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B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W.) Affiliated to University of Mumbai DEPARTMENT OF MICROBIOLOGY Programme: Bachelor of Science (B.Sc.) SYLLABUS FOR: F.Y.B.Sc. Programme- Bachelor of Science (B.Sc.) Choice Based Credit System (CBCS) with effect from the academic year 2018-19

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Page 1: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND

COMMERCE

(AUTONOMOUS)

KALYAN (W.)

Affiliated to University of Mumbai

DEPARTMENT OF MICROBIOLOGY

Programme: Bachelor of Science (B.Sc.)

SYLLABUS FOR:

F.Y.B.Sc.

Programme- Bachelor of Science (B.Sc.)

Choice Based Credit System (CBCS) with effect from the academic

year 2018-19

Page 2: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE

(AUTONOMOUS)

KALYAN (W)

Affiliated to University of Mumbai

Choice Based Credit System (CBCS) with effect from the academic

year 2018-19

Faculty of Science

Semester I to Semester VI

Guidelines

Syllabus Structure:

1. In F.Y. B.Sc. (CBCS) in Sem I and II, there will be 2 papers with 2 credits each

for theory and 2 credits for practical, in each semester.

2. In S.Y.B.Sc. (CBCS) in Sem III and Sem IV, there will be 3 papers with 2 credits

each for theory and 3 credits for practical, in each semester.

3. In TYBSc (CBCS) in Sem V and Sem VI, there will be 4 papers with 2.5 credits

each for theory and 1.5 credits for practicals of each paper, in each semester.

4. The Applied Courses will be Environmental sciences for T.Y.BSc.

Evaluation:

1. Core Courses: The College will conduct all the semester examinations of 100

marks per Core Course in the prescribed pattern of 40 marks of Internal

assessment and 60 marks for semester end examination. The student will have to

secure a minimum of 40% marks in internal assessment as well as semester end

examination per Core Course, for all the above Core Courses.

2. Applied Courses: The Applied Courses will be Environmental sciences for

T.Y.BSc. The College will conduct all the semester examinations of 100 marks

per applied Course in the prescribed pattern of 40 marks of Internal

assessment/Project Work and 60 marks for semester end examination/Practical

Page 3: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

examination. The student will have to secure a minimum of 40% marks in internal

assessment as well as semester end examination per Core Course, for all the above

applied courses.

3. In each semester, the student will have to submit Project/ Assignment/Journal for

Core Courses and Applied Courses in the College before appearing for the

Semester End Examination. The last date of submission of the Project will be

officially declared by the College.

4. In each semester, for Core Courses and Applied Courses, the student will have to

secure a minimum of 40% marks in aggregate and a minimum of 40% in each

component of assessment i.e. 16 out of 40 marks in Internal Evaluation and 24 out

of 60 marks in semester end examination/ Practical Examination.

Note: All other rules regarding Standard of Passing, ATKT, etc., will be as per

those decided by the Faculty of Sciences passed by the Academic Council from

time to time.

Page 4: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE

(AUTONOMOUS)

KALYAN (W.)

Affiliated to University of Mumbai

CONTENT

Programme- Bachelor of Science (B.Sc.)

Sr. No. Course Code Credits

1 F.Y.B.Sc. – Fundamentals of microbiology BUSMB101 02

2 F.Y.B.Sc. - Basic Techniques in Microbiology BUSMB102 02

3 F.Y.B.Sc. - Practicals BUSMBP-1 02

4 F.Y.B.Sc. – Basics of Microbiology BUSMB201 02

5 F.Y.B.Sc. – Exploring Microbiology BUSMB202 02

6 F.Y.B.Sc. – Practicals BUSMBP-2 02

7 S.Y.B.Sc. – Biomolecules and microbial taxanomy BUSMB301 02

8 S.Y.B.Sc. – Environmental Microbiology BUSMB302 02

9 S.Y.B.Sc. – Introduction to clinical microbiology and

basic immunology.

