[ ]13 [ ]14

Atlantis HPLC Columns

T he Atlantis family of HPLC columns was created to solve one of the most challenging chromatographic

problems, retaining polar compounds. Often difficult to retain by traditional reversed-phase (RP) chromatography,

polar analytes typically elute in the void volume of the column or co-elute with endogenous species present within

biological matrices, adversely affecting the precision and accuracy of quantitative bioanalytical assays. T hrough

historical experience and synthetic innovation, Atlantis columns lead the industry by providing exceptional

performance, versatility, retention and chromatographic stability for polar compounds, while also affording

balanced retention for broad analyte mixtures.

[ ]3

Atlantis T3 Columns: Versatility by Innovation

Reversed-phase chromatography is the most widely used technique in LC separations due to its versatility to retain and resolve a number

of different types of compounds. The culmination of more than three decades of experience and understanding in stationary-phase design

has resulted in the creation of the Atlantis T3 columns. Providing balanced retention of polar and hydrophobic molecules, Atlantis T3

columns solve one of the largest analytical problems afflicting chromatographers. Furthermore, selective optimization of pore diameter,

C18 -ligand density and endcapping, enable Atlantis T3 columns to provide paramount retention for polar compounds, while exhibiting

industry leading peak shape and efficiency for all analytes as well as exceptional chemical stability across a wide pH range.

The brilliant performance provided by T3 bonding technology can be achieved across HPLC and UPLC Technology platforms. ACQUITY

UPLC HSS T3 columns incorporate the same T3 bonding and endcapping technology as Atlantis T3 columns, thus providing the same

capability independent of your separation platform.

Atlantis HILIC Silica Columns: Enhanced Retention of Polar Compounds

Atlantis HILIC Silica columns improve retention of very polar analytes and metabolites that are poorly retained by reversed-phase

chromatography by Hydrophilic Interaction Chromatography (HILIC). In addition to enhanced retention of polar compounds, Atlantis HILIC

Silica columns provide increased mass spectrometry response, direct compatibility with SPE eluates as well as orthogonal selectivity

compared to reversed-phase materials making HILIC an attractive alternative separation technique.

[ ]4

Redefining PolaR ComPound Ret ent ion

T he exceptional performance of Atlantis T3 HPLC columns is a result of intelligent stationary phase design. Waters advanced T3 bonding process utilizes a trifunctional C18-alkyl phase, bonded at an intermediate 1.6 mol/m2

ligand density to promote polar compound retention*1. T his lower ligand density allows analytes to more readily access the pore structure of the material, where greater than

99% of chromatographic retention occurs, resulting in enhanced retention.

*1 P.D. McDonald et al. "Topics in Liquid Chromatography Part 1. Designing a Reversed-Phase Column for Polar Compound Retention" Waters W hitepaper 2006, 8 pp; search for

720001889EN on waters.com

ConditionsColumn: Atlantis T3, 4.6 x 150 mm, 5 mPart Number: 186003747Mobile Phase A: H2OMobile Phase B: ACNMobile Phase C: 100 mM HCOONH4, pH 3.0Flow Rate: 1.0 mL/minGradient: Time Profile (min) %A %B %C 0.00 95 0 5 5.00 95 0 5 15.00 70 25 5 20.00 70 25 5 21.00 95 0 5 25.00 95 0 5Injection Volume: 10 LColumn Temperature: 30 CDetection: UV @ 280 nm

Compounds: 1. Norepinephrine (NE) 2. Epinephrine (E) 3. 3,4-Dihydroxyphenylalanine (DOPA) 4. Dopamine (DA) 5. 4-Hydroxy-3-methoxymandelic acid (VMA) 6. Serotonin(5-HT) 7. 3-Methoxy-4-hydroxyphenylglycol (MHPG) 8. 3,4-Dihydroxyphenylacetic acid (DOPAC) 9. 5-Hydroxy-3-indoleacetic acid (5-HIAA)10. 4-Hydroxy-3-methoxyphenylacetic acid (HVA)

1

2

3

4

75

6

8

9

10

6 10 12 14 18 20 min0 2 4 8 16

ATLANTIS T3

[ ACQUITY UPLC SYSTem ACCeSSorIeS ]

[ ]5

BalanC ed Ret ent ion of div eRse analyt e mixtuRes

When faced with the task of analyzing a number of compounds encompassing a broad range of polarity, few options exist that will result in adequate retention of polar species without excessive retention of the less polar components. In drug discovery, a large number of candidates need to be screened, often with little or no previous characterization of their chemical properties. Many polar candidates are often overlooked due to their elution in the void space of the column, while hydrophobic species may be permanently bound to the stationary phase.

