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Photonic Biosensors
Faculty AdvisorDr. Seung Kim
© Israel Flores 2016
Importance
• Hundreds of thousands of deaths from treatable diseases in each year• Early detection increases survival rates
© Israel Flores 2016
Our Goal
• Early diagnosis the key to effectively
treating diseases like cancer.
• Low Cost
• Point of Care Diagnosis
• Portable Biosensor
To develop a Point-of-Care (POC) Biosensor for medical diagnosis to monitor health status
© Israel Flores 2016
CD Based Microfluidics
Laura DeOtteLuke Funk
© Israel Flores 2016
Why CD-based Microfluidics?•Optical system requires fluid to be in a
~2.5 µm cavity
•Microfluidics effectively transports fluid from input to sensing region
• CD-based design: • Space effective
• Cost effective
• Controllable fluid flow
© Israel Flores 2016
Microfabrication: Photolithography
• Silver sputtering
• SU8 spin deposition
• UV light exposure
• SU8 developing
• Bonding
© Israel Flores 2016
Fall 2015 Design
© Israel Flores 2016
CD Mask Design
© Israel Flores 2016
Channel Selection
• Fluid ‘split’ in channels with a large change in cross-section
• Fluid continuous in uniform channels • Easier to fabricate • 1 mm channel width
© Israel Flores 2016
Results
• Capillary action capable of filling channel and sensing region
• Reduce fill time by spinning
© Israel Flores 2016
Results• Spinning necessary to transport fluid
to waste reservoir
• Goals: • Use calibration fluid • Reusable channels
© Israel Flores 2016
Future Plan• Disc design improvements
• Simplify microfabrication
• Easier to align sensing region
• 4 identical channels on each disk
• Functionalization of sensing region surface with bioreceptors
© Israel Flores 2016
Optical System
Tony Bujana
© Israel Flores 2016
Optical System
© Israel Flores 2016
Fabry-Perot Cavity
R: reflectance of partially reflecting mirrorL: distance between the mirrorsn: refractive index inside of the cavity
© Israel Flores 2016
Scaled Differential Calculation
ΔP1= -0.5262ΔP2= 0.39 Δη= 1.252
© Israel Flores 2016
ResultsCamera 1. 780nm Laser
Camera 2. 850nm Laser
© Israel Flores 2016
Future Plan (Bio-detection)
© Israel Flores 2016
Stopping Mechanism
Nathan Strachen
© Israel Flores 2016
Control System
• Precise positioning of disk• Closed loop control • User friendly
Rotate CD at high RPM for a specified
period of time Stop CD at a specific angle
© Israel Flores 2016
Our Approach
• Dual-Motor system - Stepper Motor - DC Motor - Pulley Connection
• Stepper motor allows for precise positioning • DC Motor enables rotation speeds up to
4600 RPM• IR sensor is used for reference point
© Israel Flores 2016
Implementation & Results
© Israel Flores 2016
Enclosure System
Stephen Sumrall
© Israel Flores 2016
Photonic Early Diagnoses
Optics
Sample
Detection
Processing
© Israel Flores 2016
Photonic Early Diagnoses
Combine different wavelength lasers and shooting them at the sample
The brains of the operation, interpret detection results from the process
Detecting changes in wavelength
Move sample through disc and position across laser beam
Optics
Sample
Detection
Processing
© Israel Flores 2016
Photonic Early Diagnoses Enclosure
Sample
Detection
Processing
Optics
© Israel Flores 2016
Sample
© Israel Flores 2016
Processing
© Israel Flores 2016
Photonic Early Diagnoses Enclosure
Optics
Sample
Detection
Processing
© Israel Flores 2016
Prototype I (2015) Prototype II (2016)
© Israel Flores 2016
System Integration
Israel Flores
© Israel Flores 2016
Up to Speed
© Israel Flores 2016
Progress Report• New Library
• Camera Interface• Successful Data
Frame Exchange
Team Friendly Software for rapid test script development
© Israel Flores 2016
Outlook
© Israel Flores 2016
Budget
© Israel Flores 2016
Gantt Chart Spring 2016
Enclosure System
Biodetection Fabrication technique
Microcomputer Stand alone system
Optics detection
Microfluidics Stage 1
Enclosure System Final Design
Biodetection
Microcomputer
Optics
Microfluidics
Final Device Complete5/19/1888 3/27/1924 2/3/1960 12/11/1995 10/18/2031
© Israel Flores 2016
…Thank You© Israel Flores 2016