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Instructor: Gail Grabner ([email protected]) Topics: Protein purification, Dipeptide sequence analysis, Molecular cloning, ELISA, Lipid analysis. Student comments: “I took a lot out of this class; almost nothing was repetitive of anything I had learned in any other lab.” - PowerPoint PPT Presentation
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Biochemistry 455 Fall Biochemistry 455 Fall 20092009
Techniques in Biochemistry & Techniques in Biochemistry & BiotechnologyBiotechnology
Student comments:“I took a lot out of this class; almost nothing was repetitive of anything I had learned in any other lab.”“I feel that my lab skills improved after taking BIOC 455. I’ve gained more experience and learned a lot of techniques and assays.”“The techniques used here are genuinely applicable, particularly for students already doing research & considering graduate school.”“Things I learned in BIOC 455 will most definitely help me when I enter the workforce.”
Instructor: Gail Grabner ([email protected])Topics: Protein purification, Dipeptide sequence analysis, Molecular cloning, ELISA, Lipid analysis
Techniques in Biochemistry & Techniques in Biochemistry & BiotechnologyBiotechnology : BIOC 455
CREDITS: 4 hours
WHEN: M, W Lecture: 1:00-1:50; Lab: 2:00-4:50
WHERE: Lab in 218 Noyes Lab
INSTRUCTOR: Gail K. Grabner
CONTACT: [email protected]
BIOC 455: Course ContentBIOC 455: Course Content
Dipeptide analysis (2 lab periods) Determination of the amino acid sequence of a dipeptide
Photometry (1 lab period) Colorimetric quantitation of total protein concentration.
Study of the properties of β-galactosidase (4 lab periods) Kinetic assays of β-galactosidase
Study of carbohydrates (1 lab period) Enzymatic and chemical determination of carbohydrate unknowns
Purification of Glutamate Oxaloacetate Transaminase from pig heart muscle (5 lab periods) Three step purification of an active enzyme from a natural source.
BIOC 455: Course ContentBIOC 455: Course Content
Study of the interaction of RNA polymerase subunits. (1 lab period) Affinity pull-down of separately expressed RNA polymerase
subunits from Methanococcus jannaschii. Study of the properties of aspartate transcarbamylase (1
lab period) Kinetic assays of the allosteric enzyme ATCase
Use of the ELISA in immunology (3 lab periods) Determination of the titers of purified sera, unpurified sera, and
monoclonal ascites. Characterization of Erythrocyte Membranes (2 lab periods)
Extraction of protein and lipid fractions of pig red blood cells. In vitro transcription/translation (2 lab periods)
Coupled system to produce isotopically labeled protein product
BIOC 455: Student CommentsBIOC 455: Student Comments
BIOC 455: Student CommentsBIOC 455: Student Comments
BIOC 455: Student CommentsBIOC 455: Student Comments