Atlantis T3 columns are designed to deliver exceptional performance for an expansive range of compound polarities, thus being ideally suited as a universal reversed-phase column for open access environments.

ConditionsColumn: Atlantis T3, 4.6 x150 mm, 5 mPart Number: 186003747Mobile Phase A: H2OMobile Phase B: ACNMobile Phase C: 1% HCOOH, pH 2.3Mobile Phase D: MeOHFlow Rate: 2.0 mL/minGradient: Time Profile (min) %A %B %C %D 0.00 55 17.5 10 17.5 4.00 35 27.5 10 27.5 6.00 9 40.0 10 41.0 15.00 9 40.0 10 41.0 16.00 55 17.5 10 17.5 20.00 55 17.5 10 17.5Injection Volume: 10 L Column Temperature: 30 CDetection: UV @ 280 nm

Compounds:1. Propyl gallate2. 2,4,5-Trihydroxybutyrophenone3. Tert-butylhydroquinone4. Butylated hydroxyanisole5. 2,6-Di-tert-butyl-4-hydroxymethylphenol6. Octyl gallate7. Butylated hydroxytoluene8. Lauryl gallate

0 2 4 6 8 10 12 14 min

1 2

3

4

5

6

7

8

Hydrophobic: lipid soluble antioxidants

ConditionsColumn: Atlantis T3, 4.6 x150 mm, 5 mPart Number: 186003747Mobile Phase A: H2OMobile Phase B: ACNMobile Phase C: 100 mM CH3COONH4, pH 5.0Flow Rate: 1.0 mL/minGradient: Time Profile (min) %A %B %C 0.00 90 0 10 10.00 84 6 10 11.00 90 0 10 15.00 90 0 10Injection Volume: 10 LColumn Temperature: 30 CDetection: UV @ 254 nm

Compounds:1. Cytosine2. 5-Fluorocytosine3. Uracil4. 5-Fluorouracil5. Guanine6. Thymine7. Adenine

0 1 2 3 4 5 6 7 8 9 10 min

1

2

3

6

7

54

Polar: nucleic acid Bases

Retention of Uracil

AtlAntis t3

AtlAntis t3

5

12

34

5

1 342

1 2

3,4

5

1

2,3,4

5

0 1 2 3 4 5 6 7 8 9 min

0 1 2 3 4 5 6 7 8 9 min

Waters stop flow test determines the susceptibility of a stationary phase to pore dewetting using 100% aqueous mobile phases. Under these difficult test conditions, Atlantis T3 columns exhibit negligible retention loss where as the high coverage C18 column suffers complete retention loss.

ConditionsColumns: 4.6 x 100 mm, 3 mMobile Phase: 10 mM HCOONH3, pH 3.0Flow Rate: 1.2 mL/minInjection Volume: 7.0 LDetection: UV @ 254 nm

Compounds:1. Thiourea2. 5-Fluorocytosine3. Adenine4. Guanosine-5-monophosphate5. Thymine

atlantis t3, intermediate Coverage C18

Zorbax eclipse Plus C18, High Coverage C18

100% Retention Lost

7% Retention Lost

Atlan

tis T3

[ ]6

Ret ent ion wit Hout ion-PaiRing Reagents

The use of ion-pairing reagents on reversed-phase columns is a common approach to retain highly polar, ionizable compounds. As mass spectrometry has become a prominent detection technique in many laboratories, ion-pairing reagents become a less attractive option due to the suppression they cause to the MS response. Atlantis T3 columns exhibit enhanced retention without the need for such reagents, enabling lower limits of detection and quantitation for LC/MS methods.

