ATLAS DE HEMATOLOGIA CLINICASEXTA EDICION 2004

H. Loffler _ J. Rastetter _ T. HaferlachMethodology

I Techniques of Specimen Collection and Preparation 3Blood Smear 4Bone Marrow 4Fine-Needle Aspiration of Lymph Nodes and Tumors 5Splenic Aspiration 6Concentrating Leukocytes from Peripheral Blood in Leukocytopenia 6Demonstration of Sickle Cells 6II Light Microscopic Procedures 71 Staining Methods for the Morphologic and CytochemicalDifferentiation of Cells 81.1 Pappenheim’s Stain (Panoptic Stain) 81.2 Undritz Toluidine Blue Stain for Basophils 81.3 Mayer’s Acid Hemalum Nuclear Stain 81.4 Heilmeyer’s Reticulocyte Stain 81.5 Heinz Body Test of Beutler 81.6 Nile Blue Sulfate Stain 91.7 Kleihauer-Betke Stain for Demonstrating Fetal Hemoglobinin Red Blood Cells 91.8 Kleihauer-Betke Stain for Demonstrating Methemoglobin-Containing Cells in Blood Smears 101.9 Berlin Blue Iron Stain 101.10 Cytochemical Determination of Glycogen in Blood Cellsby the Periodic Acid Schiff Reaction and Diastase Test(PAS Reaction) 111.11 Sudan Black B Stain 131.12 Cytochemical Determination of Peroxidase 131.13 Hydrolases 131.14 Appendix 16XI

Immunocytochemical Detection of Cell-Surface and IntracellularAntigens 183 Staining Methods for the Detection of Blood Parasites 193.1 “Thick Smear” Method 193.2 Bartonellosis 193.3 Detection of Blood Parasites in Bone Marrow Smears 193.4 Toxoplasmosis 193.5 Microfiliariasis 193.6 Mycobacterium Species (M. tuberculosis, M. leprae) 19IllustrationsIII Overview of Cells in the Blood, Bone Marrow,and Lymph Nodes 23IV Blood and Bone Marrow 274 Individual Cells 28

4.1 Light Microscopic Morphology and Cytochemistry 285 Bone Marrow 675.1 Composition of Normal Bone Marrow 695.2 Disturbances of Erythropoiesis 805.3 Reactive Blood and Bone Marrow Changes 1075.4 Bone Marrow Aplasias (Panmyelopathies) 1185.5 Storage Diseases 1225.6 Hemophagocytic Syndromes 1295.7 Histiocytosis X 1325.8 Chronic Myeloproliferative Disorders (CMPD) 1345.9 Myelodysplastic Syndromes (MDS) 1585.10 Acute Leukemias 1705.11 Neoplasias of Tissue Mast Cells (Malignant Mastocytoses) 286V Lymph Nodes and Spleen 2936. Cytology of Lymph Node and Splenic Aspirates 2946.1 Reactive Lymph Node Hyperplasia 2956.2 Infectious Mononucleosis 3046.3 Persistent Polyclonal B Lymphocytosis 3076.4 Malignant Non-Hodgkin LymphomasVI Tumor Aspirates from Bone Marrow Involvedby Metastatic Disease 385VII Blood Parasites and Other Principal Causative Organismsof Tropical Diseases 3997 Blood Parasites 4007.1 Malaria 4007.2 African Trypanosomiasis (Sleeping Sickness) 4107.3 American Trypanosomiasis (Chagas Disease) 4117.4 Kala Azar or Visceral Leishmaniasis 4147.5 Cutaneous Leishmaniasis (Oriental Sore) 4167.6 Toxoplasmosis 4167.7 Loa Loa 4177.8 Wuchereria bancrofti and Brugia malayi 4177.9 Mansonella (Dipetalonema) Perstans 4208 Further Important Causative Organismsof Tropical Diseases 4218.1 Relapsing Fever 4218.2 Bartonellosis (Oroya Fever) 4218.3 Leprosy 423Subject Index 425