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“Zebra Corn (zb4)” By Andrew Greenwood, Julia J. Kunberger, & Martha Swenson Student Projects on Maize Mutants Middle School Gifted Science Class Fall 2004 Mentorship Program Maize Genetics with Biologist H. Bass School of Arts and Sciences (SAS) Charter School Leon County School System, Tallahassee, FL Credits Science Teacher: Joan Crow, SAS Science Teacher FSU Mentor: Hank W. Bass, Assistant Professor of Biological Science, FSU Assistants: Bobbye Hill Biological Research Scientist, Bass lab, FSU Debbie M. Figueroa, Graduate Student, Bass lab, FSU Brian C. Ring, Post-doctoral Research Associate, Bass lab, FSU Additional thanks to: Julia Winter, SAS Math teacher Amber N. Brown, Graduate Student, Bass lab, FSU Links The SAS school web page http://www.artsandsciences.leon.k12.fl.us/main/ The SAS Mentorship Fall ‘04 page http://www.cytomaize.org/outreach/sas/ The Maize-10-Maize project http://www.cytomaize.org/outreach/ Hank Bass laboratory home page http://bio.fsu.edu/bass/ email contact: [email protected]

“Zebra Corn (zb4)” · zb4_AG_JJK_MS, 1-13-05 Growth Chart The mode of inheritance for zb4 is recessive Date Height Width Mean H Color 9/10 30 mm 1 mm 48.7 mm Pale green. Very

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“Zebra Corn (zb4)”By Andrew Greenwood, Julia J.Kunberger, & Martha SwensonStudent Projects on Maize MutantsMiddle School Gifted Science ClassFall 2004 Mentorship ProgramMaize Genetics with Biologist H. BassSchool of Arts and Sciences (SAS) Charter SchoolLeon County School System, Tallahassee, FL

CreditsScience Teacher: Joan Crow, SAS Science TeacherFSU Mentor: Hank W. Bass, Assistant Professor of Biological Science, FSUAssistants: Bobbye Hill Biological Research Scientist, Bass lab, FSU

Debbie M. Figueroa, Graduate Student, Bass lab, FSUBrian C. Ring, Post-doctoral Research Associate, Bass lab, FSU

Additional thanks to: Julia Winter, SAS Math teacherAmber N. Brown, Graduate Student, Bass lab, FSU

LinksThe SAS school web page http://www.artsandsciences.leon.k12.fl.us/main/The SAS Mentorship Fall ‘04 page http://www.cytomaize.org/outreach/sas/The Maize-10-Maize project http://www.cytomaize.org/outreach/

Hank Bass laboratory home page http://bio.fsu.edu/bass/email contact: [email protected]

zb4_AG_JJK_MS, 1-13-05

Zebra CornBy Andrew Greenwood, Martha Swenson, and

Julia Kunberger

School of Arts & Sciences

zebra crossbands4 (zb4), AG, JJK, MS, 1-13-05

zb4_AG_JJK_MS, 1-13-05

About OurProject

Throughout the semester,we have been studyingmutant corn. We decided tostudy zebra Crossbands4(zb4), a mutant known for itsexotic stripes. This is ourproject.

Slender, so slender

Its stalk bends under dew—

Little yellow ear

-A Zen Poet

zb4_AG_JJK_MS, 1-13-05

What is Wrong?Our mutant is called “Zebra” because of it’s zebra-like stripes on the leaves.The mutation is called zebra crossbands4 (zb4).The mutation has a recessive mode of inheritance.The zb4 gene has not yet been cloned.

What causes these stripes?

zb4_AG_JJK_MS, 1-13-05

What causes these stripes?There are many different theories on what

causes the zebra-like stripes on the leaves.Scientists have not yet discovered the exactcause for the stripes. We have some simpletheories based on our knowledge.

zb4_AG_JJK_MS, 1-13-05* Alleles = different forms of the same gene

Genotype & Phenotype ChartGenotypeof individual

Phenotypeof individual

ZZHomozygousDominant2 Dominant alleles

Heterozygous1 Dominant allele1 recessive allele

Homozygousrecessive2 recessive alleles

Relationship of thepairs of alleles*

Normal leaves(wild-type)

Normal leaves(wild-type, carrier)

Striped leaves(mutant)

ZzzZzz

zb4_AG_JJK_MS, 1-13-05

DiagramYoung Leaf

Barely striped

Middle-Aged LeafLight stripes (developing)

Old LeafVibrant yellow & green stripes

zb4_AG_JJK_MS, 1-13-05

Growth Chart

The mode of inheritance for zb4 is recessive

Date Height Width Mean H Color9/10 30 mm 1 mm 48.7 mm Pale green. Very small and weak.

9/24 124 mm 15 mm 150 mm Red at base. Light green. NOT ZEBRA! Some leaves have dead patches.

10/11 198 mm 10 mm 190 mm Yellow, brown, and green. Stems: purple.Starting to shrivel turn brown.Starting to die!

Stopped growing and started dying.

zb4_AG_JJK_MS, 1-13-05

(1938) HK Hayes. Zebra Seedling, zb4, has beenlocated in chromosome 1.Maize Newsletter. Volume 12, page 1.

(1939) HK Hayes. Recent Linkage studies inmaize. III. Zebra Seedling-4.

Genetics. Volume 24, page 60-61.

(1932) HK Hayes. Zebra Crossbands.

Journal of Heredity. Volume 23, pages 415-419.

Discoverer and References

zb4_AG_JJK_MS, 1-13-05

The End