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APPENDIX- 1
LESSON TRANSCRIPTS FOR WEB SITE LEARNING
LESSON - 1
PHOTOSYNTHESIS The objectives of the lesson:
1. The pupil acquires the knowledge of photosynthesis.
2. The pupil understands the significance of photosynthesis.
3. The pupil analyses photosynthesis as the important biological process.
4. The pupil organizes, evaluates and makes inferences on the importance of
photosynthesis.
Anticipatory set Or
Introduction
In the earlier class itself you have learnt about
Photosynthesis. Here we can learn about the significance
of photosynthesis and analyse why photosynthesis has
considered as the significant biological process required
for human existence.
Input Or Procedure
Here the lesson is prepared in different phases:
(1). The teacher presents the lesson in small steps.
(2). The teacher guides the students to learn the lesson
With divergent questions.
(3). The teacher explains the questions through guided
Practice.
(4). Students learn the lessons through thought -
Provoking questions without the help of the teacher.
Modelling
Autotrophic plants prepare their own food material
through the process called Photosynthesis.
Photosynthesis is the process of synthesis of
carbohydrates from carbondioxide and water in the
presence of sunlight and chlorophyll.
It is the most important synthetic process on earth. Here
the solar energy is fixed in the form of chemical energy
ie; the plants have the capacity of capturing light energy
from the sun and converting it into chemical energy.
Here the kinetic energy of sunlight is transformed in to
chemical energy and stored in the carbohydrate
molecules.
This chemical energy is used for the growth and
development of all forms of life in the universe.
Approximately 90% of the world’s photosynthesis is
carried out by marine algae and fresh water algae and all
animals depend on plants and their source of energy.
Photosynthesis is considered as the natural physio-
biological process of the world on which the existence of
life in earth depends. So photosynthesis is the single
most important biological process required for human
existence.
1. Photosynthesis is the process of synthesis of _______.
(Carbohydrate, Fat)
2. During photosynthesis ________ energy is converted
Into ___________ energy. (Light energy-Chemical energy, Sound energy- Checking for
understanding Chemical energy) 3. 90% of photosynthesis is carried out by ____________
and _______________.
(Marine algae & Fresh water algae, Animals &
Zooplankton)
4. The natural physio-biological process of the world is
___________.
(Photosynthesis, Respiration)
1. Photosynthesis is process of synthesis of
carbohydrate from Co2 and water in the presence
of sunlight and chlorophyll.
2. During photosynthesis light energy is converted
into Guided practice chemical energy.
Here the light energy from the sun is taken not the Sound energy.
3. 90% of the photosynthesis is carried out by
marine and fresh water algae.
4. The natural physio-biological process of the world is
Photosynthesis.
Independent practice
And Closure of the
Lesson
Answer the following questions by yourself. If you want to check whether it is correct or wrong, you can go to another file and see whether your answers are correct.
1. Why photosynthesis is considered as a significant
process?
2. What is Photosynthesis?
3. During photosynthesis which energy is converted
into chemical energy?
4. Which is the most important biological process
required for human existence?
CORRECT ANSWER FOR LESSON – 1
1. The photosynthesis is the most important synthetic process on earth.
Here the solar energy is fixed in the form of chemical energy and stored in
the carbohydrate molecule. This chemical energy is used for the growth
and development of all forms of life in the universe.
2. Photosynthesis is the process of synthesis of carbohydrate from carbon
dioxide and water in the presence of sunlight and chlorophyll.
3. During photosynthesis light energy is converted into chemical energy.
4. Photosynthesis is the most important biological process required for human existence.
LESSON - 2
CHLOROPLAST – THE SITE FOR PHOTOSYNTHESIS The objectives of the lesson:
1. The pupil understands the importance of chloroplast in photosynthesis.
2. The pupil identifies the structure of leaf and its importance.
3. The pupil analyses the importance of photosynthetic apparatus.
4. The pupil differentiates the different shapes of chloroplast.
5. The pupil syntheses the knowledge that size and shape of chloroplast
varies from species to species.
Anticipatory set or
Introduction
The students understand the structure of a leaf and the
arrangement of Chloroplast with the help of a diagram.
The students will be able to Understand the importance of
chloroplast in the process of Photosynthesis. The students
learn the various size and shape of chloroplast with the
help of diagrams.
Input or
Procedure
Teacher presents the lesson in different steps:
(1). Teacher explains the content in series of small steps.
(2). Teacher checks the understanding of students with
the help of multiple choice questions.
(3). Teacher explains the content once again through
guided practice.
(4). Teacher makes a closure of lesson through
independent practice.
Photosynthesis is the process of synthesis of carbohydrate
from Co2 and water in the presence of sunlight and
chlorophyll.
6CO2 + 12H2O C6H12O6 + 6H2O + 6O2 Here the photosynthesis takes place in the green parts of
the plant body such as leaves, green stems and in green
floral parts etc. In leaves the photosynthesis takes place
in the mesophyll tissues.
Structure of Leaf
Modelling
In the structure of a leaf the green tissues seen in
between the upper and lower epidermis is the mesophyll
tissue. The mesophyll is differentiated into palisade
parenchyma and spongy parenchyma. Both these
parenchyma cells contain the green plastid-
CHLOROPLAST.
These chloroplasts are the characteristic structure
of green plants. The chloroplasts are green due to the
presence of green pigment chlorophyll. Here the
chloroplasts are considered as the seats of
photosynthesis, they synthesis starch by using CO2 and
sunlight. So chloroplast is considered as the
photosynthetic apparatus.
The chloroplasts are distributed homogeneously in
the cytoplasm of plant cell. Their diameter is about 5
to 8 µ.
The size of the chloroplast varies from species to
species. In green algae, the chloroplast has various
shapes, example.,
Ribbon shaped (Spirogyra)
Star shaped (Zygnema)
Girdle shaped (Ulothrix)
Disc shaped (Caulerpa)
Reticulate shaped (Oedogonium)
Cup shaped (Chlamydomanas)
1. Photosynthesis takes place only in _____________. (Green parts, Flowers, Roots)
Checking for understanding
2. The green plastids seen in the cytoplasm are called
_________________.
(Chloroplast, Palisade tissue, Vacuoles )
3. ____________ is considered as the photosynthetic
apparatus.
(Mesophyll tissue , Flowers , Chloroplast )
4. The diameter of the chloroplast varies from ________.
(5-10 µ, 5-8 µ, 5-6 µ)
5. The shape of chloroplast in chlamydomonas is
______________.
(Star shaped, Girdle shaped, Cup shaped).
Guided practice
1. Photosynthesis takes place only in the green
parts of the plant body. For photosynthesis
sunlight and chlorophyll is essential. Chlorophyll is
seen in the green parts of the plant body.
2. In the leaves photosynthesis takes place in the
mesophyll tissues. These mesophyll cells contain
the green plastid - chloroplast.
3. Chloroplast synthesis starch by using CO2, Water
and Sunlight. So chloroplast is considered as the
photosynthetic apparatus.
4. The diameter of chloroplast varies from 5 to 8 µ.
5. The shape of chloroplast in chlamydomonas is cup
shaped. Star shaped in Zygnema and girdle
shaped in Ulothrix.
Answer the following questions by yourself. If you
want to check whether it is correct or wrong , you can go to another file and see whether your answers are correct.
Independent practice
1. Why chloroplast is considered as the photosynthetic
apparatus? And Closure of the
Lesson 2. Why photosynthesis takes place in the green parts
of the plant body?
3. Why photosynthesis doesn’t takes place in the roots
of the plants?
CORRECT ANSWER FOR LESSON – 2
1. Chloroplast are the characteristic structure of green plants they synthesize
starch by using co2, water and sunlight. So chloroplast is considered as
the photosynthetic apparatus.
2. Only in the green parts of the plant body chloroplast are seen. Only in the
presence of chloroplast photosynthesis takes place. So photosynthesis
takes place only in the green parts of the plant body.
3. In roots chloroplast are absent so photosynthesis doesn’t takes place.
LESSON - 3
STRUCTURE OF CHLOROPLAST The objectives of the lesson:
1. The pupil understands the structure of chloroplast.
2. The pupil explains the importance of chloroplast.
3. The pupil analyses the role of chloroplast in the process of photosynthesis.
4. The pupil syntheses the knowledge about the functions of chloroplast.
Anticipatory set or
Introduction
The teacher presents the lesson structure of chloroplast
with the help of a diagram. The students try to learn the
lesson using the diagram.
Input or Procedure
Teacher presents the lesson in different steps:
(1). The lesson is presented with the help of a diagram.
(2). Small questions are given for checking
understanding.
(3). Answers for the questions in the previous step is
given.
(4). Independent practice is given with the closure of the
Lesson.
(5). Correct answers for the questions in the independent
Practice is given.
Now we can see the structure of chloroplast.
Structure of Chloroplast
Modelling
The chloroplast is surrounded by two unit membrane.
Each membrane is lipo-proteinaceous in nature. The
space in between two membranes is known as
periplastidial space. The space inside the chloroplast is
filled with a proteinaceous fluid called Stroma.
The stroma contains DNA, Ribosome’s and Enzymes for
carbon assimilation. The enzymes found in stroma are
capable of utilizing ATP and NADPH2 to produce
carbohydrates.
A number of membrane bound, sac like structure are
embedded in the stroma. The structures are called the
Thylakoids or Lamellae. The thylakoids are seen in
groups. Each group is known as Granum. Each
thylakoid in a granum is called the grana thylakoid.
The thylakoid which interconnects the adjacent grana is
called the stromathylakoid or fret or intergranal thylakoid.
Each thylakoid is also a double membrane-bound
structure. The green pigment molecules, chlorophyll are
seen as units inside the thylakoids. In the process of
photosynthesis there are two types of reaction ie; Light
reaction or Photochemical reaction and Dark
reaction or Biosynthetic reaction.
Each smallest unit of pigment molecules in the thylakoids
which is capable of carrying out the light reaction is said
to be Photosynthetic unit or PSU or Quantasomes.
The granum is the site for photochemical reaction and the
stroma is the site for dark reaction in photosynthesis.
These chloroplast are located at the outer margins ie;
periphery of the cytoplasm with their broader surfaces
parallel to the cell wall of the mesophyll cells.
Checking for understanding
1. Chloroplast membrane is made up of _____________.
(Lipoprotein, Cellulose, Chitin)
2. The space inside the chloroplast is filled with a
protienaceous fluid called _____________.
(Grana, Stroma, Thylakoid)
3. The number of membrane bound sac like structure
Embedded in the stroma are _______________.
(Grane, Thylakoid or Lamellae, Stroma thylakoid)
4. The site for light reaction is _________________.
(Grana, Stroma)
5. The site for dark reaction is _________________.
(Grana, Stroma).
6. Each small unit of pigment molecule capable of
carrying out photosynthesis are called ________
or ____________.
(Stroma or Grana, Stroma thylakoid or Grana
thylakoid, Photosynthetic unit of Quantasome)
Guided Practice
1. Chloroplast membrane is lipo-proteinaceous in
nature.
2. The space inside the chloroplast is filled with a
proteinaceous fluid called the stroma. The stroma
contains DNA, Ribosomes and Enzymes for carbon
assimilation.
3. The number of membrane bound-sac like structure
embedded in the stroma is called Thylakoid or
Lamellae. The thylakoid are seen in groups, each
group is known as Granum. Each thylakoid in a
granum is called Grana thylakoid and the
thylakoid which interconnects the adjacent grana is
called Stroma thylakoid or Fret or Intergranal
thylakoid.
4. The site for light reaction or photochemical
reaction is Granum.
4. The site for dark reaction or biosynthetic reaction
is Stroma.
6. Each small unit of pigment molecule capable of
Carrying out photosynthesis is called
photosynthetic unit of quantosomes.
