ASI Series 500 pumps - DataApex · ASISeries500pumps 3InstallationProcedure StarttheClaritystationbyclickingonthe icononthedesktop. Invoke the System Configuration dialog accessible

ASI SERIES 500 PUMPS

Clarity Control Module ENG

CodeRev M17080BDate 3272019

Phone +420251013400 DataApex Ltd

Fax +420251013401 Petrzilkova 258313

claritydataapexcom 15800Prague 5

wwwdataapexcom TheCzech Republic

Clarityreg DataApexreg and reg are trademarks of DataApex Ltd Microsoft reg and WindowsTM aretrademarks of Microsoft CorporationDataApex reserves the right to make changes to manuals without prior notice Updated manuals can bedownloaded fromwwwdataapexcom

Author zte

ASI Series 500 pumps Table of Contents

Contents1 ASI Series 500 pumps Control Module 12 Requirements 23 Installation Procedure 331 ASI Series 500 pumps communication 332 Clarity Configuration 4

4 Using the control module 741 Method Setup - LC Gradient 8411 Gradient Options 11

42 Method Setup - LC 1243 Method Setup - Event Table 1344 Method Setup - Advanced 1445 Hardware Configuration 1546 Device Monitor 16461 LC Control Manual Flow 19

47 DataApex UNI Setup 205 Report Setup 226 Troubleshooting 23

To facilitate the orientation in theASI Series 500 pumpsmanual and Clarity chromatography stationdifferent fonts are used throughout themanual Meanings of these fonts are

Instrument (blue text) marks the nameof thewindow to which the text refersOpen File (italics) describes the commands and names of fields in Clarity parameters that canbe entered into themor a window or dialog name (when you already are in the topic describingthewindow)WORK1 (capitals) indicates the nameof the file andor directoryACTIVE (capital italics) marks the state of the station or its part

The bold text is sometimes also used for important parts of the text and the name of the Claritystation Moreover some sections are written in format other than normal text These sections areformatted as follows

Note Notifies the reader of relevant information

Caution Warns the user of possibly dangerousor very important information

Marks the problem statement or trouble questionDescription Presents more detailed information on the problem describes its causes

etcSolution Marks the response to the question presents a procedure how to remove it

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ASI Series 500 pumps 1 ASI Series 500 pumpsControlModule

1 ASI Series 500 pumps Control Module

This manual describes the setting of the ASI Series 500 pumps and theVarian 212-LC pump The control module enables direct control of theinstrument over serial line

Fig 1ASI Series 500 pumps

Direct control means that the pump can be completely controlled from theClarity environment The Instrument method controlling the analysisconditions will be saved in the measured chromatogramsThe control is performed via the UNI Ruby control module and the ASISeries 500 pumps script

ASI Series 500 pumps 2 Requirements

2 RequirementsClarity Installation USB with appropriate control license allowed - LCControl (pn A24) for pumpsFree serial COM port in the PC

Note Modern computers usually have only one (if any) serial (COM) portinstalled To use more devices requiring the RS232 port the MultiCOMadapter (pnMC01) is available

Serial DB9F-DB9F cross cable (pn SK01) + DB9M-DB9M adapter

ASI Series 500 pumps 3 Installation Procedure

3 Installation Procedure31 ASI Series 500 pumps communication

The ASI Series 500 pumps is controlled by serial (RS232)communicationIt uses a DB9F-DB9M cross cable made up of a standard serial DB9F-DB9F cross cable (pn SK01) plus a DB9M-DB9M adapter

Fig 2 DB9F-DB9F cross cable

Fig 3 DB9M-DB9MAdapter

32 Clarity Configuration

Fig 4 How to Add ASI Series 500 pumpsmodule

Start the Clarity station by clicking on the icon on the desktopInvoke the System Configuration dialog accessible from the Claritywindow using the System - Configuration commandPress the Add button (① on 32 on pg 4) to invoke the Available ControlModules dialogYou can specify the search filter② to simplify the finding of the driverSelect the correct item and press the Add (③ on Fig 4 on pg 4) buttonEach device with already created UNI profile should have its own itemnamed accordingly in the Available Control Modules dialogThe DataApex UNI Setup dialog will appear

Fig 5 DataApexUNI Setup

Set the correct communication Port and click on the AutoDetect button toestablish communication with the deviceYou may fill in the custom Device Name

