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"Nina's Words-Indonesia" words of life and life-sciences
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TEKNOLOGI DNA REKOMBINAN (KLONING)Posted on !"#!"$!! % & 'o((ents
Tekno)ogi *+ reko(binan, ata- )ebih dikena) dengan isti)ah .k)oning
*+/0 K)oning (er-pakan (etode yang dig-nakan -nt-k (e(b-at rep)ika
genetik dari sat- seg(en *+, se) ata- organis( secara kese)-r-han0
*engan de(ikian, k)oning *+ dig-nakan -nt-k (enghasi)kan kopi gen
ata- seg(en *+0 K)oning perta(a ka)i di)ak-kan ada)ah do(ba *o))y di
Skot)andia pada tah-n !112 yang (enghasi)kan do(ba yang identik
dengan ind-knya!0
K)oning do(ba *o))y di)ak-kan dengan somatic nuclear cell
transfer 3S+'T40 Se) so(atic do(ba 5antan dia(bi) inti se)nya ke(-dian
di(as-kkan da)a( se) te)-r do(ba betina yang s-dah dihi)angkan inti
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se)nya0 6enerasi perta(a (enghasi)kan do(ba berkepa)a hita( yang
(irip dengan ind-k betina sedangkan generasi ked-a (enghasi)kan
do(ba p-tih (irip dengan ind-k 5antan!0
*a)a( d-nia 7ar(asi, *+ reko(binan dig-nakan -nt-k prod-ksi protein7ar(asetik seperti ins-)in, hor(on pert-(b-han, inter7eron, 89g)ob-)in
dan (asih banyak )agi dapat di)ihat pada tabe) I diba:ah ini0 *a)a(
bidang )ain, tekno)ogi *+ reko(binan 5-ga dig-nakan -nt-k
(enghasi)kan he:an dan tana(an transgenic0 ;e:an transgenik
diharapkan dapat (e(p-nyai si7at9si7at yang diinginkan seperti tahan
terhadap penyakit sedangkan tana(an yang tahan terhadap penyakit
ata- (engand-ng vita(in"protein tertent-0 Tana(an transgenic perta(a
ka)i dipasarkan tah-n !11
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dengan en=i( retriksi endon-k)ease ke(-dian digab-ngkan ke(ba)i
dengan en=i( )igase0 *+ reko(binan ke(-dian di(as-kkan da)a( se)
bakteri s-paya dapat (enghasi)kan protein target yang diinginkan0
Tahap9tahap pe(b-atan *+ reko(binan>
a0 Pe(i)ihan vektor
b0 Pe(otongan *+ target dan vektor
c0 Penyisipan *+ target pada vektor
d0 Trans7or(asi
e0 Se)eksi hasi) trans7or(asi#
Re7erensi>
!0 +ES'O0 $nd ed0 ;-(an ')oning> Ethica) Iss-e0 $ an Introd-ction0 Aanchester, !11!
bo-t these adsRATE THIS:
! ote
This entry :as posted in CIDE S'IE+'E0 Book(ark the per(a)ink0
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6 RESPONSES TO “TENOLO#I !NA REOMBINAN $LONIN#%”
!0 Aree | 01/31/2011 at 3:31 PM | Reply
yg sya pernah baca, do))y (e(p-nyai -(-r yg pendek dibandingkan dgn
do(ba nor(a)0 (engapa?
o &ara(onina | 02/01/2011 at 6:42 AM | Reply
*o(ba *o))y p-nya -(-r yang pendek karena inti se) yang diba:a
ada)ah inti se) so(atik ind-k 5antannya0
Aengapa bisa de(ikian? *i da)a( inti se) terdapat *+ yang bagian
-5-ngnya dit-t-p o)eh pasangan basa ber-)ang
3te)o(er40 Pen-t-p -5-ng ini se(akin t-a se), se(akin pendek dan
sete)ah (encapai pan5ang (ini(-( (aka se) tidak
dapat (e(be)ah ke(-dian (ati0 +ah, pan5ang te)o(er *o))y (engik-ti
te)o(er ind-k 5antannya sehingga -(-rnya pendek0
$0 ed)isa*ada | 02/25/2011 at 3:23 PM | Reply
Se(oga 7ar(asi S* bisa sesegera (-ngkin p-nya (ata k-)iah prktik-(
ttg ha) inibanyak ide$ kreati7 pastinya akan ber(-nc-)ankeep Fre
on
o &ara(onina | 03/11/2011 at 5:03 AM | Reply
a(ien$:e are going to do this
(a- 5d pe)opor st-dy c)-b9nya?
