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R&D Systems Apoptosis Catalog
USA & Canada R&D Systems, Inc. 614 McKinley Place NE
Minneapolis, MN 55413, USATEL: (800) 343-7475 (612) 379-2956
FAX: (612) 656-4400E-MAIL: [email protected]
www.RnDSystems.com
UK & Europe R&D Systems Europe Ltd. 19 Barton Lane, Abingdon Science Park
Abingdon OX14 3NB, UKTEL: +44 (0)1235 529449FAX: +44 (0)1235 533420
E-MAIL: [email protected]. RnDSystems.com
China R&D Systems China Co., Ltd. 25/G Hua Min Empire Plaza
726 West Yan An RoadTEL: (21) 52380373FAX: (21) 52371001
E-MAIL: [email protected]
International Distributors Please visit www.RnDSystems.com for a full list of international distributors.
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Tools for Cell Biology Research™
8090730_Cover.indd 1 9/23/2008 2:19:46 AM
Apoptosis Detection Kits
Apoptosis Detection KitsCell Viability & Proliferation Assays ........................................................................3-4
• TACS™ MTT ..........................................................................................................3
• TACS XTT .............................................................................................................3
• Calcein AM ..........................................................................................................4
• Resazurin .............................................................................................................4
Annexin V Assays ..............................................................................................................5
• TACS Annexin V-Biotin in situ ........................................................................5
• TACS Annexin V-Fluorescein in situ ............................................................5
DePsipher™ Mitochondrial Membrane Potential Assay .....................................6
ApoStat Apoptosis Detection Kit ................................................................................6
Caspase Colorimetric and Fluorometric Activity Assays ....................................7
PARP Activity Assay ..........................................................................................................7
Single Cell Gel Electrophoresis Assay/CometAssay™ ..........................................8
• CometAssay-Silver ............................................................................................8
• CometAssay-SYBR® ..........................................................................................8
TACS DNA Laddering Detection Kits ........................................................................9
• Ethidium Bromide Assay ................................................................................9
• Isotopic Assay.....................................................................................................9
• Chemiluminescent Assay ...............................................................................9
• Colorimetric Assay ............................................................................................9
TUNEL Labeling Kits ............................................................................................... 10-13
Light & Electron Microscope-based Kits .................................................. 10-11
Fluorescence & Flow Cytometry-based Kits ..................................................11
Microplate Reader-based Kits ..............................................................................12
Tissue type-specifi c Colorimetric Kits ...............................................................12
• CardioTACS™ .....................................................................................................12
• DermaTACS™ ....................................................................................................12
• NeuroTACS™......................................................................................................13
• TumorTACS™ .....................................................................................................13
• VasoTACS™ .........................................................................................................13
Supplemental Apoptosis Reagents .........................................................................14
Proteome Profi ler™ Apoptosis Array Kit ............................................................ 15-16
Apoptosis-related Molecule FamiliesCaspases & Regulators ........................................................................................... 18-23
Inhibitors of Apoptosis & Regulators ............................................................... 24-27
Extrinsic Pathway of Caspase Activation ........................................................ 28-62
• TNF Superfamily ....................................................................................... 28-57
• Apoptosis Adaptor Proteins ................................................................. 58-62
Intrinsic Pathway of Caspase Activation ......................................................... 63-82
• Bcl-2 Family ................................................................................................ 63-69
• Mitochondrial Proteins .......................................................................... 70-72
• Oxidative Stress ........................................................................................ 73-78
• Heat Shock Proteins ................................................................................ 79-82
Regulation of Pro- and Anti-Apoptotic Signaling Pathways ...............................................................................................83-110
• Intracellular Kinases ................................................................................ 83-96
• NFκB ...........................................................................................................97-100
• p53 Family ............................................................................................. 101-104
• Other Transcription Factors ............................................................. 105-110
Granzyme-mediated Cell Death ................................................................... 111-114
• Cytolytic Granule Proteins ............................................................... 111-114
Other Apoptosis Molecules ............................................................................ 115-117
Index............................................................................................................. 118-120
IntroductionStrategies for Apoptosis Detection ............................................................................2
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2 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373
Apoptosis, also called programmed cell death, is a coordinated and step-wise series of biochemical reactions resulting in the ordered disassembly and removal of a cell from an organism. Initiation of apoptosis leads to the activation of intracellular proteases, endonucleases, and other enzymes which proteolytically dismantle the cell by cleaving key target substrates. These intracellular events lead to signifi cant cellular changes characteristic of apoptosis, including cytoplasmic condensation and cell shrinkage, chromatin condensation, membrane blebbing, nuclear fragmentation, and the formation of apoptotic bodies which are cleared by phago-cytes. Apoptosis is a widespread biological process needed for proper organ development during embryogenesis and removal of cells damaged by exposure to pathogens or events associated with oncogenic transformation. When apoptosis occurs on schedule, neighboring cells and the organism itself are not adversely affected. Errors in apoptosis, however, such as mistiming or resistance, can have dire effects on an organism. For example, if unscheduled apoptosis occurs in irreplaceable cells, functions critical to the organism will be lost. This is the case in some
neurodegenerative disorders. In contrast, cells that resist apoptosis may cause developmental abnormalities or unregulated cell growth. As is the case in some cancers, cells may contain and accumulate mutations but will continue to undergo DNA replication and divide due to defects in apoptotic signaling pathways. Therefore, apoptotic fi delity is critical to the develop-ment and well-being of an organism.
Several cellular changes occur during apoptosis that allow for its detection, including changes in the phospholipid content of the outer leafl et of the plasma membrane, disruption of the electrochemical gradient across the mitochondrial membrane, activation of intracellular caspases, cleavage of Poly(ADP-ribose) Polymerase, DNA fragmentation, and activation of apoptotic signaling cascades. R&D Systems offers an extensive range of kits and reagents to optimize the detection of apoptosis in a variety of different research models. The most appropriate strategy will ultimately depend on the cell or tissue type being analyzed and the method used for inducing apoptosis.
Introduction
PROLIFERATION & VIABILITY ASSAYS
EARLY SIGNS OF APOPTOSIS
LATE EVENTS OF APOPTOSIS
DNA Fragmentation PARP Cleavage
Cell Surface Changes Disruption of Mitochondrial Membrane Potential Caspase Activation
TACS DNA Laddering Kits(pg. 9)
Resazurin(pg. 4)
Calcein AM(pg. 4)
TACS XTT(pg. 3)
TACS MTT(pg. 3)
Colorimetric/Fluorometric Assay
(pg. 7)
ApoStat(pg. 6)
PARP Activity Assay(pg. 7)
DePsipher Kit(pg. 6)
Annexin V(pg. 5)
General Kits
TACS TdT(pg. 10)
Specific Kits
TUNEL Assays(pg. 10-13)
Flow Cytometry
FlowTACS(pg. 11)
High throughput Screening
TiterTACS(pg. 12)
Tissue or Cell-typeSpecific Applications
CardioTACS
DermaTACS
NeuroTACS
TumorTACS
VasoTACS(pgs. 12-13)
FluorescenceLight
CometAssay(pg. 8)
CometAssay-Silver(pg. 8)
CometAssay-SYBR(pg. 8)
Interested in Apoptotic Signaling Pathways?
R&D Systems Proteome Profiler™ Apoptosis Array (page 15),Antibodies, Proteins, & ELISA Kits (see product listings in the Apoptosis-Related Molecule Families section of this catalog).
Strategies for Apoptosis Detection
R&D Systems Tools for Cell Biology Research™
Apoptosis Detection Kits
3For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
Cell Viability and Proliferation AssaysCell viability and proliferation assays can be used to evaluate whether apoptosis may be occurring in an experimental system. Cell viability refers to the number of healthy cells in a sample, while proliferation is the number of dividing cells. Cells undergoing apoptosis demonstrate a decrease in both cell proliferation and viability. While counting cells directly using a hemacytometer may be the most straightforward approach, there are other more rapid, quantitative, and accurate assays and reagents available for measuring cell viability and proliferation. Since proliferating cells are metabolically more active than quiescent cells, the following assays are suitable fordetermining proliferation, as well as cell viability and cytotoxicity.
TACS™ MTT AssayCells undergoing proliferation or apoptosis demonstrate an increase or decrease, respectively, in their ability to reduce the tetrazolium salt, MTT. In metabolically active cells, MTT is converted to insoluble purple formazan dye crystals. Addition of detergent solubilizes the crystals, which can then be measured by absorbance using a spectrophotometer. This microplate-based assay allows for the safe, sensitivemeasurement of cell proliferation and viability and is ideal for high throughput platforms. The rate of tetrazolium reduction is proportional to the rate of proliferation.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
Colorimetric Assay For the measurement of cell proliferation based on the reduction of the tetrazolium salt, MTT. Cells undergoing apoptosis have a decreased ability to reduce MTT.
3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)
• Microplate reader • Unfi xed cells TA5355TA5412
2500 Tests5000 Tests
TACS™ XTT AssayXTT is a modifi ed tetrazolium salt that is cleaved to form a soluble orange formazan dye in metabolically active cells. This dye can be directly measured by absorbance without an additional solubilization step. This microplate-based assay allows for the safe, sensitivemeasurement of cell proliferation and viability and is ideal for high throughput platforms. The rate of tetrazolium reduction is proportional to the rate of proliferation.
0.2
0
0.4
0.8
1.0
0.9
0.7
0.5
0.3
0.1
Corre
cted
Abs
orba
nce (
A490
nm - A
630 n
m)
250
0.6
50 75 100 125
Seeding Density (Cells/mL x 104)
Relative Cell Number Measured with XTTMurine WEHI cells were counted and serial diluted in a microplate using 100 µL of the listed densi-ties. Cells were incubated with XTT (Catalog # 4891-025-K) for 6 hours at 37° C and absorbances were read using an ELISA plate reader.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
Colorimetric Assay For the measurement of cell proliferation based on the reduction of the tetrazolium salt, XTT. Cells undergoing apoptosis have a decreased ability to reduce XTT.
2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT)
• Microplate reader • Unfi xed cells 4891-025-K 2500 Tests
Apoptosis Detection Kits
R&D Systems Tools for Cell Biology Research™
4 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373
Calcein AMCalcein AM is a non-fl uorescent, hydrophobic compound that easily permeates intact, living cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein, a hydrophilic, strongly fl uorescent compound that is retained in the cytoplasm of cells with intact membranes. Measuring fl uorescence provides an indication of viable cell number. Calcein AM is suitable for use with both cells in suspension and adherent cells to measure cell viability. It may be utilized in conjunction with fl ow cytometry, immunocytochemistry, and in vivo cell tracing techniques.
KIT SPECIES TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
Calcein AM Cell Viability Assay Multi-species • Microplate reader • Unfi xed cells 4892-010-K 1000 Tests
Resazurin Resazurin, also known as AlamarBlue®, is a non-toxic, water soluble, redox-sensitive dye that changes from its blue/non-fl uorescent state to a pink/highly-fl uorescent state upon reduction to resorufi n in viable cells. Resazurin may be added to cells at any time during the culture period, but for proliferation assays, it is optimal to add it during log-phase growth. Incubation times may vary depending on the metabolic rates of the cells being tested. Resazurin can be utilized as a simple and quantitative method for measuring cell proliferation, viability, and cytotoxicity.
1000
0
2000
5000
6000
Reso
rufin
(RFU
)
0.001
4000
3000
0.01 0.1 1
Cell Number (105)
Measurement of Cell Proliferation using ResazurinCCR2A-transfected murine Baf-3 cells were grown in the presence of Resazurin (Catalog # AR002). Resorufi n fl uorescence was read in a fl uorescent plate reader.
KIT DESCRIPTION CATALOG # SIZE
Resazurin For use with Cell Proliferation/Viability Assays AR002 100 mL
Apoptosis Detection Kits
5For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
Annexin V AssaysEarly in the apoptotic process, the characteristic cell surface phospholipid asymmetry is disrupted. This leads to the exposure of phosphatidylserine (PS) on the outer leafl et of the cell membrane. Annexin V is an anticoagulant protein that preferentially binds PS and, when conjugated to a reporter molecule, can be used as an indicator of apoptosis. The Annexin V conjugate may be used in fl ow cytometry or in other in situ detection methods to facilitate the rapid and early detection of cell surface changes associated with apoptosis. Annexin V binding to PS occurs only in dying cells, is calcium-dependent, and reversible. Each Annexin V molecule binds approximately 50 exposed PS monomers.
Annexin V
Induction of Apoptosis
Annexin V Binding
Plasma Membrane
phosphatidylserine
phosphatidylserinePhospholipid Flipping
Normal Cell Apoptotic Cell
Ca2+Ca2+
Ca2+
Schematic Representation of Apoptosis-induced Membrane Changes Recognized by Annexin VReorganization of the plasma membrane occurs early in the apoptotic process disrupting phospholipid asymmetry and leading to the exposure of phosphatidylserine on the outer leafl et of the cytoplasmic membrane. In the presence of calcium, Annexin V binds to phosphatidylserine allowing apoptotic cells to be easily identifi ed by fl ow cytometry or fl uorescence microscopy.
ViableViable
EarlyApoptotic
Late Apoptotic
EarlyApoptotic
Late Apoptotic
Prop
idiu
m Io
dide
100
101
100
102
103
104
101 102 103
Annexin V-FITC
Prop
idiu
m Io
dide
100
101
100
102
103
104
101 102 103
Annexin V-FITC
Detection of Apoptotic Dexamethasone-treated Thymocytes by Annexin V StainingThymocytes were left untreated (A) or treated with 100 nM dexamethasone for 15.5 hours (B) and then stained using Annexin V-FITC and propidium iodide provided in the Annexin V-FITC Apoptosis Detection Kit (Catalog # TA4638). The combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Analysis courtesy of Dr. C.M. Knud-son, Howard Hughes Medical Institute, St. Louis, MO.
KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
TACS Annexin V - Biotin in situ Streptavidin-Fluorochrome Conjugate (not provided)
• Flow Cytometry• Fluorescence microscopy
• Fixed Cells* TA4619 100 Tests
TACS Annexin V - Fluorescein in situ Fluorescein • Flow Cytometry• Fluorescence microscopy
• Unfi xed cells TA4638TA5532
100 Tests250 Tests
*Cells are fi xed after labeling with Annexin V-Biotin.
BA
R&D Systems Tools for Cell Biology Research™
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DePsipher™ Mitochondrial Membrane Potential AssayDisruption of the electrochemical gradient across the mitochondrial membrane is one of the fi rst intracellular changes to occur following the onset of apoptosis. The DePsipher Assay uses a lipophilic cation to identify changes in the potential of the mitochondrial membrane.In normal, healthy cells, the DePsipher reagent aggregates in the mitochondria to form an orange fl uorescent compound. In apoptotic cells, when the mitochondrial membrane potential is disrupted, the DePsipher reagent remains in its monomeric form and fl uoresces green. These changes in fl uorescence can be monitored by either microscopy or fl ow cytometry. The DePsipher Assay Kit is useful for evaluating cellular viability, estimating the effect of drugs or other cytotoxic treatments on cells, and detecting early apoptosis in known models.
Identifi cation of Apoptotic INT407 Cells using the DePsipher KitINT407 human embryonic intestinal cells were treated with 25µM etoposide for 8 hours and then incubated with the DePsipher reagent in reaction buffer for 30 minutes (both provided in the DePsipher Kit, Catalog # TA700). Healthy cells (containing red aggregates) were differentiated from apoptotic cells (containing only green monomers, indicated by the blue arrow) by fl uorescence microscopy.
KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
DePsipher™ Assay Lipophilic cation • Flow Cytometry• Fluorescence Microscopy
• Unfi xed Cells TA700 100 Tests
ApoStat Apoptosis Detection KitApoStat is designed to identify and quantitate caspase activity in apoptotic cells by fl ow cytometry. Cells undergoing apoptosis are irreversibly labeled with a cell permeable, FITC-conjugated pan-caspase inhibitor (ApoStat). Any unbound reagent diffuses out of the cell and is washed away. Cells are then analyzed by fl ow cytometry for the presence of bound reagent. Increased fl uorescence is an indicator of caspase activity within cells. The detection of active caspases by fl ow cytometry is a rapid method for identifying cells undergoing apoptosis.
47.8% Positive Events
10.2% Positive Events
Positive Staining Region
Rela
tive C
ell N
umbe
r
101100 102 103 104
ApoStat Staining
Detection of Caspase Activation in Jurkat CellsHuman Jurkat leukemic T cells were cultured in the absence (green histogram) or presence (purple histogram) of 1 µM staurosporine for 3.5 hours and then stained with ApoStat (Catalog # FMK012) for 30 minutes. The cells were harvested, washed, and assayed by fl ow cytometry to determine the percentage of cells expressing active caspases.
KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
ApoStat Assay FITC-VD-FMK FITC • Flow Cytometry • Cultured cells FMK012 100 Tests
Apoptosis Detection Kits
7For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
Caspase Colorimetric & Fluorometric Activity AssaysThe Caspase Colorimetric and Fluorometric Activity Assays* can be used to determine the enzymatic activity of specifi c caspases in apoptotic cells. Cell lysates are tested for protease activity by the addition of a caspase-specifi c peptide substrate that is conjugated to a colorimetric or a fl uorescent reporter molecule. Cleavage of the peptide by a specifi c caspase releases the chromophore or the fl uorochrome which can subsequently be measured spectrophotometrically. The fl uorochrome can be excited to emit fl uorescence at a specifi c wavelength and detected by a fl uorimeter or a fl uorescence microplate reader. The colorimetric or fl uorometric signal detected in these assays is directly proportional to the enzymatic activity of the caspase. *Please refer to the product listing under the Caspases & Regulators section of this catalog for colorimetric and fl uorometic activity assays available for individual caspases.
PARP Activity AssayPoly (ADP-ribose) Polymerase (PARP) is an abundant enzyme present in all somatic cells that is activated upon DNA damage. It attaches to regions of damaged DNA and catalyzes the NAD-dependent ADP-ribosylation of itself and adjacent nuclear proteins. Poly ADP-ribosylation plays a role in regulating the activities of proteins involved in DNA repair, genomic stability, and cell cycle arrest. During apoptosis, PARP is specifi cally cleaved by caspases and is no longer activated by DNA damage, preventing apoptotic cells from repairing their DNA. The PARP Universal Colorimetric Assay measures the activity of PARP in cells and tissues by detecting the incorporation of biotinylated poly(ADP-ribose) onto histone proteins. This assay is useful for testing whether damaged DNA is from non-apoptotic cells or to assay the effectiveness of PARP inhibitors.
Histones
PARP Cleavage ADP-ribosylation
DNA Damage
Sites of damage
poly(ADP-ribose)
Apoptosis DNA Repair
Repaired DNADNA Fragmentation
PARP
PARP
PARP PARPPARP
PARPPARP
PARP
PARPPARP
PARPPARP
X
X
XX
X
X
XX
XX
X
X
X
XX
X
X
X
PAR P PAR P
PAR P
Schematic Representation of the Function of PARP Following DNA DamageFollowing DNA damage, PARP catalyzes the ADP-ribosylation of itself and adjacent nuclear proteins. This modifi cation is involved in signaling DNA damage and aiding in DNA repair in normal, viable cells (right). During apoptosis, PARP is cleaved by caspases and can no longer be activated by DNA damage, preventing apoptotic cells from repairing their DNA (left).
KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
PARP Universal Colorimetric Assay
TACS-Sapphire™ • Colorimetric Assay • Unfi xed cells• Fresh tissues
4677-096-K 96 Tests
R&D Systems Tools for Cell Biology Research™
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Single Cell Gel Electrophoresis Assay/CometAssay™The CometAssay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage. The principle of the assay is based upon the ability of denatured cleaved DNA fragments to migrate out of the cell under the infl uence of an electric potential (the “comet tail”), whereas undamaged supercoiled DNA remains within the confi nes of the cell membrane (the “comet head”) when a current is applied. The method involves the immobilization of cells in a bed of low melting point agarose, followed by gentle cell lysis. After treatment with alkali to denature the DNA and hydrolyze sites of damage, the samples are submitted to electrophoresis. Samples are subsequently stained with a fl uorescent DNA-intercalating dye or silver stain and visualized using the appropriate microscope.
CometAssay-Silver
Visualization of DNA Damage using the CometAssay and Silver StainingMurine WEHI 7.1 lymphoma cells were treated with potassium permanganate and processed using the CometAssay – Silver Staining Kit (Catalog # TA4251) under alkali conditions. Following electrophoresis,samples were fi xed in cold ethanol and air dried. After drying, the slides were stained as recommended in the kit instructions and analyzed by microscopy. DNA damage is indicated by a “comet tail” shape and the overall migration pattern of the DNA.
KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
CometAssay – Silver Silver • Light microscopy • Unfi xed cells• Fresh tissues
TA4251 50 Tests
CometAssay-SYBR®
Visualization of DNA Damage using the CometAssay – SYBR KitMurine WEHI 7.1 lymphoma cells were treated with potassium permanganate and DNA fragmentation was visualized using the CometAssay – SYBR Kit (Catalog # TA800) under alkali conditions. Following electro-phoresis the samples were fi xed and dried. The slides were subsequently stained following the instructions provided in the kit, and the cells were analyzed by microscopy. Undamaged DNA does not migrate far from the origin, while damaged DNA appears as a fl uorescent “comet tail”.
KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
CometAssay – SYBR® SYBR Green I • Fluorescence microscopy • Unfi xed cells• Fresh tissues
TA800 50 Tests
Apoptosis Detection Kits
9For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
TACS™ DNA Laddering Detection KitsDNA laddering is a hallmark of apoptotic cells. It is caused by internucleosomal cleavage of the DNA by an endonuclease called Caspase activated DNase (CAD) or DNA Fragmentation Factor 40 (DFF40) which is activated during the later stages of apoptosis. Cleavage of the DNA by CAD/DFF40 forms a characteristic ladder of DNA monomers (180-200 bp) and multimers. R&D Systems offers four different DNA Laddering Kits that allow DNA fragmentation to be detected by agarose gel electrophoresis. DNA is visualized by either luminescent or colorimetric-based assays, by ethidium bromide staining, or by isotope-labeling. Kit selection depends on the number of cells available and the percentage of cells that are apoptotic.
Label & Sample Requirements
Untreated IR treated
Detection of DNA Laddering by Isotopic Labeling of DNA from IR-treated ML-1 CellsDNA from human ML-1 myeloblastic leukemia cells that were either untreated or treated with ionizing radiation (20 Gy) and allowed to recover 48 hours, was isotopically labeled according to the protocol in the TACS DNA Laddering Detection Kit (Catalog # TA4631), and then separated by agarose gel electrophoresis. The gel was subsequently fi xed, dried, and exposed to fi lm for 2 hours. DNA laddering in the cells that were treated with IR is indicative of apoptosis.
KIT LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
Ethidium Bromide Assay Ethidium bromide (not provided) • Gel electrophoresis • Unfi xed cells• Fresh tissues
TA4630 20 Tests
Isotopic Assay [α-32P]-label (not provided) • Gel electrophoresis • Unfi xed cells• Fresh tissues
TA4631 20 Tests
Chemiluminescent Assay PeroxyGlow™ • Gel electrophoresis • Unfi xed cells• Fresh tissues
TA4632 20 Tests
Colorimetric Assay Blue Membrane Solution • Gel electrophoresis • Unfi xed cells• Fresh tissues
TA4633 20 Tests
Ethidium Bromide
Minimum Cell Number 1 x 106
Sensitivity 20% apoptotic
Colorimetric Assay
Minimum Cell Number 1 x 105
Sensitivity 10% apoptotic
Chemiluminescent Assay
Minimum Cell Number 1 x 105
Sensitivity 2% apoptotic
Isotopic Assay
Minimum Cell Number 1 x 105
Sensitivity 1% apoptotic
R&D Systems Tools for Cell Biology Research™
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TUNEL Labeling KitsApoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). R&D Systems offers kits designed for several different formats including light and fl uorescence microscopy, fl ow cytometry, and colorimetric microplate reader-based assays. We also offer colorimetric kits optimized for specifi c tissue types including cardiac, skin, neuronal, tumor, and vascular cells and tissues. For more detailed information, please see our website at www.RnDSystems.com/go/TUNEL.
Light & Electron Microscope-based Kits
Detection of Apoptosis in Post-weaning Mouse Breast Tissue using TACS™ TdT DAB KitPost-weaning mouse breast tissue was fi xed in 10% neutral buffered formalin, embedded in paraffi n, and sec-tioned at fi ve microns. Deparaffi nized and rehydrated sections were subsequently processed using the TACS TdT DAB Kit protocol (Catalog # TA4625) and visualized by light microscopy. Apoptotic nuclei stain brown (arrows) using this protocol.
DNA Fragmentation in Mouse Lung Parenchyma Tissue Detected using the TACS•XL® Blue KitMouse lung parenchyma tissue was fi xed and embedded in paraffi n. Five micron sections were prepared and immobilized on slides. The tissue was labeled as described in the TACS•XL protocol (Catalog # TA300) and ana-lyzed by light microscopy for DNA fragmentation indicated by the blue stained cells (arrows). Data was provided courtesy of F. D’Agostini, University of Genova Via A. Pastore, Genova, Italy.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
TACS TdTIn situ Apoptosis Detection Kit - Core Reagents
Provides the core reagents necessary for the TUNEL reaction. Includes 10X TdT Labeling Buffer, TdT-dNTP mix, TdT Enzyme, 10X TdT Stop Buffer, Streptavidin-HRP, 50X Co2+, 50X Mg2+, and 50X Mn2+.
Biotinylated Nucleotides and Streptavidin-HRP. User supplied label
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA4628 30 Tests
TACS TdTIn situ Apoptosis Detection Kit - DAB
A complete kit supplied with Streptavidin-HRP, DAB, Methyl Green counterstain, and reagents necessary for generating positive controls.
Biotinylated Nucleotides & Streptavidin-HRP/DAB
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA4625 30 Tests
TACS TdTIn situ Apoptosis Detection Kit - Blue Label
A complete kit supplied with Streptavidin-HRP, TACS BlueLabel™, Nuclear Fast Red counterstain, and reagents necessary for generating positive controls.
Biotinylated Nucleotides & Streptavidin-HRP/TACS BlueLabel.
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA4626 30 Tests
TACS TdTIn situ Apoptosis Detection Kit - Replenisher
Provides limiting core reagents necessary for the TUNEL reaction. Includes Proteinase K Solution, TdT-dNTP Mix, TdT Enzyme, Streptavidin-HRP, 50X Co2+, 50X Mg2+, and 50X Mn2+.
Biotinylated Nucleotides & Streptavidin-HRP
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA4629 30 Tests
Apoptosis Detection Kits
11For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
Light & Electron Microscope-based Kits continued
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
TACS•XLIn situ Apoptosis Detection Kit - Basic
Provides the core reagents necessary for the TUNEL reaction. Includes Cytonin, Proteinase K Solution, 10X TdT Labeling Buffer, 10X TdT Stop Buffer, B-dNTP Mix, TdT Enzyme, Streptavidin-HRP, Streptavidin Diluent, and Anti-BrdU antibody.
Brominated Nucleotides, Biotinylated anti-BrdU, Streptavidin-HRP. User supplied label
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA100 30 Tests
TACS•XL In situ Apoptosis Detection Kit - DAB
A complete kit supplied with biotin-conjugated anti-BrdU, Streptavidin-HRP, DAB, Methyl Green counterstain, and reagents necessary for generating positive controls.
Brominated Nucleotides, Biotinylated anti-BrdU antibody, Streptavidin-HRP/DAB
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA200 30 Tests
TACS•XL In situ Apoptosis Detection Kit - Blue Label
A complete kit supplied with biotin conjugated anti-BrdU, Streptavidin-HRP, TACS Blue Label, Nuclear Fast Red counterstain, and reagents necessary for generating positive controls.
Brominated Nucleotides, Biotinylated anti-BrdU antibody, Streptavidin-HRP/TACS BlueLabel.
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA300 30 Tests
TACS•XL In situ Apoptosis Detection Kit - Replinisher
Provides the limiting, core reagents necessary for the TUNEL reaction. Includes Proteinase K Solution, B-dNTP Mix, TdT Enzyme, Streptavidin-HRP, Streptavidin Diluent, and Anti-BrdU antibody.
Brominated Nucleotides, Biotinylated anti-BrdU antibody, Streptavidin-HRP.
• Light microscopy• Double labeling• Electron
microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA400 30 Tests
Fluorescence & Flow Cytometry-based Kits
0
100
300
R2
R1
500
200
100 200150 250
0
100
300
R2
R1
500
200
100 200150 250
Analysis of DNA Fragmentation in Cyclohexamide- or Dexamethasone-treated Murine ThymocytesMurine thymocytes were treated with 10 µg/mL cyclohexamide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled accordingto the FlowTACS protocol (Catalog # TA5354), and analyzed by fl ow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
TACS TdT In situ Apoptosis Detection Kit - Fluorescein
A complete kit supplied with Streptavidin-Fluorescein and all the reagents necessary for generating positive controls.
Biotinylated Nucleotides and Streptavidin-Fluorescein
• Fluorescence microscopy
• Double labeling• Electron microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells and
tissues• Resin-embedded cells and
tissues
TA4627 30 Tests
FlowTACS™ In situ Apoptosis Detection Kit
A complete kit that comes with all the necessary reagents required for labeling samples and generating positive controls for analysis in Flow Cytometry.
Biotinylated Nucleotides and Streptavidin-Fluorescein
• Flow Cytometry• Fluorescence
microscopy• Double labeling
• Fixed cells TA5354 60 Tests
A B
R&D Systems Tools for Cell Biology Research™
12 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373
Microplate Reader-based Kits
0
Unt
reat
ed C
ells
20,0
00 C
ells
40,0
00 C
ells
60,0
00 C
ells
80,0
00 C
ells
Unl
abel
ed C
ells
Nuc
leas
e
trea
ted
Cells
1.6
1.4
1.2
1.0
0.8
0.6
Abso
rban
ce (4
50 n
m)
0.4
0.2
Detection of Apoptosis in Staurosporine-treated ML-1 CellsHuman ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fi xed and labeled according to the protocol in the TiterTACS Kit (Catalog # TA600) prior to colorimetric analysis. The reac-tion was stopped with 2N HCl.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
TiterTACS™ In situ Apoptosis Detection Kit
A complete kit supplied with TACS-Sapphire optimized for colorimetric detection in 96-well plates, and all the reagents necessary for generating positive controls.
Biotinylated Nucleotides and Streptavidin-HRP/TACS Sapphire
• Microplate reader • Suspension or monolayer cell cultures
TA600 96 Tests
Tissue Type-Specifi c Colorimetric Kits
CardioTACS™
Visualization of an Apoptotic Rat Cardiac Myocyte using the CardioTACS In situ Detection KitRat heart tissue was fi xed in 4% formaldehyde overnight and paraffi n-embedded. Five micron sections were prepared and placed onto glass microscope slides. Samples were processed following the CardioTACS Kit protocol (Catalog # TA5353) and visualized by light microscopy. An apoptotic cardiac myocyte is indicated by the arrow. Photo was provided courtesy of Dr. J. Zhang, FDA.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
CardioTACS™ In situ Apoptosis Detection Kit
A complete kit supplied with TACS Blue Label and Nuclear Fast Red counterstain. Optimized for labeling samples and generating positive controls in cardiac tissues.
Biotinylated Nucleotides and Streptavidin-HRP/TACS BlueLabel
• Light microscopy• Double labeling• Electron microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells
and tissues• Resin-embedded cells and
tissues
TA5353 30 Tests
DermaTACS™
A
B
C
Detection of DNA Fragmentation in the UVB-irradiated Human Skin Model, EpiDerm™ using the DermaTACS In situDetection KitSkin tissue samples, untreated (A), treated with 180 mJ/cm2 irradiation (B), or with TACS-Nuclease (C), were fi xed in 10% buffered formalin one hour following treatment. Fixed samples were embedded in paraffi n and six micron sections were labeled for DNA fragmentation using the DermaTACS Kit protocol (Catalog # TA4829). The dark blue stained cells exhibit the punctuate morphology indicative of apoptosis (B; arrow). The TACS-Nuclease treated sample shows the diffuse blue staining of fragmented, uncondensed DNA (C; arrows). Samples were provided courtesy of Dr. Patrick Hayden, MatTek Corporation.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
DermaTACS™ In situ Apoptosis Detection Kit
A complete kit supplied with TACS Blue Label and Red Counterstain C. Optimized for labeling samples and generating positive controls in skin tissues.
Brominated Nucleotides and Biotinylated anti-BrdU, Streptavidin-HRP/TACS BlueLabel
• Light microscopy• Double labeling• Electron microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells
and tissues• Resin-embedded cells and
tissues
TA4829 30 Tests
Apoptosis Detection Kits
13For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
NeuroTACS™
Neonatal Mouse Cortex Labeled for Apoptosis using the NeuroTACS In situ Apoptosis Detection KitThe cortex of a neonatal mouse, damaged by a two hour hypoxic-ischemic insult to the forebrain, was labeled 24 hours later for in situ apoptosis (brown stained nuclei; arrows) using the NeuroTACS II Kit protocol (Catalog # TA900) and for GFAP using TACS Red label.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
NeuroTACS™ II In situ Apoptosis Detection Kit
A complete kit supplied with DAB and Blue counterstain. Optimized for labeling samples and generating positive controls in neuronal samples.