Answer the following questions by yourself. If you want to check whether it is correct or wrong , you can go to another file and see whether your answers are correct.
Independent
practice 1. Describe the structure of chloroplast. And 2. What are quantasomes? Closure of the
Lesson 3. How many types of reactions are in the process of
photosynthesis? Name them?
4. Why light reaction takes place only in the granum
of chloroplast?
5. Define Fret.
6. Why chloroplasts are located at the periphery of the
cytoplasm?
CORRECT ANSWER FOR LESSON – 3
1. The chloroplast is a two unit membraneous structure. Each membrane is
lipo-proteinaceous in nature. The space in between the two membranes is
known as periplastidial space. The space inside the chloroplast is filled
with a proteinaceous fluid called the stroma . The stroma contains DNA,
ribosomes and enzymes for carbon assimilation.
A number of membrane bound sac like structure are embedded in the
stroma called thylakoids or lamellae. The thylakoid are seen in groups.
Each group is called granum. Each thylakoid in a granum is called
grana thylakoid and the thylakoid which interconnect the adjacent
granum is called stroma thylakoid or fret or intergranal thylakoid.
2. The green pigment chlorophyll are seen as units inside the thylakoid. Each
small unit of pigment molecules capable of carrying out photosynthesis is
called quantasomes or photosynthetic unit.
3. There are two types of reaction in the process of photosynthesis they are
light reaction or photochemical reaction and dark reaction or biosynthetic
reaction.
4. For light reaction photochemical unit or quantasomes are required and
these quantasomes are seen in the granum. So light reaction takes place
in the granum of chloroplast.
5. The thylakoid which interconnects the adjacent granum is called the
stroma thylakoid or fret or intergranal thylakoid.
6. Chloroplasts are located in the periphery of the cytoplasm because it helps in
the diffusion of Co2 required for photosynthesis and in the easy harvesting
of solar energy.
LESSON - 4
PHOTOSYNTHETIC PIGMENT – CHLOROPHYLLa The objectives of the lesson:
1. The pupil tries to understand the importance of pigments in
photosynthesis.
2. The pupil acquires the knowledge of photosynthetic pigments.
3. The pupil analyses the different types of photosynthetic pigment.
4. The pupil generalizes the importance of Chlorophylla molecule.
5. The pupil evaluates the importance of Chlorophylla molecule in the process
of photosynthesis.
Anticipatory set or
Introduction
In the earlier lesson you have seen about the
photosynthetic unit. Here in this chapter learn about the
pigment molecule in the photosynthetic unit. Here the
importance of Chlorophylla molecule is given with
explanation. Learn about the universal photosynthetic
unit.
Input or Procedure
Teacher presents the lesson in different steps:
(1). Small steps with clear definitions.
(2). Small questions are given for checking
understanding.
(3). In guided practice answers for the questions in the
previous phase is give with explanation.
(4). Independent practice is given with the closure of the
lesson.
Modelling
For photosynthesis light energy should be absorbed by
some pigments. Here a pigment is a molecule that
absorbs light of specific wave length in the visible
spectrum.
You can learn about the visible spectrum in the coming
lesson. The photosynthetic pigments in higher plants are
divided into Chlorophylls and Carotenoids.
In this chapter we can deal with Chlorophylls. The
chlorophylls are green pigments. Many type of
chlorophylls are known, they are Chlorophylla,
Chlorophyllb, Chlorophyllc, Chlorophylld, Chlorophylle,
bacteriochlorophyll and bacterioviridin.
Of these Chlorophylla is widely distributed in all autotropic
plants. The photochemical reaction of photosynthesis
takes place only in Chlorophylla . The term Chlorophyll
was given by Pelletier and Cavento.
Chlorophylla molecules are of different types. Some of
them are Chlorophylla673, Chlorophylla683, P680, P700
etc.
Chlorophylla is found in all photosynthetic organisms
except bacteria. Hence Chlorophylla is known as
universal photosynthetic pigment.
Chlorophyll molecules absorb only red, blue and violet
light in the visible spectrum. Chlorophyll molecules
reflects green light in the visible spectrum. So Chlorophyll
appears green. So the leaves also appear green in colour.
The maximum rate of photosynthesis has been observed
in red light. In a photosynthetic unit all pigment
molecules absorbs light energy and they transmit it to the
neighboring molecule, finally it is focused to Chlorophylla.
The photochemical reaction takes place only in
Chlorophylla.
Checking for understanding
1. ____________ is a molecule that absorbs light of
Specific wave length in the visible spectrum.
(Carbondioxide, Oxygen, Pigment)
2. The photochemical reaction takes place only in
_____________.
(Chlorophyllb. Chlorophylla, Carotenoids.)
3. Chlorophylla reflects ____________ light in the visible
spectrum.
(Red, Green, Violet)
4. Maximum rate of photosynthesis has been observed in
__________ light.
(Green, Blue, Red)
5. The term chlorophyll was given by ___________.
(Willstatler, Cavento, Stoll)
Guided Practice
1. Pigment is a molecule that absorbs light of specific
wave length in the visible spectrum.
2. The photochemical reaction takes place only in
Chlorophylla.
3. Chlorophylla reflects green light in the visible
spectrum. Red and Violet light are absorbed by
Chlorophylla molecule.
4. Maximum rate of photosynthesis takes place in red
light. Green light is reflected by Chlorophylla
molecule. Blue light is absorbed by Chlorophylla
molecule. But maximum rate of photosynthesis
takes place in red light.
5. The term chlorophyll was given by Cavento
Willstatler and Stoll found out the structure of
Chlorophyll molecule.
Answer the following questions by yourself. If you
want to check whether it is correct or wrong, you can go to another file and see whether your answers are correct.
Independent 1. Define pigment. practice
2. Name the different types of photosynthetic
pigments? And
Closure of the Lesson 3. Name the different types of chlorophyll pigments?
is considered as the universal 4. Why Chlorophylla
Photosynthetic pigment?
5. Why the leaves appear green in colour?
6. Does all the pigment molecules absorbs light energy
in the photosynthetic unit.
CORRECT ANSWER FOR LESSON – 4
1. A pigment is a molecule that absorbs light of a specific wave length in the
visible spectrum.
2. The different types of photosynthetic pigments are chlorophylls and
carotenoids.
3. The different types of chlorophyll pigments are Chlorophylla, Chlorophyllb,
Chlorophyllc, Chlorophylld, Chlorophylle, bacteriochlorophyll and
bacterioviridin.
4. Chlorophylla is found in all photosynthetic organisms except bacteria.
Hence chlorophyll is known as universal photosynthetic pigment.
5. Chlorophyll molecules reflects green light in the visible spectrum. So
Chlorophyll appears green. This is why leaves also appear green.
6. All the pigment molecules absorbs light energy and they transmit to the
neighbouring molecule, finally it is focused to Chlorophylla.
LESSON - 5
STRUCTURE OF CHLOROPHYLLa AND
CHLOROPHYLLb MOLECULE The objectives of the lesson:
1. The pupil acquires the knowledge about Chlorophylla and Chlorophyllb
molecules.
2. The pupil understands the differences between Chlorophylla and
Chlorophyllb molecules.
3. The pupil develops the skill of observing, describing and classifying the
difference between the molecules.
4. The pupil develops the elements of creativity such as visualizing and
combining the facts in a lateral thinking.
5. The pupil develops proper attitude.
Anticipatory set or
Introduction
Teacher presents the topic with the help of a diagram. By
showing the molecular structure of Chlorophylla and
Chlorophyllb students tries to differentiate the two
molecules. Teacher presents the topic with explanation.
Teacher presents the topic:
(1). With the help of molecular structure of Chlorophylla
and Chlorophyllb molecules.
(2). Teacher tries to check the understanding of students
with the help of multiple choice questions.
(3). Guided practice is given.
Input or
Procedure
(4). In independent practice small questions are given.
The Students should try to answer the questions by
themselves.
(5). If the students want to check the answers , correct
answers are give in another file.
The structure of Chlorophyll was first studied by
Willstatler, Stoll and Fisher in 1912. The basic
structure of all Chlorophyll molecules is……
Modelling
Here if we analyse the structure , all Chlorophyll
molecules is a porphyrin skeleton in which 4 pyrrole
(tetrapyrrole) rings linked together by methane groups
form a ring system. The centre of tetrapyrole is occupied
by a central core of magnesium (Mg2+). 2 covalent and 2
Co-ordinate bonds bind the Mg atom with the pyrrole
rings.
A lengthy hydrocarbon tail or side chain is attached to the
porphyrin ring. It is called the phytol. So Chlorophyll
has tadpole like structure. The phytol tail is composed of
insoluble carbon and hydrogen atoms. This tail helps to
fix the Chlorophyll molecule with the thylakoids in the
Chloroplast. Porphyrin head is hydrophilic ie; water loving
and phytol tail is lipophilic ie; fat loving.
Chlorophylla and Chlorophyllb structurally differ in the
nature of groups attached. In Chlorophylla a methyl
group ( -CH3 ) and in Chlorophyllb an aldehyde group
( -CHO ) are attached to the 3rd carbon of the 2nd pyrrole
ring in the prophyrin head. The CHO group in
Chlorophyllb increases the blue light absorption and
decreases the red light absorption peak.
Here see the difference between Chlorophylla and
Chlorophyllb molecules.
Difference between the two molecules is in the carbon
group, ie; attached to the 3rd carbon of the 2nd pyrrole
ring in the porphyrin head. Because of this difference the
empirical formula also changes.
The empirical formula of Chlorophylla is C55H72O5N4Mg
The empirical formula of Chlorophyllb is C55H70O6N4Mg
Chlorophylla is blue green where as Chlorophyllb is olive
green. Both are insoluble in organic solvents like alcohol,
acetone etc. Molecular weight of Chlorophylla is 873 and
Chlorophyllb is 907. Chlorophylla is more soluble in
petroleum ether but Chlorophyllb is more soluble in methyl
alcohol.
Checking for understanding
1. The centre of tetrapyrrole is occupied by a central
core of __________.
(Mg, Mn,C)
2. A lengthy hydrocarbon tail or side chain attached
to the prophyrin ring is called ________.
(Thylakoid, Phytol, Aldehyde)
3. In Chlorophylla molecule _______ is attached to the
3rd carbon of the 2nd pyrrole ring.
(Aldehyde, Methyl group, Water)
4. CHO group in Chlorophyllb increases the ________
absorption.
(Blue, Green, Red)
5. The empirical formulae of Chlorophyllb is _______.
(C55H72O5N4Mg, C55H71O6N4Mg, C55H70O6N4Mg)
6. The colour of Chlorophyllb molecule ____________.
(Blue green, Olive green, Green)
7. Chlorophylla is more soluble in ____________.
(Methyl alcohol, Petroleum ether, Acetone)
8. Molecular weight of Chlorophylla molecule is ____.
(907, 906, 873)
Guided Practice
1. The centre of tetrapyrrole is occupied by a central
core of Mg.
2. A lengthy hydrocarbon tail or side chain attached
to the prophyrin ring is called Phytol.
3. In Chlorophylla molecule methyl group is attached
to the 3rd carbon of the 2nd pyrrole ring. Aldehyde
group is attached in Chlorophyllb molecule.
4. CHO group in Chlorophyllb increases the blue light
absorption and decreases the red light absorption
peak.
5. The empirical formula for Chlorophyllb molecule is
C55H70O6N4Mg. The empirical formula for
Chlorophylla molecule is C55H72O5N4Mg.
6. The colour of Chlorophyllb molecule is Olive green.
Blue green is the colour of Chlorophylla molecule.
7. Chlorophylla is more soluble in Petroleum ether.
Chlorophyllb is more soluble in Methyl alcohol.
8. Molecular weight of Chlorophylla molecule is 873.
Molecular weight of Chlorophyllb molecule is 907.
