in Himachal Pradesh

BIOTECHNOLOGY

Status Report2017

HIMACHALPRADESHSHIMLA­171002

ForewordThe biotechnology sector of India is highly innovative and growing. India is among the top 12 biotech

destinationsintheworldandranksthirdintheAsia­Pacificregion.Outofthetop10biotechcompaniesinIndia

(byrevenue),sevenhaveexpertiseinbio­pharmaceuticalsandthreespecialiseinagri­biotech.Bio­pharmais

thelargestsectorcontributingabout64percentofthetotalrevenuefollowedbybio­services(18percent),

bio­agri(14percent),bio­industry(3percent),andbioinformaticscontributing(1percent).Indiahasnodearthoftalentinbiotechnology,asanumberofinstitutions,bothgovernmentandautonomous,

providethenecessaryopportunitiesforthestudentsseekingtoobtainadegreeinthissector.TheGovernment

of Indiahasprovidedadequatescopeto thissectorbyproviding facilities forResearchandDevelopment

(R&D)inthefieldofbiotechnology.HimachalPradeshoffersahugepotentialasaHimalayanbio­resourcesbasedbiotechnologyhubthathouses

7.32 per cent of countries' bio­resources in the formof high value plantswith importantmedicinal and

aromatic properties. More than two dozen R&D Institutions/Department are engaged in innovations,

disseminationandpromotionofBiotechnologyinvariousareas.Inadditiontothisthereisalargepoolof

highlyskilledmanpowerinBiotechnologyintheStateproducedeachyearbyseveralUniversitiesandColleges

inPublicandPrivatesector.TheGovernmenthasnotifiedtheStateBiotechnologyPolicywithavisionand

objective to promote Biotechnology by supporting R&D Institutions in agriculture, animal husbandry,

environment,biodiversityconservationandutilizationthroughsettingupofbio­resourcesbasedindustriesin

theState. Allthiscombinedtogether,thereisaneedtohaveastrongconvergencewiththeGovernment­

Academia–IndustriestomovefasterintranslatingHimachalPradeshintothemostsoughtafterdestination

forbio­resourcesbasedbusinesshubinthecountry.ThemaideneffortoftheDepartmenttotakeoutacomprehensivereportonStatusofBiotechnologyintheState

ispraiseworthyandwouldserveasaneffectiveguidefortheBiotechnologysectorintheState.Thecompilation

ofsectorwisedetailsof theprojects,patents,achievements, facilitiesetc.ofvarious institutionsprovides

immensescopeforbiotechnologybasedentrepreneurstosetupstartupventuresintheState.Itisamatterof

pridethatthereareabout27InstitutionsinPublicandPrivatesectorsworkingforpromotionofBiotechnology

in the State beside educational institutions producing considerable graduates and post­graduates in

BiotechnologyintheState.IhopetheReportservesasausefulreferenceforallconcernedstakeholdersofBiotechnologyintheStatefor

planningandimplementingbiotechnologybasedprogrammesthroughscientificuseofitsinnovationsand

bio­resources.

AdditionalChiefSecretary(Env.,S&T)totheGovernmentofHimachalPradesh,Shimla­2

(Tarun Kapoor)

HIMACHALPRADESHSHIMLA­171002

Addl.ChiefSecretaryForest,EnvironmentScience&Technology

FoodCivilSupplies&ConsumerAffairsGovernmentofHimachalPradesh

Himachal Pradesh has more than 25 R&D Institutions which are dealing in various aspects of

Biotechnologyonadaytodaybasisandaregeneratingalargepoolofdataandinformationforits

applicationinindustriesanddailylifeofcommonman.Besidethis,alargenumberofhighlyskilled

manpowerinBiotechnologysectorisproducedeachyearintheState.Keepinginviewthesefacts,the

Department of Environment, Science&Technology has endeavored to publish a Status report of

BiotechnologyinHimachalPradeshsoastoprovidefirsthandinformationonBiotechnologytoallthe

stakeholdersintheState.TheinformationwassoughtfromalltheseR&DInstitutionsoftheStateand

basedontheinformationreceivedfromtheseInstitutionsthisreporthasbeencompiledinitspresent

form.IextendmysincerethankstoIHBT,Palampur,CPRI,Shimla,CSKHPKV,Palampur,Dr.YSPUHF,Nauni,

Solan, HPU, Shimla, Jaypee University,Waknaghat for providing valuable information on various

aspectsofBiotechnologyinatimelymanner;withoutthesameitwouldnothavebeenpossibleforusto

publishthisreport.IalsoextendmysincerethankstothescientificandsupportstaffofBiotechnologyDivisionofthe

Department of Environment, Science& Technology for their valuable contribution and efforts in

compilingtheReportandgivingitspresentform.Ifurtherputonrecordmysincerethankstoallthe

organisations/personnel for their valuable suggestions and guidance that has made this Report

possible.Needless to say, since this is the first attempt of the Department to bring out such Report on

Biotechnology,someerrorsandomissionsmayhavebeencreptin,whichmaypleasebebroughttothe

noticeofthisDepartment.

Director,DepartmentofEnvironment,Science&Technology,

Shimla­1

Acknowledgement

(Archana Sharma),

HIMACHALPRADESHSHIMLA­171002

DIRECTORDepartmentofEnviornment,Science&Technology

GovernmentofHimachalPradeshParyavaranBhawan,Shimla­171001

ContentsSr. Title PageNo.

1 BackgroundandDevelopments 1­2

2 GovernmentInitiatives 3­4

3 StateBiotechnologyPolicy 5­7

4 Objectives 8

5NameandaddressesofkeyStakeholderInstitutionsofBiotechnologyinHimachalPradesh

9

6 BiotechnologysectorsinHP 10

6.1 GreenBiotechnology 10

6.2 RedBiotechnology 11

6.3 WhiteBiotechnology 12

6.4 BiotechnologyEducationSector 12

7 BiotechnologyInformationsystemNetworkinHimachalPradesh 13

8 BIFCenters 14

9.1 PlantBiotechnologySector 14­16

9.2 AnimalBiotechnologySector 17

9.3 FoodBiotechnologySector 17

9.4 BiotechnologyEducationSector 18

9.5 ForensicBiotechnologySector 19

10 ResearchachievementsinBiotechnologybyvariousInstitutesofH.P. 20

10.1 InstituteofHimalayanBioresourceTechnology,Palampur 20­23

10.2 CentralPotatoResearchInstitute,Shimla­171001(HP) 24

10.3 CSKHimachalPradeshKrishiVishvavidyalaya,Palampur 25

10.4 Dr.Y.S.ParmarUniversityofHorticultureandForestry 26­27

10.5 HimachalPradeshUniversity,Shimla. 28

10.6 JaypeeUniversityofInformationTechnology,Waknaghat,Solan–H.P 29­30

10.7 DirectorateofMushroomResearch,Solan­H.P 31

11 Infrastructurefacilityindifferentbiotech.labsofdifferentinstitutesofH.P

32

12 ExpertsofBiotechnologyinHimachalPradeshfromvariousInstitutes 33­35

13 IssuesandChallenges 35

BIOTECHNOLOGY

APOPTOSIS

AUTORADIOGRAPHY

ATP

BIOINFORMATICS

CELL CLONE

DNA

GENOME

ZYGOTE

TISSUE

ANAEROBE

Background and Developments

TheBiotechnologysectorofIndiaishighlyinnovativeandisonastronggrowthtrajectory.Indiaisamongthetop12biotechdestinationsintheworldandranksthirdintheAsia­Paci�icregion.Indiahasthesecond­highestnumberofUSFoodandDrugAdministration(USFDA)–approvedplants,aftertheUSAandisthelargestproducerofrecombinantHepatitis B vaccine. Out of the top 10 biotech companies in India (by revenue), seven have expertise in bio­pharmaceuticalsandthreespecialiseinagri­biotech.Biopharmaisthelargestsectorcontributingabout64percentofthe total revenue followed by bioservices (18 per cent), bioagri (14 per cent), bioindustry (3 per cent), andbioinformaticscontributing(1percent).Indiahasemergedasaleadingdestinationforclinicaltrials,contractresearchandmanufacturingactivitiesowingtothegrowthinthebioservicessector.Indiahasnodearthoftalentinbiotechnology,asanumberofinstitutions,bothgovernmentandautonomous,providethenecessaryopportunitiesforthestudentsseekingtoobtainadegreeinthissector.TheGovernmentofIndiahasprovidedadequatescope to thissectorbyproviding facilities forResearchandDevelopment(R&D) in the �ieldofbiotechnology.

TheIndianbiotechindustryholdsabout2percentshareoftheglobalbiotechindustry.ThebiotechnologyindustryinIndia,comprisingabout800companies,isvaluedatUS$11billionandisgrowingataCompoundAnnualGrowthRate(CAGR)of20percent.ThegovernmenthastoinvestUS$5billiontodevelophumancapital,infrastructureandresearchinitiativesifitistorealisethedreamofgrowingthesectorintoaUS$100billionindustryby2025,asperUnionMinisterforScienceandTechnology,MrHarshVardhan.ThehighdemandfordifferentbiotechproductshasalsoopenedupscopefortheforeigncompaniestosetupbaseinIndia.

In1953,structureofDNAwasproposed,followedbytherevolutionarydiscoveryofrecombinantDNAtechnologythateventuallypavedthewayformodernbiotechnology.Withthediscoveryofrestrictedenzyme,itbecamepossibletoinsertforeigngenestobacteriain1973.Thistechniqueenabledproductionofhumaninsulinfrombacteriaandisalsoconsideredasthebirthofnewinnovativebiotechnology.Thislegacyofbiotechnologicalbreakthroughs,capableofchanginglives,isbeingcontinuedtilldate.

