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H A N D B O O K O F

MicrobiologicalMedia for theExamination of Food

By

RONALD M. ATLAS

S E C O N D E D I T I O N

Boca Raton London New York

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Library of Congress Cataloging-in-Publication Data

Atlas, Ronald M. 1946-Handbook of microbiological media for the examination of food / by Ronald M. Atlas.--2nd ed.

p. cm.Includes bibliographical references and index.ISBN 0-8493-3561-2 (alk. paper)1. Food--Microbiology--Laboratory manuals. 2. Culture media (Biology)--Laboratory manuals. I. Title:

Microbiological medica for the examination of food. II. Title.

QR115.A84 2006664'.001'579--dc22 2005054896

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Preface

Increased emphasis on the microbiological safety of foods has led to greater testing of foods for poten-tially pathogenic microorganisms. Careful quality control in the food industry has become essential for preventing outbreaks of foodborne disease and for controlling food spoilage. Many foods are routinely examined for the presence of disease-causing and food spoilage microorganisms. Many of these qual-ity control procedures involve the cultivation of microorganisms using a variety of media that are described in the second edition of the Handbook of Microbiological Media for the Examination of Food.

Given that an estimated 14 million cases of food-borne disease with known microbial pathogens occur every year in the United States alone, the Food and Drug Administration and U.S. Department of Agriculture have been implementing new regula-tions and guidance for the microbial testing of a variety of foods. Similarly, Europe and other regions of the developed world are implementing stricter oversight of food safety to ensure that foods are free of potentially dangerous pathogens, e.g., Salmonellaspp, Vibrio spp., Listeria monocytogenes, Campylo-bacter jejuni, etc.

Emerging diseases caused by foodborne pathogens, such as Escherichia coli O157:H7, that have high mortality rates have raised special concerns. This has encouraged the development of new media for the cultivation of bacteria, fungi, and viruses that can aid in the rapid detection of microorganisms in foods and the protection of the food supply. Many of these new media include chromogenic or fluoro-genic substrates that permit the specific rapid detec-tion of pathogens of concern.

The second edition of the Handbook of Microbiolog-ical Media for the Examination of Food provides descriptions of the media used to cultivate microor-ganisms from foods. Almost 1,400 media are described in the Handbook, including all the media recommended by the Food and Drug Administration for the detection of microorganisms in foods as well as media used elsewhere in the world. The Hand-book includes descriptions of the media used to cul-tivate microorganisms used for food production as well as those that are important for quality control, i.e., the cultivation of potential pathogens and food-spoilage microorganisms.

The second edition of the Handbook of Microbiolog-ical Media for the Examination of Food compiles in one place in a consistent style the formulations,

methods of preparation, and uses of each medium. It lists the common synonyms for the various media and the commercial sources when they are available. The index provides a convenient tool for finding the media by names and application for the detection of specific microorganisms of interest.

Importantly, the Handbook of Microbiological Media for the Examination of Food includes descriptions of expected results as they apply to microorganisms of importance for the examination of foods. The compositions of various media can be compared so that alternate media can be used. Simi-lar or identical media described by different names that actually have the same formulations can be readily identified.

Using the second edition of the Handbook of Micro-biological Media for the Examination of Foodshould save time and effort for anyone cultivating microorganisms. The media are organized alphabeti-cally. Each medium includes the composition, instructions for the preparation, and uses of each of the media.

The information needed to prepare media for the cultivation of microorganisms can easily be found in the Handbook. It should be a valuable resource for anyone working in the area of food microbiology.

About the AuthorRonald M. Atlas is Professor of Biology at the Uni-versity of Louisville. He received his B.S. from the State University of New York at Stony Brook in 1968 and his M.S. and Ph.D. from Rutgers in 1970 and 1972, respectively. Dr. Atlas has received a number of honors including: The University of Louisville Excellence in Research Award, the Johnson and Johnson Fellowship for Biology, and the ASM Award in Applied and Environmental Microbiology.

He has taught a variety of courses in microbiology including food and industrial microbiology and has authored several microbiology textbooks. He has conducted studies on the use of gene probes and the polymerase chain reaction for the detection of pathogens and indicator bacteria in water and food. He has served on the editorial boards of Applied and Environmental Microbiology, BioScience, Bio-techniques, and Journal of Industrial Microbiology. He is editor of CRC Critical Reviews in Microbiol-ogy. He also has served as President of the Ameri-can Society for Microbiology.

Introduction to Food Microbiology ......................1Food- and Waterborne Pathogens ............................1Food Spoilage ..........................................................6Microbiological Production of Food .......................7A-1 Medium...........................................................11AAM Medium........................................................11Abeyta-Hunt Bark Agar.........................................11ABY Agar ..............................................................12AC Agar.................................................................12AC Broth................................................................12Acetamide Agar .....................................................12Acetamide Agar .....................................................13Acetamide Broth ....................................................13Acetamide Medium................................................13Acetate Agar ..........................................................13Acetate Broth .........................................................14Acetate Differential Agar.......................................14Acetobacter Agar ...................................................14Acetobacter/Gluconobacter Agar ..........................15Acetobacter Medium..............................................15Acetobacter xylinum Medium................................15Acetogen Medium..................................................15Achromobacter Choline Medium ..........................16Achromobacter Choline Medium, Modified..........16Achromobacter Medium ........................................16Achromobacter Medium ........................................17Acid Broth..............................................................17Acid Egg Medium..................................................17Acid Products Test Broth .......................................17Acid Tomato Broth ................................................17Acidic Tomato Medium for Leuconostoc ..............18Acidophilic Bacillus stearothermophilus Agar .....18Acidophilic Bacillus stearothermophilus Broth ....18Actidione Agar.......................................................18AE Sporulation Medium, Modified .......................18Aeromonas Differential Agar.................................19Aeromonas hydrophila Medium ............................19Aeromonas Medium...............................................20AFPA......................................................................20Agar Medium for Differential

Enumeration of Lactic Streptococci ................20Agar Medium P......................................................21AK Agar No. 2.......................................................21AKI Medium..........................................................21Alcal Mannose Medium.........................................22Alcaligenes Agar....................................................22Alcaligenes Medium ..............................................22Alcaligenes N5 Medium ........................................23Alcaligenes NA YE Medium .................................23Alcaligenes NB YE Agar .......................................23Alcaligenes NB YE Broth......................................23Alcaligenes NB YE Medium .................................23Alginate Utilization Medium.................................23

Alkaline Bacillus Medium.....................................24Alkaline Peptone Agar...........................................24Alkaline Peptone Salt Broth ..................................24Alkaline Peptone Water .........................................24Alkaline Peptone Water .........................................25Alkaline Polypectate Agar.....................................25Alkaline Starch Agar .............................................25Alkaline Xylan Agar..............................................25Alkalophile Medium..............................................25Alkvisco Medium ..................................................25Allantoin Agar .......................................................26ALOA Medium......................................................26ALOA Medium......................................................27Amino Acid Assay Medium ..................................28Anaerobe Agar.......................................................28Anaerobe Agar.......................................................29Anaerobe Medium .................................................29Anaerobic Agar......................................................29Anaerobic Agar......................................................30Anaerobic Broth.....................................................30Anaerobic CNA Agar ............................................30Anaerobic Egg Yolk Agar......................................30Anaerobic Egg Yolk Agar......................................31Anaerobic TVLS Medium .....................................31Andersens Pork Pea Agar .....................................31Andrades Broth.....................................................32Andrades Carbohydrate Broth

and Indicator....................................................33Anthranilic Acid Medium, Revised.......................33Antibiotic Medium 1..............................................34Antibiotic Medium 1 with Tetracycline.................34Antibiotic Medium 1 with Tetracycline,

