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From: Methods in Molecular Biology, vol. 338: Baculovirus and Insect Cell Expression Protocols, 2/eEdited by: D. W. Murhammer © Humana Press Inc., Totowa, NJ
6
Available Lepidopteran Insect Cell Lines
Dwight E. Lynn
SummaryThis chapter lists the known cell lines from Lepidoptera, largely based on previous
compilations of insect cell lines published by W. Fred Hink. The official designation isgiven for each cell line as well as the species, tissue source, and, when known, the sus-ceptibilities to baculoviruses.
Key Words: Lepidoptera; continuous cell lines; insect cells; virus susceptibility.
1. IntroductionEarly in the history of insect cell culturing, researchers in the field began
meeting at 3- to 4-yr intervals at International Conferences on InvertebrateTissue Culture. The first of these was held in Montpellier, France in 1962,which, perhaps not coincidentally, was the year that the first continuous insectcell lines were described in the literature (1). In the 1970s and 1980s, W.Frederick Hink prepared compilations of insect cell lines (2–6) that wereincluded in the proceedings from several of the subsequent InternationalConferences. His lists form the backbone of the listing included in this chapter(Table 1). Unfortunately, the last of his compilations was published 15 yr ago,so a literature search has been performed for publications since that time. Asseen in Fig. 1, the availability of lepidopteran cell lines has steadily increasedat about 50 new lines per decade. In addition to details on the insect species,designation of the resulting cell lines, and tissue source used, details on thesusceptibility to baculoviruses have also been provided where they are known.Finally, the baculoviruses that have been grown in insect cell culture are sum-marized in Table 2. Note that a given baculovirus is named based on the insectspecies from which it was first isolated.
Tabl
e 1
Lepi
dopt
eran
Cel
l Lin
es E
Sp
eci
es
De
sig
na
tion
Tis
sue
so
urc
eB
acu
lovi
rus
infe
ctiv
itya
Re
f.b
Ado
xoph
yes
oran
aA
o/I
Ad
ult
ova
rie
sA
cMN
PV
,N
P-1
Ad
orM
NP
V,
Mb
MN
PV
,S
fMN
PV
Ado
xoph
yes
oran
aF
TR
S-A
oL
1N
eo
na
te la
rva
eA
do
rMN
PV
, P
lxyM
NP
V8
fasc
iata
FT
RS
-Ao
L2
Ne
on
ate
larv
ae
Ad
orM
NP
V,
Plx
yMN
PV
8A
doxo
phye
s sp
.F
TR
S-A
fLN
eo
na
te la
rva
eA
do
rMN
PV
, P
lxyM
NP
V8
Agr
otis
ipsi
lon
BC
IRL
/AM
CY
-AiO
V-C
LG
Ad
ult
ova
rie
s a
nd
fa
t b
od
yP
lxyM
NP
V9
BC
IRL
/AM
CY
-AiT
S-C
LG
Ad
ult
test
es
an
d f
at
bo
dy
9A
mye
lois
tran
site
lla
HC
RL
-AT
O1
0P
up
al o
vari
es
AcM
NP
V10
HC
RL