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#0 ed)isa*ada | 04/06/2011 at 3:18 AM | Reply
se(oga bisa ber5a)an dengan baik b- rencana prktk-( biotek nya
hah saya ingn 7ok-s di k-)iah pt sa(a nyari$ in7o ttg bbrp prshaan b-0
Se(oga ada adik$ ke)as yg terdorong dan terinspirasi -tk (e(b-atnya
b- di tengah$ ker5aan k-)iah yg padat0 hehehehe06B0
o &ara(onina | 04/08/2011 at 12:24 PM | Reply
Ok00ttp s(angatGG
5gn )-pa nyari per-sahaan H posisi di(ana ka(- bs berke(bang
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DNA Rekombinan
BAB I
PENDAHULUAN
A La!ar Be"akan#
Kema$uan %ibi%an# !ekno"o#i saa! ini su%a& berkemban# san#a! 'esa!, ban(ak'enemuan baru !en!an# bio"o#i mo"eku"ar, %ian!aran(a (ai!u a%an(a sis!em k"onin#
)is!em k"onin# i!u sen%iri meru'akan sua!u 'roses men#&asi"kan in%i*i%u+in%i*i%u %ari
$enis (an# sama i%en!ik seara #ene!ik Pa%a &e-an a!au !umbu&an !er!en!u
'en#k"onin#an !erben!uk seara a"ami (ai!u kebiasaan 'roses &e-an a!au !umbu&an
bere'ro%uksi aseksua" )e%an#kan %a"am bio!ekno"o#i, k"onin# meru$uk 'a%a berba#ai
Sign (e -p
https://wordpress.com/?ref=footer_bloghttps://wordpress.com/themes/coraline/http://void%280%29/https://wordpress.com/?ref=lofhttps://wordpress.com/?ref=footer_bloghttps://wordpress.com/themes/coraline/http://void%280%29/https://wordpress.com/?ref=lof
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usa&a (an# %i"akukan manusia un!uk men#&asi"kan sa"inan berkas DNA a!au #en, se" a!au
or#anisme
)eirin# berkemban#n(a i"mu 'en#e!a&uan !ersebu!, munu""a& i"mu (an#
mem'e"a$ari men#enai 'emben!ukan kombinasi ma!eri #ene!ik (an# baru %en#an ara'en(isi'an mo"eku" DNA ke %a"am sua!u *ek!or se&in##a memun#kinkann(a un!uk
!erin!e#rasi %an men#a"ami 'erban(akan %a"am sua!u se" or#anisme "ain (an# ber'eran
seba#ai se" inan# (an# %ikena" seba#ai !ekno"o#i DNA rekombinan a!au biasa %isebu!
reka(asa #ene!ika .ekno"o#i ini memun#kinkann(a %i'ero"e&n(a sua!u 'ro%uk %en#an
si/a! !er!en!u %a"am -ak!u "ebi& e'a! %an $um"a& "ebi& besar %ari'a%a 'ro%uksi
seara kon*ensiona" ser!a mema%ukan si/a! %ari %ua $enis or#anisme (an# berbe%a
or#anisme !rans#enik %an "ain+"ain
Un!uk "ebi& $e"as men#enai DNA rekombinan, akan %i ba&as 'a%a 'emba&asanmaka"a& ini
B Rumusan asa"a&
Ber%asarkan "a!ar be"akan# %i a!as, maka %a'a! %irumuskan 'ermasa"a&an seba#ai
beriku! 3
1 A'aka& (an# %imaksu% DNA rekombinan
2 Ba#aimanaka& !eknik5!a&a'an !ekno"o#i DNA rekombinan
6 A'aka& man/aa! %ari !ekno"o#i DNA rekombinan
.u$uan
.u$uan %ari 'enu"isan maka"a& ini a%a"a& seba#ai beriku! 3
1 Un!uk men#e!a&ui 'en#er!ian %ari DNA rekombinan
2 Un!uk men#e!a&ui !eknik (an# %i#unakan %a"am !ekno"o#i DNA rekombinan
6 Un!uk men#e!a&ui man/aa! %ari !ekno"o#i DNA rekombinan
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D an/aa!
an/aa! %ari 'enu"isan maka"a& ini a%a"a& seba#ai beriku! 3
1 Ba#i ma&asis-a %a'a! me"a!i& ke!eram'i"an %an kemam'uan %a"am membua! kar(a !u"is
i"mia&
2 Ba#i 'embaa %a'a! menamba& sumber in/ormasi !en!an# !ekno"o#i DNA rekombinan
BAB II
PEBAHA)AN
A De/inisi DNA Rekombinan
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enuru! o&en %an Bo(er 18903 DNA rekombinan rDNA a%a"a& ben!uk DNA
bua!an (an# %ibua! %en#an men##abun#kan %ua a!au "ebi& sekuens (an# !i%ak akan
biasan(a !er$a%i bersama+sama Da"am &a" mo%i/ikasi #ene!ik, i!u a%a"a& %ii'!akan
me"a"ui 'en#ena"an DNA (an# re"e*an ke %a"am DNA or#anisme (an# a%a, se'er!i
'"asmi% bak!eri, un!uk ko%e un!uk a!au men#uba& si/a! berbe%a un!uk !u$uan !er!en!u,se'er!i resis!ensi an!ibio!ik Ne-s e%ia"Ne!
