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IIANEHil:HIMION 0EI:I:AAIAI: TMHMA BIOXHMEIAI: & BIOTEXNOAOrJAE HAOYT!!NOE 26 41221 AAPIEA THAIFAX. (2410) 565277/90 Email: [email protected] UNIVERSITY OF THESSAL Y BIOCHEMISTRY & BIOTECHNOLOGY DEPT PLOUTONOS 26 LARISSA, 41221-GREECE TEL/FAX. (302410) 565277/90 Email: [email protected] Demetrios Kouretas Professor CERTIFICATE OF ANALYSIS AND CLASSIFICATION OF AGRINIO COMPANY: ATMOS LAB COMMERCIAL NAME OF THE PRODUCT: AGRINIO TOTAL NUMBER: 01/AGRINIO 112 ANALYSIS (Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry) RESULTS table 1. percentage Limit of analysis substance (%w/v) (LOQ) classification ( m) propylene glycol 3,21 0,198 no glycerol 83,59 1,187 no water II ,6997 Acute toxicity 1 nicotine 1,5 0,1 Acute toxicity 3 Chronic toxicity 2 in aquatic organisms total 99,9997 Classification of the liquid Agrinio According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients of the mixture and according to EU Regulation 1272/2008 this product with 99.9991% composition, is classified as non hazardous for human health, environment and according to its physicochemical properties.

Atmos Lab's Tobacco Flavour Analysis EN

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Some of our famous tobacco flavour eliquid analysis datasheets. All of our eliquids are analyzed every month in Toxicology Laboratory of Medical School in University of Thessaly and Crete. TSNAs free, PAHs free, Sugar free, GMO free, gluten free, diacetyl free.

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Page 1: Atmos Lab's Tobacco Flavour Analysis EN

IIANEHil:HIMION 0EI:I:AAIAI: TMHMA BIOXHMEIAI: & BIOTEXNOAOrJAE HAOYT!!NOE 26 41221 AAPIEA

THAIFAX. (2410) 565277/90 Email: [email protected] ATJ!JtiTPTJ~ Kouptm~ Ku01Jy,Ttj~

UNIVERSITY OF THESSAL Y BIOCHEMISTRY & BIOTECHNOLOGY DEPT

PLOUTONOS 26 LARISSA, 41221-GREECE

TEL/FAX. (302410) 565277/90 Email: [email protected]

Demetrios Kouretas Professor

CERTIFICATE OF ANALYSIS AND CLASSIFICATION OF AGRINIO

COMPANY: ATMOS LAB COMMERCIAL NAME OF THE PRODUCT: AGRINIO TOTAL NUMBER: 01/AGRINIO 112

ANALYSIS (Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)

RESULTS

table 1.

percentage Limit of analysis

substance (%w/v) (LOQ) classification ( m)

propylene glycol 3,21 0,198 no

glycerol 83,59 1,187 no

water II ,6997 Acute toxicity 1

nicotine 1,5 0,1 Acute toxicity 3

Chronic toxicity 2 in aquatic organisms

total 99,9997

Classification of the liquid Agrinio

According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients of the mixture and according to EU Regulation 1272/2008 this product with 99.9991% composition, is classified as non hazardous for human health, environment and according to its physicochemical properties.

Page 2: Atmos Lab's Tobacco Flavour Analysis EN

NYTROZAMINE ANALYSIS

(Liquid Chromatography -Mass Spectrometry)

table 2. name

4-(Methylnitrosamino )-1-(3-pyridyl)-1-butanol 4'-(nitrosomethylamino )-1-(3-pyridyl)-1-butanone N-nitrosoanatabine N-nitrosoanabasine

RESULTS

concentration (ppm)

ND*

ND ND ND

(LOQ) (ppm)

0,01

0,01 0,05 0,01

POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS

(Gas Chromatography -Mass Spectrometry)

RESULTS

table 3. name Concentration (LOQ)

