Berry morphology and composition in irrigated and non-irrigated grapevine (Vitis vinifera L.)

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  • Journal of Plant Physiology 169 (2012) 1023 1031

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    Journal of Plant Physiology

    jou rn al h o mepage: www.elsev ie

    Berry morphology and composition in irrigated a(Vitis vinifera L.)

    Adriano Sofoa,, Vitale Nuzzoa, Giuseppe Tatarannia, Michele M

    a Dipartimento asilicab Dipartimento Potenc Dipartimento sano 4

    a r t i c l

    Article history:Received 7 November 2011Received in revised form 6 March 2012Accepted 7 March 2012

    Keywords:AglianicoAnthocyaninsFlavonolsIrrigationMetals

    The present study was carried out in a 5-year-old vineyard (Vitis vinifera L., cv. Aglianico) located in South-ern Italy. Half of the plants (IRR) were fully irrigated, whereas the other half were not irrigated (NIRR). Inboth of the treatments, plant water status, gas exchange, photosynthetic efciency and productive per-formance were determined. The arid conditions resulted in signicant decreases in stem water potentialin NIRR (minimum values of 1.34 and 1.52 MPa in IRR and NIRR, respectively). The values of yield perplant, cluster weight and total berry weight were signicantly higher in IRR. Grape berries were sepa-

    Introductio

    Among responsibleThey are loskins and l2007; Gam

    Abbreviatiospiration; ETo,photochemistrplants; PPFD, pleaf-to-air vapwater potentia

    Correspone dellAmbient85100 Potenza

    E-mail add

    0176-1617/$ http://dx.doi.orated into four weight classes, and morphometric and microscopic analyses were carried out to measureand calculate berry skin characteristics. Irrigation determined a marked shift toward heavier (+23% in theclass 1.25 g) and bigger (336.35 mm3 vs 299.15 mm3) berries, and induced signicant changes in othermorphometric berry parameters. No differences among berry weight classes and irrigation treatmentswere observed for berry skin thickness. In all of the berry weight classes, total anthocyanins extractedfrom berry skins were signicantly higher in NIRR than in IRR (12301.53 and 9585.52 mg kg1 fresh berryskin, respectively), and appeared to be positively related to berry weight, whereas total avonols werenot signicantly different between the two treatments. Qualitative changes in the levels of single antho-cyanin and avonol compounds were detected between IRR and NIRR. In addition, iron, copper and zinc,whose high concentration can negatively affect wine quality, were signicantly higher in the IRR treat-ment. The results highlighted that the absence of irrigation did not determine decreases in grape quality.Such data can be of primary importance in environments where water availability is by far the mostimportant limiting factor for plant growth.

    2012 Elsevier GmbH. All rights reserved.

    n

    avonoids, anthocyanins are pigmented compounds for the dark blue coloration of ripened grape berries.cated in the vacuoles of the hypodermal cells of berryeaf epidermis (Clarke and Bakker, 2004; Conde et al.,buti et al., 2007; Ristic et al., 2007). Flavonols, another

    ns: An, net photosynthesis; E, transpiration; ETc, cultural evapotran- reference evapotranspiration; Fv/Fm, maximum quantum yield of PSIIy; gs, stomatic conductance; IRR, irrigated plants; NIRR, non-irrigatedhotosynthetic photon ux density; SSC, soluble solid content; VPD,or pressure decit; PSII , effective quantum yield of PSII; w, steml.ding author at: Dipartimento di Scienze dei Sistemi Colturali, Forestalie Universit degli Studi della Basilicata Viale dellAteneo Lucano n. 10,, Italy. Tel.: +39 0971 206228; fax: +39 0971 204307.ress: adriano.sofo@unibas.it (A. Sofo).

    class of avonoids, are colorless berry skin constituents generallyconsidered to act as UV protectants and free-radical scavengers, andto contribute to wine color as anthocyanin co-pigments (Downeyet al., 2006; Terrier et al., 2009). The quantity and composition ofanthocyanins and avonols greatly depend on genetic factors (Bosset al., 1996), but their amount can be affected by environmentalfactors and cultural practices (Downey et al., 2006). In grapevine,water availability alters fruit chemical composition both directly,through the activation of specic metabolic pathways (Castellarinet al., 2007a,b), or indirectly through the control of berry size(Roby and Matthews, 2004; Roby et al., 2004) and other plantbiochemical and physiological processes (Conde et al., 2007). Atthe same time, grapevine anthocyanin and avonol content alsodepend on berry growth and berry skin characteristics, such asthickness and total surface per unit of volume, and the relativeproportion with respect to seeds and pulp, which is turn affectedby berry size (Hardie et al., 1996; Roby et al., 2004; Cadot et al.,2011).