ConditionsColumns: Atlantis T3, 2.1 x 50 mm, 3 mPart Number: 186003717Mobile Phase A: 0.1% HCOOHMobile Phase B: ACNFlow Rate: 0.5 mL/minGradient: Time Profile (min) %A %B 0.00 98 2 5.00 2 98Injection Volume: 15 LColumn Temp.: 45 CMRM: morphine 286 > 201 morphine-3-D-glucuronide 462 > 286 morphine-6-D-glucuronide 462 > 286 morphine N-oxide 302 > 162 6-acetylmorphine 328 > 165 10-hydroxymorphine 302 > 58

Compounds:1. 10-Hydroxymorphine2. Morphine-3-D-glucuronide3. Morphine-6-D-glucuronide4. Morphine5. Morphine N-oxide6. 6-Acetylmorphine

6

1

2

3

4

5

1.0 1.5 min.50

HO

O

HO

NCH3

HOH

HO

O

O

NCH3

H

O

OHOH

OHOHO

O

O

HO

NCH3

H

O

HO HO

OHOHO

HO

O

HO

NCH3

H

HO

O

O

NCH3

H

O

HO

O

HO

NH +

O

10-Hydroxymorphine

Morphine-3-D-glucuronide Morphine-6-D-glucuronide

Morphine

Morphine N-oxide

6-Acetylmorphine

oPt imiZed ResistanC e to dew et t ing in 100% aqueous moBile PHases

In reversed-phase chromatography, an effort to increase the affinity of a polar molecule to the stationary phase is attempted by reducing the organic content in the mobile phase, thus increasing retention. Under these conditions, high coverage C18 columns are susceptible to a sudden loss in retention if the flow is stopped and restarted. This occurs when pressure is released from the column, causing the aqueous mobile phase to be expelled from the pores of the material (where a majority of the surface area lies)*2. If the pores remain inaccessible, the analytes pass through the column unretained. This phenomenon is referred to as pore dewetting. A shift in the void marker is also a clear indicator supporting this theory. Atlantis T3 columns were optimized with a balanced pore size to ligand density ratio to minimize pore dewetting.

*2 T.H. Walter, P. Iraneta and M. Capparella J. Chromatogr. A 2005, 075 177-183

AtlAntis t3

suPeRioR Column lifet ime at low pH

When exposed to strongly acidic mobile phases (i.e. < pH 2.0), traditional C18 columns can exhibit bonded-phase hydrolysis resulting in gradual loss of retention, loss of efficiency as well as change in selectivity. The T3 bonding process resists ligand cleavage by utilizing a trifunctional attachment of the C18 phase to the particle surface, thus providing exceptional column life at low pH.

0

10

20

30

40

50

60

70

80

90

20 hr exposure to 0.5% tfa at 60 C

% R

eten

tion

Loss

for M

ethy

l Par

aben

exC ePt ional Peak sHaPe

In addition to intermediate ligand density, the optimal balance of peak shape and retention exhibited by Atlantis T3 columns is a result of the low concentration of residual surface silanols. The T3 proprietary endcapping process is significantly more effective at reacting with the active sites of residual surface silanols than traditional trimethylsilane (TMS), resulting in brilliant peak shape and stability.

1 2 34

5

12

43,5

1

2

3,4

5

1 2

4

3

5

1 23

45

0 1 2 3 4 5 6 7 8 min

Atlantis T3

Agilent Zorbax SB-AqThermo

Hypersil Gold aQ

Phenomenex Synergi Hydro-RP

Shiseido Capcell PAK C18 AQ

Compounds:1. Thiourea2. 5-Fluorocytosine3. Adenine

4. Guanosine-5'-monophosphate5. Thymine

Atlan

tis T3

SunFi

re C 1

8

Pheno

mene

x Sy

nergi

Hydr

o-RP

Shise

ido Ca

pcell P

AK A

Q

Pheno

mene

x Sy

nergi

Polar

-RP

Agile

nt Zo

rbax E

clipse

Plus

Atlan

tis dC 1

8

Pheno

mene

x Aq

ua

YMC H

ydrosp

here

C 18

GL Sci

ences

Inert

sil O

DS-SP

YMC-P

ack O

DS AQ

[ ]7

AtlAntis t3

[ ]8

minimiZe Risk wit H dePendaBle ConsumaBle PeRfoRmanC e

The evolution of current good manufacturing practices (cGMPs) within regulatory agencies has encouraged drug and biologics industries to implement quality and risk management based principles throughout the pharmaceutical development process. With tight process control, Waters is uniquely positioned as an industry partner to minimize risk associated with variability caused by separation media.