BUSMB303 02

10 S.Y.B.Sc. – Practicals BUSMBP- 3 03

11 S.Y.B.Sc. –Metabolism and basic analytical techniques BUSMB401 02

12 S.Y.B.Sc. – Applied Microbiology BUSMB402 02

13 S.Y.B.Sc. – Fermented Food, Food sanitation and

microbial ecology

BUSMB403 02

14 S.Y.B.Sc. - Practicals BUSMBP-4 03

15 T.Y.B.Sc. – Microbial Genetics BUSMB501 2.5

16 T.Y.B.Sc. – Medical microbiology and immunology -1 BUSMB502 2.5

17 T.Y.B.Sc. – Microbial Biochemistry-1 BUSMB503 2.5

18 T.Y.B.Sc. – Bioprocess Technology -1 BUSMB504 2.5

Page 5: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

19 T.Y.B.Sc. – Practicals BUSMBP-05 1.5

20 T.Y.B.Sc. – r-DNA Technology, Bioinformatics and

Virology

BUSMB601 2.5

21 T.Y.B.Sc. – Medical microbiology and immunology -2 BUSMB602 2.5

22 T.Y.B.Sc. - Microbial Biochemistry-2 BUSMB603 2.5

23 T.Y.B.Sc. – Bioprocess Technology -2 BUSMB604 2.5

24 T.Y.B.Sc. – Practicals BUSMBP -06 1.5

CONTENT

Programme- Bachelor of Science

Applied Course

Sr. No. Course Code Credits

1 T.Y.B.Sc.- Environmental Studies-I BUSEVS101 02

2 T.Y.B.Sc.- Environmental Studies-II BUSEVS201 02

Programme- Certificate Course

Career Oriented Additional Courses

Sr. No. Course Code Credits

1 Macromolecules 02

2 Hands on training in fermentation technology 02

Page 6: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE

(AUTONOMOUS)

KALYAN (W.)

Affiliated to University of Mumbai

Evaluation Pattern

1. INTERNAL ASSESSMENT 40 marks

1.1

1.2

1.3

One class test (Objectives/ Multiple Choice)

Assignment/ Project/ Presentation

Active participation, Overall performance

15 marks

20 marks

5 marks

2. EXTERNAL ASSESSMENT (Semester End Examination) 60 marks

N.B. 1. All questions are compulsory

2. All questions carry equal marks.

Q.1 Unit-I (with internal option)

A.

B.

C.

15 marks

Q.2 Unit-II (with internal option)

A.

B.

C.

15 marks

Q.3 Unit-III (with internal option)

A.

B.

C.

15 marks

Q.4 Unit-IV (with internal option)

A.

B.

C.

15 marks

Page 7: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

B. K. Birla College of Arts, Science and Commerce, Kalyan (W.)

Syllabus w.e.f. Academic Year, 2018-19 (CBCS)

F.Y. B.Sc Semester- I

Microbiology

COURSE CODE: BUSMB101 (2018-19) (Theory) Credits- 02

Title:- Fundamentals Of Microbiology

Sr. No. Modules/Units Lectures (45)

1 History, Introduction & Scope of Microbiology Prokaryotic Cell

Structure,

15

2 Eukaryotic Cell Structure, Biosafety In Microbiology 15

3 Macromolecules 15

COURSE CODE: BUSMB102 (2018-19) (Theory) Credits- 01

Title:- Basic Techniques in Microbiology

Sr. No. Modules/Units Lectures (45)

1 Microscopy & Staining 15

2 Control of Microorganisms 15

3 Microbial Nutrition, Cultivation, Isolation & Preservation 15

COURSE CODE: BUSMBP101 (2018-19) (Practical) Credits- 02

SECTION - I

Title:- Fundamentals of Microbiology (Practicals Based on Unit-I, II & III of BUSMB-101)

SECTION - II

Title:- Basic Techniques in Microbiology (Practicals Based on Unit-I, II & III of BUSMB-102)

Page 8: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

F.Y. B. Sc Semester- II

Microbiology

COURSE CODE: BUSMB201 (2018-19) (Theory) Credits- 02

Title:- Basics of Microbiology

Sr. No. Modules/Units Lectures (45)

