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Source: Abberior Instruments
Sample preparation
Applications
On-site demo, 25/1 to 12/2/2021
Plateforme d’imagerie cellulaire MIPSIT
Faculté de Pharmacie de Châtenay-Malabry
Source: Abberior Instruments
Company History
Nobel Prize for
Stefan W. Hell
Company
formation
2012 2013 2014 2015 2016 2017 20192018 2020
>300 publications
>50 employees
~35 years
>50% PHD
>120 installed systems
Source: Abberior Instruments
Abberior Instruments
STED measurement
Source: Abberior Instruments
Incredibly compact
Converts an epifluorescencemicroscope with a C-mount toconfocal and STED microscope
2D STED down to 35nm resolution
Beams are aligned by design
Easy-to-use software
Demo system configuration
PC and monitor
Scan head
Z-Piezo
Supply Unit
Demo system configuration
EpifluorescenceUpright base (no DIC)
- Demo objectives
For epifluorescence – confocal - STEDZeiss 100x objective, oil immersionNA 1.46FWD: 100m
For epifluorescence ( - confocal)Zeiss 20x objective, airNA 0.45FWD: 510m
- Demo Sample holder
confocal
Nups, Actin, Mitos 5 µm
Source: Abberior Instruments
Exc
Dyes
Channels for STED imaging Channels for confocal imaging
- dyes
488561640 405
Source: Abberior Instruments
488
Exc
Dyes
561640
- dyes
405
STAR ORANGE
STAR 580
STAR 600
Alexa 594
Atto 594
Atto 590
Cy3
STAR RED
STAR 635P
STAR 635
Atto 647N
Atto 633
Alexa 647
Cy5
STAR 488
Alexa 488
Oregon Green
Atto 488
FITC
STAR470SXP
STAR520 SXP
Live 580
Atto 590
SiR GFP
YFP
DAPI
Hoechst33258
Alexa 405
CFPliveimaging
fixedimaging
Channels for STED imaging Channels for confocal imaging
Source: Abberior Instruments
Culture chambers and slides
glass bottom petri dishes, slides, chamber slides…
glass thickness No #1.5, i.e. 0.16 to 0.19mm
plastics substrates – STED might not work
gridded cover slips groves may affect the focus
example
Source: Abberior Instruments
Mowiol / DABCO
Abberior mount solid
Prolong Gold antifade
Prolong Diamond antifade
Rotimount
...
buffered Glycerol/ DABCO
Abberior mount liquid
…
Hardening embedding media Not hardening embedding media
2D STED 2D STED with better preservation of 3D
morphology
- mounting media
See white paper: Labelling for STED
https://abberior-instruments.com/knowledge/white-papers/
Source: Abberior Instruments
- Antibody staining
Abberior kit
STAR RED anti-mouse
STAR RED anti-rabbit
STAR ORANGE anti-mouse
STAR ORANGE anti-rabbit
Abberior mount solid(heat in water bath at 70°C until
melted, cool down at 37°C, apply)
Abberior mount liquid
https://abberior-instruments.com/knowledge/white-papers/
https://abberior-instruments.com/knowledge/protocols/
Source: Abberior Instruments
On-site demo, 25/1 to 12/2/2021Plateforme d’imagerie cellulaire MIPSITFaculté de Pharmacie de Châtenay-Malabry
Contacts:
Valerie Nicolas, [email protected]
Frédéric Eghiaian, [email protected] 48 45 05 19
Achat de sondes:
www.abberior.com
2-color STED, 4-color confocal <50nm resolution in a few clicks