The Telangana state government's �lagship pharma and

biotechevent­BioAsia2017attractedinvestmentstothetuneofRs3,382crore(US$507.3million).

During the Vibrant Gujarat Global Summit­2017, 54 MoUs

worthRs5,022crore(US$736.1million)inthebiotechnologysectorweresignedby37companies.

SyngeneInternationalLtd,thecontractresearchservicesarm

ofBioconLtd,issettingupadrugdiscoveryanddevelopmentcenterinBengaluruforAmgenInc.,abiotechnologycompanybasedintheUS.

US­basedmedicaldevicesmakerBostonScienti�icCorp.plans

tomake India itsbiggest researchanddevelopment (R&D)hub outside the US, as it plans to develop devices such asstents,cathetersandpacemakersatitsGurgaonfacilityfortheAsiaPaci�ic,MiddleEastandAfricanmarkets.

Frost&Sullivanhassignedamemorandumofunderstanding

(MoU) with The Neotia University (TNU) to set upbiotechnologyparksinIndia.

BiotechnologygiantMonsantohasplannedtosetupaseed

plantinBuldhanadistrictofMaharashtra.

GlobalbeveragemajorPepsihasplannedtosetupanother

unit in the State of Maharashtra to manufacture mango,pomegranateandorange­basedcitrusjuice.

BiotechnologyIndustryResearchAssistanceCouncil(BIRAC),

the funding agency of Department of Biotechnology, hasplanned to allocate around Rs 100­200 crore (US$ 15­30million) for its biotech equity fund,which aims to provide�inancialassistancetobiotechandlifesciencesstart­ups.

Canada's Centre for Commercialisation of Antibodies and

Biologics (CCAB) has partnered with ZydusCadila to

manufactureantibody­basedcancertreatments.

Shantha Biotechnics Private Limited has started building a

facilitytomanufactureInsuman,aninsulinproducttotreatdiabetes.Frenchpharmaceutical companySano�iSA,whichacquired ShanthaBiotechnics in 2009 through its vaccinesdivision,Sano�iPasteurSA,isinvestingRs460crore(US$69million)tobuildthefacilitythat,atfullcapacity,willproduce60millionInsumancartridgesannually,companyexecutivessaid.

Hyderabad headquartered vaccine manufacturer Indian

Immunologicals Limited (IIL), part of the National DairyDevelopment Board, is sett ing up a new vaccinemanufacturingfacilityinPuducherryinvolvinganinvestmentofRs300crore(US$45million).ThisisthefourthfacilityforIIL,whichcurrentlyhastwofacilitiesinHyderabadandoneinOoty.

Bristol­MyersSquibbandSyngeneInternational,thecontract

research subsidiary of Biocon, have announced a �ive­yearextension of their drug discovery and developmentcollaborationinIndia.

The Bhabha Atomic Research Centre (BARC) through its

Centre for IncubationofTechnologies (BARCIT)has signedanMoUwithM/sVeenaIndustries,Nagpur,forincubationoftechnology forbiodegradableandedible �ilms for foodandpharmaceuticalspackaging.

AurobindoPharmaannouncedthatitsBoardofDirectorshave

approvedtheproposalforsettingupaJointVenture(JV)withTergeneBiotech, a vaccine development companybased inIndia. Tergene is currentlyworking on development of thePneumococcalConjugateVaccine(PCV)throughuseofnovelvaccine technology compressing time and cost, thereby,makingsuchvaccineavailableatanaffordablecost.

1

Government Initiatives

IntheUnionBudget2017­18,theDepartmentofBiotechnology(DBT)receivedRs2,222.11crore(US$333.31million),anincreaseof22percent,to continue implementing the department'snational biotech strategy and target increasingtheturnoverfromthesectorto$100billionby2025from$7billionin2016.

The Telangana government also inked anMoUwithPE�irmCerestratoexplorea'LifeSciencesInfrastructureFund'witha corpusofRs1,000crore(US$150million)tocreateasophisticatedmodularplugandplayinfrastructureforpharma,biotechandmedicaldevicesindustry.

Dr. HarshaVardhan, UnionMinister for Scienceand Technology & Earth Sciences, inauguratedIndia �irst cellulosic alcohol technologydemonstration plant developed throughindigenous technologywith capacity of 10 tonperday.

TheRegionalCentreforBiotechnologyBill,2016hasbeenapprovedinLokSabha,whichaimstoprovidethestatusofnationalimportancetotheRegionalCentreforBiotechnology,forfacilitatingtransfer of technology and knowledge andmakingitahubforbiotechnologyexpertiseinthe

Asianregion.

TheGovernmentofIndiahasrelaxednormsforimportandexportofhumanbiologicalsamples,doing away with import licences or exportpermits,inanefforttoimprovetheeaseofdoingbusinessintheindustry.

The Government of India has s igned acooperation agreement with the EuropeanMolecular Biology Organization (EMBO) tos t reng then s c i en t i � i c i n te rac t i on andcollaborative research between India andEurope.

The Government of India aims to scale­up thenumberof start­ups inbiotechnologysector to1,500­2,000overnext two to threeyears from500currently.

TheGovernmentofIndiaisplanningtolaunchaventurecapitalfundofRs1,000Crore(US$150m i l l i o n ) u n d e r t h e d e p a r t m e n t o fpharmaceuticals, to support start­ups in theresearchanddevelopmentinthepharmaceuticalandbiotechindustry.

TheGovernmentofKarnatakaplanstoraiseRs50crore(US$7.5million) forabiotechnology­

2

dedicated fund in addition to the existingSemiconductor Fund of Rs 100 crore (US$ 15million) in order to engagewith the emergingtechnologyandbiotechspaceinthestate.

CSIR­Institute of Himalayan BioresourceTechnology(CSIR­IHBT)hassignedanMoUwithPhytoBiotechtoformalisetransferoftechnology,for production of unique autoclavable superoxidedismutase(SOD)enzyme,usedincosmetic,food and pharmaceutical industries for endapplications.

DBThasannouncedtheIndo­AustralianCareerBoosting Gold Fellowships underwhich it willsupport the researchers to undertake acollaborative research project at a leadingscienceinstituteoruniversityinAustraliaforaperiodofupto24months.

DBThasallocatedRs4.6crore(US$0.68million)totheUniversityofAgriculturalSciences(UAS)to

support a national multi­institutional projecttitled'Avaluechainonjackfruitanditsproducts'.

Underthe12thFiveYearPlan,theGovernmentofIndiaplanstostrengthenregulatoryscienceandinfrastructure, which involves setting up ofBiotechnology Regulatory Authority of India(BRAI) and a central agency for regulatorytestingandcerti�icationlaboratories.

ForeignDirect Investment (FDI)up to100percentispermittedthroughtheautomaticrouteforGreen�ieldandthroughthegovernmentrouteforBrown�ield,forpharmaceuticals.

National guidelines have been laid down toensure that researchwith human stem cells isconducted inaresponsibleandethicalmannerand complieswith all regulatory requirementspertainingtobiomedicalresearchingeneralandofstemcellresearchinparticular.

3

State Biotechnology Policy

Misssion Statement

Vision

Objectives

Strategies

ToconvertHimachalPradeshintoaprosperousHimalayanBio­businessHubwithBiotechnologyasoneoftheengines

of growth through scienti�ic and technological empowerment of human resource for enhancing ef�iciency,

productivity,costeffectiveproducts,processesandtechnologies.

Promotion of Biotechnology through its application in agriculture, animal husbandry, horticulture, medicine,

environment,biodiversityconservationandutilizationandbio­industrialdevelopmentintheState.

TomakeHimachalapreferredandgloballycompetitivedestinationfordevelopmentofBTproducts,processesand

services.

ToupgradeinfrastructuralsupporttoR&DandEducationalInstitutionstogeneratehighlyskilledhumanresource

inbiotechnology.

TosupportR&Dinpotentialareasofbiotechnology,includingagriculture,horticulture,animalhusbandry,human

health,environmentandindustry.

Toconserveandcommerciallyexploitbio­resourcesoftheStateforsustainabledevelopment.

Tocreateawarenessabout the investmentopportunities inbiotechnology,genomics,bioinformatics,biofuels,

contractresearch,etc.toentrepreneursandpromoteBiotechnologyindustrialinvestmentintheState.

ToprovidesuitableInstitutionalframeworktoachievetheobjectives.

Scienti�icandtechnologicalempowermentofhumanresourceforenhancingef�iciency,productivity,costeffective

products,processesandtechnologies

Identi�icationofareasforsustainablyharnessingtherichbioresourceavailableintheState.

Ensureaneffectivescienti�icbaseandmakestrategicinvestmentsinR&Dtosupportbiotechnologyinnovationand

economicdevelopment.

Patentingofresearch�indingsusingeffectivepatentingregimes.

Supportinginfrastructureforupscalingtheresearchforcommercialproduction.

4

Beside this, the Department will:

Area of Operation

Establishment of Biotechnology Parks

Outlineanumberofincentivesforsettingupofenterprisesbasedonlocalresourcesandcommercializationof

researchbase.

Promotionofindustriesformanufactureofendproductsbasedonbioresources.

ConservationofbioresourcesoftheState.

Institutional Framework to Achieve the ObjectivesTheDepartmentofEnvironment,Science&TechnologywillbetheNodalDepartmentintheStatethatwilldealwith

mattersconcerningbiotechnology.TheDepartmentwillpromoteappropriateresearchindifferentsectors,laymore

emphasison �ields likebioresourceutilization, conservationand industrialpromotion that are likely to create

employmentandwillmaintainnetworkingbetweencompanies,researchcentersandinstitutions.

