Persistency of insecticide impregnated bed net as
per WHO guideline
Efficacy and Potency of Bio larvicides like Bti
Dept. of Entomology – (Household Insecticide Division) Dept. of Entomology – ACL Facility
Approval received from RCGM to import eggs of GM Aedes aegypti.
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DEPT. OF ANALYTICAL CHEMISTRY
Method development & Validation
Chemical composition studies
Formulation analysis
Characterisation studies
e-Fate studies in plant, water and soil and
Metabolite identification
Chemical equivalence studies
Storage stability studies, Stability to metal and metal ions
Dissipation studies and Leaching in soil,
Bioaccumulation in fish
Kinetics and Hydrolysis
Adsorption/Desorption,
BOD/COD determination
Biodegradability studies
Explodability and Flammability studies
Evaluation of Biocide products
Physicochemical studies – technical/formulations
Multisite residue studies
Residue studies in food and agricultural products, biological and tissues
Carbon/Nitrogen Transformation Test
Rain fastness studies
Container compatibility
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DEPT. OF RADIO ISOTOPE LABORATORY This new facility was started to conduct environmental fate,
metabolism, dissipation and other related studies using C14 and Tritium Radio labeled compounds
Laboratory space greater than 3000 sq. ft. includes separate TSC and Archival room,
Personnel safety equipment includes contamination monitors, personal safety badges and restricted access.
BARC trained on Site Radiation Safety Officer .
Liquid Scintillation Counter - 2900TR,
HPLC- flow through ß Ram detector with fraction collector, Beckman cooling centrifuge
R.J.Harvey Biological Oxidizer,
TurboVap Nitrogen Evaporator, Vacuum rotary evaporator
Horizontal shaker, BOD Incubators, Deep freezer, Sartorius balance, fume cup-board etc.,
The Lab was approved by AERB, BARC, Mumbai to use radiolabelled compounds in various testing.
DEPT. OF GENETIC TOXICOLOGY Ames assay
In vitro chromosomal aberration assay – human lymphocyte,
In vivo chromosomal aberration assay – mouse/rat
In vivo micronucleus assay
Mouse lymphoma assay – L5178Y TK+/- cells
Sister chromatid exchange – human lymphocytes
Cytotoxicity assay using cell lines
In vitro micronucleus assay
Comet Assay
Dominant Lethal test – rat/mouse
Cultures sourced from International suppliers-ATCC/Mol Tox
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DEPT. OF BIOTECHNOLOGY
1. Detection of genetically modified crop
• DNA typing
• Detection of specific protein
2. Detection of microbial biocide agents in commercial formulation
and in test animals
3. Detection of specific protein in recombinant drugs
4. Thermal stability and pepsin digestibility of GM foods
INFORMATION TECHNOLOGY AND SYSTEMS
Windows 2008 Server is being
used for the LAN
Involves in maintaining electronic
data following OECD guidelines
STATISTICAL ANALYSIS
A separate statistics department exists.
Statistical methods for planning experiments, obtaining data, and organizing, analyzing, interpreting, summarizing, presenting and drawing conclusions for research requirements.
NCSS 2007 and TOXSTAT~3.5 are being used for the statistical
analysis of data
LIBRARY/REFERENCE
We recognize the importance of having readily available, reliable information. Our sponsors rely both on the quality of our products and our expertise in biotransformation to help them with their research need.
RESEARCH ACTIVITIES IIBAT scientists, in addition to performing regulatory studies, are also engaged in the following basic research programmes;
1. Leaching and dissipation of pesticides, impact assessment 2. Photocatalysis 3. Synthesis and Pharmacological Screenings 4. Bioaccumulation 5. Residue studies in Biological and environmental samples 6. Endocrine disruptor function of chemicals 7. Toxicity and mutagenicity of carbon and silver nano materials 8. Juvenile toxicity of pesticides 9. Toxicity and chemistry of obsolete and expired pesticides 10. Transplacental carcinogenicity of chemicals
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11. Transplacental genotoxicity of chemicals 12. Toxicity of ayurvedic/sidhha/herbal medicines 13. Toxicity and biocompatibility of medical devices/implants 14. Free radicals and oxidative damage due to chemicals 15. Toxicity and possible benefits of herbal cigarettes 16. Cytogenetic and molecular genetic studies in human neoplasia 17. Development of in vitro alternatives in chemical toxicity
predictions 18. Neurobehavioral and developmental Neurotoxicity due to
pesticides. 19. Possible utility of nano particles in decontamination of
pesticides in water 20. Response of stem cell to toxicants.
