Assay for an Antibiotic Resistance Enzyme

Carolina Kit

Timeline

Tuesday—Lecture,Review concepts of procedure

Thursday—Part 1

Part 2 (volunteer to serve as demonstrator—4 hours lab service)

Monday--handouts due

Prep. For lab—Assay for Anti-biotic resistance

2 days prior (Tuesday)--label tubes

control (8--15mL tubes)pAMP (8--15 mL tubes)penicillin-G (8--15 mL tubes)

--streak fresh plate of MM294 and MM294/pAMP (24 hours at 37 incubator)1 day prior (Wednesday)

--prepare cultures (1/group)—see teacher guide (24 hours at 37 incubator)--penicillin-G solution (store at -20 until use)--aliquot (8 groups)

• MM294 5ml overnight culture, label control• MM294/pAMP 5ml overnight culture, label pAMP• 5ml of 4mg/mL penicillin-G

On lab day 1 (Thursday)--test the reagents with phenol red

Lab day 2 (Monday)--demonstrator of spectrometer

Write-up

• Complete all aspects of the handout (50 points)

Staple these together. Drawings should be neat!