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BC and Me – 1980 to 2003• 1980: First Meeting –NMR meeting• 1984: Joined Brit’s lab• 1984-87: Animal Models– Open Chest Animals: dog and cat
• Volume & pressure overload; ischemia; hypoxia
– Closed Chest animals: dog• Volume & pressure overload; cardiomyopathy; heart
failure; ischemia
– Other intact organs: Skeletal Muscle; liver, brain• Increase work load; ischemia
• 1987-2003: Isolated organs, cells & mitochondria
Multiple Technologies
• NMR: 31P, 23Na; 1H; 13C; 19F• NADH Fluorescence• NIR Spectroscopy• Other physiological measurements– Piezoelectric crystals: Mechanical function– Laser Doppler: flow in blood vessels; tissue perfusion– Tissue electrodes: Cae2+, Ke+, Nae+– ECG, EEG– BP, HR, LVP
World Wide Colleagues• British Connection: John Haselgrove• Chinese Connection: Daning Zhang; DJ Wang• French Connection: Bertrand Autlier; Suzanne Wehrli• German Connection: Manfred Prammer• Hungarian Connection: Laci Ligeti; Tomas Ivanics; Aristied Kovach: Kati Megyeri; Laci Deszi• Indian Connection: Hari Subramanian; Ramin Mitra• Israeli Connection: Avraham Mayevsky; Myer Shinnar• Japanese Connection: Shoko Nioka; K Niibori• Netherland Connection: Hans van Beek• Polish Connection: Maria Erecinska; Krzysztof Wroblewski • Russian Connection: Andre Savchenko• Ukrainian Connection: Nicola Doliba; Natalya Doliba; Andriy Babsky• Uruguayan Connection: Juan Crestanello• US Connection: too numerous to list• The World: too numerous to list
NMR Spectroscopy – Multiple collaborations
• Intact in vivo brain• Closed Chest Animals: Dog; Cat• Open Chest animals: Dog; Cat• Perfused Heart• Superfused Cardiomyocytes; hepatocytes• Superfused Mitochondria – heart, liver
Optical Spectroscopy & Electrode System
• NADH Fluorescence– Heart – Brain
• NIR– Oxygenation
• Multiple electrode system– Ca2+– K+– Na+
Multiparameter Analysis: Multi-channel Light Guide; Electrodes (Cae+, Ke+, Nae+; Laser Doppler
Tissue Flow; CA flow Probe