Answer the following questions by yourself. If you want to check whether it is correct or wrong , you can go to another file and see whether your answers are correct.
1. Explain the structure of Chlorophyll molecule.
2. State the difference between Chlorophylla and
Chlorophyllb in a chart form.
3. Give the empirical formula for Chlorophylla and
ChlorophyllIndependent b molecule. practice
And
4. What do you mean by hydrophilic? Closure of the 5. Why maximum rate of photosynthesis doesn’t takes
place in ChlorophyllLesson
b molecule?
CORRECT ANSWER FOR LESSON – 5
1. The basic structure of all Chlorophyll molecules is a prophyrin skeleton in
Which 4 pyrrole (tetrapyrrole) rings linked together by methane groups
form a ring system. The centre of tetrapyrrole is occupied by a central
core of magnesium. Two covalent and two Co-ordinate bonds binds the
Mg atom with the pyrrole rings. A lengthy hydrocarbon tail or side chain
is attached to the prophyrin ring. It is called the phytol tail is composed
of insoluble carbon and hydrogen atom. This tail helps to fix the
Chlorophyll molecule with the thylakoids.
2.
Chlorophylla Chlorophyllb
It is a primary photosynthetic pigment
It is an accessory pigment.
Molecular weight is 873 Molecular weight is 907.
Blue green in pure state. Olive green in pure state.
Empirical formula is C55H72O5N4Mg. Empirical formula is C55H70O6N4Mg
It bears methyl group It bears aldehyde group
More soluble in Petroleum ether. More soluble in Methyl alcohol.
3. The empirical formula for Chlorophylla is C55H72O5N4Mg and the empirical
formula for Chlorophyllb is C55H70O6N4Mg.
4. Hydrophilic is water loving molecules.
5. Because of CHO group in Chlorophyllb molecule blue light absorption is
increased where as red light absorption is decreased. Only on the red
light absorption peak maximum rate of photosynthesis takes place.
LESSON - 6
CAROTENOIDS
The objectives of the lesson:
1. The pupil develops the knowledge of carotenoids.
2. The pupil understands the functions of carotenoids.
3. The pupil develops the skill of observation and classifying the different
types of photosynthetic pigment.
4. The pupil visualizes the functions of carotenoids.
5. The pupil evaluates the importance of carotenoids.
Anticipatory set
or Introduction
The topic carotenoids is presented with examples and
explanation. This topic is generally easy to study. So
study the lesson carefully.
Input or Procedure
The lesson is presented in different stages:
(1). The lesson is given with explanations and
examples.
(2). For checking understanding, small multiple choice
questions are given.
(3). In guided practice, answers with explanation for
previous stage is given.
(4). In independent practice, open ended questions are
given with closure of the lesson.
(5). Correct answers for the questions in independent
practice is given in another file.
Modelling
Carotenoids include the orange pigment Carotene and
the yellow pigment Xanthophyll or Carotenol.
Carotenes are named after carrot in which they are
abundant. These carotenes are responsible for the colour
of Pumpkin, Red Pepper, Tomato etc. The important
carotenes are β Carotenes.
Xanthophyll are more abundant than carotenes. The
yellow colour autumn leaves are due to the common
Xanthophyll, Lutein.
Carotenoids are present in all higher plants and in several
lower plants. Carotenoids absorb blue and green lights
in the visible spectrum.
The general formula of Carotene is C40H56.
The general formula of Xanthophyll is C40H56O2.
Phycobilins are another group of photosynthetic
pigment found in red algae and cyanobacteria (blue-green
algae)
Red algae contain red pigment, called Phycoerythrin
and Cyanobacteria contain blue pigment called
Phycocyanin. Both are photosynthetic water-soluble
pigments.
FUNCTIONS OF CAROTENOIDS ARE:
1. Carotenoid molecules absorb light energy and they
Transmit it to the neighbouring pigment molecules
in the photosynthetic unit.
2. Protect the Chlorophyll molecules from photo-
oxidation by picking up nascent O2 and converting
it into harmless molecular stage.
Checking for understanding
1. Xanthophyll comes under the group __________.
(Chlorophyll, Carotenoids, Phycobilins)
2. The general formula of carotene is ____________.
(C40H56O2, C40H56O, C40H56)
3. _______ group of photosynthetic pigment found in
Red algae and Cyanobacteria.
(Chlorophyll, Carotenoids, Phycobilins)
4. Red algae contains ____________.
(Phycoerythrin, Phycocyanin, Xanthophyll)
5. The general formula of Xanthophyll is _______.
(C40H56O2, C40H56O, C40H56)
Guided Practice
1. Xanthophyll comes under the group carotenoids.
Chlorophyll is the universal photosynthetic
pigment. Phycobilins are another group of
photosynthetic pigment found in Red algae and
Cyanobacteria.
2. The general formula of Carotene is C40H56.
3. Phycobilins are group of photosynthetic pigment
found in red algae or cyanobacteria.
4. Red algae contain Phycoerythrin. Phycocyanin is
seen in blue green algae or cyanobacteria.
5. The general formula for Xanthophyll is C40H56O . 2
Answer the following questions by yourself. If you
want to check whether it is correct or wrong, you can go to another file and see whether your answers are correct.
1. What are carotenoids? 2. Which pigment is responsible for the yellow color of
autumn leaves?
Independent 3. What are the functions of Carotenoids? practice And 4. What are Phycobilins?
Closure of the 5. Why Carotenoids appears in orange and yellow
colour other than green colour? Lesson
6. Which pigment is responsible for the colour of
Pumpkin, Tomato etc?
CORRECT ANSWER FOR LESSON – 6
1. Carotenoids are photosynthetic pigments. Carotenoids include the orange
pigment, carotene and the yellow pigment Xanthophyll
2. Lutein, a common Xanthophyll pigment is responsible for the yellow colour
Of autumn leaves.
3. (a) Carotenoids molecules absorb light energy and they transmit it to the
neighbouring pigment molecules.
(b) Protect the chlorophyll molecules from photo-oxidation by picking up
nascent O2 and converting it into harmless molecular stage.
4. Phycobilins are another group of photosynthetic pigments found in red
algae and cyanobacteria.
5. Carotenoids doesn’t appear in green colour because Carotenoids absorbs
green and blue light in the visible spectrum. Orange and yellow light are
reflected. So Carotenoids appear orange and yellow in colour.
6. Carotenes are responsible for the color of Pumpkin and Tomato.
LESSON - 7
PHOTOSYNTHETIC UNIT
The objectives of the lesson:
1. The pupil acquires the knowledge of photosynthetic unit.
2. The pupil understands the importance of photosynthetic unit.
3. The pupil analyses the functions of photosynthetic unit.
4. The pupil visualizes the importance of Chlorophylla in the photochemical
reaction.
5. The pupil organizes and evaluates the importance of chlorophylla
molecules in the process of photosynthesis.
Anticipatory set
or Introduction
Teacher presents the lesson in small steps so that the
students can understand the significance of
photosynthetic unit in the process of photochemical
reaction.
Input or Procedure
The lesson is presented in different stages:
(1). The lesson photosynthetic unit is given in small
steps with maximum explanation.
(2). For checking understanding, small multiple choice
questions are given.
(3). In guided practice, the correct answers for the
questions in the previous stage is given with
explanation.
(4). Independent practice is provide with the closure of
the lesson. Here open ended questions are given.
(5). Correct answers for the questions in independent
practice is given in another file. If the students are
interested they can check for correct answers.
Modelling
The smallest group of pigment molecules and enzymes,
participating in a photochemical reaction is called
Photosynthetic unit or PSU or quantasomes.
It was first isolated by Park and Biggins in 1964. Each
photosynthetic unit has a Reaction Centre and a light
harvesting pigment molecule.
First we can see about the Reaction Centre. Reaction
centre consist of a special Chlorophylla molecule which
absorbs long wave light energy. It is also called Photo
centre or trap centre.
There are 3 different types of reaction centre. They are
P700, P680, P870.
P700 – means Chlorophylla molecules with an absorption
peak around 700nm.
P680 – means Chlorophylla molecule with an absorption
peak around 680nm.
P870 – means bacteriochlorophyll molecule with an
absorption peak around 870nm.
The Reaction centre is surrounded by a number of Light
harvesting pigment molecules. The Photo centre requires
the help of Light harvesting pigment molecules in the
absorption of light energy. Second is the Light
harvesting pigment molecule.
These are those pigment molecules which absorb light
energy of different wave lengths and transfer it to the
neighbouring pigment molecules. Finally the light energy
is focused on to the Reaction Centre. The Photochemical
reaction takes place only in the Reaction Centre.
The Light harvesting pigment molecule is of two types.
They are Core Molecules and Antenna Molecules.
Core Molecules: They are pigment molecules which lie
around the reaction centre. The number of core molecule
is small, about 20-30. They are usually Chlorophylla
molecule.
Antenna Molecules : They are pigment molecules
which lie around the core molecules. The antenna
molecules are usually 200-300 in number. These
molecules include various Chlorophyll molecules and
carotenoid molecules. They absorb light energy of
different wave lengths but shorter than that of Photo
centre or Reaction centre.
The chloroplast pigment can be extracted and separated
by Paper Chromatography.
Checking for understanding
1. Photosynthetic unit was isolated by __________.
(Pelletier and Cavento, Willstatler and Fisher,
Park and Biggins)
2. The Chlorophylla molecules which absorbs long
wave light energy is called ____________.
(Light harvesting molecules, Photo centre, Stroma
thylakoid of Fret)
3. _______ is a bacteriochlorophyll.
(P700, P680, P870)
4. The Photochemical reaction takes place only in the
____________.
(Light harvesting molecules, Photo centre,
Periplastidial)
5. The pigment molecules which lie around the
reaction centre is _______.
(Antenna molecule, Core molecule, Trap centre)
1. Photosynthetic unit was isolated by Park and
Biggins. Pelletier and Cavento coined the term
chlorophyll. Willstatler Fisher and Stoll studied
the structure of chlorophyll.
2. The chlorophylla molecule which absorbs long wave
Guided Practice
light energy is called Photo centre. Light
harvesting molecules also absorbs light energy
of different wave length lesser than reaction
centre. Stroma thylakoid is seen in chloroplast.
It is the interconnection between the adjacent
grana.
3. P870 is a bacteriochlorophyll. P700 is chlorophylla
molecule with an absorption peak around 700nm.
P680 is also a chlorophylla molecule with an
absorption peak 680nm.
4. The Photochemical reaction takes place only in the
Photo centre. Photo centre or Trap centre or
Reaction centre is the only molecule which absorbs
light energy of long wave length.
5. The pigment molecules which lies around the
reaction centre is Core molecule. Antenna
molecules are pigment molecules which lie around
the core molecule. Trap centre is the another
name for Reaction centre or Photo centre.
Answer the following questions by yourself. If you want to check whether it is correct or wrong , you can go to another file and see whether your answers are correct.
1. Differentiate between Reaction centre and Light
harvesting pigment molecule.
2. Why the Photochemical reaction takes place only in
the reaction centre? Independent 3. What are the different types of Light harvesting
pigment molecules? practice
And Closure of the 4. What is the function of Light harvesting pigment
molecule? Lesson
5. Differentiate between Core molecule and Antenna
molecule.
CORRECT ANSWER FOR LESSON – 7
1. Reaction centre is a special chlorophylla molecules which absorbs long
wave light energy.
a. Photochemical reaction takes place only in the Reaction centre.
Light harvesting pigment molecules are those pigment molecules which
absorbs light energy of different wave lengths and transfer it to the
neighbouring pigment molecules and finally to the Reaction centre.
b. Photochemical reaction doesn’t takes place in light harvesting pigment
molecule.
