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The original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic™ System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.BacMagic™-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins. The BacMagic™-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.
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EMD Chemicals Inc.480 South Democrat Road Gibbstown, NJ 08027
BacMagic™ SystemsplEx/Bac™ Expression Vectors
Using insect cells for recombinant protein expression is not a new process. However, this process has traditionally been tedious and labor intensive. In recent years there have been advancements in insect cell mediated expression making this a useful tool for recombinant protein expression. We continue to bring you new solutions for your insect expression needs.
For more information or to orderNovagen products, contact EMD Chemicals.
OrdersPhone 800 854 3417Fax 800 776 0999
Technical SupportPhone 800 628 8470E-mail [email protected]
Visit our websitewww.emdbiosciences.com
You can also order Novagen products throughour distribution partner, VWR InternationalPhone 800 932 5000Web www.vwr.com
LT 10003558 10/09
BacMagic™, pIEx/Bac™, pBAC™ and TriEx™, GeneJuice®, InsectDirect®, BacVector®, GST•Tag™ and Novagen® are trademarks of EMD Chemicals Inc. or its affiliated companies.
Strep•Tag® is a registered trademark of Institut fuer Bioanalytik GmbH.
Product prices and availability are subject to change. Products are warranted only to conform to the specifications set forth on their label/packaging and/or certificate of analysis at the time of shipment or for the expressly stated duration. NO OTHER WARRANTY WHETHER EXPRESS, IMPLIED OR BY OPERATION OF LAW IS GRANTED. The products are intended for research purposes only and are not to be used for drug or diagnostic purposes, or for human use. The products may not be resold or used to manufacture commercial products without the prior written approval of EMD Chemicals. EMD makes no warranties of any kind for any technical advice provided by EMD, or for any results occurring as a result of the application of such advice. Product users must review the full instruction set included with the product prior to use. All sales are subject to EMD Chemical's complete Terms and Conditions of Sale.
PRESORT STANDARDU.S. POSTAGE
PAID MADISON, WI
PERMIT No. 339
Insect.indd 12-1 10/9/2009 3:08:27 PM
BacMagic™ SystemThe original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic™ System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.
BacMagic™-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins. The BacMagic™-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.
pIEx/Bac™ Expression VectorspIEx/Bac™ dual purpose vectors are the perfect combination with BacMagic™ System for baculovirus infection and expression. Because the pIEx/Bac™ vectors are compatible with both plasmid-mediated and baculovirus expression, it is easy to use a single construct in both expression systems for HT screening and optimized high-yield protein expression.
Homologous recombination schemes of the BacMagic™-3 System
∆chiA
Gene ORF1629
Recombinant virus
BacMagic™-3 DNA
∆chiA
lef2 Bac
Gene ORF1629
lef2
lef2
1629
Transfer plasmid
∆v-cath
∆v-cath
∆p10
∆p10
Day 1 Day 5 Day 8 - 10 Days 11 - 13
Cotransfect insect cells with recombinant transfer plasmid
plus an AcNPV BacMagic™ DNA
Harvest recombinant baculovirus; screen for expression; amplify viral
stock (titer stock, optional)
Infect insect cells and express protein
Harvest cells and proceed with purification
BacMagic™ System: No Plaque Purification Method
Product Size Cat. No. Product Size Cat. No.
BacMagic™ DNA Kit 5 rxn 71545-3 BacMagic™ Transfection Kit 5 rxn 71546-3
BacMagic™-2 DNA Kit 5 rxn 72157-3 BacMagic™ -2Transfection Kit 5 rxn 72158-3
BacMagic™-3 DNA Kit 5 rxn 72350-3 BacMagic™-3 Transfection Kit 5 rxn 72351-3
Ordering Information
• Faster baculovirus production without tedious plaque purification• Compatible with pIEx/Bac™, pBAC™, pTriEx™, and other transfer plasmids using the lef2/603 and ORF1629 recombination sites• Deletion of chitinase to maximize secreted and membrane targeted protein production• Includes Insect GeneJuice® Transfection Reagent for high efficiency transfection
New pIEx/Bac™-4 and –5 vectors encode an N-terminal GST•Tag™ sequence (Table 1). The GST•Tag™ affinity tag is popular for insect cell expression because it may improve solubility for some proteins.