Rober! 2008 men#a!akan ba&-a DNA rekombinan a%a"a& DNA (an# men#a"ami
'eruba&an karena 'en(isi'an sua!u sekuens %euksinuk"eo!i%a (an# sebe"umn(a !i%ak
!er%a'a! %a"am mo"eku" DNA (an# su%a& a%a %en#an ara en:ima!ik a!au kimia-i
Reka(asa #ene!ika a%a"a& seran#kaian !eknik un!uk memo%i/ikasi %an merekomen%asi
#en %ari berba#ai or#anisme (an# berbe%a (an# $u#a %isebu! !ekno"o#i DNA
rekombinan
.ekno"o#i (an# %ikena" seba#ai !ekno"o#i DNA rekombinan, a!au %en#an is!i"a&
(an# "ebi& 'o'u"er reka(asa #ene!ika, ini me"iba!kan u'a(a 'erban(akan #en !er!en!u %i
%a"am sua!u se" (an# bukan se" a"amin(a se&in##a serin# 'u"a %ika!akan seba#ai k"onin#
#en Ban(ak %e/inisi !e"a& %iberikan un!uk men%eskri'sikan 'en#er!ian !ekno"o#i DNA
rekombinan )a"a& sa!u %i an!aran(a, (an# mun#kin 'a"in# re'resen!a!i/, men(ebu!kan
ba&-a !ekno"o#i DNA rekombinan a%a"a& 'emben!ukan kombinasi ma!eri #ene!ik (an#
baru %en#an ara 'en(isi'an mo"eku" DNA ke %a"am sua!u *ek!or se&in##a
memun#kinkann(a un!uk !erin!e#rasi %an men#a"ami 'erban(akan %i %a"am sua!u se"
or#anisme "ain (an# ber'eran seba#ai se" inan# Reka(asa #ene!ika %a'a! memberikan
&asi" (an# san#a! men#un!un#kan )eba#ai on!o& 'asien (an# men%eri!a %iabe!es !i%ak
mam'u memben!uk &ormon insu"in un!uk men#a!ur ka%ar #u"a %a"am %ara& ;"e& karena
i!u, 'asien membu!u&kan sun!ikan insu"in seba#ai !amba&an Den#an !eknik reka(asa
#ene!ika, 'ara 'ene"i!i ber&asi" memaksa mikroor#anisme bak!eri un!uk memben!uk
insu"in (an# miri' %en#an insu"in manusia sur(o, 2001
B .ekno"o#i DNA Rekombinan
.ekno"o#i DNA rekombinan meru'akan !eknik ke$uru!eraan #ene!ik (an#
me"iba!kan 'en##unaan DNA rekombinan + (ai!u DNA (an# men##abun#kan mak"uma!#ene!ik %ua s'eies or#anisme (an# ber"ainan
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1 ani'u"asi DNA in *i!ro "uar se" or#anisma
2 =abun#an, a!au rekombinasi DNA sesua!u or#anisma %en#an DNA bak!eria %a"am
'"asmi% a!au bak!erio/ak
6 Pen#k"onan, a!au !eknik un!uk mere'"ikasi 'ro#eni (an# memba-a DNA rekombinan
Proses+'roses %ia!as 'er!ama ka"i %i"akukan o"e& Pau" Ber# %an AD Kaiser 'a%a
!a&un 1872 ereka ber$a(a memasukkan DNA 'rokario! ke%a"am bak!eria, kemu%ian
o"e& )N o&en %an Herber! Bo(er (an# ber$a(a men##abun#kan DNA or#anisme
eukario! bersama '"asmi% bak!eria
Kom'onen (an# %i#unakan %a"am !eknik DNA rekombinan %ian!aran(a en:im
res!riksi un!uk memo!on# DNA, en:im "i#ase un!uk men(ambun# DNA %an *ek!or un!uk
men(ambun# %an men#k"onkan #en %i %a"am se" &i%u', !rans'oson seba#ai a"a! un!uk
me"akukan mu!a#enesis %an un!uk men(isi'kan 'enan%a, 'us!aka #enom un!uk
men(im'an #en a!au /ra#men DNA (an# !e"a& %ik"onkan, en:im !ranskri'si ba"ik un!uk
membua! DNA ber%asarkan RNA, 'e"aak DNA a!au RNA un!uk men%e!eksi #en a!au
/ra#men DNA (an# %iin#inkan a!au un!uk men%e!eksi k"on (an# benar >ek!or (an#
serin# %i#unakan %ian!arn(a '"asmi%, kosmi% %an bak!erio/a# En:im res!riksi,
%i#unakan un!uk memo!on# DNA En:im res!riksi men#ena" %an memo!on# DNA 'a%a
sekuens s'esi/ik (an# 'an$an#n(a em'a! sam'ai enam 'asan# basa En:im !ersebu!