-AT
O2
0P
up
al o
vari
es
AcM
NP
V10
Ana
grap
ha f
alci
fera
BC
IRL
/AM
CY
-AfO
V-C
LG
Ad
ult
ova
rie
s a
nd
fa
t b
od
yA
cMN
PV
9B
CIR
L/A
MC
Y-A
fTS
-CL
GA
du
lt te
ste
s a
nd
fa
t b
od
y9
Ant
hera
ea e
ucal
ypti
RM
L-2
su
blin
e o
f G
race
’sP
up
al o
vari
es
Bm
NP
V1
A. e
ucal
ypti
ce
l lsP
up
al o
vari
es
1A
nthe
raea
per
nyi
Pu
pa
l ova
rie
sN
P-2
NIS
ES
-An
Pe
-42
6E
mb
ryo
s11
Ant
hera
ea y
amam
aiN
ISE
S-A
nya
-06
11
Pu
pa
l ova
rie
sA
nya
NP
V12
Ant
icar
sia
gem
mat
alis
BC
IRL
/AM
CY
-Ag
E-C
LG
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-Ag
OV
-CL
G1
Ad
ult
ova
rie
s a
nd
fa
t b
od
yA
gM
NP
V9
BC
IRL
/AM
CY
-Ag
OV
-CL
G2
Ad
ult
ova
rie
s a
nd
fa
t b
od
yA
cMN
PV
, A
gM
NP
V9
BC
IRL
/AM
CY
-Ag
OV
-CL
G3
Ad
ult
ova
rie
s a
nd
fa
t b
od
yA
cMN
PV
, A
gM
NP
V9
UF
L-A
G-2
86
Em
bry
os
AcM
NP
V,
Ag
MN
PV
,13
An
faM
NP
V,
Gm
MN
PV
,H
ea
rMN
PV
, P
lxyM
NP
V,
Ro
MN
PV
118
Arc
hipp
us b
revi
plic
anus
FT
RS
-Ab
L8
1N
eo
na
te la
rva
e8
Bom
byx
man
dari
naS
ES
-Bm
a-O
1A
Ma
ture
em
bry
os
14S
ES
-Bm
a-O
1R
Ma
ture
em
bry
os
14B
omby
x m
ori
Bm
-NB
mN
PV
NP
-3L
arv
al m
idg
uts
15B
m5
La
rva
l ova
rie
s16
Bm
-21
E-H
NU
5E
mb
ryo
sA
cMN
PV
, A
rNP
V,
He
arM
NP
V,
Plx
yGV
17N
IV-B
M-1
29
6L
arv
al o
vari
es
AcM
NP
V,
Bm
NP
V18
NIV
-BM
-19
7P
up
al o
vari
es
AcM
NP
V,
Bm
NP
V18
SE
S-B
m-1
30
AM
atu
re e
mb
ryo
s14
SE
S-B
m-1
30
RM
atu
re e
mb
ryo
s14
SE
S-B
m-e
21
AM
atu
re e
mb
ryo
s14
SE
S-B
m-e
21
BM
atu
re e
mb
ryo
s14
SE
S-B
m-e
21
RM
atu
re e
mb
ryo
s14
SE
S-B
oM
o-1
5A
Em
bry
os
Bm
NP
V19
SE
S-B
oM
o-C
12
9E
mb
ryo
sB
mN
PV
20S
ES
-Bo
Mo
-JI2
5E
mb
ryo
s20
SP
C-B
m3
6P
up
al o
vari
es
NP
-4S
PC
-Bm
40
Pu
pa
l ova
rie
sN
P-4
Buz
ura
supp
ress
aria
WIV
-BS
-48
1L
arv
al h
em
ocy
tes
NP
-5W
IV-B
S-4
84
Ima
gin
al o
vari
es
Bu
suN
PV
NP
-5C
hilo
sup
pres
sali
sL
arv
al h
em
ocy
tes
21C
hori
ston
eura
FP
MI-
CF
-1M
idg
uts
22 fu
mif
eran
aF
PM
I-C
F-2
Mid
gu
ts22
FP
MI-
CF
-3M
idg
uts
22F
PM
I-C
F-2
03
Mid
gu
ts22
119
(con
tinu
ed)
Tabl
e 1
(Con
tinu
ed)
Sp
eci
es
De
sig
na
tion
Tis
sue
so
urc
eB
acu
lovi
rus
infe
ctiv
itya
Re
f.b
FP
MI-
CF
-50
Pu
pa
l ova
rie
sA
cMN
PV
, C
fMN
PV
NP
-6F
PM
I-C
F-6
0P
up
al o
vari
es
AcM
NP
V,
CfM
NP
VN
P-6
FP
MI-
CF
-70
Pu
pa
l ova
rie
sA
cMN
PV
, C
fMN
PV
NP
-6IP
RI-
CF
-1N
eo
na
te la
rva
eC
fMN
PV
NP
-6IP
RI-
CF
-10
Ne
on
ate
larv
ae
CfM
NP
VN
P-6
IPR
I-C
F-1
2N
eo
na
te la
rva
eC
fMN
PV
NP
-6IP
RI-
Cf1
24
La
rva
eC
fMN
PV
23IP
RI-
CF
-16
Ne
on
ate
larv
ae
CfM
NP
VN
P-6
IPR
I-C
F-1
6T
Ne
on
ate
larv
ae
NP
-6IP
RI-
CF
-5N
eo
na
te la
rva
eC
fMN
PV
NP
-6IP
RI-
CF
-6N
eo
na
te la
rva
eC
fMN
PV
NP
-6IP
RI-
CF