(ppm) (ppm)

acenaphtylene ND* 0,005 fluorene ND 0,010

phenanthrene ND 0,005 anthracene ND 0,006

pyrene ND 0,003 benzo(a)anthracene ND 0,003

chrysene ND 0,003 benzo(k)fluoranthene ND 0,060 benzo(b )fluoranthene ND 0,006 benzo(g,h,i)perylene ND 0,014

dibenzo(a,h)anthracene ND 0,021 indeno( 1 ,2,3-CD)pyrene ND 0,022

Page 3: Atmos Lab's Tobacco Flavour Analysis EN

Demetrios Kouretas, PhD

Professor

Biochemical Toxicology

Page 4: Atmos Lab's Tobacco Flavour Analysis EN

DANEnn:ntMION 0EI:I:AAIAI: TMHMA BIOXHMEIAI: & BIOTEXNOAOrlAI: DAOYTflNOI: 26 41221 AAPII:A

THA/FAX. (2410) 565277/90 Email: [email protected] AqJ.niTPil~ KouptTa~ KaO,yljTij~

UNIVERSITY OF THESSAL Y BIOCHEMISTRY & BIOTECHNOLOGY DEPT

PLOUTONOS 26 LARISSA, 41221-GREECE

TEL/FAX. (302410) 565277/90 Email: [email protected]

Demetrios Kouretas Professor

CERIFICATE OF ANALYSIS AND CLASSIFICATIONS OF APACHE

COMPANY: ATMOS LAB COMMERCIAL NAME OF THE PRODUCT: APACHE TOTAL NUMBER: 011 APACHE 112

ANALYSIS (Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)

RESULTS table 1.

substance persentage

Limit of analysis (LOQ)

( m)

propylene glycol

glycerol

water

nicotine

total

Classification of the liquid Apache

(%w/v)

4,05

84,93

9,7144

1,3

99,9944

0,198

I ,187

0,1

classification

no

no

Acute toxicity 1 Acute toxicity 3

Chronic toxicity 2 in aquatic organisms

According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients of the mixture and according to EU Regulation 1272/2008 this product with 99.9991 % composition, is classified as non hazardous for human health, environment and according to its physicochemical properties.

Page 5: Atmos Lab's Tobacco Flavour Analysis EN

NYTROZAMINE ANALYSIS

(Liquid Chromatography -Mass Spectrometry)

table 2. name

4-(Methylnitrosamino )-1-(3-pyridyl)-1-butanol 4'-(nitrosomethylarnino )-1-(3-pyridyl)-1-butanone N -nitrosoanatabine N -nitrosoanabasine

RESULTS

concentration (ppm)

ND*

ND

ND ND

(LOQ) (ppm)

0,01

0,01 0,05

0,01

POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS

(Gas Chromatography -Mass Spectrometry)

RESULTS

table 3. name conentration (LOQ)

(ppm) (ppm)

acenaphtylene ND* 0,005

fluorene ND 0,010 phenanthrene ND 0,005

anthracene ND 0,006 pyrene ND 0,003

benzo(a)anthracene ND 0,003

chrysene ND 0,003 benzo(k)fluoranthene ND 0,060 benzo(b )fluoranthene ND 0,006

benzo(g,h,i)perylene ND 0,014 dibenzo(a,h)anthracene ND 0,021

indeno( 1 ,2,3-CD )pyrene ND 0,022

Page 6: Atmos Lab's Tobacco Flavour Analysis EN

Demetrios Kouretas, PhD

Professor

Biochemical Toxicology

Page 7: Atmos Lab's Tobacco Flavour Analysis EN

nANEnt:!:::TIIMION 0EI:I:AAIAI: TMHMA BIOXHMEIAI: & BIOTEXNOAOriAI: nAOYT!!NOI: 26 41221 AAPII:A

TIIAIFAX. (2410) 565277/90 Email: [email protected] Al]lliJTP'l~ KouptTU~ Ka01]y1JT1~