NEW RESEARCH INITIATIVES Currently staffs are working in validating the following methods
1. Establish toxicity measurements to endocrine systems
2. Use stem cells for cytotoxicity and genotoxicity studies
3. Dermal penetration, photosensitization studies using cell-lines and other models
4. Developing models for toxicity assessment of engineered nano materials
5. Use of nano materials for alleviating toxicity of pesticides and decontamination
SCIENTIFIC ADVISORY COMMITTEE
IIBAT is guided by the following advisory committee; constituted with eminent scientists;
1. Institutional Animal Ethics Committee (with nominees from CPCSEA of Govt. of India).
2. Institutional Biosafety Committee (with nominee from DBT of Govt. of India).
3. Scientific Advisory Committee-with experts from Govt. Institutions of India.
4. Institutional Ethical Committee
GOVERNMENT OF INDIA-PERMISSIONS/LICENSE
• Import of animals
• Breeding and Experiments on small and large animals
• Import of chemicals/reagents, equipments and cell lines
• Import of seeds
• Import of radioactive chemicals/reagents
• License to conduct experiments using radiolabelled compounds LIFETIME ACHIEVEMENT AWARD Dr. P. Balakirshna Murthy received Lifetime achievement award in
Toxicology by the Society of Toxicology, India. This award was given
away by Honourable Minister of Health of Government of India - Shri.
Gulab Nabi Azad at the inaugural function of the annual meeting
of STOX held on 9th December, 2010 at New Delhi.
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SCIENTIFIC ADVISORS IIBAT staff received training from the following experts as a part of ad-hoc programmes;
Dr. Robert Maronpot, Former Head, NIEHS, USA
Dr. Monique Y.Wells, Consultant Pathologist, IIBAT
Dr. Nancy Everds, USA.
Dr. Angela King-Herbert , NIEHS, USA
Mrs. Ruth Clark, Rivendell, UK
Dr. T.S. Kumaravel, Toxicologist-Consultant, UK
Dr. Peter Mann, EPL, USA
Dr. Chris Gardiner, USA
Dr. David M. Moore, Asst. VP & Director, Research Compliance Office, USA
Dr. Ranold A. Herbert, NIEHS, USA
Dr. K. Venkaiah, Deputy Director, Biostatistics, National Institute of Nutrition (NIN), Hyderabad, India
INTERNATIONAL REPRESENTATION The following is a partial list showing participation of IIBAT scientist in various international scientific meetings/workshops; EUROTOX 2011 CIPAC 2010 and 2011 SOT-2010 and 2011 IWGT -2009 Workshop, Switzerland ICEM – 2009, Conference, Italy CIPAC/FAO/WHO meeting - 2009, El Salvador IUTOX-Europe, 2008 EUROPOLA 2006 EUROTOX 2007 UK IRDG-Annual meeting 2006
SOCIETAL COMMITMENTS OF IIBAT Provide employment to local people and encourage women and weaker section of society to uplift their living standards
Medical support to local people by organizing camps with experts
Adopt poor students to support education
Organize planting of trees/medicinal plants to preserve ecosystem
Reduce carbon emission and use electrical appliances to reduce energy expenditure.
Help local farmers in agricultural practices.
To train Self Help Group people in employment opportunities.
