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Biology of ethylene production & action in fruits

What is ethylene?

C2H4 Very simple molecule A gas An important chemical feedstock A natural plant hormone

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Where does ethylene come from?

Smoke Vehicle exhausts Ripening rooms Ripening fruits

Ethylene - an important factor

Useful: Accelerates ripening Causes abscission

A problem: Accelerates ripening Accelerates senescence Causes abscission

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Uses of ethylene in horticulture

Induction of flowering Bulbs, Pineapple & other Bromeliads

Harvest aid Walnuts, Sour cherries

Induction of ripening or coloring Bananas, Citrus

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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History of ethylene biology

Prehistoric Fruit ripening, smoky

rooms, ripening fruit Amos, 1000 B.C.

Scarification of figs -wound ethylene

Neljubow, 1907 Ethylene gas - plant

growth regulator Cousins, 1913

Ethylene causes ripening

Gane, 1932 Produced by ripening

fruits

Goeschl and Pratt, 1960 Role in plant growth and

development Plant hormone

Veen, 1978 Silver thiosulfate

Yang, 1979 Ethylene biosynthesis

pathway Bleeker, 1988

Etr-1 Sisler and Blankenship, 1996

1-MCP

Ethylene responses

Reduction in growth (seedlings) Loss of leaves and flowers (plants) Leaf yellowing or death (plants) Epinasty (leaves) Senescence (flowers) Ripening (fruits) Abscission (fruits, leaves, branches) Dehiscence (seeds)

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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EthyleneEthylene

AirAir

Neljubow, a graduate student in Russia, was the first to show that ethylene caused these strange effects on etiolated pea seedlings

Seedling growth

Carnation model system

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Characteristics of ethylene responses

Threshold concentration (0.1 ppm) Plateau concentration (10 ppm) Associated respiration rise Temperature optimum (15 - 25 C) CO2 (>1%) inhibits

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Abscission of snapdragon flowers in response to ethylene shows a typical threshold and plateau

response

The ethylene response cascade

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Respiration - important physiological indicator

Climacteric fruits Respiration and

ethylene production rises during ripening

Ripening induced by ethylene

Bananas, apples, avocados, tomatoes, pears, many tropicals

Non-climacteric fruits

Respiration falls steadily throughout development

Ethylene not involved in ripening

Citrus, grapes, olives, cherries, many berries 0

20

40

60

80

100

120

140

1 4 7 10 13 16 19 22 25 28

Time (days)

Res

pir

atio

n (

mg

CO

2/kg

/hr)

Strawberry

TomatoTomato

Strawberry

Respiration and ethylene production rise during fruit ripening

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Ethylene is a ripening ‘trigger’in climacteric fruits Once ripening is initiated,

climacteric fruits produce ethylene Ripening is then self-controlled

The Yang Cycle

MET

SAM synthase

SAMACC

S-methylthioadenosine

MACC

malonyl ACC

ACC synthase

Ethylene

S-methyl thioribose

-

N-malonyltransferase

ACC oxidase

AVG/AOA

Low O2

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Tools for working with ethylene: Measurement

Expensive, but routine Bioassay - cheap, difficult Kitagawa tubes - $2 / measurement Proprietary analysers - $500 -

$10,000 Gas chromatograph - $10,000 -

$30,000 Photo-acoustic detector - $50,000

OvenDetector

Electrometer

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Sensorsense Photoacoustic Ethylene Detector

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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A nanotechnology ethylene detector

Based on the ethylene binding site Carbon nanotubes ‘doped’ with

copper When ethylene binds, the electrical

properties of the nanotubes change

Tools for working with ethylene: Application

Ripening fruits Ethylene gas Gas, closed space Acetylene, CO Ethephon

Liquid, spray, drench

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Unsprayed Gravenstein Sprayed

hephon-treated

Control

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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2CaO + 2C = Ca2C + CO2 CaC2 + H2O = C2H2 (+ C2H4) + CaO

Molecular manipulation of ripeningNH3

+

CH3-S-CH2-CH2-CH-COO-

Methionine

MET

NH3+

CH3-S-CH2-CH2-CH-COO-

Adn

+

SAM

S-adenosyl methionineACC

1-aminocyclopropane-1-carboxylic acid

H2C CH2

C-OOC NH3

+

EthyleneCH2=CH2

SAM synthase

ACC synthase

ACC oxidase

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Molecular manipulation of ripening

Anti-sense ACC synthase Anti-sense ACC oxidase Result - fruits that ripen very slowly,

require ethylene treatment to ripen Just like Never Ripe (NR), a tomato

mutant, used to develop long shelf-life tomatoes

Anti-sense ACC oxidase in melons

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Anti-sense technology will improve marketing of many fruits

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis

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Questions?

Reid, Michael "Biology of Ethylene Production and Action in Fruits" (c) 2015 Postharvest Technology Center, UC Davis