8.25.16MUNDIE COURTYARDFRED HUTCH CAMPUS

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ART WALKART & SCIENCE

Wounding Supernova

PRESENTS

ART WALK

Making what’s invisible visible

in how we treat diseases,

from finding a vaccine to

curing cancer. Discover the

strikingly beautiful images

our Hutch researchers have

captured. These scientific

images exceed their role as a

medium for communicating

information and contain

the artistic qualities that

transform them into objects

of beauty and art.

Kinetochores Structure Image by Julia Torvi / Biggins Lab*

During each phase of mitosis, a type of cell division, coordinated cell activities help DNA divide evenly between two new daughter cells. In metaphase (bottom right), chromosomes line up. In anaphase (center), chromosomes are pulled apart by microtubules (red), which attach to the chromosomes’ kinetochores (green). By early telophase (top left) two daughter cells begin to form.

Crystal Packing Geometries of Designed Toroids Image courtesy of the Bradley Lab

Fred Hutch scientists are developing new computer algorithms to precisely design proteins from scratch, with many potential therapeutic and diagnostic applications. The challenge is to determine the three-dimensional structures of these designed proteins, which are far too small to see under a microscope. One solution is to coax these designer molecules into forming crystals like the one shown here, whose structures can then be probed by powerful X-ray beams.

Epithelial Cells Image by Anjali Teckchandani / Cooper Lab*

These human epithelial cells growing on glass are stained to show sites of adhesion to the glass (blue and red) and fibers (green) containing actin, a protein necessary for many cellular functions.

Mouse Brain Image by Yves Jossin / Cooper Lab*

This section of the cerebral cortex of a developing mouse brain shows cells (blue) migrating outward from a tube-shaped structure (bottom right) into the structure shown. Some cells (green) have migrated normally to their place under the outer surface; others express a gene that stalls their movement (red).

Immunotherapy #1 Image by Eric Holland / Holland Lab

Human, patient-derived glioblastoma cells (green) transplanted into the brains (red) of immune-compromised mice, recapitulate many hallmark features of the human disease.

Immunotherapy #2 Image by Eric Holland / Holland Lab

Patient-derived glioblastoma cells (green) co-mingling and interacting with stromal astrocytes (red) within the brain of a mouse.

Sunrise Image courtesy of the Paddison Lab

This is a microscopic image of a single dividing human brain tumor cell derived from a patient with glioblastoma, or brain cancer. Observe the highly organized mitotic spindle (red), which helps provide the pulling force required to properly segregate DNA chromosomes during cell division. The red lines emanating from two spindle poles are called tubulins, which act as guides for the transport of whole chromosomes. The multi color, punctate spots in the middle of the spindle are kinetochores that promote physical attachments between the chromosomes and the mitotic spindle. Dr. Patrick Paddison’s lab has found that kinetochore regulation is altered in brain tumors. As a result, their work has suggested new therapeutic strategies for brain tumors and likely many other types of cancer.

Green Glow Image by Lixia Bai / Rohrschneider Lab

In this image of a breast tumor, blue highlights the tumor cells’ nuclei, which contain their DNA. Cancer stem cells scattered through the tumor glow green.

Vagus Image by Gabby Barsh / Moens Lab

The vagus motor neurons at the base of the brain innervate our larynx and pharynx for speech and swallowing, as well as our internal organs to control heartbeat, breathing and digestion. This image shows the vagus motor neurons in a transgenic zebrafish, which express a green-to-red photoconvertible fluorescent protein, Kaede (green). The Kaede in a subset of the neurons has been photo-converted using ultraviolet light (magenta). The Moens Lab uses these genetic tools to discover whether motor neurons in the brain are organized in a spatial representation of their peripheral targets, and how this map forms during embryonic development.