UNIVERSITY OF THESSAL Y BIOCHEMISTRY & BIOTECHNOLOGY DEPT

PLOUTONOS 26 LARISSA, 41221-GREECE

TEL/FAX. (302410) 565277/90 Email: [email protected]

Demctrios Kouretas Professor

CERTIFICATE OF ANALYSIS AND CLASSIFICATON OF BARBARA

COMPANY: ATMOS LAB COMMERCIAL NAME OF THE PRODUCT: BARBARA TOTAL NUMBER: OJ/BARBARA /12

ANALYSIS (Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)

table 1.

substance

propylene glycol

glycerol

water

nicotine

total

RESULTS

percentage (%w/v)

3,67

80,19

15,0376

1,1

99,9976

Limit of analysis (LOQ) ( m)

0,198

I ,187

0,1

Classification of the liquid Barbara

classification

no

no

Acute toxicity 1 Acute toxicity 3

Chronic toxicity 2 in aquatic organisms

According to Greek regulation ( FEK 1214/B'/19.9.2002) , classification of particular ingredients of the mixture and according to EU Regulation 1272/2008 this product with 99.9991 % composition, is classified as non hazardous for human health, environment and according to its ph ysicochernical properties.

Page 8: Atmos Lab's Tobacco Flavour Analysis EN

NYTROZAMINE ANALYSIS

(Liquid Chromatography -Mass Spectrometry)

RESULTS

table 2. name concentration (LOQ)

(ppm) (ppm) 4-(Methylnitrosamino )-1-(3-pyridyl)-1-butanol ND* 0,01 4'-(nitrosomethylamino )-1-(3-pyridyl)-1-butanone ND 0,01 N-ni trosoanatabine ND 0,05 N -nitrosoanabasine ND 0,01

POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS

(Gas Chromatography -Mass Spectrometry)

RESULTS

Table 3. name concentration (LOQ)

(ppm) (ppm)

acenaphtylene ND* 0,005

fluorene ND 0,010 phenanthrene ND 0,005

anthracene ND 0,006

pyrene ND 0,003 benzo(a)anthracene ND 0,003

chrysene ND 0,003 benzo(k)fluoranthene ND 0,060 benzo(b )fluoranthene ND 0,006 benzo(g,h,i)perylene ND 0,014

dibenzo( a,h )anthracene ND 0,021

indeno( 1 ,2,3-CD)pyrene ND 0,022

Page 9: Atmos Lab's Tobacco Flavour Analysis EN

Demetrios Kouretas, PhD

Professor

Biochemical Toxicology

Page 10: Atmos Lab's Tobacco Flavour Analysis EN

fiANEOJI:TIIMION 0EI:I:AAIAI: TMHMA BIOXHMEIAI: & BIOTEXNOAOriAI: fiAOYT!!NOI: 26 41221 AAPII:A

THA/FAX. (2410) 565277/90 Email: [email protected] AIJfJJlTPll~ KouptTU~ KaOtJ'YIJTJl~

UNIVERSITY OF THESSAL Y BIOCHEMISTRY & BIOTECHNOLOGY DEPT

PLOUTONOS 26 LARISSA, 41221-GREECE

TEL/FAX. (302410) 565277/90 Email: [email protected]

Demetrios Kouretas Professor

CERTIFICATE OF ANALYSIS AND CLASSIFICATION OFBEBECA

COMPANY: ATMOS LAB

COMMERCIAL NAME OF PRODUCT: BEBECA TOTAL NUMBER: 01/BEBECA/12

ANALYSIS (Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)

table 1.

substance

methyl cyclopentenolone

ethyl maltol

ethyl vanillin

propylene glycol

glycerol

water

nicotine

total

RESULTS

Percentage (%w/v)