Streptomycin, and Chloramphenicol...............34Antibiotic Medium 2..............................................34Antibiotic Medium 3..............................................35Antibiotic Medium 3 Plus......................................35Antibiotic Medium 4..............................................35Antibiotic Medium 5..............................................35Antibiotic Medium 6..............................................35Antibiotic Medium 7..............................................36Antibiotic Medium 8..............................................36Antibiotic Medium 9..............................................36Antibiotic Medium 10............................................36Antibiotic Medium 11............................................36Antibiotic Medium 12............................................37Antibiotic Medium 13............................................37Antibiotic Sulfonamide

Sensitivity Test Agar .......................................37Antibiotic Sulfonamide

Sensitivity Test Agar .......................................37AOAC Letheen Broth ............................................38Aolpha Medium.....................................................38Apple Juice Yeast Extract Medium .......................39

Table of Contents

APT Agar ...............................................................39APT Broth ..............................................................39Arginine Broth .......................................................40Arginine Broth with NaCl......................................40Arginine Glucose Slants ........................................40Armstrong Fusarium Medium...............................40Ascospore Agar......................................................41ASLA Agar ............................................................41Asparaginate Glycerol Agar ..................................41Asparagine Broth ...................................................41Aspergillus Differential Medium ...........................41ATB Acid Tomato Broth........................................41Azide Blood Agar ..................................................42Azide Blood Agar with Crystal Violet...................42Azide Broth............................................................42Azide Medium .......................................................42B12 Culture Agar, USP...........................................43B12 Inoculum Broth, USP ......................................43Bacillus Agar .........................................................43Bacillus Agar, Modified.........................................43Bacillus Agar, 1/4 Strength....................................43Bacillus Broth, 1/4 Strength...................................44Bacillus cereus Medium ........................................44Bacillus cereus Selective Agar Base......................44Bacillus coagulans Medium ..................................44Bacillus fastidiosus Agar .......................................45Bacillus fastidiosus Medium..................................45Bacillus Medium....................................................45Bacillus stearothermophilus Broth ........................46Bacillus stearothermophilus

Defined Broth ..................................................46Bacillus stearothermophilus

Sporulation Broth ............................................46Bacillus thermoantarcticus Medium......................46Bacillus Xylose Salts .............................................47BAGG Broth ..........................................................47Baird-Parker Agar..................................................48Baird-Parker Agar, Supplemented .........................48Balamuth Medium .................................................48Baird-Parker Medium ............................................49Basal Synthetic Medium........................................49Base Layer Agar with

Nutrient Overlay Agar.....................................49BC Motility Medium ............................................50BCM Bacillus cereus

Group Plating Medium ...................................50BCM O157:H7(+) Plating Medium ......................50BCP Azide Broth ...................................................50BCP D Agar ...........................................................51BCP DCLS Agar....................................................51Beef Extract Broth .................................................51Beef Infusion Agar.................................................51BG Sulfa Agar .......................................................51Bicarbonate Agar ...................................................52Bile Esculin Agar ..................................................52Bile Oxalate Sorbose Broth ..................................53

Bile Salts Brilliant Green Starch Agar ..................53Bile Salts Gelatin Agar .........................................54Biosynth Chromogenic Medium

for Listeria monocytogenes .............................54Biotin Assay Medium ...........................................54Bismuth Sulfite Agar ............................................54Bismuth Sulfite Agar Wilson and Blair ................55Bismuth Sulfite Broth ...........................................55BL Agar ................................................................55Blood Agar ............................................................56Blood Agar Base ...................................................56Blood Agar Base ...................................................57Blood Agar with 1% NaCl ....................................57Blood Agar Base No. 2..........................................57Bolton Broth ..........................................................57Bouillon Medium...................................................58Bovine Albumin Tween 80 Medium,

Ellinghausen and McCullough, Modified .......58Bovine Albumin Tween 80

Semisolid Medium, Ellinghausen and McCullough, Modified .............................58

Bovine Serum Albumin Tween 80 Agar ...............59Bovine Serum Albumin Tween 80 Broth .............59Bovine Serum Albumin Tween 80 Soft Agar .......60Brain Heart Infusion Agar ....................................60Brain Heart Infusion Agar 0.7% ...........................61Brain Heart Infusion Broth ....................................61Brewer Anaerobic Agar........................................ 62Brewer Thioglycolate Medium..............................62Briggs Liver Tomato Broth...................................62Brilliant Green Agar .............................................62Brilliant Green Agar, Modified .............................62Brilliant Green Agar, Modified .............................63Brilliant Green Agar with Sulfadiazine ................63Brilliant Green Bile Agar ......................................63Brilliant Green 2%-Bile Broth, Fluorocult ............64Brilliant Green Bile Broth ....................................64Brilliant Green Broth ............................................64Brilliant Green Lactose Bile Broth .......................64Brilliant Green Bile Broth with MUG ..................65Brilliant Green Phenol Red Agar ..........................65Bromcresol Purple Broth ......................................65Bromcresol Purple Broth with NaCl .....................66Bromcresol Purple Dextrose Broth .......................66Bromthymol Blue Agar ........................................66Brucella Agar ........................................................67Brucella Agar with Vitamin K1 .............................67Brucella Albimi Broth with 0.16% Agar...............67Brucella Albimi Broth with 0.16% Agar

and 1% Glycine ..............................................67Brucella Anaerobic Blood Agar ...........................68Brucella Broth ......................................................68Brucella Broth with 0.16% Agar ..........................68Brucella Medium Base .........................................68Brucella Medium, Selective .................................68Brucella Selective Medium .................................. 69

Brucella Semisolid Medium with Cysteine ..................................................69

Brucella Semisolid Medium with Glycine ...................................................69

Brucella Semisolid Medium with NaCl ................70Brucella Semisolid Medium with Nitrate .............70Buffered Enrichment Broth ...................................70Buffered Peptone Water ........................................71CAL Broth ............................................................71Campylobacter Agar, Blasers.............................. 71Campylobacter Agar, Skirrows ...........................71Campylobacter Blood-Free Agar Base

Modified .........................................................72Campylobacter Blood-Free Selective Agar ..........72Campylobacter Charcoal Differential Agar ..........72Campylobacter Enrichment Broth ........................73Campylobacter Enrichment Broth ........................74Campylobacter Enrichment Broth ........................75Campylobacter Enrichment Broth ........................76Campylobacter fecalis Medium ............................76Campylobacter fetus Medium ...............................77Campylobacter fetus Selective Medium ...............77Campylobacter Isolation Agar A ..........................78Campylobacter Isolation Agar B ..........................78Campylobacter Medium .......................................78Campylobacter Selective

Medium, Blaser-Wang ....................................79Campylobacter Selective

Medium, Butzlers ..........................................80Campylobacter Selective

Medium, Karmalis .........................................80Campylobacter Selective Medium, Prestons .......81Carbohydrate Fermentation Broth ........................81Casamino Acids Yeast Extract Broth ....................82Cary and Blair Transport Medium........................ 82Casamino Acids Yeast Extract Broth ....................82Casamino Acids Yeast