Students projects
RECENT PUBLICATIONS Potential Cell line Toxicity of Environmental Nanoparticles M. Durga, Francis Arul Prakash, Abbu Rajasekar, P. Balakrishna Murthy, T. Devasena International Research Journal of Pharmacy, 2012, 3 (1); P.145 – 149 Oxidative stress and inflammatory responses of rat following acute inhalation exposure to iron oxide nanoparticles. Srinivas, A., Jaganmohan Rao, P., Selvam, G., Goparaju, A., Balakrishna Murthy, P., Neelakanta Reddy, P. Hum. Exp. Toxicol (In Press). Investigations on the photolysis of imazethapyr in aqueous solutions under direct sunlight by HPLC- UV and LC-MS/MS-ESI - Applications of green alga as a potential biomarker. Velmurugan Pandiselvi, Sivanandam Sathiyanarayanan, Sornam Ayyappan and Atmakuru Ramesh. International Journal of Chemistry Research, 2012, 3 (1), 45-51. (ICV – 3.38)
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MicroRNA-205 downregulation targets FADD-biomarker for tumorigenesis in rat hepatocellular carcinoma and thyroid adenoma tissues; Parvathi MVS, Balakrishna Murthy P - Adv Bio Tech: 11(7):19-21 Toxicity assessment of Swaskuthar Rasa Bhasma on Lemna gibba. M. Seenivasan, G. Chandrasehar, S. Ayyappan, K. Krishna Varma, G. Sathyavani and P. Balakrishna Murthy. Journal of Herbal Medicine and Toxicology (JHMT): Vol.6, Issue 1. 2012. Isolation and characterization of cellulose nanofibers from the aquatic weed water hyacinth- Eichhornia crassipes. Carbohydrate Polymers 87 (2012) 1701-1705. Marimuthu Thiripura Sundari, Atmakuru Ramesh. Catalysis of cellulose – New perspectives in preparation of biogenic material. Recent patents on catalysis, 1, 2012, Article in press. Marimuthu Thiripura Sundari, Atmakuru Ramesh*. Determination of Dithiocarbamate Residues in Aquatic Tox Medium By Gas Chromatography Electron Ionisation Mass Spectrometric Method; Gundoju Suresh, Perumal Elumalai Ravi and Atmakuru Ramesh - Journal of Pharmacy and Chemistry, Volume:5; Issue:4; October-December 2011 Photocatalytic decontamination of Halosulfuron-Methyl residues on soil surface using Fe doped TiO2 nanoparticles under direct sun light. Kousalya S, Sathiyanarayanan S and Ramesh A. Journal of pharmacy and chemistry, 2011, 5 (4), 3-7. Toxicity effect of expired pesticides to freshwater fish Labeo rohita, G. Satyavani, R.A. Gopi, S. Ayyappan, P. Balakrishna Murthy and P. Neelakantareddy. Journal of Agriculture and Environment, Vol. 12, June, 2011. Srinivas, A., Jaganmohan Rao, P., Selvam, G., Balakrishna Murthy, P., Neelakanta Reddy, P., Acute inhalation toxicity of cerium oxide nanoparticles in rats. 2011 Toxicology Letters, 205, 105-115.
Surekha, P., Srinivas, A., Selvam, G., Goparaju, A., Neelakanta reddy, P., Balakrishna Murthy, P., Acute toxicity study of nano zinc oxide in Sprague Dawley rats through oral route. Toxicology and Industrial Health, 2011. DOI: 10.1177/0748233711420473 Surekha, P., Sairam Kishore, A., Srinivas, A., Selvam, G., Goparaju, A., Neelakanta reddy, P., Balakrishna Murthy, P., Repeated dose dermal toxicity study of nano zinc oxide with Sprague Dawley rats. Cutaneous and Ocular, 2011 Toxicology (In Press) Aadarsh Prasanna, Deepa.V, Balakrishna Murthy.P, M.Deecaraman, Sridhar.R and Dhandapani P. Insoluble phosphate solubilization by Bacterial strains isolated from Rice rhizosphere soils from Southern India. International journal of soil science., 2011, 6(2), 134-141. Optimization of Callus Induction and Regeneration in Swarna masoori rice cultivar. Aadarsh Prasanna, P. Balakrishna Murthy, M. Deecaraman and R. Sridhar. Research journal of Agriculture and Biological Science, 2010, 6(6); 917-922. Efficient Phosphate solubilization by fungal strains isolated from Rice-rhizosphere soils for the phosphorus release. Deepa.V, Aadarsh Prasanna, Balakrishna Murthy.P, M.Deecaraman, Sridhar.R. Research journal of Agriculture and Biological Science, 2010, 6(4); 487- 492. Moringa oleifera Lam leaves prevent cyclophosphamide – induced micronucleus and DNA damage in mice. Sathya.T.N, Aadarsh.P, Deepa.V, Balakrishna Murthy.P. 2010. International Journal of Phytomedicine, Accepted, In press DNA damage caused by a textile dye (ACID RED) in coelomycetes of earthworm, Eisenia fetida.Sathya T.N., Deepa.V. Sunil Dutt. M and Balakrishna Murthy.P. 2010. Australasian journal of Ecotoxicology Accepted, In press Efficient phosphate solubilization by fungal strains isolated from rice-rhizospere soils for the phosphorus release.Deepa.V, Aadarsh prasanna, Balakrishna Murthy.P and R.Sridhar. 2010.Research Journal of Agricultural and Biological sciences. Accepted, In press.