0,503

1,130

0,004

9,35

85,86

1,6522

1,5

99,9992

Limit of analysis (LOQ) ( m) 0,121

0,135

0,032

0,198

1,187

0,1

classification

no

Oral acute toxicity group 4

Skin irritation group 2

Eye irritation group 2

Special toxicity in target organs group 3

no

no

Acute toxicity 1 Acute toxicity 3

Chronic toxicity 2 in aquatic organisms

Page 11: Atmos Lab's Tobacco Flavour Analysis EN

Classification of the liquid Bebeca

According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients of the mixture and according to EU Regulation 1272/2008 this product with 99.9991% composition, is classified as non hazardous for human health, environment and according to its physicochemical properties

NYTROZAMINE ANALYSIS

(Liquid Chromatography -Mass Spectrometry)

table 2. name

4-(Methylnitrosamino )-1-(3-pyridyl)-1-butanol 4'-(nitrosomethylamino )-1-(3-pyridyl)-1-butanone N -nitrosoanatabine

N -nitrosoanabasine

RESULTS

concentration (ppm)

ND*

ND ND

ND

(LOQ) (ppm)

0,01

0,01 0,05 0,01

POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS

(Gas Chromatography -Mass Spectrometry)

RESULTS

table 3. name concentration (LOQ)

(ppm) (ppm)

acenaphtylene ND* 0,005

fluorene ND 0,010

phenanthrene ND 0,005

anthracene ND 0,006

pyrene ND 0,003

benzo(a)anthracene ND 0,003

chrysene ND 0,003

benzo(k)fluoranthene ND 0,060

benzo(b )fluoranthene ND 0,006

benzo(g,h,i )perylene ND 0,014

dibenzo(a,h)anthracene ND 0,021

indeno( 1 ,2,3-CD )pyrene ND 0,022

Page 12: Atmos Lab's Tobacco Flavour Analysis EN

Demetrios Kouretas, PhD

Professor

Biochemical Toxicology

Page 13: Atmos Lab's Tobacco Flavour Analysis EN

nANEniETHMION 0EEEAAIAE TMHMA BIOXHMEIAE & BIOTEXNOAOrtAE nAOYT!!NOE 26 41221 AAPIEA

THAIFAX. (2410) 565277/90 Email: [email protected] Al]Jl1JTP1]~ KouptTa~ Ka91)YlJTtj~

UNIVERSITY OF THESSAL Y BIOCHEMISTRY & BIOTECHNOLOGY DEPT

PLOUTONOS 26 LARISSA, 41221-GREECE

TEL/FAX. (302410) 565277/90 Email: [email protected]

Demetrios Kouretas Professor

CERTIFICATE OF ANALYSIS AND CLASSIFICATION OFCAMILA

COMPANY: ATMOS LAB COMMERCIAL NAME OF PRODUCT: CAMll..A TOTAL NUMBER: 011 CAMILA 112

ANALYSIS (Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)

RESULTS

Table 1.

Percentage Limit of analysis

substance (%w/v)

(LOQ) classification ( m)

Oral acute toxicity group 4

Skin irritation group 2

ethyl vanillin 0,001 0,032 Eye irritation group 2

Special toxicity in target organ group 3

propylene glycol 15,52 0,198 no

gl~cerol 80,70 1,187 no

water 2,2773 Acute toxicity 1

nicotine 1,5 0,1 Acute toxicity 3

Chronic toxicity 2 in aguatic organisms

total 99,9983

Page 14: Atmos Lab's Tobacco Flavour Analysis EN

Classification of the liquid CAMILA

According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients of the mixture and according to EU Regulation 1272/2008 this product with 99.9991% composition, is classified as non hazardous for human health, environment and according to its physicochemical properties.