%ikena" %en#an nama en:im en%onuk"ease res!riksi
A%a %ua ba#ian !er'en!in# (an# se"a"u %i#unakan %a"am reka(asa #ene!ika (ai!useba#ai beriku! 3
1 En:im se"u"er5e""u"ar En:(mes
En:im (an# %i'akai %a"am memani'u"asi DNA %ian!aran(a a%a"a& 3
a en:im En%onuk"ease, (ai!u en:im (an# men#ena"i ba!as+ba!as sekuen nuk"eo!i%a
s'esi/ik %an ber/un#si %a"am 'roses res!ri!ion a!au 'emo!on#an ba&an+ba&an #ene!ik
Pen##unaan en:im ini (an# 'a"in# umum an!ara "ain 'a%a sekuen 'a"in%romik En:im ini
%iben!uk %ari bak!eri (an# %ibua! se%emikian ru'a se&in##a %a'a! mena&an 'en(usu'anDNA, se'er!i #enom ba!erio'&a#e
b DNA 'o"imerisasi, (ai!u en:im (an# biasa %i'akai un!uk men#+o'( DNA En:im ini
men#sin!esis DNA %ari se" in%ukn(a %an memben!uk DNA (an# sama 'ersis ke se" in%uk
barun(a En:im ini $u#a bisa %i%a'a!kan %ari berba#ai $enis or#anisme, (an# !i%ak
men#&erankan, karena semua or#anisme 'as!i &arus men#+o'( DNA mereka
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RNA 'o"imerisasi (ai!u en:im (an# ber/un#si un!uk ?membaa sekuen DNA %an
men#sin!esis mo"eku" RNA kom'"emen!er )e'er!i &a"n(a DNA 'o"imerisasi, RNA
'o"imerisasi $u#a ban(ak %i!emukan %i ban(ak or#anisme karena semua or#anisme &arus
?merekam? #en mereka
% DNA "i#ase meru'akan sua!u en:im (an# ber/un#si un!uk men(ambun#kan sua!u ba&an
#ene!ik %en#an ba&an #ene!ik (an# "ain on!o&n(a sa$a, en:im DNA "i#ase ini %a'a!
ber#abun# %en#an DNA a!au RNA %an memben!uk ika!an '&os'&o%ies!er baru an!ara
DNA a!au RNA (an# sa!u %en#an "ainn(a
e Re*erse !ransri'!ases a%a"a& en:im (an# ber/un#si memben!uk b"ue+'rin! %ari
mo"eku" RNA memben!uk DNA DNA kom'"emen!er En:im ini %ibua! %ari *irus RNA
(an# men#uba& #enom RNA mereka men$a%i DNA ke!ika mereka men#in/eksi inan#n(a
En:im ini biasa %i'akai ke!ika ber!emu %en#an #en eukario!ik (an# biasan(a !er'isa&+
'isa& men$a%i 'o!on#an kei" %an %i'isa&kan o"e& in!rons %a"am kromosom
2 >ek!or na!ura"5 Na!ura" >e!ors
)eba#ai sa"a& sa!u ara un!uk memani'u"asi DNA %i "uar se", 'ara i"mu-an %a"am
bio!ekno"o#i &arus bisa membua! sua!u !em'a! (an# kea%aann(a s!abi" %an ook
%en#an !em'a! DNA (an# %imani'u"asi )eka"i "a#i, a"am !e"a& memberikan so"usi %ari
masa"a& ini >ek!or %isini bisa %iar!ikan seba#ai a"a! (an# memba-a DNA ke %a"am se"
in%uk barun(a
A#ar sua!u me!o%e %a"am reka(asa #ene!ika %ian##a' ber&asi", %i %a"am *ek!or, DNA&asi" rekombinan se&arusn(a benar+benar &an(a %iba-a se!e"a& sebe"umn(a DNA
rekombinan %i#abun#kan %en#an DNA *ek!or me"a"ui en:im "i#ase Namun %i %a"am
*ek!or, DNA rekombinan !i%ak !ermu!asi "a#i memben!uk DNA %en#an si/a! baru
on!o& %ari *ek!or na!ura" %ari a"am a%a"a& '"asmi% %an *irus a!au
ba!erio'&a#e @i!ar!o, 200
A%a'un !eknik 'embua!an DNA rekombinan a%a"a& seba#ai beriku!3
1 .eknik men#iso"asi DNA