TheregulatorymechanisminBiotechnologyshallbeinlinewiththatoftheActsandRulesasnoti�iedbytheCentral

GovernmentunderEnvironmentProtectionAct,1986underRuleforManufacture,Use,Import,ExportandStorage

ofHazardousMicro­Organisms,GeneticallyEngineeredOrganismsorCellsRules, 1989 forGMOs andproduct

thereof.TheseRulesshallbeexecutedbytheDepartmentofEnvironment,ScienceandTechnologyintheState.

DevelopmentofBiotechnology infrastructure forBT Industry in theStatewillbe facilitatedby the Industries

Department.TheStateGovernment,throughcollaborativepartnershipsandprovisionsoffacilities,proposesto

encourageandfacilitatetheprivatesector,indevelopingnecessaryBiotechnologyindustrialinfrastructuresuchas

BiotechnologyParksandothersupportinginfrastructureforresearch,training,testing,accreditation,etc.

State's varied regional characteristics such as geographic location agroclimatic conditions, distribution of

industries,academicinstitutes,researchcenteretc.providecomparativeadvantagetoparticularbiotechsector.In

viewofsuchcomparativeadvantagestheStateGovernmentproposestoencouragethedevelopmentofdedicated

clusters.Inthefollowingarea:

TheStateisalreadyintheprocessofsettingupaBiotechnologyParkatAduwal,Teh.Nalagarh,Dist.Solan.ItisproposedtosetupBiotechnologyParks/BTIndustrial.ClusterinHimachalPradeshinassociationwiththeprivatesectortoboostbiotechnologybasedventures.BiotechnologybasedandotherauxiliaryindustrieswillbelocatedintheseParks.ItisproposedtolocatetheseParksontheSolanandSirmourdistricts.

TheBiotechnologyPolicywillbeoperationalintheStateofHimachalPradesh

5

Biotechnology Task Force

R&D activities shall be supported by the Government by part or full funding of Himachal Pradesh speci�ic

biotechnologyresearch.

ATaskForcehasalreadybeenconstitutedtoadvisetheStateGovernmentonmakingbiotechnologyrelated policies and implementing them. The Task Force will also formulate norms for setting upbiotechnologybasedindustry.

HumanResourceDevelopment inBiotechnologysectorshallbesupportedbyenhancingcourse (curriculum)

developmentspecialHRDprogrammessupporttoBTstudentsandfacultynetworkingofBTinstitutionssupport

forinvitedtalksandexperiencesharingmechanisms.

Support institutions engaged in conservation of bioresource and its sustainable utilization inmanufacturing

sector.CoordinateandsupporttheR&DInstitutionstocreategenebanksofhighvaluebioresourceoftheState

besidespromotingscienti�iccultivationofendangeredplantshavinggreatcommercialimportance

6

Objectives

1) SurveyonuseandapplicationofBiotechnologyinvarioussectorsand2) Informationcollected/receivedfromvariousR&DInstitutionsoftheState.

Therefore,theoverallobjectiveofthisdocumentwastoprepareReportonBiotechnologyforHimachalPradeshandaswellastoproduceaproceedingswithexpertspapersandresolutions inordertoenhanceBiotechnologicalcapabilitiesof theState forallrounddevelopment.

BB

B

7

Name and Addresses of Key Stakeholder Institutions of Biotechnology in Himachal Pradesh

Sr.No. NameofInstitution AddressoftheInstitution

1DepartmentofEnvironment,Science&Technology,GovernmentofH.P.

ParyavaranBhawan,USClub,Shimla­171001.

2 HimachalPradeshUniversity SummerHill,Shimla­171005.

3 Dr.YSParmarUniversityofHorticultureandForestry Nauni,Solan,H.P173230.

4 CSKHPKrishiViswavidyalaya Palampur,Distt.Kangra,H.P.

5 StateForensicScienceLaboratory Junga,Dist.Shimla,H.P.

6 IndiraGandhiMedicalCollege Shimla,H.P.

7 StateCouncilforScienceTechnologyandEnvironment SDAComplex,Kasumpti,Shimla­171009,H.P.

8 DepartmentofAgriculture DirectorateofAgriculture,Shimla­171005,H.P.

9 DepartmentofHorticultureDirectorateofHorticulture,Navbahar,Shimla­171007,H.P.

10 DepartmentofAnimalHusbandryDirectorateofAnimalHusbandry,Shimla­171005,H.P.

11 DepartmentofForests Talland,Shimla­171002,H.P.

12 DepartmentofAyurveda SDAComplex,Shimla­171009,H.P.

13 CentralResearchInstitute Kasauli,Distt.Solan,H.P.

14 DirectorateofMushroomResearchCentre Chambaghat,Distt.Solan­173213,H.P.

15 CentralPotatoResearchInstitute Bemloe,Shimla­171001,H.P.

16 IndianAgricultureResearchInstituteAmartaraCottage,Tutikandi,Shimla­171005,H.P.

17 NBPGR,ResearchStationPhagli,Shimla­171003,H.P.

18GBPantInstituteofHimalayanEnvironmentandDevelopment

Mohal,Distt.Kullu,H.P.

19 IndianInstituteofTechnology Kamand,Distt.Mandi,H.P.

20 NationalInstituteofTechnology Hamirpur,H.P.

21 InstituteofHimalayanBioresourcesTechnology(IHBT) Palampur,Distt.Kangra,H.P.

22 Govt.PGCollege Dharamshala,H.P.

23 ShooliniUniversityofBiotechnologyandManagement Bajhol,Distt.Solan,H.P.

24 JaypeeUniversityofInformationTechnology Waknaghat,Distt.Solan,H.P.

25 HimalayanResearchGroup ChottaShimla,Shimla,H.P.

26 Industries&PrivateEntrepreneursBaddi,Kullu,Bilaspur,H.P.

27 Dr.RPMedicalCollege Tanda,Dist.Kangra,H.P.

8

Biotechnology sectors in HP

Plant Biotechnology Sector A number of research institutions such asgovernmental,semi­governmentalandprivateinstitutions/ their divisions are majorstakeholdersofplantbiotechnologysector inHP. Some of the major institutions of plantbiotechnology sector in the State is depictedbelow:

Environmental Biotechnology Sector

Dr.YSPUHF,Nauni

HPU,Shimla

IHBT,Palampur

GBPNIHESD,Kullu

DMR,Solan

NBPGR,Shimla

CPRI,Shimla

CSKHPKV,Palampur

HPU,Shimla

Dr.YSPUHF,Nauni

GBPNIHESD,Kullu

HFRI,Shimla

Ofthelistedinstitutions,fivehavebeenidentifiedaskeystakeholderinstitutionsinthissector

9

Biotechnology sectors in HP

6.2 Red BiotechnologyAnimal Biotechnology Sector The S t a te Depar tment and ve te r inarylaboratories/pharmaceuticals are the keyinstitutionsof thissector.Two institutionshavebeenidentifiedaskeystakeholderinstitutionsinanimal biotechnology sector of HimachalPradesh.

Forensic Biotechnology Sector StateForensicLaboratory(SFSL)istheonlykeyinstitutioninforensicbiotechnologysectorinHimachalPradesh.

Medical Biotechnology Sector A number of Medicine related institutions such asgovernment and private hospitals, pharmaceuticalcompanies, pathology laboratories, and universitydepartments are the stakeholders of medicalbiotechnologysector.Ofthelistedstakeholders,threeinstitutionswerefoundtobethekeyinstitutionsinthis sector. Name of the respective institutions areshown.

IGMC,Shimla

10

6.3 White Biotechnology

Industrial and Food Biotechnology Sector Ofthelistedinstitutions,sixhavebeenidentifiedaskeystakeholderinstitutionsinthissector.

DMR,Solan

Dr.YSPUHF,Nauni

HPU,Shimla

CPRI,Shimla

Bio-energy & Bio-conservationTechnologies

SixkeystakeholdershavebeenidentifiedwhichareinvolvedinR&Dactivitiespertinenttobio­energyandbioconversiontechnologies.

HFRI,ShimlaDr.YSPUHF,

Nauni

IHBT,PalampurNBPGR,Shimla

HPU,Shimla GBPNIHESD,Kullu

6.4 Biotechnology Education Sector

Eleveninstitutionshavebeenidentifiedaskey s t a keho l d e r i n s t i t u t i o n s i nBiotechnology Education sector inHimachalPradesh.

Dr.YSPUHF,Nauni

HPU,Shimla

IHBT,Palampur

CPRI,Shimla

Govt.PGCollege,Mandi

Govt.PGCollege,Dharamshala

GBPNIHESD,Kullu

GBPNIHESD,Kullu

HFRI,Shimla

CRI,Kasauli

JUIT,Solan

ShooliniUniversity,Solan

11

Biotechnology Information System Network in Himachal Pradesh

Sub­CentresinIndia

DistributionInformationCenters

RepositorytoInformationinrespective

Biotechnologyarea

DistributionInformationSub­Centers

AccessanddiffusionofInformation

acrossthenetwork

CSKHimachalPradeshKrishiVishavidyalaya

Dr.Y.S.ParmarUniversityofHorticulture&Forestry

HimachalPradeshUniversity,Shimla

Sub­

Centresin

Himachal

Pradesh

>

>

>

12

Bioinfomatics Infrastructure Facility for Biology teaching through Bioinfomtics

Present status of biotechnology under different sectors in Himachal Pradesh

9.1 Plant Biotechnology Sector

ThegoaloftheschemeistoexposeteachersandstudentstorealworldofscienceanduseofBioinformaticsin

solvinghardcorebiologicalproblems.Tutorialsareavailableonlinetohelpstudentsandteacherslearnhowto

navigatethoseresourcesthroughonlineandgatherdatathatcanbeappliedtooriginalquestions.Severalsites

providefreeandunhinderedaccesstoBiologicalInformationResourceswhichincludelecturematerial,video

clippinganddemonstrationandsoon.Theseresourceswouldpromoteunderstandingofadvancedbiologyand

developinteresttothestudents,teachersandpromotequalityhumanresourcetoundertakechallengingresearch

inthe�ieldofmodernbiology.InHimachalPradeshonlyoneInstituteisthere,equippedwiththisBioinformatics

InfrastructureFacility.