Biotinylated Nucleotides and Streptavidin-HRP/DAB
• Light microscopy• Double labeling• Electron microscopy
• Paraffi n-embedded cells and tissues
• Resin-embedded cells and tissues
TA900 30 Tests
TumorTACS™
Apoptotic Cells within a Mouse Mammary Tumor Identifi ed using the TumorTACS KitMammary tumor was fi xed in 4% paraformaldehyde overnight and paraffi n-embedded. Five micron sections were pre-pared and placed onto glass microscope slides. The sample was processed following the TumorTACS Kit protocol (Cata-log # TA5411). Brown stained nuclei indicate apoptotic cells (arrows).
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
TumorTACS™ In situ Apoptosis Detection Kit
A complete kit supplied with DAB and Methyl Green counterstain. Optimized for labeling samples and generating positive controls in tumor samples.
Biotinylated Nucleotides and Streptavidin-HRP/DAB
• Light microscopy• Double labeling• Electron microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells
and tissues• Resin-embedded cells and
tissues
TA5411 30 Tests
VasoTACS™
Visualization of Apoptotic Cells in Vascular TissueTissue from the small artery of a rat was formalin-fi xed and paraffi n-embedded following the initiation of drug-induced apoptosis. The tissue was processed according to the protocol in the VasoTACS Kit (Catalog # 4826-30-K) and visualized by light microscopy. Brown stained nuclei following counterstaining with Red Counterstain C indicate apoptotic cells (arrows). Data was provided courtesy of Dr. Jun Zhang, FDA.
KIT DESCRIPTION LABEL TESTING FORMAT SAMPLE TYPE CATALOG # SIZE
VasoTACS™ In situ Apoptosis Detection Kit
A complete kit supplied with TACS Blue Label and Red Counterstain C. Optimized for labeling samples and generating positive controls in vascular samples.
Biotinylated Nucleotides and Streptavidin-HRP/TACS BlueLabel
• Light microscopy• Double labeling• Electron microscopy
• Fixed cells• Frozen tissues• Paraffi n-embedded cells
and tissues• Resin-embedded cells and
tissues
4826-30-K 30 Tests
R&D Systems Tools for Cell Biology Research™
14 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373
Supplemental Apoptosis ReagentsREAGENT DESCRIPTION CATALOG # SIZE
4-Amino-1,8-Naphthalimide Inhibitor of poly(ADP-ribose) polymerase (PARP). Can be used with the PARP Assay. 4667-50-09 100 µL
6(5H)-Phenanthridinone Inhibits poly(ADP-ribose) polymerase (PARP) and displays immunosuppressive activity. Can be used with the PARP Assay.
4667-50-10 100 µL
6-Biotin-17-NAD Non-isotopic alternative to radiolabeled NAD for studies requiring this substrate. Can beused with the PARP Assay.
4670-500-01 500 µL
Benzamide Inhibits poly(ADP-ribose) polymerase (PARP). It is able to prevent nuclear fragmentation and apoptotic-body formation without affecting DNA fragmentation during apoptosis. Can be used with the PARP Assay.
4667-50-11 100 µL
Cell Culture Control Slides Slides with fi xed apoptotic cells to confi rm a positive reaction with any of the TACS or TACS•XL in situ apoptosis detection kits.
4800-30-21 5 Each
Cobalt Cation For optimizing TdT labeling in TACS in situ apoptosis detection kits. Cobalt is the standard cation for labeling all cell types and tissues.
4810-90-09 3 x 30 µL
CometSlide For use with the CometAssay. 4250-200-03 100 Slides
Cytonin Protease-free, saponin-based permeabilization and blocking reagent designed for use with any of the TACS or TACS•XL apoptosis detection kits.
4876-05-02 2 x 5 mL
Magnesium Cation For optimizing TdT labeling in TACS in situ apoptosis detection kits. Magnesium is useful for tissue that demonstrates high background.
4810-90-10 3 x 30 µL
Manganese Cation For optimizing TdT labeling in TACS in situ apoptosis detection kits. Manganese may increase the signal to noise ratio providing improved labeling.
4810-90-14 3 x 30 µL
NeuroPore Non-proteolytic permeabilization and blocking reagent developed for use with the NeuroTACS kit.
4820-60-01 2 x 5 mL
PARP Activated DNA May be used with the PARP Assay as an activator of Poly(ADP-ribose) Polymerase. 4667-50-06 500 µL
Peroxynitrite For use in the preparation of positive control proteins and cell lysates for immunoreactivity assays.
AR006 5 mL
Poly(ADP-ribose) Polymer/pADPr
Western blotting standard. 4336-100-01 100 µL
Proteinase K Permeabilization reagent. 4800-30-01 30 µL
Streptavidin-Fluorescein For detection of biotin incorporation with fl uorescent detection. 4800-30-14 30 µL
Streptavidin-HRP For detection of biotin incorporation with colorimetric detection. 4800-30-06 30 µL
TACS Blue Label Substrate For use with TACS in situ apoptosis detection kits. 4800-30-BL 30 Tests
TACS Red Label Substrate For use with TACS in situ apoptosis detection kits. 4825-30-RL 30 Tests
TdT Enzyme/Buffer For use with TACS in situ apoptosis detection kits. 9510-01K-EB 1000 U
TdT dNTP Mix Biotinylated nucleotides for use in TACS apoptosis detection. 4810-30-04 30 µL
Tissue Control Slides Slides with fi xed apoptotic tissues to confi rm a positive reaction with any of the TACS or TACS•XL in situ apoptosis detection kits.
4800-30-404800-30-41
2 Each5 Each
Apoptosis Detection Kits
15For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
Proteome Profi ler™ ArraysProteome Profi ler Arrays allow for the measurement of multiple proteins in a single sample. These arrays require no specialized equipment and eliminate the need for multiple immunoprecipitation/Western blot experiments. For more detailed information, please see our website at www.RnDSystems.com/go/ProteomeProfi ler.
Human Apoptosis Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE
Human Apoptosis Array Kit Contains 4 arrays - each spotted in duplicate with 35 different apoptosis-related antibodies ARY009 1 Kit
Bad, Bax, Bcl-2, Bcl-x, Pro-Caspase-3, Cleaved Caspase-3, Catalase, Claspin, Clusterin, Cytochrome c, FADD, Fas/TNFSF6, HIF-1α, HO-1/HMOX1/HSP32, HO-2/HMOX2, HSP27, HSP60, HSP70, HTRA2/Omi, cIAP-1, cIAP-2, Livin, p21/CIP1/CDNK1A, p27/Kip1, PON2, Phospho-p53 (S15), Phospho-p53 (S46), Phospho-p53 (S392), Phospho-Rad17 (S635), SMAC/Diablo, Survivin, TNF RI/TNFRSF1A, TRAIL R1/DR4, TRAIL R2/DR5, XIAP
1PC
1
2 23
3
44
5
5
6
6
0
10000
20000
30000
40000
50000
60000
Bcl-
x
Cyto
c
HSP
27
HTR
A2/
Om
i
SMAC
/D
iabl
o
XIAP
Pixe
l Int
ensi
ty
Human Phospho-RTK Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE
Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit
Contains 4 arrays - each spotted in duplicate with 42 different RTK antibodies ARY001 1 Kit
Axl, Dtk, EGF R, EphA1, EphA2, EphA3, EphA4, EphA6, EphA7, EphB1, EphB2, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FGF R1, FGF R2α, FGF R3, FGF R4, Flt-3, HGF R, IGF-I R, Insulin R, M-CSF R, Mer, MSP R, MuSK, PDGF Rα, PDGF Rβ, c-Ret, ROR1, ROR2, SCF R, Tie-1, Tie-2, TrkA, TrkB, TrkC, VEGF R1, VEGF R2, VEGF R3
A B
C
Detection of Multiple Apoptosis-related Proteins in Cell Lysates using the Proteome Profi ler Human Apoptosis Antibody ArrayA. Cell lysates from human K562 myelogenous leukemia cells were incubated with the Proteome Profi ler Human Apoptosis Antibody Array (Catalog # ARY009). The relative levels of 35 different apoptosis-related proteins were visualized simultaneously by chemiluminescent detection. PC = Positive Control B. Histogram profi les for select analytes were generated by quantifying the mean spot pixel densities from the Apoptosis Antibody Array using image analysis software. C. Analytes and their coordinate locations on the Proteome Profi ler Apoptosis Antibody Array.
COORDINATE TARGET/CONTROL
A1, A2 Positive Control
A23, A24 Positive Control
B1, B2 Bad
B3, B4 Bax
B5, B6 Bcl-2
B7, B8 Bcl-x
B9, B10 Pro-Caspase-3
B11, B12 Cleaved Caspase-3
B13, B14 Catalase
B15, B16 cIAP-1
COORDINATE TARGET/CONTROL
B17, B18 cIAP-2
B19, B20 Claspin
B21, B22 Clusterin
B23, B24 Cytochrome c
C1, C2 TRAIL R1/DR4
C3, C4 TRAIL R2/DR5
C5, C6 FADD
C7, C8 Fas/TNFSF6
C9, C10 HIF-1α
C11, C12 HO-1/HMOX1/HSP32
COORDINATE TARGET/CONTROL
C13, C14 HO-2/HMOX2
C15, C16 HSP27
C17, C18 HSP60
C19, C20 HSP70
C21, C22 HTRA2/Omi
C23, C24 Livin
D1, D2 PON2
D3, D4 p21/CIP1/CDNK1A
D5, D6 p27/Kip1
D7, D8 Phospho-p53 (S15)
COORDINATE TARGET/CONTROL
D9, D10 Phospho-p53 (S46)
D11, D12 Phospho-p53 (S392)
D13, D14 Phospho-Rad17 (S635)
D15, D16 SMAC/Diablo
D17, D18 Survivin
D19, D20 TNF RI/TNFRSF1A
D21, D22 XIAP
D23, D24 PBS (Negative Control)
E1, E2 Positive Control
R&D Systems Tools for Cell Biology Research™
16 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373
Human Phospho-MAPK Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE
Human Phospho-Mitogen Activated Protein Kinase (MAPK) Array Kit
Contains 4 arrays - each spotted in duplicate with 21 different antibodies recognizing MAPKs and other kinases.
ARY002 1 Kit
Akt1, Akt2, Akt3, Akt pan, p38δ, ERK1, ERK2, GSK-3α/β, GSK-3β, HSP27, JNK1, JNK2, JNK3, JNK pan, MSK2, p38α, p38β, p38γ, p70 S6 Kinase, RSK1, RSK2
Human Phospho-Kinase Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE
Human Phospho-Kinase Array Kit Contains 4 sets of 2 membranes - each spotted in duplicate with 46 different kinase antibodies ARY003 1 Kit
Akt (S473), Akt (T308), AMPKα1, AMPKα2, β-Catenin, Chk-2, CREB, ERK1/2, FAK, Fgr, Fyn, GSK-3α/β, Hck, HSP27, JNK pan, c-JUN, Lck, Lyn, MEK1/2, MSK1/2, eNOS, p27 (T198), p27 (T157), p38α, p53 (S15), p53 (S46), p53 (S392), p70 S6 Kinase (T229), p70 S6 Kinase (T389), p70 S6 Kinase (T421/S424), Paxillin, PLCγ-1, Pyk2, RSK1/2 (S221), RSK1/2/3 (S380), Src, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5a/b, STAT5b, STAT6, TOR, Yes
Human Phospho-Immunoreceptor Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE
Human Phospho-Immunoreceptor Array Kit
Contains 4 arrays - each spotted in duplicate with 59 different antibodies recognizing proteins involved in immunoreceptor signaling.
ARY004 1 Kit
2B4, BLAME, BTLA, CD3ε, CD5, CD6, CD28, CD84, CD229, CEACAM-1, CLEC-1, CLEC-2, CRACC, CTLA-4, DCIR, Dectin-1, DNAM-1, Fcε RII, Fcγ RIIA, Fcγ RIIIA, FcRH1, FcRH2, FcRH4, FcRH5, ILT2, ILT3, ILT4, ILT5, ILT6, Integrin β3, KIR2DL4, LAIR-1, LAIR-2, LMIR1, LMIR2, LMIR3, LMIR6, MDL-1, NKp30, NKp44, NKp46, NKp80, NTB-A, PD-1, PECAM, SHIP-1, SHP-1, SHP-2, Siglec-2, Siglec-3, Siglec-5, Siglec-7, Siglec-9, Siglec-10, SIRP-β1, SLAM, TREM-1, TREM-2, TREML1
Human Cytokine Antibody Array, Panel APRODUCT DESCRIPTION CATALOG # SIZE
Human Cytokine Array Kit, Panel A Contains 4 arrays - each spotted in duplicate with 36 different cytokine antibodies ARY005 1 Kit
C5a, CD40 Ligand, G-CSF, GM-CSF, GROα, I-309, sICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 p70, IL-13, IL-16, IL-17, IL-17E, IL-23, IL-27, IL-32α, IP-10, I-TAC, MCP-1, MIF, MIP-1α, MIP-1β, RANTES, SDF-1, Serpin E1, TNF-α, sTREM-1
Mouse Cytokine Antibody Array, Panel APRODUCT DESCRIPTION CATALOG # SIZE
Mouse Cytokine Array Kit, Panel A Contains 4 arrays - each spotted in duplicate with 40 different cytokine antibodies ARY006 1 Kit
BLC, C5a, G-CSF, GM-CSF, I-309, Eotaxin, sICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-13, IL-16, IL-17, IL-23, IL-27, IP-10, I-TAC, KC, M-CSF, JE, MCP-5, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, SDF-1, TARC, TIMP-1, TNF-α, TREM-1
Rat Cytokine Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE
Rat Cytokine Array Kit, Panel A Contains 4 arrays - each spotted in duplicate with 29 different cytokine antibodies ARY008 1 Kit
CINC-1, CINC-2α/β, CINC-3, CNTF, Fractalkine, GM-CSF, sICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-10, IL-13, IL-17, IP-10, LIX, MIG, MIP-1α, MIP-3α, RANTES, L-Selectin, Thymus Chemokine, TIMP-1, TNF-α, VEGF
Human Angiogenesis Antibody ArrayPRODUCT DESCRIPTION CATALOG # SIZE
Human Angiogenesis Array Contains 4 arrays - each spotted in duplicate with 55 different angiogenesis antibodies ARY007 1 Kit
Activin A, ADAMTS-1, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin/Plasminogen, Amphiregulin, Artemin, Coagulation Factor III/TF, CXCL16, DPPIV/CD26, EGF, EG-VEGF, Endoglin, Endostatin/Collagen 18, ET-1, FGF acidic, FGF basic, FGF-4, FGF-7/KGF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IL-1β, CXCL8/IL-8, LAP (TGF-β1), Leptin, MCP-1/CCL2, MIP-1α/CCL3, MMP-8, MMP-9, NRG1-β1/HRG1-β1, Pentraxin 3, PD-ECGF, PDGF-AA, PDGF-AB/BB, Persephin, PF4/CXCL4, PlGF, Prolactin, Serpin B5, Serpin E1/PAI-1, Seprin F1/PEDF, TIMP-1, TIMP-4, TSP-1, TSP-2, uPA, Vasohibin, VEGF, VEGF-C
17For research use only. Not for use in diagnostic procedures. www.RnDSystems.com
p53 p53
DNA Damage
xx x
x
p53P
PP
P
PP
P
Caspase Recruitment Domain (CARD)
Cytochrome c
BID
SMAC/Diablo HTRA2/Omi
Endo G
AIF
Cytochrome c
Cytochrome c
BID
Caspase-2
Cytochrome c tBID
tBIDtBID
tBID
tBID
tBID
tBID
tBID
tBID
tBID
Bcl-xL
Bcl-2
Bcl-2
Bcl-2
Bax
Bax
Bax Bad
Bad
Bad
Bad
Bad
Bcl-xL
Bcl-xL
Bax
BAK
BAK
BAK
BAK
Bax
Bax
Caspase-8,-10
Caspase-9
Caspase-3
TRAF-2
TRAF-2
TRADD
TRADDTRADD
FADD
FADDFADD
Pro-caspase-8, -10
Pro-caspase-8, -10
TRADD
RIP1
RIP1
DEDDD
Death Domain (DD) Death Effector Domain (DED)
Caspase-7
Caspase-6
IAPs
IAPs
HSPs
Target Molecules (e.g. Actin, Nuclear lamins,ICAD, PARP)
Pro-caspase-7
Pro-caspase-6Pro-caspase-9
Apoptosome
PIDDosome
APAF-1
DNA Fragmentation
ARC
PIDD
CRADD/RAIDD
Pro-caspase-2
Pro-apoptotic: Bad, Bax, BID, Noxa, PUMA, PIDD, othersAnti-apoptotic: 14-3-3, p21, others
Pro-caspase-3
14-3-3
Bad
14-3-3
FADDFADD
TNF-α
TNF RI
Fas Ligand
TRAIL
TWEAK
DR3 (or another TWEAK R)
TRAIL R1TRAIL R2
Fas
FLIP
Pro-caspase-8, -10
DD
DDCARD
Apoptosis-related Molecule Families
Apoptosis-related Molecule Families
Extrinsic & Intrinsic Pathways of Apoptotic Caspase ActivationApoptosis is initiated by an extrinsic or intrinsic death signal which ultimately leads to the activation of intracellular caspases. Multiple protein families are
involved in the signaling pathways that promote or inhibit caspase activation. The extrinsic pathway of caspase activation (green arrows) involves ligand-
binding to cell surface death receptors, belonging to the TNF receptor superfamily, while the intrinsic pathway (purple arrows) involves a loss of the
integrity of the outer mitochondrial membrane which is regulated by members of the Bcl-2 family of proteins. In addition to these two families of proteins,
numerous other intracellular proteins, kinases, and transcription factors play a role in promoting cell death, or acting to prevent it. R&D Systems has
developed and validated a wide variety of research reagents useful for the characterization of these pro- and anti-apoptotic signaling pathways.
Note: this diagram conveys a general overview of selected processes and should neither be considered comprehensive nor defi nitive. The details of these processes are understood to be subject to interpretation.
Extrinsic Pathway of Caspase Activation
Intrinsic Pathway of
Caspase Activation
Caspases & Regulators
R&D Systems Tools for Cell Biology Research™
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037318
Caspases are a family of cysteine proteases found in the cytosol
which act as the primary mediators of apoptosis. Mammalian
caspases can be subdivided into three functional groups, apoptotic
initiator caspases (Caspase-2, -8, -9, -10), apoptotic effector
caspases (Caspase-3, -6, -7), and caspases involved in inflammatory
cytokine processing (Caspase-1, -4, -5, and -12L/12S). All caspases
are synthesized as inactive zymogens containing a variable length
pro-domain, followed by a large (20 kDa) and a small subunit
(10 kDa; Figure A). The apoptotic caspases are activated upon the
receipt of either an extrinsic or an intrinsic death signal. The extrin-
sic signaling pathway is initiated by ligand binding to cell surface
death receptors followed by receptor oligomerization. The intrinsic
signaling pathway is activated by conditions such as DNA damage,
which lead to changes in the mitochondrial membrane that result
in the release of pro-apoptotic proteins, such as Cytochrome c and
Smac/Diablo. Signaling initiated by either the extrinsic or the intrin-
sic apoptotic pathway results in the processing and activation of
specific initiator caspases. These caspases subsequently cleave and
activate downstream effector caspases.
Apoptotic initiator caspases are activated in three distinct protein
complexes, the death-inducing signaling complex (DISC), the
apoptosome, and the PIDD osome. Pro - caspase -8 and
Pro-caspase-10 are recruited by way of their death effector do-
mains (DED) to ligand-bound, oligomerized death receptors
(TNF RI, Fas, DR3, TRAIL R1/DR4, TRAIL R2/DR5) by the adaptor pro-
tein, FADD. Clustering of pro-caspases in the cytosol near the death
receptor leads to the formation of the DISC and the subsequent
cleavage of Pro-caspase-8 and Pro-caspase-10. A second death sig-
naling complex is formed following the release of Cytochrome c
from the mitochondria. Cytochrome c interacts with APAF-1 in the
cytosol which recruits Pro-caspase-9 by way of its caspase recruit-
ment domain (CARD). Together these three proteins form a
complex called the apoptosome which leads to the cleavage and
activation of Caspase-9. Formation of a third protein complex leads
to the activation Pro-caspase-2. Following DNA damage, p53 in-
duces the expression of PIDD (p53-induced protein with a death
domain) which associates with the adaptor protein, CRADD/RAIDD
and Pro-caspase-2 to form the PIDDosome. Within this complex,
Pro-caspase-2 is processed and activated. Formation of the DISC,
apoptosome, or the PIDDosome leads to the autocatalytic cleavage
of the respective initiator pro-caspases at aspartic acid residues lo-
cated after the pro-domain, and in between the large and the small
subunits. Upon cleavage, mature caspases form a proteolytically
active heterotetramer consisting of two small and two large sub-
units (Figure B). These activated initiator caspases can then cleave
and activate the effector caspases which proteolytically dismantle
the cell by cleaving specific target substrates, including structural
proteins, DNA repair proteins, and proteins involved in signal
transduction pathways. Caspase activity can be negatively regu-
lated by the FLIP protein and members of the Inhibitors of
Apoptosis (IAP) family.
For more information on Caspase-related products, please visit our
website at www.RnDSystems.com/go/Caspases
Domain Organization and Activation of the Apoptotic Caspases. (A) Initiator pro-caspases consist of a large pro-domain containing either the death effector domains
(DED) or the caspase recruitment domain (CARD), followed by a large (p20) and a small (p10) subunit. Effector pro-caspases contain a significantly shorter pro-domain followed
by the p20 and p10 subunits. (B) Proteolytic cleavage of the pro-caspases at aspartic acid residues located after the pro-domain and in between the large and the small subunit
leads to the formation of a proteolytically active caspase heterotetramer.
For antibody application key, see page 120.
APAF-1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
APAF-1aa 10-214
Human None 94408 Mouse IgG2B IP, WB MAB868 100 µg
ARCRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
ARCWestern Blot Standard
Human Solution without a carrierprotein
None E. coli WBC009 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
ARCaa 176-195
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF636 100 µg
ASCANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
ASCaa 112-195
Human None Polyclonal Goat IgG WB AF3805 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 19
Detection of Pro-caspase-7 Cleavage in Human
Jurkat and Mouse DA3 Cells. Human Jurkat T cells and
mouse myeloid DA3 cells were either untreated or
treated with staurosporine (STS; 1 µM) for the indicated
times. The pro-caspase and cleaved large subunit forms
of Caspase-7 were detected by Western blot analysis us-
ing anti-human/mouse Caspase-7 monoclonal antibody
(Catalog # MAB823).
Activation of Pro-caspase-3 by Caspase-8. Recombi-
nant human Caspase-8 (Catalog # 705-C8) was
incubated with increasing amounts of Pro-caspase-3
(Catalog #731-C3) for one hour prior to the addition of
the Caspase-3 peptide substrate, DEVD-afc. Caspase-3
activity was determined by monitoring DEVD-afc cleav-
age in a fluorescence plate reader (blue line).
Background activity was measured by monitoring
DEVD-afc cleavage by Pro-caspase-3 in the absence of
active Caspase-8 (red line).
Caspase-3 Expression in Jurkat Cells treated with
Anti-Fas antibodies. Human Jurkat leukemic T cells
were treated with anti-Fas antibodies to induce
apoptosis. Caspase-3 was detected using anti-hu-
man/mouse Active Caspase-3 polyclonal antibody
(Catalog # AF835) followed by Cy3-conjugated anti-rab-
bit secondary antibody (red). Cells were counterstained
with FluoroNissI™ Green (green).
Caspases & Regulators
CaspasePEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase InhibitorPan Specific
Multi-species Z-VAD-FMK FMK001 1 mg
Caspase InhibitorBiotinylatedPan Specific
Multi-species Z-VKD-biotin-FMK FMK011 500 µg
Caspase InhibitorPan Specific
Multi-species Q-VD, Non-omethylated OPH001 1 mg
Caspase Inhibitor ControlPan Specific
Multi-species Z-FA-FMK FMKC01 5 mg
Caspase InhibitorSampler PackPan Specific
Multi-species Z-VAD-FMK, Z-WEHD-FMK, Z-VDVAD-FMK, Z-DEVD-FMK,Z-YVAD-FMK, Z-VEID-FMK, Z-IETD-FMK, Z-LEHD-FMK,Z-AEVD-FMK, Z-LEED-FMK
FMKSP01 100 µg ofeach
Caspase-1ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Caspase-1 Human � Cell Culture Supernate 1.24 pg/mL 6.25-400 pg/mL DCA100 1 Kit
PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-1Colorimetric CaspaseActivity Assay
Multi-species WEHD BF14100 100 Tests
Caspase-1Fluorometric CaspaseActivity Assay
Multi-species WEHD BF12100 100 Tests
Caspase-1 Inhibitor Multi-species Z-WEHD-FMK FMK002 1 mg
Caspase-2RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Caspase-2Active
Human Solution with a carrier protein None E. coli 702-C2-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Caspase-2aa 414-428
Human/Mouse None Polyclonal Rabbit IgG WB AF826 80 µg
PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-2Colorimetric CaspaseActivity Assay
Multi-species VDVAD BF8100 100 Tests
Caspase-2Fluorometric CaspaseActivity Assay
Multi-species VDVAD BF5100 100 Tests
Caspase-2 Inhibitor Multi-species Z-VDVAD-FMK FMK003 1 mg
Caspase-3RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Caspase-3Active
Human Solution with a carrier protein None E. coli 707-C3-010 10 µg
Caspase-3Pro Form
Human Lyophilized without a carrier protein None E. coli 731-C3-005 5 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037320
Caspase-3 continuedANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Caspase-3aa 29-175
Human None 84803 Mouse IgG2B WB MAB707 100 µg
Caspase-3 Human/Mouse None Polyclonal Goat IgG IP, WB AF-605-NA 100 µg
Caspase-3Activeaa 163-175 (human)
Human/Mouse None Polyclonal Rabbit IgG IHC AF835 50 µg
Caspase-3Cleaved (Asp175)
Human/Mouse None 269518 Rabbit IgG IHC, WB MAB835 50 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Caspase-3Active
Human � Cell Extracts 0.1 ng/mL 0.31-20 ng/mL KM300 1 Kit
PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-3Colorimetric CaspaseActivity Assay
Multi-species DEVD BF3100 100 Tests
Caspase-3Fluorometric CaspaseActivity Assay
Multi-species DEVD BF1100 100 Tests
Caspase-3 Inhibitor Multi-species Z-DEVD-FMK FMK004 1 mg
Caspase-4PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-4 Inhibitor Multi-species Z-YVAD-FMK FMK005 1 mg
Caspase-6PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-6Colorimetric CaspaseActivity Assay
Multi-species VEID BF9100 100 Tests
Caspase-6Fluorometric CaspaseActivity Assay
Multi-species VEID BF6100 100 Tests
Caspase-6 Inhibitor Multi-species Z-VEID-FMK FMK006 1 mg
Caspase-7RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Caspase-7Active
Human Solution with a carrier protein None E. coli 823-C7-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Caspase-7 Human/Mouse None MCH3101.62 Mouse IgG1 WB MAB823 100 µg
Caspase-7aa 271-285 (human)
Human/Mouse None Polyclonal Rabbit IgG WB AF823 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 21
For antibody application key, see page 120.
Caspases & Regulators
Caspase-8RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Caspase-8Active
Human Solution with a carrier protein None E. coli 705-C8-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Caspase-8aa 234-391
Human None 84131 Mouse IgG2A WB MAB704 100 µg
Caspase-8 Human/Mouse None Polyclonal Rabbit IgG WB AF1650 100 µg
Caspase-8 Human/Mouse None Polyclonal Goat IgG WB AF705 100 µg
PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-8Colorimetric CaspaseActivity Assay
Multi-species IETD BF4100 100 Tests
Caspase-8Fluorometric CaspaseActivity Assay
Multi-species IETD BF2100 100 Tests
Caspase-8 Inhibitor Multi-species Z-IETD-FMK FMK007 1 mg
Caspase-9ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Caspase-9aa 1-134
Human None LAP6 Mouse IgG1 ELISA(Cap), IHC, WB MAB8301 100 µg
Caspase-9aa 139-330
Human None Polyclonal Goat IgG IHC, WB AF8301 100 µg
PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-9Colorimetric CaspaseActivity Assay
Multi-species LEHD BF10100 100 Tests
Caspase-9Fluorometric CaspaseActivity Assay
Multi-species LEHD BF7100 100 Tests
Caspase-9 Inhibitor Multi-species Z-LEHD-FMK FMK008 1 mg
Caspase-10RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Caspase-10Active Isoform a
Human Solution without a carrier protein None E. coli 834-CP-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Caspase-10Isoform a
Human None 63131 Mouse IgG2B WB MAB834 100 µg
Caspase-10Isoform baa 487-504
Human None Polyclonal Rabbit IgG WB AF834 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037322
Caspase-10 continuedPEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-10Colorimetric CaspaseActivity Assay
Multi-species WEHD BF18100 100 Tests
Caspase-10Fluorometric CaspaseActivity Assay
Multi-species AEVD BF16100 100 Tests
Caspase-10 Inhibitor Multi-species Z-AEVD-FMK FMK009 1 mg
Caspase-12ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Caspase-12 Mouse/Rat None Polyclonal Rabbit IgG WB AF1456 100 µg
Caspase-13PEPTIDES & PEPTIDE-BASED ASSAYS
PRODUCT SPECIES SEQUENCE CATALOG # SIZE
Caspase-13 Inhibitor Multi-species Z-LEED-FMK FMK010 1 mg
FLIPRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
FLIPWestern Blot Standard
Human Lyophilized without a carrier protein None E. coli WBC001 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
FLIPaa 432-450
Human/Mouse None Polyclonal Rabbit IgG WB AF821 100 µg
NALP2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
NALP2aa 1-208
Human None Polyclonal Sheep IgG WB AF4684 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 23
For antibody application key, see page 120.
Caspases & Regulators
Inhibitors of Apoptosis (IAPs) & Regulators
R&D Systems Tools for Cell Biology Research™
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037324
As their name implies, a primary role for members of the Inhibitors
of Apoptosis (IAP) family lies in their ability to suppress apoptosis.
This occurs through the inhibition of pro-apoptotic members of
the Caspase family. Some IAPs also act as ubiquitin ligases, target-
ing specific proteins for proteasomal degradation. IAP proteins are
characterized structurally by the presence of at least one BIR
(Baculoviral IAP Repeat) domain. Binding of the BIR domain of IAPs
to active caspases inhibits caspase activity. Eight IAPs have been
identified in humans including Apollon/BRUCE, cIAP-1, cIAP-2, ILP-2,
Livin, NAIP, Survivin, and XIAP. The activities of IAPs are themselves
suppressed by mitochondrial proteins released into the cytosol dur-
ing apoptosis, such as HTRA2/Omi and Smac/Diablo. These proteins
bind to the BIR domain of IAPs and reduce their ability to interact
with caspases.
For more information on IAP-related products, please visit our website
at www.RnDSystems.com/go/IAPs
The IAP Family of Proteins. Members of the Inhibitors of Apoptosis (IAP) family of proteins contain one or more baculoviral IAP repeat (BIR) domain through which they can di-
rectly bind to Caspase-3, -7, or -9 to inhibit their activity. Some IAPs also contain domains that are characteristic of enzymes involved in ubiquitin-mediated proteasomal
degradation, such as the UBC domain found in E2 ubiquitin-conjugating enzymes, or the RING finger domain found in many E3 ubiquitin ligases. In addition, cIAP-1 and cIAP-2,
like Pro-caspase-2 and Pro-caspase-9, contain a caspase-recruitment domain (CARD).
Inhibition of Caspase Activity by XIAP. Recombinant
human Caspase-3 (Catalog # 707-C3; purple line),
Caspase-7 (Catalog # 823-C7; gray line), or Cytochrome c
and dATP-activated Jurkat cell extracts (green line) were
mixed with increasing concentrations of recombinant
human XIAP (Catalog # 822-XF) prior to the addition of
the caspase peptide substrate, DEVD-afc. DEVD-afc
cleavage was monitored in a fluorescence plate reader.
Survivin in Human THP-1 and HeLa Cell Lysates. Hu-
man THP-1 monocytic leukemia cells and human HeLa
cells were either untreated (-) or treated (+) with
200 ng/mL nocodazole for 16 hours. Cells were lysed
and Survivin was assayed using the human Survivin
DuoSet® IC ELISA (Catalog # DYC647). Similar results
were obtained by Western blot analysis using anti-hu-
man Survivin polyclonal antibody (Catalog # AF886).
cIAP-1 in Human Lymphoma Tissue. cIAP-1 was de-
tected in paraffin-embedded human lymphoma tissue
sections using anti-human/mouse cIAP-1 polyclonal
antibody (Catalog # AF818). Tissues were stained using
anti-rabbit HRP-DAB Cell and Tissue Staining Kit
(Catalog # CTS005; brown) and counterstained with
hematoxylin (blue).
cIAPANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
cIAPPan Specific
Human/Mouse None 315301 Mouse IgG2A WB MAB3400 100 µg
cIAP-1/HIAP-2RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
cIAP-1/HIAP-2 Human Solution without a carrier protein None E. coli 818-IA-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
cIAP-1/HIAP-2 Human None Polyclonal Goat IgG IHC, WB AF8181 100 µg
cIAP-1/HIAP-2aa 527-546 (human)
Human/Mouse None Polyclonal Rabbit IgG WB AF818 100 µg
cIAP-2/HIAP-1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
cIAP-2/HIAP-1 Human Solution without a carrier protein None E. coli 817-P2-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
cIAP-2/HIAP-1 Human None Polyclonal Goat IgG IHC, WB AF8171 100 µg
cIAP-2/HIAP-1 Human None 315304 Mouse IgG1 IHC, WB MAB817 100 µg
HTRA2/OmiRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
HTRA2/Omi Human Solution without a carrier protein None E. coli 1458-HT-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HTRA2/Omi Human None 229926 Mouse IgG2B IHC, WB MAB1458 100 µg
HTRA2/Omi Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1458 100 µg
LivinRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Livin� Isoform
Human Solution without a carrier protein None E. coli 1161-LV-100 100 µg
Livin� Isoform
Human Solution without a carrier protein None E. coli 787-LV-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Livin Human None Polyclonal Goat IgG WB AF1161 100 µg
Livin Human Biotin Polyclonal Goat IgG WB BAF1161 50 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 25
For antibody application key, see page 120.
