BacterialBacterial Culture MediaCulture Media

C lt di tifi i ll Culture media : an artificially prepared substance containing prepared substance containing various microbial nutritional requirements at optimum pH which will allow the growth of which will allow the growth of most microorganisms. gIn order to cultivate these M.O. in large numbers to study them.

Types of media according to consistency:Types of media according to consistency:1. Liquid (broth)----- (0%) No agarq ( ) ( ) g2. Semi-solid ----- 0.5% agar3 S lid 2 3% 3. Solid ----- 2-3% agar

Solid media

Liquid media (Broth)

uninoculatedInoculated (turbid)

Use of broth in biochemical tests

B th diBroth media

+ve -ve

A-Simple media.(nutrient agar and broth, pepton water) B-Special media. 1. Enriched media. (blood, chocolate agar) ( , g )2. Selective media.(MacConkey, TCBS, SS and LJ)3. Differential media.(MacConkey, S-S, blood agar)3. Differential media.(MacConkey, S S, blood agar)4. Indicator media.(Mannitol Salt agar, MacConkey, EMB)EMB)5. Transport media.(Thioglycollate broth, Stuart’s medium)

Classification according to oxygenClassification according to oxygen requirement:

Aerobic media ( i l ti d di )-Aerobic media (previously mentioned media).

-Anaerobic media:Robertson’s cooked meat medium (RCM) Thioglycollate liquid mediumThioglycollate liquid medium.

Nutrient agarNutrient agarNutrient agarNutrient agarDifferent staphylococcal species on nutrient agar

Blood agar

Lactose fermenter Non-Lactose fermenter

Lactose Lactose fermenters as

l dpink colored colonies on MacConkey

mediummedium

Neutral red( i di )(as indicator)

Salmonella species Shigella

ChromagarChromagarChromagarChromagar

Vibrio cholerae

Mannitol Salt Agarg(Phenol red)

i l fMannitol fermenter

EMB (Eosin EMB (Eosin methylenemethylene blue) blue) ( t lli h l )( t lli h l )(green metallic sheen colony)(green metallic sheen colony)

Robertson’s cooked meat medium(RCM)