1 Study of Different Groups Of Microbes-I 15

2 Study of Different Groups Of Microbes-II 15

3 Microbial Growth 15

F.Y. B. Sc Semester- II

Microbiology

COURSE CODE: BUSMB202 (2018-19) (Theory) Credits- 02

Title:- Exploring Microbiology

Sr. No. Modules/Units Lectures (45)

1 Microbial Interactions 15

2 Microbes & Human Health 15

3 Advance Techniques In Microbiology & Instrumentation 15

COURSE CODE: BUSMBP201 (2018-19) (Practical) Credits- 02

SECTION - I

Title:- Basics of Microbiology (Practicals Based on Unit-I, II & III of BUSMB-201)

SECTION - II

Title:- Exploring Microbiology (Practicals Based on Unit-I, II & III of BUSMB-202)

Page 9: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

B. K. Birla College of Arts, Science and Commerce, Kalyan (W.)

Syllabus w.e.f. Academic Year, 2018-19 (CBCS)

F.Y. B.Sc Semester- I

Microbiology

COURSE CODE: BUSMB101 (2018-19) (Theory) Credits- 02

Title:- Fundamentals of Microbiology

Sr.

No.

Modules/Units Lectures

(45)

1.

1.1

1.2

History, Introduction & Scope of Microbiology Prokaryotic Cell Structure,

a) Discovery of microorganisms

b) Conflict over spontaneous generation.

c) Golden age of microbiology-Koch Postulate, medical microbiology,

immunology

d) Development of industrial microbiology and microbial ecology

e) Scope and relevance of microbiology

f) Future of microbiology

Prokaryotic Cell Structure and functions:

a. Cell wall

b. Cell membrane

c. Components external to cell wall-capsule, slime layer, flagella, pili, fimbriae

d. Cytoplasmic matrix-inclusion bodies, magnetosomes, ribosomes, gas vesicles,

nucleoid, plasmids

e. Bacterial endospores and their formation

15

2.1

Eukaryotic Cell Structure

a. Overview of eukaryotic cell structure

b. The plasma membrane and membrane structure

c. Cytoplasmic matrix, microfilaments, intermediate filaments, and

microtubules

d. Organelles of the biosynthetic-secretory and endocytic pathways –

15

Page 10: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

2.2

endoplasmic reticulum & golgi apparatus. definitions of lysosome,

endocytosis, phagocytosis, autophagy, proteasome

e. Eukaryotic ribosomes,

f. Mitochondria

g. Chloroplasts

h. Nucleus –nuclear structure

i. External cell coverings: cilia and flagella

j. Comparison of prokaryotic and eukaryotic Cells

Biosafety In Microbiology

a. Means of laboratory infection

b. Potentially hazardous procedures

c. Responsibility

d. Risk assessment

e. Restricted access

f. Safety equipments

g. Immunization and medical records,

h. Training of personnel

i. Laboratory procedures

j. Levels of Containment

3

3.1

3.2

3.3

3.4

Macromolecules

a. Biomolecules as compounds of carbon with a variety of functional groups.

b. Universal set of small molecules

c. Macromolecules as the major constituents of cells

d. Configuration and conformation with definitions and suitable examples only.

e. Types of stereo-isomers and importance of stereoisomerism in biology.

f. Types of bonds and their importance: Electrovalence, covalent, ester,

thioester, peptide, glyosidic, phosphodiester.

Water- Structure, properties in brief.

Carbohydrates: Definition, classification, biological role, monosaccharides,

oligosaccharides (maltose, cellobiose, sucrose, lactose) and polysaccharide (starch,

glycogen, peptidoglycan, cellulose)

Lipids: Fatty acids as basic component of lipids and their classification

(Lehninger), nomenclature, storage lipids and structural lipids, types of lipids with

15

Page 11: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

3.5

3.6

general structure of each and mention examples.

Amino acids& proteins: General structure and features of amino acids (emphasis on

amphoteric nature) Classification by R-group, uncommon amino acids and their

functions peptides and proteins- Definition and general features and examples with

biological role. Primary, secondary, tertiary, quaternary structures of proteins- brief

outline.