-8N
eo
na
te la
rva
eC
fMN
PV
NP
-6C
hori
ston
eura
Em
bry
os,
ne
on
ate
larv
ae
CfM
NP
VN
P-6
occi
dent
alis
a
nd
ova
rie
sC
ydia
pom
onel
laC
P-1
26
8E
mb
ryo
s24
CP
-16
9E
mb
ryo
s24
Cp
DW
1E
mb
ryo
s25
Cp
DW
2E
mb
ryo
s25
Cp
DW
3E
mb
ryo
s25
Cp
DW
4E
mb
ryo
s25
Cp
DW
5E
mb
ryo
s25
Cp
DW
6E
mb
ryo
s25
Cp
DW
9E
mb
ryo
s25
Cp
DW
10
Em
bry
os
25C
pD
W1
1E
mb
ryo
s25
Cp
DW
12
Em
bry
os
25
120
Cp
DW
13
Em
bry
os
25C
pD
W1
4E
mb
ryo
sC
pG
V25
Cp
DW
15
Em
bry
os
Cp
GV
252
00
“p
rim
ary
” ce
ll lin
es
Em
bry
os
an
d la
rva
lC
hm
uN
PV
, C
pG
V26
h
em
ocy
tes
IZD
-Cp
4/1
327
IZD
-CP
15
08
La
rva
l he
mo
cyte
sN
P-7
IZD
-CP
22
02
La
rva
l he
mo
cyte
sN
P-7
IZD
-CP
25
07
La
rva
l he
mo
cyte
sN
P-7
IZD
-CP
05
08
La
rva
l he
mo
cyte
sN
P-7
Ect
ropu
s ob
liqu
aS
IE-E
O-8
01
Pu
pa
l ova
rie
s28
SIE
-EO
-80
3P
up
al o
vari
es
28E
phes
tia
kueh
niel
laIP
LB
-Ekx
4T
Em
bry
os
AcM
NP
V,
Ag
MN
PV
,29
An
faM
NP
V,
Gm
MN
PV
,H
ea
rMN
PV
, P
lxyM
NP
V,
Ro
MN
PV
IPL
B-E
kx4
VE
mb
ryo
sA
cMN
PV
, A
gM
NP
V,
29A
nfa
MN
PV
, G
mM
NP
V,
He
arM
NP
V,
Plx
yMN
PV
,R
oM
NP
VE
stig
men
e ac
rea
EA
11
74
A (
=B
TI-
EA
A)
La
rva
l he
mo
cyte
sA
cMN
PV
30E
A1
17
4H
La
rva
l he
mo
cyte
sA
cMN
PV
30E
uxoa
sca
nden
sIA
FE
s-1
Ova
rie
sA
cMN
PV
, B
mN
PV
,31
Gm
MN
PV
, D
iwa
NP
VG
alle
ria
mel
lone
lla
Ova
rie
s32
Gno
rim
osch
ema
G0
1-8
74
Em
bry
os
33op
ercu
lell
aP
TM
Em
bry
os
NP
-8
(con
tinu
ed)
121
Tabl
e 1
(Con
tinu
ed)
Sp
eci
es
De
sig
na
tion
Tis
sue
so
urc
eB
acu
lovi
rus
infe
ctiv
itya
Re
f.b
Hel
icov
erpa
arm
iger
aB
CIR
L-H
A-A
M1
Pu
pa
l ova
rie
sA
gM
NP
V34
CS
IRO
-BC
IRL
-HA
1O
vari
es
AcM
NP
V,
HzS
NP
V35
CS
IRO
-BC
IRL
-HA
2O
vari
es
HzS
NP
V35
CS
IRO
-BC
IRL
-HA
3O
vari
es
HzS
NP
V35
NIV
-HA
-19
7E
mb
ryo
AcM
NP
V,
He
arS
NP
V,
36S
pltM
NP
VH
elic
over
pa p
unct
iger
aC
SIR
O-B
CIR
L-H
P1
Em
bry
os
AcM
NP
V,
HzS
NP
V35
CS
IRO
-BC
IRL
-HP
2E
mb
ryo
sA
cMN
PV
, H
zSN
PV
35C
SIR
O-B
CIR
L-H
P3
Em
bry
os
AcM
NP
V,
HzS
NP
V35
CS
IRO
-BC
IRL
-HP
4O
vari
es
AcM
NP
V,
HzS
NP
V35
CS
IRO
-BC
IRL
-HP
5O
vari
es
AcM
NP
V,
HzS
NP
V35
Hel
icov
erpa
zea
BC
IRL
/AM
CY
-HzE
-CL
G1
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-HzE
-CL
G2
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-HzE
-CL
G3
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-HzE
-CL
G5
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-HzE
-CL
G6
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-HzE
-CL
G7
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-HzE
-CL
G8
Em
bry
os
9B
CIR
L/A
MC
Y-H
zE-C
LG
9E
mb
ryo
s9
BC
IRL
-HZ
-AM
1P
up
al o
vari
es
HzS
NP
V,
He
arS
NP
V34
BC
IRL
-HZ
-AM
2P