NYTROZAMINE ANALYSIS

(Liquid Chromatography -Mass Spectrometry)

table 2. name

4-(Methylnitrosamino )-1-(3-pyridyl)-1-butanol 4'-(nitrosomethylarnino )-1-(3-pyridyl)-1-butanone N -nitrosoanatabine N -nitrosoanabasine

RESULTS

concentration (ppm)

ND*

ND ND ND

(LOQ) (ppm)

0,01

0,01 0,05 0,01

POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS

(Gas Chromatography -Mass Spectrometry)

RESULTS

total 3. name concentration (LOQ)

(ppm) (ppm)

acenaphtylene ND* 0,005 fluorene ND 0,010

phenanthrene ND 0,005

anthracene ND 0,006 pyrene ND 0,003

benzo(a)anthracene ND 0,003

chrysene ND 0,003

benzo(k)fluoranthene ND 0,060

benzo(b )fluoranthene ND 0,006

benzo(g,h,i )pery lene ND 0,014

dibenzo(a,h)anthracene ND 0,021

indeno( 1 ,2,3-CD )pyrene ND 0,022

Page 15: Atmos Lab's Tobacco Flavour Analysis EN

Demetrios Kouretas, PhD

Professor

Biochemical Toxicology

Page 16: Atmos Lab's Tobacco Flavour Analysis EN

flANEllll:THMION 0EI:I:AAIAI: TMHMA BIOXHMEIAI: & BIOTEXNOAOriAI: nAOYT!!NOI: 26 41221 AAPII:A

THAIFAX. (2410) 565277/90 Email: [email protected] AlJJltJTPlJ~ Kollptm~ Ko01JY1JTtJ~

UNIVERSITY OF THESSAL Y BIOCHEMISTRY & BIOTECHNOLOGY DEPT

PLOUTONOS 26 LARISSA, 41221-GREECE

TEL/FAX. (302410) 565277/90 Email: [email protected]

Demetrios Kouretas Professor

CERTIFICATE OF ANALYSIS AND CLASSIFICATION OFKAPNOS

COMPANY: ATMOS LAB COMMERCIAL NAME OF THE PRODUCT: KAPNOS TOTAL NUMBER: 0 1/KAPNOS/ 12

ANALYSIS (Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)

table 1.

substance

propylene glycol

glycerol

water

nicotine

total

RESULTS

percentage (%w/v)

3,28

89,61

5,6996

1,4

99,9896

Limit of analysis (LOQ) ( m) 0,198

1,187

0,1

Classification of the liquid KAPNOS

classification

no

no

Acute toxicity 1 Acute toxicity 3

Chronic toxicity 2 in aquatic organisms

According to Greek regulation ( FEK 1214/B '119 .9 .2002) , classification of particular ingredients of the mixture and according to EU Regulation 1272/2008 this product with 99.9991 % composition, is classified as non hazardous for human health, environment and according to its physicochemical properties.

Page 17: Atmos Lab's Tobacco Flavour Analysis EN

NYTROZAMINE ANALYSIS

(Liquid Chromatography -Mass Spectrometry)

RESULTS

table 2. name concentration (LOQ)

(ppm) (ppm) 4-(Methylnitrosamino )-1-(3-pyridyl)-1-butanol ND* 0,01 4'-(nitrosomethylamino )-1-(3-pyridyl)-1-butanone ND 0,01

N -nitrosoanatabine ND 0,05

N -nitrosoanabasine ND 0,01

POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS

(Gas Chromatography -Mass Spectrometry)

RESULTS

table 3. name concentration (LOQ)

(ppm) (ppm)

acenaphtylene ND* 0,005 fluorene ND 0,010

phenanthrene ND 0,005

anthracene ND 0,006

pyrene ND 0,003 benzo(a)anthracene ND 0,003

chrysene ND 0,003 benzo(k)fluoranthene ND 0,060 benzo(b )fluoranthene ND 0,006 benzo(g,h,i )perylene ND 0,014

dibenzo(a,h)anthracene ND 0,021

indeno( 1 ,2,3-CD)pyrene ND 0,022

Page 18: Atmos Lab's Tobacco Flavour Analysis EN

Demetrios Kouretas, PhD

Professor

Biochemical Toxicology