Table 1. plEx/Bac vector features
Tb, Thrombin; Ek, enterokinase; 3C, HRV 3C protease
Vector N-Terminal Fusion Tag C-Terminal Fusion Tag Protease
Cleavage SitesVector size (bp)
pIEx/Bac™-1 Strep•Tag® II His •Tag® Ek 6796
pIEx/Bac™-1 Ek/LIC Strep•Tag ® II His •Tag® Ek 6751
pIEx/Bac™-3 His •Tag® Strep•Tag® II 3C/Tb 6802
pIEx/Bac™-3 3C/LIC His •Tag® Strep•Tag® II 3C/Tb 6763
pIEx/Bac™-4 GST •Tag™ His •Tag® Ek 7496
pIEx/Bac™-4 Ek/LIC GST •Tag™ His •Tag® Ek 7451
pIEx/Bac™-5 GST •Tag™ Strep•Tag® II 3C/Tb 7499
pIEx/Bac™-5 3C/LIC GST •Tag™ Strep•Tag® II 3C/Tb 7460
Product Size Cat. No. Product Size Cat. No.
pIEx/Bac™-1 DNA 20 µg 71724-3 pIEx/Bac™-1 Ek/LIC Vector Kit 20 rxn 71729-3
pIEx/Bac™-3 DNA 20 µg 71726-3 pIEx/Bac™-3 3C/LIC Vector Kit 20 rxn 71731-3
pIEx/Bac™-4 DNA 20 µg 71727-3 pIEx/Bac™-4 Ek/LIC Vector Kit 20 rxn 71732-3
pIEx/Bac™-5 DNA 20 µg 71728-3 pIEx/Bac™-5 3C/LIC Vector Kit 20 rxn 71733-3
Ordering Information
Complete vector sequences and maps are available at: www.emdbiosciences.com/vector
• AcNPV hr5/ie1 promoter/enhancer for plasmid mediated and early baculovirus expression
• AcNPV p10 very late promoter for late baculovirus expression
• N-terminal and C-terminal fusion tags for dual purification strategies
• Available as LIC Vector Kits for efficient, flexible ligation- independent cloning
Ideal for use in the InsectDirect® System for HT screening
Ideal for use in the BacMagic or BacVector® Systems for robust protein expression
Facilitates purification of full-length proteins
Speeds experimental results
Insect.indd 2-3 10/9/2009 3:08:27 PM
BacMagic™ SystemThe original BacMagic™ System allows you to generate recombinant baculoviruses without the time-consuming plaque purification process. The next generation of the BacMagic™ System has the added advantage of improved quality and yield for most target proteins, through the deletion of additional non-essential genes.
BacMagic™-2 DNA deletes chiA and v-cath genes, resulting in significantly improved quality and yield for most target proteins. The BacMagic™-3 DNA combines the deletion chiA and v-cath with the additional deletion of three more non-essential virus genes, p10, p74 and p26, for more efficient expression.
pIEx/Bac™ Expression VectorspIEx/Bac™ dual purpose vectors are the perfect combination with BacMagic™ System for baculovirus infection and expression. Because the pIEx/Bac™ vectors are compatible with both plasmid-mediated and baculovirus expression, it is easy to use a single construct in both expression systems for HT screening and optimized high-yield protein expression.
Homologous recombination schemes of the BacMagic™-3 System
∆chiA
Gene ORF1629
Recombinant virus
BacMagic™-3 DNA
∆chiA
lef2 Bac
Gene ORF1629
lef2
lef2
1629
Transfer plasmid
∆v-cath
∆v-cath
∆p10
∆p10
Day 1 Day 5 Day 8 - 10 Days 11 - 13
Cotransfect insect cells with recombinant transfer plasmid
plus an AcNPV BacMagic™ DNA
Harvest recombinant baculovirus; screen for expression; amplify viral
stock (titer stock, optional)
Infect insect cells and express protein
Harvest cells and proceed with purification
BacMagic™ System: No Plaque Purification Method
Product Size Cat. No. Product Size Cat. No.
BacMagic™ DNA Kit 5 rxn 71545-3 BacMagic™ Transfection Kit 5 rxn 71546-3
BacMagic™-2 DNA Kit 5 rxn 72157-3 BacMagic™ -2Transfection Kit 5 rxn 72158-3
BacMagic™-3 DNA Kit 5 rxn 72350-3 BacMagic™-3 Transfection Kit 5 rxn 72351-3
Ordering Information
• Faster baculovirus production without tedious plaque purification• Compatible with pIEx/Bac™, pBAC™, pTriEx™, and other transfer plasmids using the lef2/603 and ORF1629 recombination sites• Deletion of chitinase to maximize secreted and membrane targeted protein production• Includes Insect GeneJuice® Transfection Reagent for high efficiency transfection
New pIEx/Bac™-4 and –5 vectors encode an N-terminal GST•Tag™ sequence (Table 1). The GST•Tag™ affinity tag is popular for insect cell expression because it may improve solubility for some proteins.