2. For Photochemical reaction to takes place light energy of high wave length
is required and the light energy of high wave length is only absorbed by
the reaction centre. So photochemical reaction takes place only in the
reaction centre.
3. The different types of Light harvesting pigment molecules are Core
molecule and Antenna molecules.
4. The function of Light harvesting pigment molecule is to absorb light
energy of different wave lengths shorter than that of reaction centre and
transferring it to neighbouring pigment molecule are finally to the reaction
centre.
5. Core Molecules:
a. Core molecules are the molecules which lie around the Reaction centre.
b. The number of core molecule is small ie; 20 – 30.
c. Core molecules are usually chlorophylla molecules.
Antenna Molecules:
a. Antenna molecules are the molecules which lie around the core
molecule.
b. Number of antenna molecule is large ie; 200 – 300.
c. It is usually various Chlorophyll molecules and carotenoid molecules.
LESSON - 8
NATURE OF LIGHT
The objectives of the lesson:
1. The pupil acquires the knowledge about the Nature of Light.
2. The pupil tries to understand about the solar spectrum.
3. The pupil analyses the importance of visible spectrum in the importance of
Photosynthesis.
4. The pupil organizes and classifies the new knowledge.
5. The pupil evaluates the function of Solar spectrum.
Anticipatory set or
Introduction
Teacher explains the nature of light with the help of a
diagram. The content is presented in simple language
and in small frames. Read the content clearly, try to
understand, go through the questions and answers till
you are confident.
Input or Procedure
The lesson is presented in different stages:
(1). The content with the help of diagram.
(2). Small multiple choice questions are give for
understanding.
(3). Guided practice is given in the model of correct
answers for the questions in the previous stage.
(4). Independent practice is given with the closure of
the lesson.
(5). Correct answers for the questions in the independent
practice is given in another file.
Sun is the ultimate source of all radiations. All the
radiation emitted by sun constitute the Solar spectrum.
The radiation from sun is electromagnetic in character.
So these radiations are called electromagnetic radiation
and the spectrum electromagnetic spectrum. The Solar spectrum consists of different types of
radiations with different wave length.
SOLAR SPECTRUM
Modelling
From the diagram you can understand that wave length
of visible spectrum is 390nm to 760nm. This visible part
in the solar spectrum is known as Visible Spectrum.
A beam of visible spectrum transmitted through a prism
consists of seven colours called VIBGYOR ie; Violet,
Indigo, Blue, Green, Yellow, Orange and Red.
Each colour in the VIBGYOR has a different range of
wavelength. Violet has the lowest wave length and
the Red has the highest wave length in the Visible
spectrum. From the diagram, try to understand the wave
length of each colour in the Visible spectrum.
The radiation with wave length above 760nm includes
infra red radiation and radio waves. The radiation with
wave length below 390nm includes Ultra-Violet
radiation, X-Rays, Gamma Rays and Cosmic Rays.
Photosynthetic pigment molecules absorb the light rays
with wave length 400nm to 700nm in the visible
spectrum. The portion of the Visible spectrum of light
between 400nm to 700nm is called Photosynthetically
Active Radiation (PAR).
The light rays (visible rays) travel as streams of minute
particles. Each light particle is called Photon. The
energy contained in an individual photon is said to be
Quantum.
When visible light falls on the pigment molecule only
certain wave length are absorbed by the pigment
molecule, the rest is reflected. The absorbed wave length
are to specific that they bring about certain Photochemical
reactions.
Checking for understanding
1. All radiations emitted by sun constitute the
__________.
(Solar spectrum, Ultra violet spectrum, Infrared
spectrum)
2. The wave length of visible spectrum ranges from
____________.
(10nm to 390nm, 390nm to 760nm, 760nm to
1,00,000nm)
3. The portion of the Visible spectrum of light
between 400nm and 700nm is called _________.
(Photo centre, Photosynthetic unit,
Photosynthetically active radiation)
4. Each light particle is called ____________.
(Proton, Neutron, Photon)
5. The energy contained in an individual photon is
called ___________.
(Neutron, Quantum, Electron)
Guided Practice
1. All radiation emitted by sun constitute the Solar
spectrum.
2. The wave length of visible spectrum ranges from
390nm to 760nm. 10nm to 390nm is Ultra violet
rays. 760nm to 1,00,000nm is Infra red rays.
3. The portion of the visible spectrum of light
between 400nm to 700nm is called
Photsynthetically active radiation. Photo centre is
the reaction centre ie; a Chlorophylla molecule
which absorbs long wave light energy. Photo
synthetic unit is the smallest group of pigment
molecules, participating in a Photochemical
reaction.
4. Each light particle is called Photon.
5. The energy contained in an individual photon is
called Quantum.
Answer the following questions by yourself. If you want to check whether it is correct or wrong , you can go to another file and see whether your answers are correct.
1. Why Solar spectrum is known as electromagnetic
spectrum?
2. What is visible spectrum?
3. Which all colours are there in visible spectrum.
Explain with there wave lengths.
Independent practice
And
4. What are Photosynthetically active radiation? Closure of the
Lesson 5. Define Photon.
6. Define Quantum.
CORRECT ANSWER FOR LESSON – 8
1. The radiations in the Solar spectrum is electro magnetic in character.
Hence the Solar spectrum is called Electro magnetic spectrum.
2. The radiation which ranges from 390nm to 700nm is called Visible
spectrum.
3. The colours in the Visible spectrum is VIBGYOR ie; Violet, Indigo, Blue,
Green, Yellow, Orange and Red. The wave length of
Violet - 390nm to 430nm
Indigo - 430nm to 470nm
Blue - 470nm to 500nm
Green - 500nm to 560nm
Yellow - 560nm to 600nm
Orange - 600nm to 650nm
Red - 650nm to 760nm
4. Photosynthetic pigment molecules absorb the light rays with wave length
400nm to 700nm. The portion of visible spectrum of light between
400nm to 700nm is called Photosynthetically Active Radiation (PAR).
5. Each light particle is known as Photon.
6. The energy contained in a individual photon is called Quantum.
LESSON - 9
ABSORPTION AND ACTION SPECTRUM
The objectives of the lesson:
1. The pupil acquires the knowledge of Absorption and action spectrum.
2. The pupil understands the concept.
3. The pupil analyses the importance of Absorption and Action spectrum.
4. The pupil organizes, evaluates and thus classifies the difference between
Absorption and Action spectrum
Anticipatory set or
Introduction
The teacher explains the concept of Absorption and
Action spectrum with the help of the diagram. The
concept is explained in simple language and for further
clarification the graphical representation of Absorption
and Action spectrum are given.
Input or Procedure
The lesson is prepared in different steps:
(1). The concept is given in simple language. For clear
Understanding graphical representation of
Absorption and Action spectrum is given.
(2). For checking understanding small multiple choice
questions are given.
(3). In guided practice the correct answer for the
questions in the previous step is given with
explanation.
(4). Independent practice and closure of the lesson is
given with small open ended questions.
(5). The correct answers for the questions in the
independent practice is given in another file.
From the previous lesson you have learnt that Chloroplast
pigments are responsible for the Absorption of light. A
particular pigment absorbs light rays with different wave
length. For example; Chlorophylla absorbs blue and red
lights in the visible spectrum. The amount of light
absorbed by a pigment can be plotted.
Modelling
The graphical representation of the absorption maxima
against wave length is called Absorption spectrum.
Action spectrum
Photosynthesis takes place in visible light ranging from
400nm to 700nm wave length. It does not take place at
the same rate with other wave length.
Therefore, the rate of Photosynthesis at different wave
length is measured and plotted by amount of Action ie; O2
evolved and Co2 utilisation etc. This graphical
representation of rate of Photosynthesis against the wave
length is called Action spectrum.
In 1882, T.W.Engelmann plotted the first action spectrum
of photosynthesis using green algae. The maximum
rate of Photosynthesis occurs with red light of the
visible spectrum.
Checking for understanding
1. ________ pigment is responsible for the absorption
of light.
(Chlorophyll, Xanthophyll, Carotenoid)
2. The graphic representation of the absorption
maxima against wave length is called __________.
(Action spectrum, Absorption spectrum, Visible
spectrum)
3. The graphical representation of the rate of
photosynthesis against the wave length is called
_________.
(Action spectrum, Absorption spectrum, Visible
spectrum)
4. Maximum rate of photosynthesis takes place in
_________ light.
(Blue, Red, Green)
5. The rate of photosynthesis is measured by the
amount of action of __________.
(Co2 utilisation, O2 utilisation)
Guided Practice
1. Chlorophyll pigment is responsible for the
absorption of light.
2. The graphic representation of the absorption
maxima against wave length is called Absorption
spectrum.
3. The graphic representation o rate of
photosynthesis against the wave length is called
Action spectrum.
4. Maximum rate of photosynthesis takes place in Red
light.
5. The rate of photosynthesis is measured by the
amount of action of Co2 utilisation.
Answer the following questions by yourself. If you
want to check whether it is correct or wrong , you can go to another file and see whether your answer are correct.
Independent 1. Explain Absorption spectrum with graphical
representation? practice
And Closure of the
2. What is Action spectrum? Explain with graphical
represention.
Lesson
3. How does the rate of photosynthesis measured?
4. Why does maximum rate of photosynthesis takes
place in red light?
CORRECT ANSWER FOR LESSON – 9
1. The graphical representation of the absorption maxima against wave
length is called Absorption spectrum. The percentage of absorption is
plotted on the Y axis and wave length is plotted on the X axis.
2. The graphical representation of rate of photosynthesis against the wave
length is called Action spectrum.
3. The rate of photosynthesis is measured by the amount of O2 evolved and
Co2 utilisation.
4. Maximum rate of photosynthesis takes place at maximum wave length.
Red light has maximum wave length so maximum rate of photosynthesis
takes place at red light.
APPENDIX – II
LESSON TRANSCRIPTS FOR E-MAIL LEARNING
LESSON TRANSCRIPT 1
PHOTOSYNTHESIS
Author: Mary Sindhu J School:
Subject: Biology Standard:
Autotrophic plants prepare their own food material through the process
called Photosynthesis. Photosynthesis is the process of synthesis of
carbohydrates from carbondioxide and water in the presence of sunlight and
chlorophyll.
It is the most important synthetic process on earth. Here the solar energy
is fixed in the form of chemical energy ie; the plants have the capacity of
capturing light energy from the sun and converting it into chemical energy.
Here the kinetic energy of sunlight is transformed into chemical energy
and stored in the carbohydrate molecules.
This chemical energy is used for the growth and development of all forms
of life in the universe.
Approximately 90% of the world’s photosynthesis is carried out by marine
algae and fresh water algae and all animals depend on plants and their source of
energy.
Photosynthesis is considered as the natural physio-biological process of
the world on which the existence of life in earth depends. So photosynthesis is
the single most important biological process required for human existence.
Checking for Understanding:
1. Photosynthesis is the process of synthesis of _______.
(Carbohydrate, Fat)
2. During photosynthesis ______energy is converted into _______energy.
(Light energy-Chemical energy, Sound energy-Chemical energy)
3. 90% of photosynthesis is carried out by ___________and _______________.
(Marine algae & Fresh water algae, Animals & Zooplankton)
4. The natural physio-biological process of the world is ___________.
(Photosynthesis, Respiration)
LESSON TRANSCRIPT 2
CHLOROPLAST – THE SITE FOR PHOTOSYNTHESIS
Author: Mary Sindhu J School:
Subject: Biology Standard:
Photosynthesis is the process of synthesis of carbohydrate from Co2 and
water in the presence of sunlight and chlorophyll.
6CO2 + 12H2O C6H12O6 + 6H2O + 6O2
Here the photosynthesis takes place in the green parts of the plant body
such as leaves, green stems and in green floral parts etc. In leaves the
photosynthesis takes place in the mesophyll tissues.