Extract Lincomycin Medium ..........................82Casamino Acids Yeast

Extract Salts Broth, Gorbach ..........................83CCY Modified Medium ........................................83Cefsulodin Irgasan Novobiocin Agar ...................83Cell Growth Medium ............................................84Cellobiose Polymyxin Colistin Agar ....................85Cellobiose Polymyxin B

Colistin Agar, Modified ..................................85Cellulase Solution .................................................86Cereal Agar ...........................................................86Cetrimide Agar, Non-USP ....................................86Cetrimide Agar, USP ............................................86Chapman Stone Agar ............................................87Cheese Agar ..........................................................87Cherry Agar, CBS Formula ..................................87China Blue Lactose Agar ......................................87CHO Medium Base ...............................................87Cholera Medium TCBS ........................................ 88

Chopped Liver Broth ............................................ 88Chopped Meat Agar ..............................................88Chopped Meat Broth .............................................88Chopped Meat Carbohydrate Medium .................89Christensen Agar ..................................................89Christensen Citrate Agar .......................................89Christensen Citrate Agar, Modified.......................90Christensen Citrate Sulfide Medium ....................90Christensens Urea Agar .......................................90Christensens Urea Agar with NaCl .....................90CHROMagar E. coli .............................................91CHROMagar ECC ................................................91CHROMagar Listeria ...........................................91CHROMagar O157................................................91CHROMagar Salmonella ......................................91CHROMagar Staph. aureus ..................................92CHROMagar Vibrio ..............................................92Chromogenic Bacillus cereus Agar ......................92Chromogenic E. coli/Coliform Medium ...............92Chromogenic Enterobacter sakazakii

Agar, DFI Formulation ...................................93Chromogenic Listeria Agar ..................................93Chromogenic Salmonella Esterase Agar ..............93Chromogenic Substrate Broth ...............................93CIN Agar ..............................................................94Clostridia Medium ................................................94Clostridium acidurici Medium .............................94Clostridium aerotolerans Medium ....................... 95Clostridium Alginate Medium ..............................95Clostridium aminobutyricum Medium .................95Clostridium aminobutyricum Medium .................96Clostridium botulinum Isolation Agar .................. 96Clostridium CK Medium ...................................... 97Clostridium difficile Agar .....................................97Clostridium perfringens Agar, OPSP ...................98Clostridium Selective Agar ...................................98Coagulase Agar Base............................................ 98Coagulase Mannitol Agar .....................................99Coletsos Medium ..................................................99Coletsos Selective Medium ..................................99Coli ID .................................................................100Coliform Agar, Chromocult.................................100Coliform Agar ES, Chromocult ...........................100Coliform Medium ................................................101Coliform Medium, Modified ...............................101Coliform Medium, Modified ...............................102Colonization Medium ..........................................102Congo Red Agar ..................................................102Congo Red Agar ..................................................103Congo Red BHI Agarose Medium ......................104Congo Red Magnesium Oxalate Agar.................104Conradi Drigalski Agar........................................105Cooke Rose Bengal Agar.....................................105Cooked Meat Medium .........................................105Cooked Meat Medium, Modified ........................106Cornmeal Agar.....................................................106

Cornmeal Agar.....................................................106Cornmeal Agar with Dextrose .............................107Cornmeal and V8 Juice Agar...............................107Cornmeal Agar, Quarter-Strength........................107Creatinine/NMH Medium....................................107CreDm1 Medium .................................................108Crossley Milk Medium ........................................109Crystal Violet Agar ..............................................109Crystal Violet Pectate Medium ............................109CS Vitamin B12 Agar ..........................................110CYA Agar with Arginine

and p-Aminobenzoic Acid.............................110Czapek Dox Agar, Modified................................110Czapek Dox Broth ...............................................110Czapek Dox Liquid Medium, Modified .............. 111Czapek Solution Agar ..........................................111Czapek Solution Agar with Sucrose .................... 111Czapek Yeast Autolysate Agar ............................ 111Czapek Yeast Extract Agar .................................. 111Czapek Yeast Extract Agar ..................................112DCLS Agar ..........................................................112Decarboxylase Basal Medium .............................112Decarboxylase Basal Medium with NaCl............113Decarboxylase Base, Mller ................................113Decarboxylase Medium Base, Falkow ................113Decarboxylase Medium, Ornithine Modified......113Demi-Fraser Broth ...............................................114Deoxycholate Agar ..............................................114Deoxycholate Citrate Agar ..................................114Deoxycholate Citrate Agar ..................................115Deoxycholate Citrate Agar, Hynes ......................115Deoxycholate Lactose Agar.................................115Dextrose Agar ......................................................116Dextrose Agar ......................................................116Dextrose Tryptone Agar ......................................116Dextrose Tryptone Broth .....................................116Dextrose Tryptone Broth .....................................117Dichloran 18% Glycerol Agar .............................117Dichloran-Glycerol Agar .....................................117Dichloran Rose Bengal

Chloramphenicol Agar ..................................117Differential Broth for Lactic Streptococci ...........118DNase Agar..........................................................118DNase Test Agar ..................................................118DNase Test Agar with Methyl Green...................118DNase Test Agar with Toluidine Blue .................119Dorset Egg Medium.............................................119Double-Strength Crude

Lactobacillus Medium...................................119Double-Strength Crude Medium

for Lactobacillus............................................119Doyle and Roman Enrichment Medium ..............120DRBC Agar..........................................................120Duncan-Strong

Sporulation Medium, Modified.....................121E Agar ..................................................................121

Eagles Minimal Essential Medium with Earles Salts and Nonessential Amino Acids .....................121

EB Motility Medium............................................122EC Broth ..............................................................122EC Broth with MUG............................................122EC Medium, Modified with Novobiocin.............122ECD Agar, Fluorocult..........................................123EE Broth ..............................................................123EE Broth, Mossel.................................................123Egg Yolk Agar .....................................................124Egg Yolk Agar, Modified.....................................124Egg Yolk Emulsion ..............................................124Egg Yolk Emulsion, 50%.....................................125Egg-Yolk Tellurite Emulsion 20%.......................125Eijkman Lactose Medium....................................125Elliker Agar .........................................................125Elliker Agar .........................................................126Elliker Broth ........................................................126Elliker Lactose Broth...........................................126EMB Agar............................................................126EMB Agar Base...................................................127EMB Agar, Modified...........................................127Endo Agar............................................................127Endo Agar............................................................128Endo Agar, LES...................................................128Endo Broth...........................................................128Enriched Nutrient Agar........................................129Enriched Nutrient Broth ......................................129Enrichment Broth

for Aeromonas hydrophila.............................129Enrichment Broth, pH 7.3....................................129Enrichment Broth, pH 7.3....................................130Enrichment Broth, pH 7.3 with Pyruvate ............130Enrichment Broth, pH 7.3 with Pyruvate ............131Entamoeba dispar Axenic Culture Medium........131Entamoeba Medium.............................................132Enteric Fermentation Base...................................132Enterobacter Medium..........................................133Enterococci Broth, Chromocult ...........................133Enterococci Confirmatory Agar ..........................133Enterococci Confirmatory Broth .........................133Enterococci Presumptive Broth ...........................134Enterococcosel Agar............................................134Enterococcosel Broth...........................................134Enterococcus Agar...............................................134Esculin Agar ........................................................135Esculin Agar, Modified CDC ..............................135Esculin Broth .......................................................135Esculin Iron Agar.................................................135Esculin Mannitol Agar.........................................135ETGPA.................................................................136Ethyl Violet Azide Broth .....................................136Ethyl Violet Azide Broth .....................................137Fay and Barry Medium........................................137FC Agar ...............................................................137