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A comparitive analysis of chromosomal aberrations in cultured human lymphocytes due to fluoroquinolone drugs at different expression periods, M. Anupama, J.P. Seiler, P.B. Murthy, Archives of Toxicology, Springer Berlin, DOI 10.1007/s00204-009-0509-9 (Link date : January 04, 2010) Assessment of the dermal and ocular irritation potential of Multi -walled carbon nanotubes by using In vitro and In vivo methods A. SairamKishore, P. Surekha, P. Balakrishna Murthy Toxicology Letters 191 (2009) 268-274 Genotoxicity evaluation of certain Bhasmas using Micronucleus and Comet assays - T.N. Sathya., Balakrishna Murthy Ph.D., N.V. Vardhini.: The Internet Journal of Alternative Medicine 2009: Volume 7 Number 1 P. E. Ravi, P.E.Thiruganan* and A. Ramesh, A Capillary Gas-Chromatography Mass Spectrometry Electron ionisation method for the Determination of Eleven Chlorinated Herbicides in Whole Blood and Serum - Dissipation Kinetics, Journal Of Chromatography Method Development 1,1-9(2009). S. Sathiyanarayanan, P.E. Ravi and A. Ramesh, Applications of Zinc Oxide Nanorods as Photocatalyst for the Decontamination of Imidacloprid and Spirotetramat Residues in Water, The Open Catalysis Journal, 2, 37-45(2009). P.E. Ravi and A. Ramesh, A Gas Chromatography Mass Spectrometry Method For The Accurate Determination Of Mancozeb And Metiram (As Cs2) In Different Fungicide Formulations. CIPAC Symposium 2009. Hen Egg Chorioalantoic Membrane Bioassay – An In vitro alternative to Draize Eye Irritation Test for Pesticide Screening, A.S. Kishore, P.A. Surekha,P.V.R Sekhar, A. Srinivas, P. Balakrishna Murthy, International Journal of Toxicology Vol.27, Issue 6 Nov.2008, 449- 453 New Approaches in Toxicology Evaluation of Chemicals - P. Balakrishna Murthy : Ins Pest Magmt. & Envl. Safety, (Suppl.) 4, Vol. I, pp. 39-44, 2008.
Expression of NF 2 Gene in Vestibular Schwannoma meningioma in Indian patients - Sangeetha VV Naveen, Yogandhan K., Daniel Rajesh Babu and Balakrishna Murthy P. : Journal of Genetic Toxicology, Vol.1 No.1(Jun 2008). Brain Lesion in a Wistar rat - Encephalitozoonosis - Yogeshkumar V. Murkunde, P Kalaiselvan, S. Vijayakumar, K. Hemalatha, Robert R. Maronpot, Ronald A. Herbert, Monique Y. Wells : Lab Animal Volume 37, No.9, September 2008. Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes, M. Anupama, S.S. Murugan, P. Balakrishna Murthy, Food Chem. Toxicol. 2008;46:3351-3353. Larvicidal activity of neem and karanja oil cakes against mosquito vectors, Culex quinquefasciatus (Say), Aedes aegypti (L.). and Anopheles stephensi (L.) - R. Shanmugasundaram, T. Jeyalakshmi, M.Sunnil Dutt and P. Balakrishna Murthy : J. Environ. BioL 29(1), 43-45 (2008). Lambda-cyhalothrin induced changes in nucleic acid dynamics in freshwater catfish, Clarias batrachus - R. Saravanan, K. Revathi* and P. Balakrishna Murthy:J. Exp. Zool. India Vol. 10, No. 2, pp. 385-389, 2007. Adsorption and degradation of sulfosulfuron in soils – Maheswari, S.T. and Ramesh, A. : Environmental monitoring and assessment 2007; 127:97 to 103, Springer. Toxicological studies on snake venom-specific antibodies (IgY) from chicken egg–Manjula, J., Kusum, P., Sairam, A. K., Murthy, P. B., and Subbarao, P. V.: Journal of Cell and Tissue Research Vol. 6 (2) 733-738 (2006). Reduced susceptibility to deltamethrin and kdr mutation in Anopheles stephensi Liston, a malaria vector in India -V.Gayathri and P. Balakrishna Murthy : Journal of the American Mosquito Control Association (Accepted for publication).
