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Biochemistry Indicators
What is an indicator?
• Indicator- chemical that produces a characteristics color when a particular substance is present
• Standards- results that provide you with a positive test
Simple CarbohydrateBenedict’s Test
– Add a few drops of Benedict’s solution to what you are testing
– Put solution in boiling water for 2-3 minutes– Positive Result: Solution changes color- white, orange,
red
Complex Carbohydrate
Iodine Test– Add a few drops of iodine to the solution you are
testing– Positive Result: Solution turns black
Lipids
Transparency Test– Place a few drops of solution testing on a brown
paper bag– Positive Result: Transparent when held up to
light
Protein
Biuret Test– Add a few drops of biuret solution to what you are
testing– Positive Result: Solution turns from blue to violet