Atlantis columns are manufactured in our cGMP, ISO 9001 and ISO 13485 certified facilities to assure separation scientists that results achieved today, will be repeatable and reproducible year after year.

Batch A 5 m

Batch B 3 m

Batch C 3 m

Batch D 5 m

Batch E 3 m

Batch F 5 m

Note: Column length (L) to particle size (Dp) ratio was scaled to hold resolution constant. Atlantis T3 columns were 4.6 x 150 mm, 5 m or 4.6 x 100 mm, 3 m

qualit y syst ems

Global organizations are narrowing the number of LC column choices available to their separation scientists by evaluating

a number of columns against a certain set of

performance criteria including selectivity, scal-ability, reproducibility, robustness and ruggedness. These harmonization efforts are an attempt to provide simplified method transfer throughout an organization, predictable and controlled consumable costs as well as reduce vendor diversity in efforts to minimize risk.

Waters has set the industry benchmark for batch-to-batch reproducibility. As a primary manufacturer of silica and hybrid particles, manufacturing processes are meticu-lously monitored and controlled to ensure consistent and complete material characterization over the lifetime of the product.

AtlAntis t3

t3 PeRfoRmanC e foR uPlC t eCHnology

ACQUITY UPLC HSS T3 columns utilize the same bonding and endcapping processes as Atlantis T3 HPLC columns, thus providing the same versatility and performance for diverse analyte mixtures, independent of your separation platform.

0 1 2 3 4 5 6 7 8 min

1

2

3

4

5

6

7

8

9

10

12

13

17 19 20

11

14

15

16

18

ConditionsLC System: ACQUITY UPLC SystemColumn: ACQUITY UPLC HSS T3, 2.1 x 100 mm, 1.8 mPart Number: 186003539Mobile Phase A: 0.15% CF3COOH in H2OMobile Phase B: 0.02% CF3COOH in 75:25 (v/v) ACN:MeOHGradient: Time %A %B 0 99.9 0.1 0.5 99.9 0.1 1.7 87.0 13.0 3.6 67.0 33.0 7.5 1.0 99.0 8.0 1.0 99.0 8.5 99.9 0.1 9.5 99.9 0.1 Flow Rate: 0.6 mL/minInjection: 1.0 LLoop Size: 2.0 LInject Mode: Partial loop with needle overfillColumn Temp.: 30 CDetection: UV @ 254 nmSampling Rate: 20 HzTime Constant: 0.2

Elution Order RT USP Resolution 1. 4-Aminophenol 0.858 - 2. Maleate 1.042 4.25 3. Fumarate 1.506 12.58 4. Phenylephrine 2.027 17.72 5. Acetaminophen 2.340 10.71 6. Phenylpropanolamine 2.778 14.17 7. Pheniramine 2.864 2.83 8. Doxylamine 2.939 2.62 9. Pseudoephedrine 3.016 2.5110. Pyrilamine 3.336 10.0811. Chlorpheniramine 3.622 8.9212. Brompheniramine 3.782 4.8813. Guaifenesin 3.938 4.1114. Acetlysalicylic acid 4.040 2.4215. 4-Nitrophenol 4.310 6.3616. 4-Chloroacetanilide 4.708 9.9317. Dextromethorphan 4.970 7.6918. Diphenhydramine 5.022 1.6419. Clemastine 5.997 30.9520. Ibuprofen 6.448 14.09

Peaks 1, 15 and 16 are impurities of acetaminophen

Reference: E.S. Grumbach, D.M. Diehl, J.R. Mazzeo "Quantitation of Over-The-Counter Cold Medications Using UPLC Technology" Waters Application Note 2007, 5 pp; search for 720002005EN on waters.com

sCalaBil it y aC Ross t eCHnology Plat foRms

Based on different particle substrates, Atlantis T3 and HSS T3 columns enable similar selectivity and retentivity to be achieved across HPLC and UPLC Technology platforms, enabling ease of method transfer across an organization.