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Identification of residues of sulfosulfuron and its metabolities in subsoil – dissipation kinetics and factors influencing the stability and degradation of residues from topsoil to subsoil under predominant cropping conditions – Dr. Atmakuru Ramesh, Perumal Elumalai Thirugnanam and Sivanandam Sathiyanarayanan : Journal of Environmental monitoring and assessment (Accepted for Publication). Simultaneous determination of Eight 1,3,5 – triazole herbicides in ground water and soil by GC-MS EI using selective in Monitoring Method – Scholars presentation. Perumal Elumalai Ravi and Atmakuru Ramesh : Journal of 10th ISMAS Triennial International Symposium on Mass Spectrometry , RS-1 page no.64. Cytogenetic analysis of 52 Indian patients with de novo myelodysplastic syndromes- A comparative analysis of results with reports from Asia: Amirtha Ganesh, Dakshinamurthy. Nicolas Novitzky, Raghva Bharadwaj, Murthy, Balakrishna Murthy Prakhya – British Journal of Haematology. 2005-Vol No.84, Pages:298-303. Management of Tomato collar rot caused by Sclerotium rolfsii by antagonistic microorganisms – R. Balasubramanian, G. Chandrasehar, S. Ayyappan and P. Balakirishna Murthy: Journal of Ecobiology 15(3)2005: Page no.261-264.
D.Sc. PROGRAMMES The following senior staff were awarded D.Sc. degree in Biomedical sciences. Name Year University Dr. P. Balakrishna Murthy 2000 University of Madras
Dr. A. Ramesh 2009 The Tamil Nadu Dr. MGR Medical University
Ph.D. PROGRAMMES Due to recognition by universities, full time Ph.D programmes are offered in the following faculties; Chemistry
Entomology Biotechnology Toxicology Weed science Botany-plant pathology
So, far nearly 52 students were awarded Ph.D by the University of Madras and Dr.M.G.R.Medical University. The following universities have recognized IIBAT to guide scholars for Ph.D.
University of Madras
Dr. MGR Educational and Research Institute
Anna University
Bharathiar University
Manonmaniam Sundaranar University
SV University, Tirupathi
TNAU, Coimbatore
Andhra University , Visakhapatnam
MGR Medical University, Chennai
Meenakshi University, Chennai
LIST OF MAJOR EQUIPMENTS
ANALYTICAL CHEMISTRY
Name of the Instrument Make / Model
Differential Scanning Calorimeter DSC Q20
Gas Chromatograph Agilent technologies 7890A / G 3440A
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Melting Point Apparatus DIGITAL
Shimadzu UV-VIS Spectrophptometer
UV-1601
Shimadzu FTIR – 8400S Shimadzu FTIR 8400S
Shimadzu GC-2010 GC-2010 AF
Shimadzu RF-10AXL HPLC-RF (LC-10AT vp,SCL-10A vp TO-10AASVP and RF-10AXL)
Gas Chromatograph GC-14BPF
GC-17A GC-17AAF
Shimadzu GC MS GC MS-QP5050A
Shimadzu HPLC SPD-20A,CTO-20A,LC-20AT
LC-MS/MS Agilent 1200 Series HPLC Bruker HCT Plus LC-MS/MS
Shimadzu HPLC PDA Detector
LC-20AT, SPD-M20A,CTO-20A, CBM-20A
Shimadzu HPLC LC-20AT, SPD-20A,CTO-20A
Agilent HPLC 1200 Series 1200 SERIES
RADIO ISOTOPE
Name of the Instrument Make / Model
Liquid Scintillation Counter,
Perkin Elmer Model- TriCarb 2900TR with QuantSmart TM Software.