FC Agar................................................................138FC Broth ..............................................................138Fecal Coliform Agar, Modified ...........................138Fecal Coliform Agar, Modified ...........................139Fermentation Basal Medium................................139Fermentation Broth ..............................................139Fermentation Medium..........................................140FGTC Agar...........................................................140Fish Peptone Agar................................................140Fish Peptone Broth...............................................140Flavobacterium Medium .....................................141Flavobacterium Medium .....................................141Fluid Sabouraud Medium ....................................141Fluid Thioglycolate Agar.....................................141Fluid Thioglycolate Agar

with Calcium Carbonate ................................142Fletcher Medium..................................................142Fletcher Medium with Fluorouracil .....................142Fletchers Semisolid Medium..............................142FLN Medium .......................................................142Flo Agar ...............................................................143Fluid Thioglycolate Agar

with Calcium Carbonate ................................143Fluid Thioglycolate Medium ...............................143Fluid Thioglycolate Medium

with Beef Extract...........................................143Fluid Thioglycolate Medium with K Agar ..........144Fluid Thioglycolate Medium

with Rabbit Serum.........................................144Fluid Thioglycolate Medium

without Glucose or Eh Indicator ....................144Fluorescent Pectolytic Agar.................................144Fluorocult E. coli O157:H7 Agar ........................145FN Medium..........................................................145Folic Acid Agar ...................................................145Folic Acid Assay Medium ...................................146FPA Medium........................................................146Fraser Broth .........................................................146Fraser Secondary Enrichment Broth....................147Freezing Medium.................................................147G Medium............................................................147GBNA Medium....................................................148Gelatin Agar.........................................................148Gelatin Infusion Broth .........................................148Gelatin Medium ...................................................148Gelatin Phosphate Salt Agar ................................149Gelatin Phosphate Salt Broth...............................149Gelatin Salt Agar .................................................149Gelatinase Test Medium ......................................149Gentamicin Sulfate Solution................................150Giolitti-Cantoni Broth..........................................150Gluconate Peptone Broth .....................................150Glucose Agar with 25% Glucose.........................150Glucose Phosphate Broth.....................................150Glucose Salt Teepol Broth ...................................151Glucose Salts Medium.........................................151

Glucose Yeast Extract Agar .................................151Glucose Yeast Extract

Peptone Agar with 2% Glucose ....................151Glucose Yeast Extract Peptone Medium .............151Glucose Yeast Medium

with Calcium Carbonate................................152Glutamate Medium ..............................................152Glutamine Medium..............................................152Glutarate Medium................................................152GN Broth, Hajna..................................................153Green Yeast and Mold Broth ...............................153GSP Agar .............................................................153Gum Tragacanth Gum Arabic Medium ...............154GV Medium.........................................................154H Agar .................................................................155H Broth ................................................................155H Medium............................................................155H Top Agar ..........................................................155Halophile Medium...............................................155Halophilic Agar ...................................................156Halophilic Broth ..................................................156Halophilic Nutrient Agar .....................................156Halophilic Synthetic Medium..............................156Hams F-10 Medium............................................156Heart Infusion Agar .............................................157Heart Infusion Agar with NaCl............................158Heart Infusion Broth ............................................158Heart Infusion Broth with NaCl ..........................158Hektoen Enteric Agar ..........................................158Hemorrhagic Coli Agar .......................................159Hemorrhagic Coli Agar with MUG.....................159HHD Medium ......................................................159HiCrome Aureus Agar Base ................................159HiCrome Coliform Agar......................................160HiCrome Coliform Agar with Novobiocin..........160HiCrome E. coli Agar A ......................................161HiCrome E. coli Agar B ......................................161HiCrome ECC Agar.............................................161HiCrome ECC Selective Agar .............................161HiCrome ECC Selective Agar .............................162HiCrome ECC Selective Agar .............................162HiCrome Enterococci Broth ................................163HiCrome Listeria Agar Base, Modified...............163HiCrome MacConkey-Sorbitol Agar...................163HiCrome MacConkey-Sorbitol Agar

with Tellurite-Cefixime Supplement .............163HiCrome M-CP Agar Base..................................164HiCrome MS.O157 Agar.....................................164HiCrome MS.O157 Agar

with Tellurite .................................................165HiCrome OGYE Agar Base.................................165HiCrome Rapid Coliform Broth ..........................165HiCrome Rapid Enterococci Agar.......................166HiCrome Salmonella Agar ..................................166HL Agar ...............................................................166Horie Arabinose Ethyl Violet Broth ....................167

HP 101 Halophile Medium..................................167HP Medium..........................................................167HPC Agar.............................................................167Hugh-Leifsons Glucose Broth ............................168HY Agar for Flavobacterium ..............................168HY Medium for Flavobacterium .........................168HYA Agar ............................................................168Hydroxybenzoate Medium ..................................168Hydroxybenzoic Acid Medium ...........................169IE Medium ...........................................................170Imidazole Utilization Medium.............................170Indole Medium.....................................................171Indole Medium, CDC ..........................................171Indole Nitrite Medium .........................................171Infection Medium ................................................171Infusion Broth ......................................................171Inositol Brilliant Green Bile Salts Agar...............172Inositol Gelatin Deeps .........................................172Irgasan Ticarcillin Chlorate Broth .......................172Iron Agar, Lyngby................................................172Iron Milk Medium ...............................................173Iron Milk Medium, Modified...............................173IsoVitaleX Enrichment ........................................173J Agar...................................................................173J Broth..................................................................173Jordans Tartrate Agar..........................................173Kado's Agar .........................................................174Kanamycin Esculin Azide Agar ..........................174Kanamycin Esculin Azide Broth .........................174KC Bottom Agar..................................................174KC Broth..............................................................175KC Top Agar........................................................175KCN Broth...........................................................175KF Streptococcus Agar ........................................175KF Streptococcus Agar ........................................176KF Streptococcus Broth .......................................176Kim-Goepfert Agar..............................................176Kings B Medium.................................................177Kings Medium A ................................................177Kings Medium B.................................................177Kings O/F Basal Medium ...................................177Kleb Agar.............................................................177Kligler Iron Agar .................................................178Kligler Iron Agar .................................................179Kligler Iron Agar with NaCl................................179KM Agar ..............................................................179Korthof Medium ..................................................179Korthof Medium, Modified .................................180Koser Citrate Broth..............................................180Kranep Agar Base................................................180Krebs Yeast Lactate Medium..............................180Kunkee Medium ..................................................181KYE Agar ............................................................181L Agar ..................................................................181L Medium ............................................................181L Medium for Salmonella....................................181