2 .eknik memo!on# DNA %en#an men##unakan en:im re!riksi en%onuk"ease
6 .eknik men##abun#5 men(ambun# DNA %en#an men##unakan en:im "i#ase
4 .eknik memasukkan DNA ke%a"am se" &i%u' *ek!or
>ek!or berkemban# %en#an se"eksi DNA (an# %ireka(asa
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=ambar 13 mekanisme DNA Rekombinan
1 Iso"asi DNA (an# %ia-a"i %en#an me"akukan 'erusakan ser!a 'en#&i"an#an %in%in# se"Da"am 'roses ini %a'a! %i"akukan seara mekanis a!au'un %en#an ara en:ima!is)e!e"a& 'erusakan se" !e"a& %i"akukan, "an#ka& se"an$u!n(a a%a"a& 'e"isisan se" &a" ini
%a'a! %i"akukan %en#an men##unakan bu//er nonosmo!ik, ser!a %e!er$en (an# kua!se'er!i !ri!on +100 a!au %en#an so%ium %o%esi" su"/a! )D) Remukan se" (an#%iakiba!kan o"e& "isisn(a se" %ibuan# %en#an me"akukan sen!ri/u#asi se&in##a bias%ibe%akan an!ara ba#ian (an# rusak ser!a or#ane" !ar#e! (an# 'a%a ak&irn(a%i%a'a!"kan DNA (an# nan!in(a %i"akukan 'emurnian %en#an 'enamba&an amoniumase!a! %an a"o&o"
2 Pemo!on#an mo"eku" DNA, baik #enomik mau'un '"asmi% %i"akukan %en#an en:imres!rik
si A%a
%ua maam en:im res!riksi (aieu en:im res!riksi !i'e I %anen:im res!riksi !i'e II En:im res!riksi !i'e II mem'un(ai si/a!+si/a! umum(an# 'en!in# seba#ai beriku!3
a men#ena"i uru!an !er!en!u se'an$an# em'a! &in##a !u$u& 'asan# basa%i %a"am mo"
eku" DNA
b memo!on# ke%ua un!ai mo"eku" DNA %i !em'a! !er!en!u 'a%a a!au %i %eka!!em'a!
'en#ena"ann(a
men#&asi"kan /ra#men+/ra#men DNA %en#an berba#ai ukuran %an uru!anbasa
Ber%asarkan !em'a! &asi" 'emo!on#an, /ra#men+/ra#men DNAmemi"iki %ua maamu$un# (ai!u3
U$un# "en#ke! s!i,k( en% a!au u$un# ko&esi/, !er$a%i $ika !em'a!'emo!on#an
'a%a ke%ua un!ai DNA !er'isa& se$au& bebera'a 'asan# basa,se&in##a men#&asi"kan
/ra#men+/ra#men %en#an u$un# ? (an# runin#karena masin#+masin#
un!ai !un##a"n(a men$a%i !i%ak sama
'an$an# Dua/ra#men DNA %en#an u$un# (an# runin# akan mu%a& %isambun#kansa!u
sama "ain
U$un# !um'u" b"un! en% , !er$a%i $ika !em'a! 'emo!on#an DNA 'a%a'osisi (an# sama
Ke%ua /ra#men &asi" 'emo!on#ann(a akan mem'un(ai u$un# ? (an# !um'u" karena
masin#+masin# un!ai !un##a"n(a sama'an$an#n(a
Pen(a!uan %ua /ra#men DNA u$un# !um'u" su"i! %i"akukanse&in##a memer"ukan
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'er"akuan !amba&an, misa"n(a 'emberian mo"eku""inker, mo"eku" a%a'!or, a!au 'enamba&
an en:im %eoksinuk"eo!i%i"!rans/erase
6 .a&a' se"an$u!n(a a%a"a& 'en(ambun#an mo"eku" DNA
A%a !i#a ara (an#%a'a! %i#unakan un!uk me"i#asi /ra#men+/ra#men DNA seara in*i!ro Per!ama,"i#asi men##unakan
en:im DNA "i#ase %ari bak!eri Ke%ua, "i#asi men##unakanDNA "i#ase %ari se"+se" E
,o"i (an# !e"a& %iin/eksi %en#an bak!erio/a# . 4 a!au"a:im
%isebu! seba#ai en:im . 4 "i#ase Ke!i#a (ai!u 'emberian en:im%eoksinuk"eo!i%i" !rans/e
rase un!uk men(in!esis un!ai !un##a" &omo'o"imerik 6?
