BioXplore Final Conference December 4th, 2014

Jerusalem Israel

BioXplore final Progress Review

Hadassah Academic CollegeGili Joseph

Screens-To-Nature (STN)1. Antibacterial2. Antifungal3. Anthocyanine4. Flat worm Lethality Assay5. Protozoa Lethality assay6. Round Worm Lethality Assay7. Protease assay8. Protease Inhibitor Assay9. Glucosidase Assay10.Glucosidase Inhibitor Assay11. Planaria Regeneration Assay12. Antioxidant

Activity 0: Kick- off Meeting January 2012

Activity 1: First BioXplore workshop in Israel February 2012

Activity 2:First International workshop in Israel, April 2012

Working hard in the field…

Activity 3-4: Mapping the Local Flora,

Performing STN in the Field

Mt. Hermon

Hachula valley

Mount Carmel

Habonim Beach

Palmachim beach

Jerusalem area

Negev Desert

Judean Desert

Negev Desert

Judean Desert

Zichron Yaacov- Mt. Carmel

Einot tzookimDead Sea

Israeli group in the field

Mt. Hermon

Hachula Valley

Activity 5: Dissemination- BioXplore web site

Israeli results:(Activity 4,5,6)

Published in:Gili Joseph, Mina Faran, Ilya Raskin, Mary Ann Lila and Bertold Fridlender Medicinal Plants of Israel: A Model Approach to Enable an Efficient, Extensive, and Comprehensive Field Survey. J Biodivers Biopros Dev 2014, 1:3

Distribution of the 1029 plant samples( 628 plant species) collected among plant families

Plant FamilyNumber

of samples

Percent of all samples screened

1 Asteraceae 117 11.7%2 Fabaceae 105 10.5%3 Lamiaceae 79 7.9%4 Brassicaceae 62 6.2%5 Liliacee 39 3.9%6 Apiaceae 35 3.5%7 Boraginaceae 30 3.0%8 Poaceae 26 2.6%9 Caryophyllaceae 25 2.5%10 Chenopodiaceae 25 2.5%11 Ranunculaceae 24 2.4%12 Scrophulariaceae 21 2.1%

13–2513 plant families, each

representing <2% of total 177 17.7%

26-8560 plant families, each

representing <1% of total 223 22.3%

Distribution of plant parts extracted

Plant samples with the highest level of activity for each test performed.

Number of activities in the same plant sample

Plant samples showing 3–4 anti-infectious activities Antibacterial, antifungal, flatworm lethality, roundworm lethality, anthelminthic, and protease inhibitor

activities

Genus species FamilyBeta vulgaris ChenopodiaceaeCarrichtera annua BrassicaceaeConyza canadensis AsteraceaeCynoglossum nebrodense BoraginaceaeDeverra tortuosa ApiaceaeEpilobium hirsutum OnagraceaeFraxinus syriaca OleaceaeHypericum triquetrifolium HypericaceaeLotus palustris FabaceaePistacia khinjuk AnacardiaceaePistacia lentiscus AnacardiaceaePistacia palaestina AnacardiaceaeProsopis farcta MimosaceaeRanuncalus marginatus RanunculaceaeRhamnus libanotica RhamnaceaeRhus coriaria AnacardiaceaeRosularia libanotica CrassulaceaeRuta chalepensis RutaceaeSarcopoterium spinosum RosaceaeTamarix aphylla TamaricaceaeTamarix nilotica TamaricaceaeTamarix tetragyna Tamaricaceae

Relationship between climate conditions and bioactivities

Plants showing antifungal activity in dilution of 1:20 (25mg/ml)

Part of plant Plant name in Hebrew

Plant name

Above ground שרועה לוניאה Launaea nudicaulis

leaves בשרני טיון בן Limbarda crithmoides

leaves הגבישים אהל Mesembryanthemum crystallinum

fruit הבורסקאים אוג Rhus coriaria

flower היאור אשל Tamarix nilotica

Plants showing anthocyanine activity in dilution of 1:20 (25mg/ml)

Part of plant Plant name in Hebrew

Plant name

flower מצויה כלנית Anemone coronaria

leaves אטלנטית אלה Pistacia atlantica

Fruit החורש מורן Viburnum tinus

Plants showing Glucosidase Inhibitor activity in dilution of 1:20 (25mg/ml)

Part of plant Plant name in Hebrew

Plant name

Above ground מצרי אשבל Ammophila arenaria

fruit מצוי חולות ידיד Ammophila arenaria

shoots הגינות מרור Sonchus oleraceus

roots צהוב סביון Senecio flavus

Plants showing protease Inhibitor activity in dilution of 1:20 (25mg/ml)

Part of plant Plant name in Hebrew

Plant name

leaves מרווני לוטם Cistus salviifolius

leaves המסטיק אלת Pistacia lentiscus

leaves(Fruit kills planaria)

הבורסקאים אוג Rhus coriaria

Two new assays were developed:

1. Anti cancer using yeasts model ( paper in preparation)

2. Anti viral assay using Herpes simplex virus

Sigal Matza-Porges, Kobi Eisen, Hadeel Ibrahim, Adva Haberman,Bertold Fridlender,Gili Joseph. A new antiviral screening method that simultaneously detects viralreplication, cell viability, and cell toxicity. Journal of Virological Methods 208 (2014) 138–143

Personnel Participating in BioXplore researchProject Coordinator: Prof. Bertold FridlenderProject Manager: Dr Gili JosephBotanists: Dr. Mina Faran, Dan Bolotin, Technician: Dr Galina Mengerisky

Students:Mahmud Abo Ras Orly dery Omri Duev Ahmed Abo Ras Sara Pinto Daria PetrovKobi Eisen Hadil Ibrahim Adva HabermanMeshel Nicola Gal Mamruth Odelia Ben HamuDuha Faha Keren Gavish

Thank you for your attention!

Dr. Gili Joseph Project Manager, BioXploreHadassah Academic College Jerusalem