up
al o
vari
es
HzS
NP
V34
BC
IRL
-HZ
-AM
3P
up
al o
vari
es
HzS
NP
V34
IMC
-HZ
-1A
du
lt o
vari
es
HzS
NP
V37
IPL
B-H
Z-1
07
4P
up
al o
vari
es
HzM
NP
V38
IPL
B-H
Z-1
07
5P
up
al o
vari
es
an
d f
at
bo
dy
HzM
NP
V38
IPL
B-H
Z-1
07
9F
at
bo
dy
HzM
NP
V38
122
IPL
B-H
Z-1
10
Pu
pa
l ova
rie
sH
zMN
PV
38IP
LB
-HZ
-12
4Q
Pu
pa
l ova
rie
sH
zMN
PV
38H
elio
this
vir
esce
nsB
CIR
L/A
MC
Y-H
vE-C
LG
1E
mb
ryo
sA
cMN
PV
, A
gM
NP
V,
9P
lxyM
NP
VB
CIR
L/A
MC
Y-H
vE-C
LG
2E
mb
ryo
sA
cMN
PV
, A
gM
NP
V9
Plx
yMN
PV
BC
IRL
/AM
CY
-HvE
-CL
G3
Em
bry
os
AcM
NP
V,
Ag
MN
PV
9B
CIR
L/A
MC
Y-H
vOV
-CL
GA
du
lt o
vari
es
AcM
NP
V,
Ag
MN
PV
,9
Plx
yMN
PV
BC
IRL
/AM
CY
-Hv-
TS
-GE
SL
arv
al t
est
es
AcM
NP
V9
BC
IRL
-HV
-AM
1P
up
al o
vari
es
AcM
NP
V,
Ag
MN
PV
,39
HzS
NP
VB
CIR
L-H
V-A
M2
Pu
pa
l ova
rie
sA
cMN
PV
, H
zSN
PV
39IP
LB
-HvE
1a
Em
bry
os
AcM
NP
V,
An
faM
NP
V,
40A
gM
NP
V,
HzS
NP
V,
Plx
yMN
PV
, R
oM
NP
VIP
LB
-HvE
1-l
tE
mb
ryo
sA
cMN
PV
, A
nfa
MN
PV
,40
Ag
MN
PV
, H
zSN
PV
,O
pM
NP
V,
Ro
MN
PV
IPL
B-H
vE1
sE
mb
ryo
sA
cMN
PV
, A
nfa
MN
PV
,40
Ag
MN
PV
, H
zSN
PV
,P
lxyM
NP
V,
Ro
MN
PV
IPL
B-H
vE6
aE
mb
ryo
sA
cMN
PV
, A
nfa
MN
PV
,40
Ag
MN
PV
, H
zSN
PV
,P
lxyM
NP
V,
Ro
MN
PV
IPL
B-H
vE6
a-l
tE
mb
ryo
sA
cMN
PV
, A
nfa
MN
PV
,40
Ag
MN
PV
, H
zSN
PV
,O
pM
NP
V,
Plx
yMN
PV
,R
oM
NP
V
(con
tinu
ed)
123
Tabl
e 1
(Con
tinu
ed)
Sp
eci
es
De
sig
na
tion
Tis
sue
so
urc
eB
acu
lovi
rus
infe
ctiv
itya
Re
f.b
IPL
B-H
vE6
sE
mb
ryo
sA
cMN
PV
, A
nfa
MN
PV
,40
Ag
MN
PV
, H
zSN
PV
,P
lxyM
NP
V,
Ro
MN
PV
IPL
B-H
vE6
s-lt
Em
bry
os
AcM
NP
V,
An
faM
NP
V,
40A
gM
NP
V,
HzS
NP
V,
Ro
MN
PV
IPL
B-H
vT1
La
rva
l te
stic
ula
r sh
ea
thA
cMN
PV
, H
zSN
PV
41H
omon
a m
agna
nim
aF
TR
S-H
mL
45
Ne
on
ate
larv
ae
Plx
yMN
PV
8H
oshi
noa
long
icel
lana
FT
RS
-HlL
1N
eo
na
te la
rva
e8
FT
RS
-HlL
2N
eo
na
te la
rva
e8
Lat
oia
viri
diss
ima
La
rva
l he
mo
lym
ph
La
viN
PV
42L
euca
nia
sepa
rata
NIA
S-L
eS
e-1
1L
arv
al f
at
bo
dy
(fe
ma
le)
AcM
NP
V43
Lym
antr
ia d
ispa
rIP
LB
-LD
-64
Pu
pa
l ova
rie
sA
cMN
PV
44IP
LB
-LD
-65
Pu
pa
l ova
rie
sL
dM
NP
V44
IPL
B-L
D-6
6P
up
al o
vari
es
44IP
LB
-LD
-67
Pu
pa
l ova
rie
sL
dM
NP
V44
IPL
B-L
dE
GE
mb
ryo
sA
cMN
PV
, L
dM
NP
V41
IPL
B-L
dE
IE
mb
ryo
sA
cMN
PV
, L
dM
NP
V41
IPL
B-L
dE
ItE
mb
ryo
sA
cMN
PV
, L
dM
NP
V41
IPL
B-L
dE
pE
mb
ryo
sA
cMN
PV
, L
dM
NP
V41
IPL
B-L
dF
BL
arv
al f
at
bo
die
sL
dM
NP
V41
IZD
-LD
13
07
La
rva
l te
ste
sN
P-9
IZD
-LD
14
07
La
rva
l te
ste
sA
cMN
PV
NP
-9S
CL
d 1
35
Ova
rie
sB
mN
PV
, G
mM
NP
VN
P-9
Mal
acos
oma
diss
tria
IPR
I 1
08
La
rva
l he
mo
cyte
sA
cMN
PV
, C
fMN
PV
,45
La
fiso
NP
V
124
UM
N-M
DH
-1.