Structure of Leaf
In the structure of a leaf the green tissues seen in between the upper and
lower epidermis is the mesophyll tissue. The mesophyll is differentiated
into palisade parenchyma and spongy parenchyma. Both these parenchyma
cells contain the green plastid-CHLOROPLAST.
These chloroplasts are the characteristic structure of green plants. The
chloroplasts are green due to the presence of green pigment chlorophyll. Here
the chloroplasts are considered as the seats of photosynthesis, they synthesis
starch by using CO2 and sunlight. So chloroplast is considered as the
photosynthetic apparatus.
The chloroplasts are distributed homogeneously in the cytoplasm of plant
cell. Their diameter is about 5 to 8 µ. The size of the chloroplast varies from
species to species. In green algae, the chloroplast has various shapes, example.
Ribbon shaped (Spirogyra)
Star shaped (Zygnema)
Girdle shaped (Ulothrix)
Disc shaped (Caulerpa)
Reticulate shaped (Oedogonium)
Cup shaped (Chlamydomanas)
Checking for understanding:
1. Photosynthesis takes place only in _____________.
(Green parts, Flowers, Roots)
2. The green plastids seen in the cytoplasm are called _________________.
(Chloroplast, Palisade tissue, Vacuoles)
3. ____________ is considered as the photosynthetic apparatus.
(Mesophyll tissue, Flowers, Chloroplast)
4. The diameter of the chloroplast varies from ________.
(5-10 µ, 5-8 µ, 5-6 µ)
5. The shape of chloroplast in chlamydomonas is ______________.
(Star shaped, Girdle shaped, Cup shaped)
LESSON TRANSCRIPT 3
STRUCTURE OF CHLOROPLAST
Author: Mary Sindhu J School:
Subject: Biology Standard:
Now we can see the structure of chloroplast.
Structure of Chloroplast
The chloroplast is surrounded by two unit membrane. Each membrane is
lipo-proteinaceous in nature. The space in between two membranes is known as
periplastidial space. The space inside the chloroplast is filled with a
proteinaceous fluid called Stroma. The stroma contains DNA, Ribosome’s and
Enzymes for carbon assimilation. The enzymes found in stroma are capable of
utilizing ATP and NADPH2 to produce carbohydrates.
A number of membrane bound, sac like structure are embedded in the
stroma. The structures are called the Thylakoids or Lamellae. The thylakoids
are seen in groups. Each group is known as Granum. Each thylakoid in a
granum is called the grana thylakoid.
The thylakoid which interconnects the adjacent grana is called the
stromathylakoid or fret or intergranal thylakoid.
Each thylakoid is also a double membrane-bound structure. The green
pigment molecules, chlorophyll are seen as units inside the thylakoids. In the
process of photosynthesis there are two types of reaction ie; Light reaction or
Photochemical reaction and Dark reaction or Biosynthetic reaction.
Each smallest unit of pigment molecules in the thylakoids which is capable
of carrying out the light reaction is said to be Photosynthetic unit or PSU or
Quantasomes.
The granum is the site for photochemical reaction and the stroma is the
site for dark reaction in photosynthesis. These chloroplast are located at the
outer margins ie; periphery of the cytoplasm with their broader surfaces parallel
to the cell wall of the mesophyll cells.
Checking for understanding:
1. Chloroplast membrane is made up of _____________.
(Lipoprotein, Cellulose, Chitin)
2. The space inside the chloroplast is filled with a protienaceous fluid called
_____________.
(Grana, Stroma, Thylakoid)
3. The number of membrane bound sac like structure Embedded in the stroma
are _______________.
(Grane, Thylakoid or Lamellae, Stroma thylakoid)
4. The site for light reaction is _________________.
(Grana, Stroma)
5. The site for dark reaction is _________________.
(Grana, Stroma).
6. Each small unit of pigment molecule capable of carrying out photosynthesis
are called ________ or _________.
(Stroma or Grana, Stroma thylakoid or Grana thylakoid, Photosynthetic unit of
Quantasome)
LESSON TRANSCRIPT 4
PHOTOSYNTHETIC PIGMENT – CHLOROPHYLLa
Author: Mary Sindhu J School:
Subject: Biology Standard:
For photosynthesis light energy should be absorbed by some pigments.
Here a pigment is a molecule that absorbs light of specific wave length in the
visible spectrum.
You can learn about the visible spectrum in the coming lesson. The
photosynthetic pigments in higher plants are divided into Chlorophylls and
Carotenoids.
In this chapter we can deal with Chlorophylls. The chlorophylls are
green pigments. Many type of chlorophylls are known, they are Chlorophylla,
Chlorophyllb, Chlorophyllc, Chlorophylld, Chlorophylle, bacteriochlorophyll and
bacterioviridin. Of these Chlorophylla is widely distributed in all autotropic
plants. The photochemical reaction of photosynthesis takes place only in
Chlorophylla . The term Chlorophyll was given by Pelletier and Cavento.
Chlorophylla molecules are of different types. Some of them are
Chlorophylla673, Chlorophylla683, P680, P700 etc. Chlorophylla is found in all
photosynthetic organisms except bacteria. Hence Chlorophylla is known as
universal photosynthetic pigment.
Chlorophyll molecules absorb only red, blue and violet light in the visible
spectrum. Chlorophyll molecules reflects green light in the visible spectrum. So
Chlorophyll appears green. So the leaves also appear green in color. The
maximum rate of photosynthesis has been observed in red light. In a
photosynthetic unit all pigment molecules absorbs light energy and they transmit
it to the neighboring molecule, finally it is focused to Chlorophylla. The
photochemical reaction takes place only in Chlorophylla.
Checking for understanding:
1. ____________ is a molecule that absorbs light of Specific wave length in the
visible spectrum.
(Carbondioxide, Oxygen, Pigment)
2. The photochemical reaction takes place only in _____________.
(Chlorophyllb. Chlorophylla, Carotenoids)
3. Chlorophylla reflects ____________ light in the visible spectrum.
(Red, Green, Violet)
4. Maximum rate of photosynthesis has been observed in __________ light.
(Green, Blue, Red)
5. The term chlorophyll was give by ___________.
(Willstatler, Cavento, Stoll)
LESSON TRANSCRIPT 5
STRUCTURE OF CHLOROPHYLLa AND
CHLOROPHYLLb MOLECULE
Author: Mary Sindhu J School:
Subject: Biology Standard:
The structure of Chlorophyll was first studied by Willstatler, Stoll and
Fisher in 1912. The basic structure of all Chlorophyll molecule is……
Here if we analyse the structure , all Chlorophyll molecules is a porphyrin
skeleton in which 4 pyrrole (tetrapyrrole) rings linked together by methane
groups from a ring system. The centre of tetrapyrole is occupied by a central
core of magnesium (Mg2+). 2 covalent and 2 Co-ordinate bonds bind the Mg
atom with the pyrrole rings.
A lengthy hydrocarbon tail or side chain is attached to the porphyrin ring.
It is called the phytol. So Chlorophyll has tadpole like structure. The phytol tail
is composed of insoluble carbon and hydrogen atoms. This tail helps to fix the
Chlorophyll molecule with the thylakoids in the Chloroplast. Porphyrin head is
hydrophilic ie; water loving and phytol tail is lipophilic ie; fat loving.
Chlorophylla and Chlorophyllb structurally differ in the nature of groups
attached. In Chlorophylla a methyl group ( -CH3 ) and in Chlorophyllb an
aldehyde group ( -CHO ) are attached to the 3rd carbon of the 2nd pyrrole ring in
the prophyrin head. The CHO group in Chlorophyllb increases the blue light
absorption and decreases the red light absorption peak.
Here see the difference between Chlorophylla and Chlorophyllb molecules.
The only difference between the two molecules is in the carbon group, ie;
attached to the 3rd carbon of the 2nd pyrrole ring in the porphyrin head. Because
of this difference the empirical formula also changes.
The empirical formula of Chlorophylla is C55H72O5N4Mg
The empirical formula of Chlorophyllb is C55H70O6N4Mg
Chlorophylla is blue green where as Chlorophyllb is olive green. Both are
insoluble in organic solvents like alcohol, acetone etc. Molecular weight of
Chlorophylla is 873 and Chlorophyllb is 907. Chlorophylla is more soluble in
petroleum ether but Chlorophyllb is more soluble in methyl alcohol.
Checking for understanding:
1. The centre of tetrapyrrole is occupied by a central core of __________.
(Mg, Mn, C)
2. A lengthy hydrocarbon tail or side chain attached to the prophyrin ring is
called ________.
(Thylakoid, Phytol, Aldehyde)
3. In Chlorophylla molecule _______ is attached to the 3rd carbon of the 2nd
pyrrole ring.
(Aldehyde, Methyl group, Water)
4. CHO group in Chlorophyllb increases the ________ absorption.
(Blue, Green, Red)
5. The empirical formulae of Chlorophyllb is _______.
(C55H72O5N4Mg, C55H71O6N4Mg, C55H70O6N4Mg)
6. The colour of Chlorophyllb molecule ____________.
(Blue green, Olive green, Green)
7. Chlorophylla is more soluble in ____________.
(Methyl alcohol, Petroleum ether, Acetone)
8. Molecular weight of Chlorophylla molecule is ____.
(907, 906, 873)
LESSON TRANSCRIPT 6
CAROTENOIDS
Author: Mary Sindhu J School:
Subject: Biology Standard:
Carotenoids include the orange pigment Carotene and the yellow
pigment Xanthophyll or Carotenol.
Carotenes are named after carrot in which they are abundant. These
carotenes are responsible for the colour of Pumpkin, Red Pepper, Tomato etc.
The important carotenes are β Carotenes.
Xanthophyll are more abundant than carotenes. The yellow colour
autumn leaves are due to the common Xanthophyll, Lutein.
Carotenoids are present in all higher plants and in several lower plants.
Carotenoids absorb blue and green lights in the visible spectrum.
The general formula of Carotene is C40H56.
The general formula of Xanthophyll is C40H56O2.
Phycobilins are another group of photosynthetic pigment found in red
algae and cyanobacteria (blue-green algae)
Red algae contain red pigment, called Phycoerythrin and Cyanobacteria
contain blue pigment called Phycocyanin. Both are photosynthetic water-
soluble pigments.
Functions of Carotenoids are:
1. Carotenoid molecules absorb light energy and they transmit it to the
neighbouring pigment molecules in the photosynthetic unit.
2. Protect the Chlorophyll molecules from photo-oxidation by picking up
nascent O2 and converting it into harmless molecular stage.
Checking for understanding:
1. Xanthophyll comes under the group __________.
(Chlorophyll, Carotenoids, Phycobilins)
2. The general formula of carotene is ____________.
(C40H56O2, C40H56O, C40H56)
3. _______ group of photosynthetic pigment found in Red algae and
Cyanobacteria.
(Chlorophyll, Carotenoids, Phycobilins)
4. Red algae contain ____________.
(Phycoerythrin, Phycocyanin, Xanthophyll)
5. The general formula of Xanthophyll is _______.
(C40H56O2, C40H56O, C40H56)
LESSON TRANSCRIPT 7
PHOTOSYNTHETIC UNIT
Author: Mary Sindhu J School:
Subject: Biology Standard:
The smallest group of pigment molecules and enzymes, participating in a
photochemical reaction is called Photosynthetic unit or PSU or
quantasomes. It was first isolated by Park and Biggins in 1964. Each
photosynthetic unit has a Reaction Centre and a light harvesting pigment
molecule.
First we can see about the Reaction Centre. Reaction centre consist of
a special Chlorophylla molecule which absorbs long wave light energy. It is also
called Photo centre or trap centre.
There are 3 different types of reaction centre. They are P700, P680, P870.
P700 – means Chlorophylla molecules with an absorption peak around 700nm.