t3 Bonding enables HPlC to uPlC transfer

5

1

2

3

4

6

0 1 2 3 4 5 6 7 8 9 min

7

0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 min

1

2

3

4

5

6

7

HPlC Atlantis T3

5

1

2

3

4

6

0 1 2 3 4 5 6 7 8 9 min

7

0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 min

1

2

3

4

5

6

7

80% Runtime Reduction

uPlC ACQUITY UPLC HSS T3

Compounds Retention USP Time (min) Resolution

1. Cytosine 2.77 -2. 5-Fluorocytosine 3.58 4.563. Uracil 3.93 1.714. 5-Fluorouracil 4.59 3.415. Guanine 5.92 7.766. Thymine 7.63 11.017. Adenine 8.39 4.70

Compounds Retention USP Time (min) Resolution

1. Cytosine 0.34 -2. 5-Fluorocytosine 0.44 5.023. Uracil 0.48 1.504. 5-Fluorouracil 0.56 3.175. Guanine 0.80 8.166. Thymine 1.25 14.657. Adenine 1.33 3.50T3 Bonding Allows for the Transfer of HPLC Methods to UPLC.

Similar Selectivities

[ ]9

[ ]12

HydRoPHiliC inteRaCtion CHRomatogRaPHy (HiliC)

HILIC is an alternative chromatographic technique that offers complementary selectivity to RP and often retains very polar species that cannot be retained by traditional means. Atlantis HILIC Silica columns were designed to retain very polar, organic molecules that are too polar to retain by RP*3. Unlike the highly aqueous mobile phases required for polar retention in RP separations, Atlantis HILIC Silica columns employ highly volatile (>80% organic) mobile phases which are ideal for mass spectrometry (MS) response and sensitivity. Additionally, direct compatibility with high organic SPE eluates dramatically increases the number of samples that can be handled, thus substantially increasing sample throughput.

*3 E.S. Grumbach et al LCGC N. Am. 2004, 22, 1010-1023

enHanC ed Ret ent ion foR PolaR ComPounds

HILIC retention mechanisms are a complex combination of partitioning, ion-exchange and hydrogen bonding, resulting in enhanced retention for polar analytes*3,4 . Atlantis HILIC Silica columns, used in combination with high organic (>80% acetonitrile) mobile phases, result in retention of analytes that are simply too polar to be retained by traditional RP chromatography.

*4 E.S. Grumbach et al J. Sep. Sci. 2008, 31, 1511-1518

0 1 2 3 4 5 min

0 1 2 3 4 5 min

NH

HN

O

OHNH2N

O

Reversed-PhaseRetention Factor = 0

HiliCRetention Factor = 1.1

Atlantis HILIC Silica

Allantoin

AtlAntis HiliC siliCA

t He standaRd foR Hil iC Column Ret ent ion

For the best combination of peak shape and retention, Atlantis HILIC Silica columns set the industry standard for HILIC separations.

1 2

1 2 3 4 min0

12

1

2

12

1 2 Atlantis HILIC Silica 3 m

XBridge HILIC 3.5 m

Phenomenex Luna HILIC 3 m

Tosoh TSKgel Amide-80 3 m

SeQuant ZIC HILIC 3.5 m

v0 = 0.37 min

v0 = 0.35 min

v0 = 0.29 min

v0 = 0.29 min

v0 = 0.34 min

[ ]10

1. Acetylcholine (Ach) 2. Choline (Ch)

ON+

O

HON+

[ ]12

AtlAntis HiliC siliCA

enHanC ed mass sPeCt Romet Ry ResPonse

Utilization of HILIC has grown in popularity, primarily due to the extensive adoption of mass spectrometry as a detector and the necessity of improving sensitivity for the quantitation of polar analytes. Unlike reversed-phase which utilizes high aqueous mobile phases to induce retention, HILIC employs an acetonitrile-rich mobile phase. This high organic mobile phase is easily desolvated, resulting in improved ionization efficiency and mass spectrometry response. For these reasons, HILIC has become a popular technique for bioanalytical laboratories.

100

%

2

1

0.5 1.0 1.5 2.5

1.92

1.53

2.0 3.0 3.5 4.0 4.5 5.0 min

0

100 1.84

1

2100

1 2 3 4 5 min0

%

0

%

1.84 2.181 2

2.18

0.5 1.0 1.5 2.52.0 3.0 3.5 4.0 4.5 5.0 min

Reversed-Phase0 50% ACN

HiliCAtlantis HILIC Silica

95 50% aCn

inC Reased samPle t HRougHPut

When employing solid-phase extraction (SPE) methods for sample clean-up, a high organic fraction is often used to selectively elute analytes of interest from the device. Before injecting this fraction onto a reversed-phase column, this organic fraction must first be evaporated then reconstituted with a portion of aqueous solvent. These two steps are often the lengthiest steps in an SPE procedure. With Atlantis HILIC Silica columns, the high organic fraction can be directly injected, thus eliminating the need for evaporation and increasing sample throughput.