He
mo
cyte
s o
f fif
th-i
nst
ar
Ma
diN
PV
NP
-10
la
rva
eM
ames
tra
bras
sica
eH
PB
-MB
Ad
ult
ova
rie
sA
cMN
PV
, T
nS
NP
VN
P-1
1IZ
D-M
B0
50
3L
arv
al h
em
ocy
tes
AcM
NP
V,
Mb
MN
PV
46IZ
D-M
B0
50
4L
arv
al h
em
ocy
tes
AcM
NP
V46
IZD
-MB
12
03
La
rva
l ova
ry a
nd
do
rsa
l ve
sse
lA
cMN
PV
46IZ
D-M
B2
00
6L
arv
al h
em
ocy
tes
46IZ
D-M
B2
00
7L
arv
al h
em
ocy
tes
46IZ
D-M
B2
50
6L
arv
al h
em
ocy
tes
AcM
NP
V,
Mb
MN
PV
46M
B-H
26
0H
em
ocy
tes
Mb
MN
PV
47M
B-H
26
0H
em
ocy
tes
Mb
MN
PV
47M
bL
-3N
eo
na
te la
rva
eM
bM
NP
V48
Mb
L-3
Ne
on
ate
larv
ae
Mb
MN
PV
48N
IAS
-Ma
Br-
85
La
rva
l fa
t b
od
y (m
ale
)A
cMN
PV
49N
IAS
-Ma
Br-
92
La
rva
l he
mo
cyte
sA
cMN
PV
50N
IAS
-Ma
Br-
93
La
rva
l he
mo
cyte
sA
cMN
PV
50N
IAS
-MB
-19
Pu
pa
l ova
rie
s51
NIA
S-M
B-2
5P
up
al o
vari
es
51N
IAS
-MB
-32
Pu
pa
l ova
rie
s51
SE
S-M
aB
r-1
La
rva
l fa
t b
od
yA
cMN
PV
52S
ES
-Ma
Br-
2L
arv
al f
at
bo
dy
AcM
NP
V52
SE
S-M
aB
r-3
La
rva
l fa
t b
od
yA
cMN
PV
52S
ES
-Ma
Br-
4L
arv
al f
at
bo
dy
AcM
NP
V52
SE
S-M
aB
r-5
La
rva
l fa
t b
od
yA
cMN
PV
52M
andu
ca s
exta
FP
MI-
MS
-12
Ne
on
ate
larv
ae
AcM
NP
VN
P-6
FP
MI-
MS
-4N
eo
na
te la
rva
eA
cMN
PV
NP
-6F
PM
I-M
S-5
Ne
on
ate
larv
ae
AcM
NP
VN
P-6
FP
MI-
MS
-7N
eo
na
te la
rva
eA
cMN
PV
NP
-6M
RR
L-C
H-1
Em
bry
os
53M
RR
L-C
H-2
Em
bry
os
53
(con
tinu
ed)
125
Tabl
e 1
(Con
tinu
ed)
Sp
eci
es
De
sig
na
tion
Tis
sue
so
urc
eB
acu
lovi
rus
infe
ctiv
itya
Re
f.b
Myt
him
na c
onve
cta
BP
MN
U-M
yCo
-1F
at
bo
dy
54O
rgyi
a le
ucos
tigm
aIP
LB
-OlE
50
5A
Em
bry
os
AcM
NP
V,
An
faM
NP
V,
55O
pM
NP
V,
Orl
eN
PV
,R
oM
NP
VIP
LB
-OlE
50
5s
Em
bry
os
Op
MN
PV
, O
rle
NP
V55
IPL
B-O
lE7
Em
bry
os
Op
MN
PV
, O
rle
NP
V55
IPR
I-O
L-1
2N
eo
na
te la
rva
eO
pM
NP
V,
Op
SN
PV
56IP
RI-
OL
-13
Ne
on
ate
larv
ae
Op
MN
PV
, O
pS
NP
V56
IPR
I-O
L-4
Ne
on
ate
larv
ae
Op
MN
PV
, O
pS
NP
V56
IPR
I-O
L-9
Ne
on
ate
larv
ae
Op
MN
PV
, O
pS
NP
V56
Ost
rini
a nu
bila
lis
BC
IRL
/AM
CY
-On
FB
-GE
S1
La
rva
l fa
t b
od
y9
BC
IRL
/AM
CY
-On
FB
-GE
S2
La
rva
l fa
t b
od
y9
UM
C-O
nE
Em
bry
os
AcM
NP
V,
Plx
yMN
PV
57P
ande
mis
hep
aran
aF
TR
S-P
hL
Ne
on
ate
larv
ae
Plx
yMN
PV
8P
apil
io x
uthu
sP
x-5
8P
up