Current Biotechnological Projects of key iinstitutions in plant Biotechnological sectors of Himcahal Pradesh

StudiesonAgrobacterium­mediatedgeneticmodi�icationofligninbiosynthesisinHimalayanPoplar(PopulusciliataWall.)forbetterpaperproduction.

Studiesonisolationandcharacterizatioinoftrypsininhibitor(TI)genefromDolichosBi�lorusI.(Kulth)Bioprospectingofthermo­tolerantbacteriafromhotwaterspringsofHimachalPradeshfortheproductionofTaqDNApolymerase

Re�inement for optimization of in vitro rooting and acclimatization of micropropagated plants of clonalrootstocksofstonefruits.

Biotechnologicalinterventionsforestablishmentofownrootedprogenyorchardofsomestonefruits.Germplasmcharacterizationofmodelspeciesandassessmentofcross­speciesSSRstransferabilityinpomeandstonefruitcropsoffamilyRosaceae.

InvitropropagationofSaussureaCostus,anendangeredmedicinalplantofHimalayanregionCreatingagenomicsplatformforappleresearchinIndia.

UnderBTISNET,namely

13

Present status of biotechnology under different sectors in Himachal Pradesh

Improvementofapplethroughtools.Preventing extinction and improvibiotechnologicang conservation status of threatened plants throughapplicationofbiotechnologicaltools.

Developmentofinnovativemethodsforresistanceinductionincucumberagainstfungalfoliardiseases.Biotechnologicalinterventionsforestablishmentofownrootedprogenyorchardofsomestonefruits.Culturable Microbial Diversity of Arnebia euchroma, a critically endangered plant growing in trans­HimalayasofHimachalPradesh.

FunctionalanalysisandvalidationofpicrosidesbiosyntheticpathwayanddevelopmentofgenemarkersforelitechemotypesofPicrorhizakurroa.

Socio Economic upliftment of high altitude farmers of Himachal Pradesh through transfer of micro­propagationtechnologies.

MicrobialinterventionforgeneratingrenewablebioenergyinHPusingpineneedleforest.Transcriptomeanalysisofseabuckthornmaleandfemale�lowers.Moleculardissectionof biosynthesis of pharmacologically importantphenolGlycosides in ahighvaluemedicinalplant(Rhodiolaimbricata)fromTrans­HimalayanRegionofLadakh.

SustainablemanagementoflandthroughimprovedAlbiziaproceraplantsinmidHimalayanregionSelection of PGPR for improvement of leguminous trees for afforestation inNorthWesternHimalayanregion.

Screeningofimportantmedicinalandaromaticherbsforwaterstresstolerance.ExsituconservationofendangeredmedicinalherbsofnorthwesternHimalayas.Developmentofgenemarkersforhighseedoilcontentanddissectingmolecularbasisoffemale�lowerdevelopmentinJ.curcastowardsitsgeneticimprovementforhighseedyield.

DevelopmentofherbalproductforurolithiasisfromTribulusterrestris.Preventingextinctionandimprovingconservationstatusofthethreatenedplantsthroughapplicationofbiotechnologicaltools.

Plantmicrobeinteractioninvolvingvirusandvirus­likepathogensofapple,pigeonpeaandtomato.ComputationalEpigenomics&EpigeneticStudyonTea.Unravelingplant­microbeinteractionsfordiseasemanagement.

14

Present status of biotechnology under different sectors in Himachal Pradesh

GenomicsofMedicinalPlantsandAgronomicallyImportantTraits(PlaGen).PlantBreeding,GenomicsandBiotechnology(PlOMICS)ComparativeevaluationofArnebiaspecieswithspeci�icreferencetometabolomerelatedtoshikoninsynthesis–insitu.

Newinitiativestoboostagricultureproductivitythroughmaximizingpre­andpost­harvestyields(AGROPATHY).

PGPRToolsforImprovingCropProductivityinStressedAgriculturalSystemsBioprospectingMicrobialEndophytesandtheirNaturalProductsfromSomeMedicinalPlantsfromIndianTransHimalayas

Exploitationofresistancegenederivedmarkers(GDMs)forthedevelopmentofblastandbacterialblightresistantversionofricevar.HPR2143.

Developmentofhighyieldingblightresistantchickpea(CicerarietinumL.)usingmutationbreeding.Moleculargeneticsanalysisofresistance/tolerancetodifferentstressinrice,wheat,chickpeaandmustardedincludingsheathblightcomplexgenomics.

MainstreamingFarmer'sVarietiesinHimachalandUttrakhand.DevelopmentofsemidwarfblastandbacterialblightresistantversionofRanbirBasmatibymarkerassistedbackcrossbreeding.

Roleofcellulaseandxylanaseenzymesinbiofuelproductionfromforestwaste.StandardizationofsamplingmethodologyforestimationofareaandproductionofappleinHP.Developmentofyieldforecastingmodel(s)basedonweatherparametersforapplecrop.Puri�icationandcharacterizationofantimicrobialbioactivecompound(s)fromAdiantumcapillus­venerus.Isolation,characterizationandidenti�icationofpesticidetoleratingbacteriafromcontaminatedsoilforbioremediation

Phytophthora,FusariumandRalstoniaofhorticulturaland�ieldcrops(ICARPhytoFuRanetworkproject)EngineeringlateblightresistanceinsusceptibleIndianpotatocultivars(ICAR/ABSPIIcollaboration,USA)

Developmentoftransgenicpotatowithresistancetomajorviruses(ICARnetworkprojectontransgenicsincrops)

NationalCerti�icationSystemfortissuescultureraisedplants(DBT)Symmetricsomatichybridizationforlateblightresistanceinpotato(DBT)Transcriptomepro�ilingofcontrastingpotatogenotypesduringhightemperaturestress(DST)Centreforagriculturalbioinformatics(CABin)(ICAR)CRPofbioforti�ication(ICARplaftorm)Nanotechnology(ICARplaftorm)Exploringthepotentialityofmicro�lorafromHimalayanecosystemforthesynthesisofsilvernanoparticlesandtheirapplicationinmanagementofRalstoniasolanacearuminfectingpotato(DST)Geneticenhancementofpotatothroughmolecularandgenomictools(Institutional)

15

9.2 Animal Biotechnology Sector

9.3 Food and Industrial Biotechnology Sector Current Biotechnological Activities/ Programs and Projects of key institutions in Food and Industrial Biotechnology Sector.

Refinementoftechnologyforproductionoflacticacidfermentedproducts:AsaPostharvestlossreductiontool.StandardizationandCommercializationofAppleCiderVinegarProductionTechnologyfortheSustainableLivelihoodofWeakerSectionsoftheSocietyinHillyAreas.Evaluationofpolymerpackagingmaterialforretainingqualityofsomefruitsandvegetables.Developmentof lowcostvalueaddedprocessedproductsfromripepumpkin(Cucurbitamoschata)anddisseminationoftechnologytothefarmwomenofHimachalPradesh.Researchanddevelopmentofreadytoserveandready­to­cookfoodrecipesforMidDayMealSchemes/ICDS.RefinementofTechnologyforValueAdditionofGingergrowninSolanandSirmourDistrictsofHimachalPradesh.DevelopmentofnovelnutraceuticalsfortifiedwithrarefermentingmicroorganismsisolatedfromHPandtheircommercialpromotion.Bacteriocin:Foodbiopreservative.Value addition of seasonal vegetables/ traditional foods for improved nutritional and livelihoodopportunitiesamonghighlanders.Processesandproductsfromhimalayanregionandtheirtoxicologicalevaluation.Computationalapproachestoimprovethebiophysicochemicalpropertiesofindustrialimportantproteins.Nanoparticle­MediatedTargetedDeliveryofBiomolecules.Nano­materials:ApplicationsandImpactonSafety,HealthandEnvironment.BioprocessdevelopmentforthesynthesisofacetohydroxamicacidusingacyltransferasefromBacillusspMiningofmicrobialgenomesforthenovelsourcesofnitrilemetabolizingenzymesProduction,purificationandbiochemicalcharacterizationofanextracellularcholesteroloxidase fromaBacillussp.CharacterizationofalvularantigenfordiagnosisofRheumaticHeartDisease.Toexploreanti­microbialpotentialoffewimportantmedicinalandaromaticplantsofHimachalPradeshandgreensynthesisofsilvernanoparticlesofplantextractsMicrobial utilization of industrial and other waste for production of commercially viable and usefulproducts

Current Biotechnological Programs and Projects/ Activities/ of key Institutions in Animal Biotechnology Sector of Himachal Pradesh.

All India Co-ordinated

Research Project on Goat

Improvement (Gaddi Field

Unit).

All Indian Co-ordinated

Research Project on Poultry

Breeding (Rural Poultry

Unit).

Studies on the genetic

improvement of mirror carp

and scale carp by selective

breeding (H.P. state).

Empowerment of women

through aquaculture

interventions (DBT).

