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Badre & Cooper
Reduced Calcium Channel Function in the Drosophila cacTS2 mutant on Vision, Olfaction and
Regulation of the Heart
Univ of Kentucky, Department of Biology
Previous Work
– Drosophila mutants:• The mutated gene cacTS2, is a temperature sensitive
paralytic allele of Drosophila calcium channel subunit gene (Brooks et al., 2003)
– Known Effects:• Decrease synaptic transmission at the NMJ (Kawasaki et
al., 2004).
• Role in male courtship behavior (Chan et al., 2002)
• Role in nerve development (Xing et al. 2005).
•What are the Cac mutants?
Learning Test - Larvae
x 10 Before
Vision - Learning Part
After
Light + FRU Dark + QUI
x 10 Before
Olfaction - Learning Part
After
OCT + FRU AM + QUI
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Gerber et al., 2003
Hendel et al., 2005
Results Learning Vision - 33°C
Change in light preference due to learning test
% P
refe
renc
e to
Lig
ht S
ide
0
20
40
60
80
100
CSCac
AfterBefore
-24 animals
-4 trials
0
10
20
30
40
50
60
70
80
90
100
CSCol 9: 2.63 Col 9: 48.87 Col 9: 60.5 Col 11 Col 11: 50.83 Col 11: 49.67
Change in odor preference due to learning test
% P
refe
renc
e to
1-o
ctan
ol
AfterBefore
Results Learning Olfaction - 33°C
-40 animals
-2 trials
T-maze - Adults
o_
o_
o_
o_
o_
o_ o_
o_
o_
o_
o_
o_
9.5 cm 20.1 cm 9.5 cm
2.3 cm 2.3 cm 2.3 cm 2.3 cm
1.2 cm
Results – T-maze Vision 1 – Room Temp
n = 5
Light preference in CS and Cac adult flies at room temp
Side Preference
% o
f fli
es
0
20
40
60
80
CS
Cac
Light Dark Center
0
20
40
60
80
CSCac
Light preference in CS and Cac adult flies at 33°C
Side Preference
% o
f flie
s
Light Dark Center
*
Results – T-maze Vision 1 – 33°C
n = 5
Results – T-maze Vision 2 – Room Temp
n = 5
0
10
20
30
40
50
CSCac
Light preference in CS and Cac adult flies at room temp
Side Preference
% o
f flie
s
Light Dark Center
0
10
20
30
40
50
CSCac
Light preference in CS and Cac adult flies at 33°C
Side Preference
% o
f fli
es
Light Dark Center
Results – T-maze Vision 2 – 33° C
**
n = 5
Results – T-maze Olfaction – Room Temp
0
10
20
30
40
50
60
Col 7: 19.44 Col 7: 47.65 Col 7: 32.91 Col 9 Col 9: 17.06 Col 9: 46.76 Col 9: 36.18
Olfactory preference in CS and Cac adult flies at room temp
Side Preference
% o
f fli
es
Ethyl Acetate Water Center
n = 5
0
10
20
30
40
50
60
CSCac
Olfactory preference in CS and Cac adult flies at 33°C
Side Preference
% o
f fli
es
Ethyl Acetate Water Center
Results – T-maze Olfaction – 33°C
*
*
n = 5
Results Cardiac Activity - Larvae
% D
iffer
en
ce in
he
art
ra
te
-20
-15
-10
-5
0
CacCS
% Difference in Heart Rate after 30 min at 33°C for Cac and CS flies
Cac CS
n.s.
n = 5
Summary
• Cac mutants larvae have decreased abilities to learn in both vision and olfaction at higher temperatures.
• Cac mutants adults have decreased perception in both vision and olfaction at higher temperatures.
• Cac mutants larvae do not have significantly altered heart rate at higher temperature.
Acknowledgments: Arnold and Mabel Beckman Foundation, Ribble Fellowship, University of Kentucky
Undergraduate Research Program, Society for the Promotion of Undergraduate Research.
Future Directions
• I am currently working on electroretinograms and electro-olfactorygrams .