Table 1. plEx/Bac vector features
Tb, Thrombin; Ek, enterokinase; 3C, HRV 3C protease
Vector N-Terminal Fusion Tag C-Terminal Fusion Tag Protease
Cleavage SitesVector size (bp)
pIEx/Bac™-1 Strep•Tag® II His •Tag® Ek 6796
pIEx/Bac™-1 Ek/LIC Strep•Tag ® II His •Tag® Ek 6751
pIEx/Bac™-3 His •Tag® Strep•Tag® II 3C/Tb 6802
pIEx/Bac™-3 3C/LIC His •Tag® Strep•Tag® II 3C/Tb 6763
pIEx/Bac™-4 GST •Tag™ His •Tag® Ek 7496
pIEx/Bac™-4 Ek/LIC GST •Tag™ His •Tag® Ek 7451
pIEx/Bac™-5 GST •Tag™ Strep•Tag® II 3C/Tb 7499
pIEx/Bac™-5 3C/LIC GST •Tag™ Strep•Tag® II 3C/Tb 7460
Product Size Cat. No. Product Size Cat. No.
pIEx/Bac™-1 DNA 20 µg 71724-3 pIEx/Bac™-1 Ek/LIC Vector Kit 20 rxn 71729-3
pIEx/Bac™-3 DNA 20 µg 71726-3 pIEx/Bac™-3 3C/LIC Vector Kit 20 rxn 71731-3
pIEx/Bac™-4 DNA 20 µg 71727-3 pIEx/Bac™-4 Ek/LIC Vector Kit 20 rxn 71732-3
pIEx/Bac™-5 DNA 20 µg 71728-3 pIEx/Bac™-5 3C/LIC Vector Kit 20 rxn 71733-3
Ordering Information
Complete vector sequences and maps are available at: www.emdbiosciences.com/vector
• AcNPV hr5/ie1 promoter/enhancer for plasmid mediated and early baculovirus expression
• AcNPV p10 very late promoter for late baculovirus expression
• N-terminal and C-terminal fusion tags for dual purification strategies
• Available as LIC Vector Kits for efficient, flexible ligation- independent cloning
Ideal for use in the InsectDirect® System for HT screening
Ideal for use in the BacMagic or BacVector® Systems for robust protein expression
Facilitates purification of full-length proteins
Speeds experimental results
Insect.indd 2-3 10/9/2009 3:08:27 PM
EMD Chemicals Inc.480 South Democrat Road Gibbstown, NJ 08027
BacMagic™ SystemsplEx/Bac™ Expression Vectors
Using insect cells for recombinant protein expression is not a new process. However, this process has traditionally been tedious and labor intensive. In recent years there have been advancements in insect cell mediated expression making this a useful tool for recombinant protein expression. We continue to bring you new solutions for your insect expression needs.
For more information or to orderNovagen products, contact EMD Chemicals.
OrdersPhone 800 854 3417Fax 800 776 0999
Technical SupportPhone 800 628 8470E-mail [email protected]
Visit our websitewww.emdbiosciences.com
You can also order Novagen products throughour distribution partner, VWR InternationalPhone 800 932 5000Web www.vwr.com
LT 10003558 10/09
BacMagic™, pIEx/Bac™, pBAC™ and TriEx™, GeneJuice®, InsectDirect®, BacVector®, GST•Tag™ and Novagen® are trademarks of EMD Chemicals Inc. or its affiliated companies.
Strep•Tag® is a registered trademark of Institut fuer Bioanalytik GmbH.
Product prices and availability are subject to change. Products are warranted only to conform to the specifications set forth on their label/packaging and/or certificate of analysis at the time of shipment or for the expressly stated duration. NO OTHER WARRANTY WHETHER EXPRESS, IMPLIED OR BY OPERATION OF LAW IS GRANTED. The products are intended for research purposes only and are not to be used for drug or diagnostic purposes, or for human use. The products may not be resold or used to manufacture commercial products without the prior written approval of EMD Chemicals. EMD makes no warranties of any kind for any technical advice provided by EMD, or for any results occurring as a result of the application of such advice. Product users must review the full instruction set included with the product prior to use. All sales are subject to EMD Chemical's complete Terms and Conditions of Sale.
PRESORT STANDARDU.S. POSTAGE
PAID MADISON, WI
PERMIT No. 339
Insect.indd 12-1 10/9/2009 3:08:27 PM