L15 Medium, Modified Leibovitz .......................182Lab-Lemco Agar..................................................182Lab-Lemco Broth.................................................182Lactic Acid Bacteria Broth ..................................182Lactic Acid Bacteria Medium..............................183Lactic Agar for Yogurt Bacteria, Modified..........183Lactic Bacteria Broth...........................................183Lactic Streak Agar ...............................................184Lactobacilli Agar, AOAC ....................................184Lactobacilli Broth, AOAC...................................184Lactobacilli MRS Broth.......................................184Lactobacilli MRS Broth with Cysteine................185Lactobacilli MRS Broth with Ethanol .................185Lactobacillus bulgaricus Agar ............................185Lactobacillus Chloramphenicol Agar 1...............185Lactobacillus Chloramphenicol Broth 1..............186Lactobacillus Chloramphenicol Medium 2 .........186Lactobacillus Heteroferm Screen Agar ...............186Lactobacillus Heteroferm Screen Broth ..............186Lactobacillus Medium.........................................187Lactobacillus Medium II .....................................188Lactobacillus Medium III ....................................188Lactobacillus Medium IV....................................188Lactobacillus 8664 Medium................................188Lactobacillus Orotic Acid Medium.....................188Lactobacillus Selection Oxgall Agar...................189Lactose Broth.......................................................189Lactose Egg Yolk Milk Agar ...............................189Lactose Gelatin Medium......................................190Lactose Ricinoleate Broth....................................190Lauryl Sulfate Broth ............................................190Lauryl Sulfate Broth, Fluorocult..........................190Lauryl Sulfate Broth with MUG..........................191Lauryl Tryptose Broth .........................................191Lauryl Tryptose Broth with MUG.......................191Lauryl Tryptose Mannitol

Broth with Tryptophan ..................................191LB Agar ...............................................................192LB Medium..........................................................192LB Medium for 1776 ........................................192LBE Medium .......................................................192LBS Agar .............................................................193LBS Broth............................................................193Lead Acetate Agar ...............................................193Lecithin Lipase Anaerobic Agar..........................193Lees Agar............................................................194Leptospira Medium .............................................194Leptospira Medium, EMJH.................................194Leptospira Medium, Modified.............................194Leptospira Protein-Free Medium ........................195Letheen Agar, Modified.......................................195Letheen Broth, Modified .....................................196Leuconostoc Medium...........................................196Leuconostoc oenos Medium ................................196Levine EMB Agar................................................197LICNR Broth .......................................................197

Listeria Enrichment Broth ...................................197Listeria Enrichment Broth, FDA .........................197Listeria Enrichment Broth I, USDA FSIS ...........198Listeria Enrichment Broth II, USDA FSIS..........198Listeria Fermentation Broth.................................199Listeria Transport Enrichment Medium ..............199Litmus Lactose Agar............................................199Litmus Lactose Agar with Crystal Violet ............200Litmus Milk .........................................................200Liver Broth...........................................................200Liver Broth with NaCl .........................................201Liver Infusion Sake Medium ...............................201Liver Veal Agar....................................................201Liver Veal Egg Yolk Agar....................................201LMX Broth Modified, Fluorocult ........................202Long-Term Preservation Medium........................202Lowenstein-Gruft Medium ..................................202Lowenstein-Jensen Medium ................................203Lowenstein-Jensen Medium with NaCl...............203Lowenstein-Jensen Medium

with Streptomycin .........................................203Lowenstein-Jensen Medium

without Glycerol............................................204LPM Agar ............................................................204LPM Agar with

Esculin and Ferric Iron ..................................205LS Differential Medium.......................................205Lysine Agar, Selective .........................................205Lysine Arginine Iron Agar ...................................206Lysine Decarboxylase Broth, Falkow..................206Lysine Broth Falkow with NaCl ..........................206Lysine Decarboxylase Broth,

Taylor Modification.......................................206Lysine Decarboxylase Broth,

Taylor Modification.......................................206Lysine Decarboxylase Medium ...........................207Lysine Iron Agar, Edward and Fife......................207Lysine Medium ....................................................207Lysine Ornithine Mannitol Agar..........................208Lysozyme Broth...................................................208M16 Agar.............................................................208M17 Agar.............................................................209M17 Agar.............................................................209M56 Agar.............................................................209M Broth................................................................209M10 Broth............................................................210M17 Broth............................................................210M9 Medium .........................................................211M56 Medium .......................................................211M17 Medium for Lactic Streptococci..................211M17 Medium, Modified ......................................212M9 Medium with Arginine ..................................212MACA with Maltose ...........................................213MacConkey Agar.................................................213MacConkey Agar, CS ..........................................213MacConkey Agar, Fluorocult ..............................213

MacConkey Agar No. 2.......................................214MacConkey Agar No. 3.......................................214MacConkey Agar without Crystal Violet ............214MacConkey Agar without Salt ............................214MacConkey Broth................................................215MacConkey Broth, Purple ...................................215Magnesium Oxalate Agar ....................................215Malachite Green Broth ........................................215Malonate Broth ....................................................215Malonate Broth, Ewing Modified........................216Malt Agar.............................................................216Malt and Peptone Medium...................................216Malt Extract Agar ................................................217Malt Extract Agar, Half Strength.........................217Malt Extract Agar for Yeasts and Molds .............217Malt Extract Broth ...............................................217Malt Extract Broth ...............................................217Malt Extract Broth ...............................................218Malt Extract Yeast Extract

40% Glucose Agar ........................................218Malt Yeast Extract 50% Glucose Agar ................218Manganese Nutrient Agar....................................218Mannitol Agar......................................................218Mannitol Egg Yolk Polymyxin Agar ...................218Mannitol Egg Yolk Polymyxin Agar ...................219Mannitol Maltose Agar........................................219Mannitol Salt Agar ..............................................220Mannitol Salt Broth .............................................220Mannitol Yolk Polymyxin Agar ..........................220Maximum Recovery Diluent ...............................221McBride Agar, Modified .....................................221McBride Listeria Agar.........................................221McClung Carbon-Free Broth...............................221McClung-Toabe Agar ..........................................221McClung-Toabe Agar, Modified .........................222McClung-Toabe Agar, Modified .........................223McClung-Toabe Egg Yolk Agar,

CDC Modified...............................................223m-CP Medium .....................................................224MD Medium ........................................................224Medium for Aciduric,

Thermophilic Bacillus Strains .......................225Medium for Halophilic Bacilli.............................225Medium for Lactobacilli ......................................225Medium N............................................................225Medium S.............................................................226Membrane Clostridium perfringens Medium......226Membrane Lactose Glucuronide Agar.................227Membrane Lauryl Sulfate Broth..........................227Methylene Blue Milk Medium ............................227MIB with Maltose................................................227Micro Assay Culture Agar...................................227Micro Inoculum Broth .........................................228Microbial Content Test Agar ...............................228Micrococcus Medium ..........................................228Micrococcus Medium, FDA ................................228

Middlebrook 13A Medium ..................................228Middlebrook ADC Enrichment ...........................229Middlebrook 7H9 Broth with

Middlebrook ADC Enrichment .....................229Middlebrook 7H9 Broth with

Middlebrook OADC Enrichment ..................229Middlebrook 7H9 Broth with

Middlebrook OADC Enrichment and Triton WR 1339......................................230

Middlebrook 7H10 Agar with Middlebrook ADC Enrichment .....................230

Middlebrook 7H10 Agar with Middlebrook OADC Enrichment ..................231

Middlebrook 7H10 Agar with Middlebrook OADC Enrichment and Hemin .....................231