al o
vari
es
58P
x-6
4P
up
al o
vari
es
58P
htho
rim
aea
OR
S-P
op
-93
Em
bry
os
AcM
NP
V,
Sp
l iNP
V59
oper
cule
lla
OR
S-P
op
-95
Em
bry
os
Ph
op
GV
, S
pl iG
V59
Pie
ris
rapa
eB
TI-
PR
10
BE
mb
ryo
s60
BT
I-P
R8
A1
Em
bry
os
60B
TI-
PR
8A
2E
mb
ryo
s60
BT
I-P
R9
AE
mb
ryo
s60
NIA
S-P
RC
-81
9A
Ova
rie
s61
NIA
S-P
RC
-81
9B
Ova
rie
s61
NIA
S-P
RC
-81
9C
Ova
rie
s61
126
NY
AE
S-P
R4
AE
mb
ryo
s60
Plo
dia
inte
rpun
ctel
laIA
L-P
ID2
Ima
gin
al w
ing
dis
csA
gM
NP
V62
IPL
B-P
iEE
mb
ryo
sN
P-1
2U
MN
-PIE
-11
81
Em
bry
os
of
a m
ala
thio
n-
re
sist
an
t st
rain
63P
lute
lla
xylo
stel
laB
CIR
L/A
MC
Y-P
xE-C
LG
Em
bry
os
AcM
NP
V,
Plx
yMN
PV
7B
CIR
L/A
MC
Y-P
xLP
-CL
GL
arv
ae
/pu
pa
e (
wh
ole
AcM
NP
V,
Plx
yMN
PV
7
inse
cts)
IPL
B-P
xE1
Em
bry
os
AcM
NP
V,
An
faM
NP
V,
NP
-12
Ro
MN
PV
IPL
B-P
xE2
Em
bry
os
AcM
NP
V,
An
faM
NP
V,
NP
-12
Ro
MN
PV
PX
-11
87
Em
bry
os
63B
CIR
L-P
X2
-HN
U3
AcM
NP
V,
ArG
V,
ArN
PV
,H
ea
rMN
PV
65P
seud
alet
ia u
nipu
ncta
BT
I-P
u-2
Em
bry
os
AcM
NP
V66
BT
I-P
u-A
7E
mb
ryo
sA
cMN
PV
66B
TI-
Pu
-A7
SE
mb
ryo
sA
cMN
PV
66B
TI-
Pu
-B9
Em
bry
os
AcM
NP
V66
BT
I-P
u-M
Em
bry
os
AcM
NP
V66
BT
I-P
u-M
1B
Em
bry
os
AcM
NP
V66
Sam
ia c
ynth
iaS
eve
ral l
ine
sP
up
al h
em
ocy
tes
67Sp
ilar
ctia
NIA
S-S
pS
e-1
La
rva
l fa
t b
od
y (m
ale
)68
seri
atop
unct
ata
Spil
osom
a im
pari
lis
FR
I-S
pIm
-12
29
La
rva
l fa
t b
od
ies
Hyc
uN
PV
, S
pim
NP
V69
Spod
opte
ra e
xigu
aB
CIR
L/A
MC
Y-S
eE
-CL
G1
Em
bry
os
9B
CIR
L/A
MC
Y-S
eE
-CL
G4
Em
bry
os
BC
IRL
/AM
CY
-Se
E-C
LG
5E
mb
ryo
s9
Se
3F
HN
eo
na
te la
rva
eS
eM
NP
V70
(con
tinu
ed)
127
Tabl
e 1
(Con
tinu
ed)
Sp
eci
es
De
sig
na
tion
Tis
sue
so
urc
eB
acu
lovi
rus
infe
ctiv
itya
Re
f.b
Se
4F
HN
eo
na
te la
rva
eS
eM
NP
V70
Se
5F
HN
eo
na
te la
rva
eS
eM
NP
V70
Se
6F
HA
Ne
on
ate
larv
ae
Se
MN
PV
70S
e6
FH
BN
eo
na
te la
rva
eS
eM
NP
V70
Se
He
92
0-1
aH
em
ocy
tes
71U
CR
-SE
-1N
eo
na
te la
rva
eA
cMN
PV
, S
eM
NP
V72
Spod
opte
ra fr
ugip
erda
BC
IRL
/AM
CY
-SfT
S-G
ES
La
rva
l te
ste
sA
cMN
PV
, P
lxyM
NP
V9
IAL
-SF
D1
Ima
gin
al w
ing
dis
csA
cMN
PV
, A
gM
NP
V,
62T
nS
NP
V,
IPL
B-S
f12
54
Pu
pa
l ova
ryA
cMN
PV
, T
nS
NP
V,
SfM
NP
V73
IPL
B-S
f21