16

9.4 Biotechnology Education Sector SpecificBiotechnologyeducation(BachelorsandMastersDegree)startedonly fewyearsback inHimachal Pradesh. Prior to this, different branches of biological sciences (Botany, AgricultureScience,andZoology)havecontributedingeneratingBiotechnologyrelatedhumanresourcesinthecountry.CurrentstatusofHR, infrastructureandongoingactivities invariouskey institutionsofBiotechnologyeducationSectorofHimachalPradeshispresentedinthefollowingsection.

Bachelor’s inBiotechnology

H.P.University,ShimlaCollegeofHorticultureandForestry­Neri,HamirpurGovt.PGCollegeMandiHimachalPradeshGovt.PGcollegeDharamshalaHimachalPradeshJaypeeUniversityofInformationTechnologyWaknaghat,Distt.Solan,HimachalPradesh

ShooliniUniversityofBiotechnologyandManagementBajhol,Distt.Solan,HimachalPradesh

Master’s in Biotechnology

H.P.University,Shimla

CollegeofHorticultureandForestry­Neri,Hamirpur

Dr. Y.S. Parmar University of Horticulture & Forestry,

SolanCSKHPKrishiVishavavidyalaya,Palampur

Jaypee University of Information Technology

Waknaghat,Distt.Solan,HimachalPradeshShooliniUniversityofBiotechnologyandManagement

Bajhol,Distt.Solan,HimachalPradesh

Doctorate of Philosophy in

Biotechnology

H.P.University,Shimla

CollegeofHorticultureandForestry­Neri,Hamirpur

Dr. Y.S. Parmar University of Horticulture & Forestry,

SolanCSKHPKrishiVishavavidyalaya,Palampur

Jaypee University of Information Technology

Waknaghat,Distt.Solan,HimachalPradeshShooliniUniversityofBiotechnologyandManagement

Bajhol,Distt.Solan,HimachalPradesh

17

9.5 Forensic Biotechnology Sector

State Forensic Science Laboratory, Junga, Himachal Pradesh, have a covered area of about 28000 square feet. The

work is in progress for planned expansion of additional divisions /specialties in the State FSL.

Biology&SerologyDivision

DNAProfilingDivision

Chemistry&ToxicologyDivision

Narcotics&DrugsPsychotropic

SubstancesDivision

18

10.1 Institute of Himalayan Bioresource Technology, Palampur

ListofPatentsGranted/�iled:

Research achievements in Biotechnology by various Institutes of Himachal Pradesh

Sr.No. Title Inventor

1 Gelprocessingandtransferdevice SanjayKumarandDhirajVyas

2CloningofnovelgenesexpressedistheapicalbudsofCaragana(Caraganajubata)growingundersnow

SanjayKumar,RajeshKumarGuptaandParamvirSinghAhuja

3Cloningofnovelgenesequencesexpressedandrepressedduringwinterdormancyintheapicalbudsoftea(CamelliasinensisL.(O.)Kuntze)bush

SanjayKumar,LakhvirLalandParamvirSinghAhuja

4SpeciesspecificDNAsequencesandtheirutilizationinidentificationofviolaspeciesandauthenticationof"banafsha"bypolymerasechainreaction

MahipalSingh,ChandanSharmaandBrijLal

5Auniversalplasmidvectorfortransformingchloroplastsofhigherplants

MahipalSinghandBandanaDhiman

6SpeciesspecificgenomicDNAsequenceforidentificationAnacardiumoccidantaleandthemethodforitsutilizationindetectionofcashewhuskinmadeteasamples

MahipalSinghandBandanaDhiman

7Aconvenientandversatile,compactportablelightweight,lowpowerconsumingsterilelaminarair�lowsystem

RajeshThakur,AnilSoodandParamvirSinghAhuja

Numberofmajorprojectsonbiotechnology:27

Totalnumberofstudentsenrolledinbiotechnology:59

Totalnumberofpublications:61

Numberofpatentsgranted/�iled:43

19

9Anefficientmethodformicropropagationoftea(Camelliasinensis)plantsusingleafexplants

IndraSandal,AmitaBhattacharya,MadhuSharmaandParamvirSinghAhuja

10Anefficientonestepmethodformicro­productionoftealeaves

IndraSandal,AmitaBhattacharya,MadhuSharmaandParamvirSinghAhuja

11AmethodforinducingimprovedseedgerminationinahighaltitudemedicinalandendangeredplantPodophyllumhexandrumRoyle

AmitChandraKharkwal,AmitaBhattacharya,OmPrakash,PramodKumarNagarandParamvirSinghAhuja

12Anovelisozymeofautoclavablesuperoxidedismutage(SOD)­aprocessfortheidentificationandextractionoftheSODanduseoftheSODincosmetic,foodandharmaceuticalcompositions

SanjayKumar,RashmitaSahooandParamvirSinghAhuja

13 AnefficientnewmethodforprotoplastculturePratapKumarPati,MadhuSharmaandParamvirSinghAhuja

14Cloningof3novelgenesmodulatedunderdroughtstressconditionsintea(CamelliasinensisL.(O.)Kuntze)

PreetiSharma,SanjayKumar,ParamvirSinghAhuja

15CaffeinefractionobtainedfromtealeavesandamethodforinducingAgrobacteriumtumefaciens­mediatedgenetictransformationinplantsusingsaidcaffeinefraction

IndraSandal,AjayKumar,AmitaBhattacharya,SrigurupuramDesikacharyaRavindranath,AshuGulati,ParamvirSinghAhuja

16Amethodforbiolisticmediatedgeneticransformationoftea(CamelliasinensisL.(O.)Kuntze)usingleafexplants

IndraSandal,AmitaBhattacharya,ParamvirSinghAhuja

17 AnimprovedmethodofteapropagationOmPrakash,AnilSood,MadhuSharmaandParamvirSinghAhuja

18 AnimprovedacclimatizationunitAnilSood,MadhuSharmaandParamvirSinghAhuja

19Novelgenesfromdroughtstresstolerantteaplantamethod(CamelliaSinensis(L)O.Kuntz)

SharmaP,KumarS,AhujaPS

20UseoftealeafextractasapotentbactericidalagentforAgrobacteriumtumefaciennsmediatedgenetictransformations

IndraSandal,AmitaBhattacharya,AshuGulati,SrigurupuramDesikacharyaRavindranath,ParamvirSinghAhuja

21 TransgenicteathroughbiolisticusingleafexplantsIndraSandal,AmitaBhattacharya,ParamvirSinghAhuja

22Useoftealeafextractforfieldinhibitingmicrobialtransformant

IndraSandal,AmitaBhattacharya,AshuGulati,SrigurupuramDesikacharyaRavindranath,ParamvirSinghAhuja

23DevelopmentofdiagnostickitagainsttherecombinantcoatproteingeneforthedetectionofCarnationMottlevirus

GauravRaikhy, VipinHallan, Saurabh Kulshreshtha, AA Zaidi

8AnimprovedmethodformasspropagationofPodophyllumhexandrumRoyleusingembryoculturetechnique

AmitChandraKharkwal,AmitaBhattacharya,OmPrakashandParamvirSinghAhuja

Sr.No. Title Inventor

24DevelopmentofdiagnostickitforthedetectionofchrysanthemumvirusB

LakhmirSingh,VipinHallan,AAZaidi

25Developmentofdiagnostickitagainsttherecombinentcoatproteinofprunusnecroticringspotvirus

SaurabhKulshreshtha,VipinHallan,GauravRaikhy,AAZaidi

20

28Acosteffective,lesshazardous,two­solutionsystemforrapidisolationofRNA

GhwanaSanjay,SinghKashmir,RaizadaJ,ArtiRani,BhardwajPradeepKumar,KumarSanjay

29Superoxidedismutase(SOD)CDNAfromPotentillaatrosanguineaanditsexpressioninhetrologoussystem

BhardwajPK,SahooRashmita,KumarS,AhujaPS

30AcompositionandmethodforremovalofcoloursandinhibitorsfromtissuestoisolateRNA

RaviShankerSinghandSanjayKumar

31Amethodforcloningfunctionalgeneofcopper/zincsuperoxidedismutasesusingoligonucleotideprimers

BhardwajPardeep,KumarArun,KishoreAmit,GhawanaSanjay,RaniArti,SinghKashmir,SinghHarsharan,RaviShankar,KumarHitesh,SoodPayal,DuttSom,KumarSanjay,AhujaParamvir

32 Auniversalfungalpathogendetectionsystem KarnikaThakur,GopaljeeJha

33Superoxidedismutase(SOD)geneandamethodofidentifyingandcloningthereof

PardeepKumarBhardwaj,RashmitaSahoo,SanjayKumar,ParamvirSinghAhuja

34 Anexpressionconstructandprocessforenhancingthecarbon,nitrogen,biomassandyieldofplants

AnishKaachra,SKVats,PSAhujaandSanjayKumar

35 AnautoclavestablerecombinantCu/Znsuperoxidedismutasewithenhancedthermo�lexibility

ArunKumar,SomDutt,PSAhuja,SanjayKumar

36Methodandapparatusfortheseparationofseedsfromfruitpulp/slurry/pomace

ShashiBhushan,SakshiGupta,GarikapatiDivyaKiranBabu,MohitSharma,ParamvirSinghAhuja

37 Abioreactorvesselforlargescalegrowingofplantsunderasepticconditions

RajeshThakur,AnilSood,ParamvirSinghAhuja.