Snapshot of Motor Neurons Image by Paul Grant / Moens Lab

Cross section of a zebrafish hindbrain showing motor neurons that innervate the jaw muscles (green), commissural interneurons (blue) and the basement membrane of the neural tube (red). The Moens Lab discovered that the basement membrane serves to constrain and guide the migration of motor neurons during brain development.

14h - Zebrafish Series #1 Image by Andrew Mathewson / Moens Lab

A zebrafish embryo at 14 hours of development. The forming eye is visible at 12:00 and developing musculature is visible as a series of segmental blocks, the somites, between 5:00 and 8:00. This embryo expresses GFP (green fluorescent protein) in the forming gut, or endoderm. The Moens Lab uses this transgene at a later developmental stage to study how cell polarity in the central nervous system is established.

Day 5 - Zebrafish Series #3 Image by Adam Miller / Moens Lab

Part of the spinal cord of a zebrafish embryo at five days of development. Axons of brainstem neurons that course along either side of the spinal cord (parallel red tracks) make periodic contacts with crossing axons (thin red lines criss-crossing the tracks) and when they do, they form a synapse (yellow spots). The Moens Lab uses this easily visualized synapse to discover how genes function to build neuronal circuits that govern behavior.

Ovary and Tumor 10x Image by Kristin Anderson / Greenberg Lab

Female reproductive tract tissues from a mouse with ovarian cancer. This is an ovarian tumor (thick red region) growing on an ovary magnified at 10x.

Uterine Horn 20x Image by Kristin Anderson / Greenberg Lab

Female reproductive tract tissues from a mouse with ovarian cancer. This is a uterine horn tumor (thick red region) growing on an ovary magnified at 20x.

Yeast Cells Image by Babak Momeni / Shou Lab

Yeast cells that are engineered to engage in cooperation and cheating reveal self-organization in favor of cooperators in microbial communities. Fluorescently labeled red and green cells cooperate by providing necessary nutritional benefits to each other. Blue cells cheat by consuming the benefits produced by green cells without reciprocating.

Ovarian Tumor 40x Image by Kristin Anderson and Ingunn Stromnes / Greenberg Lab

Mouse ovarian cancer expression of proteins being targeted by immunotherapy.

Wounding Supernova Image by Mitsutoshi Nakamura / Parkhurst Lab

Cell wounding is a common event in the life of many cell types, and the capacity of a cell to repair day-to-day wear-and-tear injuries, as well as traumatic ones, is fundamental for maintaining tissue integrity. Actin filaments, structural components of the cell, are rapidly recruited to wounds to provide a scaffold for this repair. The faster the actin moves toward the wound (center) the more yellow it appears.

Actin Image courtesy of the Parkhurst Lab*

A cell’s shape, structure and movement are controlled by its cytoskeleton — a scaffold made of actin filaments (green) and microtubules (red). This image provided the first evidence of a protein (yellow) that can link the two components to coordinate their roles. Understanding the cytoskeleton sheds light on how cancer grows and spreads throughout the body.

Making a Fruit Fly Embryo Image courtesy of the Parkhurst Lab*

Different stages of ovarian development in the fruit fly (roughly five hours apart). Each stage is a separate unit, called an egg chamber, consisting of the future embryo and its 15 support cells (blue nuclei ringed in red) surrounded by a sheath of mother cells (yellow).

SyphCell1 Image by Raymond Liu / Parkhurst Lab*

Building tissue and forming organs requires cells to move and change their shape. To do so, they rely on long, stiff strings of molecules known as the cytoskeleton. These molecules push cells’ edges outward, providing form and, when focused in just the right spot, forward momentum. Tumor cells can hijack these same molecules, using them to grow abnormally, invade neighboring tissues and spread throughout the body. Dr. Susan Parkhurst’s team studies the proteins that control the size and placement of the internal “skeleton” of cells. This image shows a close-up view of several cells with their cytoskeletons (bright green). The cells’ nuclei (blue) house their DNA.