Middlebrook 7H10 Agar with Middlebrook OADC Enrichment and Triton WR 1339......................................232

Middlebrook OADC Enrichment.........................232Middlebrook OADC Enrichment

with Triton WR 1339.....................................232MIL Medium........................................................233Milk Agar.............................................................233Minerals Modified Glutamate Agar.....................233Minerals Modified Medium.................................233Minimal Agar I ....................................................234Minimal Agar II ...................................................234Minimal Agar III..................................................234Minimal Broth I ...................................................235Minimal Broth II ..................................................235Minimal Broth III.................................................236MLCB Agar .........................................................236Modified Campylobacter Blood-Free

Selective Agar Base.......................................236Modified Oxford Listeria Selective Agar............237Motility Nitrate Medium......................................237Modified Semisolid Rappaport

Vassiliadis Medium .......................................238Mller Decarboxylase Broth................................238Mller KCN Broth Base ......................................238Motility Indole Ornithine Medium ......................238Motility Indole Ornithine Medium ......................239Motility Medium..................................................239Motility Medium S...............................................239Motility Nitrate Agar ...........................................239Motility Nitrate Medium......................................240Motility Nitrate Medium, Buffered......................240Motility Sulfide Medium .....................................240Motility Test and Maintenance Medium..............240Motility Test and Maintenance Medium..............241Motility Test and

Maintenance Medium, Gilardi ......................241Motility Test and

Maintenance Medium, Tatum........................241Motility Test Medium ..........................................241

Motility Test Medium, Semisolid with NaCl ......................................................241

Motility Test Medium, Semisolid ........................242MP 5 Medium......................................................242MP 7 Medium......................................................243MPH Agar............................................................243MRS Agar............................................................243MRS Agar with Cysteine.....................................244MRS Agar with Ethanol ......................................244MRS Agar with Tomato Juice pH 5.2 .................244MRS Agar, Half Strength ....................................244MRS Broth...........................................................245MRS Broth with CaCO3 ......................................245MRS Broth with Cysteine....................................245MRS Broth with Ethanol .....................................246MRS Chalk ..........................................................246MRS Medium ......................................................246MRS with Fermented Wort..................................247MRVP Broth ........................................................247MRVP Medium....................................................247MRVP Broth with NaCl.......................................248Mucate Broth .......................................................248Mucate Control Broth ..........................................248Mueller-Hinton Agar ...........................................249Mueller-Hinton Agar with Horse Blood..............249Mueller-Hinton Agar with NaCl..........................249MY10-12 Medium...............................................249MY20 Agar..........................................................249MY40 Agar..........................................................249MY60 Agar..........................................................250Mycobactosel Agar..............................................250Mycological Broth ...............................................250Mycological Broth with Low pH.........................250Mycophil Agar.....................................................251Mycophil Agar with Low pH ..............................251Mycophil Broth....................................................251Mycosel Agar.......................................................251Neopeptone Glucose Agar...................................251Neopeptone Glucose Rose Bengal

Aureomycin Agar ..........................................251Niacin Assay Medium .........................................252Nickels and Leesment Agar, Modified ................252Nitrate Agar .........................................................253Nitrate Broth ........................................................253Nitrate Broth, Campylobacter .............................253Nitrate Broth, Enriched........................................253Nitrate Broth, Enriched........................................254Nitrate Reduction Broth.......................................254Nitrate Reduction Broth, Clark............................254Nitrate Reduction Broth for

Pseudomonas and Related Genera ................254Nitrate Reduction Medium and Reagents............255Nonfat Dry Milk, Reconstituted ..........................255Novobiocin Agar .................................................255Nutrient Agar .......................................................256

Nutrient Agar, 1.5%.............................................256Nutrient Agar No.2 ..............................................256Nutrient Agar, pH 6.0 ..........................................257Nutrient Agar with 0.5% NaCl ............................257Nutrient Agar

with 0.5% NaCl, pH 9.5-10.0 ........................257Nutrient Agar with 0.5% NaCl

and Sodium Citrate ........................................257Nutrient Agar with 3% NaCl ...............................257Nutrient Agar with Phytone.................................258Nutrient Agar with Potato Starch.........................258Nutrient Agar with 2% Sucrose ...........................258Nutrient Broth ......................................................258Nutrient Broth No. 2 ............................................258Nutrient Broth Salts Medium...............................258Nutrient Broth, Standard II ..................................259Nutrient Gelatin ...................................................259Nutrient Gelatin, CDC .........................................259NZC Broth ...........................................................259NZCYM Medium ................................................259NZY Agar ............................................................260NZY Broth ...........................................................260O157:H7 ID Agar ................................................260Oatmeal Agar A ...................................................260Oatmeal Agar .......................................................260Oatmeal Agar .......................................................261Oatmeal Salts Agar ..............................................261Ogawa TB Medium .............................................261Oleic Albumin Complex......................................261ONPG Broth ........................................................261OR Indicator Agar ...............................................262Orange Serum Agar .............................................262Orange Serum Broth Concentrate 10X................262Organic Acid Medium KP ...................................263Ornithine Broth ....................................................263Ornithine Broth with NaCl ..................................263Oxford Agar .........................................................263Oxford Agar, Modified ........................................264Oxford Medium ...................................................264Oxidative-Fermentative

Glucose Medium, Semisolid .........................264Oxidative-Fermentative

Glucose Medium, Semisolid with NaCl........265Oxidation-Fermentation Medium ........................265Oxidation-Fermentation

Medium, Hugh-Leifsons ..............................265Oxidation-Fermentation Medium, Kings............265Oxidative-Fermentative

Glucose Medium, Semisolid, with NaCl.......266Oxidative-Fermentative Medium.........................266Oxidative-Fermentative Test Medium .................266Oxidative-Fermentative Test Medium

with NaCl ......................................................267Oxytetracycline Glucose

Yeast Extract Agar.........................................267

OZR Medium.......................................................267P Agar ..................................................................268PA-C Agar............................................................268Pablum Cereal Agar.............................................268Pai Medium..........................................................268PALCAM Agar ....................................................268Papain ..................................................................269Park and Sanders Enrichment Broth....................269PE2 Medium ........................................................269Pectin Medium.....................................................270Penicillin-Streptomycin Solution.........................270Peptone Iron Agar................................................270Peptone Medium..................................................270Peptone Sorbitol Bile Broth.................................270Peptone Water ......................................................271Peptone Water with Andrades Indicator .............271Peptone Yeast Trypticase Agar ............................271Pfizer Selective Enterococcus Agar.....................271PGA .....................................................................272PGLE Medium.....................................................272Phenethyl Alcohol Agar ......................................272Phenol Red Agar..................................................272Phenol Red Broth.................................................273Phenol Red Carbohydrate Broth..........................273Phenol Red Carbohydrate Broth

with NaCl ......................................................273Phenol Red Glucose Broth...................................274Phenol Red Glucose Broth...................................274Phenol Red Glucose Broth with NaCl .................274Phenol Red Lactose Agar ....................................275Phenol Red Lactose Broth ...................................275Phenol Red Mannitol Agar ..................................275Phenol Red Mannitol Broth .................................275Phenol Red Sucrose Broth ...................................275Phenol Red Tartrate Agar ....................................276Phenol Red Tartrate Broth ...................................276Phenylalanine Agar..............................................276Phenylalanine Malonate Broth ............................277Phenylethanol Agar .............................................277Phenylethanol Blood Agar...................................277Pisu Medium........................................................277PL Agar................................................................278Plate Count Agar..................................................278Plate Count Agar, Modified.................................278Plate Count Agar, Special ....................................278Plate Count Agar with Antibiotic ........................278Plate Count Agar with

