Apoptotic phenomena during porcine placentation Área Microscopía Electrónica Facultad Agronomía y Veterinaria Universidad Nacional de Río Cuarto Río Cuarto,

Apoptotic phenomena during Apoptotic phenomena during porcine placentationporcine placentation

Apoptotic phenomena during Apoptotic phenomena during porcine placentationporcine placentation

Área Microscopía Electrónica Facultad Agronomía y VeterinariaUniversidad Nacional de Río Cuarto Río Cuarto, Córdoba. ARGENTINA

Dra Cecilia Merkis, Mic Andrea Cristofolini and Dra Mirta Koncurat

REDVET Revista electrónica de Veterinaria REDVET Rev. electrón. vet. - http://www.redvet.es Vol. VIII, Nº 1, Enero/2007http://www.veterinaria.org/revistas/redvet/n010107.html

REDVET: 2007, Vol. VIII Nº 1 Recibido: 11 Diciembre 2006 / Referencia: 010710 / Aceptado: 30 Diciembre 2006 / Publicado: 01

Enero 2007Esta presentación DIVULGATIVA está disponible en

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Programmed cell death or Apoptosis is involved in many physiological processed including tissue homeostasis, embryonic development, and the immune response.

Programmed cell death or Apoptosis is involved in many physiological processed including tissue homeostasis, embryonic development, and the immune response.

Introduction

REDVET Revista electrónica de Veterinaria 2007 Vol.VIII Nº1

Apoptotic phenomena during porcine placentationhttp://www.veterinaria.org/revistas/redvet/n010107/010705.pdf


Cell

Apoptotic wayNecrotic way

In response to signals, the cell

induces a programmed cascade of events that

results in the destruction of the

cell.

Apoptotic bodiesDestruction cell




Efector Caspase

Effector Cell

Ligans

Iniciator Caspase

Receptor

Cytoskeleton




Efector Caspase

Nucleus

Cytoplasm

DNA




The aim of this study was to determine the expression of FAS/APO1 in empty uterus and placental samples from different days of gestation.

The aim of this study was to determine the expression of FAS/APO1 in empty uterus and placental samples from different days of gestation.

Objectives




Placentas or reproductive tracts were recovered within 1h postpartum at Rio Cuarto slaughterhouse, ARGENTINE.

--

Histological sections of the twenty porcine placenta of +/- 28, 60 y 114 days, fixed in formaldehyde 10% buffer phosphate and embedded or not in paraffin, were studied.

--- Determination of gestational age (embryos/fetus longitude) - Survey of embryos/fetus - Weight of embryos/fetus

Materials and Methods




The analyses of expression of FAS/APO 1 receptor in trophoblastic cellular surfaces, by immunoperoxidase technique, were made with the following antibodies:

The analyses of expression of FAS/APO 1 receptor in trophoblastic cellular surfaces, by immunoperoxidase technique, were made with the following antibodies:

FAS B-10 that recognizes FAS and other members of the super family of TNFR-1.

FAS C-20 that recognizes FAS and doesn’t present cross reactions with other TNFR-1.




FAS B-10

Samples Villi

Empty uterus (++)

Placenta 28 days (+++)

Placenta 60 days (++)

Placenta 114 days (+)

FAS C-20

Samples Villi

Empty uterus (+)



Placenta 114 days

(+++)

The results of immunoperoxidase were expressed in qualitative form: negative (-), positive (+), high (++) and abundant (+++).

Tables 1 and 2: Label antibodies FAS B-10 and FAS C-20 intensity, observed in villi of empty uterus and placental samples of different gestational periods.

1 2

ResultsREDVET Revista electrónica de Veterinaria 2007 Vol.VIII Nº1



Fig a and b: Microphotograph of 28 gestational days. Abundant labelling produced by antibodies FAS B-10 and FAS C-20 was observed (1000 x).

a bREDVET Revista electrónica de Veterinaria 2007 Vol.VIII Nº1



Días de Gestación

Núm

ero

de c

élul

as a

popt

ótic

as

0

50

100

150

200

250

300

350

400

450

500

550

600

28 60 114

FAS B-10

FAS C-20

GESTATIONAL DAYS

N

U

M

B

E

R

A

P

O

P

C

E

L

L

S

Fig c: Number of apoptotic cells during porcine gestation.



Fig d: Microphotograph of 28 gestational days (1000 x).



Fig e: Microphotograph of 70 gestational days (200 x).



Fig f: Microphotograph of 114gestational days (200 x).



Higher cellular reforms appear approximately one week after implantation and to the end of gestation. The first changes are produced by apoptosis receptors of TNFR-1; nevertheless to the end of pregnancy the apoptosis is due exclusively to APO-1 receptors.

Higher cellular reforms appear approximately one week after implantation and to the end of gestation. The first changes are produced by apoptosis receptors of TNFR-1; nevertheless to the end of pregnancy the apoptosis is due exclusively to APO-1 receptors.

Conclusions



Documents

Apoptotic phenomena during porcine placentation Área Microscopía Electrónica Facultad Agronomía y Veterinaria Universidad Nacional de Río Cuarto Río Cuarto,