Nucleic acids: Nitrogenous bases- purines, pyrimidines pentoses - ribose,

deoxyribose, nomenclature of nucleosides and nucleotides, N-β-glycosidic bond,

polynucleotide chain to show bonding between nucleotides (phosphodiester bonds).

Basic structure of RNA and DNA.

COURSE CODE: BUSMB102 (2018-19) (Theory) Credits- 02

Title:- Basic Techniques in Microbiology

Sr.

No.

Modules/Units Lectures

(45)

1.

1.1

1.2

1.3

Microscopy & Staining

Microscopy - History of microscopy

Optical spectrum - Lenses and mirrors: simple and compound light microscope, dark

field Microscopy, phase contrast

Staining procedures –

a. Dyes and stains: types, physicochemical basis fixatives, mordants,

decolorizers

b. Simple and differential staining

c. Special staining (cell wall, capsule, lipid granules spores, metachromatic

granules & flagella)

15

2.

2.1

2.2

Control of Microorganisms

Definition of frequently used terms & rate of microbial death, factors affecting the

effectiveness of antimicrobial agents & properties of an ideal disinfectant

Evaluation of disinfectant –Tube dilution & agar plate techniques, phenol

15

Page 12: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

2.3

2.4

2.5

coefficient, tissue toxicity index

Physical methods of microbial control

a. Dry & moist heat – mechanisms, instruments used and their operations

b. Electromagnetic radiations – ionizing radiations, mechanisms –advantages &

disadvantages

c. Bacteria proof filters

d. Low temperature

e. Osmotic pressure

f. Desiccation

Chemical methods of microbial control – mechanism & advantages &

disadvantages (if any) applications.

a. Phenolics

b. Alcohols

c. Heavy metals and their compounds

d. Halogens

e. Quaternary ammonium compounds

f. Dyes

g. Surface active agents/Detergents

h. Aldehydes,

i. Peroxisomes

j. Sterilizing gases

Chemotherapeutic agents - List types of agents active against various groups &

mention the site of action. (Detailed mode of action not to be done).

3

3.1

3.2

3.3

3.4

3.5

3.6

Microbial Nutrition, Cultivation, Isolation & Preservation

Nutritional requirements – carbon, oxygen, hydrogen, nitrogen, phosphorus, sulfur

and growth factors.

Nutritional types of microorganisms

Types of culture media with examples

Isolation of microorganisms and pure culture techniques

Preservation of microorganisms

Culture collection centers

15

Page 13: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

SECTION - I

COURSE CODE: BUSMBP101 (2018-19) (Practical) Credits- 01

Title:- Fundamentals of Microbiology

Sr.

No.

Modules/Units Lectures

(45)

1.

1. Assignment: Contribution of Scientists in the field of microbiology

2. Special staining: Cell wall, capsule, endospore, flagella, lipid, metachromatic

granules.

15

2. 1. Handling corrosive chemical using rubber teat method for pipetting.

2. Prevention of mouth pipetting and use of auto-pipettes.

3. Discard of highly infectious pathogenic samples like T.B, sputum etc.

4. Explain safety inoculation hood for infection inoculations and laminar air flow.

5. On accidental spillage of/ breakage of culture containers-precautions to be taken.

6. Demonstration of microbes in air, cough, on table surface, finger tips.

7. Permanent slides of eukaryotes & its organelles:

8. Assignment: eukaryotic organelles

15

3. 1. Qualitative detection:

2. Carbohydrates- Benedicts, Molisch’s test.

3. Proteins, amino acids- Biuret, Ninhydrin.

4. Nucleic acid detection by DPA and Orcinol

15

SECTION - II

COURSE CODE: BUSMBP102 (2018-19) (Practical ) Credits- 01

Title:- Basic Techniques in Microbiology

Sr.

No.