P680 – means Chlorophylla molecule with an absorption peak around 680nm.
P870 – means bacteriochlorophyll molecule with an absorption peak around
870nm.
The Reaction centre is surrounded by a number of Light harvesting
pigment molecules. The Photo centre requires the help of Light harvesting
pigment molecules in the absorption of light energy. Second is the Light
harvesting pigment molecule.
These are those pigment molecules which absorb light energy of different
wave lengths and transfer it to the neighbouring pigment molecules. Finally the
light energy is focused on to the Reaction Centre. The Photochemical reaction
takes place only in the Reaction Centre.
The Light harvesting pigment molecule is of two types. They are Core
Molecules and Antenna Molecules.
Core Molecules:
They are pigment molecules which lie around the reaction centre. The
number of core molecule is small, about 20-30. They are usually Chlorophylla
molecule.
Antenna Molecules :
They are pigment molecules which lie around the core molecules. The
antenna molecules are usually 200-300 in number. These molecules include
various Chlorophyll molecules and carotenoid molecules. They absorb light
energy of different wave lengths but shorter than that of Photo centre or
Reaction centre.
The chloroplast pigment can be extracted and separated by Paper
Chromatography.
Checking for understanding:
1. Photosynthetic unit was isolated by __________.
(Pelletier and Cavento, Willstatler and Fisher, Park and Biggins)
2. The Chlorophylla molecules which absorbs long wave light energy is called
____________.
(Light harvesting molecules, Photo centre, Stroma thylakoid of Fret)
3. _______ is a bacteriochlorophyll.
(P700, P680, P870)
4. The Photochemical reaction takes place only in the ____________.
(Light harvesting molecules, Photo centre, Periplastidial)
5. The pigment molecules which lie around the reaction centre is _______.
(Antenna molecule, Core molecule, Trap centre)
LESSON TRANSCRIPT 8
NATURE OF LIGHT
Author: Mary Sindhu J School:
Subject: Biology Standard:
Sun is the ultimate source of all radiations. All the radiation emitted by
sun constitute the Solar spectrum. The radiation from sun is electromagnetic
in character. So these radiations are called electromagnetic radiation and the
spectrum electromagnetic spectrum. The Solar spectrum consists of
different types of radiations with different wave length.
SOLAR SPECTRUM
From the diagram you can understand that wave length of visible
spectrum is 390nm to 760nm. This visible part in the solar spectrum is known as
Visible Spectrum.
A beam of visible spectrum transmitted through a prism consists of seven
colours called VIBGYOR ie; Violet, Indigo, Blue, Green, Yellow, Orange and Red.
Each colour in the VIBGYOR has a different range of wavelength.
Violet has the lowest wave length and the Red has the highest wave
length in the visible spectrum. From the diagram, try to understand the wave
length of each colour in the visible spectrum.
The radiation with wave length above 760nm includes infra red radiation
and radio waves. The radiation with wave length below 390nm includes Ultra-
Violet radiation, X-Rays, Gamma Rays and Cosmic Rays.
Photosynthetic pigment molecules absorb the light rays with wave length
400nm to 700nm in the visible spectrum. The portion of the Visible spectrum of
light between 400nm to 700nm is called Photosynthetically Active Radiation
(PAR).
The light rays (visible rays) travel as streams of minute particles. Each
light particle is called Photon. The energy contained in an individual photon is
said to be Quantum.
When visible light falls on the pigment molecule only certain wave lengths
are absorbed by the pigment molecule, the rest is reflected. The absorbed wave
lengths are to specific that they bring about certain Photochemical reactions.
Checking for understanding:
1. All radiations emitted by sun constitute the __________.
(Solar spectrum, Ultra violet spectrum, Infrared spectrum)
2. The wave length of visible spectrum ranges from ____________.
(10nm to 390nm, 390nm to 760nm, 760nm to 1,00,000nm)
3. The portion of the Visible spectrum of light between 400nm and 700nm is
called _________.
(Photo centre, Photosynthetic unit, Photosynthetically active radiation)
4. Each light particle is called ____________.
(Proton, Neutron, Photon)
5. The energy contained in an individual photon is called ___________.
(Neutron, Quantum, Electron)
LESSON TRANSCRIPT 9
ABSORPTION AND ACTION SPECTRUM
Author: Mary Sindhu J School:
Subject: Biology Standard:
From the previous lesson you have learnt that Chloroplast pigments are
responsible for the Absorption of light. A particular pigment absorbs light rays
with different wave length. For example; Chlorophylla absorbs blue and red
lights in the visible spectrum. The amount of light absorbed by a pigment can be
plotted.
The graphical representation of the absorption maxima against wave length is
called Absorption spectrum.
Action spectrum
Photosynthesis takes place in visible light ranging from 400nm to 700nm
wave length. It does not take place at the same rate with other wave length.
Therefore, the rate of Photosynthesis at different wave length is measured
and plotted by amount of Action ie; O2 evolved and Co2 utilisation etc. This
graphical representation of rate of Photosynthesis against the wave length is
called Action spectrum.
In 1882, T.W.Engelmann plotted the first action spectrum of
photosynthesis using green algae. The maximum rate of Photosynthesis
occurs with red light of the visible spectrum.
Checking for understanding:
1. ________ pigment is responsible for the absorption of light.
(Chlorophyll, Xanthophyll, Carotenoid)
2. The graphic representation of the absorption maxima against wave length
is called __________.
(Action spectrum, Absorption spectrum, Visible spectrum)
3. The graphical representation of the rate of photosynthesis against the
wave length is called _________.
(Action spectrum, Absorption spectrum, Visible spectrum)
4. Maximum rate of photosynthesis takes place in _________ light.
(Blue, Red, Green)
5. The rate of photosynthesis is measured by the amount of action of
__________.
(Co2 utilisation, O2 utilisation)
APPENDIX- III
LESSON TRANSCRIPTS FOR VOICE CHATTING TECHNIQUE
LESSON TRANSCRIPT I
PHOTOSYNTHESIS
Author : School : Subject : Standard :
Content Objective
In this lesson the significance of photosynthesis is
explained. Teacher expresses the content with the help
of examples and thought provoking questions.
(1). The pupil acquires the knowledge of photosynthesis.
Stated objective Purpose
or Goal of lesson
(2). The pupil understands the significance of
photosynthesis.
(3). The pupil analyses the process of photosynthesis as
the important biological process.
(4). The pupil organizes, evaluates and makes inferences
on the importance of photosynthesis.
Anticipatory set
or Introduction
Teacher gives the concept of photosynthesis and their
significance through clear explanation.
Input or
Procedure
The lesson is prepared in different phases:
1. Teacher presents the lesson in small steps.
2. Teacher guides the students to learn the lesson
with divergent questions.
3. Teacher explains the questions through guided
practice.
4. Students learn the lesson through thought
provoking questions.
Modelling
Teacher presents the topic photosynthesis through small
steps. Teacher expresses the content by using simple
language.
Checking for
understanding
Small multiple choice questions are asked to the
students. So that the students can identify whether they
have mastered the content.
Guided Practice
In guided practice the answers for the questions in the
previous step is explained. If the students wish to hear
the content once again the teacher explains the content
once again in the phase.
Independent practice
And Closure of the
Lesson
In Independent practice small open ended questions are asked. Students should answer the questions without choice. The questions are:
1. Why photosynthesis is considered as a significant
process?
2. What is photosynthesis?
3. During photosynthesis which energy is converted
into chemical energy?
4. Which is the most important biological process
required for human existence?
LESSON TRANSCRIPT 2
CHLOROPLAST – THE SITE FOR PHOTOSYNTHESIS
Author : School : Subject : Standard :
Content Objective
The students after learning the concept photosynthesis
the concept chloroplast are explained. The importance of
chloroplast and why chloroplast is considered as the
photosynthetic apparatus is explained in this lesson.
Stated objective
Purpose or Goal of lesson
(1). The pupil understands the importance of chloroplast
in the process of photosynthesis.
(2). The pupil identifies the structure of leaf and its
importance.
(3). The pupil analyses the importance of photosynthetic
apparatus.
(4). The pupil differentiates the different shapes of
chloroplast.
(5). The pupil synthesis the knowledge that size and
shape of chloroplast varies from species to species.
Anticipatory set or
Introduction
Teacher explains the position of chloroplast with the help
of a diagram The structure of Leaf. Teacher explains the
significance of chloroplast and why chloroplast is said to
be the photosynthetic apparatus.
Input or Procedure
The lesson is prepared in different steps:
1. Teacher explains the content in series of small
steps.
2. Teacher checks the understanding of students with
multiple choice questions.
3. Teacher explains the content once again in the
guided practice.
4. Teacher makes the closure of lesson through
Independent practice.
Modelling
Teacher explains where the chloroplast is present with the
help of the diagram of structure of Leaf. Teacher explains
why chloroplast is considered as the photosynthetic
apparatus and explains that size and shape of chloroplast
varies from species to species. To show various shape of
chloroplast the teacher with the help of diagrams site the
shape of chloroplast of some green algae.
Checking for
understanding
Small multiple choice questions are asked to the
students. So that the students can identify whether they
have mastered the content.
Guided Practice
Here the teacher explains the answers for the questions
in the previous step. Once again the teacher explains the
content for the students through this page.
In Independent practice the teacher ask small open
ended questions such as Independent
practice 1. Why chloroplast is considered as the photosynthetic
apparatus? And Closure of the
Lesson 2. Why photosynthesis takes place in the green parts
of the plant body?
3. Why photosynthesis doesn’t takes place in the roots
of the plants?
LESSON TRANSCRIPT 3
STRUCTURE OF CHLOROPLAST
Author : School : Subject : Standard :
Content Objective
The pupil tries to understand the structure of Chloroplast.
Students perceives the terms such as periplastidial space,
stroma, grana, thylakoid, frets, photosynthetic unit or
quantasomes.
The pupil understands the structure of chloroplast.
Stated objective Purpose
(1) The pupil explains the importance of chloroplast.
(2) The pupil analyses the role of chloroplast in the or Goal of lesson process of photosynthesis.
(3) The pupil syntheses the knowledge about the function
of chloroplast.
The teacher presents the topic with the help of the
diagram of chloroplast. The teacher explains the concept
in simple language.
Anticipatory set or
Introduction
Input or Procedure
The lesson is prepared in different steps :
(1) The lesson is presented with the help of a
diagram.
(2) Small questions are given for checking
understanding.
(3) Answers for the questions in the previous step are
given.
(4) Independent practice with the closure of the
lesson is given.
Modelling
The teacher explains various terms which is involved in
the structure of chloroplast with the help of diagram.
Teacher interacts with the students and through
discussion the students tries to learn about photosynthetic
unit or quantasomes, stroma, grana, and fret.
Checking for understanding
After explaining the structure of chloroplast for checking
the students understanding teacher ask multiple choice
questions.
Guided Practice
In guided practice if the students are unable to answer
the questions in the previous stage the teacher explains
the concept once again for the students in the question
answer form.
Independent practice
And Closure of the
Lesson
In Independent practice small open ended questions are asked. Students should answer the questions without choice. The questions are:
1. Describe the structure of Chloroplast?
2. What are quatasomes?
3. Why light reaction takes place only in granum of
chloroplast?
4. Why chloroplasts are located on the periphery of
cytoplasm?
5. Define fret?
LESSON TRANSCRIPT 4
PHOTOSYNTHETIC PIGMENT - CHLOROPHYLLa
Author : School : Subject : Standard :
The student should understand the importance of
pigments in the process of photosynthesis. Moreover
students should generalize the importance of chlorophylla
molecule and why chlorophylla is considered as the
Universal photosynthetic pigment.
Content Objective
Stated objective
Purpose or Goal of lesson
(1). The pupil tries to understand the importance of
pigments in photosynthesis.