, IP
LB
-Sf2
1A
E,
Sf-
9Pu
pa
l ova
ryA
cMN
PV
, P
lxyM
NP
V,
73S
fMN
PV
, S
pliN
PV
,T
ho
rNP
VSp
odop
tera
litt
oral
isH
PB
-SL
La
rva
eA
cMN
PV
, T
nS
NP
VN
P-1
1S
PC
-Sl-
48
Pu
pa
l ova
rie
sA
cMN
PV
74S
PC
-Sl-
52
Pu
pa
l ova
rie
sA
cMN
PV
74U
IV-S
L-3
73
Pu
pa
l ova
rie
sA
cMN
PV
, S
eM
NP
V,
75S
pliN
PV
, T
nS
NP
VU
IV-S
L-5
73
Pu
pa
l ova
rie
sA
cMN
PV
, S
eM
NP
V,
75S
pliN
PV
, T
nS
NP
VU
IV-S
L-6
73
Pu
pa
l ova
rie
sA
cMN
PV
, S
eM
NP
V,
75S
pliN
PV
, T
nS
NP
VSp
odop
tera
lit
ura
IBL
-SL
1A
Pu
pa
l ova
rie
sS
pltN
PV
76N
IV-S
U-8
93
Pu
pa
l ova
rie
s77
NIV
-SU
-99
2L
arv
al o
vari
es
78Sp
odop
tera
orn
itho
gall
iB
CIR
L-5
03
-HN
U1
Ad
ult
ova
rie
s79
BC
IRL
-50
4-H
NU
4A
du
lt o
vari
es
79
128
Tri
chop
lusi
a ni
B
CIR
L/A
MC
Y-T
nE
-CL
G1
Em
bry
os
AcM
NP
V
9B
CIR
L/A
MC
Y-T
nE
-CL
G1
MK
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-Tn
E-C
LG
2E
mb
ryo
sA
cMN
PV
9B
CIR
L/A
MC
Y-T
nE
-CL
G2
MK
Em
bry
os
AcM
NP
V,
Plx
yMN
PV
9B
CIR
L/A
MC
Y-T
nE
-CL
G3
Em
bry
os
AcM
NP
V9
BC
IRL
/AM
CY
-Tn
TS
-GE
S1
La
rva
l te
ste
sA
cMN
PV
, P
lxyM
NP
V9
BC
IRL
/AM
CY
-Tn
TS
-GE
S3
La
rva
l te
ste
sP
lxyM
NP
V9
BT
I-T
N5
B1
-4(H
igh
Fiv
e®)
Em
bry
os
AcM
NP
V,
Plx
yMN
PV
,80
Th
orN
PV
, T
nS
NP
VB
TI-
TN
5C
1E
mb
ryo
s80
BT
I-T
N5
F2
Em
bry
os
80B
TI-
TN
5G
2A
1E
mb
ryo
s80
BT
I-T
N5
G3
Em
bry
os
80B
TI-
TN
5G
33
Em
bry
os
80IA
L-T
ND
1Im
ag
ina
l win
g d
iscs
AcM
NP
V,
An
faM
NP
V,
81A
gM
NP
V,
Plx
yMN
PV
IPL
B-T
N-R
23
-d-o
ld e
mb
ryo
sA
cMN
PV
, A
nfa
MN
PV
,82
Ag
MN
PV
, P
lxyM
NP
VS
eve
ral l
ine
sP
up
al o
vary
an
d f
at
bo
dy
Tn
SN
PV
83T
N-3
68
Ad
ult
ova
rie
sA
cMN
PV
, T
nS
NP
V,
84G
mM
NP
V,
Ag
MN
PV
,P
lxyM
NP
V
a In
mo
st c
ase
s, t
he
vir
us
susc
ep
tibili
ties
we
re r
ep
ort
ed
in
th
e o
rig
ina
l p
ub
lica
tion
of
the
ce
ll lin
es.
In
so
me
ca
ses,
th
ey
are
fr
om
th
e H
ink
com
pila
tion
s (2–
6) o
r th
e G
ran
ad
os
an
d H
ash
imo
to r
evi
ew
(7
) (s
ee N
ote
1).
b NP
, no
t pu
blis
he
d. M
ost
of t
he
se c
ell
line
s w
ere
re
po
rte
d in
the
Hin
k co
mp
ilatio
ns
(2–6
). T
he
re
sea
rch
er(
s) th
at c
om
mu
nic
ate
d th
e c
ell
line
toH
ink
we
re a
s fo
llow
s: N
P-1
: D
. P
ete
rs,
NP
-2:
H.