Modules/Units Lectures

(45)

1. 1. Parts of a microscope

2. Micrometry

3. Dark field and phase contrast: Demonstration

4. Monochrome and differential staining procedures, Gram Staining & negative

staining

15

2. 1. Introduction to laboratory equipment, disinfection &discarding techniques in

laboratory

15

Page 14: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

2. Methods of preparation of glassware for sterilization(pipettes, petri plates, plastic

wares, flasks, micropipettes, microtiter plates) & control of microorganisms using

moist heat & dry heat sterilization (saterilization of dry powders, rubber gloves,

bandages, screw capped tubes, sterilizable plasticwares)

3. Effect of UV light, desiccation, surface tension, osmotic pressure, heavy

metals (oligodynamic action)

4. Effect of dyes, phenolic compounds and chemotherapeutic agents (disc

inhibition method)

5. Evaluation of disinfectant by Coupon Method

3. 1. Preparation of Culture Media:

a. Liquid medium (nutrient broth)

b. Solid media (nutrient agar, Sabourauds agar)

c. Preparation of slant, butts & plates

2. Inoculation techniques and Study of Growth:

a. Inoculation of liquid Medium

b. Inoculation of solid media (slants, butts and plates)

c. Study of colony characteristics of pigment & non- pigment producing

bacteria.

d. Study of motility (Hanging Drop Preparation)

3. Uses of Differential & Selective Media: (MacConkey & Salt Mannitol Agar)

4. Determination of optimum growth conditions:

a. Temperature, pH

5. Methods of preservation of culture

15

F.Y. B.Sc Semester- II

Microbiology

COURSE CODE: BUSMB201 (2018-19) (Theory) Credits- 02

Title:- Basics of Microbiology

Sr.

No.

Modules/Units Lectures

(45)

1 Study of Different Groups of Microbes-I: 1.1 Viruses a) Historical highlights, general properties of viruses, prions, viroids

b) Structure of viruses-capsids, envelopes, genomes,

c) Cultivation of viruses- overview

d) Bacteriophages: lytic cycle, lysogeny, structure and life cycle of T4 phage.

1.2 Ricketssia, Coxiella, Chlamydia, Mycoplasma:

general features, medical significance

Page 15: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

1.3 Actinomycetes: General features of Nocardia and Streptomyces Importance:

ecological, commercial andmedical

1.4 Archaea: Introduction- Major Archaeal physiological groups, Archaeal cell wall,

lipids and membranes, ecological importance

2. Study of Different Groups of Microbe II: Classification, morphological characteristics, cultivation, reproduction and significance 2.1 Protozoa: Major categories of Protozoa based on motility, reproduction. medically important Protozoa, life cycle of Entamoeba 2.2 Algae – Characteristics of algae: morphology, pigments, reproduction, cultivation of algae, major groups of algae – an overview. biological, medical importance of algae differences between algae and cyanobacteria 2.3 Fungi and Yeast-Characteristics: structure, reproduction, cultivation of fungi and yeasts, major fungal divisions- overview of life cycle of yeast, biological and economical importance 2.4 Slime molds and Myxomycetes

3 3.1 Microbial Growth

a. Definition of growth, mathematical expression, growth curve

b. Measurement of growth

c. Direct microscopic Count – Breed’s count, Petroff-Haussercounting chamber –

Hemocytometer

d. Viable count – spread plate and pour plate techniques

e. Measurement of cell constituents

f. Turbidity measurements – nephelometer and spectrophotometer technique

g. Synchronous growth, continuous growth (chemostat and turbidostat)

h. Influence of environmental factors on growth

i. Microbial growth in natural environment

j. Counting viable non-culturable organisms- quorum sensing techniques

Page 16: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

COURSE CODE: BUSMB202 (2018-19) (Theory) Credits- 02

Title:- EXPLORING MICROBIOLOGY

Sr.

No.