Nop60 Image courtesy of the Parkhurst Lab

Cancer results from changes to the genetic blueprint in our cells. We are born with some of these changes while others accumulate over the course of our lives. Dr. Susan Parkhurst’s lab investigates ways to treat individuals born with genetic changes known to contribute to cancer development. One method involves inducing a second genetic change which, added to the first, will return the cell to a “normal” condition. This image shows the nucleus (blue) of a normal cell (top). The pink highlights a protein found within the nucleus. Scientists removed a cancer-related gene called myc from the cell on the left, which caused the protein to be under produced. They removed a different gene from the cell on the right, which caused the protein to be over produced.

Cerebellum Image by Dr. Libing Feng / Cooper Lab

In this image of the cerebellum from a normal mouse brain, small cells called granule neurons are shown in blue, and intricately branched neurons called Purkinje (purr-KIN-jee) cells are shown in green.

Rac1 Image by Youn Na / Cooper Lab*

The rainbow of colors in these mouse nerve cells show the activity of a protein called Rac1. Red indicates high activity, while yellow, green, blue, indigo and violet indicate ever-decreasing activity. Note the high activity of Rac1 in the nucleus and in long, thin processes extending from the ends of the growing nerve-cell branches.

Series of Immature Red Blood Cells Image by Eyayu Belay / Torok-Storb Lab**

A central macrophage coordinates the maturation of a series of immature red blood cells. The final maturation step involves the extrusion of their smooth round nucleus. Two extruded nuclei are seen on the left.

Brave New World Image courtesy of the Ghajar Lab

When nuclei are colored blue, the lymph node takes on the appearance of a tiny, intricate world, an apt description for a primary site of immunity generation. Large blood vessels (red) feed local cells while lymphatic vessels (green) percolate lymph fluid through net-like sinuses for immune cells to sample. Yellow is generated when the red- and green-emitted light overlap, indicating the presence of two or more kinds of cells.

Lymph Node’s Day in the Sun Image courtesy of the Ghajar Lab

Blood and lymph, the fluid that bathes tissues, come together at the crossroads known as a lymph node. Each node is a busy hive of immune cells actively monitoring these fluids for signs of infection. Many blood vessels (cyan) and lymphatic sinuses (magenta), contrasted with DNA (yellow), are apparent in this falsely-colored cross-section of a whole node.

Bacteria-Specific Immune Response in the Spleen Image by Nicholas Maurice / Prlic Lab

This photo depicts immunofluorescence staining of a mouse spleen. Listeria monocytogenes (green) is a bacteria that grows inside of cells and can be used as a model to understand how T cells respond to infection. In red are T cells that specifically recognize and kill Listeria-infected cells. Clustering around infected cells is a finely orchestrated mechanism to clear the infection and reduce collateral damage. This infection occurs primarily in the white pulp of the spleen, which is surrounded by macrophages (blue).

Lentiviral Targeting Image by Dr. Slobodan Beronja / Beronja Lab

The image illustrates our lentiviral targeting methodology to modify mouse skin and its appendages. It shows hair follicles marked by E-cadherin (epithelial cell-cell adhesion molecule) in cyan, and successfully targeted cells are marked in red.

Protein Drug Design (3D Image) Image by Zachary Crook / Olson Lab

Taking inspiration from nature, the Olson Lab is using small drug-like proteins to tackle diseases that have so far evaded conventional treatments. The lab can even use computer-assisted design to model target binding and predict changes that can be made to improve the drug.

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Image creation supported by the Scientific Imaging Shared Resource

Image creation supported by the Electron Microscopy Shared Resource

Binucleated Cell Image by Kristin Robinson / Galloway Lab*

Human papillomavirus (HPV) E6 and E7 proteins cause abnormal cell divisions as shown here. This is an image of a binucleated cell expressing HPV 5 E6. Actin (green) outlines the cell; dapi (blue) stains the two nuclei and tumor-suppressor protein (red).

Wounding Supernova

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