Caution When checked option EnableMinimumPressure Alarm pumpwill check ifpressure drops below value stated in Lower Pressure Limit [PSI] Thischeck is started after the pressure exceeds value stated in LowerPressure Limit [PSI] If pressure drops below the set value this check willresult in pumps shutdown and consequent complete loss ofcommunication withClarity

Note The DataApex UNI Setup dialog is described in detail in the chapterDataApex UNI Setup on pg 20

The ASI Series 500 pumps item ④ will appear in the Setup ControlModules list of the System Configuration dialog

Drag the appropriate item from the Setup Control Modules list on the leftside to the desired Instrument tab ⑤ on the right side ⑥ or click on the

button ⑦

ASI Series 500 pumps 4 Using the controlmodule

4 Using the control moduleAfter adding and setting up the new device one or more new tabs willappear in the Method Setup dialog depending on the type of theinstrument A new ASI Series 500 pumps section enabling the monitoringof the current pump state will be also created in the Device Monitorwindow

41 Method Setup - LC GradientThe Method Setup - LC Gradient tab is used for preparing and editing theinstrument method used by the ASI Series 500 pumps

Fig 6 Method Setup - LC Gradient

Gradient TableA table for setting the composition of the mobile phase and the overallflow rate as a function of time Operation is analogous to that ofspreadsheets (Excel Quatro Pro etc) To prepare the cell to receivevalues click it by the left mouse button the cell will highlight by dots A cellthat fails to highlight is not available for editingTime [min]Sets the time at which the ratio of flow rates and the overall flow ratecorrespond to the values entered in the corresponding row (Thesevalues vary continuously from one time to the next in a mannerensuring that the conditions specified in the next row are satisfied)XXX1 (4) []Represents the percentage of a component The designation XXX1-4is in fact replaced by the name of the component (items Solvent 1 - 4in the Gradient Options dialog) Should you enter a component valuesuch that the sum of all values exceeds 100 the percentage in thelast column is automatically adjusted if the percentage of the lastcompound is already zero the value of the currently entered

component is adjusted instead The flow rate of a compound iscalculated by multiplying the overall flow rate (indicated in the Flowcolumn) by the corresponding percentage divided by 100Flow [mlmin]Indicates the overall flow rate through the column The entered valueapplies to the time specified in the corresponding row The range forFlow values is 0 - 5 mlmin

GraphThe graph depicts the percentage of components as a function of timetogether with the overall flow rate Data are taken over from the GradientTable Changes effected in this table are immediately reflected in thegraph Legend in the header of the graph indicates the assignment ofcolors to individual components The assignment is fixed and individualcomponents are displayed in the graph from bottom to top The flow rate isdisplayed as a black lineThe graph has two vertical axes the axis on the left refers to the overallflow rate the one on the right to the mixing ratioParameters

Standby FlowSets the overall flow rate through the column in the STANDBY statereached after the last row of the table has been performed and thetime period defined in the Time to Standby field has passed Theduration of this state is defined by the Standby Time item The ratio ofindividual components in the respective STANDBY and IDLE states isgiven by the first row of the Gradient Table (the Initial row)Time to Standby [min]Indicates the time during which the flow rate and mobile phasecomposition changes continuously between the last values entered inthe table and the values defined by Standby Flow field and the Initialrow mobile phase compositionThis time is included in the analysis time (the Instrument is in theCONTROL state) In case when the Time to Standby is zero there isstep change from flow and components percentage specified on thelast row of gradient table to that specified for STANDBY stateStandby Time [min]The time during which the flow rate is maintained at Standby Flow This time is included in the analysis time (the Instrument is in theCONTROL state)

Idle StateAn item specifying the overall flow rate through the column outside theinstrument method The following options are possiblePump OffThe flow rates of all components are zero

Caution Be careful as this settingmaydamage the column in some cases

InitialThe flow rate is defined by the first row of the gradient table (the Initialrow)StandbyThe flow rate is the same as in the STANDBY mode and accordinglycorresponds to the value entered in Standby Flow fieldInitial - StandbyThe flow is defined by the first row of the gradient table (the Initial row)after the method is sent or by the value entered in the Standby Flowfield after the method finishes

The IDLE state comes into effect each time an Instrument is opened at theend or after abortion of an analysis by the Abort command and is alsomaintained after the Clarity program is shut downThe mixing ratio of individual components in both the IDLE and STANDBYstates is given by the first row of the Gradient Table (the Initial row)