Inhibitors of Apoptosis (IAPs) & Regulators
Livin continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
LivinTotal Protein
Human 31.2 - 2000 pg/mL DYC1161-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
NAIPANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZ
NAIPaa 503-520
Human None Polyclonal Rabbit IgG WB AF829 100 µg
SMAC/DiabloRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
SMAC/Diablo Human Solution without a carrier protein None E. coli 789-SM-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SMAC/Diablo Human None Polyclonal Rabbit IgG WB AF789 100 µg
SMAC/Diablo Human None Polyclonal Goat IgG IHC, WB AF7891 100 µg
SMAC/Diablo Human Biotin Polyclonal Goat IgG WB BAF789 50 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
SMAC/DiabloTotal Protein
Human 62.5 - 4000 pg/mL DYC789-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
SurvivinRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
SurvivinWestern Blot Standard
Human Lyophilized without a carrier protein None N/A WBC010B 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Survivin Human None Polyclonal Goat IgG FC, WB AF6471 100 µg
Survivin Human None Polyclonal Rabbit IgG IHC, WB AF886 100 µg
Survivin Human Biotin Polyclonal Goat IgG FC, WB BAF6471 50 µg
Survivin Human Fluorescein 91630 Mouse IgG1 FC IC6472F 100 Tests
Survivin Human PE 91630 Mouse IgG1 FC IC6472P 100 Tests
Survivin Human None 91630 Mouse IgG1 FC MAB886 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037326
Survivin continuedELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Survivin Human � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
� Urine
9.96 pg/mL 31.2-2000 pg/mL DSV00 1 Kit
SurvivinTotal ProteinSurveyor IC ELISA
Human � Cell Lysate N/A 62.5-4000 pg/mL SUV647 1 Kit
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
SurvivinTotal Protein
Human 62.5 - 4000 pg/mL DYC647-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Survivin Human RDP-204-025 25 Tests
XIAPRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
XIAPBIR2 Domain
Human Solution without a carrier protein None E. coli 786-XB-050 50 µg
XIAPBIR3 Domain
Human Solution without a carrier protein None E. coli 895-XB-050 50 µg
XIAPFull Length
Human Solution without a carrier protein None E. coli 822-XF-050 50 µg
XIAPWestern Blot Standard
Human Solution without a carrier protein None E. coli WBC002 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
XIAP Human None 117318 Mouse IgG1 B/N, IHC MAB8221 100 µg
XIAP Human/Mouse None 117320 Mouse IgG2A WB MAB822 100 µg
XIAP Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF8221 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
XIAPTotal Protein
Human 312 - 20000 pg/mL DYC822-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 27
For antibody application key, see page 120.
Inhibitors of Apoptosis (IAPs) & Regulators
R&D Systems Tools for Cell Biology Research™
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037328
The Tumor Necrosis Factor (TNF) superfamily is a group of proteins
with important functions in immunity, inflammation, cell prolifera-
tion, differentiation, and apoptosis. Most members of the TNF
superfamily are type II transmembrane proteins. The extrinsic path-
way of caspase activation is mediated by death receptors
belonging to the TNF superfamily of receptors, including TNF RI,
Fas, DR3, TRAIL R1/DR4, and TRAIL R2/DR5. Ligand binding to
these receptors triggers the recruitment of adaptor proteins which
subsequently recruit pro-caspases, resulting in their activation. For
example, TNF-� binds to TNF RI and recruits the adaptor proteins,
TRADD and FADD leading to the subsequent formation of two sig-
naling complexes. One complex contains Pro-caspase-8 and
Pro-caspase-10 and culminates in the activation of the caspase cas-
cade leading to apoptosis. The second complex, containing TRADD
and TRAF-2, promotes the activation of the NF�B and MAPK path-
ways. Other members of the TNF superfamily are involved in
making specific cells more susceptible to death receptor-mediated
cell death, or in preventing apoptotic signaling.
For more information on TNF superfamily products, please visit our
website at www.RnDSystems.com/go/TNFSF
Death Receptors and Adaptor Proteins Involved in Apoptotic Signaling. Receptors of the TNF superfamily, including TNF RI, Fas, TRAIL R1 and R2, and DR3 activate
apoptotic signaling cascades upon ligand binding by recruiting adaptor proteins containing death domains (DD), such as TRADD and FADD. These adaptor proteins recruit
Pro-caspase-8, and -10 through their shared death effector domains (DED).
Fas Ligand-induced Apoptosis of Human Jurkat Cells
& Antibody Neutralization. The ability of recombinant
human Fas Ligand (Catalog # 126-FL) to induce
apoptosis was determined by measuring 3H-thymidine
incorporation into the DNA of human Jurkat T cells incu-
bated with increasing concentrations of Fas Ligand (blue
line). This effect was neutralized by incubating the Fas
Ligand (2 ng/mL) with increasing concentrations of
anti-human Fas Ligand polyclonal antibody (Catalog #
AF126) prior to its addition to Jurkat cells (orange line).
Intracellular Detection of TNF-� in EL-4 Cells by Flow
Cytometry. EL-4 mouse lymphoma cells were stained
with PE-conjugated anti-mouse TNF-� monoclonal anti-
body (Catalog # IC410P; filled histogram) or with
PE-conjugated rat IgG isotype control antibody (Catalog
# IC005P; open histogram)
TRAIL-induced Cytoxicity in Mouse L929 Cells. The
cytotoxicity of recombinant human TRAIL (Catalog #
375-TL) was determined by incubating mouse L929
fibroblast cells with increasing concentrations of the
cross-linked protein in the presence of the metabolic in-
hibitor actinomycin D. The cytotoxic effect induced by
TRAIL (20 ng/mL) was neutralized by incubating the
protein with increasing concentrations of anti-human
TRAIL/TNFSF10 polyclonal antibody (Catalog # AF375)
prior to its addition to L929 cells (inset).
TNF Superfamily
Extrinsic Pathway of Caspase Activation
4-1BB/TNFRSF9RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
4-1BB/TNFRSF9/Fc Chimera Human Lyophilized without a carrier protein None NS0 838-4B-100 100 µg
4-1BB/TNFRSF9/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 937-4B-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
4-1BB/TNFRSF9 Human None Polyclonal Goat IgG ELISA(Cap), FA, FC,IHC, WB
AF838 100 µg
4-1BB/TNFRSF9 Human Biotin Polyclonal Goat IgG ELISA(Det), FC, IHC,WB
BAF838 50 µg
4-1BB/TNFRSF9 Human PE 145501 Mouse IgG2B FC FAB838P 100 Tests
4-1BB/TNFRSF9 Human None 145501 Mouse IgG2B FC, WB MAB838 500 µg
4-1BB/TNFRSF9 Mouse None Polyclonal Goat IgG FA, FC, WB AF937 100 µg
4-1BB/TNFRSF9 Mouse Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF937 50 µg
4-1BB/TNFRSF9 Mouse None 158332 Rat IgG2A ELISA(Cap), WB MAB937 500 µg
4-1BB/TNFRSF9 Mouse None 158321 Rat IgG2A FA MAB9371 500 µg
4-1BB/TNFRSF9 Mouse None 158306 Rat IgG2B B/N, FC, WB MAB9372 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
4-1BB/TNFRSF9 Human 31.2 - 2000 pg/mL DY838 15 Plates
4-1BB/TNFRSF9 Mouse 31.2 - 2000 pg/mL DY937 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
4-1BB Ligand/TNFSF9RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
4-1BB Ligand/TNFSF9 Human Lyophilized with a carrier protein None E. coli 2295-4L-025 25 µg
4-1BB Ligand/TNFSF9 Mouse Lyophilized with a carrier protein None NS0 1246-4L-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
4-1BB Ligand/TNFSF9 Human PE 282220 Mouse IgG2B FC FAB2295P 100 Tests
4-1BB Ligand/TNFSF9 Human None 282220 Mouse IgG2B FC, WB MAB2295 100 µg
4-1BB Ligand/TNFSF9aa 71-254
Human None Polyclonal Goat IgG FC, WB AF2295 100 µg
4-1BB Ligand/TNFSF9aa 71-254
Human Biotin Polyclonal Goat IgG FC, WB BAF2295 50 µg
4-1BB Ligand/TNFSF9 Mouse None Polyclonal Goat IgG WB AF1246 100 µg
4-1BB Ligand/TNFSF9 Mouse Biotin Polyclonal Goat IgG WB BAF1246 50 µg
4-1BB Ligand/TNFSF9 Mouse APC 203942 Rat IgG2B FC FAB1246A 100 Tests
4-1BB Ligand/TNFSF9 Mouse Fluorescein 203942 Rat IgG2B FC FAB1246F 100 Tests
4-1BB Ligand/TNFSF9 Mouse PE 203942 Rat IgG2B FC FAB1246P 100 Tests
4-1BB Ligand/TNFSF9 Mouse None 203942 Rat IgG2B FC, WB MAB1246 500 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 29
For antibody application key, see page 120.
TNF Superfamily
APRIL/TNFSF13RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
APRIL/TNFSF13 Human Lyophilized with a carrier protein None NS0 884-AP-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
APRIL/TNFSF13 Human None Polyclonal Goat IgG FC, WB AF884 100 µg
APRIL/TNFSF13 Human Biotin Polyclonal Goat IgG FC, WB BAF884 50 µg
APRIL/TNFSF13 Human None 101115 Mouse IgG2A WB MAB884 500 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
APRIL/TNFSF13 Human RDP-127-025 25 Tests
BAFF/TNFSF13BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
BAFF/TNFSF13B Human Lyophilized with a carrier protein None NS0 2149-BF-010 10 µg
BAFF/TNFSF13B Mouse Lyophilized with a carrier protein None NS0 2106-BF-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BAFF/TNFSF13B Human None Polyclonal Goat IgG IHC, WB AF124 100 µg
BAFF/TNFSF13B Human Biotin Polyclonal Goat IgG WB BAF124 50 µg
BAFF/TNFSF13B Human Fluorescein 137314 Mouse IgG1 FC IC1241F 100 Tests
BAFF/TNFSF13B Human PE 137314 Mouse IgG1 FC IC1241P 100 Tests
BAFF/TNFSF13B Human None 148725 Mouse IgG2B B/N MAB124 500 µg
BAFF/TNFSF13B Human None 137314 Mouse IgG1 FC, WB MAB1241 500 µg
BAFF/TNFSF13B Mouse None Polyclonal Goat IgG B/N, WB AF2106 100 µg
BAFF/TNFSF13B Mouse Biotin Polyclonal Goat IgG IHC, WB BAF2106 50 µg
BAFF/TNFSF13B Mouse PE 121808 Rat IgG2A FC IC1357P 100 Tests
BAFF/TNFSF13B Mouse None 121808 Rat IgG2A FC, WB MAB1357 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
BAFF/TNFSF13B Human � Serum (S)
� EDTA and Heparin Plasma (P)
� Cell Culture Supernate (C)
11.9 pg/mL (S P)6.67 pg/mL (C)
62.5-4000 pg/mL (S P)31.2-2000 pg/mL (C)
DBLYS0 1 Kit
BAFF/TNFSF13B Mouse � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
7.8 pg/mL 46.9-3000 pg/mL MBLYS0 1 Kit
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
BAFF/TNFSF13B Human RDP-124-025 25 Tests
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037330
BAFF R/TNFRSF13CRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
BAFF R/TNFRSF13C/Fc Chimera
Human Lyophilized without a carrier protein None NS0 1162-BR-050 50 µg
BAFF R/TNFRSF13C/Fc Chimera
Mouse Lyophilized without a carrier protein None NS0 1357-BR-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BAFF R/TNFRSF13C Human None Polyclonal Goat IgG B/N, FC, WB AF1162 100 µg
BAFF R/TNFRSF13C Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), WB AF1357 100 µg
BAFF R/TNFRSF13C Mouse Biotin Polyclonal Goat IgG WB BAF1357 50 µg
BAFF R/TNFRSF13C Mouse Fluorescein 204406 Rat IgG2A FC FAB1755F 100 Tests
BAFF R/TNFRSF13C Mouse None 204406 Rat IgG2A FC MAB1755 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
BAFF R/TNFRSF13C Mouse 78.1 - 5000 pg/mL DY1357 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
BCMA/TNFRSF17RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
BCMA/TNFRSF17/Fc Chimera
Human Lyophilized without a carrier protein None NS0 193-BC-050 50 µg
BCMA/TNFRSF17/Fc Chimera
Mouse Lyophilized without a carrier protein None NS0 593-BC-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BCMA/TNFRSF17 Human None Polyclonal Goat IgG B/N, ELISA(Cap), FC,WB
AF193 100 µg
BCMA/TNFRSF17 Human Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF193 50 µg
BCMA/TNFRSF17 Human Fluorescein Polyclonal Goat IgG FC FAB193F 100 Tests
BCMA/TNFRSF17 Human PE Polyclonal Goat IgG FC FAB193P 100 Tests
BCMA/TNFRSF17 Human None 335004 Rat IgG2A B/N, WB MAB193 500 µg
BCMA/TNFRSF17 Mouse None Polyclonal Goat IgG B/N, WB AF593 100 µg
BCMA/TNFRSF17 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF593 50 µg
BCMA/TNFRSF17 Mouse Fluorescein 161616 Rat IgG1 FC FAB593F 100 Tests
BCMA/TNFRSF17 Mouse None 161616 Rat IgG1 FC MAB593 500 µg
BCMA/TNFRSF17 Mouse None 161608 Rat IgG2A B/N, ELISA(Cap), WB MAB5931 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
BCMA/TNFRSF17 Human 31.2 - 2000 pg/mL DY193 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
BCMA/TNFRSF17 Human RDP-140-025 25 Tests
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 31
For antibody application key, see page 120.
TNF Superfamily
CD27/TNFRSF7RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
CD27/TNFRSF7/Fc Chimera Human Lyophilized without a carrier protein None NS0 382-CD-100 100 µg
CD27/TNFRSF7/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 574-CD-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CD27/TNFRSF7 Human None Polyclonal Goat IgG B/N, FC, WB AF382 100 µg
CD27/TNFRSF7 Human Biotin Polyclonal Goat IgG FC, IHC, WB BAF382 50 µg
CD27/TNFRSF7 Human APC 57703 Mouse IgG1 FC FAB382A 100 Tests
CD27/TNFRSF7 Human Fluorescein 57703 Mouse IgG1 FC FAB382F 100 Tests
CD27/TNFRSF7 Human PE 57703 Mouse IgG1 FC FAB382P 100 Tests
CD27/TNFRSF7 Human None 57703 Mouse IgG1 B/N, FC, WB MAB382 500 µg
CD27/TNFRSF7 Mouse None Polyclonal Goat IgG FC, WB AF574 100 µg
CD27/TNFRSF7 Mouse Biotin Polyclonal Goat IgG WB BAF574 50 µg
CD27/TNFRSF7 Mouse Biotin 137925 Rat IgG2A ELISA(Det) BAM5741 250 µg
CD27/TNFRSF7 Mouse None 137910 Rat IgG2A FC, WB MAB574 100 µg
CD27/TNFRSF7 Mouse None 137915 Rat IgG2A ELISA(Cap), WB MAB5741 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
CD27/TNFRSF7 Mouse 62.5 - 4000 pg/mL DY574 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy pack . Standard DuoSet Kits are also available in 45 plate Economy Pack .
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
CD27/TNFRSF7 Human RDP-301-025 25 Tests
CD27 Ligand/TNFSF7RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
CD27 Ligand/TNFSF7 Mouse Lyophilized with a carrier protein None NS0 783-CL-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CD27 Ligand/TNFSF7 Human None 301731 Mouse IgG2B IHC, WB MAB2738 100 µg
CD27 Ligand/TNFSF7 Mouse None Polyclonal Goat IgG B/N, WB AF783 100 µg
CD27 Ligand/TNFSF7 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF783 50 µg
CD27 Ligand/TNFSF7 Mouse None 118510 Rat IgG1 B/N, ELISA(Cap) MAB783 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
CD27 Ligand/TNFSF7 Mouse 31.2 - 2000 pg/mL DY783 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
CD27 Ligand/TNFSF7 Mouse RDP-302-025 25 Tests
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037332
ss
CD30/TNFRSF8RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
CD30/TNFRSF8/Fc Chimera Human Lyophilized without a carrier protein None NS0 813-CD-100 100 µg
CD30/TNFRSF8/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 852-CD-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CD30/TNFRSF8 Human None Polyclonal Goat IgG FA, WB AF229 100 µg
CD30/TNFRSF8 Human Biotin Polyclonal Goat IgG WB BAF229 50 µg
CD30/TNFRSF8 Human Fluorescein 81337 Mouse IgG2B FC FAB229F 100 Tests
CD30/TNFRSF8 Human PE 81337 Mouse IgG2B FC FAB229P 100 Tests
CD30/TNFRSF8 Human None 81337 Mouse IgG2B FA, FC, WB MAB229 500 µg
CD30/TNFRSF8 Human None 81316 Mouse IgG1 WB MAB2291 500 µg
CD30/TNFRSF8 Mouse None Polyclonal Goat IgG FA, IHC, WB AF852 100 µg
CD30/TNFRSF8 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF852 50 µg
CD30/TNFRSF8 Mouse None 115705 Rat IgG2A ELISA(Cap), WB MAB8521 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
CD30/TNFRSF8 Mouse 15.6 - 1000 pg/mL DY852 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
CD30/TNFRSF8 Human RDP-303-025 25 Tests
CD30/TNFRSF8 Mouse/Rat RDP-304-025 25 Tests
CD30 Ligand/TNFSF8RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
CD30 Ligand/TNFSF8 Human Lyophilized without a carrier protein None NS0 1028-CL-050 50 µg
CD30 Ligand/TNFSF8 Mouse Lyophilized with a carrier protein None NS0 732-CL-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CD30 Ligand/TNFSF8 Human None Polyclonal Goat IgG FC, WB AF1028 100 µg
CD30 Ligand/TNFSF8 Human Biotin Polyclonal Goat IgG WB BAF1028 50 µg
CD30 Ligand/TNFSF8 Human APC 116614 Mouse IgG2B FC FAB1028A 100 Tests
CD30 Ligand/TNFSF8 Human PE 116614 Mouse IgG2B FC FAB1028P 100 Tests
CD30 Ligand/TNFSF8 Human None 116614 Mouse IgG2B B/N, FC MAB1028 500 µg
CD30 Ligand/TNFSF8 Human None 116615 Mouse IgG2A WB MAB774 500 µg
CD30 Ligand/TNFSF8 Mouse None Polyclonal Goat IgG B/N, WB AF732 100 µg
CD30 Ligand/TNFSF8 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF732 50 µg
CD30 Ligand/TNFSF8 Mouse None 82414 Rat IgG2A ELISA(Cap), WB MAB732 500 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 33
For antibody application key, see page 120.
TNF Superfamily
CD30 Ligand/TNFSF8 continuedDUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
CD30 Ligand/TNFSF8 Mouse 15.6 - 1000 pg/mL DY732 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
CD30 Ligand/TNFSF8 Human RDP-305-025 25 Tests
CD40/TNFRSF5RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
CD40/TNFRSF5/Fc Chimera Human Lyophilized without a carrier protein None NS0 1493-CD-050 50 µg
CD40/TNFRSF5/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 1215-CD-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CD40/TNFRSF5 Human None Polyclonal Goat IgG FA, WB AF632 100 µg
CD40/TNFRSF5 Human Biotin Polyclonal Goat IgG WB BAF632 50 µg
CD40/TNFRSF5 Human APC 82111 Mouse IgG2B FC FAB6321A 100 Tests
CD40/TNFRSF5 Human PE 82111 Mouse IgG2B FC FAB6321P 100 Tests
CD40/TNFRSF5 Human None 82105 Mouse IgG2B WB MAB632 500 µg
CD40/TNFRSF5 Human None 82111 Mouse IgG2B FA, FC, IHC MAB6321 500 µg
CD40/TNFRSF5 Human None 82102 Mouse IgG2B B/N, WB MAB6322 500 µg
CD40/TNFRSF5 Mouse None Polyclonal Goat IgG WB AF440 100 µg
CD40/TNFRSF5 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF440 50 µg
CD40/TNFRSF5 Mouse Biotin 1C10 Rat IgG2A IF, IP BAM440 250 µg
CD40/TNFRSF5 Mouse PE 65924 Rat IgG1 FC FAB4401P 100 Tests
CD40/TNFRSF5 Mouse None 1C10 Rat IgG2A FA, FC, IP MAB440 500 µg
CD40/TNFRSF5 Mouse None 65924 Rat IgG1 ELISA(Cap), FC, WB MAB4401 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
CD40/TNFRSF5 Mouse 15.6 - 1000 ng/mL DY097 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
CD40/TNFRSF5 Human RDP-306-025 25 Tests
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037334
CD40 Ligand/TNFSF5RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
CD40 Ligand/TNFSF5aa 108-261
Human Lyophilized with a carrier protein None E. coli 617-CL-050 50 µg
CD40 Ligand/TNFSF5Histidine-taggedaa 108-261
Human Lyophilized with a carrier protein None E. coli 2706-CL-025 25 µg
CD40 Ligand/TNFSF5aa 61-260
Mouse Lyophilized with a carrier protein None NS0 1163-CL-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CD40 Ligand/TNFSF5 Human None Polyclonal Goat IgG FC, IHC, WB AF617 100 µg
CD40 Ligand/TNFSF5 Human Biotin Polyclonal Goat IgG FC, WB BAF617 50 µg
CD40 Ligand/TNFSF5 Human APC 40804 Mouse IgG2B FC FAB617A 100 Tests
CD40 Ligand/TNFSF5 Human Fluorescein 40804 Mouse IgG2B FC FAB617F 100 Tests
CD40 Ligand/TNFSF5 Human PE 40804 Mouse IgG2B FC FAB617P 100 Tests
CD40 Ligand/TNFSF5 Human None 40804 Mouse IgG2B B/N, FC, WB MAB617 500 µg
CD40 Ligand/TNFSF5 Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,WB
AF1163 100 µg
CD40 Ligand/TNFSF5 Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1163 50 µg
CD40 Ligand/TNFSF5 Mouse APC 208109 Rat IgG2A FC FAB1163A 100 Tests
CD40 Ligand/TNFSF5 Mouse Fluorescein 208109 Rat IgG2A FC FAB1163F 100 Tests
CD40 Ligand/TNFSF5 Mouse PE 208109 Rat IgG2A FC FAB1163P 100 Tests
CD40 Ligand/TNFSF5 Mouse None 208109 Rat IgG2A B/N, FC MAB1163 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
CD40 Ligand/TNFSF5 Human � Serum
� Platelet-poor EDTA andHeparin Plasma
� Cell Culture Supernate
10.1 pg/mL 62.5-4000 pg/mL DCDL40 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
CD40 Ligand/TNFSF5 Human 15.6 - 1000 pg/mL DY617 15 Plates
CD40 Ligand/TNFSF5 Mouse 93.8 - 6000 pg/mL DY1163 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
CD40 Ligand/TNFSF5 Human RDP-307-025 25 Tests
CD40 Ligand/TNFSF5 Mouse/Rat RDP-308-025 25 Tests
CRMBRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
CRMB Viral Lyophilized with a carrier protein None NS0 4778-CR-010 10 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 35
For antibody application key, see page 120.
TNF Superfamily
DcR3/TNFRSF6BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
DcR3/TNFRSF6B/Fc Chimera Human Lyophilized without a carrier protein None Sf 21 142-DC-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
DcR3/TNFRSF6B Human None Polyclonal Goat IgG B/N, IHC, WB AF142 100 µg
DcR3/TNFRSF6B Human Biotin Polyclonal Goat IgG IHC, WB BAF142 50 µg
DcR3/TNFRSF6B Human None 229812 Mouse IgG1 B/N, WB MAB2135 500 µg
DcR3/TNFRSF6B Human None 229817 Mouse IgG1 ELISA(Cap), WB MAB21351 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
DcR3/TNFRSF6B Human 187 - 12000 pg/mL DY142 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
DcR3/TNFRSF6B Human RDP-115-025 25 Tests
DcTRAIL R1/TNFRSF23ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
DcTRAIL R1/TNFRSF23 Mouse None Polyclonal Goat IgG IHC, WB AF2378 100 µg
DcTRAIL R1/TNFRSF23 Mouse Biotin Polyclonal Goat IgG WB BAF2378 50 µg
DcTRAIL R1/TNFRSF23 Mouse Biotin 270012 Rat IgG2A IHC BAM2378 100 µg
DcTRAIL R1/TNFRSF23 Mouse None 270012 Rat IgG2A WB MAB2378 100 µg
DcTRAIL R1/TNFRSF23 Mouse None 270028 Rat IgG2A WB MAB23781 100 µg
DcTRAIL R2/TNFRSF22RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
DcTRAIL R2/TNFRSF22 Mouse Lyophilized with a carrier protein None NS0 1816-DT-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
DcTRAIL R2/TNFRSF22 Mouse None Polyclonal Goat IgG WB AF1816 100 µg
DcTRAIL R2/TNFRSF22 Mouse Biotin Polyclonal Goat IgG WB BAF1816 50 µg
DcTRAIL R2/TNFRSF22 Mouse None 263310 Rat IgG2B WB MAB1816 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037336
DR3/TNFRSF25RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
DR3/TNFRSF25/Fc Chimera Human Lyophilized without a carrier protein None NS0 943-D3-050 50 µg
DR3/TNFRSF25/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 2437-D3-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
DR3/TNFRSF25 Human None Polyclonal Goat IgG FC, WB AF943 100 µg
DR3/TNFRSF25 Human Biotin Polyclonal Goat IgG FC, WB BAF943 50 µg
DR3/TNFRSF25 Human None 59204 Mouse IgG1 WB MAB943 500 µg
DR3/TNFRSF25 Mouse None Polyclonal Goat IgG IHC, WB AF2437 100 µg
DR3/TNFRSF25 Mouse Biotin Polyclonal Goat IgG WB BAF2437 50 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
DR3/TNFRSF25 Human RDP-65-025 25 Tests
DR6/TNFRSF21RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
DR6/TNFRSF21/Fc Chimera Human Lyophilized without a carrier protein None Sf 21 144-DR-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
DR6/TNFRSF21 Human None Polyclonal Goat IgG ELISA(Cap), WB AF144 100 µg
DR6/TNFRSF21 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF144 50 µg
DR6/TNFRSF21 Human None 103402 Mouse IgG1 WB MAB1441 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
DR6/TNFRSF21 Human 62.5 - 4000 pg/mL DY144 15 Plates
* DuoSet IC Kits also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
DR6/TNFRSF21 Human/Mouse/Rat RDP-123-025 25 Tests
EDARECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
EDA Human Lyophilized with a carrier protein None NS0 3944-ED-010 10 µg
EDA Mouse Lyophilized with a carrier protein None NS0 191-ED-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
EDA Human Biotin 174817 Mouse IgG2B ELISA(Det) BAM9221 100 µg
EDA Human None 174806 Mouse IgG2B ELISA(Cap) MAB9222 500 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 37
For antibody application key, see page 120.
TNF Superfamily
EDA continuedDUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
EDA Human 93.75 - 6000 pg/mL DY922 15 Plates
* DuoSet IC Kits also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits also available in 45 plate Economy Packs.
EDA-A2RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
EDA-A2aa 179-389
Human Lyophilized with a carrier protein None NS0 922-ED-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
EDA-A2aa 179-389
Human None Polyclonal Goat IgG B/N, WB AF922 100 µg
EDA-A2aa 179-389
Human Biotin Polyclonal Goat IgG WB BAF922 50 µg
EDA-A2aa 179-389
Human None 174813 Mouse IgG1 WB MAB922 500 µg
EDARRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
EDAR/Fc Chimera Human Lyophilized without a carrier protein None NS0 157-ER-100 100 µg
EDAR/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 745-ED-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
EDAR Human None Polyclonal Goat IgG B/N, WB AF157 100 µg
EDAR Human Biotin Polyclonal Goat IgG WB BAF157 50 µg
EDAR Mouse None Polyclonal Goat IgG B/N, WB AF745 100 µg
EDAR Mouse Biotin Polyclonal Goat IgG WB BAF745 50 µg
EDAR Mouse None 132102 Rat IgG2A WB MAB745 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
EDAR Mouse 312.5 - 20000 pg/mL DY745 15 Plates
* DuoSet IC Kits also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits also available in 45 plate Economy Packs.
Fas/TNFRSF6RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Fas/TNFRSF6/Fc Chimera Feline Lyophilized without a carrier protein None NS0 2267-FA-050 50 µg
Fas/TNFRSF6/Fc Chimera Human Lyophilized with a carrier protein None NS0 326-FS-050 50 µg
Fas/TNFRSF6/Fc Chimera Mouse Lyophilized with a carrier protein None Sf 21 435-FA-050 50 µg
Fas/TNFRSF6/Fc Chimera Rat Lyophilized without a carrier protein None NS0 2159-FA-050 50 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037338
Fas/TNFRSF6 continuedANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Fas/TNFRSF6 Feline None Polyclonal Goat IgG FC, IHC, WB AF2267 100 µg
Fas/TNFRSF6 Feline Biotin Polyclonal Goat IgG FC, IHC, WB BAF2267 50 µg
Fas/TNFRSF6 Feline None 431014 Mouse IgG1 FC, WB MAB2267 100 µg
Fas/TNFRSF6aa 25-172
Feline None 431006 Mouse IgG1 WB MAB22671 100 µg
Fas/TNFRSF6 Human None Polyclonal Goat IgG WB AF326 100 µg
Fas/TNFRSF6 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF326 50 µg
Fas/TNFRSF6 Human Fluorescein DX2 Mouse IgG1 FC FAB142F 100 Tests
Fas/TNFRSF6 Human None DX2 Mouse IgG1 IHC, WB MAB142 500 µg
Fas/TNFRSF6 Human None 50830 Mouse IgG1 ELISA(Cap) MAB144 500 µg
Fas/TNFRSF6 Mouse None Polyclonal Goat IgG ELISA(Cap), FC, IHC,WB
AF435 100 µg
Fas/TNFRSF6 Mouse Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF435 50 µg
Fas/TNFRSF6 Mouse None 169732 Rat IgG2B FC MAB435 500 µg
Fas/TNFRSF6 Rat None Polyclonal Goat IgG FC, IHC, WB AF2159 100 µg
Fas/TNFRSF6 Rat Biotin Polyclonal Goat IgG WB BAF2159 50 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Fas/TNFRSF6 Human � Serum
� Citrate, EDTA, andHeparin Plasma
� Cell Culture Supernate
20 pg/mL 31.2-2000 pg/mL DFS00 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Fas/TNFRSF6 Human 62.5 - 4000 pg/mL DY326 15 Plates
Fas/TNFRSF6 Mouse 62.5 - 4000 pg/mL DY435 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Fas/TNFRSF6 Human/Mouse RDP-59-025 25 Tests
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 39
For antibody application key, see page 120.
TNF Superfamily
Fas Ligand/TNFSF6RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Fas Ligand/TNFSF6 Human Lyophilized with a carrier protein None CHO 126-FL-010 10 µg
Fas Ligand/TNFSF6 Mouse Lyophilized with a carrier protein None NS0 526-SA-050 50 µg
Fas Ligand/TNFSF6 Rat Lyophilized with a carrier protein None NS0 1614-FL-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Fas Ligand/TNFSF6 Human None Polyclonal Total Goat IgG B/N, WB AB126 1 mg
Fas Ligand/TNFSF6 Human None Polyclonal Goat IgG B/N, FC, IHC, WB AF126 100 µg
Fas Ligand/TNFSF6 Human Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF126 50 µg
Fas Ligand/TNFSF6 Human None 100419 Mouse IgG2B B/N, ELISA(Cap), FC MAB126 500 µg
Fas Ligand/TNFSF6 Human None 154922 Mouse IgG1 WB MAB1261 500 µg
Fas Ligand/TNFSF6 Mouse None Polyclonal Goat IgG B/N, WB AF526 100 µg
Fas Ligand/TNFSF6 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF526 50 µg
Fas Ligand/TNFSF6 Mouse None 101624 Rat IgG1 B/N, WB MAB526 500 µg
Fas Ligand/TNFSF6 Mouse None 101626 Rat IgG1 B/N, ELISA(Cap), WB MAB5262 500 µg
Fas Ligand/TNFSF6 Rat None Polyclonal Goat IgG IHC, WB AF1858 100 µg
Fas Ligand/TNFSF6 Rat Biotin Polyclonal Goat IgG WB BAF1889 50 µg
Fas Ligand/TNFSF6 Rat None 252029 Mouse IgG2B IHC, WB MAB1858 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Fas Ligand/TNFSF6 Human � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
� Cell Lysate
8.05 pg/mL 15.6-1000 pg/mL DFL00 1 Kit
Fas Ligand/TNFSF6 Mouse � Serum
� EDTA Plasma
� Cell Culture Supernate
3.9 pg/mL 31.2-2000 pg/mL MFL00 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Fas Ligand/TNFSF6 Human 31.2 - 2000 pg/mL DY126 15 Plates
Fas Ligand/TNFSF6 Mouse 15.6 - 1000 pg/mL DY526 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Fas Ligand/TNFSF6 Human/Mouse RDP-58-025 25 Tests
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037340
For antibody application key, see page 120.
