8/3/2019 Biology Lab (Sept 28)

http://slidepdf.com/reader/full/biology-lab-sept-28 1/3

Jordan Cameron

Scott Swartzentruber and Callum Walter

September 2

Indentifying Nutrients Investigation

Abstract: In this activity, we were to use laboratory tests to identify simple sugars(monosaccharides) starches, and lipids. We then used these tests to identify the

nutrients present in the unknown sample.

Introduction:

Purpose: The study was performed to try to figure out what the unknown solution was.

This is achieved by running a series of tests detecting which nutrients are present in the

sample.

Hypothesis: My prediction is that the unknown sample is a type of dairy product. Just

from observation, it was observed that the unknown sample shares many traits with

milk, like the density, the smell, and the colour. The unknown sample has a brownish

hue, so I am guessing that it is a type of nut milk alternative or something of the sort.

Materials:

  Goggles

  Thermometer

  Test-tube holder

  Detergent solution

  Test Tubes

  400-ml beaker

  Medicine droppers

  Ring clamp

  Test tube racks

  10-ml graduated cylinder

  Depression spot plate

  Utility stand

  Hot Plate

  Distilled water

  Rubber stoppers

Procedure: Refer to “Identifying Nutrients Investigation”, will be attached. 

8/3/2019 Biology Lab (Sept 28)

http://slidepdf.com/reader/full/biology-lab-sept-28 2/3

 

Observations:

Monosaccharide test

Chemical Reaction

5% glucose (dextrose) solution - turned orange, then brown- opaque

5% fructose solution - turned orange, then brown

- opaque

5% sucrose solution - turned lime green

- translucent

5% starch suspension - no change

Unknown solution - turned light brown/yellow

Starch Test

Chemical Reaction5% glucose (dextrose) solution - turned orange, then brown

- opaque

5% sucrose solution - turned orange, then brown

- opaque

5% starch suspension - turned lime green

- translucent

Unknown solution - no change

Water - turned light brown/yellow

Sudan IV Lipid TestChemical Reaction

Unknown solution - turned a bright, pale purple

- opaque

Milk - turned a bright, pale purple

- opaque

Water - turned burgundy

- translucent 

Vegetable oil - turned red

- translucent 

Translucence Lipid Test

After doing the test, only the vegetable oil made the paper appear to be translucent.

8/3/2019 Biology Lab (Sept 28)

http://slidepdf.com/reader/full/biology-lab-sept-28 3/3

 

Analysis:

-  Questions:

a)  The evidence that suggests not all sugars are monosaccharides is that during the

monosaccharide test, sucrose didn’t turn as dark (it turned a bright lime green),

meaning there is a lesser concentration of sugar in it.b)  It provides more evidence to support your results. Also, the Sudan IV lipid test

gives more information than the translucence lipid test.

g) With the added sugar cube into all the chemicals, the results would be very

different. It would make it look like they all had sugars in them, which isn’t true. 

h) I predict that the colour of the iodine would turn blue. I think this because the

reaction between the iodine and the starches in the paper would cause that

reaction.

 j) You would use Benedict’s Solution, because that is the solution used to test for

monosaccharides. You could also use Lugol’s solution, and check for starches in

the artificial sweeteners.

m) There are many careers you could use this knowledge in, for example, a

nutritionist, a doctor, or a biologist are all very good examples.