Modules/Units Lectures

(45)

1 Microbial Interactions: 15

1.1 Types of Microbial Interactions: Mutualism, cooperation, commensalisms, predation parasitism, commensalism, competition

1.2 Human Microbe Interactions

a) Normal flora of the human body: skin, nose & nasopharynx, oropharynx,

respiratory tract, eye, external ear, mouth, stomach, small intestine, large intestine,

genitourinary tract.

b) Relationship between microbiota& the host,

c) Gnotobiotic animals

1.3 Microbial associations with vascular plants a) Phyllosphere

b) Rhizosphere & Rhizoplane c) Mycorrhizae

d) Nitrogen fixation: Rhizobia, Actinorhiza, stem nod

ulating Rhizobia

e) Fungal & Bacterial endophytes f) Agrobacterium & other plant pathogens

2. Microbes & Human Health: 2.1 Difference between infection & disease. Important terminology: Primary infection, secondary infection, contagious infection,

occupational disorder, clinical infection, subclinical infection, zoonosis, genetic

disorder, vector borne infection.

2.2 Factors affecting infection: Microbial factors: adherence, invasion, role of virulence factors in invasion,

colonization & its effects.

Host factors: natural resistance, species resistance, racial resistance.

2.3 Individual resistance:

Factors influencing individual resistance: age, nutrition, personal hygiene, stress,

hormones, addiction to drugs/ alcohol. Interaction between Microbes & host is

dynamic.

2.4 Host defense against infection: Overview i) First line of defense: for skin, respiratory tract, gastrointestinal tract, genitourinary

tract, eyes.

ii) Second line of defense: biological barriers: phagocytosis, inflammation

iii) Third line of defense: Brief introduction to antibody mediated & cell mediated

15

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immunity.

3. Advance Techniques In Microbiology &Instrumentation: 3.1 Electron microscope: TEM, SEM 3.2 Contrast enhancement for electron microscope 3.3 Florescent microscope, confocal microscope 3.4 pH meter, pH meter validation and calibration 3.5 Colorimeter 3.6 Validation and calibration of autoclave & hot air oven 3.7 Concepts: Laminar air flow systems, biosafety cabinets, walk-in incubators, industrial autoclaves, cold Room

15

COURSE CODE: BUSMBP2 (2018-19) (Practical) Credits- 01

SECTION - I

Title - BASICS OF MICROBIOLOGY.

Sr.

No.

Modules/Units Lectures

(45)

1. 1. Spot assay and plaque assay of bacteriophages (Demonstration)

2. Slide Culture technique (Actinomycetes & Fungal Culture)

3. Isolation of yeast, cultivation of other fungi, cultivation on Sabourauds agar

15

2. 1. Static & shaker cultures

2. Fungal wet mounts & study of morphological characteristics: Mucor, Rhizopus,

Aspergillus, Penicillium,

3. Permanent slides of Algae, Protozoa

15

3. 1. Growth curve (Demonstration) only in complex media.

2.Breed’s Count

3.Haemocytometer

4.Viable count: Spread plate and pour plate

5.Brown’s opacity

6.Effect of pH and temperature on growth

7.Measurement of cell dimensions-Micrometry

15

Page 18: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

COURSE CODE: BUSMBP2 (2018-19) (Practical) Credits- 01

SECTION – II

Sr.

No.

Modules/Units Lectures

(45)

1. 1. Normal flora of the skin & saliva

2. Wet mount of lichen

3. Bacteroide staining & isolation of Rhizobium

4. Azotobacter isolation & staining

15

2. 6. Study of virulence factors – Enzyme coagulase

7. Study of virulence factors – Enzyme hemolysin

8. Study of virulence factors – Enzyme lecithinase

15

3. 9. Use of standard buffers for calibration and determination of pH of a given solution

10. Determination of & verification of Beer Lambert’s law

11. Determination & efficiency of autoclave, hot air oven, LAF

12. Writing of SOP’s for instruments

13. Visit to a Microbiology laboratory in a research institute

15

REFERENCES: BUSMB 102& BUSMB 202

1. Microbiology TMH 5th Edition by Michael J. Pelczar Jr., E.C.S. Chan, Noel R. Krieg

2. A.J. Salle, Fundamental Principles of Bacteriology, McGraw Hill Book Company Inc.1984

Cruikshank, Medical Microbiology, Vol -II

3. Prescott, Hurley, Klein-Microbiology, 5th & 6th edition, International edition 2002 & 2006,

McGraw Hill.