Note There is a step change in the flow and components percentage from thevalues specified for the STANDBY state to those specified for the IDLEstate if the Idle State field is not set toStandby

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411 Gradient OptionsInvoke the Optionshellip button in the Method Setup - LC Gradient dialog toopen the Gradient Options dialog This dialog allows to set the customname for particular solvents to switch whether they are used or not in thegradient and to set the warning levels for pressure to prevent the damageto hardware

Fig 7 Gradient Options

Min PressureSets the minimum pressure for the given pump When pressure drops tothe set value the pump will shut down This prevents the solvent leakageThe range for Min Pressure values is 0 - 1231 MPaMax PressureSets the maximum pressure for the given pump When pressure reachesthe set value the pump will shut down This serves to prevent the damageto the pump when the column is blocked The range for Max Pressurevalues is 1 - 1241 MPa where the Max Pressure must also be at least 1MPa higher than the Min Pressure

Note Pressure limits are checked in the pump hardware Pressure checkingdoesnrsquot start immediately after the pump is started but with a few minutesdelay During this delay the pressure in chromatographic system canstabilize

Max Pressure for Set FlowSets the maximum pressure to be used in the Set Flow dialog in theDevice Monitor Initial value is 1 MPaSolvent 1 (4)It is possible to enabledisable particular solvent as well as to set customname to it

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42 Method Setup - LC

Fig 8 Method Setup - LC - AuxiliaryPump

This tab defines the properties of the ASI Series 500 pumps set asauxiliaryIdle FlowSets the flow of the pump used outside of the run The flow of the pumpthat should be used during the run can be defined in the Event TableLow Pressure LimitSets the minimum pressure for the given auxiliary pump When pressurereaches the set value the pump will shut down This prevents the solventleakageHigh Pressure LimitSets the maximum pressure for the given auxiliary pump When pressurereaches the set value the pump will shut down This serves to prevent thedamage to the pump when the column is blocked

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43 Method Setup - Event Table

Fig 9 Method Setup - Event Table

The Method Setup - Event Table tab can be apart from other things alsoused for switching the flow of the ASI Series 500 pumps set as auxiliarypump and switching the auxiliary output on the auxiliary pump

Note It is not possible to switch auxiliary output or change flow on a pump that ispart of the gradient from theEvent Table

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44 Method Setup - AdvancedThe Method Setup - Advanced tab serves for setting the usage of auxiliarysignals of the ASI Series 500 pumps

Fig 10 Method Setup - Advanced

The list of available auxiliary signals is shown in the table in the lower partof the dialog By checking the checkbox in the Store column for theparticular row the given auxiliary signal will be stored into the measuredchromatogram

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45 Hardware ConfigurationThe Hardware Configuration dialog (invoked by using the LC Statusbutton from the Method Setup - AS Method Setup - LC Method Setup - FCMethod Setup - ValvesMethod Setup - Aux - Properties dialog) displaysthe configuration of the ASI Series 500 pumps namely thecommunication type and its parameters

Fig 11 Hardware Configuration

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46 Device MonitorThe window with the pump status can be invoked by the Monitor - DeviceMonitor command from the Instrument window or using the DeviceMonitor icon

Device Monitor - LC MonitorFor gradient pumps the monitor consists of the section common to allpumps present in the gradient

Fig 12 Pump - DeviceMonitor

Stop FlowThe pumps can be stopped from this window using the Stop Flow buttonThis action will stop the pump only the analysis run will continue andmust be stopped or aborted separately by the Stop Abort button in thetoolbarSet FlowSets the desired total flow and solvent ratios in the opened Set Flowdialog Max Pressure allows to set a different pressure than set in theGradient Options on page 11 of a given method

Fig 13 Set Flow

Resume IdleReturns the pumps to IDLE state as defined in the appropriate field on theLC Gradient tab of the Method Setup dialog

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HoldResumeClicking on the Hold button will keep the current gradient conditions untilResume is clickedModify GradientOpens the LC Control Manual Flow dialog allowing to set custom flow andmobile phase composition disregarding the Gradient Table set in themethod The command is only available during the analysis run

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Device Monitor - Auxiliary PumpEach Auxiliary pump has its own device monitor displaying the actual flowand pressure on the device This separate monitor mimics the operationsof Stop Flow Purge and Resume Idle from the gradient Device Monitorbut can also be used during the analysis run