38Anovelhybridnanocompositematerialforoptical/mribimodalmolecularimagingandaprocessforthepreparationthereof

AmitabhaAcharya

39AnovelembryosacspecificbidirectionalpromoterfromArabidopsis

SreenivasuluY,SharmaIsha,AhujPS,SrinivasanRandBhatRamchandraSripad

40Novelbidirectionalpromotertrapconstructtotrap/identifynovelpromoters

YelamSreenivasulu,RimpyDiman,RamamurthySrinivasan,ShripadRamachandraBhat,ParamvirSinghAhuja

41AnovelmediumcompositionforculturingtheentomopathogenicfungusLecanicilliumlecanii

SGEReddyandShaliniSahotra

42Ananobiocompositeforwoundhealingandaprocessforthepreparationthereof

SudeshKumarYadav,RubbelSingla,AvneshKumari,

43Aninnovativeprocessfordevelopmentofreadytoeatcrispyfruitswithhighquality

MaheshGupta,JaiPrakashDwivediandSanjayKumar

Sr.No. Title Inventor

26Useof2­Aminoglutarimideinpromotingagrobacteriumtumefaciene

SandalI,BhattacharyaA,RavindranathSD,SinghB,KaulVK,SainiU,KumarJK,AhujaPS,GuptaAP

27Agreenprocessforthepreparationofsubstitutedcinnamicesterswithtrans­selectivity

SinhaAK,SharmaA,SwaroopA,KumarV

21

DevelopedmicropropagationprotocolsforendangeredandcriticallyendangeredmedicinalplantsofwesternHimalaya.

Generatedplantingmaterialsofsaffron DevelopedPGPRsforimprovedcropgrowthandyield. Identi�iedseveralmicrobesassourcesoftherapeuticenzymes. ThemechanismofviralinfectionofappleandValerianajatamansideciphered. Developedmultiplexdiagnosticsforappleandcherryvirusandvirus­likepathogens. Identi�ieddormancy­relatedanddiseaseresistancegenesthatwillleadtoimprovementofphenologicaltraits

inapple. Developing �luorescentnanomaterials as cellular imagingprobe andalsonanomaterials fordeliveryof

biomolecules Identi�iedplant­derivedmoleculesfromHimalayasasanti­oralcanceragents.

Latest achievement in the field of Biotechnology:

Products:Steri�low,GelProtrad,IrisSolution,Viraldiagnostickits,ThermostableautoclavableSOD,Rootingvesselfortissuecultureraisedplants,Bioinformaticsregulomicsserver, Tissuecultureraisedplantsofhighvalue and quality (tea, bamboo, scented rose, lavender, coleus, saffron, endangeredmedicinal plants etc.,PGPRs,therapeuticenzymes),geneandgeneticresourcesofhighvalueplants(tea,bamboo,Picrorhizakurroaetc.),constructswithnovelgenesfromHimalayanbioresource,transgenicplantsofapplerootstock.

Micropropagationprotocolsfortea,bamboos,scentedrose,lavender,aloevera,endangeredplantsofhigherreachesofHimalayas,virustestedapplerootstocks.

Tissueculturemethodforeliminationofviruses Genetictransformationofwoodyplantsandmodelplants PCRbasedmethodsforviraldetection Identi�icationandproductionofPGPRsforimprovedcropgrowthandyield Bioinformatictoolsforearlydetectionofhumandiseases. Processforsynthesisofbioactiveloadednano­particles. Applepomacefordietary�ibres.

Department Products /Process Development and Transferred/to be transferred:

22

10.2 Central Potato Research Institute, Shimla-171001 (HP)

Analysisoffullgenomesequenceofpotatodryrotpathogen(Fusariumsambucinum).

AnalysisoffullgenomesequenceofpotatobacterialwiltpathogenRalstoniasolanacearum.

Mapping of completemitochondrial genome of potato late blight pathogen (Phytophthora infestans A2

matingtype)

SilencingofPhytophthorainfestansmulti­genesinpotatousingsingleRNAiconstruct.

StabilityandfunctionalityofRBgeneinlateblightresistanttransgenicpotato.

RNA­sequencingofwildpotatospeciesSolanumdemissumforlateblightresistance.

Geneexpressionanalysisofcyclicdependentgeneforpotatotuberization.

Identi�icationofPALCV(potatoapicalleafcurlvirus)resistancegenesinpotato.

Moleculardiversityinthepotatoassociationmappingpanel.

Molecular characterizations of interspeci�ic somatic hybrids (Solanum tuberosum dihaploid C­13 + S.

cardiophyllum)usingAFLPmarkersandtheirlateblightresistanceassay.

Breeding potential of potato somatic hybrids under the sub­tropical plains of India and evaluation of

advancedprogeniesofF1C3population.

Molecularpro�ilingofsegregatingprogeniesofsomatichybridsdevelopedbycrossingwithpotatovarieties.

Enhancingnitrogenuseef�iciency(NUE)inpotatocropthroughgenomicsapproaches.

PlantgrowthunderaeroponicssystemtostudyNUE

Transcriptomesequencing(RNA­seq)forNUEandsequencevariationsforN­metabolismgenesinpotato.

Sequenceanalysesoflateblightresistancehomologousgenesisolatedfromwildpotatospecies.

Latest achievements in the field of Biotechnology

Effectiveexploitationofgeneticresourcesforvarietalimprovement

Safeapplicationofbiotechnologyforpotatoimprovement

Encouragingproductionofqualityplanting

material Resourcebasedplanningandcropmanagement Effectiveandeco­friendlycropprotection Encouragingenergyef�icientstorageanddiversi�iedutilizationofpotato

RoleofGovernmentinpromotionofbiotechnologyinHimachalPradesh(StrategyandFrameworkoftheinstitute;fromCPRIVision2030)

Numberofmajorprojectsonbiotechnology:12

Totalnumberofstudentsenrolledinbiotechnology:28

Totalnumberofpatentsgranted/publications:31

Toexecutestructuralandfunctional

genomicsforpotato

improvementapplying

biotechnologicalintervention(2016­17).

23

10.3 CSK HimachalPradesh Krishi Vishvavidyalaya, Palampur

NumberofmajorprojectsonBiotechnology:8

TotalnumberofstudentsenrolledinBiotechnology:15

TotalnumberofPublications:57

Numberofpatentsgranted/�iled:2

List of Patents granted/filed:

Sr. No. Title Inventor

1 MelaninproductioninAscochytarabieiisinvolvedinpathogenicityonchickpea

WChen,KDSharma,MWheelerandFMuehlbauer

2 TheroleofmelaninproductioninAscochytablightofchickpea

WChen,KDSharma,MHWheelerandFJMuehlbauer

DevelopmentofgenomicresourcesinHorsegram.

WholegenomesequencingofHorsegramvarietyHPK4.

Powderymildewresistantpealinesdevelopedbyintegratingresistancegenes'er1'and'er2'intothe

geneticbackgroundofsusceptiblecommercialvarieties“Lincoln”andAzadPea­1throughmarker­

assistedbackcrossbreeding(MAB).

Identi�icationandmappingof�ivenewblastresistancegenesPi­42(t),Pi­w(t),Pik­landPi­tpandPibl2.

Themarkerslinkedtothesegenescanbeexploitedinmarkerassistedbreedingprogramsfor

developmentofblastresistantricevarieties.

Nearisogeniclines(NILs)andpyramidlinesofamegaricevarietySambhaMahsuriharbouringdifferent

combinationsofblastresistancegenesPi­ta,Pi­1andPi­kh.

Latest achievement in the field of Biotechnology:

PowderymildewresistantversionsofcommercialPeavarietiesLincolnandAzadPea­1

BacterialblightandblastresistantversionsofricevarietyHPR2143.

Department Products /Process Development and Transferred/to be transferred:

24

NumberofmajorprojectsonBiotechnology:10

TotalnumberofstudentsenrolledinBiotechnology:140

TotalnumberofPublications:26

Numberofpatentsgranted/�iled:8

10.4 Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni (Solan)

Sr.No. Title Inventor

1 AprocessforinvitrouseofAsparagusspeciesextractsfor

carnationlatentviruselimination.IndianPatentNo.192157.BhardwajSV,MangalManishaandAnilHanda.

2Aprocessforin­vitroeliminationofplantvirusbypreparingandusinganextractofAzadirachtaindicaplant.IndianPatentNo.193932.

BhardwajSV,Shalini,AnilHandaandManisha

Mangal

3 Aprocessforpreparationofabio­reagentforusefor

eliminationofplantvirus.IndianPatentNo.199706.

BhardwajSV,SJRoy,ManishaMangalandAnil

Handa

4AnextractoffruitsandrootsofAsparagusadscendensor

Asparagusofficinalisusefulfortheeliminationofcarnation

latentvirusfrominfectedplantsbysupplementingthetissue

culturemedium.IndianPatentNo.232448.

BhardwajSV,ManishaMangalandAnilHanda

5Celllinesofchrysanthemum(Dendranthemagrandiflorum

Tzelev)cv.SnowBallresistanttoSeptoriaobesaSyd.Indian

PatentapplicationNo.1334/DEL/2007filedon21.05.2007.

KumarS,S.KumarandS.V.Bhardwaj

6DevelopmentofreproducibleprotocolfordirectorganogenesisintransgenicmaleplantofHimalyanpoplar.PatentApplicationNo.1591/DEL/2007dated27.7.2007.

Thakur,A.K.,ShandilR.K.andSrivastavaD.K

7AnextractofcormofStephaniaglabrausefulforthe

eliminationofcucumbermosaic(CMV)andBeanYellow

Mosaic(BYMV)virusesfromgladiolusbysupplementingin

tissueculturemedium.FiledthroughNRDCNo.IPR/4.19.1/10053

BhardwajS.V,BhattaraiA,HandaA&ParmarN.