GITR/TNFRSF18RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
GITR/TNFRSF18/Fc Chimera Human Lyophilized without a carrier protein None NS0 689-GR-100 100 µg
GITR/TNFRSF18/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 524-GR-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GITR/TNFRSF18 Human None Polyclonal Goat IgG B/N, FC, IHC, WB AF689 100 µg
GITR/TNFRSF18 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF689 50 µg
GITR/TNFRSF18 Human APC 110416 Mouse IgG1 FC FAB689A 100 Tests
GITR/TNFRSF18 Human Fluorescein 110416 Mouse IgG1 FC FAB689F 100 Tests
GITR/TNFRSF18 Human PE 110416 Mouse IgG1 FC FAB689P 100 Tests
GITR/TNFRSF18 Human None 110416 Mouse IgG1 B/N, ELISA(Cap), FC,WB
MAB689 500 µg
GITR/TNFRSF18 Mouse None Polyclonal Goat IgG WB AF524 100 µg
GITR/TNFRSF18 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF524 50 µg
GITR/TNFRSF18 Mouse Fluorescein 108619 Rat IgG2A FC FAB5241F 100 Tests
GITR/TNFRSF18 Mouse PE 108619 Rat IgG2A FC FAB5241P 100 Tests
GITR/TNFRSF18 Mouse None 108626 Rat IgG1 ELISA(Cap), WB MAB524 500 µg
GITR/TNFRSF18 Mouse None 108619 Rat IgG2A FC, WB MAB5241 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
GITR/TNFRSF18 Human 62.5 - 4000 pg/mL DY689 15 Plates
GITR/TNFRSF18 Mouse 62.5 - 4000 pg/mL DY524 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
GITR/TNFRSF18 Human RDP-128-025 25 Tests
GITR Ligand/TNFSF18RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
GITR Ligand/TNFSF18 Human Lyophilized with a carrier protein None Sf 21 694-GL-025 25 µg
GITR Ligand/TNFSF18 Mouse Lyophilized with a carrier protein None NS0 2177-GL-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GITR Ligand/TNFSF18 Human None Polyclonal Goat IgG B/N, FC, WB AF694 100 µg
GITR Ligand/TNFSF18 Human Biotin Polyclonal Goat IgG FC, WB BAF694 50 µg
GITR Ligand/TNFSF18 Human Biotin 109117 Mouse IgG1 ELISA(Det) BAM6943 250 µg
GITR Ligand/TNFSF18 Human APC 109101 Mouse IgG1 FC FAB6941A 100 Tests
GITR Ligand/TNFSF18 Human Fluorescein 109101 Mouse IgG1 FC FAB6941F 100 Tests
GITR Ligand/TNFSF18 Human PE 109101 Mouse IgG1 FC FAB6941P 100 Tests
GITR Ligand/TNFSF18 Human None 109101 Mouse IgG1 B/N, FC MAB6941 500 µg
GITR Ligand/TNFSF18 Human None 109114 Mouse IgG1 B/N, ELISA(Cap), FC MAB6942 500 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 41
TNF Superfamily
GITR Ligand/TNFSF18 continuedANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GITR Ligand/TNFSF18 Mouse None Polyclonal Goat IgG WB AF2177 100 µg
GITR Ligand/TNFSF18 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF2177 50 µg
GITR Ligand/TNFSF18 Mouse None 337122 Rat IgG2A B/N, ELISA(Cap), WB MAB2177 500 µg
GITR Ligand/TNFSF18 Mouse None 337112 Rat IgG2A WB MAB21771 100 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
GITR Ligand/TNFSF18 Human 125 - 8000 pg/mL DY694 15 Plates
GITR Ligand/TNFSF18 Mouse 31.25 - 2000 pg/mL DY2177 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
HVEM/TNFRSF14RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
HVEM/TNFRSF14/Fc Chimera Human Lyophilized with a carrier protein None NS0 356-HV-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HVEM/TNFRSF14 Human None Polyclonal Goat IgG WB AF356 100 µg
HVEM/TNFRSF14 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF356 50 µg
HVEM/TNFRSF14 Human APC 94801 Mouse IgG1 FC FAB356A 100 Tests
HVEM/TNFRSF14 Human None 94801 Mouse IgG1 ELISA(Cap), FC, WB MAB356 500 µg
HVEM/TNFRSF14 Human None 94804 Mouse IgG1 IHC, WB MAB3561 500 µg
HVEM/TNFRSF14 Mouse None Polyclonal Goat IgG WB AF2516 100 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
HVEM/TNFRSF14 Human 15.625 - 1000 pg/mL DY356 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
LIGHT/TNFSF14RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
LIGHT/TNFSF14 Human Lyophilized with a carrier protein None NS0 664-LI-025 25 µg
LIGHT/TNFSF14 Mouse Lyophilized with a carrier protein None NS0 1794-LT-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
LIGHT/TNFSF14 Human None Polyclonal Goat IgG B/N, WB AF664 100 µg
LIGHT/TNFSF14 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF664 50 µg
LIGHT/TNFSF14 Human APC 115520 Mouse IgG1 FC FAB664A 100 Tests
LIGHT/TNFSF14 Human PE 115520 Mouse IgG1 FC FAB664P 100 Tests
LIGHT/TNFSF14 Human None 115520 Mouse IgG1 ELISA(Cap), FC MAB664 500 µg
LIGHT/TNFSF14 Mouse None Polyclonal Goat IgG WB AF1794 100 µg
LIGHT/TNFSF14 Mouse Biotin Polyclonal Goat IgG WB BAF1794 50 µg
LIGHT/TNFSF14 Mouse None 261639 Rat IgG2A WB MAB1794 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037342
LIGHT/TNFSF14 continuedELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
LIGHT/TNFSF14 Human � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
� Saliva
16.5 pg/mL 31.2-2000 pg/mL DLIT00 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
LIGHT/TNFSF14 Human 62.5 - 4000 pg/mL DY664 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
LIGHT/TNFSF14 Human RDP-122-025 25 Tests
Lymphotoxin �
RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Lymphotoxin �1/�2 Human Lyophilized with a carrier protein None Sf 21 678-LY-010 10 µg
Lymphotoxin �2/�1 Human Lyophilized with a carrier protein None Sf 21 679-TX-010 10 µg
Lymphotoxin �2/�1 Mouse Lyophilized with a carrier protein None Sf 21 1008-LY-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Lymphotoxin �/� Human None 135125 Mouse IgG1 B/N MAB1370 500 µg
Lymphotoxin �/TNFSF3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Lymphotoxin �/TNFSF3 Human None 135105 Mouse IgG2B WB MAB1684 500 µg
Lymphotoxin � R/TNFRSF3RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Lymphotoxin � R/TNFRSF3/Fc Chimera
Human Lyophilized without a carrier protein None NS0 629-LR-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Lymphotoxin � R/TNFRSF3 Human None Polyclonal Goat IgG FA, FC, WB AF629 100 µg
Lymphotoxin � R/TNFRSF3 Human Biotin Polyclonal Goat IgG FC, WB BAF629 50 µg
Lymphotoxin � R/TNFRSF3 Human APC 71319 Mouse IgG1 FC FAB629A 100 Tests
Lymphotoxin � R/TNFRSF3 Human PE 71319 Mouse IgG1 FC FAB629P 100 Tests
Lymphotoxin � R/TNFRSF3 Human None 71319 Mouse IgG1 FC, WB MAB629 500 µg
Lymphotoxin � R/TNFRSF3 Mouse None Polyclonal Goat IgG FC, IHC, WB AF1008 100 µg
Lymphotoxin � R/TNFRSF3 Mouse Biotin Polyclonal Goat IgG FC, IHC, WB BAF1008 50 µg
Lymphotoxin � R/TNFRSF3 Mouse None 157105 Rat IgG2B WB MAB1008 500 µg
Lymphotoxin � R/TNFRSF3 Mouse None 157108 Rat IgG2A FC, WB MAB10081 500 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 43
For antibody application key, see page 120.
TNF Superfamily
NGF R/TNFRSF16RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
NGF R/TNFRSF16/Fc Chimera
Human Lyophilized with a carrier protein None Sf 21 367-NR-050 50 µg
NGF R/TNFRSF16/Fc Chimera
Mouse Lyophilized without a carrier protein None NS0 1157-NR-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
NGF R/TNFRSF16 Human None Polyclonal Goat IgG WB AF367 100 µg
NGF R/TNFRSF16 Human Biotin Polyclonal Goat IgG WB BAF367 50 µg
NGF R/TNFRSF16 Human None 74902 Mouse IgG1 IHC, WB MAB367 100 µg
NGF R/TNFRSF16 Mouse None Polyclonal Goat IgG IHC, WB AF1157 100 µg
NGF R/TNFRSF16 Mouse Biotin Polyclonal Goat IgG IHC, WB BAF1157 50 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
NGF R/TNFRSF16 Human RDP-112-025 25 Tests
NGF R/TNFRSF16 Mouse/Rat RDP-113-025 25 Tests
Osteoprotegerin/TNFRSF11BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Osteoprotegerin/TNFRSF11B
Human Lyophilized with a carrier protein None NS0 185-OS-025 25 µg
Osteoprotegerin/TNFRSF11B/Fc Chimera
Human Lyophilized with a carrier protein None Sf 21 805-OS-100 100 µg
Osteoprotegerin/TNFRSF11B/Fc Chimera
Mouse Lyophilized without a carrier protein None NS0 459-MO-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Osteoprotegerin/TNFRSF11B
Human None Polyclonal Goat IgG B/N, IHC, WB AF805 100 µg
Osteoprotegerin/TNFRSF11B
Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF805 50 µg
Osteoprotegerin/TNFRSF11B
Human None 69146 Mouse IgG1 B/N MAB805 500 µg
Osteoprotegerin/TNFRSF11B
Human None 69127 Mouse IgG2A ELISA(Cap) MAB8051 500 µg
Osteoprotegerin/TNFRSF11B
Mouse None Polyclonal Goat IgG B/N, WB AF459 100 µg
Osteoprotegerin/TNFRSF11B
Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF459 50 µg
Osteoprotegerin/TNFRSF11B
Mouse None 110517 Rat IgG2A WB MAB459 500 µg
Osteoprotegerin/TNFRSF11B
Mouse None 110511 Rat IgG2A ELISA(Cap), WB MAB4591 500 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037344
For antibody application key, see page 120.
Osteoprotegerin/TNFRSF11B continuedELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Osteoprotegerin/TNFRSF11B
Mouse � Serum
� EDTA Plasma
� Cell Culture Supernate
4.5 pg/mL 31.2-2000 pg/mL MOP00 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Osteoprotegerin/TNFRSF11B
Human 62.5 - 4000 pg/mL DY805 15 Plates
Osteoprotegerin/TNFRSF11B
Mouse 62.5 - 4000 pg/mL DY459 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Osteoprotegerin/TNFRSF11B Human/Mouse RDP-87-025 25 Tests
OX40/TNFRSF4RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
OX40/TNFRSF4/Fc Chimera Human Lyophilized without a carrier protein None NS0 3388-OX-050 50 µg
OX40/TNFRSF4/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 1256-OX-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
OX40/TNFRSF4 Human None Polyclonal Sheep IgG FC, WB AF3388 100 µg
OX40/TNFRSF4 Human Biotin Polyclonal Sheep IgG FC, WB BAF3388 50 µg
OX40/TNFRSF4 Human None 443318 Rat IgG2A FC, WB MAB3388 100 µg
OX40/TNFRSF4 Mouse None Polyclonal Goat IgG FA, WB AF1256 100 µg
OX40/TNFRSF4 Mouse Biotin Polyclonal Goat IgG WB BAF1256 50 µg
OX40 Ligand/TNFSF4RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
OX40 Ligand/TNFSF4 Human Lyophilized without a carrier protein None NS0 1054-OX-010 10 µg
OX40 Ligand/TNFSF4 Mouse Lyophilized with a carrier protein None NS0 1236-OX-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
OX40 Ligand/TNFSF4 Human None Polyclonal Goat IgG B/N, FC, WB AF1054 100 µg
OX40 Ligand/TNFSF4 Human Biotin Polyclonal Goat IgG WB BAF1054 50 µg
OX40 Ligand/TNFSF4 Human None 159408 Mouse IgG1 WB MAB1054 500 µg
OX40 Ligand/TNFSF4 Human None 159403 Mouse IgG1 B/N, FC, IHC MAB10541 500 µg
OX40 Ligand/TNFSF4 Mouse None Polyclonal Goat IgG B/N, IHC, WB AF1236 100 µg
OX40 Ligand/TNFSF4 Mouse Biotin Polyclonal Goat IgG WB BAF1236 50 µg
OX40 Ligand/TNFSF4 Mouse None 182601 Rat IgG2A B/N, WB MAB1236 500 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 45
TNF Superfamily
RANK/TNFRSF11ARECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
RANK/TNFRSF11A/Fc Chimera Human Lyophilized without a carrier protein None NS0 683-RK-100 100 µg
RANK/TNFRSF11A/Fc Chimera Mouse Lyophilized without a carrier protein None NS0 692-RK-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RANK/TNFRSF11A Human None Polyclonal Goat IgG FA, FC, WB AF683 100 µg
RANK/TNFRSF11A Human Biotin Polyclonal Goat IgG ELISA(Det), FC, WB BAF683 50 µg
RANK/TNFRSF11A Human None 80704 Mouse IgG1 ELISA(Cap), FC, WB MAB683 500 µg
RANK/TNFRSF11A Human None 80707 Mouse IgG2A IHC, WB MAB6831 500 µg
RANK/TNFRSF11A Mouse None Polyclonal Goat IgG FA, IHC, WB AF692 100 µg
RANK/TNFRSF11A Mouse Biotin Polyclonal Goat IgG WB BAF692 50 µg
RANK/TNFRSF11A Mouse None 100024 Rat IgG2A WB MAB692 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
RANK/TNFRSF11A Human 62.5 - 4000 pg/mL DY683 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
RANK/TNFRSF11A Human RDP-67-025 25 Tests
RANK/TNFRSF11A Mouse RDP-72-025 25 Tests
RANK Ligand, see page 55.
RELT/TNFRSF19LRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
RELT/TNFRSF19L/Fc Chimera Human Solution without a carrier protein None NS0 1385-RT-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RELT/TNFRSF19L Human None Polyclonal Goat IgG WB AF1385 100 µg
RELT/TNFRSF19L Human Biotin Polyclonal Goat IgG WB BAF1385 50 µg
RELT/TNFRSF19L Human None 238104 Mouse IgG2B FC MAB1385 500 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037346
For antibody application key, see page 120.
TACI/TNFRSF13BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TACI/TNFRSF13B/Fc Chimera
Human Lyophilized without a carrier protein None NS0 174-TC-050 50 µg
TACI/TNFRSF13B/Fc Chimera
Mouse Lyophilized without a carrier protein None NS0 1041-TC-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TACI/TNFRSF13B Human None Polyclonal Goat IgG B/N, IHC, WB AF174 100 µg
TACI/TNFRSF13B Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF174 50 µg
TACI/TNFRSF13B Human APC 165604 Mouse IgG1 FC FAB1741A 100 Tests
TACI/TNFRSF13B Human PE 165604 Mouse IgG1 FC FAB1741P 100 Tests
TACI/TNFRSF13B Human None 165609 Mouse IgG1 B/N, IHC, WB MAB174 500 µg
TACI/TNFRSF13B Human None 165604 Mouse IgG1 B/N, FC MAB1741 500 µg
TACI/TNFRSF13B Mouse None Polyclonal Goat IgG B/N, FC, IHC, WB AF1041 100 µg
TACI/TNFRSF13B Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1041 50 µg
TACI/TNFRSF13B Mouse PE 166010 Rat IgG2A FC FAB1041P 100 Tests
TACI/TNFRSF13B Mouse None 166010 Rat IgG2A ELISA(Cap), FC, WB MAB1041 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TACI/TNFRSF13B Human 93.8 - 6000 pg/mL DY174 15 Plates
TACI/TNFRSF13B Mouse 46.9 - 3000 pg/mL DY1041 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TACI/TNFRSF13B Human RDP-142-025 25 Tests
TL1A/TNFSF15RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TL1A/TNFSF15 Human Lyophilized with a carrier protein None E. coli 1319-TL-010 10 µg
TL1A/TNFSF15 Mouse Lyophilized with a carrier protein None E. coli 1896-TL-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TL1A/TNFSF15 Human None Polyclonal Goat IgG WB AF744 100 µg
TL1A/TNFSF15 Human Biotin Polyclonal Goat IgG WB BAF744 50 µg
TL1A/TNFSF15 Human/Mouse None 293327 Rat IgG1 WB MAB7441 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 47
TNF Superfamily
TNF-�/TNFSF1ARECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TNF-�/TNFSF1A Bovine Lyophilized with a carrier protein None E. coli 2279-BT-025 25 µg
TNF-�/TNFSF1A Canine Lyophilized with a carrier protein None E. coli 1507-CT-025 25 µg
TNF-�/TNFSF1A Cotton Rat Lyophilized with a carrier protein None E. coli 1011-CR-025 25 µg
TNF-�/TNFSF1A Equine Lyophilized with a carrier protein None E. coli 1814-ET-025 25 µg
TNF-�/TNFSF1A Feline Lyophilized with a carrier protein None E. coli 2586-FT-025 25 µg
TNF-�/TNFSF1A Human Lyophilized with a carrier protein None E. coli 210-TA-010 10 µg
TNF-�/TNFSF1A Human Lyophilized with a carrier protein None E. coli 210-TA-050 50 µg
TNF-�/TNFSF1APro Form
Human Lyophilized without a carrier protein None E. coli 1012-PS-010 10 µg
TNF-�/TNFSF1A Mouse Lyophilized with a carrier protein None E. coli 410-MT-010 10 µg
TNF-�/TNFSF1A Mouse Lyophilized with a carrier protein None E. coli 410-MT-050 50 µg
TNF-�/TNFSF1AN-Terminal Truncated Form
Mouse Lyophilized with a carrier protein None E. coli 410-TRNC-010 10 µg
TNF-�/TNFSF1AN-Terminal Truncated Form
Mouse Lyophilized with a carrier protein None E. coli 410-TRNC-050 50 µg
TNF-�/TNFSF1A Porcine Lyophilized with a carrier protein None E. coli 690-PT-025 25 µg
TNF-�/TNFSF1A Primate Lyophilized with a carrier protein None E. coli 1070-RM-025 25 µg
TNF-�/TNFSF1A Rat Lyophilized with a carrier protein None E. coli 510-RT-010 10 µg
TNF-�/TNFSF1A Rat Lyophilized with a carrier protein None E. coli 510-RT-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TNF-�/TNFSF1A Bovine None Polyclonal Goat IgG IHC, WB AF2279 100 µg
TNF-�/TNFSF1A Bovine Biotin Polyclonal Goat IgG IHC, WB BAF2279 50 µg
TNF-�/TNFSF1A Canine None Polyclonal Goat IgG B/N, IHC, WB AF1507 100 µg
TNF-�/TNFSF1A Canine Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1507 50 µg
TNF-�/TNFSF1A Canine None 236812 Mouse IgG1 B/N, ELISA(Cap), IHC MAB1507 500 µg
TNF-�/TNFSF1A Cotton Rat None Polyclonal Goat IgG B/N, WB AF1011 100 µg
TNF-�/TNFSF1A Cotton Rat Biotin Polyclonal Goat IgG WB BAF1011 50 µg
TNF-�/TNFSF1A Cotton Rat None 159811 Mouse IgG2B B/N, WB MAB1011 500 µg
TNF-�/TNFSF1A Cotton Rat None 159813 Mouse IgG1 B/N, WB MAB10111 500 µg
TNF-�/TNFSF1A Equine None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,WB
AF1814 100 µg
TNF-�/TNFSF1A Human None Polyclonal Goat IgG B/N, IHC, WB AF-210-NA 100 µg
TNF-�/TNFSF1A Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF210 50 µg
TNF-�/TNFSF1A Human Fluorescein 6402 Mouse IgG1 FC IC210F 100 Tests
TNF-�/TNFSF1A Human PE 6402 Mouse IgG1 FC IC210P 100 Tests
TNF-�/TNFSF1A Human None 1825 Mouse IgG1 B/N MAB210 500 µg
TNF-�/TNFSF1A Human None 6401 Mouse IgG1 B/N, FC MAB2101 500 µg
TNF-�/TNFSF1A Human None 28401 Mouse IgG1 B/N, ELISA(Cap), IHC,WB
MAB610 500 µg
TNF-�/TNFSF1AMembrane Form
Human Fluorescein 6401 Mouse IgG1 FC FAB210F 50 Tests
TNF-�/TNFSF1A Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), FC,IHC, WB
AF-410-NA 100 µg
TNF-�/TNFSF1A Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF410 50 µg
TNF-�/TNFSF1A Mouse Fluorescein MP6-XT22 Rat IgG1 FC IC410F 100 Tests
TNF-�/TNFSF1A Mouse PE MP6-XT22 Rat IgG1 FC IC410P 100 Tests
TNF-�/TNFSF1A Mouse None TN319 Hamster IgG WB MAB410 500 µg
TNF-�/TNFSF1A Mouse None MP6-XT22 Rat IgG1 B/N MAB4101 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037348
For antibody application key, see page 120.
TNF-�/TNFSF1A continuedANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TNF-�/TNFSF1A Porcine None Polyclonal Goat IgG B/N, IHC, WB AF690 100 µg
TNF-�/TNFSF1A Porcine Biotin Polyclonal Goat IgG ELISA(Det), WB BAF690 50 µg
TNF-�/TNFSF1A Porcine None 77938 Mouse IgG1 WB MAB690 500 µg
TNF-�/TNFSF1A Porcine None 103314 Mouse IgG1 B/N MAB6901 500 µg
TNF-�/TNFSF1A Porcine None 103304 Mouse IgG1 ELISA(Cap) MAB6902 500 µg
TNF-�/TNFSF1A Porcine None 103302 Mouse IgG1 B/N, IHC, WB MAB6903 100 µg
TNF-�/TNFSF1A Primate None Polyclonal Goat IgG WB AF1070 100 µg
TNF-�/TNFSF1A Primate Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1070 50 µg
TNF-�/TNFSF1A Primate None 182303 Rat IgG2B WB MAB1070 500 µg
TNF-�/TNFSF1A Primate None 182309 Rat IgG1 B/N MAB10701 500 µg
TNF-�/TNFSF1A Primate None 182312 Rat IgG2A ELISA(Cap) MAB10702 500 µg
TNF-�/TNFSF1A Rat None Polyclonal Goat IgG B/N, IHC, WB AF-510-NA 100 µg
TNF-�/TNFSF1A Rat Biotin Polyclonal Goat IgG ELISA(Det), WB BAF510 50 µg
TNF-�/TNFSF1A Rat None 45418 Mouse IgG1 B/N, ELISA(Cap), WB MAB510 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
TNF-�/TNFSF1A Canine � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
4.2 pg/mL 7.8-500 pg/mL CATA00 1 Kit
TNF-�/TNFSF1A Human � Serum
� Citrate, EDTA, andHeparin Plasma
� Cell Culture Supernate
5.5 pg/mL 15.6-1000 pg/mL DTA00C 1 Kit
TNF-�/TNFSF1AChemiluminescent
Human � Serum
� Citrate, EDTA, andHeparin Plasma
� Cell Culture Supernate
0.736 pg/mL 2.2-7000 pg/mL QTA00B 1 Kit
TNF-�/TNFSF1AHigh Sensitivity
Human � Serum
� Citrate, EDTA, andHeparin Plasma
0.191 pg/mL 0.5-32 pg/mL HSTA00D 1 Kit
TNF-�/TNFSF1A2 Plate
Mouse � Serum
� Cell Culture Supernate
5.1 pg/mL 23.4-1500 pg/mL MTA00 1 Kit
TNF-�/TNFSF1A Porcine � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
5 pg/mL 23.4-1500 pg/mL PTA00 1 Kit
TNF-�/TNFSF1A Primate � Serum
� EDTA Plasma
� Cell Culture Supernate
6.2 pg/mL 15.6-1000 pg/mL RHMTA0 1 Kit
TNF-�/TNFSF1A2 Plate
Rat � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
5 pg/mL 12.5-800 pg/mL RTA00 1 Kit
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 49
TNF Superfamily
TNF-�/TNFSF1A continuedDUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TNF-�/TNFSF1A Canine 15.6 - 1000 pg/mL DY1507 15 Plates
TNF-�/TNFSF1A Equine 31.2 - 2000 pg/mL DY1814 15 Plates
TNF-�/TNFSF1A Feline 15.625 - 1000 pg/mL DY2586 15 Plates
TNF-�/TNFSF1A Human 15.6 - 1000 pg/mL DY210 15 Plates
TNF-�/TNFSF1A Mouse 31.2 - 2000 pg/mL DY410 15 Plates
TNF-�/TNFSF1A Porcine 125 - 8000 pg/mL DY690 15 Plates
TNF-�/TNFSF1A Primate 31.2 - 2000 pg/mL DY1070 15 Plates
TNF-�/TNFSF1A Rat 62.5 - 4000 pg/mL DY510 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
ELISPOT KITS
ANALYTE SPECIES CATALOG # SIZE**
TNF-�/TNFSF1A Human EL210 1 Kit
TNF-�/TNFSF1A Mouse EL410 1 Kit
TNF-�/TNFSF1A Porcine EL690 1 Kit
TNF-�/TNFSF1A Primate EL1070 1 Kit
** ELISpot Kits are also available in 6 and 50 plate packs and development modules containing enough reagents for 5 plates, please inquire.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TNF-�/TNFSF1A Human RDP-10-025 25 Tests
TNF-�/TNFSF1A Mouse RDP-60-025 25 Tests
TNF-�/TNFSF1BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TNF-�/TNFSF1B Human Lyophilized with a carrier protein None E. coli 211-TB-010 10 µg
TNF-�/TNFSF1B Human Lyophilized with a carrier protein None E. coli 211-TB-050 50 µg
TNF-�/TNFSF1B Mouse Lyophilized with a carrier protein None NS0 749-TB-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TNF-�/TNFSF1B Human None Polyclonal Goat IgG B/N, WB AF-211-NA 100 µg
TNF-�/TNFSF1B Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF211 50 µg
TNF-�/TNFSF1B Human Fluorescein 5808 Mouse IgG1 FC IC2111F 100 Tests
TNF-�/TNFSF1B Human PE 5808 Mouse IgG1 FC IC2111P 100 Tests
TNF-�/TNFSF1B Human None 5802 Mouse IgG1 IHC MAB211 500 µg
TNF-�/TNFSF1B Human None 5807 Mouse IgG2A B/N, ELISA(Cap) MAB621 1 mg
TNF-�/TNFSF1B Mouse None Polyclonal Goat IgG WB AF749 100 µg
TNF-�/TNFSF1B Mouse Biotin Polyclonal Goat IgG IHC, WB BAF749 50 µg
TNF-�/TNFSF1B Mouse None 168717 Rat IgG2B WB MAB749 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
TNF-�/TNFSF1B Human � Cell Culture Supernate 7 pg/mL 31.2-2000 pg/mL DTB00 1 Kit
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037350
TNF-�/TNFSF1B continuedDUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TNF-�/TNFSF1B Human 31.2 - 2000 pg/mL DY211 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TNF-�/TNFSF1B Human RDP-22-025 25 Tests
TNF RI/TNFRSF1ARECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TNF RI/TNFRSF1A Canine Lyophilized with a carrier protein None E. coli 4017-TR-025 25 µg
TNF RI/TNFRSF1A Human Lyophilized with a carrier protein None E. coli 636-R1-025 25 µg
TNF RI/TNFRSF1A/Fc Chimera
Human Lyophilized with a carrier protein None NS0 372-RI-050 50 µg
TNF RI/TNFRSF1A Mouse Lyophilized with a carrier protein None E. coli 425-R1-050 50 µg
TNF RI/TNFRSF1A/Fc Chimera
Mouse Lyophilized with a carrier protein None NS0 430-RI-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TNF RI/TNFRSF1A Human None Polyclonal Goat IgG FA, FC, IHC, WB AF225 100 µg
TNF RI/TNFRSF1A Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF225 50 µg
TNF RI/TNFRSF1A Human Fluorescein 16803 Mouse IgG1 FC FAB225F 100 Tests
TNF RI/TNFRSF1A Human PE 16803 Mouse IgG1 FC FAB225P 100 Tests
TNF RI/TNFRSF1A Human None 16803 Mouse IgG1 B/N, FC, WB MAB225 500 µg
TNF RI/TNFRSF1A Human None 16805 Mouse IgG1 B/N, ELISA(Cap), WB MAB625 500 µg
TNF RI/TNFRSF1A Mouse None Polyclonal Goat IgG IHC, WB AF-425-PB 100 µg
TNF RI/TNFRSF1A Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF425 50 µg
TNF RI/TNFRSF1A Mouse None 47803 Rat IgG1 ELISA(Cap), WB MAB425 500 µg
TNF RI/TNFRSF1A Mouse None 55R170 Hamster IgG B/N, IP, WB MAB430 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
TNF RI/TNFRSF1A Human � Serum
� Citrate, EDTA, andHeparin Plasma
� Cell Culture Supernate
� Urine
1.2 pg/mL 7.8-500 pg/mL DRT100 1 Kit
TNF RI/TNFRSF1A Mouse � Serum
� Cell Culture Supernate
5 pg/mL 15.6-1000 pg/mL MRT10 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TNF RI/TNFRSF1A Human 12.5 - 800 pg/mL DY225 15 Plates
TNF RI/TNFRSF1A Mouse 7.81 - 500 pg/mL DY425 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 51
For antibody application key, see page 120.
TNF Superfamily
TNF RI/TNFRSF1A continuedPRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TNF RI/TNFRSF1A Human RDP-61-025 25 Tests
TNF RI/TNFRSF1A Mouse RDP-80-025 25 Tests
TNF RII/TNFRSF1BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TNF RII/TNFRSF1Baa 24-206
Human Lyophilized with a carrier protein None E. coli 1089-R2-025 25 µg
TNF RII/TNFRSF1B/Fc Chimera
Human Lyophilized without a carrier protein None NS0 726-R2-050 50 µg
TNF RII/TNFRSF1B Mouse Lyophilized with a carrier protein None E. coli 426-R2-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TNF RII/TNFRSF1B Human None Polyclonal Total Goat IgG IHC, WB AB-226-PB 1 mg
TNF RII/TNFRSF1B Human None Polyclonal Goat IgG B/N, FC, WB AF726 100 µg
TNF RII/TNFRSF1B Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF726 50 µg
TNF RII/TNFRSF1B Human Fluorescein 22235 Mouse IgG2A FC FAB226F 100 Tests
TNF RII/TNFRSF1B Human PE 22235 Mouse IgG2A FC FAB226P 100 Tests
TNF RII/TNFRSF1B Human None 22221 Mouse IgG2A B/N, IHC MAB226 500 µg
TNF RII/TNFRSF1B Human None 22235 Mouse IgG2A FC MAB2261 500 µg
TNF RII/TNFRSF1B Human None 22210 Mouse IgG1 B/N, ELISA(Cap), WB MAB726 500 µg
TNF RII/TNFRSF1B Mouse None Polyclonal Goat IgG IHC, WB AF-426-PB 100 µg
TNF RII/TNFRSF1B Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF426 50 µg
TNF RII/TNFRSF1B Mouse None TR7554 Hamster IgG ELISA(Cap), WB MAB426 500 µg
TNF RII/TNFRSF1B Mouse None TR7532 Hamster IgG WB MAB4261 500 µg
TNF RII/TNFRSF1B Mouse None TR75-89.29 Hamster IgG IP, WB MAB4262 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
TNF RII/TNFRSF1B Human � Serum
� Citrate, EDTA, andHeparin Plasma
� Cell Culture Supernate
� Urine
2.3 pg/mL 7.8-500 pg/mL DRT200 1 Kit
TNF RII/TNFRSF1B Mouse � Serum
� EDTA Plasma
� Cell Culture Supernate
5 pg/mL 15.6-1000 pg/mL MRT20 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TNF RII/TNFRSF1B Human 7.81 - 500 pg/mL DY726 15 Plates
TNF RII/TNFRSF1B Mouse 7.81 - 500 pg/mL DY426 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TNF RII/TNFRSF1B Human/Mouse RDP-62-025 25 Tests
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037352
For antibody application key, see page 120.
