Bru

ker

Nan

o S

urfa

ces

Div

isio

n is

con

tinua

lly im

prov

ing

its p

rodu

cts

and

rese

rves

the

rig

ht t

o ch

ange

spe

cific

atio

ns w

ithou

t no

tice.

©20

14 B

ruke

r C

orpo

ratio

n.

All

right

s re

serv

ed. U

ltim

a an

d P

rairi

e V

iew

are

tra

dem

arks

of

Bru

ker

Cor

pora

tion.

All

othe

r tr

adem

arks

are

the

pro

pert

y of

the

ir re

spec

tive

com

pani

es. B

2001

, Rev

. A0

Ultima Multiphoton Microscope Systems

Leading Advances in Life Science Discovery with Fast, Deep Multiphoton Microscopy

Fluorescence MicroscopyInnovation with Integrity

Ultima Multiphoton Microscope Systems

At the forefront of scientific discoveries for more than ten years,

Ultima Multiphoton Microscope Systems are the direct result of

decades of laboratory experience and close collaboration with

leading biological scientists around the world. The involvement of

these life science experts has led to a host of unique capabilities

and features found only on Ultima systems, such as their modular

setup, incorporated photostimulation light path, and Prairie View

software. Only Ultima delivers the combination of flexibility,

resolution, imaging depth and speed, simultaneous imaging and

photostimulation, and imaging integrated with external stimuli and

electrophysiology recording.

GFP labeled in M-line female mouse, max projection, 900 μm.

Thy-1 YFP in retinal ganglion cells.

Ultima’s Performance Advantage � Collaboration with top researchers provides unparalleled functionality

� Field-proven technology provides unmatched reliability

� Open architecture for flexibility and upgradability

From the introduction of the first commercial system to allow simultaneous imaging and uncaging in 2003, to the latest intravital system with its resonant scanner and simultaneous optogenetic stimulation, Ultima multiphoton microscopes have grown into the systems of choice for cutting-edge research around the world.

Robust and Reliable Technology

Ultima systems provide reliable operation in high-use environments from the moment installation is complete because our manufacturing and installation processes are based on more than ten decades of combined staff experience in design, manufacturing, quality assurance, and product installation. You can begin research the same day your Ultima system is installed.

Refined and nimble enough for the most precise and complex protocols, Ultima systems are workhorses that will provide years of consistent, trouble-free service. Simply put, we make the technology work. Bruker will continue to innovate to keep your system at the cutting-edge of the technology far into the future.

Flexibility to Meet Specific Research Needs

The flexible architecture of the Ultima systems provides an extensible platform that can be upgraded over time as research needs evolve. Open access to optical paths and electronics plus powerful software enable user customization of the systems, as well as integration into complex research systems that incorporate other electronics and computational platforms.

The Ultima is available in three configurations. Ultima IntraVital is optimized for in vivo applications utilizing live animals across a range of model systems. Ultima In Vitro incorporates a traditional optical microscope in either an upright or inverted configuration, and it is optimized for tissue slices, tissue explants, and cell culture applications. Ultima Investigator is an entry-level multiphoton imaging system available in an upright configuration.

A Legendary Design Keeps Getting Better

Montage image of neural network.

In vivo image of tumor explant, CFP-Tumor cellls-MMTV-Cre GFP-macrophage – C-fms promoter.

Designed specifically for intravital studies, this system provides the imaging depth and resolution required for the most demanding live-animal research applications. The Ultima IntraVital is the result of a continuous innovation process, and offers the following advances:

� An upright light path with extended clearance under the objective

� Precise triggering for integration with external behavioral trial and electrophysiology systems

� High-speed volumetric imaging options

� Optogenetics options

� A moving X,Y platform option for simplified positioning of the objective with respect to a stationary specimen

� Optionally convertible to a standard upright microscope configuration with bridge stage and transmitted light components

Ultima Intravital’s scanner is mounted on a customizable upright frame, allowing room to accommodate a wide range of specimens such as primates, small mammals, and invertebrate models, as well as tissue slices, excised tissue, and cell cultures (with the bridge stage option).

Ultima Intravital excels at applications such as neuronal signaling, cell migration, structural changes, tissue structure, protein and vesicle transport, metabolic activity, and intracellular ion dynamics. It is optimized to meet the rigorous demands of complex, advanced research.

Ultima IntraVital

Cortical neuron firing as measured by calcium changes using GCaMP as indicator.

Dendritic spine modification in juvenile mouse brain after exposure to enhanced environment.