)u&u o'!imum ba#i ak!i*i!as DNA "i#ase sebenarn(a
67C Akan !e!a'i, 'a%asu&u ini ika!an
&i%ro#en (an# seara a"ami !erben!uk %i an!ara u$un#+u$un#"en#ke!
akan men$a%i !i%ak s!abi" %an
kerusakan akiba! 'anas akan !er$a%i 'a%a!em'a! ika!an !ersebu! ;"e& karena i!u, "i#a
si biasan(a %i"akukan 'a%a su&uan!ara 4 %an 1C %en#an -ak!u inkubasi
reaksi (an# %i'er'an$an# serin# ka"i&in##a sema"am
4 )e!e"a& !a&a' 'en(ambun#an mo"eku"
DNA, %i"akukan ana"isis !er&a%a' &asi"'emo!on#an DNA #enomik %an DNA *ek!or ser!a
ana"isis &asi" "i#asi mo"eku"+mo"eku" DNA !ersebu! men##unakan !eknik e"ek!ro/oresis
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(an#memba-a DNA rekombinan masi& &arus %i"akukan se"eksi
un!uk men%a'a!kanse" (an# DNA rekombinann(a memba-a /ra#men sisi'an a!au #en
(an#
%iin#inkan Pa%a %asarn(a a%a !i#a kemun#kinan (an# %a'a! !er$a%i se!e"a&!rans/ormasi
%i"akukan, (ai!u3
1 )e" inan# !i%ak %imasuki DNA a'a 'un a!au berar!i !rans/ormasi #a#a"
2 )e" inan# %imasuki *ek!or re"i#asi a!au berar!i "i#asi #a#a", %an
6 )e" inan# %imasuki *ek!or rekombinan %en#an5!an'a /ra#men sisi'an a!au#en
(an# %iin#inkan
)e"eksi se" rekombinan (an# memba-a /ra#men (an# %iin#inkan %i"akukan %en#an
menari/ra#men !ersebu! men##unakan /ra#men 'e"aak 'robe,(an# 'embua!ann(a %i"akukan
seara in
*i!ro men##unakan !eknik reaksi 'o"imerisasi beran!ai a!au 'o"(merase &ain rea!
ion PR
Pe"aakan/ra#men (an# %iin#inkan an!ara "ain %a'a! %i"akukan me"a"ui ara (an#%ina
makan &ibri%isasi ko"oni .$a&$o"eksono, 2008
.rans/er DNA a!au 'er'in%a&an DNA ke %a"am bak!eri %a'a! me"a"ui !i#a ara
(ai!u3
1 .rans/ormasi 3 !rans/er DNA (an# umumn(a berasa" %ari sa!u se" bak!eri ke %a"am se"
(an# berbe%a Prosesn(a a%a"a& ke!ika sebua& se" bak!eri 'ea& a!au "isis maka DNA
sirku"ar akan !er"e'as ke "in#kun#an E/isiensi !rans/ormasi ber#an!un# 'a%a
kom'e!ensi se"
2 Kon$u#asi 3 meru'akan 'emin%a&an ma!eri #ene!ik beru'a '"asmi% seara "an#sun#
me"a"ui kon!ak se" %en#an memben!uk s!ruk!ur se'er!i $emba!an %ian!ara %ua se"
bak!eri (an# ber%eka!an Umumn(a !er$a%i 'a%a bak!eri #ram ne#a!i/
6 .rans%uksi 3 !rans/er ma!eri #ene!ik %ari sa!u bak!eri ke bak!eri "ainn(a %en#an
men##unakan *irus bak!eri seba#ai *ek!or .rans/er ini men##unakan 'rinsi' %asar %ari
#a"ur %onor (an# men(e%iakan DNA ba#i #a"ur resi'ien Perbe%aan u!aman(a %en#an
!rans/er DNA "ainn(a a%a"a& DNA %i!rans/er me"a"ui 'eran!araan bak!erio/a#
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an/aa! .ekno"o#i DNA Rekombinan
Berba#ai 'ene"i!ian !en!an# DNA rekombinan ban(ak #unakan %an %iman/aa!kan
%a"am berba#ai bi%an#, %ian!aran(a 3
1 Bi%an# in%us!ri
Pene"i!ian reka(asa #ene!ika %i bi%an# in%us!r( se%an# menin#ka! %en#an e'a!