TRAIL/TNFSF10RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TRAIL/TNFSF10 Human Lyophilized with a carrier protein None NS0 375-TL-010 10 µg
TRAIL/TNFSF10 Human Lyophilized with a carrier protein None E. coli 375-TEC-010 10 µg
TRAIL/TNFSF10 Mouse Lyophilized with a carrier protein None E. coli 1121-TL-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAIL/TNFSF10 Human None Polyclonal Goat IgG B/N, IHC, WB AF375 100 µg
TRAIL/TNFSF10 Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF375 50 µg
TRAIL/TNFSF10 Human PE 75402 Mouse IgG1 FC FAB687P 100 Tests
TRAIL/TNFSF10 Human None 75411 Mouse IgG1 B/N, IHC MAB375 500 µg
TRAIL/TNFSF10 Human None 124723 Mouse IgG1 ELISA(Cap) MAB3751 500 µg
TRAIL/TNFSF10 Human None 75402 Mouse IgG1 FC, IHC, WB MAB687 500 µg
TRAIL/TNFSF10 Mouse None Polyclonal Goat IgG IHC, WB AF1121 100 µg
TRAIL/TNFSF10 Mouse Biotin Polyclonal Goat IgG IHC, WB BAF1121 50 µg
TRAIL/TNFSF10 Mouse None 170533 Rat IgG2A WB MAB1121 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
TRAIL/TNFSF10 Human � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
� Saliva
� Cell Lysate
7.87 pg/mL 15.6-1000 pg/mL DTRL00 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TRAIL/TNFSF10 Human 23.4 - 1500 pg/mL DY375 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TRAIL/TNFSF10 Human/Mouse RDP-63-025 25 Tests
TRAIL R1/TNFRSF10ARECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TRAIL R1/TNFRSF10A/Fc Chimera Human Lyophilized with a carrier protein None Sf 21 347-DR-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAIL R1/TNFRSF10A Human None Polyclonal Goat IgG B/N, IHC, WB AF347 100 µg
TRAIL R1/TNFRSF10A Human Biotin Polyclonal Goat IgG WB BAF347 50 µg
TRAIL R1/TNFRSF10A Human PE 69036 Mouse IgG1 FC FAB347P 100 Tests
TRAIL R1/TNFRSF10A Human None 69036 Mouse IgG1 FC MAB347 500 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TRAIL R1/TNFRSF10A Human RDP-64-025 25 Tests
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 53
TNF Superfamily
TRAIL R2/TNFRSF10BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TRAIL R2/TNFRSF10B/Fc Chimera
Human Lyophilized with a carrier protein None NS0 631-T2-100 100 µg
TRAIL R2/TNFRSF10B/Fc Chimera
Mouse Lyophilized without a carrier protein None Sf 21 721-DR-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAIL R2/TNFRSF10B Human None Polyclonal Goat IgG FA, WB AF631 100 µg
TRAIL R2/TNFRSF10B Human Biotin Polyclonal Goat IgG WB BAF631 50 µg
TRAIL R2/TNFRSF10B Human PE 71908 Mouse IgG2B FC FAB6311P 100 Tests
TRAIL R2/TNFRSF10B Human None 71903 Mouse IgG1 FA MAB631 500 µg
TRAIL R2/TNFRSF10B Human None 71908 Mouse IgG2B FC MAB6311 500 µg
TRAIL R2/TNFRSF10B Mouse None Polyclonal Goat IgG B/N, FC, WB AF721 100 µg
TRAIL R2/TNFRSF10B Mouse Biotin Polyclonal Goat IgG FC, WB BAF721 50 µg
TRAIL R2/TNFRSF10B Mouse None 118929 Rat IgG2A IHC, WB MAB1540 500 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TRAIL R2/TNFRSF10B Human RDP-68-025 25 Tests
TRAIL R3/TNFRSF10CRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TRAIL R3/TNFRSF10C/Fc Chimera
Human Lyophilized with a carrier protein None NS0 630-TR-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAIL R3/TNFRSF10C Human None Polyclonal Goat IgG B/N, IHC, WB AF630 100 µg
TRAIL R3/TNFRSF10C Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF630 50 µg
TRAIL R3/TNFRSF10C Human PE 90906 Mouse IgG1 FC FAB6302P 100 Tests
TRAIL R3/TNFRSF10C Human None 90903 Mouse IgG1 B/N MAB630 500 µg
TRAIL R3/TNFRSF10C Human None 90905 Mouse IgG1 ELISA(Cap), IHC, WB MAB6301 500 µg
TRAIL R3/TNFRSF10C Human None 90906 Mouse IgG1 FC MAB6302 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TRAIL R3/TNFRSF10C Human 62.5 - 4000 pg/mL DY630 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TRAIL R3/TNFRSF10C Human RDP-69-025 25 Tests
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037354
TRAIL R4/TNFRSF10DRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TRAIL R4/TNFRSF10D/Fc Chimera
Human Lyophilized without a carrier protein None NS0 633-TR-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAIL R4/TNFRSF10D Human None Polyclonal Goat IgG B/N, FC, IHC, WB AF633 100 µg
TRAIL R4/TNFRSF10D Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF633 50 µg
TRAIL R4/TNFRSF10D Human PE 104918 Mouse IgG1 FC FAB633P 100 Tests
TRAIL R4/TNFRSF10D Human None 104918 Mouse IgG1 B/N, ELISA(Cap), FC,WB
MAB633 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TRAIL R4/TNFRSF10D Human 93.8 - 6000 pg/mL DY633 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TRAIL R4/TNFRSF10D Human RDP-79-025 25 Tests
TRANCE/RANK L/TNFSF11RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TRANCE/TNFSF11Truncated
Human Lyophilized with a carrier protein None NS0 390-TN-010 10 µg
TRANCE/TNFSF11 Mouse Lyophilized with a carrier protein None NS0 462-TR-010 10 µg
TRANCE/TNFSF11 Mouse Lyophilized with a carrier protein None E. coli 462-TEC-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRANCE/TNFSF11 Human None Polyclonal Total Goat IgG AB626 1 mg
TRANCE/TNFSF11 Human None Polyclonal Goat IgG B/N, WB AF626 100 µg
TRANCE/TNFSF11 Human Biotin Polyclonal Goat IgG WB BAF626 50 µg
TRANCE/TNFSF11 Human None 70525 Mouse IgG2B B/N, IHC MAB626 500 µg
TRANCE/TNFSF11 Human None 70513 Mouse IgG1 WB MAB6261 500 µg
TRANCE/TNFSF11 Human None 175707 Mouse IgG1 B/N MAB6262 500 µg
TRANCE/TNFSF11 Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,WB
AF462 100 µg
TRANCE/TNFSF11 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF462 50 µg
TRANCE/TNFSF11 Mouse None 88227 Rat IgG2A WB MAB462 500 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
TRANCE/TNFSF11 Mouse � Serum
� Cell Culture Supernate
5 pg/mL 31.2-2000 pg/mL MTR00 1 Kit
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TRANCE/TNFSF11 Mouse 62.5 - 4000 pg/mL DY462 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 55
For antibody application key, see page 120.
TNF Superfamily
TRANCE/RANK L/TNFSF11 continuedPRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TRANCE/TNFSF11 Human/Mouse RDP-66-025 25 Tests
TROY/TNFRSF19RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TROY/TNFRSF19/Fc Chimera Human Lyophilized without a carrier protein None NS0 1548-TR-100 100 µg
TROY/TNFRSF19/Fc Chimera Mouse Lyophilized without a carrier protein None Sf 21 723-TR-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TROY/TNFRSF19 Human None Polyclonal Goat IgG WB AF1548 100 µg
TROY/TNFRSF19 Human Biotin Polyclonal Goat IgG WB BAF1548 50 µg
TROY/TNFRSF19 Mouse None Polyclonal Goat IgG ELISA(Cap), IHC, WB AF723 100 µg
TROY/TNFRSF19 Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF723 50 µg
TROY/TNFRSF19 Mouse None 323606 Rat IgG2A WB MAB723 100 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TROY/TNFRSF19 Mouse 62.5 - 4000 pg/mL DY723 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
TWEAK/TNFSF12RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TWEAK/TNFSF12 Human Lyophilized with a carrier protein None E. coli 1090-TW-025 25 µg
TWEAK/TNFSF12 Mouse Lyophilized with a carrier protein None E. coli 1237-TW-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TWEAK/TNFSF12 Human None Polyclonal Goat IgG B/N, WB AF1090 100 µg
TWEAK/TNFSF12 Human Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1090 50 µg
TWEAK/TNFSF12 Human None 173830 Mouse IgG1 ELISA(Cap) MAB1090 500 µg
TWEAK/TNFSF12 Mouse None Polyclonal Goat IgG B/N, WB AF1237 100 µg
TWEAK/TNFSF12 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1237 50 µg
TWEAK/TNFSF12 Mouse None 189803 Rat IgG2A ELISA(Cap) MAB1237 500 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037356
For antibody application key, see page 120.
TWEAK/TNFSF12 continuedDUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TWEAK/TNFSF12 Human 62.5 - 4000 pg/mL DY1090 15 Plates
TWEAK/TNFSF12 Mouse 15.6 - 1000 pg/mL DY1237 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
TWEAK/TNFSF12 Mouse/Rat RDP-120-025 25 Tests
TWEAK R/TNFRSF12RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
TWEAK R/TNFRSF12/Fc Chimera
Human Lyophilized without a carrier protein None NS0 1199-TW-050 50 µg
TWEAK R/TNFRSF12/Fc Chimera
Mouse Lyophilized without a carrier protein None Sf 21 1610-TW-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TWEAK R/TNFRSF12 Human None Polyclonal Goat IgG B/N, IHC, WB AF1199 100 µg
TWEAK R/TNFRSF12 Human Biotin Polyclonal Goat IgG WB BAF1199 50 µg
TWEAK R/TNFRSF12aa 28-79
Human None 314502 Rat IgG2A WB MAB1199 100 µg
TWEAK R/TNFRSF12 Mouse None Polyclonal Goat IgG ELISA(Cap), FC, IHC,WB
AF1610 100 µg
TWEAK R/TNFRSF12 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1610 50 µg
TWEAK R/TNFRSF12 Mouse None 314615 Rat IgG2B WB MAB1610 100 µg
TWEAK R/TNFRSF12 Mouse None 314636 Rat IgG2A FC, WB MAB16101 100 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
TWEAK R/TNFRSF12 Mouse 312 - 20000 pg/mL DY1610 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
XEDARRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
XEDAR/Fc Chimera Human Lyophilized without a carrier protein None NS0 1093-XD-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
XEDAR Human None Polyclonal Goat IgG B/N, WB AF1093 100 µg
XEDAR Human Biotin Polyclonal Goat IgG ELISA(Det), WB BAF1093 50 µg
XEDAR Human None 173111 Mouse IgG1 B/N, WB MAB1093 500 µg
XEDAR Human None 173103 Mouse IgG1 ELISA(Cap), WB MAB10931 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
XEDAR Human 15.625 - 1000 pg/mL DY1093 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy pack . Standard DuoSet Kits are also available in 45 plate Economy Pack .
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 57
TNF Superfamily
ss
Apoptosis Adaptor Proteins
Pro-Apoptotic Signaling Adaptors
CRADD/RAIDDANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CRADD Human/Mouse/Rat None Polyclonal Goat IgG WB AF4680 100 µg
CrkANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Crkaa 205-304
Human None Polyclonal Sheep IgG WB AF4316 100 µg
R&D Systems Tools for Cell Biology Research™
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037358
Apoptotic adaptor proteins play a critical role in regulating pro-
and anti-apoptotic signaling pathways following activation of the
death receptors. Adaptor proteins, such as FADD (Fas-associated
death domain) and TRADD (TNF receptor-associated death do-
main), are recruited to ligand-activated, oligomerized death
receptors to mediate apoptotic signaling pathways. Apoptotic ad-
aptors associate with the cytoplasmic portion of the death
receptors through a domain common to both known as the death
domain (DD). Following association with the ligand-bound recep-
tor, the adaptor proteins recruit Pro-caspase-8 or Pro-caspase-10
by way of their death effector domains (DED) to form the DISC
(death-inducing signaling complex). Formation of the DISC initi-
ates a caspase signaling cascade that ultimately induces apoptosis.
Other adaptor proteins mediate apoptotic signaling through dis-
tinct mechanisms. CRADD/RAIDD (caspase and RIP adaptor with a
death domain), and PIDD (p53-induced protein with a death do-
main) are two adaptor proteins that associate with Pro-caspase-2
to form the PIDDosome following DNA damage. Formation of this
complex leads to the cleavage and activation of Caspase-2. DAXX
(Fas death domain-associated-xx) and RIP1 (receptor interacting
protein 1) are two additional adaptor proteins containing a death
domain that are recruited to Fas or to TNF RI respectively, upon
ligand binding. These two proteins are involved in mediating
caspase-independent apoptotic signaling pathways. Like TNF RI
and Fas, TRAIL receptors (TRAIL R1/DR4 or TRAIL R2/DR5) and DR3
also contain intracellular death domains and can induce apoptosis
by way of adaptor-mediated Caspase-8 or Caspase-10 recruitment.
In addition to promoting apoptosis, ligand binding to TNF RI
(TNF�), or DR3 (TWEAK) can cause the intracellular adaptor pro-
teins to recruit TRAF-2 (TNF Receptor-associated factor 2). TRAF-2
signaling subsequently leads to the activation of NF�B which in-
duces the expression of anti-apoptotic genes such as FLIP and
Bcl-2.
Fo r m o r e i n f o r m a t i o n o n p r o d u c t s r e l a t e d t o A p o p t o s i s
Ad a p t o r Pr o t e i n s, p l e a s e v i s i t o u r w e b s i t e a t
www.RnDSystems.com/go/ApoptosisAdaptor
Detection of FADD in Human Cell Lysates. FADD was detected in lysates prepared
from human A431 epithelial carcinoma cells, Ramos and Raji Burkitt’s lymphoma-
derived cells, and MCF-7 breast cancer cells by Western blot analysis using anti-human
FADD polyclonal antibody (Catalog # AF2938).
Intracellular Detection of TRADD in Human Lymphocytes by Flow Cytometry.
Human lymphocytes were stained with APC-conjugated anti-human TRADD
monoclonal antibody (Catalog # IC2658A; filled histogram) or with APC-conjugated
mouse IgG2A isotype control antibody (Catalog # IC003A; open histogram).
DAXXANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
DAXXaa 497-626
Human None Polyclonal Goat IgG WB AF4548 100 µg
FADDANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
FADD Human None Polyclonal Goat IgG WB AF2938 100 µg
RIP1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RIP1 Human/Mouse/Rat None 334640 Mouse IgG1 WB MAB3585 100 µg
TRADDANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRADD Human None Polyclonal Goat IgG IHC, WB AF2658 100 µg
TRADD Human APC 313203 Mouse IgG2A FC IC2658A 100 Tests
TRADD Human Fluorescein 313203 Mouse IgG2A FC IC2658F 100 Tests
TRADD Human PE 313203 Mouse IgG2A FC IC2658P 100 Tests
TRADD Human None 313203 Mouse IgG2A FC, WB MAB2658 100 µg
Anti-Apoptotic Signaling Adaptors
14-3-3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
14-3-3 Human/Mouse/Rat/Xenopus
None Polyclonal Rabbit IgG WB PPS043 100 µL
Phospho-14-3-3 (S58) Human/Mouse/Rat/Xenopus
None Polyclonal Rabbit IgG WB PPS042 100 µL
14-3-3�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
14-3-3� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4724 100 µg
14-3-3�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
14-3-3� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4419 100 µg
14-3-3�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
14-3-3� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4420 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 59
For antibody application key, see page 120.
Apoptosis Adaptor Proteins
14-3-3�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
14-3-3� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4424 100 µg
14-3-3�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
14-3-3� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4425 100 µg
14-3-3�RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
14-3-3�HRP-conjugated
Human Solution with a carrier protein HRP E. coli HRP2669 250 µL
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
14-3-3� Human/Mouse None Polyclonal Goat IgG WB AF2669 100 µg
CD2APANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CD2APaa 423-580
Human/Mouse/Rat None Polyclonal Sheep IgG WB AF4474 100 µg
DFF45/ICADANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
DFF45/ICAD Human None Polyclonal Goat IgG WB AF4679 100 µg
FLIPRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
FLIPWestern Blot Standard
Human Lyophilized without a carrier protein None E. coli WBC001 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
FLIPaa 432-450
Human/Mouse None Polyclonal Rabbit IgG WB AF821 100 µg
SODD/BAG4ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SODD/BAG4aa 122-457
Human None Polyclonal Goat IgG WB AF4350 100 µg
SODD/BAG4 Mouse/Rat None Polyclonal Goat IgG WB AF4349 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037360
Other Apoptosis-related Signaling Molecules
MyD88ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MyD88 Human None Polyclonal Goat IgG FC, IHC, WB AF2928 100 µg
MyD88 Human None 316603 Rat IgG2A IHC MAB2928 100 µg
MyD88 Human None 316628 Rat IgG2B FC MAB29281 100 µg
MyD88 Mouse None 316902 Rat IgG2A IHC MAB3109 100 µg
MyD88 Mouse/Rat None Polyclonal Goat IgG FC, IHC, WB AF3109 100 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
MyD88 Human 312.5 - 20000 pg/mL DY2928 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
TANKANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TANKaa 157-448
Human/Mouse None Polyclonal Goat IgG WB AF4755 100 µg
TRAF-1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAF-1 Human None Polyclonal Goat IgG WB AF3276 100 µg
TRAF-2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAF-2 Human None Polyclonal Goat IgG WB AF3277 100 µg
TRAF-2 Human/Mouse/Rat None 324522 Mouse IgG2A WB MAB3277 100 µg
TRAF-3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAF-3Isoform 2
Human None Polyclonal Goat IgG WB AF3278 100 µg
TRAF-3Isoform 2
Human/Mouse/Rat None 433113 Rat IgG2A WB MAB3278 250 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 61
For antibody application key, see page 120.
Apoptosis Adaptor Proteins
TRAF-4ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAF-4 Human None Polyclonal Goat IgG WB AF3279 100 µg
TRAF-4 Human None 433709 Rat IgG2A WB MAB3279 250 µg
TRAF-6ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAF-6 Human None Polyclonal Goat IgG WB AF3284 100 µg
TRAM/TICAM2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TRAM/TICAM2 Human/Mouse/Rat None Polyclonal Goat IgG WB AF4348 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037362
Intrinsic Pathway of Caspase Activation
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 63
The intrinsic pathway of caspase activation can be initiated in re-
sponse to DNA damage, growth factor withdrawal, loss of contact
with the extracellular matrix, or glucocorticoids. These conditions
promote signaling pathways that lead to a loss in the integrity of
the mitochondrial membrane and caspase activation. The Bcl-2
family comprises a group of structurally related proteins that play a
fundamental role in the regulation of the intrinsic pathway by con-
trolling mitochondrial membrane permeability and the release of
the pro-apoptotic factor, Cytochrome c. Bcl-2 proteins are grouped
into three classes: those that inhibit apoptosis (Bcl-2, Bcl-xL, Bcl-w,
Mcl-1, Bcl-10, and Bcl-2 related protein A1); those that promote
apoptosis (BAK, Bax, Bcl-rambo, Bcl-xs, BOK/Mtd); and the
pro-apoptotic BH3-only proteins that bind and regulate the
anti-apoptotic Bcl-2 proteins (Bad, BID, Bik/Nbk, BIM, BLK, Bmf,
Hrk/DP5). Bag-1, BNIP3, BNIP3L are Bcl-2-interacting proteins that
are also involved in modulating apoptosis. The balance between
pro- and anti-apoptotic family members determines whether or
not a cell will undergo apoptosis. Bcl-2 and Bcl-xL
inhibit apoptosis
by binding to the pro-apoptotic Bax and BAK proteins. In healthy
cells, BAD is phosphorylated and sequestered in the cytoplasm by
the adapter protein, 14-3-3. When cytoplasmic levels of free Bad in-
crease, Bcl-2 and Bcl-xL
bind to Bad and release Bax and BAK. Bax
and BAK, or processed forms of these proteins, can then insert into
the mitochondrial membrane and cause the release of
Cytochrome c. Cleavage of BID as a result of Caspase-8, or
Caspase-10 activation following death receptor ligation also pro-
motes Bax/BAK-mediated release of Cytochrome c.
For more information on products related to the Bcl-2 Family, please
visit our website at www.RnDSystems.com/go/Bcl2
Alignment of the BH Domains in the Bcl-2 Family. (A) Position of the Bcl-2 homology regions (BH1-4) and �-helices in Bcl-2 and Bcl-xL. The regions forming the hydrophobic
pocket, the BH3-ligand, and pore are indicated. (B) Alignment of the BH regions in the Bcl-2 family. TM indicates a membrane targeting sequence.
Bcl-2 Family
R&D Systems Tools for Cell Biology Research™
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037364
Bcl-xL Inhibition of tBID-induced Cytochrome c Release. Isolated mouse liver mito-
chondria were incubated with Caspase-8-cleaved recombinant human BID (Catalog #
882-B8; purple line) or with cleaved recombinant mouse BID (Catalog # 883-M8; blue
line) and increasing concentrations of Bcl-xL (Catalog # 878-BC). The amount of
Cytochrome c released was measured using the rat/mouse Cytochrome c ELISA Kit
(Catalog # MCTC0). The blank sample (filled diamond) indicates spontaneously re-
leased Cytochrome c; the total sample (X) indicates the total amount of Cytochrome c
in the mitochondria at the start of the experiment. Results are the averages of triplicate
measurements +/- SEM.
Quantification of Bcl-2 in Cell Lysates. The levels of Bcl-2 were assessed in lysates
prepared from human KG1 myelogenous leukemia cells and human MCF-7 breast can-
cer cells using the human Bcl-2 DuoSet® IC ELISA Development System (Catalog #
DYC827B; histogram). The same lysates were also analyzed by Western blot using
anti-human Bcl-2 monoclonal antibody (Catalog # MAB827; inset).
Quantification of Bcl-xL in Cell Lysates. The levels of Bcl-xL were assessed in lysates
prepared from mouse NIH/3T3 fibroblast cells and human A431 carcinoma cells using
the human/mouse Bcl-xL DuoSet® IC ELISA Development System (Catalog # DYC894;
histogram). The same lysates were also analyzed by Western blot using anti-hu-
man/mouse Bcl-xL monoclonal antibody (Catalog # MAB827; inset).
Neutralization of tBID-induced Cytochrome c Release. Caspase-8-cleaved recombi-
nant human BID (Catalog # 882-B8; hBID), or cleaved recombinant mouse BID (Catalog
# 883-M8; mBID) was incubated alone or with anti-human/mouse BID polyclonal anti-
body (Catalog # AF860) for 30 (gray bars) or 60 minutes (yellow bars) prior to the
addition of isolated mouse liver mitochondria. The amount of Cytochrome c released
was measured using the rat/mouse Cytochrome c ELISA Kit (Catalog # MCTC0). The
blank sample indicates spontaneously released Cytochrome c; the total sample indi-
cates the total amount of Cytochrome c in the mitochondria at the start of the
experiment. Results are the averages of triplicate measurements.
For antibody application key, see page 120.
BadANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Badaa 1- 21
Human/Mouse None Polyclonal Rabbit IgG WB AF819 100 µg
BadN-Terminus
Mouse None 64103 Rat IgG2A WB MAB850 100 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Bad Human/Mouse/Rat RDP-41-025 25 Tests
Bag-1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bag-1 Human None GP3.10G3E2 Mouse IgG1 IP, WB MAB852 100 µg
Bag-1 Mouse None Polyclonal Goat IgG IP, WB AF815 100 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Bag-1 Human/Mouse RDP-81-025 25 Tests
BAKRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
BAKBH3 Domainaa 72-87
Human Lyophilized without a carrier protein None Synthetic 881-BA-100 100 µg
BAKL78A BH3 Domainaa 72-87
Human Lyophilized without a carrier protein None Synthetic 879-BK-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BAKaa 53-72
Human None Polyclonal Rabbit IgG WB AF816 100 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
BAK Human/Mouse RDP-42-025 25 Tests
BaxANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Baxaa 3-16
Human None YTH-2D2 Mouse IgG1 IP, WB 2282-MC-100 100 µg
BaxMinus C-Terminus
Human None Polyclonal Total Goat IgG IHC, WB AB820 400 µg
BaxMinus C-Terminus
Human None 127606 Mouse IgG2B WB MAB846 100 µg
Baxaa 12-33 (human)
Human/Mouse None Polyclonal Rabbit IgG IHC, WB AF820 30 µg
BaxMinus C-Terminus
Human/Mouse None 127602 Mouse IgG2A WB MAB883 100 µg
Baxaa 12-24 (human)
Human/Mouse/Rat None YTH-6A7 Mouse IgG1 IP, WB 2281-MC-100 100 µg
Baxaa 3-16
Mouse None YTH-5B7 Mouse IgG1 IP, WB 2280-MC-100 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 65
Bcl-2 Family
Bax continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
BaxTotal Protein
Human 312 - 20000 pg/mL DYC820-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Bax Human/Mouse/Rat RDP-43-025 25 Tests
Bcl-10ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bcl-10 Human None Polyclonal Goat IgG WB, IHC AF2889 100 µg
Bcl-10 Human/Mouse/Rat None 384202 Mouse IgG1 WB, IHC MAB2889 100 µg
Bcl-2RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Bcl-2Minus C-Terminus
Human Solution without a carrier protein None E. coli 827-BC-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bcl-2aa 41-54
Human None YTH-8C8 Mouse IgG1 IP, WB 2291-MC-100 100 µg
Bcl-2Minus C-Terminus
Human None 118701 Mouse IgG1 WB MAB827 100 µg
Bcl-2Minus C-Terminus
Human None 118712 Mouse IgG1 B/N MAB8271 100 µg
Bcl-2aa 1-208 (mouse)
Human/Mouse None Polyclonal Goat IgG IHC, IP, WB AF810 100 µg
Bcl-2aa 4-22
Human/Mouse None Polyclonal Rabbit IgG WB AF827 150 µg
Bcl-2Minus C-Terminus
Mouse None 121529 Rat IgG2B WB MAB810 100 µg
Bcl-2aa 61-76 (mouse)
Mouse/Rat None YTH-10C4 Mouse IgG1 IP, WB 2290-MC-100 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Bcl-2Total Protein
Human 62.5 - 4000 pg/mL DYC827B-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Bcl-2 Human/Mouse/Rat RDP-44-025 25 Tests
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037366
For antibody application key, see page 120.
Bcl-2 related protein A1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Bcl-2 related protein A1Minus C-terminusaa 1-152
Human Solution without a carrier protein None E. coli 1160-A1-050 50 µg
Bcl-2 related protein A1Western Blot Standard
Mouse Solution without a carrier protein None N/A WBC011 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bcl-2 related protein A1aa 1-152
Mouse None 78616 Rat IgG2A WB MAB851 100 µg
Bcl-wRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Bcl-wMinus C-Terminus
Human Solution without a carrier protein None E. coli 824-BW-050 50 µg
Bcl-wWestern Blot Standard
Human Solution without a carrier protein None E. coli WBC005 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bcl-waa 1-172
Human None 90316 Mouse IgG1 WB MAB824 100 µg
Bcl-waa 1-172
Human None 90336 Mouse IgG1 B/N MAB8241 250 µg
Bcl-waa 1-172
Human/Mouse None Polyclonal Goat IgG IHC AF8241 100 µg
Bcl-waa 32-51
Human/Mouse None Polyclonal Rabbit IgG WB AF824 120 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Bcl-w Human/Mouse RDP-125-025 25 Tests
Bcl-xANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bcl-xaa 49-68
Human None Polyclonal Sheep IgG IP, WB AF800 50 µg
Bcl-xL
RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Bcl-xL
Minus C-TerminusHuman Solution without a carrier protein None E. coli 894-BX-050 50 µg
Bcl-xL
Minus C-TerminusMouse Solution without a carrier protein None E. coli 878-BC-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bcl-xL
aa 3-14 (human)Human/Mouse/Rat None YTH2H12 Mouse IgG2A IP, WB 2300-MC-100 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 67
Bcl-2 Family
Bcl-xL continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Bcl-xL
Total ProteinHuman 625 - 20000 pg/mL DYC894-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
BIDRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
BID Human Solution without a carrier protein None E. coli 846-BD-050 50 µg
BIDCaspase-8-cleaved
Human Solution without a carrier protein None E. coli 882-B8-050 50 µg
BID Mouse Solution without a carrier protein None E. coli 860-MB-050 50 µg
BIDCaspase-8-cleaved
Mouse Solution without a carrier protein None E. coli 883-M8-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BIDaa 34-52
Human None Polyclonal Rabbit IgG WB AF846 100 µg
BID Human/Mouse None Polyclonal Goat IgG IP, WB AF860 100 µg
BID Mouse Biotin Polyclonal Goat IgG WB BAF860 50 µg
BID Mouse None 91508 Rat IgG1 WB MAB860 100 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
BID Human RDP-110-025 25 Tests
BID Mouse RDP-111-025 25 Tests
Bik-like Killer ProteinRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Bik-like Killer ProteinWestern Blot Standard
Mouse Solution without a carrier protein None E. coli WBC007 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Bik-like Killer Proteinaa 25-42
Mouse None Polyclonal Rabbit IgG WB AF849 100 µg
BIMRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
BIMLong Form
Human Solution without a carrier protein None E. coli 1325-BL-050 50 µg
BNIP3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BNIP3 Human None Polyclonal Goat IgG WB AF4147 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037368
BNIP3LANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BNIP3Laa 2-184
Human/Mouse/Rat None Polyclonal Goat IgG WB AF4030 100 µg
BOKRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
BOKWestern Blot Standard
Rat Lyophilized without a carrier protein None E. coli WBC004 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
BOKaa 41-61
Rat None Polyclonal Rabbit IgG WB AF825 80 µg
HrkRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
HrkWestern Blot Standard
Human Solution without a carrier protein None E. coli WBC013 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Hrkaa 47-66
Human None Polyclonal Rabbit IgG WB AF851 100 µg
Mcl-1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Mcl-1Western Blot Standard
Human Solution without a carrier protein None E. coli WBC003 1 Vial
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Mcl-1aa 175-194
Human None Polyclonal Rabbit IgG WB AF828 150 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
Mcl-1 Human/Mouse RDP-82-025 25 Tests
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 69
For antibody application key, see page 120.
Bcl-2 Family
Mitochondrial Proteins
R&D Systems Tools for Cell Biology Research™
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037370
An alteration in the permeability of the mitochondrial membrane is
a hallmark of the intrinsic pathway of caspase activation. This
change in permeability leads to the cytosolic release of multiple
proteins, including Cytochrome c, Smac/Diablo, HTRA2/Omi,
Apoptosis Inducing Factor (AIF), and Endonuclease G from the
intermembrane space of the mitochondria. The release of these
proteins, which are normally sequestered, has severe conse-
quences for the cell. For example, cytoplasmic Cytochrome c and
dATP initiate the assembly of the apoptosome with APAF-1 and
Pro-caspase-9. The assembly of this complex promotes the activa-
tion of Caspase-9 which subsequently leads to the activation of
Caspase-3, -6, and -7 to induce apoptosis. In addition, Smac/Diablo
and HTRA2/Omi interact with the IAP proteins disrupting their abil-
ities to inhibit caspase activity, while AIF and Endonuclease G
translocate to the nucleus upon mitochondrial release and pro-
mote chromatin condensation and DNA fragmentation.
For more information on Mitochondrial Protein products, please visit
our website at www.RnDSystems.com/go/Mitochondria
Pro-apoptotic Proteins Released from the Mitochondria. The intrinsic pathway of caspase activation or Caspase-8-dependent cleavage of BID causes a change in the perme-
ability of the mitochondrial membrane leading to the release of multiple pro-apoptotic proteins, including Cytochrome c, SMAC/Diablo, Apoptosis Inducing Factor (AIF) and
Endonuclease G (Endo G).
For antibody application key, see page 120.
AIFANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
AIF Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1457 100 µg
Cytochrome cRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Cytochrome cNatural Source
Human Lyophilized without a carrier protein None Human Heart 709-CCH-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Cytochrome c Human/Equine None 2B5F8 Mouse IgG2A IHC, IP MAB898 100 µg
Cytochrome c Human/Mouse/Rat None 4CytC-21 Mouse IgG1 WB 6370-MC-100 100 µg
Cytochrome c Human/Mouse/Rat None 7H8.2C12 Mouse IgG2B WB MAB897 100 µg
Cytochrome c Human/Mouse/Rat None 2.7D5 Mouse IgG2A IP MAB899 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 71
Kinetics of tBID-induced Cytochrome c Release.
Isolated mouse liver mitochondria were untreated (orange
line), or incubated with either 52 nM (light blue line), or
5.2 nM (green line) Caspase-8-cleaved recombinant hu-
man BID (Catalog # 882-B8) for the indicated times. The
amount of Cytochrome c released was determined using
the rat/mouse Cytochrome c ELISA (Catalog # MCTC0).
Inhibition of XIAP Activity by SMAC/Diablo.
Cytochrome c and dATP-activated human Jurkat T cell
extracts were treated with recombinant human XIAP
(Catalog # 822-XF; 500 nM) and increasing concentra-
tions of recombinant human SMAC/Diablo (Catalog
#789-SM) prior to the addition of the caspase peptide
substrate, DEVD-afc. DEVD-afc cleavage was monitored
in a fluorescence plate reader.
