Biology: A Biology: A Molecular Molecular Approach, Approach, LaboratoryLaboratory

Lab 5

BusinessBusinessAttendanceHandouts

BusinessBusinessQuiz

BusinessBusinessHand back Lab Math Problems

BusinessBusinessTurn in Microscope Homework

BusinessBusinessMosquito Webquest

◦If not turned in at this point, is a zero

◦Will have them graded with feedback for you by next week’s lab

BusinessBusiness

Mosquito Self Serve Trapping

◦How to Operate the Mosquito Traps

BusinessBusiness

What’s Due Next Week

◦Quiz Micropipetting Solutions and Lab Math

MicropipettingMicropipetting

Let’s organize our lab groups

Get your lab notebookGet a lab coat

MicropipettingMicropipetting

Watch me demonstrate how to use a micropipette

MicropipettingMicropipetting

Pull out “Your Measuring Small Volumes and Mass” handout and Let’s Get Busy!

SolutionsSolutionsWhat is a solution?

◦Solute

◦Solvent

SolutionsSolutionsMaking a Solution Demonstration

SolutionsSolutionsWhy do we use solutions in a

biotech lab?

SolutionsSolutions

Pull out your “Solution Preparation and Dilutions” handout and Let’s Get Busy!

Micropipetting Activity•Measuring Small Volumes and Mass handout •Lab coat•Gloves•Lab notebook•Microcentrifuge (s) – Two if we have them•At each bench:

• dH2O in a 15 ml tube• 30 Eppendorf Tubes in a beaker• Micropipette, P-10 with tips• Micropipette, P-20 with tips• Micropipette, P-200 with tips• Micropipette, P-1000 with tips• 3 Tube racks• 3 Permanent markers• 3 black ink pens• 1weigh Balance• Beaker for used tips• Weigh papers

Solutions and Dilutions Activity•Copy of this PP•Solution Preparation and Dilutions handout•Two 500 ml beakers filled with 2/3 tap water•Food coloring with pipette•NaCl with spatula•2 glass stir bars