8

Molecularmarkerslinkedtothegeneexhibitingresistance

againstblackrot(Xanthomonascampestrispv.campestris

(Pammel)Dawson)incauliflower(Brassicaoleraceavar.

botrytis).FiledthroughNRDCNo.IPR/4.18.13/10020.

KaurR,KashyapM,SaxenaB,KanwarHS,

DohrooNPandBhardwajSV

25

Latest achievements in the field of Biotechnology

Shootregenerationprotocolfortomatocv.SolanLaimaTransferofInvitropropagatedplantsofPlum­SantaRosaandFrontierto�ieldInvitromutagenesisingingerwithgammaradiationsforselectionofFusariumresistanceDevelopmentofsomaclonalvariantsinappletoleranttofungusPhytophthoracactorumMicropropagationofcrabapple(Malusbaccata)biotypesMicropropagationofS.costus(Kuth)­endangeredmedicinalplantofHimalayanregion

1) Plant Tissue Culture

2) Plant Molecular Biology [Genetic Transformation]Studies on Agrobacterium­mediated insect resistance gene [cry1A(a)] transfer in cauli�lower (BrassicaoleraceaL.var.botrytis)­

StudiesonAgrobacterium­mediatedinsectresistancegene[cryIA(a)]transferinbroccoli(BrassicaoleraceaL.var.italica)andmolecularanalysisofregeneratedtransgenicplantlets­

3) Cloning and transformationRestrictiondigestionofplantexpressionvector(pBI121)Integration of trypsin inhibitor gene into plant expression vector and transformation of E. coli top 10competentcells

Con�irmationoftransformedbacterialcolonyRestrictiondigestionofrecombinantplasmidExpressionoftrypsininhibitorgeneinE.colicellsIntegrationoftrypsininhibitorgeneintopJET1.2/bluntcloningvectorCon�irmationoftransformedbacterialcoloniesIsolationofrecombinantplasmidfromtransformedbacterialcoloniesRestrictiondigestionofrecombinantplasmidsRestrictiondigestionofpET23a(+)heterologousvectorIntegrationoftrypsininhibitorgeneintoheterologousexpressionvector(pET23a)andtransformationofE.coliBL21competentcells

Con�irmationoftransformedbacterialcoloniesRestrictiondigestionofrecombinantplasmidSequencingofrecombinantpJET1.2/bluntcloningvector

Biotechnology products/processes developed and transferred / to be transferred

Themultipleshootculturesandrepeatablemicropropagationprotocolsofsomepromising,dwarfandsemidwarfapplerootstockslike,MM106,MM111,M7,M26andMerton793developed,re�inedforlargescaleplantproductionand�ieldtransfer.ThesehavebeensuccessfullyusedatPilotscalebyTERI(TheEnergyandResourcesInstitute),NewDelhiundera“NetworkProgrammeonApple”,fundedbytheDepartmentofBiotechnology,GovernmentofIndia,NewDelhi,in2003­2008.

MultipleshootculturesoftheseapplerootstockssoldtolocalentrepreneursengagedinproducinglargenumberofappleplantsintheirtissuecultureunitsestablishedinvariouspartsofHP.

MicropropagationprotocolsofEMLA9andindigenousrootstockcrabappledevelopedfortransfer.Protocolforinvitromultiplicationforstrawberrycv.OfrahhasbeenstandardizedandhandedovertofarmerofRajgarh(HP).

Bioinformatics centre: i) Developed �ive video CDs onTemperateHorticulture ii) Developed atrainingmanualon'ComputerBasics'.

Varioususeragencies/TissueCultureunitsforcommercialproductionoftissueculturedplantsweresetupinHPunderNationalHorticultureTechnologymission.

Role of Government in promotion of Biotechnology in Himachal Pradesh

26

10.5 Himachal Pradesh University,Shimla.

NumberofmajorprojectsonBiotechnology:6

TotalnumberofstudentsenrolledinBiotechnology:61

TotalnumberofPublications:85

Numberofpatentsgranted/�iled:5

Production of various important acids such as nicotinic acid, benzohydroxamic acid, acrylic acid,nicotinahydroxamicacid,2­hydroxybenzoicacid,picolinicacid,acetohydroxamicacid,butyrohrdroxamicacid,Taxol,propylgallate,6­aminopenicillanicacidandindustriallyimportantacrylamide,nano­particlesofZn,Fe,Agetc.withandwithoutenzymeloadingandantimicrobicidesofFernorigin.

Besidesvariousproductionofimportantorganicacids,shortandlongchainesterswithanti­oxidant/freeradical scavenging activities/ fragnance compounds etc., the department focuses on the production ofbiofuels,healthcareandherbalprocessing.

Biotechnological products

Sr.No Title Inventor

1 Formulationofantibacterialfernmouthwashsolutionfororalhygine

Prof.S.S.Kanwar,DepartmentofBiotechnology.

2Hyperproductionofananti­cancerdrugTaxolfromendophyticAspergillusfumigatusisolatedfromTaxussp.ofHimachalPradesh

Prof.DuniChand,DepartmentofBiotechnology

3 ProcessdevelopmentforwheatflourtreatmentusingphytasefromAspergillusfumigatus.

Prof.DuniChand,DepartmentofBiotechnology

4 ANano­BioconjugatefortheSynthesisofNovelAnaestheticDrugBenzocaine

AbhishekSharmaandShamsherSinghKanwar

5Screening,IsolationandPurificationofL­methionaseEnzymefromKlebsiellaoxytoca

BhupenderSharmaandShamsherSinghKanwar

27

10.6 Jaypee University of Information Technology, Waknaghat, Solan (HP)

NumberofmajorprojectsonBiotechnology:21

TotalnumberofstudentsenrolledinBiotechnology:291

TotalnumberofPublications:195

Numberofpatentsgranted/�iled:16

Sr. Title Inventor

1HennaBasedDye,ProcessforPreparingtheSameandUsethereofinSDS­PAGEMethod

TandonC,ChaudharyA,GuptaSandJainS

2 Non­NaturalShortCationicAntimicrobialLipopeptides BishtGSandLohanS

3AutomatedComputerAidedDiagnosis (CAD)of TuberculosisusingChestX­Ray

RoutC,ChauhanA,ChauhanRSandChauhanD

4AprocessofenrichingmedicinalconstituentsinshootculturesofSwertiachirayita

SoodH,GulatiS,BakshiS,SandhuBS,KumarVandChauhanRS

5 INDITECH­ANovelBioreactor SharmaS,SoodHandChauhanRS

6Asynbioticformulationofseabuckthorn(HippophaerhamnoidesL.)berrieswithradicalscavengingpotential

DeyGandNegiB

7 Isolationandpuri�icationofPicroside­IandPicroside­IISoodH,KumarVandChauhanRS

8AneconomicalandimprovedisolationprocessofantitumortriterpenoidLantadeneBfromweedLantanacamaraL.

SharmaMandKumarSS

9AnimprovedprocessofconversionofLantadeneA&BtoreducedLantadeneA&B

SharmaMandKumarSS

10Processforproductionofcellulaseandpectinasefromherbalindustryresidue

KumariM,KumarS,ChauhanRSandRavikanthK

11 Anovelanti­calcifyingproteinfromTribulusterrestris TandonCandAggarwalA

12Thermo­halotolarentLipasebyaNewStrainofStaphylococcusarlettae

ChauhanRS,AnwarT,MathurA,MalhotraAandMathurG

13 Novelmethodforproducingseabuckthornwineandwinethereof DeyGandNegiB

14GeneMarkersforSelectionandDevelopmentofHighOilContentJatropha

ChauhanRS,SharmaAandSoodP

15Aprocessofenrichingtheamountofmedicinalcompound,Picroside­1inshootsofmedicinalherb,Picrorhizakurroa

SoodHandChauhanRS

16BiosyntheticpathwayfortheproductionofPicroside­IandPicroside­IIandtheirIntermediatesinPicrorhizakurroa

ChauhanRS,KumarV,SoodH,PanditSandShitizK

28

TheDepartmentofBiotechnology&Bioinformaticshasbeenranked1stamongall

privateuniversitiesandinstitutionsinIndiaforthelastfouryears.In 2013, the Association of Biotech Lead Enterprises (ABLE) has honored the

institution with one of the best private biotech and bioinformatics education

institutionsinthecountryfordoingoutstandingresearchandeducationduetoits

strong R&D program, up­to­date educational curricula, modern laboratory

infrastructure,highlyquali�iedfaculty,IPR&patents,andplacementsofgraduates.

Latest achievement in the field of Biotechnology:

Environmentalfriendlydyeforstaining(patentgranted).

Biogas­ Biogas Digesters (08 in number) Installed at different places in HP. E.g.

Technology transferred toAyurvet Industry,EternalUniversity,Govt. Schools,HP

StateScienceCouncil,Shimla.(2008­2017)Vermicompost­Technology transfer to Ayurvet Industry,Baddi, Solan,Himachal

Pradesh(2010).Antimicrobials: Synthesis of small molecules and peptidomimetics as lead

antimicrobialcompounds.Bioreactor: Development of a novel bioreactor for plant tissue culture (patent

applied).DiagnosticofPathogens­CharacterizationofdiarrhoeagenicpathogensinHimachal

PradeshIndustrialEnzymes­Xylanases&Cellulasesforapplicationinpaperpulpandbiofuels

industriesElectronicwasteresourcerecovery–Metalleachingtechnologytobetransferredto

formale­wasterecyclingindustries

Department Products /Process Development and Transferred/to be transferred:

29

10.7 Directorate of Mushroom Research, Solan - 173212

TwononBrowningstrainsandonehighyieldingstrainofwhitebuttonmushroomweredeveloped(NBS­1,NBS­5andU3­54)andreleasedforcommercialuse.