SMAC/Diablo in Jurkat and CHP-100 Cells. Cell lysates
from human Jurkat T cells and CHP-100 neuroblastoma
cells were assayed for levels of total SMAC/Diablo using
the human SMAC/Diablo DuoSet® IC ELISA Development
System (Catalog # DYC789; histogram). The lysates were
also analyzed by Western blot using an anti-human
SMAC/Diablo polyclonal antibody (Catalog # AF789; inset).
Mitochondrial Proteins
Cytochrome c continuedELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Cytochrome c Human � Cell Lysate
� Subcellular Fractions(optimal protocol to bedetermined by end user)
0.31 ng/mL 0.625-20 ng/mL DCTC0 1 Kit
Cytochrome c Mouse/Rat � Cell Lysate
� Subcellular Fractions(optimal protocol to bedetermined by end user)
0.5 ng/mL 0.78-25 ng/mL MCTC0 1 Kit
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Cytochrome cTotal Protein-Short Format
Mouse/Rat N/A DYC897-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
Holocytochrome cANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Holocytochrome c Human/Mouse/Rat None 2CtyC-199 Mouse IgG1 IP, WB 6380-MC-100 100 µg
HTRA2/OmiRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
HTRA2/Omi Human Solution without a carrier protein None E. coli 1458-HT-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HTRA2/Omi Human None 229926 Mouse IgG2B IHC, WB MAB1458 100 µg
HTRA2/Omi Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1458 100 µg
SMAC/DiabloRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
SMAC/Diablo Human Solution without a carrier protein None E. coli 789-SM-100 100 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SMAC/Diablo Human None Polyclonal Rabbit IgG WB AF789 100 µg
SMAC/Diablo Human None Polyclonal Goat IgG IHC, WB AF7891 100 µg
SMAC/Diablo Human Biotin Polyclonal Goat IgG WB BAF789 50 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
SMAC/DiabloTotal Protein
Human 62.5 - 4000 pg/mL DYC789-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
UCP2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
UCP2 Human/Mouse None Polyclonal Goat IgG WB AF4739 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037372
Oxidative Stress
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 73
Oxidative stress is a condition characterized by elevated levels of
intracellular reactive oxygen species (ROS). ROS either are, or break
down to form, free radicals. ROS include superoxide anion (O2-),
hydrogen peroxide (H2O
2), and hydroxyl radicals (OH-) that are
capable of reacting with, and damaging DNA, proteins, and lipids.
Under normal conditions, ROS are cleared from the cell by the ac-
tions of superoxide dismutase, catalase, glutathione peroxidase,
thioredoxin, and other molecules. Low levels of intracellular ROS
have also been identified as second messengers in signaling path-
ways and implicated in transcriptional regulation to promote cell
proliferation, but higher doses of ROS result in growth arrest and
cell death. Bcl-2 inhibits free-radical induced apoptosis. Whether
the result is cell survival or apoptosis, oxidative stress activates nu-
merous intracellular signaling pathways such as Akt, NF�B, p53,
Jak/Stat, and MAPK to affect changes in gene expression.
For more information on products related to Oxidative Stress, please
visit our website at www.RnDSystems.com/go/OxStress
Oxidative Stress and the Mitochondria. Mitochondrial-derived ROS, such as H2O2, generated by defects in the electron transport chain (ETC Complex I) can react with iron or
copper in the cytoplasm to generate free radicals. ROS can also be generated by nitric oxide synthase (NOS) which is activated by faulty cytoplasmic calcium homeostasis and
mitochondrial calcium accumulation. In addition, mitochondrial calcium accumulation and a decrease in mitochondrial ROS scavenging capability leads to the opening of the
mitochondrial transition pore (MTP) and the release of Cytochrome c. Free radicals and cytoplasmic Cytochrome c promote apoptosis.
Detection of SOD1 in Human Cell Lysates. SOD1 was detected in cell lysates pre-
pared from human Jurkat T cells and Ramos Burkitt’s lymphoma cells by Western blot
analysis using anti-human SOD1 monoclonal antibody (Catalog # MAB3418; left). This
antibody failed to recognize recombinant human SOD2 or SOD3, demonstrating
specificity for SOD1 (right).
eNOS Expression in Human Placenta. eNOS was detected in paraffin-embedded
tissue sections of human placenta using anti-human eNOS polyclonal antibody
(Catalog # AF950). Tissues were stained using anti-goat HRP-AEC Cell and Tissue Stain-
ing Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).
Oxidative Stress
Carboxymethyl LysineANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Carboxymethyl Lysine Multi-species None 318003 Mouse IgG2B WB MAB3247 100 µg
CatalaseANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Catalase Human/Mouse/Rat None Polyclonal Goat IgG WB AF3398 100 µg
Glutaredoxin 1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Glutaredoxin 1 Human/Mouse None Polyclonal Goat IgG WB AF3399 100 µg
Glutaredoxin 1 Mouse None Polyclonal Goat IgG WB AF3119 100 µg
Glutathione Peroxidase 1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Glutathione Peroxidase 1 Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF3798 100 µg
Glutathione Peroxidase 3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Glutathione Peroxidase 3aa 74-226
Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF4199 100 µg
Glyoxalase IRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Glyoxalase I Human Lyophilized without a carrier protein None E. coli 4959-GL-02M 2500 µg
HO-1/HMOX1/HSP32ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HO-1/HMOX1/HSP32aa 1-261 (human)
Human/Mouse None Polyclonal Goat IgG WB AF3776 100 µg
HO-1/HMOX1/HSP32aa 1-261 (human)
Human/Mouse None 412811 Rat IgG2B WB MAB3776 100 µg
HO-1/HMOX1/HSP32 Mouse/Rat None Polyclonal Goat IgG WB AF3169 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
HO-1/HMOX1/HSP32Total Protein
Human N/A DYC3776-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
HO-2/HMOX2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HO-2/HMOX2 Human/Mouse None 322913 Mouse IgG2A WB MAB3170 100 µg
HO-2/HMOX2 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3170 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037374
4-HydroxynonenalANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
4-Hydroxynonenal Multi-species None 198960 Mouse IgG2B IHC, WB MAB3249 100 µg
MyeloperoxidaseRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Myeloperoxidase Human Lyophilized without a carrier protein None NS0 3174-MP-250 250 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Myeloperoxidase Human None Polyclonal Goat IgG IHC, WB AF3174 100 µg
Myeloperoxidase Human Biotin Polyclonal Goat IgG WB BAF3174 50 µg
Myeloperoxidase Human/Mouse None Polyclonal Goat IgG IHC, WB AF3667 100 µg
Myeloperoxidase Human/Mouse None 392105 Mouse IgG2B IHC, WB MAB3174 100 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Myeloperoxidase Human � Serum
� EDTA and Heparin Plasma
� Cell Culture Supernate
� Urine
� Saliva
� Cell Lysate
� Breast Milk
0.618 ng/mL 1.56-100 ng/mL DMYE00 1 Kit
Nitric OxideELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Nitric Oxide Multi-species � Serum
� Citrate, EDTA, andHeparin Plasma
� Cell Culture Supernate
� Urine
0.78 nmol/mL 3.12-200 nmol/mL KGE001 1 Kit
NitrotyrosineANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Nitrotyrosine Multi-species None 306507 Mouse IgG3 WB MAB3248 100 µg
eNOSANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
eNOSaa 1030-1203
Human None Polyclonal Goat IgG IHC, IP, WB AF950 100 µg
eNOSaa 1030-1203
Human Biotin Polyclonal Goat IgG IHC, WB BAF950 50 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
eNOS Human � Cell Lysate 25 pg/mL 62.5-4000 pg/mL DEN00 1 Kit
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 75
For antibody application key, see page 120.
Oxidative Stress
eNOS continuedPRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
eNOS Human/Mouse RDP-94-025 25 Tests
iNOSANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
iNOSaa 781-798
Human None 2D2-B2 Mouse IgG1 IHC, WB MAB9502 100 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
iNOS Human � Cell Lysate 0.46 U/mL 1.25-80 U/mL DNS00 1 Kit
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
iNOS Human/Mouse RDP-101-025 25 Tests
nNOSANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
nNOS Human None Polyclonal Goat IgG IHC, WB AF2416 100 µg
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
nNOS Human/Mouse RDP-93-025 25 Tests
Park7/DJ-1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Park7/DJ-1 Human None Polyclonal Goat IgG IHC, WB AF3995 100 µg
Park7/DJ-1 Human Biotin Polyclonal Goat IgG WB BAF3995 50 µg
Park7/DJ-1 Human/Mouse None Polyclonal Goat IgG WB AF3668 100 µg
Peroxiredoxin 1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Peroxiredoxin 1 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3488 100 µg
Peroxiredoxin 2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Peroxiredoxin 2 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3489 100 µg
Peroxiredoxin 5ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Peroxiredoxin 5 Human/Mouse None Polyclonal Goat IgG WB AF3774 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037376
Peroxiredoxin 6ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Peroxiredoxin 6 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3490 100 µg
PON2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PON2aa 30-354
Human None Polyclonal Goat IgG WB AF4344 100 µg
PON3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PON3aa 30-354
Human/Mouse/Rat None Polyclonal Goat IgG WB AF4345 100 µg
SOD1/Cu-Zn SODANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SOD1/Cu-Zn SOD Human None 348808 Mouse IgG2A IHC, WB MAB3418 100 µg
SOD1/Cu-Zn SOD Human/Mouse None Polyclonal Goat IgG WB AF3418 50 µg
SOD1/Cu-Zn SOD Mouse/Rat None Polyclonal Goat IgG WB AF3787 100 µg
SOD2/Mn-SODANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SOD2/Mn-SODaa 25-222
Human/Mouse/Rat None 349810 Mouse IgG1 IHC, WB MAB3419 100 µg
SOD3/EC-SODANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SOD3/EC-SODaa 25-251
Mouse None Polyclonal Goat IgG WB AF4817 100 µg
Superoxide DismutaseELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Superoxide Dismutase Multi-species � Cell Extracts N/A N/A 7500-100-K 100 Tests
Thioredoxin-1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Thioredoxin-1 Human Lyophilized without a carrier protein None E. coli 1970-TX-500 500 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Thioredoxin-1 Human None Polyclonal Goat IgG IHC, WB AF1970 100 µg
Thioredoxin-1 Human Biotin Polyclonal Goat IgG WB BAF1970 50 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 77
For antibody application key, see page 120.
Oxidative Stress
Thioredoxin-2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Thioredoxin-2 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3254 100 µg
Thioredoxin-80RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Thioredoxin-80 Human Lyophilized without a carrier protein None E. coli 2329-TX-100 100 µg
Thioredoxin-like 5/TRP14ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Thioredoxin-like 5/TRP14 Human None Polyclonal Goat IgG WB AF3504 100 µg
Thioredoxin-like 5/TRP14 Mouse/Rat None Polyclonal Goat IgG WB AF3769 100 µg
TXNDC5ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
TXNDC5aa 110-432
Human None Polyclonal Goat IgG WB AF4840 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037378
Heat Shock Proteins (HSPs)
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 79
Heat shock proteins (HSPs) are a highly conserved family of stress
response proteins that are induced upon exposure to environmen-
tal stresses such as heat, cold, or oxidative stress. Most HSPs act as
molecular chaperones to facilitate the folding of cellular proteins,
prevent protein aggregation, and target improperly folded pro-
teins to specific degradative pathways. In addition, a subset of HSPs
regulate apoptotic signaling pathways. While some HSPs are
anti-apoptotic (HSP27, HSP70, HSP90), others promote apoptosis
(HSP10, HSP60). HSP27, HSP70, and HSP90 all inhibit the forma-
tion of the apoptosome by binding to either Cytochrome c
(HSP27), or APAF-1 (HSP70, HSP90), subsequently preventing the
maturation and activation of Caspase-9. HSP70 can also inhibit
apoptosis by blocking the release of Apoptosis Inducing Factor
(AIF) from the mitochondria. HSP60, with or without HSP10, pro-
motes the proteolytic processing of Pro-caspase-3 to induce
apoptosis.
For more information on HSP-related products, please visit our website
at www.RnDSystems.com/go/HSPs
Regulation of Apoptotic Signaling by the Heat Shock Proteins. Some heat shock proteins inhibit apoptosis by binding to Cytochrome c or APAF-1 to prevent the formation
of the apoptosome. Other heat shock proteins promote apoptosis by stimulating the activation of Caspase-3.
HSP27 Inhibits Caspase Activity in Activated Cell Ex-
tracts. Human Jurkat T cell extracts were incubated with
Cytochrome c, dATP, and increasing concentrations of re-
combinant human HSP27 (Catalog # 1580-HS) prior to
the addition of the DEVD-afc caspase peptide substrate.
DEVD-afc cleavage was monitored in a fluorescence
plate reader.
Detection of HSP70 in Human Jurkat and Mouse
NIH/3T3 Cells by Western Blot. Human Jurkat T cells
and mouse NIH/3T3 fibroblast cells were either un-
treated (-), or heat shocked (+) for 30 minutes at 42° C
and then allowed to recover for 3 hours. HSP70 in lysates
from treated and non-treated cells was detected by
Western blot analysis using anti-human/mouse/rat
HSP70 monoclonal antibody (Catalog # MAB1663).
Quantification of Phosphor ylated HSP27 in
UV-treated mouse C2C12 Cells by ELISA. Mouse
C2C12 myoblast cells were left untreated or were
treated with UV (200 J/m2). Cell lysates were assessed for
HSP27 phosphorylation on serines 78 and 82 using the
human/mouse/rat Phospho-HSP27 (S78/82) DuoSet® IC
ELISA Development System (Catalog # DYC2314; histo-
gram). The same lysates were also analyzed by Western
blot ( inset ) using anti -human/mouse/rat
Phospho-HSP27 (S78/S82) polyclonal antibody (Catalog
# AF2314) and anti-human/mouse/rat HSP27 polyclonal
antibody (Catalog # AF15801).
Heat Shock Proteins
Clusterin/ApolipoproteinANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Clusterin Human None Polyclonal Goat IgG IHC, IP, WB AF2937 100 µg
Clusterin Human None 350227 Mouse IgG2B IP, WB MAB2937 100 µg
Clusterin Human None 350217 Mouse IgG2B IHC MAB29371 100 µg
Clusterin Mouse None Polyclonal Goat IgG IHC, IP, WB AF2747 100 µg
Clusterin Mouse Biotin Polyclonal Goat IgG WB BAF2747 50 µg
Clusterin� Chain
Mouse None 327020 Rat IgG2A IP, WB MAB2747 100 µg
�A Crystallin/CRYAAANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
�A Crystallin/CRYAA Human/Mouse None Polyclonal Goat IgG WB AF4848 100 µg
�B Crystallin/CRYABANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
�B Crystallin/CRYAB Human/Mouse/Rat None Polyclonal Goat IgG WB AF4849 100 µg
GRP75/HSPA9BANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GRP75/HSPA9Baa 542-679
Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF3584 100 µg
GRP75/HSPA9Baa 645-649
Human/Mouse/Rat None 419612 Mouse IgG3 WB MAB3584 100 µg
GRP78/HSPA5ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GRP78/HSPA5 Human/Mouse/Rat None Polyclonal Goat IgG WB AF4846 100 µg
HO-1/HMOX1/HSP32ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HO-1/HMOX1/HSP32aa 1-261 (human)
Human/Mouse None Polyclonal Goat IgG WB AF3776 100 µg
HO-1/HMOX1/HSP32aa 1-261 (human)
Human/Mouse None 412811 Rat IgG2B WB MAB3776 100 µg
HO-1/HMOX1/HSP32 Mouse/Rat None Polyclonal Goat IgG WB AF3169 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
HO-1/HMOX1/HSP32Total Protein
Human N/A DYC3776-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037380
HSP10/EPFANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSP10/EPF Human None 330629 Mouse IgG2B WB MAB3298 100 µg
HSP10/EPFaa 2-102
Human/Mouse None Polyclonal Goat IgG WB AF3298 100 µg
HSP10/EPF Mouse Biotin Polyclonal Goat IgG WB BAF2584 50 µg
HSP10/EPFaa 2-102
Mouse None Polyclonal Goat IgG WB AF2584 100 µg
HSP20/HSPB6ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSP20/HSPB6 Rat None Polyclonal Goat IgG WB AF4200 100 µg
HSP27RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
HSP27 Human Solution without a carrier protein None E. coli 1580-HS-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSP27 Human/Mouse None Polyclonal Rabbit IgG WB AF1580 50 µg
HSP27 Human/Mouse/Rat None Polyclonal Goat IgG WB AF15801 50 µg
Phospho-HSP27 (S78/S82) Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF2314 50 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
HSP27Total Protein
Human 31.2 - 2000 pg/mL DYC1580-2 2 Plates
Phospho-HSP27 (S78/S82) Human/Mouse/Rat 62.5 - 4000 pg/mL DYC2314-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
HSP40/DNAJB1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSP40/DNAJB1 Human/Mouse None Polyclonal Sheep IgG WB AF4145 100 µg
HSP40/DNAJB1 Human/Mouse/Rat None 419401 Mouse IgG2B WB MAB4145 100 µg
HSP60ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSP60 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1800 50 µg
HSP60 Human/Mouse/Rat None 264233 Mouse IgG2B IHC, WB MAB1800 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
HSP60Total Protein
Human 625 - 40000 pg/mL DYC1800-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 81
For antibody application key, see page 120.
Heat Shock Proteins
HSP70ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSP70 Human/Mouse None 242707 Mouse IgG2A IHC, WB MAB1663 100 µg
HSP70 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1663 50 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
HSP70Total ProteinSurveyor IC ELISA
Human/Mouse/Rat � Cell Lysate N/A 156-10000 pg/mL SUV1663 1 Kit
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
HSP70Total Protein
Human/Mouse/Rat 156 - 10000 pg/mL DYC1663-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
CELL-BASED ASSAYS
ANALYTE SPECIES CATALOG # SIZE
HSP70Cell-Based ELISA
Human/Mouse KCB1663 1 Kit
HSP90ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSP90 Human None Polyclonal Goat IgG WB AF3286 100 µg
HSP90 Human/Mouse/Rat None Polyclonal Goat IgG WB AF3775 100 µg
HSP90 Human/Mouse/Rat None 341320 Mouse IgG2B WB MAB3286 100 µg
HSP90Isoform HSP90�
Human/Mouse/Rat None 341346 Mouse IgG2A WB MAB32861 100 µg
HSPA8/HSC71ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSPA8/HSC71aa 534-615
Human/Mouse/Rat None Polyclonal Goat IgG WB AF4148 100 µg
HSPH1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
HSPH1aa 595-733
Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF4029 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037382
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 83
Intracellular signaling pathways regulate the decision of whether a
cell lives or dies. While death ligands such as Fas and TNF-� bind to
their receptors to promote caspase activation and cell death,
anti-apoptotic ligands, such as growth factors, bind to cell surface
receptors and activate intracellular kinases that promote cell sur-
vival. These kinases include PI 3-K, Akt/PKB, PKA, PKC, MEKs,
ERK1/2, p90RSK, and p38 MAP kinases. Intracellular kinases ulti-
mately activate or inhibit other cellular proteins involved in
apoptosis, or transcription factors that inhibit the expression of
pro-apoptotic proteins, or promote the expression of cell survival
proteins. For example, activation of PI 3-K leads to the
phosphorylation and activation of Akt/PKB resulting in the Akt-de-
pendent phosphorylation and inhibition of pro-apoptotic
molecules such as Bad, GSK-3, Caspase-9, and the Forkhead family
of transcription factors (FKHR). Similarly, other intracellular kinases
activate transcription factors such as CREB and STAT family mem-
bers which induce the expression of Bcl-2 and Bcl-xL, two Bcl-2
family members that protect the cell from apoptosis.
For more information on Intracellular Kinase products, please visit our
website at www.RnDSystems.com/go/ApoptosisICKinases
Intracellular Kinases Activated by Survival Signals Suppress Apoptosis. The binding of growth factors or cytokines to their receptors activates intracellular kinases that
phosphorylate and inhibit pro-apoptotic members of the Bcl-2 family, GSK-3, Caspase-9, and the Forkhead family of transcription factors. Intracellular kinases can also activate
transcription factors which induce the expression of Bcl-2 and Bcl-xL to promote cell survival (not shown).
Intracellular Detection of Phosphorylated Akt (S473)
in Jurkat Cells by Flow Cytometry. Human Jurkat T
cells, untreated (green histogram) or treated with the PI
3-kinase inhibitors LY294002 and wortmannin (purple
histogram), were stained using carboxyfluorescein
(CFS)-conjugated anti-human/mouse/rat Phospho-Akt
(S473) polyclonal antibody (Catalog # IC887F). Treated
cells were also stained with CFS-conjugated rabbit IgG
isotype control antibody (Catalog # IC105F; open histo-
gram).
Phosphorylated ERK1/ERK2 in Rat Brain Cortex.
Phosphorylation of ERK1/ERK2 was detected in cryostat
tissue sections of rat brain cortex using anti-hu-
man/mouse/rat Phospho-ERK1 (T202/Y204)/ERK2
(T185/Y187) polyclonal antibody (Catalog # AF1018). Tis-
sues were stained using anti-rabbit HRP-DAB Cell and
Tissue Staining Kit (Catalog # CTS005; brown) and
counterstained with hematoxylin (blue).
Phosphorylated MEK1/2 in PMA-treated Human
HeLa Cells. Human HeLa cells were uninduced or
treated with PMA (200 nM) for 20 minutes. Cell lysates
were assessed for MEK1 (S218/S222) and MEK2
(S222/S226) phosphorylation using the human
Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) DuoSet®
IC ELISA Development System (Catalog # DYC2506; his-
togram). The same lysates were also immunoblotted
(inset) using anti-human/mouse/rat Phospho-MEK1
(S218/S222)/MEK2 (S222/S226) polyclonal antibody
(Catalog # AF2506) and anti-human/mouse/rat
MEK1/MEK2 monoclonal antibody (Catalog # MAB2678).
Regulation of Pro- & Anti-Apoptotic Signaling PathwaysIntracellular Kinases
AktANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
AktPan Specificaa 75-90
Human None Polyclonal Rabbit IgG WB AF2055 50 µg
AktPan Specific
Human/Mouse/Rat APC 281046 Mouse IgG2B FC IC2055A 100 Tests
AktPan Specific
Human/Mouse/Rat Fluorescein 281046 Mouse IgG2B FC IC2055F 100 Tests
AktPan Specific
Human/Mouse/Rat PE 281046 Mouse IgG2B FC IC2055P 100 Tests
AktPan Specific
Human/Mouse/Rat None 281046 Mouse IgG2B FC, IHC, WB MAB2055 100 µg
Phospho-Akt (S473) Human/Mouse/Rat None Polyclonal Rabbit IgG FC, IHC, WB AF887 100 µg
Phospho-Akt (S473) Human/Mouse/Rat Fluorescein Polyclonal Rabbit IgG FC IC887F 100 Tests
Phospho-Akt (S473) Human/Mouse/Rat PE Polyclonal Rabbit IgG FC IC887P 100 Tests
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Phospho-Akt (S473)Pan SpecificSurveyor IC ELISA
Human/Mouse/Rat � Cell Lysate N/A 0.31-20 pg/mL SUV887 1 Kit
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-Akt (S473)Pan Specific
Human/Mouse/Rat 312 - 20000 pg/mL DYC887-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
CELL-BASED ASSAYS
ANALYTE SPECIES CATALOG # SIZE
Phospho-Akt (S473)Pan SpecificCell-Based ELISA
Human/Mouse/Rat KCB887 1 Kit
Akt1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Akt1Active
Human Solution without a carrier protein None Sf 9 1775-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Akt1 Human APC 302407 Mouse IgG1 FC IC17751A 100 Tests
Akt1 Human Fluorescein 302407 Mouse IgG1 FC IC17751F 100 Tests
Akt1 Human PE 302407 Mouse IgG1 FC IC17751P 100 Tests
Akt1 Human None 302407 Mouse IgG1 FC, IHC MAB17751 100 µg
Akt1 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1775 50 µg
Akt1 Human/Mouse/Rat None 232790 Mouse IgG2A WB MAB1775 100 µg
Akt1aa 1-149
Human/Mouse/Rat None Polyclonal Sheep IgG WB AF17751 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037384
Akt1 continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-Akt1 (S473) Human/Mouse 312 - 20000 pg/mL DYC2289B-2 2 Plates
Akt1Total Protein
Human/Mouse/Rat 312 - 20000 pg/mL DYC1775-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
Akt2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Akt2 Human APC 302501 Mouse IgG1 FC IC23152A 100 Tests
Akt2 Human PE 302501 Mouse IgG1 FC IC23152P 100 Tests
Akt2 Human None 302501 Mouse IgG1 FC, IHC MAB23152 100 µg
Akt2 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF2315 50 µg
Akt2 Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF23151 100 µg
Akt2 Human/Mouse/Rat None 302514 Mouse IgG3 WB MAB2315 100 µg
Akt3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Akt3 Human None 199864 Mouse IgG1 WB MAB1463 100 µg
Akt3 Human None 199822 Mouse IgG1 FC MAB14631 100 µg
ASK1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
ASK1Activeaa 649-946
Human Solution without a carrier protein None Sf 9 3575-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
ASK1aa 1011-1196
Human None Polyclonal Sheep IgG IHC, WB AF3575 100 µg
CaMKK�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CaMKK�aa 1-132
Human/Rat None Polyclonal Sheep IgG WB AF4536 100 µg
ERK1/ERK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
ERK1/ERK2 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1576 100 µg
ERK1/ERK2 Human/Mouse/Rat None 216703 Mouse IgG1 IHC, WB MAB1576 100 µg
Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1018 100 µg
Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)
Human/Mouse/Rat None 269434 Rabbit IgG WB MAB1018 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 85
For antibody application key, see page 120.
Intracellular Kinases
ERK1/ERK2 continuedELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)Surveyor IC ELISA
Human/Mouse/Rat � Cell Lysate N/A 312.5-20000 pg/mL SUV1018 1 Kit
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)
Human/Mouse/Rat 312 - 20000 pg/mL DYC1018-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
CELL-BASED ASSAYS
ANALYTE SPECIES CATALOG # SIZE
Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)Cell-Based ELISA
Human/Mouse/Rat KCB1018 1 Kit
ERK1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
ERK1Active
Human Solution without a carrier protein None Sf 9 1879-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
ERK1 Human None Polyclonal Goat IgG WB AF1879 100 µg
ERK1 Human Biotin Polyclonal Goat IgG WB BAF1879 50 µg
ERK1aa 1-28
Human None Polyclonal Rabbit IgG WB AF1940 50 µg
ERK1aa 1-28
Human None 250603 Mouse IgG2A IHC, WB MAB1940 100 µg
ERK1 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1575 50 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
ERK1Total Protein
Human 312 - 20000 pg/mL DYC1940-2 2 Plates
Phospho-ERK1 (T202/Y204) Human/Mouse/Rat 125 - 8000 pg/mL DYC1825-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
ERK2RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
ERK2Active
Human Solution without a carrier protein None E. coli 1230-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
ERK2 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1230 100 µg
ERK2 Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF12301 100 µg
ERK2 Human/Mouse/Rat None 191801 Mouse IgG2A IHC, WB MAB1230 100 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Phospho-ERK2 (T185/Y187)Surveyor IC ELISA
Human/Mouse/Rat N/A 0.19-12 ng/mL SUV1483 1 Kit
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037386
ERK2 continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
ERK2Total Protein
Human/Mouse/Rat 312.5 - 20000 pg/mL DYC1230B-2 2 Plates
Phospho-ERK2 (T185/Y187) Human/Mouse/Rat 187 - 12000 pg/mL DYC1483-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
G�LANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
G�L Human/Mouse/Rat None Polyclonal Goat IgG WB AF4004 100 µg
GSK-3�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GSK-3�aa 90-483
Human None 288901 Mouse IgG2B IHC MAB2986 100 µg
Phospho-GSK-3� (S21) Human/Mouse/Rat None Polyclonal Rabbit IgG2B IHC, WB AF4125 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-GSK-3� (S21) Human 156.25 to 10,000 pg/mL DYC4125-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
GSK-3�/�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GSK-3�/� Human/Mouse/Rat None Polyclonal Rabbit IgG FC, WB AF2157 50 µg
Phospho-GSK-3�/� (S21/S9) Human/Mouse/Rat None Polyclonal Rabbit IgG FC, IHC, WB AF1590 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
GSK-3�/�Total Protein
Human/Mouse/Rat 312 - 20000 pg/mL DYC2157-2 2 Plates
Phospho-GSK-3�/� (S21/S9) Human/Mouse/Rat 156 - 10000 pg/mL DYC2630-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
GSK-3�RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
GSK-3�Active
Human Solution without a carrier protein None Sf 9 2506-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
GSK-3� Human Fluorescein 272536 Rat IgG2A FC IC2506F 100 Tests
GSK-3� Human PE 272536 Rat IgG2A FC IC2506P 100 Tests
GSK-3� Human None 272535 Rat IgG2B IHC MAB25061 100 µg
GSK-3� Human/Mouse/Rat None 272536 Rat IgG2A FC, WB MAB2506 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-GSK-3� (S9) Human/Mouse/Rat 312 - 20000 pg/mL DYC1590-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy pack . Standard DuoSet Kits are also available in 45 plate Economy Pack .
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 87
For antibody application key, see page 120.
Intracellular Kinases
ss
Jak1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-Jak1 (Y1022/Y1023) Human None Polyclonal Rabbit IgG WB AF4510 100 µg
Jak1aa 20-274 (human)
Human/Mouse/Rat None 413104 Rat IgG2B FC, IHC, WB MAB4260 100 µg
Jak1aa 14-55 (mouse)
Mouse/Rat None Polyclonal Goat IgG IHC, WB AF602 100 µg
Jak2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Jak2aa 2-63 (mouse)
Mouse/Rat None Polyclonal Goat IgG WB AF2988 100 µg
Jak3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Jak3aa 46-209
Human None 452506 Mouse IgG2B WB MAB4699 100 µg
JNKANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
JNKPan Specific
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1387 100 µg
JNKPan Specific
Human/Mouse/Rat None 252355 Mouse IgG2A WB MAB1387 100 µg
Phospho-JNK (T183/Y185) Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1205 50 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
JNKTotal ProteinPan Specific
Human/Mouse/Rat 625 - 40000 pg/mL DYC1205-2 2 Plates
Phospho-JNK (T183/Y185)Pan Specific
Human/Mouse/Rat 156 - 10000 pg/mL DYC1387-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
JNK1/JNK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
JNK1/JNK2 Human/Mouse/Rat None 252323 Mouse IgG2A IHC, WB MAB2076 100 µg
JNK1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
JNK1Active
Mouse Solution without a carrier protein None Sf 9 1776-KS-010 10 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037388
JNK1 continuedANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
JNK1 Human/Mouse/Rat None 228611 Mouse IgG1 WB MAB1776 100 µg
JNK1 Human/Mouse/Rat None 228601 Mouse IgG1 IHC, WB MAB17761 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
JNK1Total Protein
Human/Mouse/Rat 312 - 20000 pg/mL DYC1776-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
JNK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
JNK2 Human/Mouse/Rat None 252320 Mouse IgG2B IHC, WB MAB1846 100 µg
JNK2aa 267-287
Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1846 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
JNK2Total Protein
Human/Mouse/Rat 156 - 10000 pg/mL DYC1846-2 2 Plates
Phospho-JNK2 (T183/Y185) Human/Mouse/Rat 125 - 8000 pg/mL DYC2236-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
LMTK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
LMTK2 Human None 465222 Mouse IgG2B WB MAB5147 100 µg
MEK1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MEK1 Human/Mouse/Rat None Polyclonal Goat IgG WB AF2079 100 µg
MEK1 Human/Mouse/Rat None 239421 Mouse IgG2A WB MAB2079 100 µg
Phospho-MEK1 (T386) Human/Mouse/Rat/Bovine/Canine/Chicken/Primate/Xenopus
None Polyclonal Rabbit IgG WB PPS076 100 µg
Phospho-MEK1 (T292) Human/Mouse/Rat/Bovine/Canine/Primate
None Polyclonal Rabbit IgG WB PPS075 100 µg
MEK1/MEK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MEK1/MEK2 Human/Mouse/Rat None 239416 Mouse IgG2B IHC, WB MAB2678 100 µg
Phospho-MEK1 (S218/S222)/MEK2 (S222/S226)
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF2506 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-MEK1 (S218/S222)/MEK2 (S222/S226)
Human 312.5 - 20000 pg/mL DYC2506-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy pack . Standard DuoSet Kits are also available in 45 plate Economy Pack .
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 89
For antibody application key, see page 120.