Cooling centrifuge, Beckman Coulter Model- Allegra 25R
HPLC - UV Detector and B-Ram Detector
Shimadzu -Prominence UFLC Series IN/US system
Biological Oxidizer , R.J. Harvey model- OX-700
Deep Freezer Hitech
Rotary evaporator, Hahn Shin Model-HS2005S
Nitrogen Evaporator Turbo Vap
Caliper Life Sciences - Model- LV Work station
DCM CM710P
TOXICOLOGY Name of the Instrument Make / Model
Non invasive Blood pressure System
Biopac system
ECG and NIBP Data acquisition unit
Biopac system
Activity Cages UGO Basile
Responder-X System Columbus Instruments
Gripstrength Meter UGO Basile
Gripstrength Meter Mark 10 Corporation
Wright Dust Feeder CH Technologies, USA
Dust Generator CH Technologies, USA
Collison Nebuliser CH Technologies, USA
Data Logger R.S. Enterprises, Pune
PM 100 BALLMILL Retch, Germany
PATHOLOGY
Name of the Instrument Make / Model
Tissue embedder 1 Shandon Histocentre 3
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Tissue processor Tissue-Tech VIPTM 5 (Sakura, Japan) Leica
AutoStainer & Auto coversliiper
Leica
Biochemistry Fully Automatic Analyzer
Human -Humastar 300
Hematology Fully Automated Analyzer
Bayer/Advia 120
Electrolyte Analyzer Human - Humalyte
Urine Analyzer Bayer/Clinitek STATUS
Trinocular microscope Nikon Corporation, Japan Carl Zeiss
Stereo Microscope with imager
Carl Zeiss
GENETIC TOXICOLOGY Name of the Instrument Make / Model
Inverted Microscope Nikkon, TS 100
Trinocular microscope Nikon Corporation, Japan
CYTOSPIN 4 Thermo Electron Corporation
Biophotometer EPPENDORF
Binocular Microscope Carl Zeiss
Innova CO2 incubator Innova CO-170, New Brunswick Scientific
Schuett Biotech De. Count 3.081 502 Schuett-biotech GmbH, Germany
BIOTECHNOLOGY
Elisa Reader Stat Fax 2100 Fluorescence - Trinocular microscope with imager
Carl Zeiss
ACCEPTANCE OF TEST REPORTS IIBAT reports were accepted by the following national and international regulatory bodies; International US EPA,US FDA, AUSTRALIA, UK, GERMANY, SPAIN,
FRANCE,ITALY AND OTHER EU MEMBERS,
WHO , Latin American Countries-Ex. Argentina
National CIB-RC, RCGM/DBT, DCI LIST OF GOVERNMENT OF INDIA FUNDED ADHOC PROJECTS /RECENTLY COMPLETED AND CURRENTLY IN PROGRESS ICAR : Adsorption, Degradation and Biological
Interactions of Certain Herbicides in Soil
ICAR : Behaviour of Sulfosulfuron in sub-soil under the influence of wheat cropping system – Identification and quantification of potential transformation products responsible for the phytotoxicity and their bio-accumulation in fish
DBT : Cellulose nanofibers from aquatic weeds
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AGENCIES WHICH SUPPORTED RESEARCH AT IIBAT The following is a partial list of international and national funding agencies which supported research, with broad area of work
International
USDA, USA - Entomology
British Geological Survey, UK - Pesticide in ground water
WHO - Toxicity studies
National CSIR - Entomology
DST - Toxicity studies
ICMR - Malnutrition and mutagenesis, cancer genetics
ICAR - Bioaccumulation of Herbicides And their mobility
DBT - Nano chemistry
BRNS - Oncogene activation-radiation exposure
DRDO- Toxicology
LIST OF NATIONAL AND INTERNATIONAL TRAVEL GRANTS AWARDED TO SCIENTISTS OF IIBAT
1. Novartis, Switzerland
2. IUTOX
3. INSA, India
4. DST, India
RECOGNITIONS & COLLABORATIONS
Ministry of Science & Technology, Government of India, DSIR (R&D).
Madras University, Chennai
The Tamil Nadu Dr. MGR Medical University, Chennai
Tamil Nadu Agricultural University (TNAU), Coimbatore
Anna University, Chennai
Tamil Nadu Veterinary and Animal Sciences University, Chennai
Sri Venkateswara University, Tirupathi, Andhra Pradesh
The external links for collaborative research through international
agreements include Research Institute of Nuclear Medicine and
Biology, Hiroshima University, Japan
QUALITY ASSURANCE UNIT
Well established independent quality
assurance unit with high level of
competent and professional auditors
covering the auditing system as per the
principles of GLP.
Management
The affairs of the Institute are managed as per the rules prescribed by
the Registrar of Societies. The Executive Council of IIBAT consists
of Chairman, President, Treasurer, Vice President, Members and a
Director cum Secretary.
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IIBAT Facilities – An Overview Admin
Department of Toxicology
Department of Biotechnology
Analytical Chemistry
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Radioisotope Lab
Clinical Pathology
Pathology
Dept. of Genetic Toxicology
Prompt submission of quality research test reports Reliable and dependable test data Professional competence and assiduity Utter confidentiality of information
For further information, please contact: The Director, IIBAT, Padappai – 601 301, Kancheepuram Dist., Tamil Nadu, India. Phone: +91-44-27174246/27174266 Fax: +91-44-27174455 E – Mail : [email protected]