Fig 14 DeviceMonitor - Auxiliary pump

Note Please note that the separate Purge dialog is present for each auxiliarypump Auxiliary pumpsmust thusbe purgedmanually one at a time

Fig 15 Purge - Auxiliary pump

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461 LC Control Manual FlowThe LC Control Manual Flow dialog accessible through using the ModifyGradient hellip button from Device Monitor window allows the user to set acustom mobile phase composition and flow while the analysis is runningIt resembles the Method Setup - LC Gradient tab in functionality

Fig 16 LC ControlManual Flow dialog

The LC Control Manual Flow dialog is only available during the analysisrun When it is invoked and the OK button is pressed the originalGradient Table from the acquisition method is discarded and replaced bythe Gradient Table from the LC Control Manual Flow dialog Any suchoperation is recorded in the audit trail of the measured chromatogram

Caution After the analysis run which used manual flow changes ends the originalmethod is automatically sent to all controlled devices to make sure Claritystation returns to the originalmethod

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47 DataApex UNI SetupThe appearance of the DataApex UNI Setup dialog depends on thepresence of the selected Ruby Script - if the script is not present only theRuby Script field is visible

Ruby ScriptDisplays the selected Ruby Script The correct ASI500PUMPRB script forthe ASI Series 500 pumps instrument can be found in the UTILSUNI_DRIVERSASI subdirectory (accessible through the button) of theClarity installation folder (CCLARITYBIN by default)PortDefines the communication port used possible values dependent on thetype of communication of the device andor available ports in the PCAutoDetectIt is used for verifying the device communication over the serial portselected aboveInstrument NameAllows you to set the custom name of the instrument This name (enteredinto the Value column) will be used throughout the Clarity stationDo not Stop when Closing InstrumentThe pumps will not be shut down when closing the Clarity Instrument ifthis option is tickedAuxiliary PumpThe selected pump will be set as an auxiliary pump when ticking this boxEnable Minimum Pressure AlarmEnables or disables the minimum pressure check

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Lower Pressure Limit [PSI]Allows you to set the lower pressure limit on the method sent to the pumpClarity checks that the values set on the LC gradient do not go below theset limitUpper Pressure Limit [PSI]Allows you to set the upper pressure limit on the method sent to the pumpClarity checks that the values set on the LC gradient do not exceed the setlimit

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ASI Series 500 pumps 5 Report Setup

5 Report SetupThe pump section on the method report can be enabled by checking theInstrument Control checkbox on the Method tab of the Report SetupdialogAll of the parameters set in the Method Setup - LC Gradient dialog arereported as well as the custom Pump Name and other parameters set inthe DataApex UNI Setup dialog If the pump is configured as an AuxiliaryPump the parameters set in the Method Setup - LC dialog are reportedinstead

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ASI Series 500 pumps 6 Troubleshooting

6 TroubleshootingWhen the solution to a problem cannot be found easily a recording of thecommunication between Clarity and the pump will significantly helpDataApex supportThe data recording can be enabled by adding or amending theCOMMDRVINI file in the Clarity installation directory (CCLARITYCFGby default) The file can be edited in any text editor (eg Notepad) Thefollowing section should be edited or added

[COM1]echo=ontextmode=onfilename=CommDrvCOM1_Dtxtreset=off

Note Instead of COM1 type the communication port used to communicate withthe This port number is displayed when the LC Status button in theMethod Setup - LC dialog is invoked

Note D (or d) in the filename parameter means that the log will be createdseparately for each day The reset=off parameter disables deleting thecontent of the log each time the station is started during the same day

The created TXT files will be of great help in the diagnosis of notdocumented errors and communication issues

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2 Requirements

3 Installation Procedure

31 ASI Series 500 pumps communication


4 Using the control module

41 Method Setup - LC Gradient

411 Gradient Options



44 Method Setup - Advanced

45 Hardware Configuration

46 Device Monitor

461 LC Control Manual Flow

47 DataApex UNI Setup

5 Report Setup

6 Troubleshooting

Clarityreg DataApexreg and reg are trademarks of DataApex Ltd Microsoft reg and WindowsTM aretrademarks of Microsoft CorporationDataApex reserves the right to make changes to manuals without prior notice Updated manuals can bedownloaded fromwwwdataapexcom

Author zte

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Documents

ASI Series 500 pumps - DataApex · ASISeries500pumps 3InstallationProcedure StarttheClaritystationbyclickingonthe icononthedesktop. Invoke the System Configuration dialog accessible