Latest achievements in the field of Biotechnology

Strategicandappliedresearchoncollection,conversation,utilizationandproductionofedibleandmedicinalmushroom.

Priority area of inter-institutional Co-Operation and coordination

VarietydevelopedNBS­1,NBS­5andU3­54.

Biotechnology products /process developed and transferred / to be transferred

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31

NameofExpert Designation Institution

Dr.SudeshKumar PrincipalScientist IHBT­Palampur,[HP]

Dr.RamKumarSharma PrincipalScientist IHBT­Palampur,[HP]

Dr.Y.Sreenivasulu PrincipalScientist IHBT­Palampur,[HP]

Dr.RiturajPurohit SeniorScientist IHBT­Palampur,[HP]

Dr.RaviShankar Scientist IHBT­Palampur,[HP]

Dr.YogendraS.Padwad Scientist IHBT­Palampur,[HP]

Dr.AmitabhaAcharya Scientist IHBT­Palampur,[HP]

Dr.VishalAcharya Scientist IHBT­Palampur,[HP]

Dr.KunalSingh Scientist IHBT­Palampur,[HP]

Dr.AjayRana Scientist IHBT­Palampur,[HP]

Dr.RajivKumar Scientist IHBT­Palampur,[HP]

Dr.AshishRambhauWarghat Scientist IHBT­Palampur,[HP]

Dr.MaheshGupta Scientist IHBT­Palampur,[HP]

Dr.DharamSingh SeniorScientist IHBT­Palampur,[HP]

Dr.ShashiBhushan SeniorScientist IHBT­Palampur,[HP]

Dr.AmitaBhattacharya ScientistE­II IHBT­Palampur,[HP]

NameofExpert Designation Institution

Prof.T.C.Bhalla ScientistEmeritus HPUniversity­Shimla,[HP]

Dr.ArvindKumarBhatt Professor HPUniversity­Shimla,[HP]

Dr.(Ms)ReenaGupta Professor HPUniversity­Shimla,[HP]

Dr.S.S.Kanwar Professor HPUniversity­Shimla,[HP]

Dr.DuniChand Professor HPUniversity­Shimla,[HP]

Dr.WamikAzmi Professor HPUniversity­Shimla,[HP]

Dr.Savitri Asstt.Professor HPUniversity­Shimla,[HP]

Inst

itu

te o

f Him

ala

yan

Bio

reso

urc

e T

ech

no

log

y P

ala

mp

ur,

Him

ach

al P

rad

esh

.H

ima

cha

l Pra

de

sh- U

niv

ers

ity,

Sh

imla

Experts of Biotechnology in Himachal Pradesh from various Institutes

32

NameofExpert Designation Institution

Dr.SKChakrabarti Director&PrincipalScientist CPRI­Shimla,[HP]

Dr.JageshKumarTiwari Scientist(SeniorScale) CPRI­Shimla,[HP]

Mr.HemantKardile Scientist,Biotechnology CPRI­Shimla,[HP]

Mr.VUPatil Scientist,Biotechnology CPRI­Shimla,[HP]

Dr.VinayBhardwaj PrincipalScientist, CPRI­Shimla,[HP]

Dr.SundareshaS Scientist(SeniorScale), CPRI­Shimla,[HP]

Dr.ShashiRawat Sr.Scientist CPRI­Shimla,[HP]

Dr.SomDutt Sr.Scientist CPRI­Shimla,[HP]

Dr.GVanishree Scientist CPRI­Shimla,[HP]

Dr.BaswarajR Scientist(SeniorScale) CPRI­Shimla,[HP]

Dr.RavinderKumar Scientist CPRI­Shimla,[HP]

Dr.VinaySagar SeniorScientist CPRI­Shimla,[HP]

Dr.SanjeevSharma SeniorScientist CPRI­Shimla,[HP]

Dr.BrajeshSingh PrincipalScientist&Head CPRI­Shimla,[HP]

Dr.AartiBairwa Scientist CPRI­Shimla,[HP]

Dr.TanujaBuckscth Scientist CPRI­Shimla,[HP]

Dr.RKSingh Head&PS CPRI­Shimla,[HP]

Dr.SalejSood Scientist CPRI­Shimla,[HP]

Ce

ntr

al P

ota

to R

ese

arc

h In

stit

ute

, Sh

imla

( H

ima

cha

l Pra

de

sh )

NameofExpert Designation Institution

Dr.(Mrs.)AmarjitK.Nath Professor&Head UHFNauni­Solan,(HP)

Dr.(Mrs.)NiveditaSharma Professor UHFNauni­Solan,(HP)

Dr.(Mrs.)PoonamShirkot Professor UHFNauni­Solan,(HP)

Dr.(Mrs.)ManjuModgil Professor UHFNauni­Solan,(HP)

Dr.(Mrs.)Sunita AssistantProfessor UHFNauni­Solan,(HP)

Dr.(Mrs.)RajinderKaur Professor UHFNauni­Solan,(HP)

Dr.RajnishSharma AssistantProfessor UHFNauni­Solan,(HP)

Dr.(Ms.)ManishaThakur AssistantProfessor UHFNauni­Solan,(HP)

Dr.(Ms.)SnehLataSharma AssistantProfessor UHFNauni­Solan,(HP)

Dr.

Y S

Pa

rma

r U

HF,

Na

un

i , S

ola

n (

H.P

)

33

NameofExpert Designation Institution

Dr.O.PAlahawat PrincipalScientist ICAR­DMR

Dr.AnupamBarh Scientist ICAR­DMR,Solan

Dir

ecto

rate

of M

ushr

oom

Experts of Biotechnology in Himachal Pradesh from various Institutes

Statehasanurgentneedtoincreaseagriculturebasedproductstomeetthegrowingdemandsundertheconstraintsof

depletingnaturalresourcesandenvironmental�luctuationsinthestate.Yearafteryearproblemsarebecomingmoreintensetherebyhighlightingtheneedtofocusresearchonareaslike

temperaturetolerance,diseaseresistanceandqualityproductionofgrain,foodandhorticulturalproducts,etc.Despiteintensiveworkbyscientistsongenerationofendangeredmedicinalplantsofparticularly,higherreachesof

Himalayas,theplantscontinuetosufferfromindiscriminateuprootingandillegaltrading.Activeresearchintheeducationalinstitutethatinvolvesapproaches,suchas,plantbiotechnologybasedapproaches

and stress biology, healthcarebiology, identi�icationof responsive genes/alleles, followedbymutant analysis ortransgenicapproachestoassignthefunctionofspeci�icgeneoritsproductproteinisthenewtooltodealwiththisissue.Developmentofheattolerantgenotypesinthebiotechnologyresearchlabisthecheapestmeansofcombatingtheheat

and high temperature stress. New lines should be developed having high yielding attribute in addition to theiradaptabilitytowarmerclimates.Qualityseedproduction.

Herbicideshaveamajordisadvantageofkillingthemainplantvarietiesalongwiththeweeds,whichresult into

greaterloss,whichisdirectlyorindirectlyaffectthefarmer.Herbicidetolerantvarietiesprovidenewoptionsforthecontrolofmajorweeds,whichareconstrainingagriculturalproduction,Herbicidetolerantveritycanbedevelopedinthestatebypromotingtheresearchinbiotechresearchlaboratory.Earlydiagnosisof infectiousdiseasesandeffectivecontrolofpathogensandsafedisposalofwastebymicrobial

interventions.Majorhealthcareissueinthestateisrelatedwithlackofawareness;majorityofpatientseeksconsultationonly,when

thediseaseisalreadyatanadvancestage.Toovercometheabovechallenges,thereisanurgentneedofresearchtodevelop cost effective biomedical devices (based on tissue engineering or nano biotechnology or animalbiotechnology)forearlydiagnosisandtreatmentofdiseaseintheruralarea.Blendofinnovativeresearchapproachescanimprovethedrowningconditionsinthefuture.

There is no system in place to take the biotechnology products to the industry due to absence of linkages and

partnershipdevelopment.LimitedopportunitiesinBiotechnologyintheState.

Genetictransformationforbiotic/abioticstresstolerance

Cloningofgenesfromvariousorganisms

Geneticallyengineeredmicroorganisms

Issues and Challenges:The issues and challenges suggested by various R&D Institutes in the state is listed below so that action related to address these issues

and challenges are initiated to promote Biotechnology in the larger public interest.

NameofExpert Designation Institution

Dr.T.R.Sharma Professor&Head CSKHPKV­Palampur,[HP]

Dr.K.D.Sharma Professor CSKHPKV­Palampur,[HP]

Dr.R.K.Kapila AssociateProfessor CSKHPKV­Palampur,[HP]

Dr.R.Rathour AssistantScientist CSKHPKV­Palampur,[HP]

Dr.YPThakur Professor CSKHPKV­Palampur,[HP]

Dr.VarunSankhyan(AnimalGenetics) AssistantProfessor CSKHPKV­Palampur,[HP]

Dr.SubhashVerma AssociateProfessor CSKHPKV­Palampur,[HP]

CSK

HPK

V- P

alam

pur,

Him

acha

l Pra

desh

34

Department of Environment, Science and TechnologyGovt. of Himachal Pradesh

“Attaining new heights in biotechnology research, shaping biotechnology into a premier

precision tool of the future for creation of wealth and ensuring social justice-specially for the

welfare of the poor”

ParyavaranBhawan,U.S.Club,Shimla­171002,HimachalPrdeshPhoneNo.:0177­2655456,2656559fax:0177­2659609

Email:dbt­hp@nic.in

HIMACHALPRADESHSHIMLA­171002

35