Antibiotic-Free Skim Milk ............................279Plate Count Broth ................................................279PM Indicator Agar ...............................................279PMP Broth ...........................................................279Poly--Hydroxybutyrate Medium .......................279Polymyxin Pyruvate Egg Yolk

Mannitol Bromthymol Blue Agar .................280Polymyxin Staphylococcus Medium ...................280

Polypectate Gel Medium .....................................280Polysorbate 80 Agar.............................................280Pork Plasma Fibrinogen Overlay Agar................281Potassium Cyanide Broth.....................................281Potassium Tellurite Agar .....................................282Potato Agar with Cereal Culms ...........................282Potato Dextrose Adenine Agar ............................282Potato Dextrose Agar...........................................283Potato Dextrose Agar, pH 5.0 ..............................284Potato Dextrose Agar, 1/3 Strength .....................284Potato Dextrose Agar, Quarter Strength ..............284Potato Dextrose Agar and Yeast Medium............284Potato Dextrose Agar with Antibiotics................285Potato Dextrose Agar with Gentamycin ..............285Potato Dextrose Agar with

2% Glucose and 60% Sucrose.......................285Potato Dextrose Agar with Thiamine ..................285Potato Dextrose Broth..........................................285Potato Dextrose Broth

with Yeast Extract..........................................286Potato Dextrose L-Isoleucine Agar ......................286Potato Dextrose Salt Agar....................................286Potato Dextrose Yeast Agar .................................286Potato Extract Agar..............................................287Potato Flakes Agar...............................................287Potato Malt Agar..................................................287Powell and Erringtons Medium..........................287Preenrichment Medium........................................288Preston Enrichment Broth....................................288Presumpto Media .................................................288Pril Xylose Ampicillin Agar ................................289Propionibacterium Agar ......................................289Propionibacterium Medium.................................289Proskauer-Beck Medium

for Mycobacterium ........................................289Proteose Agar.......................................................290Proteose No. 3 Agar.............................................290Proteose Yeast Extract Medium...........................290Pseudomonas aeruginosa Agar ...........................290Pseudomonas Agar F ...........................................290Pseudomonas Agar F ...........................................291Pseudomonas Agar P ...........................................291Pseudomonas Basal Mineral Medium .................291Pseudomonas CFC Agar......................................291Pseudomonas CN Agar........................................292Pseudomonas Isolation Agar ...............................292Pseudomonas Medium.........................................292Pseudomonas Medium.........................................293Pseudomonas Medium A.....................................293Pseudomonas Medium B.....................................293Pseudomonas saccharophila Medium.................293Purple Agar ..........................................................294Purple Broth.........................................................294Purple Broth with NaCl .......................................295

Purple Carbohydrate Fermentation Broth Base .....................................................295

Purple Carbohydrate Fermentation Broth Base with Esculin................................295

Purple Lactose Agar ............................................296Purple Serum Agar Base......................................296PY Medium with Glucose ...................................296PYG Broth ...........................................................297Pyrazinamidase Agar ...........................................297Pyruvate Utilization Medium ..............................297R2 Broth...............................................................297R2A Agar.............................................................298R3A Agar.............................................................298Rainbow Agar O157 ............................................298Rainbow Agar Salmonella ...................................298Raka-Ray Agar ....................................................299Rambach Agar .....................................................299RAPIDE. coli 2 Agar..........................................300RAPIDEnterococcus Agar .................................300RAPIDL. mono Medium ....................................300Rappaport Broth, Modified..................................300Rappaport-Vassiliadis

Enrichment Broth ..........................................301Rappaport-Vassiliadis R10 Broth ........................301Rappaport-Vassiliadis

Soya Peptone Broth.......................................301RB-1/RB-9 Medium ............................................301Reinforced Clostridial Medium ...........................302Reinforced Clostridial Medium

with Sodium Lactate......................................302Riboflavin Assay Medium...................................302Riboflavin Medium..............................................303Rimler-Shotts Medium ........................................303Rippey-Cabelli Agar............................................303Rogosa Agar ........................................................303Rogosa Broth, Modified ......................................304Rogosa SL Agar...................................................304Rogosa SL Broth..................................................304Rose Bengal Chloramphenicol Agar ...................305Russell Double-Sugar Agar .................................305S. aureus ID .........................................................305S Broth .................................................................305SA Agar ...............................................................305SA Agar, Modified ..............................................305Sabouraud Agar ...................................................306Sabouraud Agar,

Diluted 1/10...................................................306Sabouraud Agar, Modified ..................................306Sabouraud Dextrose Agar....................................306Sabouraud Dextrose Broth...................................306Sabouraud Maltose Agar .....................................306Sabouraud Maltose Broth ....................................307Sabouraud Medium, Fluid ...................................307Saccharolytic Clostridia Medium ........................307Saccharomyces Medium ......................................307

Saccharomyces rouxii Medium............................308Salmonella Chromogen Agar...............................308Salmonella Chromogenic Agar Base...................308Salmonella Medium.............................................308Salmonella Rapid Test Elective Medium.............308Salmonella Rapid Test

Elective Medium, 2X ....................................309Salmonella Shigella Agar ....................................309Salmonella Shigella Agar, Modified....................309Salt Broth, Modified ............................................309Salt Colistin Broth ...............................................310Salt Meat Broth....................................................310Salt Polymyxin Broth...........................................310Salt Tolerance Medium........................................310Salt Tolerance Medium........................................311Salt Tolerance Medium, Gilardi...........................311Salt Tolerance Medium, Tatum............................311Sanfrancisco Medium ..........................................311SBG Enrichment Broth........................................311SBG Sulfa Enrichment.........................................312SC Agar................................................................312SC Broth ..............................................................312Schaedler Agar.....................................................313Schaedler Agar.....................................................313Schleifer-Krmer Agar.........................................313Seawater Agar......................................................314Seawater Agar......................................................314Seawater Agar with Fetal Calf Serum .................314Seawater Agar with Horse Blood ........................315Seawater Yeast Extract Broth, Modified..............315Selection Agar I ...................................................315Selection Agar II ..................................................315Selenite Broth ......................................................315Selenite Broth Base, Mannitol .............................316Selenite Cystine Broth .........................................316Selenite F Broth ...................................................316Semisolid Brucella Broth.....................................316Semi-Solid Medium, Modified ............................317Semisolid Medium, Modified

with Glycine and Neutral Red .......................317Semisolid Medium, Modified

with NaCl and Neutral Red ...........................317Semisolid Medium, Modified

with Cysteine and Neutral Red......................317Semisolid Medium, Modified

with Nitrate and without Neutral Red ...........318Semisolid Medium for Motility ...........................318Serum Glucose Agar ............................................318Serum Glucose Agar, Farrell Modified ...............319Serum Potato Infusion Agar ................................319Seven H11 Agar ...................................................320Seven-Hour Fecal Coliform Agar........................320SF Broth...............................................................320SFP Agar..............................................................321Sheep Blood Agar................................................321