4. Michael T. Madigan & J.M. Martin, Brock, Biology of Microorganisms 11th Ed. International

edition ,2006, Pearson Prentice Hall

Page 19: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

Modality of Assessment Assessment pattern for theory

Scheme of Examination

The learner’s Performance shall be assessed by conducting the Internal assessment marks (40 % includes

Internal Class Test 15 Marks, Project or Assignment 20 Marks, and Overall performance (Attendance and

Sincerity of the students) 05 marks and Semester End Examinations with 60 % marks.

Internal Unit Test (15 Marks)

1. Internal Unit test shall be of 30 minutes of duration carries 15 Marks

2. It consists of three questions or One question with three sub-section as 1, 2 and 3 or a, b and c. Each

question or Sub- section will be of 5 marks.

3. First question or first Sub-section having five objective type question with four multiple options.

4. Second question or sub-section will have define the terms or answer in one to sentences or

cross the match of five marks

5. Third question or sub-section will consist of three short notes out of which any one short note to solve

for five marks.

Semester End Theory Assessment – 60 % 60 marks

1. Duration - These examinations shall be of 2hours duration.

2. Theory question paper pattern: -

i. There shall be four questions each of 15 marks (with internal options)

ii. Each 15 Marks Question will be based on each unit, question one Unit-I, question Unit-II,

question three on Unit-III, question four will have questions from all three units (Unit-I,

Unit-II and Unit-III) of the respective paper syllabus.

iii. Each of the main questions on Unit-I, Unit-II & Unit-III consists of six questions and you

will have to solve any three questions out of six. Each question carries five marks.

iv. Question number four consist of questions from all the three units (Unit-I, Unit-II and Unit-

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III) from respective paper as three subsections 1, 2 and 3 or a, b and c. Each subsection will

consist of five marks of question on give examples, define the terms and Give Significance

or Contributions of Scientist.

v. All questions shall be compulsory with internal choice within the questions.

vi. The allocation of marks will depend on the weightage of the topic.

Passing Standard:

The learners to pass a course shall have to obtain a minimum of 40% marks in aggregate for each

course and 40% marks in Semester End Examination (i.e. 40 out of 100) separately, to pass the

course and minimum of Grade E in each project, wherever applicable, to pass a particular

semester.

Practical Examination Pattern:

External (Semester end practical examination):-

Sr. No. Particulars/ Paper Marks

1. Laboratory work 40

2. Journal 05

3. Viva 05

Semester I:

The students are required to present a duly certified journal for appearing at the practical examination,

failing which they will not be allowed to appear for the examination.

In case of loss of Journal and / or Report, a Lost Certificate should be obtained from the Head of

the Department / Coordinator of the department; failing which the student will not be allowed to

appear for the practical examination.

Semester II

The students are required to present a duly certified journal for appearing at the practical examination,

failing which they will not be allowed to appear for the examination.

Page 21: B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE ... · B. K. BIRLA COLLEGE OF ARTS, SCIENCE AND COMMERCE (AUTONOMOUS) KALYAN (W) Affiliated to University of Mumbai Choice Based

In case of loss of Journal and/ or Report, a Lost Certificate should be obtained from the Head of

the Department/ Coordinator of the department; failing which the student will not be allowed to

appear for the practical examination. Overall Examination and Marks Distribution Pattern.

Overall Examination and Marks Distribution Pattern

Semester I

Course BUSMB-101 BUSMB-102 Grand Total

Internal Exam Marks 40

(Internal Class Test 15 Marks, Project or Assignment 20 Marks &

Overall Performance 5 Marks)

40 Marks 40 Marks 80

Theory Exam 60 Marks 60 Marks 120

Practicals 50 Marks 50 Marks 100

Semester II

Course BUSMB-201 BUSMB-202 Grand

Total

Internal Exam Marks 40

(Internal Class Test 15 Marks, Project or Assignment 20 Marks &

Overall Performance 5 Marks)

40 Marks 40 Marks 80

Theory Exam 60 Marks 60 Marks 120

Practicals 50 Marks 50 Marks 100