Berba#ai usa&a (an# !e"a& #ia! %i"akukan misa"n(a 3
1 eni'!akan bak!eri (an# %a'a! me"aru!kan "o#am+"o#am "an#sun# %ari %a"am bumi
2 eni'!akan bak!eri (an# %a'a! men#&asi"kan ba&an kimia
eni'a!kan bak!eri (an# %a'a! men#&asi"kan ba&an men!a& kimia se'er!i e!i"en
(an# %i'er"ukan un!uk 'embua!an '"as!i
2 Bi%an# Per!anian
Bebera'a man/aa! reka(asa #ene!ika %a"am bi%an# 'er!anian %ian!aran(a a%a"a&3
1 en##an!i 'emakaian 'u'uk ni!ro#en (an# ban(ak %i'er#unakan !a'i ma&a" &ar#an(a,
o"e& /iksasi ni!ro#en seara a"amia& Bak!eri !ana& R&i:obium s' %a'a! men#a%akan
in/eksi ke %a"am akar %ari !anaman /ami"( Le#uminosae In/eksi ini men#&asi"kan bin!i"akar %an bak!eri (an# !er%a'a! %i %a"amn(a %a'a! men#ika! :a! "emas bebas %ari u%ara
un!uk %i uba&n(a men$a%i ni!ro#en (an# %a'a! %iambi" %an %i#unakan o"e& !anaman
!ersebu!
2 .eknik reka(asa #ene!ika men#usa&akan !anaman+!anaman !eru!ama (an# mem'un(ai
ar!i ekonomi (an# !i%ak be#i!u 'eka& !er&a%a' 'en(aki! (an# %isebabkan o"e& bak!eri,
$amur %an ain#
6 en#usa&akan !anaman+!anaman (an# mam'u men#&asi"kan 'e'!isi%a sen%iri
6 Bi%an# Pe!ernakan
1 .e"a& %i'ero"e& *aksin+*aksin un!uk me"a-an 'en(aki! menre! #anas (an# %a'a!
mema!ikan anak+anak babi
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2 )u%a& %i'asarkan *aksin (an# e/ek!i/ !er&a%a' 'en(aki! kuku %an mu"u!, (ai!u
'en(aki! #anas %an san#a! menu"ar 'a%a sa'i, %omba, kambin#, rusa %an babi
4 Bi%an# Ke%ok!eran
=en+#en ba#i bebera'a 'ro!ein (an# %ibu!u&kan %a"am bi%an# ke%ok!eran (an#%ibu!u&kan %a"am bi%an# ke%ok!eran (ai!u 'embua!an insu"in manusia o"e& bak!eri
Es&re&ia o"i un!uk 'en#oba!an 'en(aki! %iabe!es @iki'e%iaor#
BAB III
PENU.UP
A Kesim'u"an
Ber%asarkan 'emba&asan 'a%a maka"a& ini maka %a'a! %i!arik bebera'a
kesim'u"an seba#ai beriku! 3
1 DNA rekombinan a%a"a& DNA (an# men#a"ami 'eruba&an karena 'en(isi'an sua!u
sekuens %euksinuk"eo!i%a (an# sebe"umn(a !i%ak !er%a'a! %a"am mo"eku" DNA (an#
su%a& a%a %en#an ara en:ima!ik a!au kimia-i
2 Kom'onen (an# !er"iba! %a"am Dna rekombinasi (ai!u En:im res!riksi un!uk memo!on#
DNA, DNA 'o"(merase, en:im "i#ase, en:im DNA "i#ase, >ek!or un!uk men(ambun# %an
men#k"onkan #en %i %a"am se" &i%u', .rans'oson, Pus!aka #enom, En:im !ranskri'si
ba"ik, Pe"aak DNA a!au RNA
6 .a&a'an+!a&a'an !ekno"o#i DNA rekombinan a%a"a& iso"asi DNA kromosom (an# akan
%ik"on, 'emo!on#an mo"eku" DNA men$a%i se$um"a& /ra#men %en#an berba#ai ukuran,
iso"asi DNA *ek!or, 'en(isi'an /ra#men DNA ke %a"am *ek!or un!uk men#&asi"kan
mo"eku" DNA rekombinan, !rans/ormasi se" inan# men##unakan mo"eku" DNA
rekombinan, reiso"asi mo"eku" DNA rekombinan %ari se" inan#, %an ana"isis DNA
rekombinan
B )aran
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aka"a& ini masi& $au& %ari kesem'urnaann(a %an in/ormasin(a 'un !erba!as ;"e&
karena i!u, 'en(usun men(arankan a#ar 'embaa menari "i!era!ure "ain (an# memba&as
men#enai $u%u" %ari maka"a& ini
DAF.AR PU).AKA
Ans&ori DNA Rekombinan on"ine .erse%ia &!!'355ans&oriomu*om5
DNARekombinan&!m" .an##a" 1 No*ember 2012
I"&am 2008 DNA rekombinan on"ine .erse%ia &!!'355i"&am+
#an!:b"o#s'o!om520085065%na+rekombinan&!m" .an##a" 1 No*ember 2012
Ne-s e%ia", 2012 DNA Rekombinan .erse%ia &!!'355---ne-s+
me%ia"ne!5&ea"!&5Reombinan!+DNA+@&a!+is+Reombinan!+DNA+29In%onesian
28as'G .an##a" 1 No*ember 2012
.$a&$o"eksono, Aris, 2008 DNA Rekombinan ;n"ine .erse%ia&!!'355-ebi'bai%5+
!'b5/i"es5ma!eri5#ene!ika5%na5rekombinan&!m
http://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://anshori.comuv.com/%20DNA_Rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://ilham-gantz.blogspot.com/2009/03/dna-rekombinan.htmlhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspxhttp://www.news-medical.net/health/Recombinant-DNA-What-is-Recombinant-DNA-(Indonesian).aspx
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Ri/a?I, u&aimin P&De%) 2010 =ene!ika Rekombinasi %an Po'u"asi =a"aG( )iene a"an#
