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Anxiety and the GLO1 gene
By Emma BaarUniversity of Wisconsin-
Madison
What is an anxiety disorder
anxietydisorderxblogspotcom
Current treatments TherapyMedication
wwwfithealthylivingcom
184aa
The GLO1 Gene
httpuploadwikimediaorgwikipediacommonsaaeGLO1_Homo_sapiens_small_fastgif
Human GLO1 Glyoxalase Domain
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG is a byproduct of glycolysis
Methyglyoxal(MG)
httpwww-rohansdsueducourseens304public_htmlsection1Glycolysishtm httpcommonswikimediaorg
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
What is an anxiety disorder
anxietydisorderxblogspotcom
Current treatments TherapyMedication
wwwfithealthylivingcom
184aa
The GLO1 Gene
httpuploadwikimediaorgwikipediacommonsaaeGLO1_Homo_sapiens_small_fastgif
Human GLO1 Glyoxalase Domain
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG is a byproduct of glycolysis
Methyglyoxal(MG)
httpwww-rohansdsueducourseens304public_htmlsection1Glycolysishtm httpcommonswikimediaorg
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Current treatments TherapyMedication
wwwfithealthylivingcom
184aa
The GLO1 Gene
httpuploadwikimediaorgwikipediacommonsaaeGLO1_Homo_sapiens_small_fastgif
Human GLO1 Glyoxalase Domain
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG is a byproduct of glycolysis
Methyglyoxal(MG)
httpwww-rohansdsueducourseens304public_htmlsection1Glycolysishtm httpcommonswikimediaorg
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
184aa
The GLO1 Gene
httpuploadwikimediaorgwikipediacommonsaaeGLO1_Homo_sapiens_small_fastgif
Human GLO1 Glyoxalase Domain
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG is a byproduct of glycolysis
Methyglyoxal(MG)
httpwww-rohansdsueducourseens304public_htmlsection1Glycolysishtm httpcommonswikimediaorg
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG is a byproduct of glycolysis
Methyglyoxal(MG)
httpwww-rohansdsueducourseens304public_htmlsection1Glycolysishtm httpcommonswikimediaorg
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
MG is a byproduct of glycolysis
Methyglyoxal(MG)
httpwww-rohansdsueducourseens304public_htmlsection1Glycolysishtm httpcommonswikimediaorg
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
What does GLO1 do
Methylglyoxal(MG)
D-lactate
GLO1
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
MG
MG
httpwwwpicstopincom300feelings-face-sethttp7C7Cstorestoysofthetradecom7Ccatalog7C1687jpg
GLO1 substrate works like anti-anxiety medication
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Proteins that regulate Dopamineinteract with GLO1
string-dborg
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
235aa
Glyoxalase domain
184aa
184aa
162aa
176aa
326aa
GLO1 is well conservedHuman GLO1
Mouse Glo1
Worm MCE-1
Fruit Fly CGI707
ArabidopsisATIG08110
Yeast GLO1
wwwncbinlmnihgov
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Mice exhibit anxious behavior when overexpressing GLO1 gene
psylabidvtw
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Elevated Plus Maze
cellularscaleblogspotcom berlinmouseclinicorg
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
MG is in manuka honey
Used as an antibacterial
httpbehealthy24comwwwbuyessentialoilsherecom
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Manuka honey may be a natural way to decrease anxiety
httpwwwfanpopcomhttpbehealthy24com
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Hypothesis 1
MG in the diet will reduce anxiety while GLO1 retains normal function
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Mice on diets with and without manukahoney
httpbehealthy24comwwwsimplyfortiescom
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
2D gel comparison and MALDI-TOF MS of GLO1 -Expected Results
wwwsimplyfortiescom
Honey diet
Control
wwwpiercenetcom
GLO1
GLO1
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Additional Points of Interest
wwwhoneyshopconz
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
HPLC of MG Mouse Behavior
Additional Points of Interest
ManukaHoney Diet
Control
berlinmouseclinicorg
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Further Studies ndash GLO1 in yeast
184aaHuman GLO1
326aaYeast GLO1
wwwtgacacuk
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Hypothesis 2
Slightly decreasing GLO1 can decrease anxiety without MG becoming toxic
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
GLO1 small molecule inhibitors
httpwwwmdpicom1420-3049171213740
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Microarray ndash GLO1 decreases with inhibitors
Drug 1
Drug 2
Drug 3
Control
Dose of Inhibitor
None Low Medium High
Decreasing GLO1
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Future Directions
bull Regulation of GLO1 expression
bull Phenotypes in other model organisms
bull MAOA and MAOB
wwwhoneyshopconz
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
Questions
httpwwwfanpopcom
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
ReferencesAl-Balas Q Hassan M Al-Oudat B Alzoubi H Mhaidat N Almaaytah A Generation of the First Structure-Based
Pharmacophore Model Containing a Selective ldquoZinc Binding Grouprdquo Feature to Identify Potential Glyoxalase-1 Inhibitors Molecules 2012 17(12)13740-13758
Anxiety Disorders National Institute of Mental Health Retrieved February 5 2013 from httpwwwnimhnihgovhealthtopicsanxiety-disordersindexshtml
Distler M G Plant L D Sokoloff G Hawk A J Aneas I Wuenschell G E et al (2012) Glyoxalase 1 increases anxiety by reducing GABAA receptor agonist methylglyoxal J Clin Invest 122 2306ndash2315 doi 101172JCI61319
Distler M G and A A Palmer Role of Glyoxalase 1 (Glo1) and Methylglyoxal (MG) in Behavior Recent Advances and Mechanistic Insights PubMed (2012) n pag NCBI Web 7 Feb 2013 doi 103389
Altschul SF Gish W Miller W Myers EW and Lipman DJ (1990)Basic local alignment search tool J Mol Biol 215 403-410
Sigrist CJA de Castro E Cerutti L Cuche BA Hulo N Bridge A Bougueleret L Xenarios I New and continuing developments at PROSITE Nucleic Acids Res 2012 doi 101093nargks1067PubMed 23161676 [Full text] [PDF version]
Glyoxalase System Clinical Sciences Research Institute Warwick Retrieved February 28 2013 from httpwww2warwickacukfacmedresearchcsriproteindamageresearchinterestglyoxalase_i_inhibitors
Percent Identity of Genomic DNA Sequences of Introns and Exons and of Amino Acid Sequences Np ndWeb 21 Feb 2013
STRING (Search Tool for the Retrieval of Interacting GenesProteins) Np nd Web 23 Apr 2013