(2). The pupil acquires the knowledge of photosynthetic
pigment.
(3). The pupil analyses the different types of
photosynthetic pigments.
(4). The pupil generalizes the importance of chlorophylla
molecule.
(5). The pupil evaluates the importance of chlorophylla
molecule in the process of photosynthesis.
Anticipatory set or
Introduction
Here the teacher explains the content before the students
with examples so that the students would understand the
content thoroughly.
The lesson is prepared in different phases: 1. Teacher presents the lesson in small steps with
Input or
Procedure
clear definitions.
2. Teacher guides the students to learn the lesson
with divergent questions. 3. Teacher explains the questions through guided
practice. 4. Students learn the lesson through thought
provoking questions.
The teacher presents the concept with clear definitions.
Through discussion the new terms such as Universal
photosynthetic pigment and visible spectrum are
introduced before the students. Teacher with explanations
presents the topic.
Modelling
The teacher after explaining the concept asks question to
check the understanding of the students. Small multiple
choice questions are asked so that if the students had
understood the concept can answer easily.
Checking for understanding
After asking questions if the students didn’t answer the
questions the teacher explains the answers with suitable
examples. Here in guided practice the teacher explains
the concept once again for the students.
Guided Practice
In Independent practice small open ended questions are asked. If the students have analyzed the concept the students will be able to answer the questions. The questions are:
Independent 1. What is a pigment? practice
2. Name the different type of photosynthetic
pigments. And
Closure of the Lesson 3. Name the different types of photosynthetic
Chlorophyll pigments.
4. Why chlorophylla is considered as the universal
photosynthetic pigment?
5. Why the leaves appear green in colour?
6. Does all the pigment molecules appear light energy
in the photosynthetic unit?
LESSON TRANSCRIPT 5
STRUCTURE OF CHLOROPHYLLa AND CHLOROPHYLLb
MOLECULE.
Author : School : Subject : Standard :
Content Objective
Teacher introduces the molecular structure of
chlorophylla and chlorophyllb molecule with the help of
the diagrams. The students should familiarize the terms
such as tetrapyrrole, phytol, hydrophilic, lipophilic etc.
The students should categorise the difference between
chlorophylla and Chlorophyllb molecule.
(1). The pupil acquires the knowledge about Chlorophylla
Stated objective
Purpose or Goal of lesson
and Chlorophyllb molecules.
(2). The pupil understands the differences between
Chlorophylla and Chlorophyllb molecules.
(3). The pupil develops the skill of observing, describing
and classifying the difference between the
molecules.
(4). The pupil develops the elements of creativity such as
visualizing and combining the facts in a lateral
thinking.
(5). The pupil develops proper attitude.
Anticipatory set
or Introduction
Teacher explains the general structure of Chlorophyll
molecule with the help of the diagram of the molecular
structure of Chlorophyll molecule. After that the teacher
presents the diagram of the molecular structure of
Chlorophylla and Chlorophyllb molecule from the diagrams
the students should try to differentiate the two molecules.
The lesson is prepared in different steps: 1. Teacher explains the content with the help of
molecular structure of Chlorophyll and Chlorophylla b
molecules.
Input or Procedure 2. Teacher checks the understanding of students with
multiple choice questions.
3. Teacher explains the content once again in the
guided practice.
4. Teacher makes the closure of lesson through
Independent practice.
First the teacher explains the structure of Chlorophyll
molecule. Than by showing the molecular structure of the
Chlorophyll molecule the teacher explains the terms such
as tetrapyrrole and phytol tail. After that the teacher
shows the diagram of the molecular structure of
Chlorophyll
Modelling and Chlorophylla b molecule. The students by
observing the diagrams should give the differences
between the two molecule. The teacher gives the
empirical formulae of Chlorophyll
and Chlorophylla b
molecule.
Checking for For checking the understanding of the students the understanding
teacher asks multiple choice questions.
After asking the questions the teacher would explain the
content once again for the students for thorough
understanding. Than the teacher explains the answers for
all questions which was asked.
Guided practice
After mastering the content thoroughly through guided
practice the teacher asks small open ended questions like,
Independent (1) Explain the structure of Chlorophyll molecule? practice And (2) State the difference between Chlorophylla and
ChlorophyllClosure of the molecule. bLesson (3) What do you mean by hydrophilic?
(4) Why maximum rate of photosynthesis doesn’t
takes place in Chlorophyllb molecule?
LESSON TRANSCRIPT 6
CAROTENOIDS
Author : School : Subject : Standard :
Content Objective
The students should understand what are carotenoids and
what are the different types of carotenoids? Why carrot
has orange colour? Why during autumn the leaves
become yellow in colour? The student tries to learn the
general formulae of Carotenoid and Xanthophyll.
(1) The pupil develops the knowledge of Carotenoids.
(2) The pupil understands the functions of
Carotenoids.
Stated objective
Purpose (3) The pupil develops the skill of observation and
classifying the different types of photosynthetic
pigment.
or Goal of lesson
(4) The pupil develops visualizes the functions of
Carotenoids.
(5) The pupil evaluates the importance of Carotenoids.
The topic Carotenoid is generally easy for the students.
The teacher presents the topic with examples and
explanations.
Anticipatory set or
Introduction
(1) The teacher presents the topic with examples and
explanation.
Input or (2) Teacher asks certain divergent questions for
checking the understanding of the students. Procedure
(3) In guided practice the teacher with explanation
gives the correct answers for the questions.
(4) In independent practice section open ended
questions are asked.
The teacher explains the term Carotenoids and the
different types of Carotenoids. The teacher introduces
Beta carotenes and lutein. The teacher gives the general
formulae for carotene and Xanthophyll. Teacher
introduces the new concept Phycoblins. And explain the
functions the functions of Carotenoids
Modelling
Checking for
understanding For checking the understanding of the students the
teacher asks multiple choice questions.
After asking the questions the teacher would explain the
content once again for the students for thorough
understanding. Than the teacher explains the answers for
all questions which was asked.
Guided practice
In Independent practice the teacher ask small questions
like : (1) What are carotenoids?
Independent (1) Which pigment is responsible for the yellow colour
of the autumn leaves? practice and
closure of the lesson (2) What are the functions of Carotenoids?
(3) What are Phycobilins?
(4) Why Carotenoids appears in orange and yellow
colour other than green colour ?
(5) Which pigment is responsible for the colour of
Pumpkin, Tomato etc .
LESSON TRANSCRIPT 7
PHOTOSYNTHETIC UNIT
Author : School : Subject : Standard :
Content Objective
Students should know about the photosynthetic unit. For
that the teacher with the help of a diagram explains the
concept of photosynthetic unit. The pupil should
understand analyse and organize the concept.
(1) The pupil acquires the knowledge of
photosynthetic unit. (2) The pupil understands the importance of
photosynthetic unit. Stated objective
Purpose or Goal of lesson (3) The pupil analyses the functions of photosynthetic
unit. (4) The pupil visualizes the importance of Chlorophylla
in the photochemical reaction.
(5) The pupil organizes and evaluates the importance
of Chlorophylla molecules in the process of
photosynthesis.
Anticipatory set
or Introduction
Teacher presents the lesson with the help of a diagram.
The student carefully observes the diagram and analysis
the important parts in the diagram and should arrive at a
conclusion.
The lesson photosynthetic unit is given is small steps.
(1) For checking understanding small questions are asked. Input or
Procedure (2) In guided practice the teacher explains the content once again to the students and clarifies the doubts. (3) In Independent practice small questions are asked to
the students.
(4) Here the teacher explains the correct answer to the
students.
The teacher explains the concept of photosynthetic unit in
simple language so that everyone can grasp the concept
easily. Teacher introduces the new terms like P700, P680,
P870, Reaction centre, Light harvesting pigment molecule,
Core molecule, Antennae molecules etc. The students’
tries to understand the concepts if they have doubts the
students can clarify their doubts.
Modelling
Checking for The teacher asks small questions to check the
understanding of the students. understanding
In guided practice the teacher explains the content once
again to the students and clarifies their doubts. Teacher
explains to the answers to the questions which were
asked.
Guided practice
In independent practice section divergent questions are
asked to the students so that the skill of thinking will be
increased. The questions are ;
(1) Differentiate between reaction centre and light
harvesting pigment molecule? Independent (2) Why do photochemical reactions takes place only in practice
And
the reaction centre? Closure of the (3) What are the different types of light harvesting Lesson
pigment molecule?
(4) Differentiate between core molecule and antennae
molecule?
LESSON TRANSCRIPT 8
NATURE OF LIGHT
Author : School : Subject : Standard :
The student should know about the Nature of light. The
solar spectrum is also explained to the students so that
they should know the importance of visible spectrum in
the solar spectrum.
Content Objective
Stated objective
Purpose or Goal of lesson
(1) The pupil acquires the knowledge about the nature
of light.
(2) The pupil tries to understand about solar
spectrum.
(3) The pupil analyse the importance of visible
spectrum in the process of photosynthesis.
(4) The pupil organizes and classifies the new
knowledge.
(5) The pupil evaluates the function of solar spectrum.
The teacher explains the Nature of light with the help of a
diagrammatic sketch of the solar spectrum and the visible
spectrum.
Anticipatory set or
Introduction
Input or Procedure
(1) Teacher presents the content with the help of a
diagram.
(2) For checking understanding small multiple choice
questions are asked.
(3) In guided practice the content is explained once
again for the students.
(4) In independent practice small open ended
questions are asked.
The teacher explains the nature of light ie; the solar
spectrum and the wave length for each light. Along with
the explanation diagrammatical sketch is also shown for
better understanding. The teacher introduces the new
terms such as photosynthetic active radiation, photon and
quantum.
Modelling
Checking for For checking the understanding of the students the
teacher asks multiple choice questions. understanding
After asking the questions the teacher would explain the
content once again for the students for thorough
understanding. Than the teacher explains the answers for
all questions which was asked.
Guided practice
In Independent practice the teacher ask small questions
like: Independent practice and (1) Why solar spectrum is known as electromagnetic
spectrum? closure of the lesson
(2) What is visible spectrum?
(3) What are photosynthetically active radiations?
(4) Define photon?
(5) Define quantum?
LESSON PLAN 9
ABSORPTION SPECTRUM AND ACTION SPECTRUM
Author : School : Subject : Standard :
Content Objective
Plus one students will have the knowledge about
absorption and action spectrum. With the graphical
representation of absorption and action spectrum the
students build ups the knowledge in detail.
Stated objective
Purpose or Goal of lesson
(1) The pupil acquires the knowledge of Absorption
and Action spectrum.
(2) The pupil understands the concept.
(3) The pupil analyses the importance of Absorption
and Action spectrum
(4) The pupil organizes, evaluates and thus classifies
the difference between Absorption and Action
spectrum.
(5) The pupil generalizes the importance of Absorption
and Action spectrum in the process of
Photosynthesis.
Anticipatory set or
Introduction
Teacher explains the concept of Absorption and Action
spectrum with the graphical representation. The students’
observation will be increased when they find out the
difference between Absorption and Action spectrum.
Input or
Procedure
The lesson is prepared in different phases:
1. The concept is given in simple language for clear
understanding graphical representation of
Absorption and Action spectrum is shown.
2. Teacher guides the students to learn the lesson
with divergent questions.
3. Teacher explains the questions through guided
practice.
4. Students learn the lesson through thought
provoking questions.
Modelling
Teacher first shows the graphical representation of
absorption spectrum and motivates the students to define
it. Like that Action spectrum is also taught.
Checking for understanding
The teacher after explaining the concept asks question to
check the understanding of the students. Small multiple
choice questions are asked so that if the students had
understood the concept can answer easily.
Guided Practice
After asking questions if the students didn’t answer the
questions the teacher explains the answers with suitable
examples. Here in guided practice the teacher explains
the concept once again for the students.