)a!riani 2011 DNA rekombinan on"ine
.erse%ia &!!'355sa!riani08n#eb"o#b"o#s'o!om520115125%na+rekombinan&!m" .an##
a" 1 No*ember 2012
@iki'e%ia 2011 Re,ombinan! DNA ;n"ine
.erse%ia&!!'355en-iki'e%iaor#5-iki5Reombinan!DNA .an##a" 1 No*ember 2012
@iki'e%ia 2011 .ekno"o#i DNA rekombinan ;n"ine
.erse%ia&!!'355ms-iki'e%iaor#5-iki5.ekno"o#iDNArekombinan .an##a" 1
No*ember 2012
@i!ar!o, AB, 200 Ins'ira!or Kema$uan I'!ek Pusa! Pene"i!ian Bio!ekno"o#i LIPI ;n"ine
.erse%ia &!!'355---beri!ai'!ekom5:beri!a+beri!ai'!ek+200.an##a" 1 No*ember
2012
Di'oskan o"e& Pu$o @inar!o %i 16
Kirimkan Ini "e-a! Emai" B"o#.&is Berba#i ke .-i!!erBerba#i ke FaebookBa#ikan ke
Pin!eres!
http://satriani09ngeblog/http://en.wikipedia.org/wiki/Recombinant_DNAhttp://ms.wikipedia.org/wiki/Teknologi_DNA_rekombinanhttp://www.beritaiptek.com/zberita-beritaiptek-2005https://plus.google.com/115748711375140916374http://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=emailhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=emailhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=bloghttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=bloghttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=twitterhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=facebookhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinteresthttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinteresthttp://satriani09ngeblog/http://en.wikipedia.org/wiki/Recombinant_DNAhttp://ms.wikipedia.org/wiki/Teknologi_DNA_rekombinanhttp://www.beritaiptek.com/zberita-beritaiptek-2005https://plus.google.com/115748711375140916374http://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=emailhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=bloghttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=twitterhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=facebookhttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinteresthttps://www.blogger.com/share-post.g?blogID=2956093438734974330&postID=6708615787062431531&target=pinterest
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.i%ak a%a komen!ar3
Poskan Komen!ar
Pos!in# Lebi& BaruBeran%aLan##anan3 Poskan Komen!ar A!om
Arsip Blog
2014 2! are! 1
Februari 1
DNA
Rekombinan
Mengenai Saya
Pujo Winarto
Li&a! 'ro/i" "en#ka'ku
http://catatannkuliahbiologi.blogspot.co.id/2014/03/bunga-buah-dan-biji.htmlhttp://catatannkuliahbiologi.blogspot.co.id/http://catatannkuliahbiologi.blogspot.com/feeds/6708615787062431531/comments/defaulthttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/search?updated-min=2014-01-01T00:00:00-08:00&updated-max=2015-01-01T00:00:00-08:00&max-results=2http://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_03_01_archive.htmlhttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_02_01_archive.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014_02_01_archive.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://plus.google.com/115748711375140916374https://plus.google.com/115748711375140916374http://catatannkuliahbiologi.blogspot.co.id/2014/03/bunga-buah-dan-biji.htmlhttp://catatannkuliahbiologi.blogspot.co.id/http://catatannkuliahbiologi.blogspot.com/feeds/6708615787062431531/comments/defaulthttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/search?updated-min=2014-01-01T00:00:00-08:00&updated-max=2015-01-01T00:00:00-08:00&max-results=2http://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_03_01_archive.htmlhttp://void%280%29/http://catatannkuliahbiologi.blogspot.co.id/2014_02_01_archive.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttp://catatannkuliahbiologi.blogspot.co.id/2014/02/dna-rekombinan.htmlhttps://plus.google.com/115748711375140916374https://plus.google.com/115748711375140916374
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