Pre Post

2 hr EE

5 μm

Ultima In Vitro

The Ultima In Vitro Multiphoton Microscope System is the platform on which leading scientists have conducted breakthrough experiments in brain-slice studies related to neural signaling and neural network mapping. Ultima In Vitro provides simultaneous imaging and electrophysiology, plus uncaging and photostimulation for the complete stimulus and recording environment required for research by today’s neuroscientist.

� Available on upright or inverted optical microscopes

� Precise triggering for integration with electrophysiology systems

� High-speed volumetric imaging options

� Optogenetics options

Typical Ultima In Vitro applications include neuronal signaling, tissue structural changes, protein transport, and intracellular ion dynamics. Ultima In Vitro’s proven technology excels at brain-slice studies, excised tissue examinations, and cell cultures.

Striatal medium spiny neuron (MSN) from a transgenic mouse (BAC D2).

Hippocampal pyramidal neuron and voltage recordings after uncaging.

The true power of the Ultima systems lies in their ability to be custom configured. An array of options is available to extend the systems to meet your particular research needs:

The Photostimulation Path provides the capability for simultaneous photostimulation or uncaging with imaging. It works with fast-pulsed IR, UV, or visible laser inputs, and accommodates multiple scan types. It also will precisely synchronize with imaging and external triggers.

The Orbital Nosepiece provides off-axis imaging (objective tilt and rotation) to access specimen areas of interest not accessible through the vertical axis, and ensures a proper angle to minimize scatter in tissue with highly oriented cells. The Orbital Nosepiece is fully motorized, and tilt and rotation are under software control. When oriented off the vertical axis, software drives the objective in X,Y orthogonal to objective axis and Z parallel to the objective axis. The unique fiber-optic-based collection optics in the Orbital Nosepiece provide high efficiency signal collection, even with lower power, large back-aperture objectives.

Dual Wavelength Imaging enables routing of two lasers into the imaging path of the microscope, providing the capability to use multiple probes without laser tuning during imaging. Scanning can be performed simultaneously or in a line-interlaced mode.

The Bridge Stage option includes a low-profile, motorized stage with a specimen platform. Easily inserted or removed, the Bridge Stage converts the Ultima Intravital into a slice rig. It also is compatible with Bruker substage detectors.

The Full-Field Optical Input/Output module includes a camera port with variable zoom and an optical input port for full-field photoactivation or a stimulus projector.

Fluorescence Lifetime Imaging (FLIM) provides additional electronics for FLIM detection and FLIM acquisition with all raster galvo scan modes, ROIs, and T-series and Z-series acquisitions.

Substage Detectors that include one or two detectors for collection of transmitted fluorescence signals and increased efficiency for dim signals are available on all systems. The detectors also provide Second Harmonic Generation signal collection.

Unmatched Options for Extensibility

Orbital nosepiece.

Dual wavelength imaging in lymph node explant.

Superior Options for High-Speed Imaging

Current and future research requires high-speed, full-field, and volumetric imaging to meet the demands and growth of emerging application areas. Bruker’s Ultima offers options for increased speed, providing an unmatched ability to capture fast cellular dynamics, moving multiphoton imaging to the next level.

The Resonant Scanner provides high-speed acquisition of 512 x 512 images at 30 fps. Its unique relayed-beam path utilizes X and Y galvos for ROIs, allowing ROIs to be created using a reduced scan area in both X and Y. This provides not only faster acquisition rates but also an increase in signal-to-noise ratio. ROIs also can be panned in X and Y for precise positioning.

The Resonant Scanner can be used simultaneously with a photostimulation path either in full-frame mode or while using ROIs, allowing photostimulation to occur simultaneously with high-speed imaging.

Piezo Focus provides fast focus over a 150 µm range. Its unique calibration method allows for fast volumetric imaging using a continuous scan through Z. Bidirectional scanning in calibrated mode provides 90% duty-cycle volumetric imaging.

The Piezo Focus can be used with both standard raster scanning and with resonant scanning. When used with the Resonant Scanner, Jumping Mode provides tracking of multi-plane ion dynamics.

Ultima multiphoton microscopes give you the tools you need to take your research to the next level in brain slice studies, uncaging, optogenetics, and deep imaging into tissues. The Ultima systems’ modular setup, flexibility, superior resolution, imaging depth and speed, plus our industry-leading Prairie View software, all combine to give you the power to accelerate your research, and move it into new frontiers of discovery.