In Independent practice small open ended questions are
asked. If the students have analyzed the concept the
students will be able to answer the questions. The
questions are:
Independent practice
And
Closure of the 1. Explain absorption spectrum? Lesson
2. What is Action spectrum?
3. How does the rate of photosynthesis measured?
4. Why does maximum rate of photosynthesis takes
place in red light?
APPENDIX- VI
LIST OF EXPERTS
1. Sophiammal Nettar, Head of the Botany Department, Fatima Matha
National College, Kollam.
2. Thomas Bannans, Lecturer, Botany Department, Fatima Matha
National College, Kollam.
3. Marlyn V.J., Lecturer, Botany Department, Fatima Matha National
College, Kollam.
4. Birdget Fernandez, Lecturer in Natural Science, Karmela Rani
Training College, Kollam.
5. Lali S., Principal, SNGM Training college, Thuravoor.
APPENDIX- VII
LIST OF SUBJECT TEACHERS
1. Betsy James, Government Higher Secondary School, Vallathungal.
2. Sajeev P.G., Government Higher Secondary School, Panayam.
3. Manumohan, Government Model Boys Higher Secondary School,
Kollam.
4. Biju.P., Government Model Boys Higher Secondary School, Kollam.
5. Rosamma Rajan, Government Higher Secondary School, Chathanoor.
6. Sheeja Evline Marian, Government Higher Secondary School,
Vallathungal.
7. Sherly Mathew, Government Higher Secondary School,
Anchallumoodu.
8. Lathika R. Amirta Higher Secondary School, Karicode.
9. Gopakumar, Government Higher Secondary School, Chavara.
10. Biju Lal, Government Higher Secondary School, Vellamanal.
APPENDIX- VIII
Weightage to Content of draft of Achievement test in Biology for
Standard – XII
Sl.No Content Marks %
1. Photosynthesis 5 12
2. Chloroplast – The site for Photosynthesis 7 12
3. Structure of Chloroplast 8 16
4. Photosynthetic pigment Chlorophylla 5 20
5. Structure of Chlorophylla and Chlorophyllb molecule 8 12
6. Carotenoids 5 8
7. Photosynthetic Unit 3 8
8. Nature of Light 5 8
9. Absorption and Action Spectrum 3 4
APPENDIX- IX
Weightage to Objectives of draft of Achievement test in Biology for
Standard – XII
Sl.No Objectives Marks %
1. Knowledge 14 28
2. Understanding 28 56
3. Application 8 16
Total 50 100
APPENDIX- X
Weightage to Difficulty Level of draft of Achievement test in Biology for
Standard – XII
Sl.No Difficulty Level Marks %
1. Easy 14 28
2. Average 28 56
3. Difficult 8 16
Total 50 100
APPENDIX- XI
Blue Print of the draft of Achievement test in Biology for
Standard – XII
Objectives Knowledge Understanding Application Total
Types of Questions
Content Objective
Short
Answer Objective
Short
Answer Objective
Short
Answer
Photosynthesis
1 (2) 1 (2) 1(1) 5
Chloroplast – The site for
Photosynthesis 1(2) 1(2) 1 (3) 7
Structure of Chloroplast
1 (3) 1 (2) 1 (3) 8
Photosynthetic pigment
Chlorophylla1 (1) 1 (3) 1 (2) 6
Structure of Chlorophylla
and Chlorophyllb molecule 1 (3) 1 (5) 8
Carotenoids
1(1) 1 (4) 5
Photosynthetic Unit
1 (1) 1 (2) 3
Nature of Light
1(1) 1 (4) 5
Absorption and Action
Spectrum 1(1) 1 (2) 3
15 26 9 50
APPENDIX- XII
Achievement Test in BIOLOGY on “Photosynthesis”
DRAFT
Time: 50 mts. Standard: XII Max Mark: 50
Answers can be written on separate sheet. Each question carries one mark.
I. Name the following 1. Name the universal photosynthetic pigment. 2. Which energy is converted into chemical energy during photosynthesis? 3. Which is the most important physio- biological process of the world? 4. The smallest group of pigment molecule participating in a photochemical reaction. 5. Name the green plastid seen in the cytoplasm. 6. Name the membrane bound sac like structure seen in the stroma. 7. Which molecule is attached to the 3rd carbon of the 2nd pyrrole of chlorophylla molecule? 8. What is the colour of chlorophyllb molecule? 9. Which is the pigment seen in red algae? 10. Name the pigment molecule which lies around the reaction centre.
II. Fill in the Blanks: 11. Photosynthesis takes place only in …………………….. 12. ………………… is considered as the photosynthetic apparatus. 13. The light rays reflected by chlorophyll is …………………... 14. Photosynthesis is maximum in…………………….. 15. Autotropic plants prepare their own food material through the process called …………………... 16. The membrane of chloroplast is made up of ………………….. 17. The site of dark reaction is …………………... 18. A lengthy hydrocarbon tail is attached to the prophyrin ring is called ……………………... 19. The aldehyde group increases …………………… light absorption. 20. The chloroplast pigment can be extracted and separated by ………………………. 21. The rate of photosynthesis is measured by the amount of action of ……………………….. 22. The kinetic energy of sunlight is transformed into chemical energy and stored in …………………….
23. The space inside the Chloroplast is filled with a proteinaceous fluid called ……………………………. 24. ………………………. is a molecule that absorbs light of specific wavelength in the visible spectrum. 25. The centre of tetrapyrrole is occupied by a central core of …………….. molecule. 26. Photochemical reaction takes place only in …………………. 27. All radiation emitted by sun constitute the ………………………. 28. The graphic representation of absorption maxima against wavelength is ………….
III. Match the Following: 29. Light reaction (a) Phycocyanin 30. Photon (b) C55H72O5N4Mg 31. Carotene (c) quantum 32. Cholorophyllb (d) C40H56 33. Photochemical reaction (e) C40H56O2
34. Chlorohylla (f) granum 35. Cyanobacteria (g) Cholorophylla
(h) C55H70O6N4Mg
IV. Answer in One Sentence: 36. Why photosynthesis doesn’t takes place in the roots of the plants? 37. Which pigment is responsible for the colour of pumpkin and tomato? 38. It is not advisable to keep plants in the bedroom during night. Why? 39. Why light reaction takes place only in the granum of chloroplast? 40. Why the solar spectrum is called the electromagnetic spectrum? 41. When we maintain aquarium we keep green plants in it. Why? 42. Why the leaves appear green in colour? 43. Why Chloroplast are located at the periphery of the cytoplasm? 44. Does all the pigment molecule absorbs light energy in the photosynthetic unit? 45. Why maximum rate of photosynthesis doesn’t takes place in Cholorophyllb
molecule? 46. Which pigment is responsible for the yellow colour of autumn leaves? 47. When does maximum rate of photosynthesis takes place? 48. During rainy season does the plant prepares the food materials? 49. How does Chlorophylla and Cholorophyllb molecule differs in their solubility? 50. Does the coloured plants prepare their own food materials or they depends upon Other green plants?
(50 X 1 = 50)
APPENDIX- XIII
SCORING KEY FOR DRAFT OF ACHIEVEMENT TEST
1. Chloroplast.
2. Light energy.
3. Photosynthesis.
4. Photon
5. Chloroplast.
6. Thylakoid or lamellae.
7. Methyl group.
8. Olive green.
9. Phyco-erythrin.
10. Core moleculel.
11. Green plants.
12. Chloroplast
13. Green.
14. Red.
15. Photosynthesis.
16. Lipoprotein.
17. Stroma.
18. Phytol tail.
19. Blue.
20. Chromatography.
21. CO2 Utilisation.
22. Carbohydrate.
23. Stroma.
24. Pigment.
25. Mg.
26. Photocentre.
27. Solar Spectrum.
28. Absorption Spectrum.
29. Granum.
30. Quantum.
31. C40H56.
32. C55H70O6N4Mg.
33. Chlorophylla
34. C55H72O5N4Mg.
35. Phycocyanin.
36. In root there is no Chloroplast.
37. Carotenes.
38. During night due to respiration the amount of CO2 increases.
39. Quantasomes are present only in the granum.
40. Solar radiation is electromagnetic in nature.
41. To maintain CO2 & O2 equilibrium.
42. Leaves reflect green light.
43. It helps in the diffusion of CO2 required for photosynthesis.
44. Yes.
45. Chlorophyllb increases blue light absorption and decreases red light
absorption.
46. Lutein.
47. During noon time.
48. Yes.
49. Chlorophylla is more soluble in Petroleum ether and Chlorophyllb is soluble in methyl alcohol.
50. Coloured plants prepare their own food materials.
APPENDIX- XIV
ANSWER SHEET FOR DRAFT OF ACHIEVEMENT TEST
Name :
Standard : Name of the School : Name the Following 1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Fill in the Blanks
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
Match the Following
29.
30.
31.
32.
33.
34.
35.
Answer in one sentence
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
APPENDIX- XV
Achievement Test in BIOLOGY on “Photosynthesis”
Time: 25 mts. Standard: XII Max Mark: 25 Answers can be written on separate sheet. Each question carries one mark.
I. Name the following 1. Name the universal photosynthetic pigment. 2. Which is the most important physio- biological process of the world? 3. The smallest group of pigment molecule participating in a photochemical reaction.
II. Fill in the Blanks: 4. The light rays reflected by chlorophyll is …………………... 5. Photosynthesis is maximum in…………………….. 6. Autotropic plants prepare their own food material through the process called …………………... 7. The membrane of chloroplast is made up of ………………….. 8. The site of dark reaction is …………………... 9. A lengthy hydrocarbon tail is attached to the prophyrin ring is called …………. 10. The aldehyde group increases …………………… light absorption. 11. The chloroplast pigment can be extracted and separated by ………………………. 12. The rate of photosynthesis is measured by the amount of action of …………… 13. The kinetic energy of sunlight is transformed into chemical energy and stored in …………………….
III. Match the Following: 14. Light reaction (a) Phycocyanin 15. Photon (b) C55H72O5N4Mg 16. Photochemical reaction (c) quantum 17. Chlorohylla (d) granum 18. Cyanobacteria (e) Cholorophylla
(f) C55H70O6N4Mg IV. Answer in One Sentence:
19. Why photosynthesis doesn’t takes place in the roots of the plants? 20. Which pigment is responsible for the colour of pumpkin and tomato? 21. It is not advisable to keep plants in the bedroom during night. Why? 22. Why light reaction takes place only in the granum of chloroplast? 23. Why the solar spectrum is called the electromagnetic spectrum? 24. When we maintain aquarium we keep green plants in it. Why? 25. Why the leaves appear green in colour?
(25 X 1 = 25)
APPENDIX- XVI
SCORING KEY FOR ACHIEVEMENT TEST
1. Chloroplast.
2. Photosynthesis.
3. Photon
4. Green
5. Red.
6. Photosynthesis.
7. Lipoprotein.
8. Stroma.
9. Phytol tail.
10. Blue.
11. Chromatography.
12. CO2 Utilisation.
13. Carbohydrate.
14. Granum.
15. Quantum.
16. Chlorophyll.
17. C55H72O5N4Mg.
18. Phycocyanin.
19. In root there is no Chloroplast.
20. Carotenes.
21. During night due to respiration the amount of CO2 increases.
22. Quantasomes are present only in the granum.
23. Solar radiation is electromagnetic in nature.
24. To maintain equilibrium.
25. Leaves reflect green light.
APPENDIX- XVII
ANSWER SHEET
Name :
Standard : Name of the School : Name the Following 1.
2.
Fill in the Blanks
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
Match the Following
13.
14.
15.
16.
17.
18.
Answer in One Sentence
19.
20.
21.
22.
23.
24.
25.