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Basic science as it applies to forensic analysis
Rebecca Hailey, Florida State College - Jacksonville Chemistry
Yousef Hijji, Morgan State U ChemistryRachel Mohr, WVU Forensic Science
Martin Overly, WVU Forensic ScienceElizabeth Pollock, Richard Stockton College of NJ,
ChemistryJacqueline Speir, WVU Forensic Science
Shuo Wei, WVU Biology
Target audience
• Introductory biology• Second semester general chemistry• Second semester general, organic, biological
chemistry• Sophomore level forensic science students
Learning goals and outcomes
Learning Goals• Students will learn about
enzyme binding and specificity
• Will understand the role of inhibitors
• Apply knowledge to novel situation
Learning Outcomes• Will be able to predict reactions
between given enzymes and substrates
• Will be able to interpret enzyme assay test results
• Will be able to predict the consequences of the addition of an inhibitor
• Will be able to relate the theoretical understanding of enzymes to real life applications
What do we need to know to solve this case?
What do we know about saliva?
How is it different from other body fluids?
Which of the following components of saliva should we target for detection?
A. WaterB. Salts C. CarbohydratesD. Food particlesE. Salivary enzymes = amylase
Demonstration of Phadebas test for the presence of amylase
Which of the following is a positive test for saliva?
How enzymes workIdentify the components a-d
enzyme substrateactive site product
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
substrateactive site product
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
Substrate (e.g. starch)
active site product
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
substrate (e.g. starch)
active site
product
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
substrate (e.g. starch)
active site
product
animation
Which one(s) of the following contains molecules which can serve as
substrates for amylase?
A. ButterB. Dietary fiber/celluloseC. Jolly RanchersD. Egg whitesE. Potato chips
Has the suspect been cleared, or looking increasingly guilty?
Pharmaceutical case study
You are a scientist at a pharmaceutical company and you are tasked with coming up with a pill that will mimic the Atkins diet (but, still allowing dieters to consume carbohydrates). What do you need to know to do your job?