B. MALDI IMS Ischemic Hemisphere

A. MALDI IMS Control Hemisphere

m/z 760.6 m/z 760.6

Figure 1

300300

130

400 500 600 700 800 900 1000

1500

8x

170548.5 760.6

760.6 798.6

798.6

782.6

782.6

Figure 2

548.5

A. Total ions (Control Hippocampus)

B. Ischemic Hippocampus

Sig

nal I

nten

sity

Sig

nal I

nten

sity

m/z

m/z 760.6

m/z 760.6

15001500

B. MALDI IMS Ischemic Hemisphere

A. MALDI IMS Control Hemisphere

m/z 548.5 m/z 548.5

Figure 3

Figure 4

A. TTC Stained Rat Brain Slice

550

B. 16:0/18:1 PC[M+H]+ m/z 760.6

1400

C. 16:0/18:1 PC[M+Na]+ m/z 782.6

2300

D. 16:0/18:1 PC[M+K]+ m/z 798.6

200

100 200 300 400 500 600m/z

100

Rel

ativ

e In

tens

ity

264.3

548.5530.5

308.3252.3

282.3

B. CID (pos ion) m/z 548.5A. MALDI IMS of Rat Brain 96 hours post TBI

m/z 548.5

Figure 5

Supplemental Figure 1

100 200 300 400 500 600m/z

264.3524.5

506.6282.3252.3494.5

CID m/z 524.5

Cer 18:1/13C4-16:0

[M+H]+ -H2O

Rel

ativ

e In

tens

ity

100[Cer(d18:1/13C4-16:0)-H2O]+

400 600 800m/z

782.6760.6

577.5

723.5

524.5

564.5

ESI/MS

POPC-H+POPC-Na+

Cer

(d18

:1/1

3C

-16)

-H2O

+

Cer

(d18

:1/1

3C

-16)

+