Resonant scanner imaging of neuronal signaling, indicated by changes in calcium level of layer 2/3 cells in somatosensory cortex of an awake mouse during behavioral trial. Changes in calcium level measured using GCaMP.

Ultima SpecificationsScanheadScanning Method Matched pair of 3 mm Cambridge galvonometers; upgradable to 6 mm

Scan Size Pre-set images sizes from 64 x 64 to 2048 x 2048

Field of View 60x objective: ~200 µm x 200 µm; 40x objective: ~300 µm x 300 µm; 20x objective: ~600 µm x 600 µm

Scan Speeds at 1x ZoomRaster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64; Spiral scan: 6 fps at 512 x 512, >30 fps at 64 x 64

Scan CustomizationUser-definable straight, freehand, and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 to 2048; up to 128x scan zoom; 360° of scan rotation; point scan

UncagingOptional second set of matched 3 mm galvonometers in same scanhead to provide high-precision visible or 2P laser sample ablation

High-Speed Imaging Options Resonant scanner: 30 fps at 512 x 512; >1300 fps at 512 x 8 ROI

1P Confocal Optional automated pinhole and slit confocal attachment for visible-light confocal imaging

Detectors

Upper Non-DescannedConfigurable for 1 through 4 hand-picked Hamamatsu multi-alkali PMTs; upgradable to high-sensitivity Hamamatsu GaAsP detectors

Sub-Stage Non-Descanned Single or dual hand-picked Hamamatsu multi-alkali PMT configurations; upgradable to high-sensitivity Hamamatsu GaAsP PMTs

Dodt Transmitted-light Dodt gradient contrast detector for DIC-like imaging with a Hamamatsu PMT

Transmitted Light Single Hamamatsu multi-alkali PMT for the creation of a transmitted light image

Confocal ModuleConfigurations available for 1 through 3 multi-alkali PMTs; upgradable to high-sensitivityHamamatsu GaAsP detectors

CCD Standard C2 camera port built in for standard OLM image collection

Optical Inputs2P Laser Ultima light path optimized for 2P laser input; a 2P laser with pre-compensation is recommended for use with the AOD scanner

Epifluorescence Standard epifluorescence head for fluorescence imaging

Visible Laser Confocal or point-photoactivation modules for fiber-launch laser applications

LED Full-field photoactivation with LED module

Motor ControlVariable Height X-Y Stage Manual and motor-driven fine and course movement stage with minimum 0.3 μm step size

Motorized Large Platform XY Specimen Stage with Variable Height

Minimum 0.2 μm increments, 1 μm accuracy, 6” X and 3” Y travel X-Y platform; optional motor-driven fine and course movement platform for scope with minimum 0.1 μm step size; ~35 mm travel (IntraVital)

Bruker Z-Motor Available on non-motorized microscope bases with minimum 0.2 µm step size; 30 mm travel

Bruker Z-PiezoOptional piezo device for execution of high-speed z-stack acquisitions with a travel range of 0.05 µm step size and 150 µm travel; compatible with 20x water objective

Software

Prairie View ImagingPrairie View imaging fully integrated with scanhead for easy imaging; customizable scan settings for optimization of specimen excitation; integrated control of laser power and PMT high voltage

Z-Series Easy-to-set-up Z-series with user-customizable slice number or step size

T-Series Easy creation of complex series involving Z-series and triggered images

Peripherals IntegrationWavelength and power control available for 2P and visible laser launches; can be integrated with peripherals; control options offered for several common stage types

Photoactivation User-defined points and regions for complex laser blanking

Regions of Interest User-defined regions for faster scanning capabilities

Brightness over Time Capable of collecting BOT information for user-defined regions over time and/or depth

Voltage Inputs/Outputs Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with internal devices

Bruker Nano Surfaces Division

Middleton, WI • USA Phone +1.608.662.0022 productinfo@bruker.comwww.bruker.com/nano

Bru

ker

Nan

o S

urfa

ces

Div

isio

n is

con

tinua

lly im

prov

ing

its p

rodu

cts

and

rese

rves

the

rig

ht t

o ch

ange

spe

cific

atio

ns w

ithou

t no

tice.

©20

14 B

ruke

r C

orpo

ratio

n.

All

right

s re

serv

ed. U

ltim

a an

d P

rairi

e V

iew

are

tra

dem

arks

of

Bru

ker

Cor

pora

tion.

All

othe

r tr

adem

arks

are

the

pro

pert

y of

the

ir re

spec

tive

com

pani

es. B

2001

, Rev

. A0