Intracellular Kinases
ss
MEK1/MEK2 continuedCELL-BASED ASSAYS
ANALYTE SPECIES CATALOG # SIZE
Phospho-MEK1 (S218/S222)/MEK2 (S222/S226)Cell-Based ELISA
Human/Mouse/Rat KCB2506 1 Kit
MEK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MEK2 Human None Polyclonal Goat IgG WB AF2855 100 µg
MEK2 Human Biotin Polyclonal Goat IgG WB BAF2855 50 µg
MEK2 Human/Mouse/Rat None 300317 Mouse IgG2A IHC, WB MAB2855 100 µg
MKK3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MKK3 Human/Mouse/Rat None 275909 Mouse IgG2B IHC, WB MAB2515 100 µg
MKK3/MKK6ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-MKK3 (S218/T222)/MKK6 (S207/T211)
Human None Polyclonal Rabbit IgG WB AF4930 250 µg
MKK3/MKK6 Human/Mouse/Rat None 275922 Mouse IgG1 WB MAB2514 100 µg
MKK4ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MKK4 Human None 323042 Mouse IgG2B IHC MAB3390 100 µg
Phospho-MKK4 (S257/T261) Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF2990 100 µg
MKK6RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
MKK6Active
Human Lyophilized without a carrier protein None E. coli 1604-MK-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MKK6 Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1604 50 µg
MKK6 Human/Mouse/Rat None Polyclonal Goat IgG WB AF16041 100 µg
MKK6 Human/Mouse/Rat Biotin Polyclonal Goat IgG WB BAF16041 50 µg
MKK6 Human/Mouse/Rat None 304328 Rat IgG2B IHC, WB MAB1604 100 µg
MKK7ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MKK7 Human None Polyclonal Goat IgG IHC, WB AF3579 100 µg
MKK7 Human None 324302 Mouse IgG2B IHC MAB3579 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037390
MLK3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MLK3aa 85-207
Human None Polyclonal Sheep IgG WB AF4559 100 µg
MLK4�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MLK4�aa 560-570
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF3435 100 µg
MSK1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MSK1 Human None 252608 Mouse IgG2B IHC MAB2518 100 µg
Phospho-MSK1 (S212) Human None Polyclonal Rabbit IgG IHC, WB AF1036 100 µg
MSK1 Human/Mouse None Polyclonal Goat IgG IHC, WB AF2518 100 µg
MSK1/MSK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-MSK1 (S376)/MSK2 (S360)
Human None Polyclonal Rabbit IgG WB AF1094 100 µg
MSK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MSK2 Human/Mouse None 261034 Rat IgG2A WB MAB2310 100 µg
Phospho-MSK2 (S196) Human/Mouse None Polyclonal Rabbit IgG IHC, WB AF189 100 µg
MST1/STK4ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MST1/STK4 Human None Polyclonal Goat IgG WB AF4939 100 µg
MST2/STK3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MST2/STK3aa 339-456
Human/Mouse/Rat None Polyclonal Goat IgG WB AF4549 100 µg
p38ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-p38 (T180/Y182) Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF869 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 91
For antibody application key, see page 120.
Intracellular Kinases
p38 continuedCELL-BASED ASSAYS
ANALYTE SPECIES CATALOG # SIZE
Phospho-p38 (T180/Y182)Cell-Based ELISA
Human/Mouse KCB869 1 Kit
p38�RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
p38�Active
Human Solution without a carrier protein None E. coli 1806-P3-010 10 µg
p38�Unactive
Human Solution without a carrier protein None E. coli 869-P3-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p38� Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF8691 100 µg
p38� Human/Mouse/Rat None 142102 Mouse IgG2B IHC, WB MAB869 100 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Phospho-p38� (T180/Y182)Surveyor IC ELISA
Human/Mouse/Rat � Cell Lysate N/A 62.5-4000 pg/mL SUV869 1 Kit
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
p38�Total Protein
Human/Mouse/Rat 312 - 20000 pg/mL DYC8691B-2 2 Plates
Phospho-p38� (T180/Y182) Human/Mouse/Rat 62.5 - 4000 pg/mL DYC869-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
p38�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p38� Human None 280071 Mouse IgG2B IHC MAB3274 100 µg
p38ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p38 Human None Polyclonal Rabbit IgG IHC, WB AF1519 100 µg
p38 Human None 197202 Mouse IgG1 WB MAB1519 500 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
p38Total Protein
Human 625 - 40000 pg/mL DYC1519-2 2 Plates
Phospho-p38 (T180/Y182) Human 156 - 10000 pg/mL DYC2124-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037392
p38ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p38 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1347 100 µg
p38 Human/Mouse/Rat None Polyclonal Goat IgG WB AF1644 100 µg
p38 Human/Mouse/Rat None 212464 Mouse IgG1 WB MAB1347 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-p38 (T183/Y185) Human/Mouse 125 - 8000 pg/mL DYC1664-2 2 Plates
p38Total Protein
Human/Mouse/Rat 125 - 8000 pg/mL DYC1347-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
p70 S6 KinaseRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
p70 S6 KinaseActive
Human Solution without a carrier protein None Sf 9 896-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-p70 S6 Kinase (T229) Human None Polyclonal Goat IgG IHC, WB AF896 100 µg
Phospho-p70 S6 Kinase (T229) Human None Polyclonal Rabbit IgG WB AF8961 100 µg
Phospho-p70 S6 Kinase (T389) Human None Polyclonal Rabbit IgG WB AF8963 100 µg
p70 S6 Kinase Human/Mouse/Rat None Polyclonal Rabbit IgG FC, IHC, WB AF8962 100 µg
p70 S6 Kinase Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF8964 100 µg
p70 S6 Kinase Human/Mouse/Rat None 215247 Mouse IgG1 FC, IHC, WB MAB8962 100 µg
Phospho-p70 S6 Kinase(T421/S424)
Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF8965 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-p70 S6 Kinase (T389) Human 312 - 20000 pg/mL DYC896-2 2 Plates
p70 S6 KinaseTotal Protein
Human/Mouse/Rat 15.6 - 1000 pg/mL DYC8962-2 2 Plates
Phospho-p70 S6 Kinase(T421/S424)
Human/Mouse/Rat 156 - 10000 pg/mL DYC8965-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
CELL-BASED ASSAYS
ANALYTE SPECIES CATALOG # SIZE
Phospho-p70 S6 Kinase (T389)Cell-Based ELISA
Human/Mouse KCB8963 1 Kit
p70 S6 Kinase�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p70 S6 Kinase� Human None 303421 Mouse IgG2B WB MAB2987 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 93
For antibody application key, see page 120.
Intracellular Kinases
PDK-1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PDK-1aa 296-314
Human None Polyclonal Rabbit IgG WB AF864 100 µg
PI 3-Kinase p85�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PI 3-Kinase p85�aa 328-431
Human/Mouse/Rat None Polyclonal Goat IgG WB AF2998 100 µg
PI 3-Kinase p110ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PI 3-Kinase p110 Human/Mouse/Rat None 376315 Mouse IgG1 WB MAB4426 100 µg
PI 3-Kinase p110�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PI 3-Kinase p110�aa 153-349
Human None 269020 Mouse IgG2B WB MAB2686 100 µg
PI 3-Kinase p110ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PI 3-Kinase p110aa 391-542
Human None 275144 Mouse IgG2B WB MAB2687 100 µg
PI 3-Kinase p110ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PI 3-Kinase p110aa 298-467
Human None 304103 Rat IgG2A WB MAB2999 100 µg
PKA CANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PKA CPan Specific
Human/Mouse/Rat None Polyclonal Goat IgG WB AF4175 100 µg
PKA C�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PKA C� Human/Mouse/Rat None Polyclonal Goat IgG WB AF4268 100 µg
PKA RI�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PKA RI� Human/Mouse/Rat None Polyclonal Sheep IgG WB AF4177 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037394
RictorANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Rictoraa 1-250
Human None Polyclonal Sheep IgG WB AF4598 100 µg
RSKANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-RSK (S380)Pan Specific
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF889 100 µg
Phospho-RSK (T573)Pan Specific
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF8891 100 µg
RSKPan Specificaa 171-193
Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF2056 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-RSK (S221)Pan Specific
Human/Mouse/Rat 156 - 10000 pg/mL DYC3408-2 2 Plates
Phospho-RSK (S380)Pan Specific
Human/Mouse/Rat 156 - 10000 pg/mL DYC889-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
RSK1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
RSK1Active
Human Solution without a carrier protein None Sf 9 992-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RSK1 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF992 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-RSK1 (S380) Human/Mouse/Rat 125 - 8000 pg/mL DYC892-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
RSK1/RSK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-RSK1 (S221)/RSK2 (S227)
Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF892 100 µg
RSK2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RSK2 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1518 50 µg
RSK3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-RSK3 (S218) Human/Mouse None Polyclonal Rabbit IgG IHC, WB AF893 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 95
For antibody application key, see page 120.
Intracellular Kinases
RSK4ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RSK4 Human None 374521 Rat IgG2A WB MAB3916 100 µg
SrcRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
SrcActive
Viral Solution without a carrier protein None Sf 9 3389-KS-010 10 µg
SrcActive
Human Solution without a carrier protein None E. coli 4595-KS-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-Src (T419) Human None Polyclonal Rabbit IgG IHC, WB AF2685 100 µg
Src Human None 327554 Mouse IgG2A IHC MAB3807 100 µg
Srcaa 1-79
Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF3389 100 µg
Srcaa 1-79
Human/Mouse/Rat None 327537 Mouse IgG2A WB MAB3389 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-Src (Y419) Human 3.9 - 250 pg/mL DYC2685-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
TORANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-TOR (S2448) Human None Polyclonal Rabbit IgG WB AF1665 100 µg
TOR Human/Mouse None 303728 Rat IgG2A IHC MAB1537 100 µg
TORaa 1223-1290 (rat)
Human/Mouse/Rat None Polyclonal Total Rabbit IgG WB AB1537 100 µg
TORaa 1720-2020 (human)
Human/Mouse/Rat None Polyclonal Goat IgG IP, WB AF15371 100 µg
ELISAs/ASSAYS
ANALYTE SPECIES SAMPLE TYPES SENSITIVITY RANGE CATALOG # SIZE
Phospho-TOR (S2448)Surveyor IC ELISA
Human � Cell Lysate N/A 156-10000 pg/mL SUV1665 1 Kit
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-TOR (S2448) Human 156 - 10000 pg/mL DYC1665-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
Tyk2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Tyk2aa 2-51
Human None 307214 Mouse IgG3 WB MAB3427 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037396
NF�B Pathway
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 97
NF�B1 and NF�B2 are members of the Rel/NF�B family of tran-
scription factors that also includes RelA, c-Rel, and RelB. Rel/NF�B
members regulate the expression of genes that participate in im-
mune, apoptotic and oncogenic processes. NF�B is predominantly
localized in the cytoplasm as a complex with inhibitory I�B pro-
teins and is released and translocated to the nucleus after
phosphorylation of I�B. Both NF�B1 (105 kDa) and NF�B2
(100 kDa) are synthesized as precursor molecules that are
proteolytically cleaved to 50 and 52 kDa active subunits. NF�B ap-
pears to have contradictory functions in apoptosis and cell survival.
NF�B mediates the survival response of many signals by inhibiting
p53-dependent apoptosis, and up-regulating anti-apoptotic mem-
bers of the Bcl-2 family and caspase inhibitors, such as XIAP and
FLIP. In contrast, NF�B is also activated by apoptotic stimuli in-
volved in DNA damage and mediates up-regulation of
pro-apoptotic genes such as TRAIL R2/DR5, Fas, and Fas ligand.
For more information on NFκB-related products, please visit our
website at www.RnDSystems.com/go/NFkB
NF�B Promotes the Expression of Pro- and Anti-apoptotic Genes. In addition to the recruitment of FADD and Pro-caspase-8, -10, the TRADD adaptor protein can recruit
TRAF-2 leading to the activation of the NF�B transcription factor. NF�B activates the expression of both pro- and anti-apoptotic genes.
Detection of NF�B2 in Daudi Cells. Exponentially growing human Burkitt's lymphoma Daudi cells were either unstimulated (-)
or stimulated (+) with recombinant human CD40 Ligand (Catalog # 617-CL) for 4 hours. NF�B2 was detected in cytoplasmic and
nuclear extracts by Western blot analysis using anti-human NF�B2 monoclonal antibody (Catalog # MAB28881). A whole cell ex-
tract from Burkitt's lymphoma-derived Raji cells is shown as a control in lane 5.
NF�B
c-RelANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
c-Rel Human None 321422 Rat IgG2A IHC, WB MAB4606 50 µg
c-Relaa 1-588
Human/Mouse None Polyclonal Goat IgG IHC, WB AF2699 100 µg
c-Relaa 1-588
Mouse Biotin 290512 Rat IgG2A IHC BAM2699 100 µg
c-Relaa 1-588
Mouse None 290512 Rat IgG2A WB MAB2699 100 µg
CARMA1/CARD11ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CARMA1/CARD11aa 256-435
Human None Polyclonal Goat IgG WB AF4839 100 µg
I�B-�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
I�B-� Human None 417208 Mouse IgG1 WB MAB4299 50 µg
Phospho-I�B-� (S32/S36) Human None Polyclonal Rabbit IgG WB AF4809 100 µg
I�B-� Human/Mouse None Polyclonal Sheep IgG WB AF4299 100 µg
I�B-�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
I�B-� Human/Rat None 347428 Mouse IgG1 WB MAB3425 100 µg
I�B-�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
I�B-�aa 141-500
Human None 418515 Mouse IgG2B WB MAB4300 100 µg
I�B-� Mouse None Polyclonal Sheep IgG WB AF4637 100 µg
IKK�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
IKK�aa 375-745
Human/Mouse/Rat None Polyclonal Sheep IgG IHC, WB AF3768 100 µg
IKK�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
IKK�aa 374-756
Human None Polyclonal Sheep IgG WB AF4535 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 5238037398
IKK�ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
IKK� Human None Polyclonal Goat IgG IHC, WB AF3199 100 µg
IKK� Human/Mouse/Rat None 346909 Mouse IgG1 IHC, WB MAB3199 100 µg
IKKANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
IKK Human/Mouse/Rat None Polyclonal Goat IgG IHC, WB AF2684 100 µg
IKK Human/Mouse/Rat None Polyclonal Sheep IgG WB AF4365 100 µg
NF�B1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
NF�B1aa 317-437 (human)
Human/Mouse None Polyclonal Goat IgG WB AF2697 100 µg
NF�B1aa 317-437 (human)
Human/Mouse None 285412 Mouse IgG1 WB MAB2697 100 µg
NF�B2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
NF�B2aa 1-447
Human None 291319 Mouse IgG1 IHC, WB MAB28881 100 µg
Pin1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Pin1WW Domainaa 2-163HRP-conjugated
Human Solution with a carrier protein HRP E. coli HRP2294 250 µL
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Pin1aa 2-163 (human)
Human/Mouse None 257417 Mouse IgG2B IHC, IP, WB MAB2294 100 µg
RelBANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RelBaa 382-579
Human None 315206 Mouse IgG2B IHC, WB MAB2698 100 µg
SUMO1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SUMO1 Human None Polyclonal Goat IgG WB AF3289 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 99
For antibody application key, see page 120.
NF�B
SUMO2/3/4ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SUMO2/3/4 Human/Mouse None Polyclonal Goat IgG WB AF3020 100 µg
SUMO3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
SUMO3 Human/Mouse None Polyclonal Goat IgG WB AF2959 100 µg
UbiquitinRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Ubiquitin Human Solution without a carrier protein None E. coli 701-UB-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Ubiquitin/Ubiquitin+1 Human None 83406 Mouse IgG2B IHC, WB MAB701 100 µg
Ubiquitin+1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Ubiquitin+1 Human Solution without a carrier protein None E. coli 703-UB-025 25 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Ubiquitin+1 Human None Polyclonal Rabbit IgG WB AF703 100 µg
Ubiquitin+1 Human Biotin 83426 Mouse IgG2B ELISA(Det), WB BAM7032 250 µg
Ubiquitin+1 Human None 82001 Mouse IgG2A IHC, WB MAB703 100 µg
Ubiquitin+1 Human None 83456 Mouse IgG2B ELISA(Cap), WB MAB7031 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Ubiquitin+1 Human 125 - 8000 pg/mL DY703 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy pack . Standard DuoSet Kits are also available in 45 plate Economy Pack .
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373100
ss
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 101
p53 is a Key Regulator in Determining the Fate of a Cell following DNA Damage. Under normal conditions, p53 is ubiquitinated by MDM2 and rapidly degraded by the
proteasome. When DNA is damaged, p53 phosphorylation stabilizes the protein by preventing its interaction with MDM2. Phosphorylated p53 can promote cell cycle arrest
and DNA repair, or apoptotic signaling pathways.
The p53 tumor suppressor protein is a multi-functional transcrip-
tion factor that regulates cellular processes affecting proliferation,
cell cycle checkpoints, and apoptosis. Binding of MDM2 regulates
p53 activity by targeting p53 for degradation and inhibits
p53-induced cell cycle arrest and apoptosis. In response to
genotoxic stress signals or DNA damage, p53 is phosphorylated, in-
hibiting its interaction with MDM2. p53 can cause cell cycle arrest
to allow for DNA damage repair or can cause the cell to undergo
apoptosis if the damage cannot be repaired. In addition, p53 has
been shown to trigger apoptotic pathways independent of its ac-
tivity as a transcription factor.
For more information on p53-related products, please visit our website
at www.RnDSystems.com/go/p53
Detection of Phosphorylated p53 in CPT-treated
Human MCF-7 Cells. Human MCF-7 breast cancer cells
were left untreated or treated with camptothecin (CPT;
1 µM) for the indicated times. A portion of the six hour
CPT-treated sample was subsequently treated with
�-phosphatase (�-PPase). Cell lysates were assessed for
p53 phosphorylation on serine 46 using the human
Phospho-p53 (S46) DuoSet® IC ELISA Development System
(Catalog # DYC1489; histogram). The same lysates were
also analyzed by Western blot (inset) using anti-human
Phospho-p53 (S46) polyclonal antibody (Catalog #
AF1489) and HRP-conjugated anti-human/mouse/rat
p53 polyclonal antibody (Catalog # HAF1355).
Dete c ti o n o f Phos ph o r ylate d p53 (S15) i n
CPT- treate d Os teo s arco ma Ce lls. Human
osteosarcoma cells were treated with camptothecin
(1µM; 4 hours). p53 phosphorylation on S15 was de-
tected using anti-human Phospho-p53 (S15) polyclonal
antibody (Catalog # AF1043). Cells were stained with a
FITC-conjugated secondary antibody (green) and
counterstained with propidium iodide (red).
Detection of p53 Binding to the p21 Promoter by
Chromatin Immunoprecipitation. Human MCF-7
breast cancer cells were treated with camptothecin
(300nM), fixed, and lysed. p53 binding to the p21 pro-
moter was assessed using the p53 ExactaChIP™
Chromatin IP Kit (Catalog # ECP1355). Briefly, cell lysates
were incubated with biotinylated anti-human p53
polyclonal antibody or biotinylated anti-goat IgG
polyclonal antibody (both provided in the kit) followed
by MagCellect Streptavidin Ferrofluid (Catalog #
MAG999) . DNA was puri f ied from the
immunoprecipitates and the p21 promoter was de-
tected by standard PCR .
p53 Pathway
p53 Pathway
53BP1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
53BP1 Human None Polyclonal Goat IgG WB AF1877 100 µg
Phospho-53BP1 (S25) Human None Polyclonal Rabbit IgG WB AF3405 100 µg
ATMANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
ATM Human None 245403 Mouse IgG3 WB MAB2290 100 µg
Phospho-ATM (S1981) Human/Mouse/Rat None Polyclonal Rabbit IgG WB AF1655 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-ATM (S1981) Human 3.12 - 200 ng/mL DYC1655-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
CBPANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CBP Human/Mouse/Rat None 257003 Mouse IgG2B IHC, WB MAB2676 100 µg
MDM2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MDM2aa 3-492
Human None 197702 Mouse IgG2B IP MAB1244 100 µg
MDM2 Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB AF1244 100 µg
MDM2 Human/Mouse/Rat Biotin Polyclonal Rabbit IgG WB BAF1244 50 µg
p53ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p53 Human APC 184727 Mouse IgG2B FC IC13551A 100 Tests
Phospho-p53 (S15) Human PE 261352 Mouse IgG1 FC IC1839P 100 Tests
Phospho-p53 (S15) Human None Polyclonal Rabbit IgG IHC, IP, WB AF1043 100 µg
Phospho-p53 (S15) Human None 261352 Mouse IgG1 WB MAB1839 100 µg
Phospho-p53 (S15) Human None 261366 Mouse IgG1 IHC MAB18391 100 µg
Phospho-p53 (S20) Human None Polyclonal Rabbit IgG WB AF2286 100 µg
Phospho-p53 (S37) Human None Polyclonal Rabbit IgG WB AF3306 50 µg
Phospho-p53 (S392) Human None Polyclonal Rabbit IgG WB AF2996 50 µg
Phospho-p53 (S46) Human None Polyclonal Rabbit IgG IHC, WB AF1489 100 µg
p53 Human/Mouse/Rat None Polyclonal Goat IgG IP, WB AF1355 100 µg
p53 Human/Mouse/Rat Biotin Polyclonal Goat IgG WB BAF1355 50 µg
p53 Human/Mouse/Rat Agarose Polyclonal Goat IgG IP GAF1355 250 µL
p53 Human/Mouse/Rat HRP Polyclonal Goat IgG WB HAF1355 100 µL
p53 Human/Mouse/Rat None 184721 Mouse IgG2B IP, WB MAB1355 100 µg
p53 Human/Mouse/Rat None 184727 Mouse IgG2B FC MAB13551 100 µg
p53 Mouse None 197643 Rat IgG2B IP MAB1746 100 µg
Phospho-p53 (S18) Mouse None Polyclonal Rabbit IgG WB AF2887 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373102
p53 continuedDUOSET IC ELISA DEVELOPMENT SYSTEMS & TRANSCRIPTION FACTOR ACTIVITY ASSAYS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
p53Total Protein
Human 125 - 8000 pg/mL DYC1043-2 2 Plates
Phospho-p53 (S15) Human 312 - 20000 pg/mL DYC1839-2 2 Plates
Phospho-p53 (S46) Human 625 - 40000 pg/mL DYC1489-2 2 Plates
P53Transcription Factor Assay
Human/Mouse N/A DYC1355-2 2 Plates
p53Total Protein
Mouse 312 - 20000 pg/mL DYC1746-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
EXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS
ANALYTE SPECIES CATALOG # SIZE
p53 Human ECP1355 1 Kit
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
p53 Human/Rat RDP-144-025 25 Tests
p53R2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p53R2aa 1-180
Human None Polyclonal Goat IgG WB AF3788 100 µg
p300ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p300aa 2283-2414
Human None Polyclonal Goat IgG WB AF3789 100 µg
EXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS
ANALYTE SPECIES CATALOG # SIZE
p300 Human ECP3789 1 Kit
Pin1RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Pin1WW Domainaa 2-163HRP-conjugated
Human Solution with a carrier protein HRP E. coli HRP2294 250 µL
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Pin1aa 2-163
Human/Mouse None 257417 Mouse IgG2B IHC, IP, WB MAB2294 100 µg
RTVP-1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RTVP-1 Mouse None Polyclonal Goat IgG WB AF4468 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 103
For antibody application key, see page 120.
p53 Pathway
WISP-1/CCN4RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
WISP-1/CCN4 Human Lyophilized without a carrier protein None NS0 1627-WS-050 50 µg
WISP-1/CCN4 Mouse Lyophilized without a carrier protein None NS0 1680-WS-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
WISP-1/CCN4 Human None Polyclonal Goat IgG IHC, WB AF1627 100 µg
WISP-1/CCN4 Human Biotin Polyclonal Goat IgG WB BAF1627 50 µg
WISP-1/CCN4 Human None 213611 Mouse IgG2A WB MAB1627 500 µg
WISP-1/CCN4 Mouse None Polyclonal Sheep IgG IHC, WB AF1680 100 µg
WISP-1/CCN4 Mouse Biotin Polyclonal Sheep IgG IHC, WB BAF1680 50 µg
WISP-1/CCN4 Mouse None 214203 Rat IgG2B WB MAB1680 500 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373104
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 105
In addition to NF�B and p53, the STAT family of transcription fac-
tors and Mad, Max, and c-Myc are also involved in regulating
proliferative and pro-apoptotic gene expression. The STAT family
of transcription factors is activated by intracellular kinases such as
Jak (1-3), TyK2, and Src. Upon activation, STATs are free to hetero-
or homo-dimerize and translocate to the nucleus where they pro-
mote the expression of Bcl-2 and Bcl-xL. Mad, Max, and c-Myc
belong to the basic helix-loop-helix/leucine zipper family of tran-
scription factors. Both c-Myc and Mad form heterodimers with
Max to regulate transcription. Mad-Max heterodimers are associated
with transcriptional repression and cellular differentiation, while
Myc-Max heterodimers are associated with transcriptional activa-
tion and cellular proliferation.
For more information on products related to Transcription Factors
i n v o l v e d i n a p o p t o s i s , p l e a s e v i s i t o u r w e b s i t e a t
www.RnDSystems.com/go/ApoptosisTxn
Apoptosis Transcription Factors
Other Transcription Factors Regulating Pro- and Anti-Apoptotic Signaling
Active STAT1 p91 in Human U937 Cells. Human monocytic U937
cells were treated with 100 ng/mL recombinant human IFN-� (Cata-
log # 285-IF) for the indicated times. A. Nuclear extracts were
prepared and assayed using the human/mouse Active STAT1 p91
DuoSet® IC Transcription Factor Assay (Catalog # DYC1490). B. The
same extracts (20 µg) were analyzed by Western blot. Total STAT p91
was immunoprecipitated using anti-human/mouse STAT1 p91
polyclonal antibody (Catalog # PAF-ST1) and immunoblotted using
anti-rabbit Phospho-STAT1 (Y701) polyclonal antibody (Catalog #
AF2894). C. EMSA was performed by incubating the same extracts
with 30 fmol biotinylated double-stranded oligonucleotide followed
by electrophoresis on a 5% non-denaturing polyacrylamide gel.
Detection of STAT5 Binding to the Bcl-x Pro-
moter by Chromatin Immunoprecipitation.
Mouse lymphoid Baf/3 cells were grown in the ab-
sence (-) or presence (+) of IL-3 , fixed, and lysed.
STAT5 binding to the Bcl-x promoter was assessed
using the STAT5 ExactaChIP™ Chromatin IP Kit
(Catalog # ECP2168). Briefly, immunoprecipitation
was performed by incubating cell lysates with
biotinylated anti-human/mouse STAT5 polyclonal
antibody or biotinylated anti-rabbit IgG polyclonal
antibody (both provided in the kit) followed by
MagCellect Streptavidin Ferrofluid (Catalog #
MAG999) . DNA was puri f ied from the
immunoprecipitates and the Bcl-x promoter was
detected by standard PCR. Lane 1 = DNA marker.
Intracellular Detection of STAT3 in Human
Jurkat Cells by Flow Cytometry. Human Jurkat
T cells were stained with PE-conjugated anti-hu-
man STAT3 monoclonal antibody (Catalog #
IC1799P; filled histogram) or with PE-conjugated
mouse IgG2B isotype control antibody (Catalog #
IC0041P; open histogram).
CBPANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CBP Human/Mouse/Rat None 257003 Mouse IgG2B IHC, WB MAB2676 100 µg
CREBANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-CREB (S133) None Polyclonal Rabbit IgG WB AF2510 100 µg
Human CREBaa 136 - 341
Human None Polyclonal Goat IgG IHC, WB AF2989 100 µg
Human CREBaa 136 - 341
Mouse None 321120 Mouse IgG1 IHC MAB2989 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-CREB (S133) Human/Mouse/Rat 31.25-2000 pg/mL DYC2510-2 2 Plates
*DuoSet IC Kits are also available in 5 plate packs and 15 plate Economy Packs.
EXACTAChIP CHROMATIN IP KITS
ANALYTE SPECIES CATALOG # SIZE
CREB Human ECP2989 1 Kit
FosB/G0S3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
FosB/G0S3 Human None Polyclonal Goat IgG IHC, WB AF2214 100 µg
FosB/G0S3 Human Biotin Polyclonal Goat IgG WB BAF2214 50 µg
FosB/G0S3 Human/Mouse None 269928 Mouse IgG2B WB MAB2214 100 µg
JunBANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
JunB Human None Polyclonal Sheep IgG WB AF4456 100 µg
MAD1L1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MAD1L1aa 1-350
Human None Polyclonal Goat IgG WB AF4194 100 µg
MaxANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Maxaa 37-141
Human None Polyclonal Goat IgG WB AF4304 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373106
c-MycANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
c-Mycaa 66-201
Human None Polyclonal Goat IgG IHC, WB AF3696 100 µg
c-Myc Human Biotin Polyclonal Goat IgG WB BAF3696 50 µg
EXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS
ANALYTE SPECIES CATALOG # SIZE
c-Myc Human ECP3696 1 Kit
NF�B1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
NF�B1aa 317-437 (human)
Human/Mouse None Polyclonal Goat IgG WB AF2697 100 µg
NF�B1aa 317-437 (human)
Human/Mouse None 285412 Mouse IgG1 WB MAB2697 100 µg
NF�B2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
NF�B2aa 1-447
Human None 291319 Mouse IgG1 IHC, WB MAB28881 100 µg
p53ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
p53 Human APC 184727 Mouse IgG2B FC IC13551A 100 Tests
Phospho-p53 (S15) Human PE 261352 Mouse IgG1 FC IC1839P 100 Tests
Phospho-p53 (S15) Human None Polyclonal Rabbit IgG IHC, IP, WB AF1043 100 µg
Phospho-p53 (S15) Human None 261352 Mouse IgG1 WB MAB1839 100 µg
Phospho-p53 (S15) Human None 261366 Mouse IgG1 IHC MAB18391 100 µg
Phospho-p53 (S20) Human None Polyclonal Rabbit IgG WB AF2286 100 µg
Phospho-p53 (S37) Human None Polyclonal Rabbit IgG WB AF3306 50 µg
Phospho-p53 (S392) Human None Polyclonal Rabbit IgG WB AF2996 50 µg
Phospho-p53 (S46) Human None Polyclonal Rabbit IgG IHC, WB AF1489 100 µg
p53 Human/Mouse/Rat None Polyclonal Goat IgG IP, WB AF1355 100 µg
p53 Human/Mouse/Rat Biotin Polyclonal Goat IgG WB BAF1355 50 µg
p53 Human/Mouse/Rat Agarose Polyclonal Goat IgG IP GAF1355 250 µL
p53 Human/Mouse/Rat HRP Polyclonal Goat IgG WB HAF1355 100 µL
p53 Human/Mouse/Rat None 184721 Mouse IgG2B IP, WB MAB1355 100 µg
p53 Human/Mouse/Rat None 184727 Mouse IgG2B FC MAB13551 100 µg
p53 Mouse None 197643 Rat IgG2B IP MAB1746 100 µg
Phospho-p53 (S18) Mouse None Polyclonal Rabbit IgG WB AF2887 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 107
For antibody application key, see page 120.
Apoptosis Transcription Factors
p53 continuedDUOSET IC TRANSCRIPTION FACTOR ELISAS AND ACTIVITY ASSAYS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
p53Total Protein
Human 125 - 8000 pg/mL DYC1043-2 2 Plates
Phospho-p53 (S15) Human 312 - 20000 pg/mL DYC1839-2 2 Plates
Phospho-p55 (S46) Human 625 - 40000 pg/mL DYC1489-2 2 Plates
p53Transcription FactorActivity Assay
Human/Mouse N/A DYC1355-2 2 Plates
p53Total Protein
Mouse 312 - 20000 pg/mL DYC1746-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
EXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS
ANALYTE SPECIES CATALOG SIZE
p53 Human ECP1355 1 Kit
PRIMER PAIRS
PRODUCT SPECIES CATALOG # SIZE
p53 Human/Rat RDP-144-025 25 Tests
c-RelANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
c-Rel Human None 321422 Rat IgG2A IHC, WB MAB4606 50 µg
c-Relaa 1-588
Human/Mouse None Polyclonal Goat IgG IHC, WB AF2699 100 µg
c-Relaa 1-588
Mouse Biotin 290512 Rat IgG2A IHC BAM2699 100 µg
c-Relaa 1-588
Mouse None 290512 Rat IgG2A WB MAB2699 100 µg
RelBANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
RelBaa 382-579
Human None 315206 Mouse IgG2B IHC, WB MAB2698 100 µg
STAT1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
STAT1aa 686-750 (human)
Human Biotin Polyclonal Goat IgG ELISA(Det) BAF1490 50 µg
Phospho-STAT1 (Y701) Human/Mouse None Polyclonal Rabbit IgG FC, WB AF2894 50 µg
STAT1aa 702-739 (human)
Human/Mouse None Polyclonal Goat IgG IP, WB PAF-ST1 100 µg
DUOSET IC TRANSCRIPTION FACTOR ACTIVITY ASSAYS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
STAT1Transcription FactorActivity Assay
Human/Mouse N/A DYC1490-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373108
For antibody application key, see page 120.