Sheep Blood Agar................................................322Shigella Broth ......................................................322SI Agar.................................................................322Sierra Medium .....................................................322SIM Medium........................................................323SIM Motility Medium..........................................323Simmons Citrate Agar ........................................323Single-Layer Agar ...............................................323Single-Layer Agar ...............................................324Skim Milk Agar ...................................................324Skirrow Brucella Medium ...................................324SL Medium ..........................................................325Slad Medium........................................................325Slanetz and Bartley Medium ...............................325SM ID2 Agar .......................................................326Sodium Chloride Broth, 6.5.................................326Sodium Dodecyl Sulfate

Polymyxin Sucrose Agar...............................326Sodium Lactate Agar ...........................................326Sodium Lactate Agar, Modified ..........................326Soft Agar Gelatin Overlay ...................................326Sorbitol Agar .......................................................327Sorbitol MacConkey Agar...................................327Sorbitol MacConkey Agar...................................327Sorbitol MacConkey Agar with BCIG ................328Sour Dough Medium ...........................................328Spirit Blue Agar...................................................328Spore Strip Broth .................................................328Sporulation Broth.................................................329Sprays Fermentation Medium ............................329SPS Agar..............................................................329SS Deoxycholate Agar.........................................329SSM Agar ............................................................330ST Holding Medium............................................330STAA Agar Base .................................................330Standard Methods Agar .......................................330Standard Methods Agar

with Lecithin and Polysorbate 80..................331Standard Methods Broth ......................................331Standard Methods Caseinate Agar.......................331Standard II Nutrient Agar ....................................331Staphylococcus Agar No. 110..............................331Staphylococcus Broth ..........................................332Staphylococcus Medium ......................................332Staphylococcus Medium No. 110 ........................332Staphylococcus/Streptococcus

Selective Medium..........................................332Starch Agar ..........................................................333Starch Hydrolysis Agar........................................333Sterility Test Broth...............................................333Stock Culture Agar ..............................................334Stock Culture Agar with L-Asparagine................334Stonebrinks Medium...........................................334Strep ID Quad Plate .............................................335Streptococcal Growth Medium............................335

Streptococcus Agar ..............................................335Streptococcus Medium.........................................335Streptococcus Selective Medium .........................335Streptococcus thermophilus Agar ........................336Succinate Mineral Medium..................................336Sucrose Agar........................................................336Sucrose Teepol Tellurite Agar .............................337Sucrose Yeast Extract Medium............................337Superbroth............................................................337Syncase Broth ......................................................337T1N0 Broth...........................................................337T1N1 Agar ............................................................338T1N2 Agar ............................................................338T1N1 Broth...........................................................338T1N3 Broth...........................................................338T1N6 Broth...........................................................338T1N8 Broth...........................................................338T1N10 Broth .........................................................339T7 Agar Base .......................................................339Tap Water Agar ....................................................339Tarshis Blood Agar ..............................................339TB Nitrate Reduction Broth.................................339TBX Agar ............................................................339TBYA Agar ..........................................................340TCBS Agar ..........................................................340TCH Medium.......................................................340Teepol Broth, Enriched ........................................341Tellurite Glycine Agar .........................................341Tergitol 7 Agar .....................................................342Tergitol 7 Broth....................................................342Tetrathionate Broth ..............................................343Tetrathionate Broth ..............................................344Tetrathionate Broth, Hajna...................................344Tetrathionate Broth, USA ....................................344Tetrathionate Broth with Novobiocin ..................345Tetrathionate Crystal Violet

Enhancement Broth .......................................345Tetrazolium Thallium Glucose Agar ...................345Tetrazolium Tolerance Agar ................................345TGE Broth............................................................346Thermoacidurans Agar ........................................346Thermophilic Bacillus Medium...........................346Thiamine Assay Medium.....................................346Thiamine Assay Medium LV...............................347Thiogel Medium ..................................................347Thioglycolate Broth USP, Alternative .................347Thioglycolate Gelatin Medium............................347Thioglycolate Medium.........................................347Thioglycolate Medium, Brewer...........................348Thioglycolate Medium, Brewer Modified ...........348Thioglycolate Medium, Enriched ........................348Thioglycolate Medium, Fluid ..............................349Thioglycolate Medium, USP ...............................349Thioglycolate Medium with 20% Bile.................349Thioglycolate Medium without Glucose .............350

Thioglycolate Medium without Glucose and Indicator ......................350

Thioglycolate Medium without Indicator............350Thioglycolate Medium without Indicator............350TNT Medium.......................................................351Todd-Hewitt Broth...............................................351Todd-Hewitt Broth, Modified..............................351Toluidine Blue DNA Agar...................................351Toluidine Blue DNA Agar...................................352Tomato Juice Agar ...............................................352Tomato Juice Agar Special ..................................352Tomato Juice Broth..............................................352Tomato Juice Broth..............................................353Tomato Juice Medium .........................................353Tomato Juice Milk Agar ......................................353Tomato Juice Yeast Extract Medium ...................353Tomato Juice Yeast Extract Milk Medium ..........353TPEY Agar ..........................................................354TPGY Medium ....................................................354Tributyrin Agar ....................................................354Trimethylamine N-Oxide Medium ......................354Triple Sugar Iron Agar.........................................355Trypaflavin Nalidixic Acid Serum Agar .............356Tryptic Nitrate Medium.......................................356Tryptic Soy Agar

with Magnesium Sulfate................................356Tryptic Soy Agar with Magnesium

Sulfate and Sodium Chloride ........................356Tryptic Soy Agar with Sodium Chloride.............357Tryptic Soy Agar with 0.6% Yeast Extract..........357Tryptic Soy Fast Green Agar ...............................357Trypticase Agar Base...........................................357Trypticase Agar Base with Carbohydrate............357Trypticase Glucose Extract Agar .........................357Trypticase Novobiocin Broth...............................358Trypticase Peptone Glucose

Yeast Extract Broth........................................358Trypticase Peptone Glucose

Yeast Extract Broth, Buffered .......................358Trypticase Peptone Glucose

Yeast Extract Broth with Trypsin ..................358Trypticase Soy Agar ............................................359Trypticase Soy Agar

with Lecithin and Polysorbate 80..................359Trypticase Soy Agar-Magnesium

Sulfate-NaCl Agar.........................................359Trypticase Soy Agar with 3% NaCl ....................360Trypticase Soy Agar with NaCl...........................360Trypticase Soy Agar with NaCl,

Horse Serum, and Penicillin..........................360Trypticase Soy Agar Yeast Extract ......................360Trypticase Soy Broth ...........................................360Trypticase Soy Broth, Modified ..........................361Trypticase Soy Broth with 10 mM Glucose.........361Trypticase Soy Broth with Ferrous Sulfate..........361

Trypticase Soy Broth with Glycerol ....................361Trypticase Soy Broth with Sodium

Chloride and Sodium Pyruvate......................362Trypticase Soy Broth

with Yeast Extract..........................................362Trypticase Soy Broth without Glucose ................362Trypticase Soy Broth Yeast Extract .....................362Trypticase Soy Polymyxin Broth.........................362Trypticase Soy-Sheep Blood Agar.......................363Trypticase Soy Tryptose Broth ............................363Trypticase Soy Yeast Extract Agar ......................363Trypticase Yeast Extract Glucose Medium..........364Tryptone Agar ......................................................364Tryptone B