1.49 1.84

0

100

0.50.0 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 min

%

21

N H

HO

O H

H3C

HOCH3

CH3H3C NH

OH

OH

1. Albuterol 2. Bamethan

1. Bamethan

2. Albuterol

HO

HN

OH

HO

HO

OH HN

[ ]11

Elute

75 L 60:40 IPA:ACN with 2% HCOOH

Wash 1

200 L 5% MeOH in H2O

Load

75 L spiked plasma 75 L internal standard with 2% NH4OH

Bronchial Dilator in Rat Plasma

Oasis HLB Elution Plate

Condition/Equilibrate

200 L MeOH/200 L H2O

Direct Injection of Eluate

Eliminate lengthy evaporation and reconsititution steps

[ ACQUITY UPLC SYSTem ACCeSSorIeS ]

[ ]12

PolAr retention strAtegy

guide to Retaining Polar Compounds

Atlantis T3

Insufficientretention?

Alternate separationtechnique, such as

ion pair orion exchange

Yes

Yes

Base Acid or Neutral

No No

Yes

No

Is the polar compound an acid, neutral

or base?

Atlantis T3or

XBridge C18

Can it beretained by

reversed-phase?

Sufficient massspectrometrysensitivity?

Atlantis HILIC Silicaor

XBridge HILIC

Can it beretained by

reversed-phase?

Yes

key:

atlantis t3 = low pH (pH 3), high aqueous (>95%)

xBridge C18 = high pH (pH 10), high aqueous (>95%)

atlantis HiliC silica = low pH (pH 3), 10 mM ammonium formate with 0.2% formic acid in 95% ACN:5% H2O

xBridge HiliC = mid pH (pH 5), 10 mM ammonium acetate with 0.02% acetic acid in 95% ACN:5% H2O

Atlantis Columns Applications Notebook, Literature Reference 720000472EN

Atlantis T3 and ACQUITY UPLC HSS T3 Columns Toss Sheet, Literature Reference 720001887EN

Topics in Liquid Chromatography: Part 1. Designing a Reversed-Phase Column for Polar Compound Retention White Paper, Literature Reference 720001889EN

XBridge Columns Brochure, Literature Reference 720001255EN

Preparative OBD Columns Brochure, Literature Reference 720002336EN

Interactive Waters Reversed-Phase Column Selectivity Chart www.waters.com/selectivitychart

Preparative OBD Column Calculator www.waters.com/prepcalculator

to obtain literature Pdf's, search for reference numbers on www.waters.com

Literature References

[ ]12

[ ACQUITY UPLC SYSTem ACCeSSorIeS ]