STAT2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-STAT2 Human Fluorescein Polyclonal Rabbit IgG FC IC2890F 100 Tests
STAT2aa 679-851
Human Biotin Polyclonal Goat IgG ELISA(Det) BAF1666 50 µg
STAT2aa 679-851
Human None Polyclonal Goat IgG IP, WB PAF-ST2 100 µg
Phospho-STAT2 (Y689) Human/Mouse None Polyclonal Rabbit IgG FC, WB AF2890 50 µg
DUOSET IC TRANSCRIPTION FACTOR ACTIVITY ASSAYS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
STAT2Transcription FactorActivity Assay
Human N/A DYC1666-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
STAT3ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-STAT3 (Y705) Human None Polyclonal Rabbit IgG WB AF4607 100 µg
STAT3 Human PE 232209 Mouse IgG2B FC IC1799P 100 Tests
STAT3aa 1-175
Human None Polyclonal Sheep IgG WB AF17991 100 µg
STAT3aa 1-175
Human Fluorescein 232209 Mouse IgG2B FC IC1799F 100 Tests
STAT3aa 1-175
Human None 232209 Mouse IgG2B FC, IHC, IP, WB MAB1799 100 µg
STAT3aa 688-770 (mouse)
Human/Mouse/Rat None Polyclonal Goat IgG IHC, IP AF1799 100 µg
DUOSET IC ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Phospho-STAT3 (Y705) Human/Mouse N/A DYC4607-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
EXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS
ANALYTE SPECIES CATALOG # SIZE
STAT3 Human/Mouse ECP1799 1 Kit
STAT4ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-STAT4 (Y693) Human None Polyclonal Rabbit IgG WB AF4319 100 µg
STAT4aa 675-749 (mouse)
Human/Mouse Biotin Polyclonal Goat IgG ELISA(Det) BAF1574 50 µg
STAT4aa 730-749 (mouse)
Human/Mouse None Polyclonal Goat IgG IP, WB PAF-ST4 100 µg
STAT4aa 730-749 (mouse)
Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST4 200 µL
DUOSET IC TRANSCRIPTION FACTOR ACTIVITY ASSAYS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
STAT4Transcription FactorActivity Assay
Human N/A DYC1574-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
STAT5a/bANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-STAT5a/b (Y699) Human None Polyclonal Rabbit IgG WB AF4190 100 µg
STAT5a/baa 219-390 (human)
Human/Mouse None Polyclonal Rabbit IgG WB AF2168 100 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 109
Apoptosis Transcription Factors
STAT5a/b continuedEXACTAChIP CHROMATIN IMMUNOPRECIPITATION KITS
ANALYTE SPECIES CATALOG # SIZE
STAT5a/b Human/Mouse ECP2168 1 Kit
STAT5aANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
STAT5a Human Fluorescein 251610 Mouse IgG1 FC IC21741F 100 Tests
STAT5aaa 774-794
Human PE 251610 Mouse IgG1 FC IC21741P 100 Tests
STAT5aaa 774-794
Human None 251610 Mouse IgG1 FC, IHC MAB21741 100 µg
STAT5aaa 774-794 (human)
Human/Mouse None 251619 Mouse IgG3 IHC, WB MAB2174 100 µg
STAT5aaa 775-794 (mouse)
Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST5A 200 µL
STAT5bANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
STAT5baa 777-787
Human None 389215 Mouse IgG2B FC, WB MAB1584 100 µg
STAT5baa 769-782 (human)
Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST5B 200 µL
STAT5baa 777-787 (human)
Human/Mouse None Polyclonal Rabbit IgG FC, IP, WB AF1584 50 µg
STAT6ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Phospho-STAT6 (Y641) Human None Polyclonal Rabbit IgG FC, WB AF3717 50 µg
Phospho-STAT6 (Y641) Human APC Polyclonal Rabbit IgG FC IC3717A 100 Tests
Phospho-STAT6 (Y641) Human Fluorescein Polyclonal Rabbit IgG FC IC3717F 100 Tests
Phospho-STAT6 (Y641) Human PE Polyclonal Rabbit IgG FC IC3717P 100 Tests
STAT6aa 342-640
Human APC 253906 Mouse IgG2A FC IC2167A 100 Tests
STAT6aa 342-640
Human Fluorescein 253906 Mouse IgG2A FC IC2167F 100 Tests
STAT6aa 342-640
Human PE 253906 Mouse IgG2A FC IC2167P 100 Tests
STAT6aa 342-640 (human)
Human None 253906 Mouse IgG2A FC, WB MAB2167 100 µg
STAT6aa 342-640 (human)
Human/Mouse None Polyclonal Rabbit IgG WB AF21671 50 µg
STAT6aa 818-837 (human)
Human/Mouse None Polyclonal Rabbit Serum IP, WB PA-ST6 200 µL
STAT6aa 627-838 (mouse)
Human/Mouse/Rat None Polyclonal Goat IgG FC, WB AF2167 100 µg
STAT6aa 627-837
Mouse None 69727 Rat IgG2A WB MAB2169 100 µg
DUOSET IC TRANSCRIPTION FACTOR ACTIVITY ASSAYS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
STAT6Transcription FactorActivity Assay
Mouse N/A DYC2169-2 2 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
CELL-BASED ASSAYS
ANALYTE SPECIES CATALOG # SIZE
Phospho-STAT6 (Y641)Cell-Based ELISA
Human/Mouse KCB3717 1 Kit
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
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Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 111
For antibody application key, see page 120.
Cytolytic Granule Proteins
Cytotoxic cells, such as cytotoxic T lymphocytes (CTLs) and natural
killer (NK) cells, play critical roles in the immune response to viruses
and cellular transformation. This results from their ability to recog-
nize and initiate apoptosis in affected cells. One mechanism
cytotoxic cells utilize is the release of lysosomal granules containing
proteins capable of entering the cytoplasm of the target cell and
initiating cascades of events that ultimately result in its controlled
destruction. These include the granzymes, a family of serine pro-
teases that exhibit differences in substrate specificity and
species-specific expression patterns. Several are capable of initiat-
ing apoptotic cascades by caspase-dependent and independent
mechanisms. Granulysin is a granule protein known for its actions
as an anti-microbial agent and may also have a direct cytotoxic ef-
fect on mammalian cells. Other granule-associated proteins include
additional apoptosis mediators, proteins responsible for activating
pro-enzymes, and those with putative involvement in protecting
the effector cell from self destruction (Table 1).
Protein Function
Granzymes Serine proteases with roles in caspase-dependent and-independent apoptosis
Perforin Membrane perturbing protein important for granzymeentry into target cell cytoplasm
Calreticulin Binds Perforin and may inhibit Perforin-mediateddamage to the effector cell
Cathepsin B Appears on the cell surface following degranulation andmay offer protection from Perforin-mediatedself-destruction
Cathepsin C Processes granzyme pro-enzymes
Granulysin Human membrane perturbing microbicidal protein thatcan initiate cytochrome c release and apoptosis
Serglycin Proteoglycan that non-covalently binds granzymes andmay act as a molecular chaperone
Chemokines Small proteins capable of mediating chemotaxis and/orcell activation
LAMPs Protection of lysosomal membranes from enzymaticattack or mediate fusion of phagosomes with lysosomes
WIP Cytolytic granule transport
FAS Ligand/TNFSF6 Potent apoptosis-inducing TNF superfamily member
Granzymes are structurally related serine proteases that differ in
their substrate specificity. To date, five different granzymes have
been described in humans: Granzymes A, B, H, K and M. In
mice, clear orthologues of four of these Granzymes (A, B, K and M)
can be found, and Granzyme C seems the most probable murine
orthologue of Granzyme H. The murine genome encodes several
additional orphan Granzymes (D, E, F, G, L and N). Of these, D, E,
F, and G are expressed by cytotoxic lymphocytes, but not much
else, other than their proposed specificities, is known about them.
Their ubiquitous expression in cytotoxic lymphocytes suggests
that, like the more well-characterized granzymes, these orphan
granzymes also play a role in host protection.
The pathways by which different granzymes carry out cell death are
widely varied. Table 2 shows a description of the activity of the
granzymes for which a function in cytotoxicity has been reported.
For more information on Granzyme-related products, please visit our
website at www.RnDSystems.com/go/Granzymes
Table 1. Cytolytic granule contents and their functions
Table 2. Activities and Mechanisms of Cell Death Induced by Cytotoxic Granzymes
Cytotoxic Granule Proteins
Granzyme-mediated Cell Death
Granzyme A B H/C K M
Activity Tryptase Chymase Chymase Tryptase Metase
Predicted Specificities Arg/Lys/Phe Asp/Asn/Met/Ser Phe/Leu Arg/Lys/Phe Met/Leu
Chromatin Condensation Yes Yes Yes Yes Yes
DNA Fragmentation No Yes No No No
DNA Nicks Yes No Yes Yes Not Determined
Caspase Dependent No Yes No No No
Inhibition by Bcl-2 No Yes Not Determined Yes No
Cytochrome c Release No Yes Yes No No
Mitochondrial Swelling Yes Yes Yes Yes Yes
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Granzyme-mediated Cell Death Involves Caspase-dependent and Caspase-independent Pathways. Cytotoxic cells release cytolytic granules containing proteins such as
Perforin, and granzymes that can enter the cytoplasm of target cells and promote caspase-dependent, or caspase-independent cell death signaling pathways. Granzyme A in-
duces caspase-independent cell death by targeting components of the SET complex which release NM23-H1 to elicit single-stranded DNA nicks. Other Granzyme A substrates
include the core and H1 histones and the nuclear lamins. Granzyme B directly targets Pro-caspase-3 and Pro-caspase-7 to activate apoptotic cell death. In addition, Granzyme B
targets BID leading to the release of Cytochrome c from the mitochondria and the subsequent formation of the apoptosome. Granzyme B may also directly target Lamin B, PARP,
and ICAD, and other cellular substrates to promote cell death.
G ra n z ym e A in H u m a n Pe rip h e ra l B l o o d
Mononuclear Cells. Granzyme A was detected in
human peripheral blood mononuclear cells using
anti-human Granz yme A monoclonal antibody
(Catalog # MAB29051) followed by anti-mouse
NorthernLights™-557 secondary antibody (Catalog #
NL007; red). Cells were counterstained with FluoroNissl™
Green (green).
Intracellular Detection of Granzyme A in CD56+
Whole Blood Cells by Flow Cytometry. CD56+ whole
blood cells were stained with CFS-conjugated anti-hu-
man Granzyme A monoclonal antibody (Catalog #
IC29051F; filled histogram) or with CFS-conjugated
mouse IgG2B isotype control antibody (Catalog #
IC0041F; open histogram).
Detection of Granzyme B-secreting Cells by ELISpot.
Peripheral blood mononuclear cells, stimulated with
calcium ionomycin and PMA, were assessed for
Granzyme B secretion using the human Granzyme B
ELISpot Kit (Catalog # EL2906). Briefly, activated cells
were added to the ELISpot plate for 17 hours followed by
development with biotinylated anti-Granzyme B detec-
tion antibody, and alkaline phosphatase-conjugated
Streptavidin. Granzyme B-secreting cells were revealed
as purple spots in the microplate well. The inset shows a
well in which no detection antibody was added.
Granzyme ARECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Granzyme A Human Lyophilized without a carrier protein None NS0 2905-SE-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Granzyme A Human APC 356412 Mouse IgG2B FC IC29051A 100 Tests
Granzyme A Human Fluorescein 356412 Mouse IgG2B FC IC29051F 100 Tests
Granzyme A Human PE 356412 Mouse IgG2B FC IC29051P 100 Tests
Granzyme A Human None 356422 Mouse IgG1 WB MAB2905 100 µg
Granzyme A Human None 356412 Mouse IgG2B FC, IHC, IP MAB29051 100 µg
Granzyme BRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Granzyme B Human Solution without a carrier protein None NS0 2906-SE-010 10 µg
Granzyme B Mouse Solution without a carrier protein None NS0 1865-SE-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Granzyme B Human None Polyclonal Goat IgG WB AF2906 100 µg
Granzyme B Human Biotin Polyclonal Goat IgG FC, WB BAF2906 50 µg
Granzyme B Human APC 351927 Mouse IgG2A FC IC2906A 100 Tests
Granzyme B Human PE 351927 Mouse IgG2A FC IC2906P 100 Tests
Granzyme B Human None 351927 Mouse IgG2A FC, IHC, WB MAB2906 100 µg
Granzyme B Mouse None Polyclonal Goat IgG B/N, ELISA(Cap), IHC,IP, WB
AF1865 100 µg
Granzyme B Mouse Biotin Polyclonal Goat IgG ELISA(Det), IHC, WB BAF1865 50 µg
Granzyme B Mouse None 216315 Rat IgG2A WB MAB1865 500 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
Granzyme B Mouse 62.5 - 4000 pg/mL DY1865 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy packs. Standard DuoSet Kits are also available in 45 plate Economy Packs.
ELISPOT KITS
ANALYTE SPECIES CATALOG # SIZE**
Granzyme B Human EL2906 1 Kit
Granzyme B Mouse EL1865 1 Kit
** ELISpot Kits are also available in 6 and 50 plate packs and development modules containing enough reagents for 5 plates, please inquire.
Granzyme DRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Granzyme D Mouse Lyophilized without a carrier protein None NS0 1365-SE-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Granzyme D Mouse None Polyclonal Goat IgG IHC, IP, WB AF1365 100 µg
Granzyme D Mouse Biotin Polyclonal Goat IgG WB BAF1365 50 µg
Granzyme D Mouse None 190617 Rat IgG1 WB MAB1365 500 µg
Apoptosis-related Molecule Families
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For antibody application key, see page 120.
Cytotoxic Granule Proteins
Granzyme GRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Granzyme G Mouse Solution without a carrier protein None Sf 21 1346-SE-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Granzyme G Mouse None Polyclonal Goat IgG IP, WB AF1346 100 µg
Granzyme G Mouse Biotin Polyclonal Goat IgG WB BAF1346 50 µg
Granzyme G Mouse None 207639 Rat IgG2A WB MAB1346 500 µg
Granzyme HRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Granzyme H Human Solution without a carrier protein None NS0 1377-SE-010 10 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Granzyme H Human None Polyclonal Goat IgG IHC, IP, WB AF1377 100 µg
Granzyme H Human Biotin Polyclonal Goat IgG IHC, WB BAF1377 50 µg
Granzyme H Human None 185813 Mouse IgG2B WB MAB1377 500 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
.U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373114
Other Apoptosis Molecules
AATKANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
AATK Human None 456006 Mouse IgG2B FC MAB4866 100 µg
Annexin VRECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Annexin V Human Solution without a carrier protein Fluorescein E. coli NX50 100 Tests !
Annexin V Human Solution without a carrier protein PE E. coli NXPE 100 Tests
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Annexin V Human None Polyclonal Goat IgG WB AF399 100 µg
Annexin V Human Biotin Polyclonal Goat IgG WB BAF399 50 µg
For Annexin V apoptosis assays, see Apoptosis Detection Kits section (page 5) at the front of the catalog.
Apolipoprotein H/ApoHANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Apolipoprotein H/ApoH,aa 20-345
Human None Polyclonal Goat IgG WB AF5087 100 µg
C1DANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
C1D Human None Polyclonal Goat IgG WB AF4816 100 µg
CRF21ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CRF21 Human None Polyclonal Sheep IgG WB AF5086 100 µg
CTGF/CCN2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
CTGF/CCN2C-Terminus
Human None Polyclonal Goat IgG IHC, WB AF660 100 µg
CTGF/CCN2C-Terminus
Human Biotin Polyclonal Goat IgG WB BAF660 50 µg
CTGF/CCN2C-Terminus
Human None 88430 Mouse IgG1 IHC MAB660 500 µg
Cyr61/CCN1ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Cyr61/CCN1 Human None 365108 Mouse IgG1 WB MAB4055 100 µg
Cyr61/CCN1 Mouse None Polyclonal Sheep IgG IHC, WB AF4055 100 µg
Cyr61/CCN1 Mouse None 466305 Rat IgG2B WB MAB4864 100 µg
Apoptosis-related Molecule Families
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For antibody application key, see page 120.
Other Apoptosis Molecules
MFG-E8RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
MFG-E8 Human Lyophilized without a carrier protein None NS0 2767-MF-050 50 µg
MFG-E8 Mouse Lyophilized with a carrier protein None NS0 2805-MF-050 50 µg
ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
MFG-E8 Human APC 278918 Mouse IgG2A FC IC27671A 100 Tests
MFG-E8 Human PE 278918 Mouse IgG2A FC IC27671P 100 Tests
MFG-E8 Human None 278901 Mouse IgG2A WB MAB2767 100 µg
MFG-E8 Human None 278918 Mouse IgG2A FC, WB MAB27671 100 µg
MFG-E8 Mouse None Polyclonal Goat IgG WB AF2805 100 µg
MFG-E8 Mouse Biotin Polyclonal Goat IgG ELISA(Det), WB BAF2805 50 µg
MFG-E8 Mouse None 340614 Rat IgG2A ELISA(Cap), WB MAB2805 100 µg
DUOSET ELISA DEVELOPMENT SYSTEMS
ANALYTE SPECIES RECOMMENDED RANGE CATALOG # REAGENTS FOR*
MFG-E8 Mouse 31.25 - 2000 pg/mL DY2805 15 Plates
* DuoSet IC Kits are also available in 5 plate packs and 15 plate economy pack . Standard DuoSet Kits are also available in 45 plate Economy Pack .
OAS2ANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
OAS2 Human None HLS56/3 Mouse IgG2A IHC, IP MAB1925 100 µg
PARPANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PARPaa 71-329 (mouse)
Human/Mouse None Polyclonal Goat IgG IP, WB AF-600-NA 100 µg
PARPaa 71-329 (mouse)
Human/Mouse Biotin Polyclonal Goat IgG WB BAF600 50 µg
PARPaa 71-329 (mouse)
Mouse None 53015 Rat IgG2A WB MAB600 500 µg
PARP Multi-species None C2-10 Mouse IgG1 IHC, WB 4338-MC-50 50 µg
PBRANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PBR Human/Mouse/Rat None Polyclonal Rabbit IgG IHC, WB 6361-PC-100 100 µL
PBR Mouse/Rat None Polyclonal Rabbit IgG IHC, WB 6362-PC-100 100 µL
PoriminANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
Porimin Human None Polyclonal Total Goat IgG FC, WB AB3010 1 mg
Porimin Human None 297617 Mouse IgG2B FC, WB MAB3010 100 µg
R&D Systems Tools for Cell Biology Research™
All recombinant proteins lyophilized or in solution with a carrier protein are also available in a carrier-free form.To order the carrier-free form, add "/CF" to the end of the catalog number (e.g., 705-C8-010/CF).
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ss
PSRANTIBODIES
ANTIBODY SPECIES LABEL CLONE HOST/TYPE APPLICATIONS CATALOG # SIZE
PSR Mouse None Polyclonal Goat IgG WB AF1994 100 µg
PSR Mouse Biotin Polyclonal Goat IgG WB BAF1994 50 µg
Thymosin �1/PTMARECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Thymosin �1/PTMA Human Solution without a carrier protein None Synthetic 11976-1 500 µg
Transglutaminase 2RECOMBINANT & NATURAL PROTEINS
PROTEIN SPECIES FORM LABEL SOURCE CATALOG # SIZE
Transglutaminase 2 Human Solution without a carrier protein None Sf 21 4376-TG-050 50 µg
Apoptosis-related Molecule Families
For research use only. Not for use in diagnostic procedures. www.RnDSystems.com 117
For antibody application key, see page 120.
Other Apoptosis Molecules
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118 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373
4-Amino-1,8-Naphthalimide .................................. 14
6(5H)-Phenanthridinone .......................................... 14
6-Biotin-17-NAD.......................................................... 14
14-3-3 .............................................................................. 59
14-3-3b ........................................................................... 59
14-3-3e............................................................................ 59
14-3-3h ........................................................................... 59
14-3-3s ........................................................................... 60
14-3-3q ........................................................................... 60
14-3-3z ........................................................................... 60
4-1BB/TNFRSF9 ........................................................... 29
4-1BB Ligand/TNFSF9 ............................................... 29
4-Hydroxynonenal ..................................................... 75
53BP1 ............................................................................102
aA Crystallin/CRYAA .................................................. 80
AATK ..............................................................................115
aB Crystallin/CRYAB .................................................. 80
AIF .................................................................................... 71
Akt .................................................................................... 84
Akt1 ...........................................................................84-85
Akt2 ................................................................................. 85
Akt3 ................................................................................. 85
Annexin V ....................................................................115
Annexin V Assay .............................................................5
APAF-1 ............................................................................ 19
Apolipoprotein H/ApoH ........................................115
ApoStat Detection Kit ..................................................6
APRIL/TNFSF13 ............................................................ 30
ARC .................................................................................. 19
ASC .................................................................................. 19
ASK1 ................................................................................ 85
ATM ................................................................................102
Bad ................................................................................... 65
BAFF/TNFSF13B .......................................................... 30
BAFF R/TNFRSF13C .................................................... 31
Bag-1 ............................................................................... 65
BAK .................................................................................. 65
Bax .............................................................................65-66
Bcl-10 .............................................................................. 66
Bcl-2 ................................................................................. 66
Bcl-2 related protein A1 ........................................... 67
Bcl-w ................................................................................ 67
Bcl-x ................................................................................. 67
Bcl-xL ..........................................................................67-68
BCMA/TNFRSF17 ........................................................ 31
Benzamide .................................................................... 14
BID .................................................................................... 68
Bik-like Killer Protein ................................................. 68
BIM ................................................................................... 68
BNIP3............................................................................... 68
BNIP3L ............................................................................ 69
BOK .................................................................................. 69
C1D ................................................................................115
Calcein AM .......................................................................4
CaMKKa ......................................................................... 85
Carboxymethyl Lysine .............................................. 74
CARMA1/CARD11 ....................................................... 98
Caspase .......................................................................... 20
Caspase-1 ...................................................................... 20
Caspase-2 ...................................................................... 20
Caspase-3 ................................................................20-21
Caspase-4 ...................................................................... 21
Caspase-6 ...................................................................... 21
Caspase-7 ...................................................................... 21
Caspase-8 ...................................................................... 22
Caspase-9 ...................................................................... 22
Caspase-10 ..............................................................22-23
Caspase-12 .................................................................... 23
Caspase-13 .................................................................... 23
Catalase .......................................................................... 74
CBP ........................................................................102, 106
CD27/TNFRSF7 ............................................................ 32
CD27 Ligand/TNFSF7 ................................................ 32
CD2AP............................................................................. 60
CD30/TNFRSF8 ............................................................ 33
CD30 Ligand/TNFSF8 ..........................................33-34
CD40/TNFRSF5 ............................................................ 34
CD40 Ligand/TNFSF5 ................................................ 35
Cell Culture Control Slides ....................................... 14
cIAP .................................................................................. 25
cIAP-1/HIAP-2 .............................................................. 25
cIAP-2/HIAP-1 .............................................................. 25
Clusterin ......................................................................... 80
Cobalt Cation ............................................................... 14
CometAssays ...................................................................8
CometSlide ................................................................... 14
CRADD ............................................................................ 58
CREB ..............................................................................106
CRF21 ............................................................................115
Crk .................................................................................... 58
CRMB ............................................................................... 35
CTGF/CCN2 .................................................................115
Cyr61/CCN1 ................................................................115
Cytochrome c .........................................................71-72
Cytonin ........................................................................... 14
DAXX ............................................................................... 59
DcR3/TNFRSF6B .......................................................... 36
DcTRAIL R1/TNFRSF23 .............................................. 36
DcTRAIL R2/TNFRSF22 .............................................. 36
DePsipher Assay .............................................................6
DFF45/ICAD .................................................................. 60
DNA Laddering Detection Kits .................................9
DR3/TNFRSF25 ............................................................ 37
DR6/TNFRSF21 ............................................................ 37
EDA ............................................................................37-38
EDA-A2 ........................................................................... 38
EDAR ............................................................................... 38
eNOS .........................................................................75-76
ERK1 ................................................................................ 86
ERK1/ERK2 ...............................................................85-86
ERK2 ..........................................................................86-87
FADD ............................................................................... 59
Fas/TNFRSF6 ...........................................................38-39
Fas Ligand/TNFSF6..................................................... 40
FLIP ............................................................................23, 60
FosB/G0S3 ...................................................................106
GbL................................................................................... 87
GITR/TNFRSF18 ........................................................... 41
GITR Ligand/TNFSF18 .........................................41-42
Glutaredoxin 1 ............................................................. 74
Glutathione Peroxidase 1 ........................................ 74
Glutathione Peroxidase 3 ........................................ 74
Glyoxalase I ................................................................... 74
Granzyme A ................................................................113
Granzyme B ................................................................113
Granzyme D ................................................................113
Granzyme G ................................................................114
Granzyme H ................................................................114
GRP75/HSPA9B ............................................................ 80
GRP78/HSPA5............................................................... 80
GSK-3a ........................................................................... 87
GSK-3a/b ....................................................................... 87
GSK-3b ............................................................................ 87
HO-1/HMOX1/HSP32 ..........................................74, 80
HO-2/HMOX2 ............................................................... 74
Holocytochrome c...................................................... 72
Hrk.................................................................................... 69
HSP10/EPF..................................................................... 81
HSP20/HSPB6 ............................................................... 81
HSP27.............................................................................. 81
HSP40/DNAJB1 ............................................................ 81
HSP60.............................................................................. 81
HSP70.............................................................................. 82
HSP90.............................................................................. 82
Index
R&D Systems Tools for Cell Biology Research™
119
HSPA8/HSC71............................................................... 82
HSPH1 ............................................................................. 82
HTRA2/Omi .............................................................25, 72
HVEM/TNFRSF14 ......................................................... 42
IkB-a ................................................................................ 98
IkB-b ................................................................................ 98
IkB-e................................................................................. 98
IKKa ................................................................................. 98
IKKb.................................................................................. 98
IKKe .................................................................................. 99
IKKg .................................................................................. 99
iNOS................................................................................. 76
Jak1 .................................................................................. 88
Jak2 .................................................................................. 88
Jak3 .................................................................................. 88
JNK ................................................................................... 88
JNK1 ..........................................................................88-89
JNK1/JNK2 ..................................................................... 88
JNK2 ................................................................................ 89
JunB ...............................................................................106
LIGHT/TNFSF14 .....................................................42-43
Livin ...........................................................................25-26
LMTK2 ............................................................................. 89
Lymphotoxin ................................................................ 43
Lymphotoxin b/TNFSF3 ........................................... 43
Lymphotoxin b R/TNFRSF3 ..................................... 43
MAD1L1 .......................................................................106
Magnesium Cation ..................................................... 14
Manganese Cation ..................................................... 14
Max ................................................................................106
Mcl-1 ............................................................................... 69
MDM2 ...........................................................................102
MEK1 ............................................................................... 89
MEK1/MEK2 ............................................................89-90
MEK2 ............................................................................... 90
MFG-E8 .........................................................................116
MKK3 ............................................................................... 90
MKK3/MKK6 .................................................................. 90
MKK4 ............................................................................... 90
MKK6 ............................................................................... 90
MKK7 ............................................................................... 90
MLK3 ............................................................................... 91
MLK4a ............................................................................ 91
MSK1 ............................................................................... 91
MSK1/MSK2 .................................................................. 91
MSK2 ............................................................................... 91
MST1/STK4 .................................................................... 91
MST2/STK3 .................................................................... 91
MTT .....................................................................................3
c-Myc .............................................................................107
MyD88 ............................................................................ 61
Myeloperoxidase ........................................................ 75
NAIP ................................................................................. 26
NALP2 ............................................................................. 23
NeuroPore ..................................................................... 14
NFkB1 .................................................................... 99, 107
NFkB2 .................................................................... 99, 107
NGF R/TNFRSF16 ........................................................ 44
Nitric Oxide ................................................................... 75
Nitrotyrosine ................................................................ 75
nNOS ............................................................................... 76
OAS2..............................................................................116
Osteoprotegerin/TNFRSF11B ...........................44-45
OX40/TNFRSF4 ............................................................ 45
OX40 Ligand/TNFSF4 ..........................................45-46
p38 .............................................................................91-92
p38a ................................................................................ 92
p38b ................................................................................ 92
p38d ................................................................................ 92
p38g ................................................................................. 93
p53 ......................................................102-103, 107-108
p53R2 ............................................................................103
p70 S6 Kinase ............................................................... 93
p70 S6 Kinaseb ............................................................ 93
p300 ..............................................................................103
Park7/DJ-1 ..................................................................... 76
PARP ..............................................................................116
PARP Activated DNA .................................................. 14
PARP Activity Assay .......................................................7
PBR .................................................................................116
PDK-1 .............................................................................. 94
Peroxiredoxin 1 ........................................................... 76
Peroxiredoxin 2 ........................................................... 76
Peroxiredoxin 5 ........................................................... 76
Peroxiredoxin 6 ........................................................... 77
Peroxynitrite ................................................................. 14
PI 3-Kinase p85a ......................................................... 94
PI 3-Kinase p110.......................................................... 94
PI 3-Kinase p110b ....................................................... 94
PI 3-Kinase p110d ....................................................... 94
PI 3-Kinase p110g........................................................ 94
Pin1 ......................................................................... 99, 103
PKA C............................................................................... 94
PKA Ca ........................................................................... 94
PKA RIb ........................................................................... 94
Poly(ADP-ribose) Polymer/pADPr ........................ 14
PON2 ............................................................................... 77
PON3 ............................................................................... 77
Porimin .........................................................................116
Proteinase K .................................................................. 14
Proteome Profiler Antibody Arrays ................15-16
PSR .................................................................................117
RANK/TNFRSF11A ...................................................... 46
c-Rel ........................................................................ 98, 108
RelB ......................................................................... 99, 108
RELT/TNFRSF19L ......................................................... 46
Resazurin ..........................................................................4
Rictor ............................................................................... 95
RIP1 .................................................................................. 59
RSK ................................................................................... 95
RSK1 ................................................................................ 95
RSK1/RSK2 ..................................................................... 95
RSK2 ................................................................................ 95
RSK3 ................................................................................ 95
RSK4 ................................................................................ 96
RTVP-1 ..........................................................................103
SMAC/Diablo ..........................................................26, 72
SOD1/Cu-Zn SOD ....................................................... 77
SOD2/Mn-SOD ............................................................ 77
SOD3/EC-SOD .............................................................. 77
SODD/BAG4 .................................................................. 60
Src .................................................................................... 96
STAT1 .............................................................................108
STAT2 .............................................................................109
STAT3 .............................................................................109
STAT4 .............................................................................109
STAT5a ..........................................................................109
STAT5a/b .............................................................109-110
STAT5b ..........................................................................110
STAT6 .............................................................................110
Streptavidin-Fluorescein .......................................... 14
Streptavidin-HRP ........................................................ 14
SUMO1 ........................................................................... 99
SUMO2/3/4 .................................................................100
SUMO3 .........................................................................100
Superoxide Dismutase ............................................. 77
Survivin ....................................................................26-27
TACI/TNFRSF13B ......................................................... 47
TACS Blue Label Substrate ...................................... 14
TACS Red Label Substrate........................................ 14
TANK ................................................................................ 61
TdT Enzyme/Buffer .................................................... 14
TdT dNTP Mix ............................................................... 14
Thioredoxin-1 .............................................................. 77
Thioredoxin-2 .............................................................. 78
Thioredoxin-80 ............................................................ 78
Thioredoxin-like 5/TRP14 ........................................ 78
Thymosin a1/PTMA .................................................117
Tissue Control Slides ................................................. 14
TL1A/TNFSF15 ............................................................. 47
For research use only. Not for use in diagnostic procedures.
Index
120 U.S. & Canada Tel (800) 343-7475 | UK & Europe Tel +44 (0) 1235 529449 | China Tel (21) 52380373
R&D Systems Tools for Cell Biology Research™
TNF-a/TNFSF1A ....................................................48-50
TNF-b/TNFSF1B .....................................................50-51
TNF RI/TNFRSF1A .................................................51-52
TNF RII/TNFRSF1B ....................................................... 52
TOR .................................................................................. 96
TRADD ............................................................................ 59
TRAF-1 ............................................................................ 61
TRAF-2 ............................................................................ 61
TRAF-3 ............................................................................ 61
TRAF-4 ............................................................................ 62
TRAF-6 ............................................................................ 62
TRAIL/TNFSF10 ............................................................ 53
TRAIL R1/TNFRSF10A ................................................ 53
TRAIL R2/TNFRSF10B................................................. 54
TRAIL R3/TNFRSF10C ................................................ 54
TRAIL R4/TNFRSF10D ................................................ 55
TRAM/TICAM2 ............................................................. 62
TRANCE/RANK L/TNFSF11 .................................55-56
Transglutaminase 2 .................................................117
TROY/TNFRSF19 .......................................................... 56
TUNEL Labeling Kits ............................................10-13
TWEAK/TNFSF12 ...................................................56-57
TWEAK R/TNFRSF12 .................................................. 57
TXNDC5 .......................................................................... 78
Tyk2 ................................................................................. 96
Ubiquitin ......................................................................100
Ubiquitin+1 ................................................................100
UCP2............................................................................... 72
WISP-1/CCN4 ..............................................................104
XEDAR ............................................................................. 57
XIAP ................................................................................. 27
XTT ......................................................................................3
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Index continued
Antibody Application KeyAP ..........................Immunoaffinity Purification
B/N........................Blocking/Neutralizing
Adhesion Blockade
Blockade of Receptor-
Ligand Interaction
CD..........................Cell Depletion
Dot ........................Dot Blot
FA ...........................Functional Assay
ELISA Cap ............ ELISA Capture (Matched Pair)
ELISA Comp .......Competitive ELISA
ELISA Det ............ELISA Detection
(Matched Pair)
EMSA ....................Gel Supershift Assay
FC ..........................Flow Cytometry
Intracellular Staining by
Flow Cytometry
IF ............................Immunofluorescence
Immunocytochemistry
IHC ........................Immunohistochemistry
IP ...........................Immunoprecipitation
WB .........................Western Blot
R&D Systems Apoptosis Catalog
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