    see front matter 2012 Elsevier GmbH. All rights reserved.rg/10.1016/j.jplph.2012.03.007 di Scienze dei Sistemi Colturali, Forestali e dell Ambiente, Universit degli Studi della B di Chimica, Universit degli Studi della Basilicata, Viale dellAteneo Lucano 10, I-85100

    di Chimica Farmaceutica e Tossicologica, Universit di Napoli Federico II, Via D. Monte

    e i n f o a b s t r a c tr .de / jp lph

    nd non-irrigated grapevine

    anfrab, Mauro De Niscoc, Antonio Scopaa

    ta, Via dellAteneo Lucano 10, I-85100 Potenza, Italyza, Italy9, I-80131 Napoli, Italy

  • 1024 A. Sofo et al. / Journal of Plant Physiology 169 (2012) 1023 1031

    Other than phenolics, grape and wine chemistry at the elemen-tal level (ionomic), including the content of all mineral nutrientsand trace elements, are poorly studied. All of the inorganic cationsare naturally present (not from winemaking equipment containingalloys nor ftions in graand copper(Ribreau-Gof the grapalso to the compositio

    Based ongate anthocberries of Aety of soutworldwide (1) to evaluand physiolpolyphenolthe absencevide useful iprocesses a

    Materials a

    Experimenta

    The expthe harvestgrafted on 1Marina (42

    is a climaticmation of 231 Octoberten rows oferal cordoncharacterizbetween thof 4000 vine

    Half of t(from the eaequal to 10per each ofwhereas thwas calculatranspiratiothe culturalfor grapevinirrigation sthan 0.8 Mume was oby two drirological vawithin 50 mature, rainfataken throurecorded atLeaf-to-air to Goudrian

    Plant water

    The planimental perstem water

    the row, where microclimatic conditions and soil physic-chemicalcharacteristics were similar, were chosen. The values of w weremeasured around midday on 5 fully expanded leaves and well-lightened selected from each plant on fruiting shoots situated in

    diano., Cves

    least et a

    eachents cencd frof th

    5 Ahesis

    2 wurce

    was70 techuoreS1 g moed wting lcencical ttedd lea

    lightrk (Flighttry iriablh lea

    Fv/Fm qua

    nd be

    harveld pd totoot wlantberreigh

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    ries perryter. Cean e, su

    ratiod aserry ted fzed ws, puSC) ual rrom fertilizers and pesticides) at non-toxic concentra-pe berries and wine, and some of them, such as iron, play a major role in winemaking and wine qualityayon et al., 2006). Furthermore, the metal compositione berry is of concern not only to the viticulturist, butoenologist due to the direct impact on juice and mustn, which in turn affect wine quality.

    this accepted knowledge, it was of interest to investi-yanin, avonol and metal composition within the grapeglianico, a high-quality and late-ripening grape vari-hern Italy, whose premium red wine is appreciated(Gambuti et al., 2007). The aims of this research wereate the effect of irrigation management on plant yieldogical status, and on berry morphologic characteristics,

    and metal composition, and (2) to establish whether of irrigation can affect grape quality. This could pro-nformation for improving vine cultivation, winemakingnd nal product quality.

    nd methods

    l site and plant material

    eriment was carried out in 2008, from bud break to, in a 5-year-old vineyard (cv. Aglianico clone VCR11103 Paulsen) sited on a clay-lam soil in Montegiordano02N, 1635E; Southern Italy). According to Winkler, it

    region 5, classied as very hot, with a thermic sum-603 C above the threshold of 10 C between 1 April and. The experimental plot, of about 0.30 ha, consisted of

    spur-pruned vines to a permanent horizontal unilat-. Each vine, decked at 0.60 m above the ground, wased by about 8 spurs of 23 buds each. The distancee vines was of 2.5 m 1.0 m, with a nal plant densitys ha1. Rows were north-south oriented.he plants (IRR) were irrigated from 9 June to 1 Augustrly stages of fruit set to veraison) using a water amount0% of cultural evapotranspiration (ETc) (24 L per plant

    ten irrigation turn at approximately 5-day intervals),e other half were not irrigated (NIRR). The value of ETcted using ETo Kc, where ETo is the reference evapo-n calculated according to Hargreaves method, and Kc is

    coefcient during the experimental period, equal to 0.6e, according to Allen et al. (1996). In the watered plot,

    tarted when the stem water potential ( w) was lowerPa and ended at veraison. The seasonal irrigation vol-

    f 960 m3 ha1 (240 L plant1). Each vine was irrigatedp emitter per plant discharging 4 L h1 each. Meteo-riables were monitored by a weather station placed

    of the experimental plot. Measurements of temper-ll, and photosynthetic photon ux density (PPFD) wereghout the experimental period. The values of PPFD were

    1-h intervals, and daily integrated values were logged.vapor pressure decit (VPD) was calculated according

    and Van Laar (1994).

    status, gas exchange and chlorophyll uoresc