[ ]13 [ ]14[ ]13

Atlantis Analytical Columns

Dimensions Type Particle Size T3 HILIC Silica

1.0 x 50 mm Column 3 m 186003713 1860020031.0 x 150 mm Column 3 m 186003714 2.1 x 10 mm Guard 3 m 1860037561 18600200512.1 x 15 mm DC 3 m 1860020072.1 x 20 mm Guard 3 m 2.1 x 20 mm IS Column 3 m 186003715 2.1 x 30 mm Column 3 m 186003716 1860020092.1 x 50 mm Column 3 m 186003717 1860020112.1 x 100 mm Column 3 m 186003718 1860020132.1 x 150 mm Column 3 m 186003719 1860020153.0 x 20 mm IS Column 3 m 186003720 3.0 x 50 mm Column 3 m 186003721 1860020173.0 x 100 mm Column 3 m 186003722 1860020193.0 x 150 mm Column 3 m 186003723 3.9 x 20 mm Guard 3 m 1860037573 18600202133.9 x 50 mm Cartridge 3 m 3.9 x 100 mm Column 3 m 3.9 x 150 mm Column 3 m 4.6 x 20 mm Guard 3 m 1860037583 18600202334.6 x 20 mm IS Column 3 m 186003724 4.6 x 30 mm Column 3 m 186003725 1860020254.6 x 50 mm Column 3 m 186003726 1860020274.6 x 75 mm Column 3 m 186003727 4.6 x 100 mm Column 3 m 186003728 1860020294.6 x 150 mm Column 3 m 186003729 1860020311.0 x 50 mm Column 5 m 186003730 1860020041.0 x 150 mm Column 5 m 186003731 2.1 x 10 mm Guard 5 m 1860037591 18600200612.1 x 15 mm DC 5 m 1860020082.1 x 20 mm Guard 5 m 2.1 x 20 mm IS Column 5 m 186003732 2.1 x 30 mm Column 5 m 186003733 1860020102.1 x 50 mm Column 5 m 186003734 1860020122.1 x 100 mm Column 5 m 186003735 1860020142.1 x 150 mm Column 5 m 186003736 1860020163.0 x 20 mm IS Column 5 m 186003737 3.0 x 50 mm Column 5 m 186003738 1860020183.0 x 100 mm Column 5 m 186003739 1860020203.0 x 150 mm Column 5 m 186003740 3.0 x 250 mm Column 5 m 186003741 3.9 x 20 mm Guard 5 m 1860037603 18600202233.9 x 50 mm Cartridge 5 m 3.9 x 100 mm Column 5 m 3.9 x 150 mm Column 5 m 4.6 x 20 mm Guard 5 m 1860037613 18600202434.6 x 20 mm IS Column 5 m 186003742 4.6 x 30 mm Column 5 m 186003743 1860020264.6 x 50 mm Column 5 m 186003744 1860020284.6 x 75 mm Column 5 m 186003745 4.6 x 100 mm Column 5 m 186003746 1860020304.6 x 150 mm Column 5 m 186003747 1860020324.6 x 250 mm Column 5 m 186003748 186002033

Atlantis Columns Method Validation Kits

Dimensions Particle Size T3 HILIC Silica

4.6 x 150 mm 3 m 186003751 1860023154.6 x 150 mm 5 m 186003754 1860023144.6 x 250 mm 5 m 186003755 186002316

1 Requires Sentry Guard Holder WAT0979582 Requires Sentry Guard Holder 1860002623 Requires Sentry Guard Holder WAT046910

4 Requires Cartridge Fittings WAT037525 Intended Use

Particle Type Available Chemistries pH Range Maximum Rated Pressure Particle Size Pore Diameter/Volume Surface Area

Stationary Phase Characteristics

HPLC

Atlantis Silica T3, dC18, HILIC Silica

T3: 2-8; dC18: 3-7; HILIC Silica: 1-5 6000 psi [400 bar]

3, 5, 10 m 100/1.0 mL/g

330 m2/g

Atlantis Prep Columns Dimensions Type Particle Size T3 HILIC

10 x 10 mm Guard 5 m 1860036951 10 x 50 mm Column 5 m 186003691 10 x 100 mm Column 5 m 186003692 10 x 150 mm Column 5 m 186003693 10 x 250 mm Column 5 m 186003694 19 x 10 mm Guard 5 m 1860036992 1860039562 19 x 50 mm OBD Column 5 m 186003696 186003957 19 x 100 mm OBD Column 5 m 186003697 186003958 19 x 150 mm OBD Column 5 m 186003698 186003959 19 x 250 mm OBD Column 5 m 186004026 30 x 50 mm OBD Column 5 m 186003700 186003960 30 x 75 mm OBD Column 5 m 186003701 30 x 100 mm OBD Column 5 m 186003702 186003961 30 x 150 mm OBD Column 5 m 186003703 186003962 50 x 50 mm OBD Column 5 m 186004080 50 x 100 mm OBD Column 5 m 186004081 50 x 150 mm OBD Column 5 m 186004082 10 x 10 mm Guard 10 m 1860037061 10 x 150 mm Column 10 m 186003704 10 x 250 mm Column 10 m 186003705 19 x 10 mm Guard 10 m 1860037102 19 x 50 mm OBD Column 10 m 186003707 19 x 150 mm OBD Column 10 m 186003708 19 x 250 mm OBD Column 10 m 186003709 30 x 150 mm OBD Column 10 m 186003711 30 x 250 mm OBD Column 10 m 186003712 50 x 50 mm OBD Column 10 m 186004083 50 x 100 mm OBD Column 10 m 186004084 50 x 150 mm OBD Column 10 m 186004085 50 x 250 mm OBD Column 10 m 186004086

Description Part No.