Le
e,
NP
-4:
J.M
. Q
uio
t, N
P-5
: X
ie T
ian
en
, W
an
g L
um
ing
, a
nd
Liu
So
ng
hu
s, N
P-6
: S
. S
. S
oh
i,N
P-7
: U
. M
ah
r a
nd
H.
G.
Milt
en
bu
rge
r, N
P-8
: T
. D
. C
. G
race
, N
P-9
: H
.G.
Milt
en
bu
rge
r, N
P-1
0:
K.
R.
Tsa
ng
, N
P-1
1:
I. H
ilwig
an
d F
. A
lap
att
,N
P-1
2:
D.
E.
Lyn
n.
NP
-3:
the
B
. mor
i Bm
-N li
ne
is w
ide
ly d
istr
ibu
ted
an
d u
sed
with
Bm
MN
PV
bu
t I
ha
ve b
ee
n u
na
ble
to
dis
cern
th
e o
rig
ina
lso
urc
e (
inve
stig
ato
r o
r tis
sue
of
ori
gin
) o
f th
is li
ne
(se
e N
ote
2).
129
130 Lynn
Fig. 1. Number of cell lines reported from Lepidoptera based on the information inTable 1, accumulative by decade.
2. Notes1. Table 1 includes more than 260 cell lines from various lepidopteran species pro-
viding a vast supply of material for research on baculoviruses. Although I havenot made an extensive literature search on virus susceptibilities of these cell lines(most of the details on viruses included in Table 1 were reported in the originalpublication describing the specific cell line or in one of Hink’s compilations [2–6] or the Granados and Hashimoto review [7]), approx 60% of these cell lines areknown to replicate one or more baculovirus. The designations used in Table 1are based on the original source of the virus as defined in Table 2. Over 100 linesare known to replicate the Autographa californica multiple nucleopoly-hedrovirus, which may surprise the majority of researchers using Sf-9, High Five,or Sf21AE cells with this virus as an expression vector.
2. The availability of some of these cell lines is unknown. Although a few insectcell lines are available through repositories (such as the American Type CultureCollection [ATCC], Manassas, VA, or the European Collection of Cell Cultures[ECACC], Health Protection Agency, Porton Down, Salisbury, Wiltshire),researchers interested in the use of most of these cell lines will need to obtainthem from other laboratories. If at all possible, this should be from the originalsource of the cells. Unfortunately, many of the earliest insect cell culturists areno longer active, making this somewhat problematic. However, the pool ofresearchers that have created new cell cultures is relatively small, so contact-ing one of the active researchers in the field will likely lead to a source if the cellline is still in existence.
Lepidopteran Insect Cell Lines 131
Table 2Baculoviruses Grown in Cell Culture
Original source and virus generaa Designation
Anagrapha falcifera NPV AnfaNPVAntheraea yamamai NPV AnyaNPVAnticarsia gemmatalis MNPV AgMNPVArtogeia rapae GV ArGVArtogeia rapae NPV ArNPVAutographa californica MNPV AcMNPVBombyx mori NPV BmNPVBuzura suppressaria NPV BusuNPVChoristoneura fumiferana MNPV CfMNPVChoristoneura murinana NPV ChmuNPVCydia pomonella GV CpGVDiapropsis watersii NPV DiwaNPVGalleria mellonella MNPV GmMNPVHelicoverpa armisgera NPV HearNPVHelicoverpa zea SNPV HzSNPVHyphantria cunea NPV HycuNPVLambdina fiscellaaria somniaria NPV LafiNPVLatoia viridissima NPV LaviNPVLymantria dispar MNPV LdMNPVMalacosoma disstria NPV MadiNPVMamestra brassicae MNPV MbMNPVOrgyia leucostigma NPV OrleNPVOrgyia pseudotsugata MNPV OpMNPVOrgyia pseudotsugata MNPV OpMNPVOrgyia pseudotsugata SNPV OpSNPVPhthorimaea operculella GV PhopGVPlutella xylostella GV PlxyGVPlutella xylostella MNPV PlxyMNPVRachiplusia ou MNPV RoMNPVSpilosoma imparilis NPV SpimNPVSpodoptera exigua MNPV SeMNPVSpodoptera frugiperda MNPV SfMNPVSpodoptera littoralis GV SpliGVSpodoptera littoralis NPV SpliNPVSpodoptera litura NPV SpltNPVThysanoplusia orichalcea NPV ThorNPVTrichoplusia ni SNPV TnSNPV
aThe viruses are NPV, nucleopolyhedrovirus; MNPV, multiple NPVs; SNPV, single NPVs;GV, granulovirus.
132 Lynn
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