10 x 10 mm Cartridge Holder 289000779 19 x10 mm Cartridge Holder 186000709Replacement o-ring 7.8 mm, 2/pkg 700001019Replacement o-ring 10 mm, 2/pkg 700001436Replacement o-ring 19 mm, 2/pkg 700001020

Purification and Isolation Cartridge Holders

1 Requires 10 x 10 mm Prep Guard Holder 289000779 2 Requires 19 x 10 mm Prep Guard Holder 186000709

orderINg INformATIoN

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[ ACQUITY UPLC SYSTem ACCeSSorIeS ]

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Dimensions Type Particle Size T3 HILIC

10 x 10 mm Guard 5 m 1860036951 10 x 50 mm Column 5 m 186003691 10 x 100 mm Column 5 m 186003692 10 x 150 mm Column 5 m 186003693 10 x 250 mm Column 5 m 186003694 19 x 10 mm Guard 5 m 1860036992 1860039562 19 x 50 mm OBD Column 5 m 186003696 186003957 19 x 100 mm OBD Column 5 m 186003697 186003958 19 x 150 mm OBD Column 5 m 186003698 186003959 19 x 250 mm OBD Column 5 m 186004026 30 x 50 mm OBD Column 5 m 186003700 186003960 30 x 75 mm OBD Column 5 m 186003701 30 x 100 mm OBD Column 5 m 186003702 186003961 30 x 150 mm OBD Column 5 m 186003703 186003962 50 x 50 mm OBD Column 5 m 186004080 50 x 100 mm OBD Column 5 m 186004081 50 x 150 mm OBD Column 5 m 186004082 10 x 10 mm Guard 10 m 1860037061 10 x 150 mm Column 10 m 186003704 10 x 250 mm Column 10 m 186003705 19 x 10 mm Guard 10 m 1860037102 19 x 50 mm OBD Column 10 m 186003707 19 x 150 mm OBD Column 10 m 186003708 19 x 250 mm OBD Column 10 m 186003709 30 x 150 mm OBD Column 10 m 186003711 30 x 250 mm OBD Column 10 m 186003712 50 x 50 mm OBD Column 10 m 186004083 50 x 100 mm OBD Column 10 m 186004084 50 x 150 mm OBD Column 10 m 186004085 50 x 250 mm OBD Column 10 m 186004086

2009 Waters Corporation. Waters, The Science of

Whats Possible, Atlantis, SunFire, UPLC, ACQUITY UPLC,

XBridge, Oasis, IS and OBD are trademarks of Waters Corporation.

All other trademarks are the property of their respective owners.

720002861EN March 2009 KK-WA

Austria and European Export (Central South Eastern Europe, CIS and Middle East) 43 1 877 18 07

Australia 61 2 9933 1777

Belgium 32 2 726 1000

Brazil 55 11 5094-3788

Canada 1 800 252 4752 x2205

China 86 10 8586 8899

CIS/Russia +7 495 3367000

Czech Republic 420 2 617 1 1384

Denmark 45 46 59 8080

Finland 09 5659 6288

France 33 1 30 48 72 00

Germany 49 6196 400600

Hong Kong 852 29 64 1800

Hungary 36 1 350 5086

India and India Subcontinent 91 80 2837 1900

Ireland 353 1 448 1500

Italy 39 02 265 0983

Japan 81 3 3471 7191

Korea 82 2 6300 4800

Mexico 52 55 5200 1860

The Netherlands 31 76 508 7200

Norway 47 6 384 60 50

Poland 48 22 833 4400

Puerto Rico 1 787 747 8445

Singapore 65 6273 1221

Spain 34 93 600 9300

Sweden 46 8 555 11 500

Switzerland 41 56 676 70 00

Taiwan 886 2 2543 1898

United Kingdom 44 208 238 6100

All other countries: Waters Corporation U.S.A. 1 508 478 2000

1 800 252 4752

www.waters.com

sales offices

T he quality management system of Waters manufacturing facilities in Taunton, Massachusetts and Wexford, Ireland complies with the International Standard ISO 9001:2000 Quality Management and Quality Assurance Standards. Waters quality management system is periodically audited by the registering body to ensure compliance.